阅读说明：本技术 包含有机酸钙和氨基酸的饲料用添加剂组合物及其制备方法 (Feed additive composition containing organic acid calcium and amino acid and preparation method thereof ) 是由 季绍东 万毅 史自强 高伟 宋伟光 于 2021-03-17 设计创作，主要内容包括：本发明涉及一种由万寿菊发酵液制备得到的饲料添加剂,所述饲料添加剂包含有机酸钙和氨基酸。优选的,所述由万寿菊发酵液制备得到的饲料添加剂中,乳酸钙和乙酸钙的重量比为3:1-1:3,乳酸钙与缬氨酸的重量比为200:1-30:1。本发明有效地实现了对万寿菊发酵水综合利用,解决了现有技术中对于处理万寿菊发酵水难度大,成本高的问题。由万寿菊发酵水制备得到的饲料添加剂可用于饲料中。通过调整所述饲料添加剂中,乳酸钙和乙酸钙的重量比为3:1-1:3,乳酸钙与缬氨酸的比例为200:1-30:1时,能够获得较好的促进动物生长的技术效果。在同等添加剂量下,比三种成分单独使用时有更好的促生长作用。(The invention relates to a feed additive prepared from marigold fermentation liquor, which comprises organic acid calcium and amino acid. Preferably, in the feed additive prepared from the marigold fermentation liquor, the weight ratio of calcium lactate to calcium acetate is 3:1-1:3, and the weight ratio of calcium lactate to valine is 200:1-30: 1. The method effectively realizes the comprehensive utilization of the marigold fermentation water and solves the problems of high difficulty and high cost in the treatment of the marigold fermentation water in the prior art. The feed additive prepared from marigold fermented water can be used in feed. By adjusting the weight ratio of calcium lactate to calcium acetate in the feed additive to 3:1-1:3 and the weight ratio of calcium lactate to valine to 200:1-30:1, a good technical effect of promoting animal growth can be obtained. Under the condition of the same additive amount, the growth promoting effect is better than that of the three components which are used independently.)

1. A feed additive prepared from marigold fermentation liquor comprises organic acid calcium and amino acid.

2. The feed additive prepared from marigold fermentation broth according to claim 1, wherein the calcium organic acid is calcium lactate and/or calcium acetate.

3. A feed additive prepared from a marigold fermentation broth according to claim 1 or 2, wherein the amino acid is valine.

4. A feed additive prepared from a marigold fermentation broth according to claim 1 or 2, wherein the weight ratio of calcium lactate to calcium acetate is 3:1-1:3, and the ratio of calcium lactate to valine is 200:1-30: 1.

5. The feed additive prepared from marigold fermentation broth according to claim 1 or 2, wherein the feed additive further comprises one or more ingredients selected from a carrier and an emulsifier.

6. A method for preparing feed additive from marigold fermentation liquor comprises adding calcium hydroxide or calcium oxide into marigold fermentation liquor as raw material, adjusting pH to 7-9, concentrating, and dehydrating.

7. A method of producing a feed supplement from marigold fermentation broth according to claim 6, further comprising adding exogenous lactic acid, acetic acid, valine to the feed supplement produced from marigold fermentation broth to adjust the weight ratio of calcium lactate, calcium acetate, and valine in the feed supplement.

8. The method for preparing feed additive from marigold fermentation broth according to claim 6 or 7, wherein the weight ratio of calcium lactate, calcium acetate and valine in the feed additive is adjusted to 3:1-1:3 and 200:1-30: 1.

9. A method for treating marigold fermentation liquor comprises the following steps:

step (1), adding calcium hydroxide or calcium oxide into marigold fermentation liquor, adjusting the pH value to 7-9, concentrating and dehydrating;

optionally, step (2), adding feed additives into the concentrated and dehydrated composition.

10. The method for treating marigold fermentation broth according to claim 9, wherein the feed additive is selected from one or a mixture of two or more of lactic acid, acetic acid and valine.

Technical Field

The invention belongs to the field of feed additives, and particularly relates to a feed additive composition containing organic acid calcium and valine and a preparation method thereof.

