阅读说明：本技术 一种防龋齿棒棒糖及其制备方法 (Anti-caries lollipop and preparation method thereof ) 是由 周爱琴 周爱兵 张璐璐 于茂兰 于 2020-08-06 设计创作，主要内容包括：本发明属于食品加工技术领域,提供了一种防龋齿棒棒糖,所述防龋齿棒棒糖中添加葡萄糖基甜菊糖苷和甜菊糖苷作为甜味剂,包括如下重量份的组分：葡萄糖基甜菊糖苷0.1-5份、甜菊糖苷0.1-1份、异麦芽酮糖醇90-98份、0.005-0.05溶菌酶份和柠檬酸1-3份。本申请的棒棒糖以葡萄糖基甜菊糖苷和甜菊糖苷为主要甜味剂,甜味自然丰富,在口腔中不被口腔细菌所分解,不致龋齿,巩齿健齿；加入异麦芽酮糖醇,富含双歧杆菌增殖因子,促进双歧杆菌的生长繁殖,维持肠道的微生态平衡,有利于人体的健康。(The invention belongs to the technical field of food processing, and provides a lollipop for preventing dental caries, wherein glucosyl stevioside and stevioside serving as sweetening agents are added into the lollipop for preventing dental caries, and the lollipop comprises the following components in parts by weight: 0.1-5 parts of glucosyl stevioside, 0.1-1 part of stevioside, 90-98 parts of isomalt, 0.005-0.05 part of lysozyme and 1-3 parts of citric acid. The lollipop takes glucose-based stevioside and stevioside as main sweeteners, has natural and rich sweet taste, is not decomposed by oral bacteria in an oral cavity, does not cause decayed teeth, consolidates the teeth and strengthens the teeth; the isomaltitol is added, is rich in bifidobacterium proliferation factors, promotes the growth and the reproduction of the bifidobacterium, maintains the micro-ecological balance of intestinal tracts and is beneficial to the health of human bodies.)

1. The lollipop is characterized in that glucosyl stevioside and stevioside serving as sweetening agents are added into the lollipop for preventing the decayed teeth.

2. The anti-caries lollipop of claim 1, comprising the following components in parts by weight: 0.1-5 parts of glucosyl stevioside, 0.1-1 part of stevioside, 90-98 parts of isomalt, 0.005-0.05 part of lysozyme and 1-3 parts of citric acid.

3. The anti-caries lollipop of claim 1, wherein the glucosyl steviol glycoside is obtained by introducing glucosyl groups into steviol glycoside using cyclodextrin glucoside transferase.

4. The method for preparing a caries prevention lollipop according to claim 1, wherein the purity of the glucosyl steviol glycoside is at least 80%.

5. The anti-caries lollipop of claim 1, wherein the rebaudioside a content of the steviol glycosides is greater than 90 wt%.

6. The method for preparing a caries prevention lollipop according to any one of claims 1 to 5, comprising the steps of:

step S1: accurately weighing glucosyl stevioside, stevioside and isomaltitol, and uniformly mixing, wherein the mass ratio of the components is as follows: adding water into purified water with the temperature of 105-107 ℃ according to the mass ratio of 2:1, stirring to dissolve the water, sieving the mixture by using a 80-100-mesh sieve, and stirring to obtain primary slurry;

step S2: boiling the preliminary slurry in the step S1 at the temperature of 135-142 ℃ and concentrating to 3-5% of water content to obtain concentrated slurry;

step S3: cooling the concentrated slurry obtained in the step S2 to 60-80 ℃, adding citric acid and lysozyme into the concentrated slurry, uniformly stirring, standing for 2-3h, and keeping the temperature of 60-80 ℃ all the time during standing to obtain a finished product slurry;

step S4: and pouring the finished product slurry into a mold for molding, and demolding after cooling.

