阅读说明：本技术 一种儿童配方羊奶粉及其制备方法 (Child formula goat milk powder and preparation method thereof ) 是由 刘海荣 刘园园 张旭普 付秀娟 贾晓江 于 2021-09-03 设计创作，主要内容包括：本发明公开了一种儿童配方羊奶粉及其制备方法,是取全脂羊乳粉、脱盐羊乳清粉、低聚果糖、低聚半乳糖、大豆油、异构化乳糖液等主料与复合维生素溶液、复合矿物质溶液、氢氧化钾溶液和维生素K-(2)溶液混合后,经均质、杀菌、浓缩、喷雾干燥,然后加入干混料,混匀制得。本发明通过添加合理配比的原料,在保证为儿童提供充足营养成分的基础上,还具备增进智力、提高免疫力、保护视力、促进骨骼发育、促进肠道健康的多重功效。本发明适用于制备儿童配方羊奶粉。(The invention discloses a formula goat milk powder for children and a preparation method thereof, wherein the formula goat milk powder is prepared by taking main materials such as full-cream goat milk powder, desalted goat whey powder, fructo-oligosaccharide, galacto-oligosaccharide, soybean oil, isomerized lactose liquid and the like, and a compound vitamin solution, a compound mineral solution, a potassium hydroxide solution and vitamin K 2 Mixing the solutions, homogenizing, sterilizing, concentrating, spray drying, adding dry mixture, and mixing. The invention adds the raw materials with reasonable proportion, and has the functions of improving intelligence, improving immunity, protecting vision and promoting bone development on the basis of ensuring that sufficient nutrient components are provided for childrenAnd multiple effects of promoting intestinal health. The invention is suitable for preparing the children formula goat milk powder.)

1. The children formula goat milk powder is characterized in that raw materials for preparing active ingredients of the children formula goat milk powder comprise the following components in parts by weight:

main materials: 400 parts of full-fat goat milk powder, 300 parts of desalted goat whey powder, 250 parts of defatted goat milk powder, 10-20 parts of fructo-oligosaccharide, 1-5 parts of N-acetylneuraminic acid, 0.1-1 part of yeast beta-glucan, 10-20 parts of crystalline fructose, 1-10 parts of phospholipid and 0.1-1 part of phosphatidylserine;

liquid material: 50-60 parts of galacto-oligosaccharide, 1-10 parts of isomerized lactose and 10-20 parts of soybean oil;

thirdly, dry mixing: 1-5 parts of docosahexaenoic acid, 0.1-1 part of arachidonic acid, 0.1-1 part of bifidobacterium Bb-12 bacterial powder and 0.1-1 part of bifidobacterium lactis HN019 bacterial powder;

fourthly, auxiliary material A: 1-5 parts of compound vitamin, 0.01-0.05 part of zeaxanthin and 0.5-1.5 parts of choline chloride;

auxiliary material B: 0.2-1.5 parts of composite mineral and 5-10 parts of calcium carbonate;

vitamin K₂ 0.1-1 part;

3-7 parts of potassium hydroxide.

2. The children's formula goat milk powder of claim 1, wherein the multivitamins comprise, in parts by weight: 2-2.5 parts of vitamin A, 0.03-0.04 part of vitamin D, 10-20 parts of vitamin E and 1-2 parts of vitamin B₁2-3 parts of vitamin B₆0.005-0.010 part of vitamin B₁₂250-350 parts of vitamin C, 10-20 parts of pantothenic acid, 0.02-0.03 part of biotin, 0.5-1 part of folic acid, 0.01-0.02 part of nicotinic acid, 0.15-0.20 part of taurine and 0.5-1 part of lutein.

3. The children's formula goat milk powder according to claim 1 or 2, wherein the minerals comprise in parts by weight: 3-5 parts of copper, 50-100 parts of iron, 50-100 parts of zinc and 0.05-0.15 part of selenium.

