阅读说明：本技术 一种谷物霉菌毒素脱除方法 (Method for removing mycotoxin from grains ) 是由 程勐万里 孟龙 符秀迪 郭彦林 程淦 于 2020-12-25 设计创作，主要内容包括：本发明提供一种谷物霉菌毒素脱除方法,属于生物技术与饲料加工领域,本发明将谷物与干净水按比例混合,搅拌浸提、静置分层后使用霉菌毒素吸附袋进行毒素吸附脱除。通过小孔径尼龙袋能够阻拦其他生物大分子进入,而小分子霉菌毒素能够顺利进入被载体吸附的特点提供一种脱除效果好、提升谷物质量、提高营养价值、改变副产物利用途径的霉菌毒素脱除方法。本发明旨在解决传统霉菌毒素吸附法存在的影响饲料适口性、增大饲粮灰分、降低牲畜采食量、降低饲料营养价值、吸附效果差等诸多问题,提供一种脱除效果好、提升谷物质量、提高营养价值、改变副产物利用途径的霉菌毒素脱除方法。(The invention provides a method for removing mycotoxin from cereal, which belongs to the field of biotechnology and feed processing. The small-aperture nylon bag can prevent other biomacromolecules from entering, and the small-molecule mycotoxin can smoothly enter and be adsorbed by the carrier, so that the method for removing the mycotoxin has the characteristics of good removing effect, grain quality improvement, nutritive value improvement and change of a byproduct utilization path. The invention aims to solve the problems of influence on feed palatability, increase of feed ash content, reduction of feed intake of livestock, reduction of feed nutritive value, poor adsorption effect and the like of the traditional mycotoxin adsorption method, and provides the mycotoxin removal method which has a good removal effect, improves grain quality, improves nutritive value and changes a byproduct utilization way.)

1. A method for removing mycotoxin from cereals is characterized by comprising the following steps:

1) mixing the grains and clean water in proportion to obtain mixed feed liquid;

2) after the mixed material liquid is stirred and extracted for a certain time, stopping stirring, standing and layering, wherein the upper layer is a turbid water layer, and the lower layer is a grain layer;

3) preparing a plurality of nylon cloth bags in advance, mixing a plurality of adsorbents in the cloth bags in proportion, and sealing the cloth bags to obtain mycotoxin adsorption bags;

4) putting a certain number of mycotoxin adsorption bags into the water turbid liquid layer according to the weight ratio of the ton raw material to the number of the nylon bags, stirring the water turbid liquid layer or stirring the mycotoxin adsorption bags, and continuously stirring for a certain time to ensure the adsorption effect;

5) and taking out the mycotoxin adsorption bag, separating the grains from the turbid water, drying the grains after toxin removal, and storing the grains, or directly using the grains after toxin removal to enter a downstream production process.

2. A method for removing mycotoxin from cereals is characterized by comprising the following steps:

1) mixing the grains and clean water in proportion to obtain mixed feed liquid;

2) after the mixed material liquid is stirred and extracted for a certain time, stopping stirring, standing and layering, wherein the upper layer is water turbid liquid, the lower layer is grain, and the grain and the water turbid liquid are separated again;

3) preparing a plurality of nylon cloth bags in advance, mixing a plurality of adsorbents in the cloth bags in proportion, and sealing the cloth bags to obtain mycotoxin adsorption bags;

4) putting a certain amount of mycotoxin adsorption bags into the separated turbid water solution according to the ratio of the weight of the ton raw materials to the number of the nylon bags, stirring the turbid water solution or the mycotoxin adsorption bags, and continuously stirring for a certain time to ensure the adsorption effect;

5) and taking out the mycotoxin adsorption bag, separating the grains from the turbid water, drying the grains after toxin removal, and storing the grains, or directly using the grains after toxin removal to enter a downstream production process.

3. The method for removing mycotoxin from cereal according to claim 1 or 2, wherein the cereal in step 1) is a grain seed comprising corn, wheat and rice, and the weight ratio of the cereal to water is 1: 1-20.

