阅读说明：本技术 表面修饰物及其制备方法和应用 (Surface modifier and preparation method and application thereof ) 是由 李文宇 于 2020-12-08 设计创作，主要内容包括：本发明公开了一种表面修饰物及其制备方法和应用,该表面修饰物的制备方法包括：在二硫键键合金刚烷修饰的聚乙二醇上接枝脂联素。通过该制备方法制备得到的表面修饰物(ADM)具有很好的稳定性,能够有效地减少细胞凋亡和促进前脂肪细胞增生个细胞迁移,从而减少脂肪积液的术后并发症,促进创伤修复,在脂肪组织创面愈合方面具有很好的应用前景。(The invention discloses a surface modifier and a preparation method and application thereof, wherein the preparation method of the surface modifier comprises the following steps: adiponectin is grafted on disulfide bond adamantane modified polyethylene glycol. The surface modifier (ADM) prepared by the preparation method has good stability, can effectively reduce apoptosis and promote cell migration of preadipocyte hyperplasia, thereby reducing postoperative complications of fat effusion, promoting wound repair and having good application prospect in the aspect of healing of fatty tissue wound surfaces.)

1. A method of preparing a surface finish, comprising: adiponectin is grafted on disulfide bond adamantane modified polyethylene glycol.

2. The method for producing a surface-modified substance according to claim 1, wherein the disulfide bond adamantane-modified polyethylene glycol is aminated and then the adiponectin is grafted;

preferably, the amination is maleimide-based functionalization.

3. The method for preparing a surface modifier according to claim 2, wherein the maleimide group functionalization is a reaction of disulfide bond-bonded adamantane-modified polyethylene glycol with succinimidyl 6- (maleimido) hexanoate, followed by separation and purification;

preferably, the mass ratio of the disulfide bond bonded adamantane modified polyethylene glycol to the 6- (maleimido) hexanoic acid succinimide ester is 2-3: 1-2;

preferably, the maleimide functionalization is carried out under stirring conditions, and the reaction time is at least 20 hours, more preferably 20-26 hours;

preferably, the separation and purification is separation and purification through a silica gel column.

4. The method of claim 2, wherein the step of grafting adiponectin comprises: reacting the aminated disulfide bond bonded adamantane modified polyethylene glycol with adiponectin in water, and dialyzing and purifying;

preferably, the mass ratio of the aminated disulfide bond adamantane-bonded polyethylene glycol to the adiponectin is 1-2: 1-2; the mass ratio of the adiponectin to the water is 1: 15-25;

preferably, the reaction for grafting adiponectin is performed under stirring conditions, and the reaction time is at least 40 hours, more preferably 40 to 60 hours, and still more preferably 48 hours.

5. The method for preparing the surface modifier according to any one of claims 1 to 4, wherein the disulfide bond adamantane-modified polyethylene glycol is prepared by the following steps:

reacting polyethylene glycol monomethyl ether and N, N-carbonyldiimidazole in an organic solvent, precipitating a reaction product by using a mixed solution of diethyl ether and tetrahydrofuran, standing, separating to obtain a solid, and then dripping an organic dissolved solution of the solid into a cystamine solution of triethylamine to perform a first reaction to obtain a polyethylene glycol derivative containing a disulfide bond;

reacting a disulfide bond-containing polyethylene glycol derivative with carbonyl imidazole (CDI), and then carrying out a secondary reaction with 1-adamantanecarboxylic acid to obtain disulfide bond-bonded adamantane-modified polyethylene glycol;

preferably, the product is purified after both the first and second reactions, more preferably by dialysis against a dialysis membrane.

6. A surface-modified product obtained by the production method according to any one of claims 1 to 5.

7. Use of a surface modification according to claim 6 in the manufacture of a medicament for promoting wound repair.

8. Use of a surface modification according to claim 6 in the manufacture of a medicament for promoting healing of a wound of adipose tissue.

9. Use of a surface modification according to claim 6 in the manufacture of a medicament for promoting the growth of preadipocytes.

