阅读说明：本技术 一种戊二醛癸甲溴铵溶液及其制备方法和应用 (Glutaraldehyde decamethylammonium bromide solution and preparation method and application thereof ) 是由 周应培 刘继根 王洋 于 2020-12-10 设计创作，主要内容包括：本发明公开了一种戊二醛癸甲溴铵溶液及其制备方法和应用,属于消毒剂技术领域,该戊二醛癸甲溴铵溶液包括由以下原料组分及重量百分数制成：戊二醛4-7％、癸甲溴铵4-7％、高碳脂肪醇聚氧乙烯醚0.2-2%、丙三醇6-20%和余量去离子水。其制备方法包括以下步骤：S1、称取；S2、将戊二醛和癸甲溴铵混合,得混合物A；S3、将脂肪醇聚氧乙烯醚和丙三醇加入去离子水,然后加入混合物A,搅拌混匀后得到混合物B；S4、调节步骤S2所得混合物B的pH值,加入去离子水定容,得到戊二醛癸甲溴铵溶液。戊二醛癸甲溴铵溶液可应用于消毒剂领域。本发明无刺激性气味,稳定性高,可长期储存,在低温条件下也可保持显著杀菌消毒效果。(The invention discloses a glutaraldehyde decamethylammonium bromide solution as well as a preparation method and application thereof, belonging to the technical field of disinfectants, wherein the glutaraldehyde decamethylammonium bromide solution comprises the following raw material components in percentage by weight: 4-7% of glutaraldehyde, 4-7% of decamethylammonium bromide, 0.2-2% of high-carbon fatty alcohol polyoxyethylene ether, 6-20% of glycerol and the balance of deionized water. The preparation method comprises the following steps: s1, weighing; s2, mixing glutaraldehyde and ammonium decamethylammonium bromide to obtain a mixture A; s3, adding fatty alcohol-polyoxyethylene ether and glycerol into deionized water, then adding the mixture A, and stirring and uniformly mixing to obtain a mixture B; s4, adjusting the pH value of the mixture B obtained in the step S2, and adding deionized water to a constant volume to obtain a glutaraldehyde decamethyl ammonium bromide solution. The glutaraldehyde decamethylammonium bromide solution can be applied to the field of disinfectants. The invention has no pungent smell, high stability, long storage period and obvious sterilizing effect at low temperature.)

1. A glutaraldehyde decamethylammonium bromide solution is characterized in that: the material is prepared from the following raw materials in percentage by weight:

4-7% of glutaraldehyde, 4-7% of decamethylammonium bromide, 0.2-2% of high-carbon fatty alcohol polyoxyethylene ether, 6-20% of glycerol and the balance of deionized water.

2. The glutaraldehyde decamethylammonium bromide solution of claim 1, wherein: the material is prepared from the following raw materials in percentage by weight:

6% of glutaraldehyde, 6% of decamethylammonium bromide, 0.3% of high-carbon fatty alcohol polyoxyethylene ether, 15% of glycerol and the balance of deionized water.

3. A preparation method of a glutaraldehyde decamethylammonium bromide solution is characterized by comprising the following steps: the method comprises the following steps:

s1, weighing: weighing the raw material components and the weight percentage of the glutaraldehyde decamethylammonium bromide solution according to any one of claims 1-2;

s2, preparation of mixture A: mixing glutaraldehyde and decamethylammonium bromide, and stirring uniformly to obtain a mixture A;

s3, preparation of mixture B: adding fatty alcohol-polyoxyethylene ether and glycerol into 60ml of deionized water, then adding the mixture A, and stirring and uniformly mixing to obtain a mixture B;

s4, preparation of a glutaraldehyde decamethylammonium bromide solution: and (4) adjusting the pH value of the mixture B obtained in the step (S2) to 5.4-6.6, and adding deionized water to a constant volume of 100ml to obtain a glutaraldehyde decamethylammonium bromide solution.