4. The children's formula goat milk powder according to claim 1 or 2, wherein the viable count of the bifidobacterium Bb-12 is 2-5 x 10¹⁰CFU/g, the viable count of the bifidobacterium lactis HN019 powder is 2-5 multiplied by 10¹⁰CFU/g。

5. A method for preparing a goat milk powder formulation for children according to any one of claims 1 to 4, comprising the following steps in sequence:

s1, completely dissolving the auxiliary material A in water to obtain a solution A';

completely dissolving the auxiliary material B in water to obtain a solution B';

collecting vitamin K₂Completely dissolving in water to obtain solution C';

completely dissolving potassium hydroxide in water to obtain a solution D';

mixing the main material, the liquid material, the solution A ', the solution B', the solution C 'and the solution D', homogenizing, sterilizing and concentrating to obtain concentrated milk;

and S2, after spray drying the concentrated milk, adding dry blend, and mixing uniformly to obtain the children formula goat milk powder.

6. The method for preparing children' S formula goat milk powder according to claim 5, wherein in step S1, the temperature of the mixed material is 48-58 ℃;

the homogenization is carried out, and the pressure is 150-220 bar;

the sterilization is to perform DSI sterilization for 5-24s at 85-95 ℃;

the density of the concentrated milk is 1045-³。

7. The method for preparing children' S formula goat milk powder according to claim 5 or 6, wherein the spray drying is performed by preheating the concentrated milk, filtering, pumping into a drying tower by a high pressure pump, and spray drying in step S2.

8. The method for preparing children's formula goat milk powder according to claim 7, wherein the preheating is to preheat the concentrated milk to 60-80 ℃;

the filtration adopts a duplex filter;

the pressure of the high-pressure pump is 170-290 bar;

the air inlet temperature of the drying tower is 160-210 ℃, and the air exhaust temperature is 60-110 ℃.

Technical Field

The invention belongs to the technical field of dairy product processing, and relates to a children formula milk powder, in particular to a children formula goat milk powder and a preparation method thereof.

Background

As is known, the dairy product is rich in most of nutrients required by human growth, can promote the growth and development of human brain, nerves, bones and immune system, can participate in various important physiological activities of human bodies, and has great benefits for the growth and development of infants and young children and maintaining the health of adults. At present, the production and consumption of dairy products all over the world are mainly cow milk, and account for about 95 percent of the total amount of dairy products. The protein structures of the goat milk and the cow milk are obviously different, the goat milk protein can form fine, soft and granular hydrogel similar to human milk protein under the action of gastric juice, the digestion and the absorption of a human body are facilitated, and the casein in the goat milk mainly comprises alpha-2S casein and beta-casein, and does not contain allergen alpha-1S casein in milk. Therefore, compared with cow milk, goat milk is more suitable for people with lactose intolerance to drink.

The childhood period is the most rapid period of physical development, and the period often needs higher nutrition level to maintain the requirement of physical development of children, and the growth of height and weight, the development of intelligence and eyesight, the improvement of digestive system and immune system all need the supply of high-quality nutrients.

Vitamin K₂Is a fat-soluble vitamin containing 2-methyl-1, 4-naphthoquinone ring and can remarkably promote Ca²⁺In the skeletonThe mineralization and the inhibition of the bone calcium loss comprehensively regulate and control the bone metabolic process. Vitamin K is a coenzyme for glutamate gamma-carboxylase, carboxylating glutamate residues to gamma-carboxyglutamic acid (osteocalcin), the level of which directly determines the concentration of carboxylated osteocalcin. Osteocalcin is a key factor of bone calcification, has special affinity with hydroxyapatite, and has binding firmness influenced by carboxylation degree, and only carboxylated osteocalcin can react with Ca²⁺In combination with achieving bone mineralization, without vitamin K, calcium regulation will be interrupted;

phosphoesterylserine can be synthesized by human body by using serine, which can affect the transmission of chemical information in brain, and help brain cells to store and read data, and is an important nutrient element for maintaining normal memory, response and healthy mood of brain. When the content of acetylcholine in brain is increased, the information transmission speed among brain nerve cells is increased, and the physiological functions of the brain nerve cells include improving the memory of brain, enhancing intelligence and promoting brain development;

the yeast beta-glucan has an immunoregulation effect, and can be combined with macrophages in a human body to activate the macrophages; can activate lymphocytes to play the role of immunopotentiator; can secrete some immune factors for activating immune cells so as to enhance the function of an immune system, and therefore can be used as an immunostimulant;