10. A wound repair patch characterized in that the surface thereof in contact with the wound opening is coated with a wound repair drug comprising the surface modification of claim 6.

Technical Field

The invention relates to the technical field of medicines, in particular to a surface modifier and a preparation method and application thereof.

Background

Statistically, approximately 110 million people in the united states suffer acute trauma each year, and approximately 30 million hospitalizations. Incision complications are the most common postoperative complications of abdominal surgery, with an incidence of about 7% -26%. Common types of incision complications include: liquefaction of fat, incision fluid/seroma, incision dehiscence, incision infection, etc.

Among them, liquefaction of fat is the most common complication of incisions, and is better in obese patients. Meanwhile, the chronic refractory wounds caused by diabetes, paraplegia, local ray irradiation and the like are increased correspondingly. With the social progress, the requirements of people on beauty treatment are gradually increased, the level and quality of wound repair cannot meet the requirements of patients at present, and the contradiction between the level and the quality is acute.

Therefore, there is a need for drugs that are effective in promoting wound repair, particularly for promoting healing of adipose tissue wounds from surgical incisions.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The object of the present invention is to provide a surface modification, a method for its preparation and its use, which ameliorate at least one of the above mentioned technical problems.

The invention is realized by the following steps:

in a first aspect, the present invention provides a method for preparing a surface modification, comprising: adiponectin is grafted on disulfide bond adamantane modified polyethylene glycol.

Optionally, the disulfide-bonded adamantane-modified polyethylene glycol is aminated first, followed by grafting of the adiponectin.

Optionally, the amination is maleimide-based functionalization.

Alternatively, maleimide functionalization is to react the disulfide-bonded adamantane-modified polyethylene glycol with succinimidyl 6- (maleimide) hexanoate, followed by isolation and purification.

Optionally, the mass ratio of the disulfide bond adamantane-modified polyethylene glycol to the 6- (maleimido) hexanoic acid succinimide ester is 2-3: 1-2.

Optionally, the maleimide functionalization is carried out under stirring conditions, and the reaction time is at least 20 hours, more preferably 20 to 26 hours.

Alternatively, the separation and purification is separation and purification by a silica gel column.

Optionally, the step of grafting adiponectin comprises: and reacting the aminated disulfide bond-adamantane-modified polyethylene glycol with adiponectin in water, and dialyzing and purifying.

Optionally, the mass ratio of the aminated disulfide bond adamantane-modified polyethylene glycol to the adiponectin is 1-2: 1-2; the mass ratio of the adiponectin to the water is 1: 15-25.

Optionally, the reaction for grafting adiponectin is performed under stirring conditions, and the reaction time is at least 40 hours, more preferably 40 to 60 hours, and still more preferably 48 hours.

Alternatively, the disulfide bond adamantane modified polyethylene glycol is mainly prepared by the following steps:

reacting polyethylene glycol monomethyl ether and N, N-carbonyldiimidazole in an organic solvent, precipitating a reaction product by using a mixed solution of diethyl ether and tetrahydrofuran, standing, separating to obtain a solid, and then dripping an organic dissolved solution of the solid into a cystamine solution of triethylamine to perform a first reaction to obtain the polyethylene glycol derivative containing the disulfide bond. And (3) reacting the disulfide bond-containing polyethylene glycol derivative with carbonyl imidazole (CDI), and then carrying out secondary reaction with 1-adamantanecarboxylic acid to obtain the disulfide bond-bonded adamantane-modified polyethylene glycol.

Optionally, after both the first and second reactions, the product is purified, more preferably by dialysis against a dialysis membrane.

In a second aspect, the present invention also provides a surface modifier, which is prepared by the above preparation method.

In a third aspect, the invention also provides the application of the surface modifier in preparing a medicament for promoting wound repair.

In a fourth aspect, the invention also provides application of the surface modifier in preparation of a medicament for promoting healing of a wound surface of adipose tissue.

In a fifth aspect, the invention also provides the use of the surface modifier in the preparation of a medicament for promoting the growth of preadipocytes.

