阅读说明：本技术 一种纳米药发生装置及用途 (Nano medicine generating device and application ) 是由 唐峰 石蕾 胡军 于 2021-06-18 设计创作，主要内容包括：本发明公开了一种纳米药发生装置及用途,包括高压电源系统、电极对、工作介质、过滤层、吸附层和供给部件。所述高压电源系统可为高压电源或者变压器,用以提供高压电场；所述电极对分别与高压电源系统的输出端的两侧相电连接,用以加载高压电场。所述工作介质在高压电场的作用下,经历电子雪崩过程放电,以大面积地获取粒径为0-1000纳米的超细粒子、含氮自由基和含氧自由基中的至少一种纳米活性物质。该装置还设有吸附层或过滤层,以提高纳米药的纯度,并根据不同的用途,智能调节纳米活性物质的组分和浓度,可用于皮肤病及伤口护理、美容抗衰、防脱发及生发、口腔护理、呼吸道疾病防治等医疗器械。(The invention discloses a nano-drug generating device and application thereof, comprising a high-voltage power supply system, an electrode pair, a working medium, a filter layer, an adsorption layer and a supply component. The high-voltage power supply system can be a high-voltage power supply or a transformer and is used for providing a high-voltage electric field; the electrode pairs are respectively and electrically connected with two sides of the output end of the high-voltage power supply system and used for loading a high-voltage electric field. The working medium is discharged in an electron avalanche process under the action of a high-voltage electric field, so that at least one nano active substance of superfine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals is obtained in a large area. The device is also provided with an adsorption layer or a filter layer to improve the purity of the nano-drug, intelligently adjust the components and the concentration of the nano-active substances according to different purposes, and can be used as medical instruments for skin disease and wound care, beauty treatment and anti-aging, hair loss prevention, hair growth, oral care, respiratory disease prevention and treatment and the like.)

1. The utility model provides a nanometer medicine generating device, includes high voltage power supply system and electrode pair, the electrode pair includes electrode I and electrode II, one side electricity of high voltage power supply system's output is connected the tip of electrode I, the opposite side electricity of high voltage power supply system's output is connected the tip of electrode II, its characterized in that: the electrode I and the electrode II are oppositely arranged and are not in contact with each other;

the electrode I and the electrode II are used for loading a high-voltage electric field under the action of a high-voltage power supply system;

the nano-drug generating device also comprises at least one working medium, and the working medium is subjected to electron avalanche process discharge under the action of a high-voltage electric field between the electrode I and the electrode II so as to obtain at least one nano active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals.

2. The nano-drug generating device according to claim 1, wherein: the nano-drug generating device also comprises a blocking medium, wherein the blocking medium is a medium with high dielectric constant, and is arranged between the electrode pair to prevent the electrode pair from discharging outside a working medium region, stabilize a high-voltage electric field and generate uniform ionization.

3. A nano-drug generating device according to claim 1 or 2, wherein: the nano-drug generating device further comprises an adsorption layer, wherein the adsorption layer is arranged at the downstream of the electrode pair and used for adsorbing byproducts in the nano-active substances.

4. The nano-drug generating device according to claim 3, wherein: the adsorption layer comprises at least one of calcium hydroxide, potassium hydroxide and sodium hydroxide and is used for adsorbing nitrogen dioxide and ozone.

5. The nano-drug generating device according to claim 3, wherein: the nano-drug generating device also comprises a filter layer which is arranged at the downstream of the electrode pair and used for filtering particles emitted by the electrode pair, particles with the particle size of more than 10 microns or other impurities entering from the outside.

6. The nano-drug generating device according to claim 5, wherein: the filter layer is provided at least one of upstream and downstream of the adsorption layer.

7. A nano-drug generating device according to claim 1 or 2, wherein: the nano-drug generating device further comprises a supply component for providing a working medium and providing power for the flow of the working medium to cool the nano-drug generating device and maintain an acceptable low temperature level.

8. The nano-drug generator according to claim 7, wherein: the supply component is also provided with a component concentration or flow sensor to maintain the proper component and dosage of the working medium.

9. A nano-drug generating device according to claim 1 or 2, wherein: the nano-drug generating device further comprises an intelligent sensor, wherein the intelligent sensor is used for detecting the concentration of at least one nano active substance in the ultra-fine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals, and intelligently adjusting the working parameters of the high-voltage power supply system, and the components and the dosage of the working medium.

