阅读说明：本技术 一种低敏乳蛋白功能因子组合物其应用和一种低敏儿童配方奶粉 (Hypoallergenic lactoprotein functional factor composition, application thereof and hypoallergenic child formula milk powder ) 是由 楼佳佳 李归浦 姜艳喜 华家才 储小军 孔迎 于 2020-07-31 设计创作，主要内容包括：本发明提供了一种低敏乳蛋白功能因子组合物,包括：水解乳清蛋白粉750～815质量份；低聚果糖粉90～185质量份；动物双歧杆菌Bb-12冻干粉0.6～1.8质量份。本发明通过对水解乳清蛋白粉的水解度、分子量分布和致敏抗原活性的研究和动物过敏试验开发出低致敏效果明显的乳蛋白功能因子组合物(水解乳清蛋白+低聚果糖+动物双歧杆菌Bb-12)的特定组分和特定复配比例。其中,蛋白质由水解乳清蛋白粉提供,最大限度地降低了产品中的乳蛋白致敏性,同时添加益生元(低聚果糖)和益生菌(动物双歧杆菌Bb-12),显著提高了儿童的抗乳蛋白过敏能力,适合乳蛋白过敏儿童食用以避免乳蛋白过敏。(The invention provides a hypoallergenic milk protein functional factor composition, which comprises the following components in part by weight: 750-815 parts by mass of hydrolyzed whey protein powder; 90-185 parts by mass of fructo-oligosaccharide powder; 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder. The invention develops the specific components and the specific compounding proportion of the milk protein functional factor composition (the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12) with obvious hypoallergenic effect by researching the hydrolysis degree, the molecular weight distribution and the allergenic antigen activity of the hydrolyzed whey protein powder and animal allergy tests. The protein is provided by the hydrolyzed whey protein powder, so that the allergenicity of the milk protein in the product is reduced to the maximum extent, and meanwhile, the prebiotics (fructooligosaccharides) and the probiotics (bifidobacterium animalis Bb-12) are added, so that the milk protein allergy resistance of children is remarkably improved, and the milk protein allergy-free milk protein powder is suitable for being eaten by children with milk protein allergy to avoid milk protein allergy.)

1. A hypoallergenic milk protein functional factor composition comprising:

750-815 parts by mass of hydrolyzed whey protein powder; 90-185 parts by mass of fructo-oligosaccharide powder; 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder.

2. The composition as claimed in claim 1, wherein the content of the hydrolyzed whey protein in the hydrolyzed whey protein powder is not less than 80%, the content of the fructo-oligosaccharide in the fructo-oligosaccharide powder is not less than 90%, and the content of the bifidobacterium animalis Bb-12 in the lyophilized powder of the bifidobacterium animalis Bb-12 is 2.0-4.0 x 10¹⁰CFU/g。

3. The composition as claimed in claim 1, wherein the hydrolyzed whey protein powder has a degree of proteolysis of 15.0-22.0%, and a molecular weight distribution of protein specifically:

the mass percentage of the fragments with the molecular weight of 500-2000 Da is 30-35%, the mass percentage of the fragments with the molecular weight of 200-500 Da is 25-30%, and the mass percentage of the fragments with the molecular weight less than 200Da is 3-6%.

4. The composition according to claim 1, wherein the sensitizing antigenic activity of the hydrolyzed whey protein powder is specifically: the antigenicity reduction rate of alpha-lactalbumin is more than 93 percent, and the antigenicity reduction rate of beta-lactoglobulin is more than 55 percent.

5. The composition according to claim 1, wherein the hypoallergenic milk protein functional factor composition further comprises 0-160 parts by mass of an auxiliary material, and the auxiliary material is selected from lactose, maltodextrin or glucose syrup dry powder.

6. The composition of claim 1, wherein the hypoallergenic milk protein functional factor composition comprises:

760-810 parts by mass of hydrolyzed whey protein powder; 100-180 parts by mass of fructo-oligosaccharide powder; 0.8-1.6 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder.

