阅读说明：本技术 基于成像端扫描的显微成像光谱仪设计方法 (Microscopic imaging spectrometer design method based on imaging end scanning ) 是由 唐义 常月娥 李平安 刘婉玉 于 2019-04-29 设计创作，主要内容包括：本发明提出基于成像端扫描的显微成像光谱仪设计方法,该方法改进了传统物方扫描数据获取方法,采用对经显微镜放大后的像方进行扫描的成像端扫描数据获取方法,和传统的物方扫描方式相比,成像端像方扫描方式对扫描移动装置的精度及重复性要求降低了几十甚至上百倍,极大提高了扫描成像光谱数据的成像质量,同时降低了显微成像光谱系统的成本。(The invention provides a design method of a microscopic imaging spectrometer based on imaging end scanning, which improves the traditional object space scanning data acquisition method, adopts the imaging end scanning data acquisition method for scanning an image space amplified by a microscope, and compared with the traditional object space scanning mode, the imaging end image space scanning mode reduces the requirements on the precision and the repeatability of a scanning moving device by dozens of times or even hundreds of times, greatly improves the imaging quality of scanning imaging spectrum data, and simultaneously reduces the cost of a microscopic imaging spectrum system.)

1. The design method of the microscopic imaging spectrometer based on the imaging end scanning is characterized in that hyperspectral imaging data scanning and acquisition are carried out on an image space amplified by a microscope.

2. The design method of the microscopic imaging spectrometer based on the imaging end scanning is characterized in that the imaging spectrometer adopts a slit push-broom dispersion system.

3. The design method of the microscopic imaging spectrometer based on the imaging end scanning is characterized by comprising 3 push-scanning systems.

Scanning system 1: a whole machine translation or rotation mode push-broom scanning system;

scanning system 2: a mirror-sweeping scanning mode push-sweeping scanning system;

scanning system 3: a slit moving mode push-scan system.

4. The method as claimed in claim 4, wherein the whole-machine translation or rotation mode push-scan system uses a translation stage/rotation stage to translate/rotate the whole imaging spectrometer, and the lens of the imaging spectrometer is coupled and aligned with the side light outlet of the microscope, so as to scan and collect data of the target amplified image output by the side light outlet of the imaging spectrometer.

5. The method according to claim 4, wherein the scanning mirror scanning mode push-scan scanning system adds a scanning mirror as an optical path coupling device between the imaging spectrometer and the light outlet on the microscope side, and data scanning acquisition of the target magnified image output by the light outlet on the microscope side by the imaging spectrometer is realized through scanning of the scanning mirror.

6. The method according to claim 4, wherein the slit moving mode push-scan scanning system realizes data scanning and acquisition of the target magnified image output from the light outlet on the microscope side by controlling the slit moving by using a motor.

Technical Field

The invention relates to a design method of an imaging spectrometer based on imaging end scanning, which is matched with a microscope for use, the imaging spectrometer carries out scanning imaging on an imaging target amplified by the microscope, improves a common scanning method for an object without amplification, reduces the dependence on the movement precision of a scanning mechanism, and greatly improves the imaging quality of acquired hyperspectral imaging data.

Background

The imaging spectrum technology has excellent spectrum imaging information acquisition capability, and the push-broom slit mechanism scanning imaging is used as a common data acquisition mode, can acquire the spectrum information of a target object and also can acquire the spatial distribution information of a target component, and becomes a powerful means of an optical detection technology. Meanwhile, high requirements are put forward on the micro-imaging spectrum technology due to the acquisition requirements of micro-target spectrum imaging information in the fields of medicine, industry, agriculture, food, physics, chemistry, geology, minerals, pollution, photoelectric detection and the like.

The scanning mode of the prior common microscopic hyperspectral imaging technology is object space scanning, namely an object translation stage carrying a tiny target of a translation microscope, the method has very high requirements on indexes such as translation precision, translation repeatability and the like of the object translation stage, and often cannot meet the use requirement, so that the imaging quality such as the imaging resolution precision, the scanning dimension and image quality uniformity and the like of a microscopic imaging spectrometer are seriously limited by the object translation stage, and meanwhile, the price of a microscopic imaging spectrum system is greatly improved by using the object translation stage.

Disclosure of Invention

In view of the above, a method for designing a microscopic imaging spectrometer based on imaging end scanning is provided, which improves the conventional object space scanning data acquisition method, and adopts an imaging end scanning data acquisition method for scanning an image space amplified by a microscope.