4. The method for removing mycotoxin from cereal according to claim 1 or 2, wherein the cereal in step 1) is pulverized cereal flour, and the weight ratio of the cereal flour to water is 1: 2-5.

5. The method for removing mycotoxin from cereal as claimed in claim 4, wherein the cereal in step 1) is a pulverized cereal flour having a particle size of: the 30-mesh standard sieve passage rate is 0-80%, and the 10-mesh standard sieve passage rate is 0-100%.

6. The method for removing mycotoxin from cereal as claimed in claim 5, wherein the cereal in step 1) is a pulverized cereal flour having a particle size of: the 30-mesh standard sieve passage rate is 30-50%, and the 10-mesh standard sieve passage rate is 100%.

7. The method for removing mycotoxin in cereal according to claim 1 or 2, wherein the adsorbent in step 3) comprises one or more of modified montmorillonite, yeast cell wall, activated carbon and medical stone.

8. The method of claim 7, wherein the adsorbent has a particle size of: the passing rate of a 300-mesh standard sieve is 1-100%, and the passing rate of a 30-mesh standard sieve is 100%;

the weight of the ton of raw materials and the number of the nylon bags in the step 4) are as follows: 1: 0.1-2; the continuous stirring time was: 1-120 minutes;

the stirring and leaching time in the step 2) is 1-120 minutes;

the size of the nylon cloth bag in the step 3) is as follows: the length is 5-50 cm, and the width is 5-50 cm; the aperture of the cloth bag is as follows: 100 to 5000 meshes.

9. The method for removing mycotoxin in cereal as claimed in claim 8, wherein the adsorbent is modified montmorillonite, yeast cell walls, activated carbon and medical stone, and the weight ratio of the modified montmorillonite to the yeast cell walls to the activated carbon to the medical stone is 1: 0-1: 0-1: 0 to 1; the particle size of the adsorbent is as follows: the passing rate of the 300-mesh standard sieve is 50-70%, and the passing rate of the 30-mesh standard sieve is 100%;

the weight of the ton of raw materials and the number of the nylon bags in the step 4) are as follows: 1: 0.5-1; the continuous stirring time was: 10-60 minutes;

the stirring and leaching time in the step 2) is 3-30 minutes;

the size of the nylon cloth bag in the step 3) is as follows: the length is 10-20 cm, and the width is 10-20 cm; the aperture of the cloth bag is as follows: 2000 to 4000 meshes.

10. The method for removing mycotoxin in cereal as defined in claim 9, wherein the weight ratio of the modified montmorillonite, the yeast cell wall, the activated carbon and the medical stone is 1: 0.1-0.2: 0.2-0.5: 0.2 to 0.5.

Technical Field

The invention belongs to the technical field of feeds, and particularly relates to a method for removing mycotoxin from grains.

Background

China is a livestock breeding country and a population country. The quality problem of grain directly affects the feed quality safety problem of the livestock breeding industry and the food quality safety problem of 14 hundred million people in China. The mycotoxin problem is particularly prominent in the quality problems of grain grains, and according to the analysis of mycotoxin pollution conditions of feeds and raw grains in 2019, the mycotoxin limit values in the feed health standard (GB 13078-: in 2019, mycotoxin pollution conditions of different degrees exist in feed and feed raw materials (corn and byproducts thereof, wheat, cake meal and the like), particularly most concerned vomitoxin, zearalenone toxin and aflatoxin exceed a limit standard by tens of times. The total output of commercial feed in China in 2019 reaches 2.62 million tons, wherein the consumption of corn is 1.78 million tons, the consumption of corn is 6800 million tons in the deep processing industry, the consumption of wheat is 1400 million tons, and the consumption of paddy is 1876 million tons. Solves the problem of mycotoxin pollution of grains, and can obviously improve the food safety of grains and livestock meat from the source.