7. The composition according to claim 1, wherein in the hypoallergenic milk protein functional factor composition: the relative proportion of the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12 is 20g (3-5) g:1 × 10⁹CFU。

8. Use of the hypoallergenic milk protein functional factor composition according to any one of claims 1 to 7 for the preparation of a hypoallergenic children's formula.

9. A hypoallergenic children formula milk powder, characterized in that it comprises: the hypoallergenic milk protein functional factor composition according to any one of claims 1 to 7.

10. The hypoallergenic child formula according to claim 9, wherein said hypoallergenic child formula comprises:

250 to 450 parts by mass of the hypoallergenic milk protein functional factor composition according to any one of claims 1 to 7; 250-380 parts by mass of vegetable fat powder; 300-350 parts by mass of lactose; 4-6 parts of compound vitamin; 8-12 parts by mass of a composite mineral substance; 0-0.6 parts by mass of lactoferrin; 0-5 parts of arachidonic acid powder and 0-5 parts of docosahexaenoic acid powder.

Technical Field

The invention relates to the technical field of food, in particular to a hypoallergenic milk protein functional factor composition, application thereof and hypoallergenic children formula milk powder.

Background

Protein allergy caused by eating formula food with cow milk as milk base is one of the most common food allergy problems of children at present, and the incidence rate of cow milk protein allergy in children is reported to be 0.3-7.5%. Studies have shown that there are more than 20 proteins in cow's milk that may cause allergic reactions, of which casein, beta-lactoglobulin and alpha-lactalbumin are the most major allergens. Skin and gastrointestinal symptoms such as eczema, vomiting, diarrhea, constipation, non-pathological gastroesophageal reflux and the like are frequently caused when children patients are allergic, and the growth and development of the children are seriously influenced. In order to effectively improve the situation and ensure the healthy growth of children, on one hand, the allergenicity of cow milk protein needs to be improved, and on the other hand, the improvement of the allergenicity of cow milk protein is also important.

Cow's milk proteins cause allergy mainly by their allergen epitopes, i.e. the components of the allergen involved in binding antibodies. The protein enzymolysis technology can change the amino acid residues, polypeptide chains and protein macromolecule spatial structures of the milk protein, thereby achieving the purpose of masking or hiding the epitope of the protein allergen and finally obtaining the hypoallergenic milk protein. It is an effective method for reducing milk protein immunogenicity, and can improve gastrointestinal tolerance and reduce anaphylaxis.

In addition to the idea of reducing the allergenicity of cow's milk protein in solving the problem of cow's milk protein allergy in children, great attention has been paid to the research on the cow's milk protein allergy resistance in children, and improvement of intestinal microecology in children is considered to be one of the most effective ways to improve cow's milk protein allergy resistance in children. Many studies have shown that the intestinal flora of cow milk protein allergic children is greatly different from that of normal children, the numbers of conditional pathogenic bacteria escherichia coli and bacteroides are obviously increased, the numbers of probiotic bacteria bifidobacterium and lactobacillus are obviously reduced, and the dysbacteriosis of intestinal microecology is one of the main reasons for cow milk protein allergy. The probiotics and prebiotics can improve the antiallergic property of the children to the cow milk protein by improving the intestinal barrier function of the children allergic to the cow milk protein, adjusting the intestinal flora structure, correcting unbalanced Th1/Th2 cell balance and the like.

At present, the main strategy of domestic dairy products in solving the problem of cow's milk protein allergy of children is to reduce the allergenicity of cow's milk protein or improve the anti-allergy of children to cow's milk protein, so that the development of the formula milk powder which can reduce the allergenicity of cow's milk protein in products and improve the anti-cow's milk protein allergy ability of children is very necessary.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide a hypoallergenic milk protein functional factor composition, which can improve the milk protein allergy resistance of children and simultaneously has low milk protein allergenicity in products.

The invention provides a hypoallergenic milk protein functional factor composition, which comprises the following components in part by weight:

750-815 parts by mass of hydrolyzed whey protein powder; 90-185 parts by mass of fructo-oligosaccharide powder; 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder.