10. A nano-drug generating device according to claim 1 or 2, wherein: the nano active substance is low-temperature plasma with electron density of 10⁷-10²³/cm³。

11. A nano-drug generating device according to claim 1 or 2, wherein: the working medium is one or more of hyaluronic acid, collagen, water vapor, hydrogen, methane, oxygen, nitrogen, carbon dioxide, air, rare gas, essential oil, physiological saline or medicine.

12. A nano-drug generating device according to claim 1 or 2, wherein: the electrode pairs are electrically connected by a partially ionized operating medium or a broken down blocking medium during an electron avalanche process.

13. A nano-drug generating device according to claim 1 or 2, wherein: the high-voltage power supply system is a high-voltage power supply or a transformer, and the output high voltage of the high-voltage power supply system is direct-current high voltage or alternating-current high voltage.

14. A nano-drug generating device according to claim 1 or 2, wherein: the output high voltage of the high-voltage power supply system is pulse high voltage.

15. Use of a nano-drug generating device to generate nano-active substances for alleviating skin disorders, characterized by: the nano-drug generating device provides a working medium to generate at least one nano-active substance of ultra-fine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals through electron avalanche process discharge, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier acts on the skin to increase the oxygenation effect and hemoglobin index of the skin, inhibit the activation of mast cells and eosinophilic granulocyte, inhibit the expression and generation of IL-6 and TNF-alpha, inhibit the activation of NF-kappa B and improve allergic dermatitis.

16. Use of a nano-drug generating device to generate nano-actives for oral care, characterized by: the nano active substance or the nano medicine taking the nano active substance as a carrier acts on gingiva, periodontal or implant, improves the surface hydrophilicity of the nano active substance, inhibits bacteria and reduces inflammation, relieves periodontitis, promotes the growth of osteoblasts or histiocytes, improves wound healing and improves the success rate of the implant.

17. The application of the nano active substance generated by the nano drug generating device for relieving the respiratory diseases is characterized in that: the nano-drug generating device provides a working medium to generate at least one nano-active substance of ultrafine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge of an electron avalanche process, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier acts on respiratory tracts, improves the oxygenation of tissues, promotes the vasodilatation, improves the level of inflammatory media and promotes the regeneration of damaged tissue cells.

18. The utility model provides an application of nanometer active material suppression tumor cell is produced to nanometer medicine generating device which characterized in that: the nano active substance or nano medicine taking the nano active substance as a carrier acts on tumor cells to activate macrophages, T cells or NK cells, induce the immunogenic cell death of the tumor cells, systematically trigger specific and protective immune response, polarize cell membranes, cause oxidative stress reaction and induce the iron death of the tumor cells.

19. The application of the nanometer active substance generated by the nanometer medicine generating device for whitening and resisting aging is characterized in that: the nano active substance or the nano medicine taking the nano active substance as a carrier acts on the skin to increase the oxygenation effect and tissue perfusion of the skin, remove surface photoaging epidermal cells, promote the growth of collagen of the skin below, activate vascular endothelial growth factors, and promote the expression of anti-aging genes and tissue regeneration.

20. Use of a nanomedicine generating device to produce a nanoactive substance for hair growth, characterized by: the nano-drug generating device provides a working medium to generate at least one nano-active substance of ultrafine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge of an electron avalanche process, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier acts on the scalp to promote the production of adenosine triphosphate, activate the DNA transcription factor of NF-kappa B, HIF-1, promote the cell proliferation, improve the levels of cytokine, growth factor and inflammatory medium, improve the tissue oxygenation and promote the hair growth.

Technical Field

The invention relates to the field of medical equipment and health care theory, in particular to a nano-drug generating device and application.

Background

The traditional medicine is generally administered by intravenous injection or oral administration. However, the traditional drug therapy is expensive, low in drug utilization rate, extremely limited in therapeutic effect and large in systemic side effect.

Due to its high activity, high permeability and low side effect, nano-drug is becoming a hot spot of current medical research as a new drug delivery mode or drug carrier.

In view of the above, the present invention provides a nano-drug generating device and its use.

Disclosure of Invention

The invention aims to provide a nano-drug generating device and application aiming at the defects of the prior art.

