阅读说明：本技术 一种止泻的中草药组合物及其制备方法 (Antidiarrheal Chinese herbal medicine composition and preparation method thereof ) 是由 简建设 于 2021-08-28 设计创作，主要内容包括：本发明提出了一种止泻的中草药组合物及其制备方法,涉及中药技术领域。一种止泻的中草药组合物,按重量份计,包括如下组分：五爪龙8-20份、白术4-12份和黄芪6-14份。此止泻的中草药组合物具有能够有效治疗动物腹泻的优点。(The invention provides a Chinese herbal medicine composition for relieving diarrhea and a preparation method thereof, relating to the technical field of traditional Chinese medicines. The Chinese herbal medicine composition for relieving diarrhea comprises the following components in parts by weight: 8-20 parts of radix seu folium Cayratiae Oligocarpae, 4-12 parts of rhizoma Atractylodis Macrocephalae and 6-14 parts of radix astragali. The antidiarrheal Chinese herbal medicine composition has the advantage of being capable of effectively treating animal diarrhea.)

1. The Chinese herbal medicine composition for relieving diarrhea is characterized by comprising the following components in parts by weight: 8-20 parts of radix seu folium Cayratiae Oligocarpae, 4-12 parts of rhizoma Atractylodis Macrocephalae and 6-14 parts of radix astragali.

2. The Chinese herbal medicine composition for relieving diarrhea according to claim 1, further comprising the following components in parts by weight: 10-14 parts of gentian, 10-14 parts of oldenlandia diffusa, 8-12 parts of hairyvein agrimony, 6-10 parts of phellodendron, 4-8 parts of motherwort, 4-8 parts of angelica, 4-8 parts of semen cuscutae and 4-8 parts of liquorice.

3. The Chinese herbal medicine composition for relieving diarrhea according to claim 1, further comprising the following components in parts by weight: 13-17 parts of divaricate saposhnikovia root, 11-15 parts of cimicifuga foetida, 10-14 parts of epimedium, 8-12 parts of rhizoma polygonati preparata and 8-12 parts of rhizoma atractylodis.

4. The Chinese herbal medicine composition for relieving diarrhea according to claim 1, further comprising the following components in parts by weight: 5-9 parts of honeysuckle, 6-10 parts of fructus forsythiae, 6-10 parts of oldenlandia diffusa, 5-9 parts of folium isatidis, 3-7 parts of gentian, 6-10 parts of scutellaria baicalensis, 3-7 parts of divaricate saposhnikovia root, 3-7 parts of rhizoma polygonati, 3-7 parts of radix bupleuri, 6-10 parts of hairyvein agrimonia herb and bud, 6-10 parts of red paeony root and 2-6 parts of liquorice.

5. The Chinese herbal medicine composition for relieving diarrhea according to claim 1, further comprising the following components in parts by weight: 6-10 parts of isatis root, 6-10 parts of common andrographis herb, 3-7 parts of radix bupleuri, 3-10 parts of cimicifuga foetida, 3-7 parts of divaricate saposhnikovia root, 6-10 parts of baical skullcap root, 4-8 parts of platycodon root, 3-7 parts of semen trichosanthis, 2-6 parts of pinellia ternate, 4-8 parts of epimedium herb, 4-8 parts of motherwort herb, 3-7 parts of south dodder seed and 1-5 parts of honey-fried licorice root.

6. The method for preparing an antidiarrheal herbal composition as claimed in any one of claims 1 to 5, comprising the steps of:

crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 30-90min, filtering, repeating for 1-4 times, and collecting filtrate;

mixing the filtrates, heating at 80-100 deg.C, concentrating to obtain colloidal filtrate, and collecting concentrated solution;

adding ethanol into the concentrated solution, stirring uniformly, standing for 40-56h, filtering, heating the filtrate at 80-110 ℃ to volatilize until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the weight ratio of the total volume of the liquid medicine to the raw medicinal materials to be (1-2): 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

7. The method for preparing an antidiarrheal herbal composition as claimed in claim 6, wherein the volume ratio of ethanol added to the concentrated solution is (2-2.5): 1, and the concentration of the ethanol is 95%.