The technical scheme for realizing the invention is as follows:

the design method of the microscopic imaging spectrometer based on the scanning of the imaging end is used for scanning and collecting hyperspectral imaging data of an image space amplified by a microscope;

The design method of the microscopic imaging spectrometer based on the imaging end scanning comprises the steps that the imaging spectrometer adopts a slit push-broom system;

the design method of the microscopic imaging spectrometer based on the scanning of the imaging end comprises the following scanning modes:

(1) overall translation (see fig. 1) or rotation mode (see fig. 2): the whole imaging spectrometer is translated/rotated by using the translation table/rotation table, and the lens of the imaging spectrometer is coupled and aligned with the side light outlet of the microscope, so that the data scanning and acquisition of the target amplified image output by the side light outlet of the imaging spectrometer are realized.

(2) Scanning mode of the mirror (see fig. 3): namely, the scanning mirror is used as a coupling device between the imaging spectrometer and the light outlet at the microscope side, and the scanning of the scanning mirror realizes the data scanning and acquisition of the target amplified image output by the imaging spectrometer to the light outlet at the microscope side.

(3) Slit movement pattern (see fig. 4): the method realizes the data scanning and acquisition of the target amplified image output by the light outlet at the microscope side by using a motor to control the movement of the slit.

The design method of the microscopic imaging spectrometer based on the imaging end scanning comprises the following scanning and collecting processes:

(1) placing the target on a microscope objective table, carrying out amplified imaging on the target by a microscope, and outputting the imaged image through a side light outlet;

(2) A lens of the imaging spectrometer is coupled and aligned with a side light outlet of the microscope, and receives target amplified imaging data output by the side light outlet;

(3) the scanning imaging of the slit spectrum data of the imaging spectrometer is realized by utilizing a scanning imaging mechanism, and the scanning mechanism is divided into the following types:

a scanning mechanism for translating/rotating the whole imaging spectrometer by utilizing a translation table/rotating table loaded below the whole imaging spectrometer;

loading a scanning mirror between a lens of the imaging spectrometer and a light outlet at the side of the microscope, and utilizing a scanning mechanism of rotation of the scanning mirror;

a motor which moves in a direction vertical to a slit is loaded on the slit behind a lens of an imaging spectrometer, so that a scanning mechanism is realized.

Has the advantages that:

compared with the traditional object space scanning data acquisition method, the method has the following advantages:

(1) imaging the amplified target image, and expanding the precision of the data acquisition spatial resolution by dozens of times;

(2) by carrying out imaging spectrum scanning on the amplified target image, the method reduces the requirement on the motor movement precision by dozens of or even hundreds of times, greatly reduces the requirement on the motor movement precision, and is easier to realize;

(3) the traditional object space scanning method requires that the moving precision of the motor is micron or below, so the manufacturing cost is very high, and the method reduces the moving precision requirement of the motor by dozens of or even hundreds of times, thereby greatly saving the cost of the microscopic imaging spectrum system.

Drawings

FIG. 1 is a schematic diagram of the operation of a whole machine translation mode imaging end scanning micro-imaging spectrometer according to the present invention.

FIG. 2 is a schematic diagram of the operation of the whole machine rotation mode imaging end scanning micro-imaging spectrometer of the present invention.

FIG. 3 is a schematic diagram of the operation of a scanning microscope imaging spectrometer of the present invention.

FIG. 4 is a schematic diagram of the operation of a slit moving mode imaging end scanning micro-imaging spectrometer according to the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings in conjunction with specific examples.

The invention provides a design method of a microscopic imaging spectrometer based on imaging end scanning, which improves the traditional object space scanning data acquisition method, adopts the imaging end scanning data acquisition method for scanning an image space amplified by a microscope, and compared with the traditional object space scanning mode, the imaging end image space scanning mode reduces the requirements on the precision and the repeatability of a scanning moving device by dozens of times or even hundreds of times, greatly improves the imaging quality of scanning imaging spectrum data, and simultaneously reduces the cost of a microscopic imaging spectrum system.

The design method of the imaging end scanning microscopic imaging spectrometer adopts a slit push-broom system to scan and collect hyperspectral imaging data of an image space amplified by a microscope, has three scanning and collecting modes, and describes the implementation modes of the scanning and collecting modes respectively.