In order to solve the technical problems, the following technical scheme is adopted:

a nanometer medicine generating device comprises a high-voltage power supply system and an electrode pair, wherein the electrode pair comprises an electrode I and an electrode II, one side of the output end of the high-voltage power supply system is electrically connected with the end part of the electrode I, the other side of the output end of the high-voltage power supply system is electrically connected with the end part of the electrode II, and the nanometer medicine generating device is characterized in that: the electrode I and the electrode II are oppositely arranged and are not in contact with each other;

the electrode I and the electrode II are used for loading a high-voltage electric field under the action of a high-voltage power supply system;

the nano-drug generating device also comprises at least one working medium, and the working medium is subjected to electron avalanche process discharge under the action of a high-voltage electric field between the electrode I and the electrode II so as to obtain at least one nano active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals.

Furthermore, the nano-drug generating device also comprises a blocking medium, wherein the blocking medium is a medium with high dielectric constant, and is arranged between the electrode pair to prevent the electrode pair from discharging outside the working medium region, stabilize a high-voltage electric field and generate uniform ionization.

Furthermore, the nano-drug generating device also comprises an adsorption layer, wherein the adsorption layer is arranged at the downstream of the electrode pair and is used for adsorbing byproducts in the nano-active substances.

Further, the adsorption layer comprises at least one of calcium hydroxide, potassium hydroxide and sodium hydroxide and is used for adsorbing nitrogen dioxide and ozone.

Furthermore, the nano-drug generating device also comprises a filter layer which is arranged at the downstream of the electrode pair and used for filtering particles emitted by the electrode pair, particles with the particle size of more than 10 microns or other impurities entering from the outside.

Further, the filter layer is provided at least one of upstream and downstream of the adsorption layer.

Further, the nano-drug generating device further comprises a supply component for providing a working medium and providing power for the flow of the working medium to cool the nano-drug generating device and maintain an acceptable low temperature level.

Further, a component concentration or flow sensor is arranged on the supply component to maintain proper components and dosage of the working medium.

Further, the nano-drug generating device further comprises an intelligent sensor, wherein the intelligent sensor is used for detecting the concentration of at least one nano active substance in the ultra-fine particles, the nitrogen-containing free radicals and the oxygen-containing free radicals with the particle size of 0-1000 nanometers, and intelligently adjusting the working parameters of the high-voltage power supply system, and the components and the dosage of the working medium.

Further, the nano active substance is low-temperature plasma with electron density of 10⁷-10²³/cm³。

Further, the working medium is one or more of hyaluronic acid, collagen, water vapor, hydrogen, methane, oxygen, nitrogen, carbon dioxide, air, rare gas, essential oil, physiological saline or medicine.

Further, the electrode pairs are electrically connected through a partially ionized operating medium or a broken down blocking medium during the electron avalanche process.

Further, the high-voltage power supply system is a high-voltage power supply or a transformer, and the output high voltage of the high-voltage power supply system is direct-current high voltage or alternating-current high voltage.

Further, the output high voltage of the high-voltage power supply system is pulse high voltage.

According to another technical scheme, the nano-drug generating device is used for generating nano-active substances for relieving skin diseases, a working medium is provided through the nano-drug generating device and is subjected to electron avalanche process discharge to generate at least one nano-active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals, and the nano-active substances or the nano-drugs taking the nano-active substances as carriers act on the skin to increase the oxygenation effect and hemoglobin index of the skin, inhibit the activation of mast cells and eosinophilic granulocytes, inhibit the expression and generation of IL-6 and TNF-alpha, inhibit the activation of NF-kappa B and improve allergic dermatitis.

According to another technical scheme, the nano-drug generating device is used for generating nano-active substances for oral care, the nano-drug generating device is used for providing a working medium to generate at least one nano-active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through discharge in an electron avalanche process, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier is used for gingiva, periodontium or an implant to improve the surface hydrophilicity of the nano-active substance, inhibit bacteria and diminish inflammation, relieve periodontitis, promote the growth of osteoblasts or histiocytes, improve wound healing and improve the success rate of the implant.