8. The method for preparing an antidiarrheal herbal composition as claimed in any one of claims 1 to 5, comprising the steps of:

crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 60-120min, filtering, repeating for 2-4 times, and collecting filtrate;

centrifuging the filtrate, and filtering the supernatant with microporous membrane to obtain the final product.

9. The method as claimed in claim 8, wherein the centrifugation speed is 2500-3500r/min, the centrifugation time is 15-20 min; the filtration is carried out by passing through 0.35-0.45 μm microporous membrane under 0.065-0.08Mpa and 25-35 deg.C.

10. The method for preparing an antidiarrheal herbal composition as claimed in any one of claims 1 to 5, comprising the steps of: crushing the raw materials of each component to 300-500 meshes, and uniformly mixing to obtain the finished product of the micro powder of the traditional Chinese medicine composition.

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and particularly relates to a Chinese herbal medicine composition for relieving diarrhea and a preparation method thereof.

Background

The causes of diarrhea or dysentery in humans and animals have long been viral, bacterial, zoogenic, and dyspepsia due to poor feed management. So far, no medicine has good treatment effect on the diarrhea caused by the above causes in the clinical medicine and veterinarian.

Viral diarrhea: such as transmissible gastroenteritis of swine (TGE), Porcine Epidemic Diarrhea (PED), bovine viral diarrhea- -mucosal disease (BVD) and canine parvovirus disease. These are acute contact infections mainly caused by viruses and mainly caused by gastrointestinal damage, and are clinically characterized by high morbidity and rapid spread, and are mainly characterized by vomiting, diarrhea, gastrointestinal bleeding, necrotic and desquamation of gastrointestinal mucosa, loss or abolishment of appetite, dehydration and emaciation. The antibiotic has no pathogenic effect on treating viral diarrhea, and the existing chemical antiviral drugs have weak effects. Bacterial diarrhea: such as salmonellosis, colibacillosis, pasteurellosis of poultry, etc. which are common to humans and various animals. Diarrhea or dysentery caused by these bacteria is a common disease in farms, and although some antibiotics are effective, they are caused by: 1. the prevalence of bacterial presence increases the incidence of animal infections; 2. bacteria are easy to generate drug resistance; 3. the national requirement of antibiotics in the feed for organic animal food production (called "nonreactive" farming) is prohibitive, which over a period of time will make epidemic control in farming production more difficult. The aquaculture industry has called for the use of highly effective pure natural Chinese herbal medicines to control the occurrence of a variety of bacterial gastrointestinal diseases. Lientery: the diarrhea is caused by poor feeding management factors. At present, the medicines which can be independently used clinically by veterinarians and have outstanding curative effects are few, and the medicines are inconvenient to use in production. The occurrence of dysentery has the following common pathogenic characteristics: the first is the clinical manifestations of food or waste food reduction, some vomiting, diarrhea or watery diarrhea, some bloody stool or bloody dysentery, some mucosa abscission, dehydration, rapid emaciation and even death. Secondly, pathological changes are usually manifested as dehydration of appearance corpses, congestion and bleeding of gastrointestinal mucosa, and watery feces filled in intestines; the intestinal wall becomes thin and semitransparent, the mesentery is congested, and the mesentery lymph nodes are swollen to different degrees; villi of small intestinal mucosa become short and atrophy, and intestinal epithelium degeneration is obvious; the internal environment of the gastrointestinal tract is changed, the pH value is reduced, the microbial community is changed, and the secretion of digestive glands is obstructed.

The common method for treating diarrhea is mainly antimicrobial therapy (such as antiviral therapy, antibacterial therapy and antiparasitic therapy) with effective drugs.