Vomitoxin widely exists in cereal crops such as wheat, barley, corn and the like, also pollutes grain products, belongs to virulent or moderate toxicants, and can generate wide toxic effects after people and animals eat grains polluted by the vomitoxin by mistake. After people and livestock ingest food/feed contaminated by vomitoxin, acute poisoning symptoms such as anorexia, emesis, diarrhea, fever, unstable standing, slow response and the like can be caused, and the hematopoietic system is damaged to cause death in severe cases. Research shows that vomitoxin may affect the immune system, has obvious embryotoxicity and certain teratogenicity, and may be used together with other mycotoxin, such as aflatoxin, to pollute crop and affect each other after entering human body. In recent years, vomitoxin is discovered to be possibly related to human esophageal cancer and kidney disease, and poses a threat to the health of human beings and animals.

Aflatoxins are a class of compounds with similar chemical structures, and are derivatives of dihydrofurocoumarin. They exist in soil, animals and plants and various nuts, are particularly easy to pollute grain and oil products such as peanuts, corns, rice, soybeans, wheat and the like, and are mycotoxins which have the highest mycotoxin poisoning property and extremely outstanding harm to human health. Aflatoxin was classified as a class 1 carcinogen by the cancer research organization of the World Health Organization (WHO) in 1993 and is a highly toxic substance, the hazard of aflatoxin is that aflatoxin has a destructive effect on human and animal liver tissues, and liver cancer and even death can be caused in severe cases. When the intake of a human is large, acute poisoning may occur, with acute hepatitis, hemorrhagic necrosis, hepatocellular steatosis, and bile duct hyperplasia. When a trace amount of the medicine is continuously taken, chronic poisoning and growth disorder can be caused, fibrous lesion is caused, and fibrous tissue hyperplasia is caused. AFT is also the leading cause of carcinogenesis and is one of the strongest carcinogens currently known. In agricultural production, it is not uncommon for corn to have aflatoxins that exceed the limits. If totally destroyed, it will be a significant loss. At present, the total content of the feed additive can be reduced to a lower range only by mixing the feed additive with a non-standard mixture, but the method can not be used for human food and can only be used for feed. In addition, the aflatoxin can not enter the wine after distillation. The residual vinasse contains a lot of toxins and cannot be used as feed, so that serious resource waste is caused.

Zearalenone toxin mainly pollutes corn, wheat, rice, barley, millet, oat and other grains. Wherein the positive detection rate of the corn is 45 percent, and the highest toxic content can reach 2909 mg/kg; the detection rate of wheat is 20 percent, and the highest toxic content is 11.05 mg/kg. Zearalenone has estrogen-like effect, can cause acute and chronic poisoning of animals, causes abnormal and even death of animal reproductive function, and can cause huge economic loss to animal farms. Mainly acts on reproductive system, and can cause hypermenorrhea of livestock, poultry and experimental mice. Consumption of zearalenone containing foods by pregnant animals (including humans) can cause abortion, stillbirth and teratogenesis. The food made from wheat flour containing gibberellic disease can also cause poisoning symptoms of central nervous system, such as nausea, chill, headache, mental depression and ataxia.

Currently, there are three main methods for treating mildewed grains: physical screening disruption methods, chemical degradation methods and biological degradation methods. Physical subtractive grain loss rates are too high at about 10% -30%; chemical degradation methods can destroy many other organic nutrients at the same time; the biodegradation method has high energy consumption and poor effect; the most mature and widely applied mode is an adsorption method, and an adsorbent is directly added during feed production or use to adsorb mycotoxin in the feed and is tightly combined with the mycotoxin, so that the mycotoxin is not absorbed and directly discharged out of a body when being in an animal digestive tract, and the aim of avoiding the harm of the mycotoxin to animals is fulfilled. Currently, the common mycotoxin adsorbent is mainly natural aluminosilicate (such as montmorillonite), or montmorillonite and organic adsorbent (such as glucomannan) are modified and compounded. However, this method has significant disadvantages: firstly, the direct addition of adsorbent can influence the fodder palatability, increases the fodder ash content, reduces livestock feed intake. And secondly, the adsorbent can simultaneously adsorb vitamin, mineral substance and other micromolecular nutrients, so that the nutritional value of the feed is reduced. Thirdly, the adsorbent is mixed in dry feed, can not contact with mycotoxin to play a role, and can only contact with the mycotoxin to play an adsorption role in an animal body, but biochemical reaction in the digestive tract of the animal is complex, and the adsorption effect and stability of the mycotoxin can not be ensured, so that the using effect is poor.