According to another technical scheme, the nano-drug generating device is used for generating nano-active substances for relieving respiratory diseases, working media are provided by the nano-drug generating device to generate at least one nano-active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through electron avalanche process discharge, and the nano-active substances or the nano-drugs taking the nano-active substances as carriers act on respiratory tracts, so that tissue oxygenation is improved, vasodilatation is promoted, the level of inflammatory media is improved, and regeneration of damaged tissue cells is promoted.

According to another technical scheme, the nano-drug generating device is used for generating nano-active substances to inhibit tumor cells, the nano-drug generating device is used for providing a working medium to generate at least one nano-active substance of superfine particles, nitrogen-containing free radicals and oxygen-containing free radicals with the particle size of 0-1000 nanometers through electron avalanche process discharge, the nano-active substance or the nano-drug taking the nano-active substance as a carrier is used for the tumor cells, macrophages, T cells or NK cells are activated, immunogenic cell death of the tumor cells is induced, specific and protective immune response is systematically triggered, cell membranes are polarized, oxidation stress reaction is caused, and iron death of the tumor cells is induced.

The other technical scheme of the invention is that a nano-drug generating device generates nano-active substances for whitening and anti-aging, a working medium is provided by the nano-drug generating device to generate at least one nano-active substance of superfine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge in the electron avalanche process, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier is applied to the skin to increase the oxygenation and tissue perfusion of the skin, remove surface photo-aged epidermal cells, promote the growth of underlying skin collagen, activate vascular endothelial growth factors and promote the expression of anti-aging genes and tissue regeneration.

The other technical scheme of the invention is that a nano-drug generating device generates nano-active substances for hair growth, working media provided by the nano-drug generating device are discharged in an electron avalanche process to generate at least one nano-active substance of superfine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals, and the nano-active substances or nano-drugs taking the nano-active substances as carriers are applied to the scalp to promote the production of adenosine triphosphate, activate DNA transcription factors of NF-kappa B, HIF-1, promote cell proliferation, improve the levels of cytokines, growth factors and inflammatory media, improve tissue oxygenation and promote hair growth.

Due to the adoption of the technical scheme, the method has the following beneficial effects:

the invention relates to a nano-drug generating device and application, wherein a working medium is subjected to electron avalanche process discharge under the action of a high-voltage electric field between an electrode pair to obtain at least one nano active substance of superfine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals, an adsorption layer or a filter layer is further arranged to improve the purity of the nano-drug, and the components and the concentration of the nano active substance are intelligently adjusted according to different applications, so that the nano-drug generating device can be used for medical instruments such as skin disease and wound care, beauty and anti-aging, alopecia prevention and hair growth, oral care, respiratory disease prevention and treatment and the like.

The nanomedicine generating device is further provided with a blocking medium, preferably a high dielectric constant medium, disposed between the pair of electrodes for stabilizing the high voltage electric field and producing uniform ionization.

The nano-drug generating device is also provided with an adsorption layer or a filter layer for improving the purity of the nano-drug.

The nano-drug generating device is also provided with a supply component for providing a working medium and providing power for the flow of the working medium to cool the nano-drug generating device and maintain an acceptable low temperature level.

The supply component is also provided with a component concentration or flow sensor to maintain the proper component and dosage of the working medium.

The nano-drug generating device also comprises an intelligent sensor, wherein the intelligent sensor is used for detecting the concentration of at least one nano active substance in the ultra-fine particles with the particle size of 0-1000 nm, nitrogen-containing free radicals and oxygen-containing free radicals, and intelligently adjusting the working parameters of the transformer and the components and the dosage of the working medium, so that the components and the concentration of the nano active substance are adjusted, and different purposes and requirements are met.

The nano active substance is low-temperature plasma with electron density of 10⁷-10²³/cm³。

The output high voltage of the high-voltage power supply system is preferably pulse high voltage, so that the working temperature is reduced and the biocompatibility is improved while the nano-drug is efficiently manufactured. In addition, the nano-drug generating device can continuously work for a long time, is stable in work, safe, effective, high in purity and small in size, and can be applied to various medical instruments and health care.