Preferably, the content of the hydrolyzed whey protein in the hydrolyzed whey protein powder is more than or equal to 80 percent;

preferably, the protein hydrolysis degree of the hydrolyzed whey protein powder is 15.0-22.0%;

preferably, the protein molecular weight distribution of the hydrolyzed whey protein powder is specifically as follows:

the mass percentage of the fragments with the molecular weight of 500-2000 Da is 30-35%, the mass percentage of the fragments with the molecular weight of 200-500 Da is 25-30%, and the mass percentage of the fragments with the molecular weight less than 200Da is 3-6%.

Preferably, the sensitization antigen activity of the hydrolyzed whey protein powder is specifically as follows: the antigenicity reduction rate of alpha-lactalbumin is more than 93 percent, and the antigenicity reduction rate of beta-lactoglobulin is more than 55 percent.

Preferably, the content of the fructo-oligosaccharide in the fructo-oligosaccharide powder is more than or equal to 90 percent;

preferably, the content of the bifidobacterium animalis Bb-12 in the bifidobacterium animalis Bb-12 freeze-dried powder is 2.0-4.0 x 10¹⁰CFU/g。

Preferably, the hypoallergenic milk protein functional factor composition further comprises 0-160 parts by mass of an auxiliary material, and the auxiliary material is selected from lactose, maltodextrin or glucose syrup dry powder.

Preferably, the hypoallergenic milk protein functional factor composition comprises:

760-810 parts by mass of hydrolyzed whey protein powder; 100-180 parts by mass of fructo-oligosaccharide powder; 0.8-1.6 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder;

preferably, in the hypoallergenic milk protein functional factor composition: the relative proportion of the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12 is 20g (3-5) g:1 × 10⁹CFU。

The invention provides application of the hypoallergenic milk protein functional factor composition in any one of the technical schemes in preparation of hypoallergenic children formula milk powder.

The invention provides hypoallergenic children formula milk powder, which comprises the following components in parts by weight: the hypoallergenic milk protein functional factor composition according to any one of the above technical schemes.

Preferably, the hypoallergenic children formula comprises:

250-450 parts by mass of the hypoallergenic milk protein functional factor composition in any one of the above technical schemes; 250-380 parts by mass of vegetable fat powder; 300-350 parts by mass of lactose; 4-6 parts of compound vitamin; 8-12 parts by mass of a composite mineral substance; 0-0.6 parts by mass of lactoferrin; 0-5 parts of arachidonic acid powder and 0-5 parts of docosahexaenoic acid powder.

Compared with the prior art, the invention provides a hypoallergenic milk protein functional factor composition, which comprises the following components in percentage by weight: 750-815 parts by mass of hydrolyzed whey protein powder; 90-185 parts by mass of fructo-oligosaccharide powder; 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder. The invention develops the specific components and the specific compounding proportion of the milk protein functional factor composition (the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12) with obvious hypoallergenic effect by researching the hydrolysis degree, the molecular weight distribution and the allergenic antigen activity of the hydrolyzed whey protein powder and animal allergy tests. The protein is provided by the hydrolyzed whey protein powder, so that the allergenicity of the milk protein in the product is reduced to the maximum extent, and meanwhile, the prebiotics (fructooligosaccharides) and the probiotics (bifidobacterium animalis Bb-12) are added, so that the milk protein allergy resistance of children is remarkably improved, and the milk protein allergy-free milk protein powder is suitable for being eaten by children with milk protein allergy to avoid milk protein allergy.

Drawings

FIG. 1 comparison of allergy symptom scores of different groups of BALB/c mice in example 6 of the present invention;

FIG. 2 comparison of serum IgE levels of BALB/c mice of different groups in example 6 of the present invention;

FIG. 3 comparison of plasma HIS levels in different groups of BALB/c mice in example 6 of the present invention;

FIG. 4 comparison of spleen T lymphocyte proliferation stimulation indexes of different groups of BALB/c mice in example 6 of the present invention;

FIG. 5 comparison of spleen B lymphocyte proliferation stimulation indexes of different groups of BALB/c mice in example 6 of the present invention.