When the feed with high mycotoxin content is used for feeding animals for a long time, the resistance of the animals is obviously reduced, and diseases such as diarrhea and the like are very easy to occur. For years, feed enterprises are used to add antibiotics into feed to prevent diseases such as pig diarrhea. The medicinal feed additive will exit the history stage in 2020. The disablement of feed antibiotics is not an easy task, and as the concept of food safety deepens, mycotoxin removal technology will bring benefits to the field in the future.

Disclosure of Invention

The invention provides a method for removing mycotoxin from grains, and aims to solve the problems of influence on feed palatability, increase of feed ash content, reduction of feed intake of livestock, reduction of feed nutritive value, poor adsorption effect and the like of a traditional mycotoxin adsorption method. Other biomacromolecules are prevented from entering through the nylon bag with the small aperture, and the small-molecule mycotoxin can smoothly enter and be adsorbed by the carrier, so that the method for removing the mycotoxin is good in removing effect, improves the quality of grains, improves the nutritional value and changes the utilization path of byproducts.

The technical scheme adopted by the invention is as follows:

a method for removing a cereal mycotoxin comprises the following steps:

1. a method for removing mycotoxin from cereals is characterized by comprising the following steps:

1) mixing the grains and clean water in proportion to obtain mixed feed liquid;

2) after the mixed material liquid is stirred and extracted for a certain time, stopping stirring, standing and layering, wherein the upper layer is a turbid water layer, and the lower layer is a grain layer;

3) preparing a plurality of nylon cloth bags in advance, mixing a plurality of adsorbents in the cloth bags in proportion, and sealing the cloth bags to obtain mycotoxin adsorption bags;

4) putting a certain number of mycotoxin adsorption bags into the water turbid liquid layer according to the weight ratio of the ton raw material to the number of the nylon bags, stirring the water turbid liquid layer or stirring the mycotoxin adsorption bags, and continuously stirring for a certain time to ensure the adsorption effect;

5) and taking out the mycotoxin adsorption bag, separating the grains from the turbid water, drying the grains after toxin removal, and storing the grains, or directly using the grains after toxin removal to enter a downstream production process.

Or a method for removing the mycotoxin from the cereal, which comprises the following steps:

1) mixing the grains and clean water in proportion to obtain mixed feed liquid;

2) after the mixed material liquid is stirred and extracted for a certain time, stopping stirring, standing and layering, wherein the upper layer is water turbid liquid, the lower layer is grain, and the grain and the water turbid liquid are separated again;

3) preparing a plurality of nylon cloth bags in advance, mixing a plurality of adsorbents in the cloth bags in proportion, and sealing the cloth bags to obtain mycotoxin adsorption bags;

4) putting a certain amount of mycotoxin adsorption bags into the separated turbid water solution according to the ratio of the weight of the ton raw materials to the number of the nylon bags, stirring the turbid water solution or the mycotoxin adsorption bags, and continuously stirring for a certain time to ensure the adsorption effect;

5) and taking out the mycotoxin adsorption bag, separating the grains from the turbid water, drying the grains after toxin removal, and storing the grains, or directly using the grains after toxin removal to enter a downstream production process.

Furthermore, the grains in the step 1) are particle grains and comprise corn, wheat and rice, and the weight ratio of the grains to water is 1: 1-20.

Furthermore, the cereal in the step 1) is pulverized cereal powder, and the weight ratio of the cereal powder to water is 1: 2-5.

Further, the grain in step 1) is a pulverized grain flour, and the grain size of the grain flour is as follows: the 30-mesh standard sieve passage rate is 0-80%, and the 10-mesh standard sieve passage rate is 0-100%.