Drawings

The invention will be further described with reference to the accompanying drawings in which:

fig. 1 is a schematic structural diagram of a nano-drug generating device according to a first embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a nano-drug generating device according to a second embodiment of the present invention;

FIG. 3 is a graph showing the concentration of ultrafine particles having a partial particle diameter (11.5-64.9 nm) generated by the nano-drug generating device according to the present invention;

FIG. 4 is a graph of the spectrum of hydroxyl radicals generated by the nanomedicine generating device according to the present invention (ESR tester);

FIG. 5 is a graph of NO concentration produced by a nanomedicine generating device according to the present invention;

FIG. 6-1 is a graph of the stratum corneum of M2-type macrophages before treatment;

FIG. 6-2 is a graph of the stratum corneum of M2-type macrophages after treatment;

FIGS. 6-3 are glandular views of M2-type macrophages before treatment;

FIGS. 6-4 are glandular views of M2-type macrophages after treatment;

FIG. 7-1 is a graph showing the improvement of the surface treatment effect of PEKK implant by sulfonation and titanium dioxide;

FIG. 7-2 is a diagram illustrating the improvement of the surface treatment effect of the PEKK implant surface by adding the nano-active substance and titanium dioxide through sulfonation;

FIG. 7-3 is a graph showing the improvement of the surface treatment effect of PEKK implant surface by adding nano-active material, titanium dioxide and SBF;

FIG. 8 is a graph showing the effect of inhibiting fibroblast proliferation;

FIG. 9 is a graph showing the effect of inhibiting tumor cells;

FIG. 10 is a number bar graph after tumor cell inhibition;

FIG. 11 is a graph showing the effect of inhibiting melanocytes;

FIG. 12-1 is a flow cytogram A of melanocyte inhibition;

FIG. 12-2 is a flow cytogram B of melanocyte inhibition;

FIGS. 12-3 are flow cytograms C for the inhibition of melanocytes;

FIGS. 12-4 are flow cytograms D for the inhibition of melanocytes;

fig. 13 is a graph showing the effect of promoting hair growth.

In the figure: 1. the high-voltage power supply system comprises a high-voltage power supply system 11, transformer primary sides I and 12, transformer primary sides II and 13, transformer secondary sides I and 14, transformer secondary sides II and 2, an electrode pair 2A, electrodes I and 2B, electrodes II and 3, a blocking medium 4, a working medium 5, an intelligent sensor 6, a supply part 7, a filter layer 8, an adsorption layer 9, a nanometer active substance release port 10 and a component concentration or flow sensor.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and examples. It should be understood, however, that the description herein of specific embodiments is only intended to illustrate the invention and not to limit the scope of the invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.

Example 1

Referring to fig. 1, a nano-drug generating device includes main components: high-voltage power supply system 1, electrode pair 2, working medium 4, filter layer 7, adsorption layer 8 and supply unit 6.

The electrode pair 2 comprises an electrode I2A and an electrode II 2B, one side of the output end of the high-voltage power supply system 1 is electrically connected with the end part of the electrode I2A, the other side of the output end of the high-voltage power supply system 1 is electrically connected with the end part of the electrode II 2B, and the electrode I2A and the electrode II 2B are oppositely arranged and are not in contact with each other; and the electrode I2A and the electrode II 2B are used for loading a high-voltage electric field under the action of the high-voltage power supply system 1.

As a further supplement to this embodiment, the high voltage power supply system 1 is a high voltage power supply or a transformer, and the output high voltage of the high voltage power supply system 1 is a direct current or an alternating current high voltage. When the high-voltage power supply system 1 is a transformer, referring to fig. 1, one side of the transformer is a low-voltage input end, the low-voltage input end is a primary side i 11 of the transformer and a primary side ii 12 of the transformer, the other side of the transformer is a high-voltage output end, the high-voltage output end is a secondary side i 13 of the transformer and a secondary side ii 14 of the transformer, and the electrode i 2A and the electrode ii 2B are electrically connected with the secondary side i 13 of the transformer and the secondary side ii 14 of the transformer respectively. The electrode I2A and the electrode II 2B are used for loading a high-voltage electric field under the action of a high-voltage power supply system 11.

As a further supplement to this embodiment, the working medium 4 undergoes electron avalanche process discharge under the action of the high voltage electric field between the electrode i 2A and the electrode ii 2B, thereby obtaining at least one nano active material of ultra-fine particles with a particle size of 0 to 1000 nm, nitrogen-containing radicals and oxygen-containing radicals.