At present, there is no effective treatment for diarrhea or dysentery caused by individual or combined diseases of the above-mentioned various etiological factors.

Disclosure of Invention

The invention aims to provide an antidiarrheal Chinese herbal medicine composition, which has the advantage of effectively treating animal diarrhea.

Another objective of the present invention is to provide a method for preparing three antidiarrheal herbal compositions, so as to obtain the Chinese herbal composition capable of effectively treating diarrhea in animals.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

On one hand, the embodiment of the application provides a Chinese herbal medicine composition for relieving diarrhea, which comprises the following components in parts by weight: 8-20 parts of radix seu folium Cayratiae Oligocarpae, 4-12 parts of rhizoma Atractylodis Macrocephalae and 6-14 parts of radix astragali.

In another aspect, the present application provides a method for preparing an antidiarrheal herbal composition, comprising the steps of:

crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 30-90min, filtering, repeating for 1-4 times, and collecting filtrate;

mixing the filtrates, heating at 80-100 deg.C, concentrating to obtain colloidal filtrate, and collecting concentrated solution;

adding ethanol into the concentrated solution, stirring uniformly, standing for 40-56h, filtering, heating the filtrate at 80-110 ℃ to volatilize until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the weight ratio of the total volume of the liquid medicine to the raw medicinal materials to be (1-2): 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

In another aspect, the present application provides a method for preparing an antidiarrheal herbal composition, comprising the steps of:

crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 60-120min, filtering, repeating for 2-4 times, and collecting filtrate;

centrifuging the filtrate, and filtering the supernatant with microporous membrane to obtain the final product.

In another aspect, the present application provides a method for preparing an antidiarrheal herbal composition, comprising the steps of:

crushing the raw materials of each component to 300-500 meshes, and uniformly mixing to obtain the finished product of the micro powder of the traditional Chinese medicine composition.

Five-claw dragon: herba Humuli Scandentis (also called herba Humuli Scandentis) is derived from herba Humulus scandens (Lour.) Merr. The morphological characteristics of the plants are as follows: the herbaceous plants are wound for many years. The stem has fine edges and small barbs. The leaf is paired, the palm of the leaf is 5-7 deep-cracked, has sawteeth and is 6-10cm long, and the hard bristles are scattered on two sides. The petiole has small hook thorn. In autumn, the flower is parthenocarpic, female and male, the male flower is a cone inflorescence, yellow green, the female inflorescence is short in spike shape, and the bract is light red green. The compound flower and fruit is approximately spherical. Collected in late autumn, and the whole herb is used for medicine. It is sweet, slightly bitter and cold in nature. Has the effects of clearing away heat and toxic materials, cooling blood, removing blood stasis, promoting urination, eliminating dampness, relieving diarrhea and dysentery, promoting digestion, dispelling pathogenic wind, removing dampness, removing blood stasis, and relieving swelling. Can be used for treating dyspepsia, rheumatism, flaccidity arthralgia, lumbago, leg pain, dysentery, diarrhea, enteritis, cough due to lung heat, edema, hyperpyrexia, skin sore, ulcer, leukorrhagia, lymphoid tuberculosis, traumatic injury, amenorrhea, and oliguria.

White atractylodes rhizome: bitter and sweet in taste and warm in nature. Has effects in invigorating qi, invigorating spleen, eliminating dampness, promoting diuresis, stopping sweating, and preventing miscarriage. Can be used for treating deficiency of spleen-qi, spleen deficiency, stagnation of spleen-qi, spleen deficiency, fluid retention, and excessive sweat due to muscle surface weakness and threatened abortion.

Astragalus root: sweet in flavor and slightly warm in nature. Has effects in invigorating spleen, strengthening middle warmer, invigorating yang, lifting sink, invigorating defensive qi, consolidating superficial resistance, promoting urination, removing toxic materials, and promoting granulation. It can be used for treating spleen qi deficiency, lung qi deficiency, spontaneous perspiration due to qi deficiency, deficiency of both qi and blood, and arthralgia syndrome.