zeaxanthin and lutein are important carotenoid components that are specifically highly concentrated in specific ocular tissues, can absorb short wavelength visible light entering the eye, and protect the visual receptors from damaging blue light;

n-acetylneuraminic acid, also called sialic acid, is present in high amounts in the central nervous system of mammals and can cause long-term irreversible effects on the brain during critical phases of brain development. Sialic acid is mainly present in gangliosides, which are involved in neuronal growth, synaptic plasticity and regulation of neurotransmitter transmission, and thus plays a crucial role in brain development;

the galacto-oligosaccharide and fructo-oligosaccharide are functional oligosaccharides which are not absorbed by digestive enzymes in human body, and can selectively promote the proliferation of probiotics in intestinal tract, inhibit the proliferation of pathogenic bacteria and maintain the balance of intestinal flora.

Disclosure of Invention

The invention aims to provide a formula goat milk powder for children, which has the purposes of promoting intestinal health, improving immunity, protecting eyesight, promoting bone growth and promoting brain development;

the invention also aims to provide a preparation method of the children formula goat milk powder.

In order to achieve the purpose, the invention adopts the technical scheme that:

the child formula goat milk powder comprises the following raw materials of active ingredients in parts by weight:

main materials: 400 parts of full-fat goat milk powder, 300 parts of desalted goat whey powder, 250 parts of defatted goat milk powder, 10-20 parts of fructo-oligosaccharide, 1-5 parts of N-acetylneuraminic acid, 0.1-1 part of yeast beta-glucan, 10-20 parts of crystalline fructose, 1-10 parts of phospholipid and 0.1-1 part of phosphatidylserine;

liquid material: 50-60 parts of galacto-oligosaccharide, 1-10 parts of isomerized lactose and 10-20 parts of soybean oil;

thirdly, dry mixing: 1-5 parts of docosahexaenoic acid, 0.1-1 part of arachidonic acid, 0.1-1 part of bifidobacterium Bb-12 bacterial powder and 0.1-1 part of bifidobacterium lactis HN019 bacterial powder;

fourthly, auxiliary material A: 1-5 parts of compound vitamin, 0.01-0.05 part of zeaxanthin and 0.5-1.5 parts of choline chloride;

auxiliary material B: 0.2-1.5 parts of composite mineral and 5-10 parts of calcium carbonate;

vitamin K₂0.1-1 part;

3-7 parts of potassium hydroxide.

As a limitation, the vitamin complex comprises the following components in parts by weight: 2-2.5 parts of vitamin A, 0.03-0.04 part of vitamin D, 10-20 parts of vitamin E and 1-2 parts of vitamin B₁2-3 parts of vitamin B₆0.005-0.010 part of vitamin B₁₂250-350 parts of vitamin C, 10-20 parts of pantothenic acid, 0.02-0.03 part of biotin, 0.5-1 part of folic acid and 0.01-0.02 part of pantothenic acidNicotinic acid, 0.15-0.20 part of taurine and 0.5-1 part of lutein.

As another limitation, the minerals comprise, in parts by weight: 3-5 parts of copper, 50-100 parts of iron, 50-100 parts of zinc and 0.05-0.15 part of selenium.

As a third limitation, the number of viable bacteria in the powder of the bifidobacterium Bb-12 is 2-5 multiplied by 10¹⁰CFU/g, the viable count of the bifidobacterium lactis HN019 powder is 2-5 multiplied by 10¹⁰CFU/g。

The invention also provides a preparation method of the children formula goat milk powder, which comprises the following steps in sequence:

s1, completely dissolving the auxiliary material A in water to obtain a solution A';

completely dissolving the auxiliary material B in water to obtain a solution B';

collecting vitamin K₂Completely dissolving in water to obtain solution C';

completely dissolving potassium hydroxide in water to obtain a solution D';

mixing the main material, the liquid material, the solution A ', the solution B', the solution C 'and the solution D', sterilizing and concentrating to obtain concentrated milk;

and S2, after spray drying the concentrated milk, adding dry blend, and mixing uniformly to obtain the children formula goat milk powder.

