阅读说明：本技术 一种羊肝谷物重组食品 (Sheep liver cereal recombinant food ) 是由 陈敬 张超垒 于 2021-02-03 设计创作，主要内容包括：本发明涉及食品技术领域,具体涉及一种羊肝谷物重组食品,以羊肝、谷物和牛奶为主要原料制备而成,无菌条件下将牛奶粉、羊肝谷物压片、蔗糖混匀后包装制得羊肝谷物重组食品；其中羊肝谷物压片的制备：取米粉、小麦全麦粉、燕麦全麦粉、小米全营养液和大豆活性物质、羊肝匀浆及其细胞溶出液,加水搅拌混合均匀后恒温酶解得羊肝谷物制品；醒发得羊肝谷物面糊；置于的模具中,低温烘烤至面糊呈现面团状时压片,底火加热至压片呈现金黄色后取出冷却,粉碎得羊肝谷物压片；本发明产品含功能多肽、大豆异黄酮、膻味、口感独特、易于冲调饮用。(The invention relates to the technical field of food, in particular to a sheep liver cereal recombinant food which is prepared by taking sheep liver, cereal and milk as main raw materials, and the sheep liver cereal recombinant food is prepared by uniformly mixing and packaging milk powder, sheep liver cereal and cane sugar under an aseptic condition; wherein, the preparation of the sheep liver cereal tablet comprises the following steps: taking rice flour, whole wheat flour, whole oat flour, whole millet nutrient solution, soybean active substances, sheep liver homogenate and cell lysate thereof, adding water, stirring uniformly, and performing enzymolysis at constant temperature to obtain sheep liver cereal product; fermenting to obtain lamb liver cereal paste; placing in a mold, baking at low temperature until the paste is in dough shape, tabletting, heating with primer until the tablet is golden yellow, cooling, and pulverizing to obtain hepar Caprae Seu Ovis cereal tablet; the product of the invention contains functional polypeptide, soy isoflavone and mutton smell, has unique taste and is easy to be brewed and drunk.)

1. The sheep liver cereal recombinant food is characterized in that 10-15 parts of milk powder, 5-8 parts of sheep liver cereal tablets and 2-4 parts of cane sugar are uniformly mixed under an aseptic condition according to parts by mass and then packaged to prepare the sheep liver cereal recombinant food;

the sheep liver cereal tablet comprises the following raw materials in parts by weight: 10-15 parts of rice flour, 40-50 parts of whole wheat flour, 8-12 parts of whole oat flour, 10-12 parts of whole corn flour, 15-20 parts of millet nutrient solution, 25-30 parts of soybean active substance, and 0.1-1 part of sheep liver homogenate and cell lysate.

2. The lamb liver grain reconstituted food according to claim 1, wherein the rice flour is obtained by crushing rice and sieving the crushed rice with a 60-80 mesh sieve; the whole wheat flour is obtained by crushing wheat and screening the crushed wheat by 40-60 meshes; the whole oat flour is obtained by crushing oats and screening the crushed oats by a sieve of 40-60 meshes; the obtained whole corn flour is obtained by crushing corn and sieving the crushed corn with 60-80 meshes; the milk powder is obtained by filtering organic particles and impurities larger than 150 microns from milk and then performing spray drying.

3. The lamb liver cereal recombinant food as claimed in claim 1, wherein the preparation method of the millet nutrient solution comprises the following steps: the washed millet is soaked and then crushed at low temperature, and water and neutral protease are added for enzymolysis at constant temperature to obtain the millet complete nutrient solution.

4. The lamb liver cereal recombinant food as claimed in claim 3, wherein the water content of millet after soaking is 60-70%, the low-temperature crushing is specifically adding a crushed ice homogenizer at 22000r/min to homogenize into paste, and the constant-temperature enzymolysis temperature is 25-35 ℃.