Gentian: bitter taste and cold nature. Has the effects of clearing heat, drying dampness and purging liver and gallbladder fire. It is used for clearing away heat, eliminating dampness, purging pathogenic fire from liver and gallbladder, eliminating dampness, treating jaundice due to damp-heat, pudendal swelling, pudendal pruritus, leukorrhagia, eczema, pruritus, conjunctival congestion due to liver fire, tinnitus, deafness, hypochondriac pain, bitter taste in mouth, and convulsion.

Hedyotis diffusa: bitter and sweet in taste and cold in nature. Has the effects of clearing away heat and toxic material, promoting diuresis and treating stranguria. It can be used for treating carbuncle, swelling, sore throat, snake bite, stranguria with heat, and jaundice due to damp-heat pathogen.

Agrimony: bitter taste and cold nature. Has the effects of clearing heat, eliminating dampness, purging fire, removing toxic substances, and removing bone steaming. Can be used for treating leukorrhagia due to damp-heat, dysentery, tinea pedis due to damp-heat, hectic fever due to yin-deficiency, pyocutaneous disease, toxic swelling, eczema, and pruritus.

Motherwort: bitter and pungent in flavor and slightly cold in nature. Has effects of promoting blood circulation, regulating menstruation, inducing diuresis, relieving swelling, and clearing away heat and toxic materials. Can be used for treating menoxenia, dysmenorrhea, amenorrhea, lochiorrhea, edema, oliguria, pyocutaneous disease, and toxic swelling.

Chinese angelica: sweet and pungent in flavor and warm in nature. Has effects in tonifying blood, promoting blood circulation, regulating menstruation, relieving pain, and loosening bowel to relieve constipation. Can be used for treating blood deficiency, chlorosis, menoxenia, amenorrhea, dysmenorrhea, blood deficiency, blood stagnation, pain due to blood cold, traumatic injury, rheumatalgia, superficial infection, pyocutaneous disease, intestinal dryness, and constipation.

Dodder seed: sweet in flavor and warm in nature. Has the effects of tonifying yang and yin, securing essence and reducing urination, and improving eyesight and stopping diarrhea. Can be used for treating soreness of waist and knees, sexual impotence, spermatorrhea, frequent micturition, deficiency of liver and kidney, blurred vision, insufficiency of both the spleen and kidney, diarrhea, fetal irritability, and pregnant hemorrhage.

Licorice root: sweet in flavor and neutral in nature. Has the effects of invigorating qi, nourishing middle-jiao, moistening lung, relieving cough, clearing away heat and toxic materials, relieving spasm and pain, and regulating drug properties. Can be used for treating spleen deficiency, listlessness, palpitation, cough, asthma, carbuncle, cellulitis, pharyngitis, poisoning, abdominal distention, and spasm and pain of limbs.

Wind prevention: pungent and sweet in flavor, slightly warm in nature. Has the effects of dispelling pathogenic wind, relieving exterior syndrome, eliminating dampness, relieving pain and relieving spasm. Can be used for treating exterior syndrome due to exogenous pathogenic factor, rubella pruritus, rheumatalgia, and tetanus.

Cimicifugae foetidae: pungent, slightly sweet and slightly cold in nature. Has the effects of relieving exterior syndrome, promoting eruption, clearing away heat and toxic materials, and invigorating yang qi. Can be used for treating exterior syndrome due to exogenous pathogenic factors, measles without adequate eruption, toothache, aphtha, sore throat, warm toxic, macula, qi deficiency collapse, organ prolapse, metrorrhagia, and metrostaxis.

