阅读说明：本技术 核酸构建体 (Nucleic acid constructs ) 是由 S.科多巴 J.西尔利伯恩 M.普勒 于 2019-07-31 设计创作，主要内容包括：本发明提供了核酸构建体,其包含：第一目的核苷酸序列(NOI1)；框滑动基序或翻译连读基序(FSM/TRM)；和第二目的核苷酸序列(NOI2)。本发明还提供了表达此类构建体的载体和细胞。本发明还提供了调节核酸构建体中两个转基因的相对表达的方法,该方法包括以下步骤：在两个转基因之间包括框滑动基序或翻译连读基序,以减少下游转基因的表达。(The present invention provides a nucleic acid construct comprising: a first nucleotide sequence of interest (NOI 1); a frame sliding motif or a translation read-through motif (FSM/TRM); and a second nucleotide sequence of interest (NOI 2). The invention also provides vectors and cells expressing such constructs. The invention also provides a method of modulating the relative expression of two transgenes in a nucleic acid construct, the method comprising the steps of: a frame sliding motif or a translation read-through motif is included between the two transgenes to reduce expression of the downstream transgene.)