5. The lamb liver cereal recombinant food as claimed in claim 1, wherein the preparation method of the soybean active substance comprises the following steps: soaking soybean in water for germination, crushing at low temperature, adding water and cellulose, and performing enzymolysis at constant temperature to obtain soybean active substance.

6. The lamb liver cereal recombinant food according to claim 5, wherein the soybean is soaked in water for germination at room temperature of 25-40 ℃, and the soybean is water-cultured at 10-12 ℃ for germination; crushing at low temperature, namely homogenizing at 20000r/min in cold water of 4-8 ℃ to form paste; the constant temperature enzymolysis temperature is 25-35 ℃.

7. The goat liver cereal recombinant food as claimed in claim 1, wherein the preparation method of the goat liver homogenate and the cell lysate comprises the following steps: freezing sheep liver, slicing, thawing with water, and homogenizing at low temperature to obtain sheep liver homogenate and cell lysate.

8. The lamb liver grain recombined food as claimed in claim 7, wherein the refrigeration temperature is-25 ℃ to-35 ℃, and the slice thickness is 3-5 mm; adding 4-8 deg.C water, thawing until ice blocks disappear, and homogenizing at 4-8 deg.C at 220000 r/min.

9. The lamb liver cereal recombinant food as claimed in claim 1, wherein the preparation method of the lamb liver cereal tablet comprises the following steps:

(1) modulation: mixing rice flour, whole wheat flour, whole oat flour, whole millet nutrient solution, soybean active substance, sheep liver homogenate and cell lysate thereof with water, stirring, and mixing to obtain sheep liver cereal product;

(2) and (3) proofing: fermenting the sheep liver cereal product obtained in the step (1) to obtain sheep liver cereal paste;

(3) extrusion forming and baking: and (3) putting the goat liver grain batter obtained in the step (2) into a die with the thickness of 1-10mm, baking at 40-60 ℃ until the batter is in a dough shape, tabletting to the thickness of 2-3mm, heating with a primer until the tablet is golden yellow, taking out, cooling, and crushing to obtain the goat liver grain tablets.

10. The lamb liver cereal recombinant food as claimed in claim 9, wherein the enzymolysis temperature in the step (1) is 25-40 ℃; the proofing temperature in the step (2) is 30-35 ℃, and the proofing time is 10-12 h; in the step (3), the heating temperature of the primer is 100-.

Technical Field

The invention relates to the technical field of food, in particular to a sheep liver cereal recombinant food.

Background

The Renbel is a famous natural pasture in the world and is a green clean soil without any pollution, more than 120 kinds of nutritious pastures such as soda grass, stipa, alfalfa, wheatgrass and the like and Chinese herbal medicines grow in the natural pasture, so that high-quality cattle and sheep resources are bred, the Renbel cattle and sheep products are favored by consumers at home and abroad with unique excellent quality, and the milk and the sheep liver of the Renbel are excellent products in the same class.

The sheep liver extract has the nutrient elements required by human body, and also has the effects of nourishing liver, improving eyesight, replenishing blood, and clearing deficiency heat. The formula is written in the treatise of the drug property treatise of the eyesight of the liver of the green sheep, the formula is written in the treatise of the liver tonifying and the eyesight improving in the treatment of the thousand gold and the food therapy, and the formula is written in the formula of the Tang materia medica to treat the liver wind deficiency heat. Seven patients eat the liver without seeing the eyes. "Shi practical TCM" also says that it is applicable to vitamin A deficiency of eye diseases (infantile malnutrition, night blindness, etc.) in sheep liver. However, the goat liver has great goat odor, and the taste of the food and the popularity of consumers are influenced by the goat liver-containing food due to the strong goat odor. How to remove the mutton smell of the food containing the sheep liver and improve the taste is a technical problem to be solved urgently by technical personnel in the field.

The grains mainly comprise rice, wheat, soybean and other coarse cereals. The grains are processed into staple food, and 50-80% of heat energy, 40-70% of protein and more than 60% of vitamin B1 are provided for human beings. Cereals, as the traditional diet of Chinese people, have been one of the indispensable foods on dining tables of common people for thousands of years, and have an important position in diet of China.