In a sixth aspect, the invention also provides a wound repairing plaster, wherein the surface of the wound repairing plaster, which is in contact with the wound opening, is coated with a wound repairing medicament, and the wound repairing medicament comprises the surface modifier.

The invention has the following beneficial effects: the surface modifier obtained by combining the polyethylene glycol modified by disulfide bond adamantane and adiponectin can effectively reduce apoptosis and promote proliferation of preadipocytes and migration of cells, thereby reducing postoperative complications of fatty effusion and promoting wound repair, and has good stability.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a graph showing absorbance (OD) values at 490nm measured by a microplate reader in an MTT cell proliferation assay in example 2 of the present invention;

FIG. 2 shows scratch repair experimental nodes of example 3 of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The following provides a specific description of the surface modifier, its preparation method and application.

Adiponectin is an endogenous bioactive polypeptide secreted by adipocytes and has been found to promote M2-type differentiation of macrophages by activating AMPK and PPAR γ, and has a function of regulating inflammatory responses. The inventors have creatively found through research and practice that the combination of polyethylene glycol modified by bonding adamantane through disulfide bond and adiponectin can ensure the stability of adiponectin and has a good effect on wound repair, and further propose the following scheme.

Some embodiments of the present invention provide a method of making a surface modification, comprising: adiponectin (GAD) was grafted onto disulfide-bonded adamantane-modified polyethylene glycol (AD-SS-PEG).

The surface modifier can well act with a tissue wound surface with a wound, effectively reduces apoptosis and promotes the migration of preadipocyte hyperplasia cells, thereby reducing postoperative complications of fat effusion and promoting wound repair, and the surface modifier has good stability and can continuously act.

Specifically, in some embodiments, the grafting process comprises: firstly, the disulfide bond-adamantane modified polyethylene glycol is aminated, and then the adiponectin is grafted.

It should be noted that other means capable of grafting adiponectin to disulfide bond adamantane-modified polyethylene glycol, i.e., grafting adiponectin after modification with a group other than an amino group, are also within the scope of the present invention.

In some embodiments, the amination includes, but is not limited to, maleimide functionalization.

Further, maleimide functionalization is to react the polyethylene glycol modified by disulfide bond adamantane with 6- (maleimide) hexanoic acid succinimidyl ester, and then to separate and purify, so as to obtain the maleimide functionalized AD-SS-PEG, namely AD-SS-PEG-Mal.

Wherein the mass ratio of the disulfide bond adamantane-bonded polyethylene glycol to the 6- (maleimido) hexanoic acid succinimide ester can be 2-3: 1-2, for example, 2: 1.8.

in some embodiments, the maleimide functionalization is performed under stirring conditions for a reaction time of at least 20 hours, more preferably 20 to 26 hours, for example, 24 hours. Wherein, the stirring reaction process is carried out at room temperature.

In some embodiments, the above separation and purification is separation and purification by silica gel column.

Further, the step of grafting the adiponectin comprises: the aminated disulfide-bonded adamantane-modified polyethylene glycol (e.g., AD-SS-PEG-Mal) is reacted with adiponectin in water, followed by dialysis purification.

Wherein the mass ratio of the aminated disulfide bond adamantane-bonded polyethylene glycol to adiponectin is 1-2: 1-2, such as 1: 1; the mass ratio of the adiponectin to the water is 1: 15-25, for example 1: 20.

In some embodiments, the grafting of adiponectin is performed under stirring for at least 40 hours, more preferably 40 to 60 hours, and even more preferably 48 hours.