As one limitation, in step S1, the temperature of the mixed material is 48-58 ℃;

the homogenizing pressure is 150-220 bar;

the sterilization is that DSI sterilization is carried out for 5-24s at the temperature of 85-95 ℃;

the density of the concentrated milk is 1045-³。

As another limitation, in step S2, the spray drying is performed by preheating the concentrated milk, filtering, and pumping into a drying tower by a high pressure pump;

the dry blending comprises premixing and large dry blending;

the premixing time is 120 s;

the large dry mixing time was 120 s.

As a further limitation, the preheating is to preheat the concentrated milk to 60-80 ℃;

the filtration adopts a duplex filter;

the pressure of the high-pressure pump is 170-290 bar;

the air inlet temperature of the drying tower is 160-210 ℃, and the air exhaust temperature is 60-110 ℃.

Due to the adoption of the technical scheme, compared with the prior art, the invention has the technical progress that:

firstly, the goat milk in the formula for children provided by the invention replaces cow milk in the traditional formula with goat milk, so that the problem that children with lactose intolerance cannot normally take dairy products is solved;

secondly, the reasonable proportion of the full-fat goat milk powder, the desalted goat whey powder and the degreased goat milk powder is added into the goat milk powder for children, so that reasonable proportion of protein and fat can be added into the goat milk powder for children, and the energy and the material of the goat milk powder for children are sufficient;

thirdly, four prebiotics (galacto-oligosaccharide, fructo-oligosaccharide, polyfructose and isomerized lactose liquid) and two probiotics (bifidobacterium Bb-12 and bifidobacterium lactis HN019) are added into the children formula goat milk powder, and the prebiotics can promote the good growth of the probiotics; the growth promoting capability of the probiotics by using multiple prebiotics together is better than that of a single prebiotic, and the prebiotics have synergistic beneficial effects and are better than any effect when used alone in the process of regulating the intestinal health;

the reasonable proportion of the zeaxanthin and the lutein is added into the formula goat milk powder for children, the lutein and the zeaxanthin 2 carotenoid exist in the retina and the crystalline lens, the two are the most effective antioxidants for preventing lipid peroxidation and oxidation in eyes, the beneficial effects of synergy are also realized between the two, the oxygen can return to the ground state from a singlet state by using the formula goat milk powder for children together, so that the retina is protected, the process can be repeated, and the effect of the formula goat milk powder for children when the two are used together in the process of protecting the eyesight is better than that when the two are used independently;

the goat milk powder prepared according to the formula for children provided by the invention can promote intestinal health, and also can help children to promote intelligence, improve immunity, protect eyesight and promote bone development;

sixthly, according to the preparation method of the children formula goat milk powder, provided by the invention, docosahexaenoic acid, arachidonic acid, bifidobacterium Bb-12 and bifidobacterium lactis HN019 are added in a dry mixing stage, so that the problems of denaturation and inactivation of the bifidobacterium Bb-12 and the bifidobacterium lactis HN019 and reduction of oxidation of the docosahexaenoic acid and the arachidonic acid can be avoided.

DHA (docosahexaenoic acid) and ARA (arachidonic acid) are greatly influenced by other strengthening substances such as oxygen, iron ions, zinc ions and the like. The dry addition of ARA and DHA can avoid the loss condition caused in the wet processing technology.

The invention is suitable for preparing the children formula goat milk powder.

Detailed Description

The present invention will be described in further detail with reference to specific examples. It should be understood that the described embodiments are only for illustrating the invention and do not limit the invention.

Example 1 preparation method of children formula goat milk powder

The embodiment includes steps S1 and S2 performed in sequence, where the specific contents of S1 and S2 are as follows:

s1, weighing 3kg of compound vitamin (comprising 17.85g of vitamin A, 0.32g of vitamin D, 162.28g of vitamin E and 11.36g of vitamin B)₁16.23g vitamin B₆0.05g of vitamin B₁₂2677.59g vitamin C, 97.39g pantothenic acid, 0.16g biotin, 7.30g folic acid, 0.08g nicotinic acid, 1.30g taurine and 8.11g lutein), 50g zeaxanthin and 0.7kg choline chloride as auxiliary material A₁Dissolving it in 250L water to obtain solution A₁'; in addition, the first and second substrates are,