In the prior art, no report of preparing the reconstituted milk beverage by taking sheep liver, grains and milk as main raw materials is found.

Disclosure of Invention

The invention aims to provide a goat liver grain recombined food which is prepared by taking goat liver, grains and milk as main raw materials, contains functional polypeptide, soybean isoflavone and mutton smell, has unique taste and is easy to be brewed and drunk.

The invention provides a sheep liver grain recombinant food, which is prepared by uniformly mixing 10-15 parts of milk powder, 5-8 parts of sheep liver grain tablets and 2-4 parts of cane sugar in parts by mass under an aseptic condition and packaging;

the sheep liver cereal tablet comprises the following raw materials in parts by weight: 10-15 parts of rice flour, 40-50 parts of whole wheat flour, 8-12 parts of whole oat flour, 10-15 parts of whole corn flour, 15-20 parts of millet nutrient solution, 25-30 parts of soybean active substance, and 0.1-1 part of sheep liver homogenate and cell lysate.

Further, the rice flour is obtained by crushing rice and sieving the crushed rice with a 60-80-mesh sieve; the whole wheat flour is obtained by crushing wheat and screening the crushed wheat by 40-60 meshes; the whole oat flour is obtained by crushing oats and screening the crushed oats by a sieve of 40-60 meshes; the obtained whole corn flour is obtained by crushing corn and sieving the crushed corn with 60-80 meshes; the milk powder is obtained by filtering organic particles and impurities larger than 150 microns from milk and then performing spray drying.

The beneficial active substances in the rice, the wheat, the oat and the corn are released after being crushed, so that the subsequent enzymolysis and conversion and the digestion and absorption of nutrient substances are facilitated.

Further, the preparation method of the millet nutrient solution comprises the following steps: the washed millet is soaked and then crushed at low temperature, and water and neutral protease are added for enzymolysis at constant temperature to obtain the millet complete nutrient solution.

Further, the water content of the millet after soaking is 60-70%, the low-temperature crushing is specifically to add a crushed ice homogenizer at 22000r/min to homogenize into paste, and the constant-temperature enzymolysis temperature is 25-35 ℃.

The content of the protein in the millet is as high as 4.88-15.88%, the millet protein is a rich source of methionine, isoleucine, leucine, phenylalanine and other essential amino acids, and the content of the essential amino acids accounts for 44.70% of the total amino acids; after the millet is soaked in water, the millet is reacted with neutral protease to promote the full enzymolysis of protein in the millet, so that the nutrient substances are fully released, the nutritional efficacy of the product is improved, and the millet full nutrient solution containing the polypeptide is obtained.

Further, the preparation method of the soybean active substance comprises the following steps: soaking soybean in water for germination, crushing at low temperature, adding water and cellulase, and performing enzymolysis at constant temperature to obtain soybean active substance.

Further, the water soaking and germinating process of the soybeans comprises the steps of water-culturing the soybeans for 60-72 hours at 10-12 ℃ at the room temperature of 25-40 ℃ to germinate the soybeans; crushing at low temperature, specifically adding crushed ice into cold water at normal temperature, and homogenizing at 20000r/min to obtain paste (avoiding damage of active ingredients of soybean sprout caused by local high temperature of high-speed homogenization); the constant temperature enzymolysis temperature is 25-35 ℃.

During the water absorption and germination of the soybeans, the activity of the protease is increased, so that the storage protein in the soybeans is hydrolyzed, and free amino acid and amide are generated along with the degradation of the storage protein, so that the nutritive value of the soybeans is improved. Then enzymolysis is carried out on cell walls at constant temperature by cellulase, so that flavonoid active substances in the cell walls are fully dissolved out.

Further, the preparation method of the sheep liver homogenate and the cell lysate comprises the following steps: freezing sheep liver, slicing, thawing with water, and homogenizing at low temperature to obtain sheep liver homogenate and cell lysate.