Further, the disulfide bond-bonded adamantane-modified polyethylene glycol in the above embodiment is mainly prepared by the following steps:

s1, reacting polyethylene glycol monomethyl ether and N, N-carbonyldiimidazole in an organic solvent, precipitating the reaction product with a mixed solution of diethyl ether and tetrahydrofuran, standing, separating to obtain a solid, and dripping the solid organic solution into a cystamine solution of triethylamine to perform a first reaction to obtain a polyethylene glycol derivative (PEG-SS-CH) containing disulfide bonds₂CH₂NH₂)。

The organic solvent may be selected from dimethyl sulfoxide (DMSO), the reaction process of the first reaction may be performed under the protection of an inert gas, the reaction process is stirred, the reaction time may be 3 to 5 hours, preferably 4 hours, and the inert gas may be nitrogen. The standing process is carried out at 0 ℃, and the standing time can be 1.5-2.5 hours, preferably 2 hours. The solid organic solution is DMSO solution.

S2, mixingPEG-SS-CH₂CH₂NH₂Firstly reacting with carbonyl imidazole (CDI) and then carrying out secondary reaction with 1-adamantanecarboxylic acid to obtain disulfide bond bonded adamantane modified polyethylene glycol.

Wherein, the reaction of the second reaction is also carried out under the protection of inert gas, such as nitrogen. PEG-SS-CH₂CH₂NH₂The reaction time with carbonyl imidazole (CDI) was 4 hours, and the reaction time for the second reaction was also 4 hours.

In some embodiments, in order to make the purity of the subsequent product better and the pharmaceutical effect better, the product is purified after the first reaction and the second reaction, and more preferably, the purification is performed by dialysis through a dialysis membrane. The dialysis membrane can be MWCO of 2000.

Some embodiments of the present invention also provide a surface modifier prepared by the preparation method of any one of the above embodiments.

Some embodiments of the invention also provide the use of the surface modifier described above in the manufacture of a medicament for promoting wound repair.

Some embodiments of the invention also provide application of the surface modifier in preparing a medicament for promoting healing of a wound surface of adipose tissue.

Some embodiments of the invention also provide the use of the surface modifier described above in the preparation of a medicament for promoting the growth of preadipocytes.

Some embodiments of the invention also provide the use of the above surface modifier in the preparation of a medicament for promoting migration and survival of preadipocytes.

Some embodiments of the present invention also provide a wound repair patch, the side of which in contact with the wound opening is coated with a wound repair drug, the wound repair drug comprising the above surface modification.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The embodiment provides a preparation method of a surface modifier, which specifically comprises the following steps:

5.0g mPEG (1.0mmol) and 1.0g CDI (6.2mmol) were dissolved in 20ml DMSO and reacted under nitrogen with stirring for 4 h. The reaction product was precipitated with 1L of diethyl ether/tetrahydrofuran (volume ratio 4:1) and left to stand at 0 ℃ for 2 h. Dissolving the product after suction filtration in 20mL of LDMSO, then dropwise adding 2.2g of cystamine solution dissolved in 3mL of triethylamine, stirring and reacting for 12h, then dialyzing for 30h by using a dialysis membrane with MWCO of 2000, and freeze-drying to obtain the PEG derivative (PEG-SS-CH) containing the disulfide bond₂CH₂NH₂)2.8 g. 0.18g of 1-adamantanecarboxylic acid is dissolved in 20mL of anhydrous dichloromethane, 0.16g of CDI is added, and the mixture is reacted for 4 hours under the protection of nitrogen. Then 1.1g of PEG-SS-CH was added₂CH₂NH₂The reaction was continued for 4 h. The purification method is the same as PEG-SS-CH₂CH₂NH₂Purifying the product, and freeze-drying to obtain AD-SS-PEG for later use. By passing¹H NMR analysis showed that delta 3.6ppm was-CH in PEG₂CH₂The proton peak corresponding to O-is-SS-CH in delta 2.7-3.0 ppm₂The proton peak of-delta 1.5-1.9ppm is the proton peak in adamantane, indicating that AD-SS-PEG is successfully synthesized.