weighing 0.5kg of composite mineral (composed of 8.67g of copper, 202.2g of iron, 288.85g of zinc and 0.29g of selenium) and 8kg of calcium carbonate as auxiliary materials B₁Dissolving it in 400L water to obtain solution B₁'; in addition, the first and second substrates are,

weighing vitamin K₂0.5kg of water, completely dissolved in 250L of water to obtain solution C₁'; in addition, the first and second substrates are,

weighing 3kg of potassium hydroxide, and completely dissolving in 250L of water to obtain solution D₁'; in addition, the first and second substrates are,

weighing main materials: 380kg of full-fat goat milk powder, 200kg of desalted goat whey powder, 250kg of defatted goat milk powder, 18kg of fructo-oligosaccharide, 1kg of N-acetylneuraminic acid, 1kg of yeast beta-glucan, 16kg of crystalline fructose, 5kg of phospholipid and 1kg of phosphatidylserine; in addition, the first and second substrates are,

weighing liquid materials: 50kg of galacto-oligosaccharide, 1kg of isomerized lactose and 16kg of soybean oil;

then, the main material, the liquid material and the solution A are mixed₁', solution B₁', solution C₁' and solution D₁Mixing materials at 55 deg.C, homogenizing at 151bar, sterilizing at 90 deg.C in DSI sterilizer for 15s, introducing into flash evaporator, introducing into falling film evaporator via duplex filter, and concentrating to obtain concentrated milk X₁Concentrated milk X₁Has a density of 1143kg/m³；

S2, concentrated milk X₁Preheating to 76 deg.C, filtering with duplex filter, pumping into drying tower with inlet air temperature of 198 deg.C and exhaust air temperature of 97 deg.C under 225bar by high pressure pump, and spray drying to obtain semi-finished powder X₂Adding 2kg docosahexaenoic acid, 1kg arachidonic acid, 0.1kg Bacillus bifidus Bb-12 powder, 0.5kg Bifidobacterium lactis HN019 powder and 15kg semi-finished product powder X₂Dry mixing for 120s to obtain semi-finished powder X₃Then, X is added₃With the remainder of X₂Dry mixing for 120s to obtain the goat milk powder Y for children₁；

Wherein the compound vitamin comprises vitamin A, vitamin D, vitamin E, and vitamin B₁Vitamin B₆Vitamin B₁₂Vitamin C, pantothenic acid, biotin, folic acid, nicotinic acid, taurine and lutein in a weight ratio of 2.2:0.04:20:1.4:2:0.006:330:12:0.02:0.9:0.01: 0.16: 1;

wherein, the weight ratio of copper, iron, zinc and selenium in the composite mineral is 3:70:100: 0.1.

Examples 2-6 preparation of goat milk powder for Children formula

Examples 2 to 6 are methods for preparing a goat milk powder for children respectively, and the steps are substantially the same as those of example 1, except for the differences in the amounts of raw materials and process parameters, which are detailed in table 1:

TABLE 1 summary of the process parameters of examples 2-6

Wherein, the dosage of each component in the compound vitamin in the examples 2-6 is shown in the table 2:

TABLE 2 amounts of the components of the multivitamins of examples 2-6

Wherein, the amounts of the components in the composite minerals of examples 2-6 are shown in Table 3:

TABLE 3 amounts of the components of the composite minerals of examples 2-6

The contents of other portions of examples 2 to 6 are the same as those of example 1.

Example 7 measurement results of body growth promoting effects of child formula goat milk powder

For the experiment for measuring the body growth promoting effect of the children formula goat milk powder prepared in example 1, the specific detection method is as follows:

experimental animals: selectingTaking 4-week-old male KM mice at the stage of establishing intestinal flora, randomly dividing the mice into a whole goat milk powder group (group A), a child formula cow milk powder group (group B) and a child formula goat milk powder group (group C)₁Groups), 10 per group;

the experimental method comprises the following steps: pair A, B, C₁Groups were treated with the following tests, respectively:

group A: the common feed is freely eaten, drunk and mixed with the whole sheep milk powder to be infused into the stomach;

group B: the common feed is freely eaten and drunk, and the children formula milk powder is mixed with the common feed and then is infused into the stomach;