Detailed Description

The invention provides a hypoallergenic milk protein functional factor composition, application thereof and hypoallergenic children formula milk powder, and a person skilled in the art can use the contents for reference and appropriately improve process parameters to realize the hypoallergenic children formula milk powder. It is expressly intended that all such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the scope of the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The invention provides a hypoallergenic milk protein functional factor composition, which comprises the following components in part by weight:

750-815 parts by mass of hydrolyzed whey protein powder; 90-185 parts by mass of fructo-oligosaccharide powder; 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder.

The invention provides a hypoallergenic milk protein functional factor composition which comprises 750-815 parts by mass of hydrolyzed whey protein powder; preferably comprises 760 to 810 parts by mass of hydrolyzed whey protein powder.

The invention has requirements on the hydrolyzed whey protein powder, and specifically comprises the following steps: the content of the hydrolyzed whey protein is more than or equal to 80 percent, and the degree of protein hydrolysis is 15.0 to 22.0 percent.

The preferable protein molecular weight distribution of the hydrolyzed whey protein powder is as follows:

the mass percentage of the fragments with the molecular weight of 500-2000 Da is 30-35%, the mass percentage of the fragments with the molecular weight of 200-500 Da is 25-30%, and the mass percentage of the fragments with the molecular weight less than 200Da is 3-6%.

More preferably:

the mass percentage of the fragments with the molecular weight of 500-2000 Da is 31-34%, the mass percentage of the fragments with the molecular weight of 200-500 Da is 25-29%, and the mass percentage of the fragments with the molecular weight less than 200Da is 3-6%. The total amount of the components is 100 percent.

The sensitization antigen activity of the hydrolyzed whey protein powder is specifically as follows: the antigenicity reduction rate of alpha-lactalbumin is more than 93 percent, and the antigenicity reduction rate of beta-lactoglobulin is more than 55 percent.

The inventor finds that only the hydrolyzed whey protein powder which meets the requirements on protein content, hydrolysis degree, molecular weight distribution and antigen activity can meet the requirements, and the technical effect of remarkably reducing the allergenicity of the milk protein in the product is achieved.

The hypoallergenic lactoprotein functional factor composition provided by the invention comprises 90-185 parts by mass of fructo-oligosaccharide powder; preferably comprises 100-180 parts by mass of fructo-oligosaccharide powder.

The content of the fructo-oligosaccharide in the fructo-oligosaccharide powder is more than or equal to 90 percent.

The source of the fructo-oligosaccharide powder is not limited in the invention, and the fructo-oligosaccharide powder is commercially available and well known to those skilled in the art.

The hypoallergenic lactoprotein functional factor composition provided by the invention comprises 0.6-1.8 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder; preferably comprises 0.8-1.6 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder.

The content of bifidobacterium animalis Bb-12 in the bifidobacterium animalis Bb-12 freeze-dried powder is 2.0-4.0 multiplied by 10¹⁰CFU/g。

The source of the bifidobacterium animalis Bb-12 freeze-dried powder is not limited in the invention, and the bifidobacterium animalis Bb-12 freeze-dried powder is commercially available and well known by a person skilled in the art.

The hypoallergenic milk protein functional factor composition also comprises 0-160 parts by mass of auxiliary materials, wherein the auxiliary materials are selected from lactose, maltodextrin or glucose syrup dry powder. The present invention is not limited in its source, and may be commercially available.

According to the present invention, the hypoallergenic milk protein functional factor composition comprises:

760-810 parts by mass of hydrolyzed whey protein powder; 100-180 parts by mass of fructo-oligosaccharide powder; 0.8-1.6 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder;

according to the invention, in the hypoallergenic milk protein functional factor composition: the relative proportion of the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12 is 20g (3-5) g:1 × 10⁹A CFU; specifically, the ratio of 20g to 3g to 1X 10⁹CFU；20g:4g:1×10⁹CFU；20g:5g:1×10⁹CFU。

The composition of the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12 which meet the proportion can improve the milk protein allergy resistance of children and simultaneously has low milk protein allergy in the product.