As a further supplement to this embodiment, the adsorption layer 8 is disposed downstream of the electrode pair 2 for adsorbing by-products in the nano-active material.

The adsorption layer 8 is preferably composed of at least one of calcium hydroxide, potassium hydroxide and sodium hydroxide, and is used for adsorbing byproducts such as nitrogen dioxide and ozone, so as to improve the purity of the nano-drug.

As a further supplement to this embodiment, the filter layer 7 is disposed downstream of the electrode pair 2, and is used for filtering particles emitted from the electrode pair 2, particles with a particle size of 10 microns or more, or other impurities entering from the outside, preventing allergy, and improving the purity of the nano-drug.

As a further complement to this embodiment, the filter layer 7 is provided at least one of upstream and downstream of the adsorbent layer 8.

As a further supplement to this embodiment, the blocking medium is a high dielectric constant medium, and is disposed between the electrode pair 2 to prevent the electrode pair 2 from discharging outside the region of the working medium 4, and to stabilize the high voltage electric field and generate uniform ionization.

The electrode pair of the device is different from the electrode pair of the prior art, the electrode pair of the prior art is generally placed in a relatively closed environment, and the electrode pair of the device can be placed in an open environment or a relatively closed environment to achieve the same effect.

As a further supplement to this embodiment, the nano-drug generating apparatus further comprises an intelligent sensor 5, and the intelligent sensor 5 is configured to detect the concentration of at least one nano-active substance of the ultra-fine particles, the nitrogen-containing radicals and the oxygen-containing radicals having a particle size of 0 to 1000 nm, and intelligently adjust the operating parameters of the high-voltage power supply system 1, the composition and the dosage of the operating medium 4.

As a further complement to this embodiment, the nanomedicine generator further comprises a supply means 6 for providing the working medium 4 and powering the flow of the working medium 4 to cool the nanomedicine generator to maintain an acceptably low temperature level.

As a further complement to this embodiment, a component concentration or flow sensor 10 is provided on the supply member 6 to maintain the proper component and dosage of the working medium 4.

As a further supplement to this example, the nano-active substance was a low temperature plasma with an electron density of 10⁷-10²³/cm³。

The tail part of the nano-drug generating device is provided with a nano-active substance release port 9.

As a further supplement to this embodiment, the working medium 4 is one or more of hyaluronic acid, collagen, water vapor, hydrogen, methane, oxygen, nitrogen, carbon dioxide, air, noble gas, essential oil, physiological saline, or medicine.

As a further complement to this embodiment, the electrode pair 2 is electrically connected through the partially ionized operating medium 4 or the broken down blocking medium during the electron avalanche process.

As a further supplement to this embodiment, the blocking medium and the working medium 4 may be the same medium, such as air, in which case, a part of the working medium 4 is ionized by discharging in an electron avalanche process, and another part of the working medium 4 is not ionized and is in a dielectric state, and is used as the blocking medium.

The blocking medium and the working medium 4 may be different mediums, and in this case, the blocking medium may be disposed on the periphery of any one of the electrodes in the electrode pair 2, or disposed between the electrode pair 2. In this embodiment, the electrode pair 2 penetrates through the blocking medium, and the blocking medium also plays a role in fixing the electrode pair 2, so as to further ensure the stability of the electric field.

The electrode pair 2 is any combination of needle shape, rod shape, strip shape, sheet shape, ring shape, etc. In the present embodiment, the electrode pairs 2 are all configured in a needle shape to maintain the local accumulation of the high voltage electric field, so as to induce the electron avalanche process of the working medium 4 more easily to discharge.

As a further supplement to this embodiment, the output high voltage of the high voltage power supply system 1 is a pulsed high voltage. The nano-drug is efficiently produced, and simultaneously, the working temperature is reduced and the biocompatibility is improved.

As a further supplement of this embodiment, the nano active substance can be directly used as a nano drug, and can be used as a carrier to be added with other drugs to form a new nano drug for medical purposes, such as skin disease and wound care, beauty treatment and anti-aging, alopecia prevention and hair growth, oral care, respiratory disease prevention and treatment, tumor treatment, and the like.

Example 2.