C₁group (2): the common feed is freely eaten and drunk, and the child formula goat milk powder prepared in the embodiment 1 is mixed with water and then poured into the stomach;

intragastric administration dosage: weighing 0.2g of milk powder each time, dissolving in 0.5mL of distilled water, and performing intragastric administration for 1 time every day;

the average body weight and body length of each group of mice after 0, 7, 14, 21 and 28d of the initial experiment are recorded, and the specific data are shown in table 4:

TABLE 4 measurement results and analysis of body weight and body length of each group of mice

Note: different letters in the same column indicate significant difference (P < 0.05); same letter in same column indicates no significant difference (P >0.05)

As can be seen from Table 4: in experiment 0d, three indexes, group A, group B and group C₁There was no significant difference in the groups (P < 0.05). In experiment 28d, three groups of indexes, group A and group B, are compared with group C₁There was a significant difference between groups (P <0.05) and no significant difference between groups A and B. The children formula goat milk powder and the children formula cow milk powder have better promotion effect on body growth than the pure whole goat milk powder.

Example 8 assay results for Constipation relief in Children's formula goat milk powder

In the test for testing the constipation relieving effect of the goat milk powder for children prepared in example 2, the specific test method is as follows:

experimental animals: selectingTaking 4-week-old male KM mice at the stage of establishing intestinal flora, randomly dividing the mice into a whole goat milk powder group (group A), a child formula cow milk powder group (group B) and a child formula goat milk powder group (group C)₂Groups), 10 per group;

the experimental method comprises the following steps: pair A, B, C₂Groups were treated with the following tests, respectively:

group A: the common feed is freely eaten, drunk and mixed with the whole sheep milk powder to be infused into the stomach;

group B: the common feed is freely eaten and drunk, and the children formula milk powder is mixed with the common feed and then is infused into the stomach;

C₂group (2): the common feed is freely eaten and drunk, and the child formula goat milk powder prepared in the embodiment 2 is mixed with water and then poured into the stomach;

intragastric administration dosage: weighing 0.2g of milk powder each time, dissolving in 0.5mL of distilled water, and performing intragastric administration for 1 time every day;

in the 28 th experiment, after the gavage is carried out for 1h, 10 spherical color particles made of PP (polypropylene) with the thickness of 0.8mm are gavage for each mouse by using a flexible gavage needle, and the time for seeing the color particles in defecation for the first time is recorded; feces are collected 6h after the gavage, the water content of the feces is measured, and the experimental result is shown in table 5:

the water content (E) of the excrement is calculated according to the formula: e ═ M₁-M₂)/M₁×100％

In the formula: m₁The weight of wet feces; m₂Is the weight of dry excrement

TABLE 5 stool assay results and analysis

Group of	First defecation time (g)	Moisture content of feces (%)	Number of plastic particles discharged
				Group A	234±32.41c	27.88±2.55a	2.55±1.37a
Group B	220±30.23b	30.34±3.29b	3.78±1.22b
				C2Group of	200±26.15a	35.76±3.02c	5.90±0.52c

Note: different letters in the same column indicate significant difference (P < 0.05); same letter in same column indicates no significant difference (P >0.05)

As can be seen from Table 5: group A, group B and group C₂Groups were all significantly different. C₂Compared with the group A and the group B, the group A has short defecation time, high water content and large discharge quantity of plastic particles; the goat milk powder formulated by children has a promoting effect on gastrointestinal peristalsis of mice; and the group B is commercial formula milk powder for children, does not contain probiotics and various prebiotics, is not beneficial to defecation and is easy to cause constipation.

Example 9 test results of improving immunity of children's formula goat milk powder

In the experiment for measuring the immunity improvement of the children formula goat milk powder prepared in example 3, the specific detection method is as follows:

experimental animals: selecting 4-week-old male KM mice at the stage of establishing intestinal flora, and randomly dividing the mice into a sheep whole milk powder group (group A), a children formula milk powder group (group B) and a mother groupExperimental Children formula goat milk powder group (C)₃Groups), 10 per group;

the experimental method comprises the following steps: pair A, B, C₃Groups were treated with the following tests, respectively:

group A: the common feed is freely eaten, drunk and mixed with the whole sheep milk powder to be infused into the stomach;

group B: the common feed is freely eaten and drunk, and the children formula milk powder is mixed with the common feed and then is infused into the stomach;

C₃group (2): the common feed is freely eaten and drunk, and the child formula goat milk powder prepared in the embodiment 3 is mixed with water and then poured into the stomach;

intragastric administration dosage: 0.2g of milk powder is weighed each time, dissolved in 0.5mL of distilled water and then is perfused into the stomach, and the stomach is perfused for 1 time every day.