Further, the refrigeration temperature is-25 ℃ to-35 ℃, and the slice thickness is 3-5 mm; adding 4-8 deg.C water, thawing until ice blocks disappear, and homogenizing at 4-8 deg.C at 220000 r/min.

Further, the preparation method of the sheep liver cereal tablet comprises the following steps:

(1) modulation: mixing rice flour, whole wheat flour, whole oat flour, whole millet nutrient solution, soybean active substance, sheep liver homogenate and cell lysate thereof with water, stirring, and mixing to obtain sheep liver cereal product;

(2) and (3) proofing: fermenting the sheep liver cereal product obtained in the step (1) to obtain sheep liver cereal paste;

(3) extrusion forming and baking: and (3) putting the goat liver grain batter obtained in the step (2) into a die with the thickness of 1-10mm, baking at 40-60 ℃ until the batter is in a dough shape, tabletting to the thickness of 2-3mm, heating with a primer until the tablet is golden yellow, taking out, cooling, and crushing to obtain the goat liver grain tablets.

Further, the enzymolysis temperature in the step (1) is 25-40 ℃; the proofing temperature in the step (2) is 30-35 ℃, and the proofing time is 10-12 h; in the step (3), the heating temperature of the primer is 100-.

Compared with the prior art, the invention has the following beneficial effects:

the sheep liver and grain recombined food prepared by the invention is scientifically compatible with sheep liver, grains and milk. The combination of rice flour, whole wheat flour, whole oat flour, whole corn flour, millet nutrient solution, soybean active substances, sheep liver homogenate and cell lysate and baking into formula and process requirements enable various substances to interact, and the product does not have the mutton smell of mutton and is easy to accept by consumers; unique flavor, and more increase the mouthfeel and appetite of consumers. The beverage contains functional polypeptide and soybean isoflavone, and the functional components are derived from food, so that the beverage is safer, and can prevent cardiovascular diseases, osteoporosis of women and the like.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

Example 1

(1) And (3) processing of milk powder: the food grade centrifugal pump delivers milk at 0.5bar pressure to a milk filter which filters out organic particles and other impurities greater than 150 microns.

(2) Processing the rice flour: and crushing the rice by a high-speed crusher at 10000r/min, and sieving by an 80-mesh sieve to obtain the rice flour.

(3) Processing the whole wheat flour: washing wheat to remove impurities, placing qualified wheat in a washing tank, washing with clear water until effluent water becomes clear, draining excessive water, oven drying until water content is 10%, pulverizing with a high-speed pulverizer at 12000r/min, and sieving with a 60-mesh sieve to obtain whole wheat flour for use;

(4) processing the whole oat flour: elutriating oat to remove impurities, placing qualified oat in a cleaning tank, washing with clear water until effluent water becomes clear, draining off excessive water, drying in an oven until the water content is 10%, pulverizing with a high-speed pulverizer 16000r/min, and sieving with a 40-mesh sieve to obtain oat whole wheat flour for later use;

(5) processing whole corn flour: washing the corns to remove impurities, placing the corns in a washing tank, washing with clear water until effluent water becomes clear, draining excessive water, drying in an oven until the water content is 8-15%, crushing by a high-speed crusher at 10000r/min, and sieving by a 40-mesh sieve to obtain whole corn powder for later use;

(6) processing the millet full nutrient solution: washing millet to remove impurities, placing the millet in a washing tank, washing with clear water until effluent water becomes clear, fishing out and soaking for 4 hours, draining excessive water until the water content of the millet reaches 70%, adding a crushed ice homogenizer at 22000r/min to homogenize into paste, adding water and neutral protease at 1.5g/L, and carrying out enzymolysis on protein in the millet at a constant temperature of 35 ℃ to obtain a millet complete nutrient solution containing polypeptide;