As a modification to embodiment 1, as shown in fig. 2, one electrode i 2A of the electrode pair 2 is needle-shaped, the other electrode ii 2B is ring-shaped, and the electrode ii 2B is disposed on the circumference of the blocking medium. The blocking medium is also in a circular ring shape and is arranged between the electrode pair 2, so that uniform ionization can be ensured, the electrode II 2B can be prevented from contacting with the working medium 4, the electrode II 2B can be prevented from being corroded when the working medium 4 discharges, and the discharging stability can be further ensured. Other features are the same as in the first embodiment.

Specific experimental data are shown in table 1:

TABLE 1 Experimental data sheet of nano-drug generator (ambient temperature 25 deg.C, relative humidity 55%)

As can be seen from Table 1 and FIGS. 3-5: the nano-drug generator of the present embodiment produces a large amount of nano-active materials such as ultra-fine particles having a particle size of 0 to 1000 nm, nitrogen-containing radicals and oxygen-containing radicals, and can generate a large amount of oxygen-containing radicals (e.g., hydroxyl radicals) and nitrogen-containing radicals (e.g., nitrogen monoxide) having a concentration of 15 to 27ppm or more, and can be used for medical purposes. In addition, under the same conditions, the embodiment adopts pulse high voltage, so that the temperature of the working medium 4 can be reduced and the biocompatibility of the working medium can be improved while the nano-drug is efficiently manufactured.

Example 3

The nano-drug generating device is used for providing a working medium 4 to generate at least one nano-active substance of ultrafine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through discharging in an electron avalanche process, and the nano-active substance or the nano-drug taking the nano-active substance as a carrier acts on the skin to increase the oxygenation effect and hemoglobin index of the skin, inhibit the activation of mast cells and eosinophilic granulocytes, inhibit the expression and generation of IL-6 and TNF-alpha, inhibit the activation of NF-kappa B and improve allergic dermatitis.

In this embodiment, allergic eczema is taken as an example. Referring to fig. 6-1, before treatment, M2 type macrophages are significantly soaked, the stratum corneum is relatively thick, and after treatment with the nano active substance, the stratum corneum becomes thin and is significantly seen in fig. 6-2. With reference to fig. 6-3, the macrophages of type M2 enlarged glands before treatment, and with reference to fig. 6-4, the macrophages of type M2 became significantly smaller glands after treatment. Therefore, after the nanometer active substance acts on the skin, the activation of mast cells and eosinophils is inhibited, the stratum corneum is thinned and recovered to be normal, the parakeratosis is inhibited, and the allergic eczema is improved.

Example 4

A nanometer medicine generating device generates nanometer active substances for oral care, a working medium 4 is provided by the nanometer medicine generating device to generate at least one nanometer active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge of an electron avalanche process, and the nanometer active substances or the nanometer medicine taking the nanometer active substances as a carrier act on gingiva, periodontium or an implant to improve the surface hydrophilicity, inhibit bacteria and diminish inflammation, relieve periodontitis, promote the growth of osteoblasts or histiocytes, improve wound healing and improve the success rate of the implant.

In this example, the nano-active substance acts on the implant, taking the PEKK implant as an example, and referring to fig. 7-1, 7-2 and 7-3, the density of these small particles of the PEKK implant is higher, which indicates that the more osteoblasts are present, which is more advantageous for the healing of the implant.

Referring to fig. 7-1, 7-2 and 7-3, sulfonation plus titanium dioxide surface treatment followed by deposition of hydroxyapatite in sbf demonstrates in vitro osteogenesis capacity. Experiments show that the effect of substituting the sulfonation step with the nano-drug is equivalent to the effect of sulfonation plus titanium dioxide surface treatment. If sulfonation is carried out first and then nano-drug treatment is carried out, the deposition of titanium dioxide can be promoted, the deposition of hydroxyapatite in sbf can be further better induced, the growth of osteoblasts or histiocytes can be promoted, the bacteria can be inhibited, the inflammation can be reduced, and the success rate of the implant can be improved.

Example 5

A nanometer medicine generating device generates nanometer active substances for relieving respiratory diseases, a working medium 4 is provided by the nanometer medicine generating device to generate at least one nanometer active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge in an electron avalanche process, and the nanometer active substances or the nanometer medicine taking the nanometer active substances as a carrier act on the respiratory tract, so that the oxygenation of tissues is improved, the vasodilatation is promoted, the level of inflammation media is improved, and the regeneration of damaged tissue cells is promoted.