7. The preparation method of the anti-caries lollipop according to claim 6, wherein the preparation method of the glucosyl stevioside comprises the following steps:

step a: mixing stevioside and dextrin according to a mass ratio of 1: 0.5-3, and dissolving in water, wherein the mass concentration of the stevioside is 1-20%;

step b: b, adding cyclodextrin glucoside transferase into the solution obtained in the step a, wherein the addition amount of the cyclodextrin glucoside transferase is 1% -5% of the mass of stevioside, and uniformly stirring;

step c: c, reacting the solution obtained in the step b for 24-48h at the temperature of 60-70 ℃;

step d: after the reaction in the step c is finished, adding dextrin to continue the reaction for 5 hours, wherein the adding amount of the dextrin is 10% -20% of the paste amount in the step S1;

step e: heating the reactant in the step d to 90-105 ℃, and inactivating the enzyme;

step f: after cooling, filtering the reaction solution in the step e by using activated carbon to remove color and impurities;

step g: preparing the solution obtained in the step f into a solution with the mass concentration of 1% -5%, passing through macroporous adsorption resin until the solution is completely adsorbed, wherein the flow rate is 1BV/h, washing the adsorption column by using 2 times of the volume of the adsorption column, and washing away residual dextrin;

step h: eluting with 40% -50% ethanol solution 4 times the volume of the adsorption column, and eluting the unreacted stevioside and glucosyl stevioside mixture between macroporous adsorption resin resins at flow rate of 0.5 BV/h; collecting the effluent liquid with the volume of 1-1.5BV when the ultraviolet absorbance of the effluent liquid is more than 0.1-0.3;

step i: concentrating the effluent, and spray drying to obtain glucosyl stevioside.

Technical Field

The invention relates to the technical field of food processing, in particular to a decayed tooth preventing lollipop and a preparation method thereof.

Background

It is critical to health to have a good bite, but the world health organization's global database of oral health shows that 60% to 90% of pupils and 100% of adults are globally estimated to have decayed teeth, and untreated decayed teeth have become the most prevalent of 291 major diseases and injuries worldwide. Decayed teeth, also called as decayed teeth, is known as dental caries. The main cause of its formation is dental plaque. Dental plaque is an almost colorless film of the tooth surface containing caries-causing bacteria. After people eat, the bacteria in the dental plaque can react with sugar or starch in food to generate acidic substances which corrode teeth. Over time, the enamel of the teeth is destroyed, forming small caries, and if it continues to deteriorate, a cavity, i.e., a decayed tooth, is formed.

In the process of tooth decay, diet is an important function of bacteria. The food contains a large amount of carbohydrates and sugars which supply energy for the life and activity of bacteria in plaque, and also allow glycolysis by bacterial metabolism to produce organic acids which are retained for a long time on the tooth surface and in the pits, demineralizing and destroying enamel, followed by some bacteria and dissolving proteins to form cavities. The key to preventing dental caries is to reduce or control dietary sugar.

Traditional lollipops mainly comprise sweeteners such as white granulated sugar and maltose syrup, and after children eat the lollipops, the candies stay on the surfaces of teeth or gaps of the teeth, so that the possibility of dental caries is increased. At present, some patent documents for preventing dental caries exist in China, such as: 1. in the invention of patent application No. 200990113038.7, licorice lollipop for inhibiting dental caries formation, licorice extract is made into sugar block or lollipop which can be released in oral cavity slowly, and can significantly inhibit dental caries formation of the legal person of the subject. However, the liquorice cake is not discovered by the pharmaceutical industry to have the spectrum antibacterial capability, and the liquorice can be uncomfortable and sweet later, so that the lollipop made of the liquorice cake has poor taste; 2. the patent with the application number of 201510917965.1, namely 'a heat-clearing teeth-protecting lollipop', adopts traditional Chinese herbal medicines of liquorice, mint leaves, honeysuckle and the like as raw materials to manufacture the lollipop, has certain bacteriostatic and teeth-protecting effects, but the taste of the Chinese herbal medicine lollipop is estimated to be poor and is not favored by general people; 3. the invention relates to a method for preparing a candy for preventing dental caries and a lollipop thereof, which has application number of 201611268074.9, and the invention comprises a method for preparing soybean peptide concentrated crystal powder and adding soybean peptide diluent into a lollipop of a candy box. Although the soybean peptide is beneficial to human health and has a certain antibacterial effect, the soybean peptide is directly added into candies or lollipops, the sugar content on teeth is not reduced, only has a certain antibacterial effect, and the possibility of reducing decayed teeth is limited.