4. The method of claim 4, wherein the solution of glutaraldehyde decamethylammonium bromide is prepared by: in step S4, the pH of the resulting mixture B is adjusted to 6.

5. The application of the glutaraldehyde decamethyl ammonium bromide solution is characterized in that: use of a solution of glutaraldehyde decamethylammonium bromide according to any of claims 1-2 in the field of disinfectants.

6. The use of a solution of glutaraldehyde decamethylammonium bromide according to claim 5, wherein: the glutaraldehyde decamethylammonium bromide solution is used for killing escherichia coli, staphylococcus aureus, candida albicans and bacillus subtilis by 100% at the temperature of-35-0 ℃.

7. The use of a solution of glutaraldehyde decamethylammonium bromide according to claim 6, wherein: the maximum dilution multiple of the glutaraldehyde decamethylammonium bromide solution for 100% killing of escherichia coli, staphylococcus aureus and candida albicans is 1:2000, and the maximum dilution multiple of the glutaraldehyde decamethylammonium bromide solution for 100% killing of bacillus subtilis is 1: 1000.

8. The use of a solution of glutaraldehyde decamethylammonium bromide according to claim 5, wherein: the glutaraldehyde decamethylammonium bromide solution is used for 100 percent killing of pathogenic blue ear disease viruses and pseudorabies viruses.

9. The use of a solution of glutaraldehyde decamethylammonium bromide according to claim 8, wherein: the maximum dilution multiple of the glutaraldehyde decamethyl ammonium bromide solution for 100% killing of the pathogenic porcine reproductive and respiratory syndrome virus is 1: 1000; the maximum dilution multiple of the glutaraldehyde decamethyl ammonium bromide solution for 100% killing of the pseudorabies virus is 1: 500.

10. The use of a solution of glutaraldehyde decamethylammonium bromide according to claim 5, wherein: the glutaraldehyde decamethyl ammonium bromide solution is used for dilution disinfection of different concentrations according to the use scene, and is used for wagon balance and piggery peripheral dilution multiple to be 1:2000, dilution factor of 1:1000 for use in pig farms and vehicles.

Technical Field

The invention relates to the technical field of disinfectants, in particular to a glutaraldehyde decamethylammonium bromide solution and a preparation method and application thereof.

Background

Glutaraldehyde is a high-efficiency disinfectant, and free aldehyde groups of glutaraldehyde bind to amino groups of proteins or enzymes on or in the cell surface to cause a series of reactions, resulting in the death of microorganisms. The reaction rate of the glutaraldehyde, the protein and the enzyme depends on the pH value of the solution, the reaction rate is increased along with the increase of the pH value in the range of 4-9, the sterilization effect of the alkaline glutaraldehyde is obviously stronger than that of the acidic glutaraldehyde, and particularly, the difference is larger when the temperature is lower. However, alkaline glutaraldehyde is unstable, easily polymerizes into polymers, and easily loses activity.

The decamethyl ammonium bromide is double-chain quaternary ammonium salt, belongs to an outer membrane active compound, can dissociate out quaternary ammonium salt cations in a solution state, is combined with phosphate groups with negative charges in bacterial cytoplasmic membrane phospholipid, has a bacteriostatic action at low concentration and has a bactericidal action at high concentration. The bromine ions greatly increase the hydrophilicity and lipophilicity of molecules, can rapidly permeate into a plasma membrane lipid layer and a protein layer, change the permeability of the membrane and achieve the bactericidal effect.