Background

The feed additive is a small amount of nutritive or non-nutritive substance added into the feed for improving the utilization rate of the feed, ensuring or improving the quality of the feed, promoting the production of the raised animals and ensuring the health of the raised animals. The nutritional additives of the feed usually comprise amino acid additives, and the effects of the nutritional additives are mainly to improve the amino acid balance of the feed, promote the growth of animals and improve the utilization rate of the feed; protein resources are saved; improving meat quality and promoting trace element absorption; relieving stress reaction and improving disease resistance. Valine, also known as aminoisovaleric acid, is used as a nutritive substance in the food and feed industries. Organic acid salts are also common additives in feed, which are capable of providing high quality minerals to the feed.

Marigold flowers are used as ideal raw materials for industrially extracting lutein, in the production and processing processes, firstly, lactic acid bacteria are added into fresh marigold flowers for fermentation, and marigold fermentation water generated by fermentation is high-concentration organic wastewater which is brownish yellow, strong in acidity and smelly, has CODcr up to 50000-. The marigold fermentation liquor has complex components and great processing difficulty, and the problem of how to process the marigold fermentation water and reuse the water with value is one of the problems in the lutein extraction industry at present.

The inventor of the application finds that the marigold fermentation liquor contains abundant organic acids such as lactic acid and acetic acid, volatile organic acids such as acetic acid cannot be concentrated in an evaporation mode, are not easy to volatilize after being neutralized by acid and alkali to become organic acid salts, and are convenient to concentrate and recycle. The organic acid calcium salt is easy to absorb and low in acid-binding capacity, and can replace partial stone powder to be added into the feed, so that the acid-binding capacity of the feed can be effectively reduced, and the growth performance of animals can be improved.

In order to solve the problem of recycling of marigold fermentation liquor, the application provides a method for treating marigold fermentation liquor, and the composition of organic acid calcium and amino acid obtained after treatment by the method can be used as a feed additive and has an obvious growth promoting effect on cultured animals.

Disclosure of Invention

In order to solve the problem of treatment of marigold fermentation water, the invention provides a feed additive composition containing calcium lactate, calcium acetate and valine and prepared by taking marigold fermentation liquor as a raw material and a preparation method thereof. By adding exogenous calcium lactate, calcium acetate and valine, the proportion of the calcium lactate, the calcium acetate and the valine is adjusted, the synergistic effect is obvious, and the growth promoting effect is obvious when the calcium lactate, the calcium acetate and the valine are added into feed.

The invention relates to a feed additive prepared from marigold fermentation liquor, which comprises organic acid calcium and amino acid.

Preferably, the organic acid calcium is calcium lactate and/or calcium acetate.

Preferably, the amino acid is valine.

Preferably, the feed additive prepared from marigold fermentation liquor comprises calcium lactate, calcium acetate and valine.

Preferably, in the feed additive prepared from the marigold fermentation liquor, the weight ratio of calcium lactate to calcium acetate is 3:1-1:3, and the ratio of calcium lactate to valine is 200:1-30: 1.

Preferably, the feed additive prepared from marigold fermentation liquor further comprises one or more components of a carrier and an emulsifier.

Preferably, the feed additive prepared from marigold fermentation liquor is in the form of powder, liquid or microcapsule.

On the other hand, the invention also relates to a method for preparing the feed additive by the marigold fermentation liquor, which comprises the steps of taking the marigold fermentation liquor as a raw material, adding calcium hydroxide or calcium oxide, adjusting the pH value to 7-9, concentrating and dehydrating.

The preparation method of the marigold fermentation liquor comprises the steps of adding lactic acid bacteria into fresh marigold flowers, and fermenting to obtain the marigold fermentation liquor.

Preferably, in order to obtain the feed additive with better growth promoting effect, exogenous lactic acid, acetic acid and valine are added into the feed additive prepared from the marigold fermentation liquor to adjust the weight ratio of calcium lactate, calcium acetate and valine in the feed additive.

Preferably, exogenous lactic acid, acetic acid and valine are added to ensure that the weight ratio of calcium lactate to calcium acetate is 3:1-1:3 and the weight ratio of calcium lactate to valine is 200:1-30:1 in the feed additive.

Preferably, the method for preparing the feed additive from the marigold fermentation broth further comprises preparing the feed additive in a powder, liquid or microcapsule form.