Therefore, a decayed tooth preventing lollipop suitable for children is needed.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a lollipop for preventing dental caries and a preparation method thereof, so as to prevent dental caries in children.

In a first aspect, the invention provides a lollipop for preventing dental caries, wherein glucosyl stevioside and stevioside are added into the lollipop for preventing dental caries as sweetening agents.

Optionally, the composition comprises the following components in parts by weight: 0.1-5 parts of glucosyl stevioside, 0.1-1 part of stevioside, 90-98 parts of isomalt, 0.005-0.05 part of lysozyme and 1-3 parts of citric acid.

Optionally, the glucosyl steviol glycoside is obtained by introducing glucosyl into steviol glycoside with cyclodextrin glucoside transferase.

Optionally, the glucosyl steviol glycoside has a purity of at least 80%.

Optionally, the rebaudioside a content of the steviol glycoside is greater than 90 wt%.

In a second aspect, the invention provides a preparation method of the anti-caries lollipop, which comprises the following steps:

step S1: accurately weighing glucosyl stevioside, stevioside and isomaltitol, and uniformly mixing, wherein the mass ratio of the components is as follows: adding water into purified water with the temperature of 105-107 ℃ according to the mass ratio of 2:1, stirring to dissolve the water, sieving the mixture by using a 80-100-mesh sieve, and stirring to obtain primary slurry;

step S2: boiling the preliminary slurry in the step S1 at the temperature of 135-142 ℃ and concentrating to 3-5% of water content to obtain concentrated slurry;

step S3: cooling the concentrated slurry obtained in the step S2 to 60-80 ℃, adding citric acid and lysozyme into the concentrated slurry, uniformly stirring, standing for 2-3h, and keeping the temperature of 60-80 ℃ all the time during standing to obtain a finished product slurry;

step S4: and pouring the finished product slurry into a mold for molding, and demolding after cooling.

Optionally, the preparation method of the glucosyl stevioside comprises the following steps:

step a: mixing stevioside and dextrin according to a mass ratio of 1: 0.5-3, and dissolving in water, wherein the mass concentration of the stevioside is 1-20%;

step b: b, adding cyclodextrin glucoside transferase into the solution obtained in the step a, wherein the addition amount of the cyclodextrin glucoside transferase is 1% -5% of the mass of stevioside, and uniformly stirring;

step c: c, reacting the solution obtained in the step b for 24-48h at the temperature of 60-70 ℃;

step d: after the reaction in the step c is finished, adding dextrin to continue the reaction for 5 hours, wherein the adding amount of the dextrin is 10% -20% of the paste amount in the step S1;

step e: heating the reactant in the step d to 90-105 ℃, and inactivating the enzyme;

step f: after cooling, filtering the reaction solution in the step e by using activated carbon to remove color and impurities;

step g: preparing the solution obtained in the step f into a solution with the mass concentration of 1% -5%, passing through macroporous adsorption resin until the solution is completely adsorbed, wherein the flow rate is 1BV/h, washing the adsorption column by using 2 times of the volume of the adsorption column, and washing away residual dextrin;

step h: eluting with 40% -50% ethanol solution 4 times the volume of the adsorption column, and eluting the unreacted stevioside and glucosyl stevioside mixture between macroporous adsorption resin resins at flow rate of 0.5 BV/h; collecting the effluent liquid with the volume of 1-1.5BV when the ultraviolet absorbance of the effluent liquid is more than 0.1-0.3;

step i: concentrating the effluent, and spray drying to obtain glucosyl stevioside.

The invention has the beneficial effects that:

1. the lollipop takes glucose-based stevioside and stevioside as main sweeteners, has natural and rich sweet taste, is not decomposed by oral bacteria in an oral cavity, does not cause decayed teeth, consolidates the teeth and strengthens the teeth; the isomaltitol is added, is rich in bifidobacterium proliferation factors, promotes the growth and the reproduction of the bifidobacterium, maintains the micro-ecological balance of intestinal tracts and is beneficial to the health of human bodies.