The glutaraldehyde decamethyl ammonium bromide solution is a novel compound disinfectant newly introduced in recent years, integrates the advantages of aldehyde disinfectant and quaternary ammonium salt, has a double-sterilization effect due to the mutual synergy of the aldehyde disinfectant and the quaternary ammonium salt, is a broad-spectrum, high-efficiency and low-toxicity aldehyde disinfectant, has no corrosivity on various materials such as metal, glass, rubber, plastic and the like, has small irritation on skin and mucosa, and has strong penetrability and stable performance. The common glutaraldehyde decamethylammonium bromide exists in the form of hydrate (mostly cyclic structure hydrate), so that glutaraldehyde is volatilized in a short time due to the evaporation of water, and the effective killing time is greatly shortened. The Chinese patent application CN201810637946.7 discloses a glutaraldehyde decamethylammonium bromide solution and a preparation method thereof, the disinfectant mainly comprises glutaraldehyde and decamethylammonium bromide, has the characteristics of no pungent smell, high stability and long-term storage, but does not consider the use under low temperature conditions for treatment. Chinese patent application CN201911421388.1 discloses a glutaraldehyde decamethylammonium bromide compound disinfectant suitable for low-temperature use, which consists of glutaraldehyde, decamethylammonium bromide, a deicing agent and a solvent, wherein the deicing agent adopts ethanol, ethylene glycol and sodium chloride. However, ethanol and ethylene glycol are volatile and ethylene glycol is toxic. And the problem of metal corrosion cannot be thoroughly solved when ethanol, glycol or sodium chloride is used.

In northern areas, the time is long in winter, the climate is cold, the disinfection effect of the disinfectant can be influenced in a low-temperature environment, and the freezing of the disinfection solution can greatly reduce the disinfection result of the disinfectant and even make the disinfectant ineffective. Therefore, there is an urgent need for a glutaraldehyde decamethylammonium bromide solution that has reduced pungent odor, high stability, and can maintain a significant sterilizing effect even in a low-temperature environment.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a glutaraldehyde decamethylammonium bromide solution and a preparation method and application thereof.

In order to achieve the purpose, the invention provides the following technical scheme:

a glutaraldehyde decamethylammonium bromide solution is prepared from the following raw materials in percentage by weight:

4-7% of glutaraldehyde, 4-7% of decamethylammonium bromide, 0.2-2% of high-carbon fatty alcohol polyoxyethylene ether, 6-20% of glycerol and the balance of deionized water.

More preferably: the material is prepared from the following raw materials in percentage by weight:

6% of glutaraldehyde, 6% of decamethylammonium bromide, 0.3% of high-carbon fatty alcohol polyoxyethylene ether, 15% of glycerol and the balance of deionized water.

A preparation method of a glutaraldehyde decamethylammonium bromide solution comprises the following steps:

s1, weighing: weighing the raw material components according to the weight percentage of the raw material components of the glutaraldehyde decamethylammonium bromide solution;

s2, preparation of mixture A: mixing glutaraldehyde and decamethylammonium bromide, and stirring uniformly to obtain a mixture A;

s3, preparation of mixture B: adding fatty alcohol-polyoxyethylene ether and glycerol into 60ml of deionized water, then adding the mixture A, and stirring and uniformly mixing to obtain a mixture B;

s4, preparation of a glutaraldehyde decamethylammonium bromide solution: and (4) adjusting the pH value of the mixture B obtained in the step (S2) to 5.4-6.6, and adding deionized water to a constant volume of 100ml to obtain a glutaraldehyde decamethylammonium bromide solution.

More preferably: in step S4, the pH of the resulting mixture B is adjusted to 6.

The application of the glutaraldehyde decamethyl ammonium bromide solution is to apply the glutaraldehyde decamethyl ammonium bromide solution to the field of disinfectants.

More preferably: the glutaraldehyde decamethylammonium bromide solution is used for killing escherichia coli, staphylococcus aureus, candida albicans and bacillus subtilis by 100% at the temperature of-35-0 ℃.

More preferably: the maximum dilution multiple of the glutaraldehyde decamethylammonium bromide solution for 100% killing of escherichia coli, staphylococcus aureus and candida albicans is 1:2000, and the maximum dilution multiple of the glutaraldehyde decamethylammonium bromide solution for 100% killing of bacillus subtilis is 1: 1000.