Further, the grain in step 1) is a pulverized grain flour, and the grain size of the grain flour is as follows: the 30-mesh standard sieve passage rate is 30-50%, and the 10-mesh standard sieve passage rate is 100%.

7. The method for removing mycotoxin in cereal according to claim 1 or 2, wherein the adsorbent in step 3) comprises one or more of modified montmorillonite, yeast cell wall, activated carbon and medical stone.

Further, the adsorbent particle size is: the passing rate of a 300-mesh standard sieve is 1-100%, and the passing rate of a 30-mesh standard sieve is 100%;

the weight of the ton of raw materials and the number of the nylon bags in the step 4) are as follows: 1: 0.1-2; the continuous stirring time was: 1-120 minutes;

the stirring and leaching time in the step 2) is 1-120 minutes;

the size of the nylon cloth bag in the step 3) is as follows: the length is 5-50 cm, and the width is 5-50 cm; the aperture of the cloth bag is as follows: 100 to 5000 meshes.

Preferably, the adsorbent is modified montmorillonite, yeast cell walls, activated carbon and medical stones, and the weight ratio of the modified montmorillonite to the yeast cell walls to the activated carbon to the medical stones is 1: 0-1: 0-1: 0 to 1; the particle size of the adsorbent is as follows: the passing rate of the 300-mesh standard sieve is 50-70%, and the passing rate of the 30-mesh standard sieve is 100%;

the weight of the ton of raw materials and the number of the nylon bags in the step 4) are as follows: 1: 0.5-1; the continuous stirring time was: 10-60 minutes;

the stirring and leaching time in the step 2) is 3-30 minutes;

the size of the nylon cloth bag in the step 3) is as follows: the length is 10-20 cm, and the width is 10-20 cm; the aperture of the cloth bag is as follows: 2000 to 4000 meshes.

Furthermore, the weight ratio of the modified montmorillonite to the yeast cell wall to the active carbon to the medical stone is 1: 0.1-0.2: 0.2-0.5: 0.2 to 0.5.

Further, the mixed material liquid in the step 1) is adjusted to be alkaline, such as calcium hydroxide is added.

Compared with the prior art, the invention has the following beneficial effects:

(1) the method comprises the steps of stirring and leaching the grains with water, dissolving and leaching most of toxins and micro particles with high toxin content into turbid water, adsorbing mycotoxin in the turbid water by using a nylon adsorption cloth bag, and putting the washed and leached grains into the later stage of working procedure.

(2) The adsorbent is separated from the grain by the nylon cloth bag, so that the adsorption effect of the mycotoxin is ensured, the ash content of the grain is not increased, the mycotoxin is removed before the grain is put into feed production and feeding, the adsorbent is not doped, and the palatability and the nutritional value of the grain are not influenced.

(3) Compared with other detoxification methods, the detoxification method has more remarkable detoxification effect, has good feasibility in grain deep processing enterprises, remarkably improves the quality of grain byproducts, reduces the content of mycotoxin, enables the grain byproducts to be used for livestock breeding, and avoids grain waste.

(4) The method is easy to operate, low in consumption, suitable for large-scale production and the most ideal method for removing the mycotoxin.

Detailed Description

The present example takes wheat as an example:

example 1: a method for removing a cereal mycotoxin comprises the following steps:

1) selecting 2kg of wheat with higher vomitoxin content, randomly dividing the wheat into 2 parts, wherein each part is 1kg, one part is used for testing, and the other part is used for blank control.

2) And mixing 1kg of wheat with 2kg of clean water to obtain mixed feed liquid.

3) And after stirring and leaching the mixed material liquid for 30 minutes, stopping stirring, standing and layering for 5 minutes, wherein the upper layer is water turbid liquid, and the lower layer is wheat particles.

4) Preparing 2 nylon cloth bags in advance, mixing 10g of modified montmorillonite, 5g of activated carbon and 2g of yeast cell wall in the cloth bags, and sealing the cloth bags to obtain the mycotoxin adsorption bags, wherein the size of each cloth bag is 10cm x 10cm, the aperture of each cloth bag is 3000 meshes.