At 28d, after fasting for 12h, 20 μ L of blood was taken from the left-eye venous plexus and IgA, IgM, IgG were measured by a fully automatic biochemical analyzer, with the results shown in table 6:

TABLE 6 Thymus, spleen, immunoglobulin assay results and analysis

Group of	Spleen index	Index of thymus	IgA(g/L)	IgM(g/L)	IgG(g/L)
						Group A	0.020±0.005a	0.026±0.003a	0.025±0.01a	0.251±0.03a	0.088±0.03a
Group B	0.026±0.002b	0.030±0.002b	0.033±0.00b	0.329±0.07b	0.096±0.03ab
						C3Group of	0.028±0.003b	0.032±0.004b	0.036±0.01b	0.346±0.08b	0.107±0.02b

Note: different letters in the same column indicate significant difference (P < 0.05); the same letters in the same column indicate no significant difference (P > 0.05).

As can be seen from Table 6: spleen and thymus are the main immune organs, immunoglobulin exists in plasma and is a type of globulin with antibody activity or chemical structure similar to that of antibody, the immunoglobulin is divided into five types of IgG, IgA, IgM, IgD and gE, and the immunoglobulin is mainly IgG, IgA and IgM, and the spleen index, thymus index and immunoglobulin content of the mouse are analyzed: c₃The indexes of the group are higher than those of the group B and the group A, the group A and the group B have no significant difference, but the indexes are different from those of the group C₃The obvious difference of the groups shows that both the children formula goat milk powder and the children formula milk powder are beneficial to improving immunity, and the children formula goat milk powder is superior to the children formula milk powder.

Example 10 results of a determination that a child's formula goat milk powder promotes bone development

For the test for promoting bone development of the children formula goat milk powder prepared in example 4, the specific detection method is as follows:

experimental animals: selecting 4-week-old male KM mice at the stage of establishing intestinal flora, and randomly dividing the mice into a whole goat milk powder group (group A), a child formula cow milk powder group (group B) and a child formula goat milk powder group (group C)₄Groups), 10 per group;

the experimental method comprises the following steps: pair A, B, C₄Groups were treated with the following tests, respectively:

group A: the common feed is freely eaten, drunk and mixed with the whole sheep milk powder to be infused into the stomach;

group B: the common feed is freely eaten and drunk, and the children formula milk powder is mixed with the common feed and then is infused into the stomach;

C₄group (2): the common feed is freely eaten and drunk, and the child formula goat milk powder prepared in the embodiment 4 is mixed with water and then poured into the stomach;

intragastric administration dosage: weighing 0.2g of milk powder each time, dissolving in 0.5mL of distilled water, and performing intragastric administration for 1 time every day;

at experiment 28d, the cervical vertebrae were dislocated and sacrificed, the mice were dissected, the left femur was taken, the weight and length were measured with a vernier caliper, the thymus and spleen were taken to measure the wet weight, and further the spleen index and thymus index were measured:

spleen index ═ (spleen weight/mouse body weight) × 10

Thymus index (thymus weight/mouse weight) × 10

Determination of bone index: taking a bone sample, removing attached muscle tissues and the like, soaking in anhydrous ether for 12h for degreasing, placing in a drying box at 105 ℃ for 72h, balancing at room temperature for 24h, then weighing the bone by using a balance, measuring the length of the femur by using a vernier caliper, placing the femur in an oven for drying until the constant weight is achieved, and measuring the dry weight of the bone; then, the femur is put into a crucible, carbonized to be smokeless, put into a muffle furnace at 550 ℃ for ashing for 12h, the weight of the ash is constant, the ash weight is measured, statistical treatment is carried out by applying SPSS 13.0 software, and the experimental results are shown in Table 7:

table 7 left femur measurements and analysis

Group of	Femur weight (g)	Femur length (g)	Dry weight of femur (g)	Ash weight (g)
					Group A	85.13±2.50a	16.63±0.11a	38.79±0.97a	23.44±0.34a
Group B	87.63±3.01b	17.93±0.16b	41.64±1.35b	26.99±0.38b
					C4Group of	88.70±2.62c	18.52±0.13c	43.33±1.70c	28.06±0.27c

Note: different letters in the same column indicate significant difference (P < 0.05); the same letters in the same column indicate no significant difference (P > 0.05).