More preferably: the glutaraldehyde decamethylammonium bromide solution is used for 100 percent killing of pathogenic blue ear disease viruses and pseudorabies viruses.

More preferably: the maximum dilution multiple of the glutaraldehyde decamethyl ammonium bromide solution for 100% killing of the pathogenic porcine reproductive and respiratory syndrome virus is 1: 1000; the maximum dilution multiple of the glutaraldehyde decamethyl ammonium bromide solution for 100% killing of the pseudorabies virus is 1: 500.

More preferably: the glutaraldehyde decamethyl ammonium bromide solution is used for dilution disinfection of different concentrations according to the use scene, and is used for wagon balance and piggery peripheral dilution multiple to be 1:2000, dilution factor of 1:1000 for use in pig farms and vehicles.

In conclusion, the invention has the following beneficial effects: the glutaraldehyde decamethylammonium bromide solution has no pungent smell, high stability, long-term storage and remarkable sterilization and disinfection effects under the low-temperature condition of (-35-0 ℃); can kill Escherichia coli, Staphylococcus aureus, Candida albicans, Bacillus subtilis, pathogenic blue ear virus, foot and mouth disease virus and H5N1 subtype avian influenza virus 100% at proper dilution. Is suitable for disinfection of farms, public places, equipment, instruments, hatching eggs and the like. The invention mainly has the following characteristics:

(1) the glutaraldehyde decamethylammonium bromide solution has high stability, does not generate layering phenomenon after long-term storage, and can keep high-efficiency sterilization and disinfection effects.

(3) The glutaraldehyde decamethylammonium bromide solution can kill Escherichia coli, staphylococcus aureus, Candida albicans, Bacillus subtilis, pathogenic porcine reproductive and respiratory syndrome virus and foot and mouth disease virus of pigs by 100 percent even at low temperature and under proper dilution times.

Detailed Description

The present invention will be described in detail with reference to examples.

In the following examples, glutaraldehyde, ammonium decamethyl bromide, higher fatty alcohol polyoxyethylene ether, and glycerol are commercially available.

Example 1: the glutaraldehyde decamethyl ammonium bromide solution is prepared from the following raw materials in percentage by weight: 6% of glutaraldehyde, 6% of decamethylammonium bromide, 0.3% of high-carbon fatty alcohol polyoxyethylene ether, 15% of glycerol and the balance of deionized water.

The preparation method of the glutaraldehyde decamethylammonium bromide solution comprises the following steps:

s1, weighing: weighing the raw material components according to the weight percentage of the raw material components of the glutaraldehyde decamethylammonium bromide solution;

s2, preparation of mixture A: mixing glutaraldehyde and decamethylammonium bromide, and stirring uniformly to obtain a mixture A;

s3, preparation of mixture B: adding fatty alcohol-polyoxyethylene ether and glycerol into 60ml of deionized water, then adding the mixture A, and stirring and uniformly mixing to obtain a mixture B;

s4, preparation of a glutaraldehyde decamethylammonium bromide solution: and (5) adjusting the pH value of the mixture B obtained in the step (S2) to 6, adding deionized water to a constant volume of 100ml, uniformly mixing, and subpackaging after passing inspection to obtain the glutaraldehyde decamethylammonium bromide solution.

Comparative example 1:

in the formulation of the glutaraldehyde decamethylammonium bromide solution, the difference from example 1 is that in comparative example 1, the higher fatty alcohol polyoxyethylene ether is removed, the amount of deionized water is increased, and the rest of the components and the preparation method refer to example 1.

Comparative example 2:

in the glutaraldehyde decamethylammonium bromide solution formulation, the difference from example 1 is that comparative example 2 is made by removing glycerin, and the rest of the components and the preparation method are referred to example 1.