(7) processing of soybean active substances: washing semen glycines with clear water, culturing at room temperature of 25 deg.C with 12 deg.C water for 70 hr, breaking at low temperature, adding 8 deg.C cold water 20000r/min, homogenizing to obtain paste, adding water and cellulase (3.8g/L), and performing enzymolysis at 35 deg.C to remove cell wall to release more soybean isoflavone active substances;

(8) processing of sheep liver homogenate and cell lysate: cutting frozen hepar Caprae Seu Ovis at-30 deg.C into slices of 5mm thickness, adding clear water of 8 deg.C; thawing until ice blocks disappear; homogenizing at 8 deg.C and low temperature at 22000r/min to obtain hepar Caprae Seu Ovis homogenate and cell eluate;

(9) modulation: taking 10 parts of rice flour, 40 parts of wheat whole wheat flour, 10 parts of oat whole wheat flour, 10 parts of corn whole flour, 15 parts of millet whole nutrient solution, 25 parts of soybean active substance, 0.5 part of sheep liver homogenate and cell lysate thereof, adding a proper amount of water, and uniformly stirring and mixing to ensure that the product has the quality of the product preliminarily;

(10) and (3) proofing: the prepared and enzymolyzed sheep liver cereal product is transferred into a fermentation box and fermented for 12 hours at 35 ℃;

(11) extrusion molding: injecting the goat liver grain batter into a square baking mould box under the heating condition of 60 ℃, and quickly tabletting the goat liver grain batter to the thickness of 3mm when the goat liver grain tabletting batter is in a dough shape;

(12) baking and cooling: heating with fire at 150 deg.C to obtain golden yellow tablet, rapidly taking out, and cooling under aseptic condition;

(13) and (3) small tabletting: crushing the cooled tablets under aseptic conditions, and drying small tablets with the diameter of 3mm by a self-made engineering sieve for later use;

(14) three nutrient combinations: under the aseptic condition, 10 parts of milk powder, 8 parts of sheep liver cereal tablets and 3 parts of cane sugar are mixed in proportion, the metal detection is qualified, and the mixture is packaged in small bags.

Comparative example 1

The oat whole meal of example 1 was removed and the process of example 1 was repeated;

comparative example 2

The whole nutrient solution of the millet in the example 1 is removed, and the process is the same as the example 1;

comparative example 3

The soybean active material prepared in example 1 was removed, and the procedure of example 1 was otherwise repeated;

comparative example 4

The step (10) in the example 1 is removed, and the process is the same as the example 1;

comparative example 5

The enzymolysis step in the step (6) is removed, and the steps are the same as those in the embodiment 1;

comparative example 6

The enzymolysis step in the step (7) is removed, and the steps are the same as those in the embodiment 1;

comparative example 7

The step of sprouting soybeans in the step (7) is removed, and the steps are the same as those in the example 1;

the advantageous effects of the present invention can be demonstrated by the following tests.

Effect verification 1 polypeptide test

And (3) preparing a standard curve: taking 10-mL volumetric flasks, preparing 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6 and 1.8mg/mL Gly-Gly-Tyr-Arg tetrapeptide standard solutions by using 5% TCA (trichloroacetic acid), respectively taking 4.0mL biuret reagents, uniformly mixing, standing for 10min, centrifuging at 2000r/min for 10min, taking supernatant fluid at 540nm, measuring an OD value, and taking the concentration of the peptide as an abscissa and the OD value as an ordinate to prepare a standard curve.

And (3) determination of polypeptide content: taking 2.5g of cereal pieces, adding 2.5mL of 10% TCA, uniformly mixing on a vortex mixer, carrying out water bath at 30 ℃ for 30min, standing for 10min, centrifuging at 4000r/min for 15min, transferring all supernate into a 50mL volumetric flask, adding 5% TCA to constant volume, and shaking up. And then taking 6.0mL of the solution, adding 4.0mL of biuret reagent into a vortex mixer, uniformly mixing, standing for 10min, centrifuging at 2400r/min for 10min, taking the supernatant fluid at 540nm to measure the OD value, and obtaining the polypeptide content in the sample solution on a standard curve. The results are shown in Table 1.