In another aspect, the present invention is directed to a method of treating a marigold fermentation broth comprising the steps of:

step (1), adding calcium hydroxide or calcium oxide into marigold fermentation liquor, adjusting the pH value to 7-9, concentrating and dehydrating;

and (2) optionally, adding a feed additive into the concentrated and dehydrated composition.

Preferably, the feed additive is one or a mixture of more than two of lactic acid, acetic acid and valine.

Preferably, the weight ratio of calcium lactate to calcium acetate in the composition obtained in step (1) is 3:1-1:3, and the ratio of calcium lactate to valine is 200:1-30:1 by adding feed additives.

Preferably, the method for treating marigold fermentation liquor comprises the step (3) of preparing the composition obtained in the step (1) or the step (2) into a powder, liquid or microcapsule form.

The invention has the beneficial effects that:

(1) the method effectively realizes the reutilization of the marigold fermentation water, and solves the problems of difficult treatment and high cost of the marigold fermentation water in the prior art. The feed additive prepared from marigold fermented water can be used in feed.

(2) By adjusting the weight ratio of calcium lactate to calcium acetate in the feed additive to 3:1-1:3 and the weight ratio of calcium lactate to valine to 200:1-30:1, a good technical effect of promoting animal growth can be obtained. Under the condition of the same additive amount, the growth promoting effect is better than that of the three components which are used independently.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

EXAMPLE 1 preparation of Tagetes Erecta fermentation liquid extract

100 tons of fresh marigold flowers are added with 20 g/ton of lactobacillus (the effective viable count is 100 hundred million cfu/g, the fermentation temperature is 35 ℃, the fermentation time is 5 days, 60.8 tons of fermentation liquor is obtained, the pH value of the fermentation liquor is adjusted to 8.0 by calcium hydroxide, and 3.6 tons of pasty composition is obtained after concentration and dehydration, wherein the composition contains 40 percent of calcium lactate, 8 percent of calcium acetate and 0.2 percent of valine.

EXAMPLE 2 preparation of marigold fermentation liquid extract

100 tons of fresh marigold flowers are added with 10 g/ton of lactobacillus (the effective viable count is 150 hundred million cfu/g, sealed anaerobic fermentation is carried out, the fermentation temperature is 33 ℃, the fermentation time is 7 days, 63.2 tons of fermentation liquor are obtained, the pH value of the fermentation liquor is adjusted to 7.0 by calcium hydroxide, and 3.5 tons of pasty composition is obtained after concentration and dehydration, wherein the composition contains 38 percent of calcium lactate, 7 percent of calcium acetate and 0.1 percent of valine.

EXAMPLE 3 preparation of marigold fermentation liquid extract

100 tons of fresh marigold flowers are added with 15 g/ton of lactobacillus (the effective viable count is 125 hundred million cfu/g, the fermentation temperature is 30 ℃, the fermentation time is 7 days, 61.0 tons of fermentation liquor is obtained, the pH value of the fermentation liquor is adjusted to 9.0 by calcium hydroxide, and 3.5 tons of pasty composition is obtained after concentration and dehydration, wherein the composition contains 39 percent of calcium lactate, 6 percent of calcium acetate and 0.2 percent of valine.

Example 4 preparation of feed additive from marigold fermentation broth extract

50kg of marigold fermentation broth extract (obtained from example 1) containing 20kg of calcium lactate, 4kg of calcium acetate and 0.1kg of valine, and 16kg of exogenous calcium acetate and 0.17kg of valine were added to obtain a composition containing 20kg of calcium lactate, 20kg of calcium acetate and 0.27kg of valine. The ratio of calcium lactate to calcium acetate in the composition is 1:1 and the ratio of calcium lactate to valine is 74: 1.

Example 5 preparation of feed additive from Tagetes Erecta fermentation broth

100kg of marigold fermentation broth extract (obtained from example 2) containing 38kg of calcium lactate, 7kg of calcium acetate and 0.1kg of valine. To the cream composition, 2kg of calcium lactate, 6.33kg of calcium acetate, and 0.1kg of valine were further added, and the obtained composition contained 40kg of calcium lactate, 13.33kg of calcium acetate, and 0.2kg of valine. The ratio of calcium lactate to calcium acetate in the composition is 3:1, and the ratio of calcium lactate to valine in the composition is 200: 1.