Test example 1: stability test of glutaraldehyde decamethylammonium bromide solution

1. Test materials: the resulting glutaraldehyde decamethylammonium bromide solutions prepared in example 1 and comparative example 1.

2. The test method comprises the following steps: referring to disinfectant stability test in appendix of Chinese animal pharmacopoeia (2015 edition), the appearance, smell, pH value and content of glutaraldehyde decamethyl ammonium bromide solution are recorded after being placed for 5 and 10 days under the conditions of high temperature (60 ℃), illumination (4500 LX +/-500 LX) and low-temperature refrigeration (-15 ℃).

3. And (3) test results: see table 1.

TABLE 1 stability test of glutaraldehyde decamethylammonium bromide solutions

As can be seen from Table 1, the glutaraldehyde/decamethylammonium bromide solution prepared in example 1 of the present invention has substantially unchanged appearance, odor, pH, glutaraldehyde and decamethylammonium bromide content and high stability after being left for 10 days under high temperature, light and low temperature conditions. And the glutaraldehyde decamethyl ammonium bromide solution prepared in the comparative example 1 (fatty alcohol-free polyoxyethylene ether) is layered and turbid after being placed for 10 days under the conditions of high temperature and illumination, and the content of active ingredients is obviously reduced. The glutaraldehyde decamethylammonium bromide solution prepared in comparative example 2 (without glycerol) freezes at low temperature.

Test example 2: common germ kill test (carried out at-15 ℃ C. environment)

1. Test materials: glutaraldehyde decamethylammonium bromide solution prepared in example 1.

2. Test subjects: escherichia coli (ATCC8099), Staphylococcus aureus (ATCC6538), Candida albicans (ATCC10231), and Bacillus subtilis (ATCC 6633).

3. The test method comprises the following steps: the bactericidal properties were tested according to 2.1.11.3.1 in the Disinfection Specification (Ministry of health 2002). 0.1mL of each sample is taken and is dripped on a steel sheet with the diameter of 1.0cm, 20 mu L of bacterial liquid is respectively smeared on each steel sheet after the sample is dried, the steel sheets are respectively placed in a neutralizer tube for 2 min, 5min, 10min and 20min, and a viable count test is carried out after the neutralization for 10 min. The control group was prepared by directly dripping the bacterial liquid on a sterile steel plate, and the other steps were the same as those of the test group. The environmental temperature is 19.5-20 ℃, the bacterial culture temperature is 37 ℃, the result is observed after 48 hours of culture, and the test is repeated three times.

4. And (3) test results: see table 2.

TABLE 2 glutaraldehyde decamethylammonium bromide solution pathogen kill test

As can be seen from Table 2, the glutaraldehyde decamethylammonium bromide solution can effectively kill Escherichia coli, Staphylococcus aureus, Candida albicans and Bacillus subtilis, and when the dilution ratio of the aqueous solution is 1 (1000-2000), the Escherichia coli, Staphylococcus aureus and Candida albicans can be killed by 100%, and when the dilution ratio of the aqueous solution is 1:1000, the Bacillus subtilis can be killed by 100%. Therefore, the glutaraldehyde decamethylammonium bromide solution can kill escherichia coli, staphylococcus aureus, candida albicans and bacillus subtilis by 100% in a low-temperature environment (15 ℃ below zero).

Test example 3: killing test of highly pathogenic PRRSV and pseudorabies virus (PRV) with glutaraldehyde decamethylammonium bromide solution (performed at-15 deg.C)

1. Test materials: glutaraldehyde decamethylammonium bromide solution prepared in example 1.

2. Test subjects: highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), pseudorabies virus (PRV) and monkey kidney cell Marc-145, which are provided by animal epidemic disease prevention and control key open laboratories of Ministry of agriculture.