5) And putting 2 nylon cloth bags into the turbid water solution, stirring the turbid water solution to immerse the adsorption bags in the turbid water solution for rotation, and continuously stirring for 30 minutes to ensure the adsorption effect.

6) And the mixed material liquid after toxin removal enters the post-working procedure.

Example 2: a method for removing a cereal mycotoxin comprises the following steps:

1) selecting 2kg of wheat with high zearalenone toxin content, randomly dividing into 2 parts, wherein each part is 1kg, one part is used for testing, and the other part is used for blank control.

2) And mixing 1kg of wheat with 3kg of clean water to obtain mixed feed liquid.

3) And after stirring and leaching the mixed material liquid for 10 minutes, stopping stirring, standing and layering for 5 minutes, wherein the upper layer is water turbid liquid, and the lower layer is wheat particles, and separating the wheat particles from the water turbid liquid.

4) Preparing 2 nylon cloth bags in advance, wherein the size of each cloth bag is 10cm x 20cm, the aperture of each cloth bag is 3000 meshes, mixing 9g of modified montmorillonite, 2g of activated carbon, 5g of yeast cell wall and 1g of medical stone in each cloth bag, and sealing the cloth bags to obtain the mycotoxin adsorption bags.

5) And putting 2 nylon cloth bags into the turbid water solution, stirring the turbid water solution to immerse the adsorption bags in the turbid water solution for rotation, and continuously stirring for 20 minutes to ensure the adsorption effect.

6) And the mixed material liquid after toxin removal enters the post-working procedure.

Example 3: a method for removing a cereal mycotoxin comprises the following steps:

1) selecting 2kg of wheat with high aflatoxin content, randomly dividing into 2 parts, wherein each part is 1kg, one part is used for test, and the other part is used for blank control.

2) And mixing 1kg of wheat with 5kg of clean water to obtain mixed feed liquid.

3) And after stirring and leaching the mixed material liquid for 10 minutes, stopping stirring, standing and layering for 5 minutes, wherein the upper layer is water turbid liquid, and the lower layer is wheat particles, and separating the wheat particles from the water turbid liquid.

4) Preparing 2 nylon cloth bags in advance, wherein the size of each cloth bag is 10cm x 10cm, the aperture of each cloth bag is 4000 meshes, mixing 9g of modified montmorillonite, 2g of activated carbon, 5g of yeast cell wall and 1g of medical stone in each cloth bag, and sealing the cloth bags to obtain the mycotoxin adsorption bags.

5) And putting 2 nylon cloth bags into the turbid water solution, stirring the turbid water solution to immerse the adsorption bags in the turbid water solution for rotation, and continuously stirring for 30 minutes to ensure the adsorption effect.

6) And the mixed material liquid after toxin removal enters the post-working procedure.

Comparative example: the blank control wheat of examples 1 to 3 was subjected to the same procedure with the same elements and procedures as those of the examples except that the nylon cloth bag contained no adsorbent.

Blank control and experimental group in operation examples I, II and III are repeated 6 times, 36 groups of wheat mixed feed liquid are collected, dried and crushed, and the content of various mycotoxins in the sample is determined by using methods of determination of aflatoxin B group and G group in food safety national standard food (GB5009.22-2016), determination of deoxynivalenol in food safety national standard food (GB5009.111-2016) and determination of zearalenone in food safety national standard food (GB 5009.209-2016). As can be seen from table 1, compared with the comparative group, the mycotoxin removal method provided by the present invention can significantly reduce the content of mycotoxin in grains and improve the grain quality.

Table one: comparison of mycotoxin detection results of experimental group wheat and control group

As can be seen from the above table, compared with the control group, in the embodiments 1 to 3, the method for removing mycotoxin provided by the present invention can greatly reduce the content of mycotoxin in the raw material, the removal of mycotoxin is obvious, and a simple process is adopted to achieve a very high mycotoxin removal rate, which is just the efficiency and the suggested process pursued at present.

The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope defined by the claims.