Example 6 preparation of feed additive from Tagetes Erecta fermentation broth

100kg of marigold fermentation broth extract (from example 3) containing 39kg of calcium lactate, 6kg of calcium acetate and 0.2kg of valine. To the cream composition, 1kg of calcium lactate, 114kg of calcium acetate, and 1.13kg of valine were further added, and the obtained composition contained 40kg of calcium lactate, 120kg of calcium acetate, and 1.33kg of valine. The ratio of calcium lactate to calcium acetate in the composition is 1:3, and the ratio of calcium lactate to valine in the composition is 30: 1.

Comparative example 1

Analytically pure calcium lactate with a content of 99% or more, purchased from Mimi European chemical reagent Co., Ltd, Tianjin.

Comparative example 2

Analytically pure calcium acetate, with a content of 98% or more, was purchased from Fochen chemical reagents, Inc.

Comparative example 3

Food grade valine with content of 99.5% or more, which is purchased from Hebei Huayang Biotech limited company

Comparative example 4

The feed additive composition is prepared by compounding calcium lactate, calcium acetate and valine, wherein 50kg of calcium lactate, 5kg of calcium acetate and 0.5kg of valine are added, the ratio of the calcium lactate to the calcium acetate is 10:1, and the ratio of the calcium lactate to the valine is 100: 1.

Comparative example 5

The feed additive composition is prepared by compounding calcium lactate, calcium acetate and valine, wherein 10kg of calcium lactate, 50kg of calcium acetate and 5kg of valine are added, the ratio of the calcium lactate to the calcium acetate is 1:5, and the ratio of the calcium lactate to the valine is 2: 1.

Example 7 evaluation of growth promoting Effect

Experimental animals and experimental design: the experimental animals are 324 white feather broilers with the age of 1 day, the experiment adopts single-factor design, the experimental animals are divided into 9 treatment groups in total, each treatment group has 6 repetitions, and each repetition has 6 chickens. Treatment 1 was the basal diet group, and the other 6(8) treatment groups were the products of example 1, example 2, and example 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, and comparative example 5, respectively, to which 0.5g/kg (based on the total content of calcium lactate, calcium acetate, and valine) was added to the basal diet.

And (3) experimental diet: the daily ration of the tested chicken is prepared by taking corn soybean meal type daily ration as basic daily ration according to the nutrition requirements of broiler chicken breeding standard (NY/T33-2004) of agricultural industry standard of the people's republic of China, and the specific formula is shown in Table 1.

Table 1 basic ration composition (%)

Feeding management: all the experimental broilers are raised in cages, 6 chickens are raised in each cage, 6 processed chickens are evenly distributed in the laboratory, and the chickens are evenly distributed in the upper, middle and lower layers. The breeding management refers to a positive white feather broiler breeding management manual.

Measurement indexes are as follows: daily feed intake, daily gain, and calculating the feed-weight ratio (daily feed intake/daily gain).

And (3) test results:

TABLE 2 Effect of different additive combinations in the daily ration on the growth performance of broiler (unit: g)

Item	Average daily food intake	Average daily gain	Material to weight ratio
				Basal diet group	87.32	52.92	1.65
EXAMPLE 1 group	88.54	59.42	1.49
				EXAMPLE 2 group	87.97	58.65	1.50
EXAMPLE 3 group	88.01	59.47	1.48
				Comparative example 1 group	87.56	55.77	1.57
Comparative example 2 group	87.45	54.66	1.60
				Comparative example 3 group	87.86	53.57	1.64
Comparative example 4 group	87.98	56.40	1.56
				Comparative example 5 group	88.03	56.72	1.55

As can be seen from table 2 above, the feed weight ratios of the comparative examples 1-3 groups were slightly lower than the feed medium ratio of the basal diet group. The feed using the feed additive of the present application, which had a lower feed weight ratio in the groups of examples 1-3, demonstrated that the feed additive of the present application had a better growth promoting effect than the three components used alone at the same additive level.

While embodiments of the present invention have been described, the present invention is not limited to the above-described embodiments, which are intended to be illustrative rather than limiting, and many modifications may be made by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.