3. The test method comprises the following steps:

(1) monolayer cell culture: well-grown monkey kidney cells (Marc-145) and hamster kidney cells (BHK-21) were suspended in cell suspensions, 0.1mL each was placed in a 96-well cell culture plate, and the plate was placed in 5% CO₂In the incubator, after 24h, if the cells grow into a good monolayer, the cells are removed for use.

(2) Glutaraldehyde decamethylammonium bromide solution was tested for PRRSV kill: the method comprises the steps of diluting the PRRSV mixture solution by 100, 500 and 1000 times in example 1, mixing the diluted PRRSV mixture solution with virus suspension in a ratio of 9:1, performing action for 10, 30 and 60min respectively, then mixing the diluted PRRSV mixture solution with cell maintenance solution in a ratio of 1:9 respectively, performing gradient dilution by 10 times with the maintenance solution, inoculating the mixed solution of each dilution degree into a cell culture plate growing into a monolayer of cells once (discarding the original culture solution), namely inoculating the PRRSV mixture solution into Marc-145 cells, and inoculating the PRV mixture solution into BHK-21 cells. Each sample was inoculated into 4 wells, 0.1mL per well. A negative control was made by adding only 0.1mL of cell maintenance fluid to Marc-145 and BHK-21 cells. The PRRSV and PRV virus suspension is diluted by 10 times of gradient with cell maintenance liquid, then the virus suspension of each dilution is sequentially and correspondingly inoculated to a cell culture plate growing into a monolayer cell, namely PRRSV mixed liquid is inoculated to Marc-145 cells, and PRV mixed liquid is inoculated to BHK-21 cells. Each sample was inoculated into 4 wells, 0.1mL per well. Placing the inoculated culture plate at 37 deg.C and containing 5% CO₂The incubator is kept still and adsorbed for 1h, residual virus sample liquid is discarded after the virus is adsorbed in cells, 0.2mL of fresh cell maintenance liquid is added, and CO is put in₂The culture was continued in the incubator, and the growth of the cells was observed with an inverted microscope every day for 5-7 days continuously, and when the cells to be mutated did not develop the mutation and the control cells were intact, the results were counted and recorded, to obtain Table 3.

TABLE 3 killing of viruses by glutaraldehyde decamethylammonium bromide solution

As can be seen from Table 3, the highest dilution of 100% glutaraldehyde decamethylammonium bromide solution of the present invention against PRRSV is 1:1000 at-15 deg.C, but the killing time at 1:1000 cannot be less than 30 min. The highest dilution to 100% kill pseudorabies virus (PRV) was 1: 500.

Test example 4: killing tests (conducted at-15 ℃ C.) with glutaraldehyde decamethylammonium bromide solution on weighbridge, in piggery and on transport vehicles

Before the test, the dung is firstly cleaned, the pigsty and the ground of the wagon balance are washed by tap water, the floor of a hopper of a transport vehicle and the like, the pigsty is disinfected after being dried, and doors and windows are closed during the disinfection. After diluting the sample by 500, 1000, 2000 and 3000 times, the sample is taken after spraying for 15min, and the non-sterile area around the place is taken as a control. For each test point 3 samples were taken in parallel and the microorganisms were sampled by wiping.

And (3) performing gradient dilution on all samples by 10 times by using sterile physiological saline, then inoculating 0.1mL of sterile physiological saline to plate counting agar, placing the culture dish in a constant-temperature incubator at 37 ℃ for culturing for 18-24h, and counting each plate manually when the microbial colonies grow to the degree that the microbial colonies can be seen by naked eyes. Table 4 was obtained.

TABLE 4 Disinfection Effect of glutaraldehyde decamethylammonium bromide solution on weighbridge, in hog house and transport vehicles

As can be seen from Table 4, the optimal dilution factor for the glutaraldehyde decamethylammonium bromide solution of the present invention for a wagon balance at-15 ℃ is 1:2000, the optimal dilution multiple for piggery and vehicle is 1: 1000.