As can be seen from Table 7: group A, group B and group C₄Groups differed significantly. C₄The indexes of femoral weight, femoral length, femoral dry weight and ash content of the group A are higher than those of the group B and the group B, and the index of the group A is lower than that of the group B and the index of the group C₄And the formula shows that the children formula goat milk powder is superior to the commercially available children formula cow milk powder and pure goat whole milk powder in the aspect of promoting bone development.

Example 11 results of determining the brain development of the child formula goat milk powder

For the experiment for determining the brain development promotion of the children formula goat milk powder prepared in example 5, the specific detection method is as follows:

experimental animals: selecting 4-week-old male KM mice at the stage of establishing intestinal flora, and randomly dividing the mice into a whole goat milk powder group (group A), a child formula cow milk powder group (group B) and a child formula goat milk powder group (group C)₅Groups), 10 per group;

the experimental method comprises the following steps: pair A, B, C₅Groups were treated with the following tests, respectively:

group A: the common feed is freely eaten, drunk and mixed with the whole sheep milk powder to be infused into the stomach;

group B: the common feed is freely eaten and drunk, and the children formula milk powder is mixed with the common feed and then is infused into the stomach;

C₅group (2): the common feed is freely eaten and drunk, and the child formula goat milk powder prepared in the example 5 is mixed with water and then poured into the stomach;

intragastric administration dosage: 0.2g of milk powder is weighed each time, dissolved in 0.5mL of distilled water and then is perfused into the stomach, and the stomach is perfused for 1 time every day.

After 28d, performing Morris water maze experiments on each group of mice, wherein the determination contents comprise a positioning navigation experiment and a space exploration experiment;

the positioning navigation experiment is carried out for 5 days, the mice are trained for 4 times every day, the mice are respectively put into water from 4 quadrant fixing positions, and after all the mice finish one training, the next training is carried out for more than 20 min. Recording the time (namely the escape latency) required by finding the platform in 60s of the mouse, and if the mouse does not find the platform in 60s, guiding the platform to the platform and placing for 5s, wherein the latency is recorded as 60 s; the space exploration experiment is carried out on the 6 th day, the underwater platform is removed, the opposite side quadrant of the platform in the quadrant is taken as a water inlet point, the swimming speed of the mouse in 60s, the frequency of passing through the platform, the activity time of the platform in the quadrant and the time of passing through the platform for the first time are recorded, and the experiment results are shown in tables 8 and 9:

TABLE 8 comparison of incubation periods for localization voyage experiments

As can be seen from Table 8: with the increase of training days, the escape latency in the water maze experiment of each group of mice is shortened, which indicates that the training is effective, however, the daily data shows that the escape latency among the mice of each group is compared, and the difference is not significant (P)>0.05) but with three days of training, the latency decreased rapidly from 30s or more to 23s or less, and C₅Group < B < A;

TABLE 9 spatial memory comparison

As can be seen from Table 9: the swimming speed, the times of passing through the platform, the moving time in the quadrant of the platform and the time of passing through the platform for the first time of the mouse within 60s reflect the space memory capacity of the mouse, the swimming speed, the times of passing through the platform, the percentage of the third quadrant time, C₅Group B and group A have significant difference (P)>0.05), first pass plateau time, C₅The group has significant difference (P) compared with the group B and the group A of the control group>0.05)，C₅The four indexes of the group are all ahead of the group B and the group A;

the principle of the Morris water maze experiment is that the mouse aversion to stay in water and the instinctive reaction that the physical strength is not allowed to be in water for a long time are used as stimulation, so that the mouse needs to find a platform in time to stay, the learning memory capacity and the space position cognitive capacity of the mouse can be evaluated through data after training, and the formula goat milk powder group for children is superior to a common commercially available cow milk powder group and a pure goat whole milk powder control group through comparison of latency and space memory.