TABLE 1

The result shows that the sheep liver grain recombinant food prepared by the method contains polypeptide.

Effect verification 2 measurement of flavone

Preparation of a standard solution: 13.2mg of analytically pure rutin is weighed, and the volume of 60 percent ethanol is determined to be 25 ml.

Drawing a standard curve: accurately sucking 0.0 ml, 0.4 ml, 0.8 ml, 1.2 ml, 1.6 ml and 2.0ml of standard solution, transferring into a 10ml graduated colorimetric tube, respectively adding 60% ethanol solution to 2ml, respectively adding 0.5ml of 5% sodium nitrite solution, shaking, standing for 6min, adding 0.5ml of 10% aluminum nitrate solution, shaking uniformly, standing for 6min, adding 4.0ml of 1.0mol/L sodium hydroxide solution, and fixing the volume to the graduation by using 60% ethanol; shaking, standing for 15min, measuring absorbance at 510nm wavelength, and taking 0.0 tube as blank and rutin content as abscissa; a standard curve was plotted with the absorbance as the ordinate.

Taking 50g of product, crushing to obtain sample powder, weighing 0.8g of the sample powder, placing the sample powder in a 100ml round bottom flask, adding 60ml of 60% ethanol solution for infiltration, refluxing for 60min in 70 ℃ water bath, filtering to fix the volume to 100ml, sucking 1.0ml, placing in a 10ml graduated colorimetric tube, adding 60% ethanol solution to 2ml, adding 0.5ml of 5% sodium nitrite solution, placing for 6min after shaking, adding 0.5ml of 10% aluminum nitrate solution, placing for 6min after shaking uniformly, adding 4.0ml of 1.0mol/L sodium hydroxide solution, fixing the volume to the graduation by using 60% ethanol. Shaking, standing for 15min, measuring absorbance at 510nm wavelength, and measuring flavone content in the sample by using standard curve. The results are shown in Table 2.

TABLE 2

Effect verification 3 removal of mutton smell and taste test

The goat liver cereal recombinant food prepared in the example 1 and the comparative examples 1-8 is mixed with warm water, and a sensory evaluator trained professionally is selected to evaluate the flavor and taste of the goat liver cereal recombinant food. The results of the evaluation are summarized in Table 3.

TABLE 3

Example 2

The same as example 1, except that in step (9), 15 parts of rice flour, 50 parts of whole wheat flour, 8 parts of whole oat flour, 12 parts of whole corn flour, 20 parts of whole millet nutrient solution and 30 parts of soybean active substance, and 1 part of sheep liver homogenate and cell lysate thereof are used.

And (5) mixing 15 parts of milk powder, 5 parts of sheep liver cereal tablets and 4 parts of cane sugar in proportion in the step (14).

Example 3

The same as example 1, except that 12 parts of rice flour, 45 parts of whole wheat flour, 12 parts of whole oat flour, 12 parts of whole corn flour, 18 parts of whole millet nutrient solution and 18 parts of soybean active substance in step (9), and 0.1 part of sheep liver homogenate and cell lysate thereof.

And (5) blending 12 parts of milk powder, 7 parts of sheep liver cereal tablets and 4 parts of cane sugar in proportion in the step (14).

Example 4

The same as example 1, except that in step (9), 12 parts of rice flour, 42 parts of whole wheat flour, 18 parts of whole oat flour, 11 parts of whole corn flour, 17 parts of whole millet nutrient solution, 30 parts of soybean active substance, and 0.8 part of sheep liver homogenate and cell lysate thereof.

And (5) blending 11 parts of milk powder, 7 parts of sheep liver cereal tablets and 2 parts of cane sugar in proportion in the step (14).

The products of examples 2 to 4 were subjected to the verification of effect verification 1 to 2, and the results showed that the same technical effects as those of example 1 were obtained.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included therein.