2. The lollipop is particularly added with lysozyme, can inhibit bacterial glycolysis and acid production, control the growth of oral cavity decayed teeth and has good effect of preventing the decayed teeth.

3. The lollipop prepared by the application is rich in taste, and can maintain intestinal microecological balance and prevent dental caries; the preparation process is simple and is suitable for industrial production.

4. One of raw materials for preparing the lollipop is glucosyl stevioside, a sweetening agent without after-bitterness is obtained by introducing glucosyl into stevioside through cyclodextrin glucoside transferase (CGtase), and the sweetening agent is pure and natural in sweet taste and has natural fruit fragrance.

Detailed Description

Hereinafter, embodiments of the present invention will be described in detail. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.

One of raw materials stevioside for preparing lollipop in the application is a natural sweetener extracted from stevia rebaudiana leaves by using a water extraction method, is a mixture of tetracyclic diterpenoid compounds with similar structures, has the characteristics of high sweetness and low calorie, and is praised as 'plant sugar king', 'best natural sweetener' and the like internationally. The anti-caries effect of stevioside is the best of all sweeteners, firstly because of the non-fermentation property of stevioside, stevioside cannot be utilized by bacteria in the oral cavity, nutrient substances required by the growth of the bacteria are reduced, and acid in the oral cavity is also reduced; and the stevioside has a promoting effect on saliva, so that the trend of pH reduction is slowed down, the teeth are prevented from being acidified and damaged, and the purpose of strengthening and strengthening the teeth is achieved.

One of the raw materials for preparing the lollipop is glucosyl stevioside, which is a sweetening agent without after-bitterness and obtained by introducing glucosyl into stevioside through cyclodextrin glucoside transferase (CGtase), wherein the sweetening agent is pure white in color, pure and natural in sweet feeling, has natural fruit fragrance, and has the sweetness which is about more than 200 times that of cane sugar.

The glucosyl stevioside is prepared by biological enzyme fermentation, and is prepared by grafting glucosyl molecule onto steviol C13 or C19, has better sweetness and taste than stevioside, and is not used by bacteria in oral cavity, so as not to cause dental caries. The glucosyl stevioside not only reduces the original bitter taste of stevioside, but also has the fragrance of natural fruits and has certain antioxidant activity. The glucose-based stevioside serving as a sweetening agent is added into the lollipop, so that the lollipop is healthy and safe, has rich mouthfeel, prevents decayed teeth, and enables people, particularly children, to eat the lollipop at ease.

Isomaltitol, which is one of raw materials for preparing lollipops, is called isomalt abroad, is a functional edible sugar alcohol which is newly developed internationally in recent years, and is an ideal sugar substitute. The isomaltitol is non-cariogenic, streptococcus mutans in oral cavity can not be decomposed and utilized, acid and glucan are not produced, and the isomaltitol is not cariogenic, is not capable of causing decayed teeth, and is particularly suitable for children. Isomalt is an excellent bifidobacterium proliferation factor, and although isomalt cannot be utilized by human bodies and enzyme systems of most microorganisms, isomalt can be decomposed and utilized by bifidobacteria in human intestinal tracts, so that growth and propagation of bifidobacteria are promoted, the microecological balance of the intestinal tracts is maintained, and the health of human bodies is facilitated.

The lysozyme, one of the raw materials for preparing the lollipop, is prepared by cloning and extracting through a bioengineering technology, is a natural enzyme, is a safe and green additive and has no drug resistance. Lysozyme is a very stable protein, can selectively decompose microbial cell walls without damaging other biological tissues, and has no irritation and no toxicity. The oral mutans streptococcus is the most main pathogenic bacteria of human caries, and the cariogenic property of the oral mutans streptococcus is mainly expressed in the aspects of adhesion to tooth surfaces, acid production, acid resistance, polysaccharide and bacteriocin production and the like. The lysozyme can inhibit the glycolysis and acid production of thalli and control the growth of oral decayed teeth, so the lysozyme has good effect of preventing the decayed teeth. The bacterial enzyme is widely applied to the field of oral disease treatment because of definite antibacterial effect, safety, no toxicity and difficult generation of drug-resistant strains.