In conclusion, in the invention, the fatty alcohol-polyoxyethylene ether (high-carbon fatty alcohol-polyoxyethylene ether AEO) is a nonionic surfactant, has good performance at a temperature lower than the cloud point of the nonionic surfactant, and meets the requirement of low-temperature hard water resistance. The glutaraldehyde decamethylammonium bromide solution can be uniformly dispersed, the solution is more stable, and test example 1 can prove that the glutaraldehyde decamethylammonium bromide solution prepared by the method has no pungent smell, is still colorless clear liquid after being placed for 10 days under the conditions of high temperature, illumination and low temperature, keeps the content of effective components between 5.2 and 5.8 percent, and has high stability. More importantly, polyoxyethylene fatty alcohol ether is added into the glutaraldehyde to obviously improve the sterilization effect of the glutaraldehyde. It is possible that the polyoxyethylene fatty alcohol ether can reduce the volatilization of the effective components of glutaraldehyde and decamethylammonium bromide, and the mechanism is not clear. Test example 2 it can be confirmed that the sterilizing effect of the comparative example, in which the polyoxyethylene fatty alcohol ether is removed, is reduced with time.

Glycerol is a colorless, sweet, clear, viscous liquid, odorless, commonly known as glycerol. Glycerol is the earliest organic alcohol used for antifreeze and is replaced by methanol and ethylene glycol for cost reasons. With the more mature process, the price of the glycerol is greatly reduced. In addition, in practical application, the addition amount of the glycerol of 20 percent is equivalent to the antifreezing effect of the ethylene glycol or ethanol of 40 percent, so that the glycerol can replace the ethylene glycol or the ethanol in consideration of environmental protection and practical cost. The invention is matched with glycerol to reduce the freezing point of the disinfectant, the experimental examples 2-4 are all carried out at the temperature of-15 ℃, and the experimental example 2 can prove that the maximum dilution times of 100 percent of aqueous solutions for killing escherichia coli, staphylococcus aureus and candida albicans are 1:2000 and the maximum dilution times of 100 percent of aqueous solutions for killing bacillus subtilis are 1: 1000. Test example 3 can prove that the maximum dilution multiple of 100% glutaraldehyde decamethylammonium bromide solution for killing pathogenic porcine reproductive and respiratory syndrome virus is 1: 1000; the highest dilution factor for 100% killing of pseudorabies virus (PRV) was 1: 500. Test example 4 it can be demonstrated that the optimal dilution factor for loadlocks and pigsty peripheries is 1:2000, optimal dilution factor of 1:1000 for use in the pig farm and in the vehicle.

Example 2: the glutaraldehyde decamethylammonium bromide solution is different from the glutaraldehyde decamethylammonium bromide solution in the embodiment 1 in that the glutaraldehyde decamethylammonium bromide solution is prepared from the following raw materials in percentage by weight: 4% of glutaraldehyde, 4% of decamethylammonium bromide, 0.2% of high-carbon fatty alcohol polyoxyethylene ether, 6% of glycerol and the balance of deionized water. The glutaraldehyde decamethylammonium bromide solution was prepared in a manner different from that of example 1 in that, in step S4, the pH of mixture B was adjusted to 5.4.

Example 3: the glutaraldehyde decamethylammonium bromide solution is different from the glutaraldehyde decamethylammonium bromide solution in the embodiment 1 in that the glutaraldehyde decamethylammonium bromide solution is prepared from the following raw materials in percentage by weight: 7% of glutaraldehyde, 7% of decamethylammonium bromide, 2% of high-carbon fatty alcohol polyoxyethylene ether, 20% of glycerol and the balance of deionized water. The glutaraldehyde decamethylammonium bromide solution was prepared in a manner different from that of example 1 in that, in step S4, the pH of mixture B was adjusted to 6.6.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that several improvements and modifications without departing from the principle of the present invention will occur to those skilled in the art, and such improvements and modifications should also be construed as within the scope of the present invention.