In this example, the nano-active substance acts on the respiratory tract, taking lung fibrosis caused by interstitial pneumonia as an example, as shown in fig. 8, wherein the small dots represent fibroblasts, and the density of the small dots is higher, which indicates that the number of fibroblasts is more.

The nano medicine can improve tissue oxygenation, promote vasodilatation, improve the level of inflammatory mediators, remarkably inhibit fibroblast proliferation (the inhibition rate is up to more than 80 percent), and promote the regeneration of damaged tissue cells.

Example 6

A nanometer medicine generating device provides a working medium 4 to generate at least one nanometer active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge of an electron avalanche process, the nanometer active substance or the nanometer medicine taking the nanometer active substance as a carrier acts on tumor cells to activate macrophage cells, T cells or NK cells, induce the immunogenic cell death of the tumor cells, systematically trigger specific and protective immune responses, polarize cell membranes, cause oxidative stress reactions and induce the iron death of the tumor cells.

In this embodiment, the nano-active substance acts on the lung cancer cells, as shown in fig. 9, taking the lung cancer cells as an example, after 1 minute, 8 minutes and 16 minutes of action of the nano-drug, the inhibition rates of the lung cancer cells are respectively 50%, 70% and 100%, the lung cancer cells are completely inhibited, macrophages, T cells or NK cells are activated, immunogenic cell death of the tumor cells is induced, specific and protective immune responses are systematically triggered, and iron death of the tumor cells is induced.

FIG. 10 is a bar graph showing the number of viable cells after tumor cell inhibition. In particular, referring also to Table 2, the number of viable cells in the untreated control group was 282 × (10^6), and the tumor cells survived after 1 minute, 8 minutes and 16 minutes of the action of the nano-active substance were 141 × (10^6), 86 × (10^6) and 0.5 ^6, respectively, and the cancer cells were almost completely inhibited.

TABLE 2

Example 7

A nanometer medicine generating device provides a working medium 4 to generate at least one nanometer active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through discharging in an electron avalanche process, the nanometer active substance or the nanometer medicine taking the nanometer active substance as a carrier acts on the skin, the oxygen cooperation and tissue perfusion of the skin are increased, epidermal cells with surface light aging are removed, the growth of collagen of the skin below is promoted, vascular endothelial growth factors are activated, and the expression of anti-aging genes and tissue regeneration are promoted.

Referring to fig. 11, 12-1, 12-2, 12-3 and 12-4, taking B16 melanocytes as an example, the proliferation of melanocytes is significantly inhibited (the inhibition rate is more than 50%), and apoptosis is promoted, and after Hyaluronic Acid (HA) is added for combined treatment, apoptosis of melanocytes is further accelerated, which is beneficial for whitening, and expression of anti-aging genes and tissue regeneration are promoted.

In the present embodiment, the working medium 4 is preferably one or more of hyaluronic acid, collagen, and air.

Example 8

A nanometer medicine generating device provides a working medium 4 to generate at least one nanometer active substance of superfine particles with the particle size of 0-1000 nanometers, nitrogen-containing free radicals and oxygen-containing free radicals through the discharge of an electron avalanche process, the nanometer active substance or the nanometer medicine taking the nanometer active substance as a carrier acts on the scalp to promote the production of adenosine triphosphate, activate the DNA transcription factor of NF-kappa B, HIF-1, promote the cell proliferation, improve the levels of cytokine, growth factor and inflammatory medium, improve the tissue oxygenation and promote the hair growth.

Referring to FIG. 13 and Table 3, in the mouse experiment, using the nano-active substance for 8 weeks, minoxidil at 5% concentration for 8 weeks, and finasteride at 1 mg/day for 4 weeks, respectively, a statistically significant increase in the peripheral hair density, hair growth promotion, and overall improvement in the thickness and fullness of hair were observed, and the increase in hair in the nano-drug treatment group (8 weeks) was comparable to the test effect of minoxidil at 5% concentration (8 weeks) and finasteride at 1 mg/day (4 weeks).

TABLE 3

The above is only a specific embodiment of the present invention, but the technical features of the present invention are not limited thereto. Any simple changes, equivalent substitutions or modifications made on the basis of the present invention to solve the same technical problems and achieve the same technical effects are all covered in the protection scope of the present invention.