Stirring 2g of AD-SS-PEG and 1.8g of 6- (maleimide) hexanoic acid succinimidyl ester for 24h at room temperature, separating and purifying by a silica gel column after reaction to obtain maleimide functionalized AD-SS-PEG, then stirring 1g of AD-SS-PEG-Mal and 1g of adiponectin and 20mL of water for reaction for 48h, dialyzing and purifying to obtain disulfide bond bonded adamantane modified polyethylene glycol-adiponectin (AD-SS-PEG-GAD). By passing¹H NMR analysis showed that delta 3.6ppm was-CH in PEG₂CH₂The proton peak corresponding to O-is-SS-CH in delta 2.7-3.0 ppm₂The proton peak of (E) -is the proton peak in adamantane, delta 1.5-1.9ppm is the proton peak of adiponectin amino acid residue NH, delta 3.9-4.7 is the proton peak of adiponectin amino acid residue alpha-H, which indicates the successful synthesis of AD-SS-PEG-GAD.

Example 2

The effect of AD-SS-PEG-GAD (ADM for short) prepared in example 1 on mouse preadipocyte proliferation activity was examined by MTT method.

The cultured preadipocyte cell line was seeded at 5 × 105 in 96-well plates and placedPlacing in 5% CO₂Culturing for 5h at 37 ℃, after the cells are attached to the wall, randomly dividing the cells into 5 groups, adding ADM 0ng/mL (blank group), 0.5ng/mL, 1ng/mL, 5ng/mL and 10ng/mL (corresponding to the content of adiponectin) into 6 multiple wells in each group, and then continuously culturing for 72 h. The medium was aspirated, 20. mu.l MTT solution (5mg/mL) was added to each well, and incubation was continued for 4 h. The absorbance (OD) at 490nm was measured using a microplate reader, and the results are shown in FIG. 1.

As a result, the OD averages of 6 wells at 0, 0.5, 1, 5, and 10ng/mL were found to be 0.2, 0.24, 0.27, 0.25, and 0.25, respectively. It was shown that the growth of preadipocytes was promoted, and the corresponding promotion effect was the best at 1 ng/mL.

Example 3

According to 5 x 10⁵Preadipocyte cell lines were seeded in 6-well plates and placed in 5% CO₂And culturing at 37 ℃ for 24 h. When the degree of fusion reached 80%, a scratch was made with a 20. mu.l pipette tip, and the exfoliated cell layer was removed by washing 3 times with PBS buffer. Randomly divided into 2 groups, added to group a: group B: 10% DMEM medium (10% FBS); group B: 10% DMEM medium (containing 10% FBS) and 20. mu.g/ml ADM (corresponding to adiponectin content). The healing of the scratched cells at 0, 6, 12 and 24h was recorded, and the results are shown in FIG. 2. It should be noted that ADM used in this example was prepared from example 1.

The results show that the group A migrates in 6h and 12h, but undergoes apoptosis in 24h, but the group B (containing the surface modifier for promoting wound repair) undergoes cell migration in 6-24h and is completely healed in 24h, which indicates that the surface modifier for promoting wound repair (ADM) can effectively promote cell migration and is beneficial to healing of adipose tissue wounds.

Example 4

50Kg of Bama pigs were used. Taking the prone position, disinfecting the drape after vein induction anesthesia succeeds. The midline of the back is a symmetrical line, 3cm is laterally opened, 7 incisions with a length of about 4cm are respectively taken at the left and right sides, and adipose tissues are incised in a whole layer. Both incisions were randomly divided into ADM priming and control groups. One incision is opened every day after the operation (3 days after the operation and 5 days after the operation), local adipose tissues are reserved, and the healing grade of the adipose tissue incision is observed. As a result, the healing grade of the incision in the control group I was found to be significantly worse than that of the ADM intervention group, and the results are shown in the following table.

	Stage I incision healing Rate	Incidence of fat liquefaction	Incidence of infection in incisions
				ADM intervention group	95％	6％	0
Control group	60％	30％	7％

It should be noted that ADM used in this example was prepared from example 1.

In conclusion, MTT experiments and in vitro wound healing experiments verify that the surface modifier provided by the embodiment of the invention can promote proliferation and migration of preadipocytes, inhibit apoptosis and promote proliferation and migration of preadipocytes, so that postoperative complications of fatty effusion are reduced, and wound repair is promoted.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.