The method systematically evaluates and analyzes the raw material hydrolyzed whey protein powder through three factors of the hydrolysis degree, the molecular weight distribution and the allergenic antigen activity, thereby obtaining the relatively stable hydrolyzed whey protein powder raw material with the lowest allergenic antigen activity. The hypoallergenic milk protein functional factor compositions (hydrolyzed whey protein, fructo-oligosaccharide and bifidobacterium animalis Bb-12) with different proportions are subjected to sensitization effect study through animal allergy tests, so that the optimal proportion composition is obtained.

The invention also provides a preparation method of the hypoallergenic lactoprotein functional factor composition, which comprises the following steps:

mixing the hydrolyzed whey protein powder, the fructo-oligosaccharide powder and the bifidobacterium animalis Bb-12 freeze-dried powder.

The invention provides application of the hypoallergenic milk protein functional factor composition in any one of the technical schemes in preparation of hypoallergenic children formula milk powder.

The invention provides hypoallergenic children formula milk powder, which comprises the following components in parts by weight: the hypoallergenic milk protein functional factor composition according to any one of the above technical schemes.

The specific components and proportions of the hypoallergenic milk protein functional factor composition according to any one of the above technical schemes are clearly described in the invention, and are not repeated herein.

According to the invention, the hypoallergenic children formula milk powder comprises:

250-450 parts by mass of the hypoallergenic milk protein functional factor composition in any one of the above technical schemes; 250-380 parts by mass of vegetable fat powder; 300-350 parts by mass of lactose; 4-6 parts of compound vitamin; 8-12 parts by mass of a composite mineral substance; 0-0.6 parts by mass of lactoferrin; 0-5 parts of arachidonic acid powder and 0-5 parts of docosahexaenoic acid powder.

The hypoallergenic children's formula preferably comprises:

270-410 parts by mass of the hypoallergenic milk protein functional factor composition in any one of the technical schemes; 260-360 parts by mass of vegetable fat powder; 310-340 parts of lactose; 5 parts of compound vitamin; 9-11 parts by mass of a composite mineral substance; 0-0.6 parts by mass of lactoferrin; 0-5 parts of arachidonic acid powder and 0-5 parts of docosahexaenoic acid powder.

The sources of the components are not limited in the invention, and the components are available on the market.

The invention applies the milk protein functional factor composition (hydrolyzed whey protein, fructo-oligosaccharide and bifidobacterium animalis Bb-12) with obvious hypoallergenic effect to the production of the children formula milk powder.

The invention provides a hypoallergenic milk protein functional factor composition, which comprises the following components in part by weight: 760-810 parts by mass of hydrolyzed whey protein powder; 100-180 parts by mass of fructo-oligosaccharide powder; 0.8-1.6 parts by mass of bifidobacterium animalis Bb-12 freeze-dried powder. The invention develops the specific components and the specific compounding proportion of the milk protein functional factor composition (the hydrolyzed whey protein, the fructo-oligosaccharide and the bifidobacterium animalis Bb-12) with obvious hypoallergenic effect by researching the hydrolysis degree, the molecular weight distribution and the allergenic antigen activity of the hydrolyzed whey protein powder and animal allergy tests. The protein is provided by the hydrolyzed whey protein powder, so that the allergenicity of the milk protein in the product is reduced to the maximum extent, and meanwhile, the prebiotics (fructooligosaccharides) and the probiotics (bifidobacterium animalis Bb-12) are added, so that the milk protein allergy resistance of children is remarkably improved, and the milk protein allergy-free milk protein powder is suitable for being eaten by children with milk protein allergy to avoid milk protein allergy.

In order to further illustrate the present invention, the following will describe in detail a hypoallergenic milk protein functional factor composition, its use and a hypoallergenic children formula provided by the present invention with reference to the examples.