The lollipop provided by the application is not recommended to be eaten by children under three years old due to the addition of isomalt. Because the audience of the lollipop is more than children, the mould of the lollipop can be made into various lovely shapes. The color of the lollipop can be enriched by adding natural pigment, so that the lollipop is more popular, the pigment can be added in a solution formula, and the color needs to be matched with the variety.

The first embodiment of the application provides a lollipop for preventing dental caries, wherein glucosyl stevioside and stevioside serving as sweetening agents are added into the lollipop for preventing dental caries, and the lollipop comprises the following components in parts by weight: 0.1-5 parts of glucosyl stevioside, 0.1-1 part of stevioside, 90-98 parts of isomalt, 0.005-0.05 part of lysozyme and 1-3 parts of citric acid.

The glucosyl stevioside is obtained by introducing glucosyl into stevioside by adopting cyclodextrin glucoside transferase. The purity of the glucosyl stevioside is at least 80%.

The rebaudioside A content in the stevioside is more than 90 wt%.

In a second embodiment, the present application provides a method for preparing a lollipop for preventing dental caries, comprising the steps of:

step S1: accurately weighing glucosyl stevioside, stevioside and isomaltitol, uniformly mixing, dissolving in purified water, and mixing according to the following solid amount: adding water into purified water with the temperature of 105-107 ℃ according to the mass ratio of 2:1, stirring to dissolve the water, standing for a moment after boiling, sieving the mixture by using a 80-100-mesh sieve to remove impurities, ensuring the transparency, and stirring to obtain primary slurry;

step S2: decocting the preliminary slurry in the step S1 at the temperature of 135-142 ℃ and concentrating to 3-5% of water content to obtain concentrated slurry, wherein the material liquid decocting time is not too long and is controlled within 3 min;

step S3: cooling the concentrated slurry obtained in the step S2 to 60-80 ℃, adding citric acid and lysozyme into the concentrated slurry, uniformly stirring, standing for 2-3h, and keeping the temperature of 60-80 ℃ all the time during standing to obtain a finished product slurry;

step S4: and pouring the finished product slurry into a mold for molding, and demolding after cooling. Upon cooling, the generation of bubbles should be repeatedly destroyed until the massecuite has good plasticity.

During operation, the material should be prevented from being lost on the table top, and the table top is prevented from being adhered.

A third embodiment of the present application provides a method for preparing glucosyl steviol glycoside, comprising the steps of:

step a: mixing stevioside and dextrin according to a mass ratio of 1: 0.5-3, and dissolving in water, wherein the mass concentration of the stevioside is 1-20%;

step b: b, adding cyclodextrin glucoside transferase into the solution obtained in the step a, wherein the addition amount of the cyclodextrin glucoside transferase is 1% -5% of the mass of stevioside, and uniformly stirring;

step c: c, reacting the solution obtained in the step b for 24-48h at the temperature of 60-70 ℃;

step d: after the reaction in the step c is finished, adding dextrin to continue the reaction for 5 hours, wherein the adding amount of the dextrin is 10% -20% of the paste amount in the step S1;

step e: heating the reactant in the step d to 90-105 ℃, and inactivating the enzyme;

step f: after cooling, filtering the reaction solution in the step e by using activated carbon to remove color and impurities;

step g: preparing the solution obtained in the step f into a solution with the mass concentration of 1% -5%, passing through macroporous adsorption resin until the solution is completely adsorbed, wherein the flow rate is 1BV/h, washing the adsorption column by using 2 times of the volume of the adsorption column, and washing away residual dextrin;

step h: eluting with 40% -50% ethanol solution 4 times the volume of the adsorption column, and eluting the unreacted stevioside and glucosyl stevioside mixture between macroporous adsorption resin resins at flow rate of 0.5 BV/h; collecting the effluent liquid with the volume of 1-1.5BV when the ultraviolet absorbance of the effluent liquid is more than 0.1-0.3;

step i: concentrating the effluent, and spray drying to obtain glucosyl stevioside.