Herba epimedii: pungent and sweet in flavor and warm in nature. Has effects in invigorating kidney, supporting yang, strengthening muscle and bone, dispelling pathogenic wind, and removing dampness. It is mainly used for kidney yang deficiency, impotence, spermatorrhea, enuresis, frequent micturition, rheumatalgia, paralysis, etc.

Wine sealwort: sweet in flavor and neutral in nature. Has effects in invigorating qi, nourishing yin, invigorating spleen, moistening lung, and invigorating kidney. Can be used for treating weakness of spleen and stomach, asthenia, xerostomia, anorexia, lung deficiency, cough, essence and blood deficiency, and internal heat diabetes.

Rhizoma atractylodis: pungent and bitter with warm nature. Has the effects of eliminating dampness, strengthening spleen, dispelling pathogenic wind and dispelling cold. Can be used for treating syndrome of dampness obstruction in middle energizer, arthralgia due to wind-dampness, and exterior syndrome due to wind-cold-dampness.

Honeysuckle flower: sweet in flavor and cold in nature. Has the effects of clearing away heat and toxic materials and cooling and dispelling wind-heat. Can be used for treating carbuncle, furuncle, pharyngitis, erysipelas, toxic heat, dysentery, wind-heat type common cold, epidemic febrile disease, and fever.

Fructus forsythiae: bitter in taste and slightly cold in nature. Has the effects of clearing away heat and toxic materials, relieving swelling, dispersing pathogenic accumulation, and dispelling pathogenic wind and heat. It can be used for treating sore, carbuncle, toxic swelling, scrofula, subcutaneous nodule, wind-heat type common cold, early stage of epidemic febrile disease, and stranguria with damp-heat type.

Folium isatidis: bitter taste and cold nature. Has the effects of clearing away heat and toxic material, cooling blood and removing ecchymoses. Mainly treats heat entering nutrient blood, warm poison and macula, sore throat and aphtha and mumps erysipelas.

Scutellaria baicalensis: bitter taste and cold nature. Has effects in clearing away heat, eliminating dampness, purging pathogenic fire, removing toxic materials, stopping bleeding, and preventing miscarriage. Can be used for treating damp-warm syndrome, summer-heat dampness, chest distress, emesis, dampness-heat distention and fullness, jaundice, dysentery, cough due to lung heat, hyperpyrexia, polydipsia, hematemesis, carbuncle, swelling, sore, and threatened abortion.

Rhizoma polygonati: sweet in flavor and neutral in nature. Has effects in nourishing yin, moistening lung, invigorating spleen, invigorating qi, nourishing kidney, and replenishing essence. Can be used for treating yin deficiency, cough, dry cough, spleen deficiency, yellowish complexion, listlessness, anorexia, body fluid deficiency, dry mouth, red tongue, poor tongue fur, diabetes, kidney essence deficiency, soreness of waist and knees, sexual impotence, nocturnal emission, dizziness, tinnitus, dim eyesight, asthenia, emaciation, etc.

Bupleurum root: bitter and pungent in flavor and slightly cold in nature. Has the effects of relieving exterior syndrome, relieving fever, soothing liver, relieving depression and lifting yang qi. Can be used for treating fever due to exterior syndrome, shaoyang syndrome, stagnation of qi due to depression of liver-qi, qi deficiency, organ prolapse, and malaria due to fever.

Red peony root: bitter in taste and slightly cold in nature. Has the effects of clearing heat and cooling blood, and removing blood stasis and relieving pain. Can be used for treating macula due to toxic heat, hematemesis, epistaxis, conjunctival congestion, swelling and pain, liver depression, hypochondriac pain, amenorrhea, dysmenorrhea, abdominal pain, traumatic injury, carbuncle, swelling, and pyocutaneous disease.

Radix isatidis: bitter taste and cold nature. Has the effects of clearing away heat and toxic materials, cooling blood and relieving sore throat. Can be used for treating fever due to exogenous pathogens, early stage of epidemic febrile disease, sore throat, epidemic febrile disease, speckle, mumps, erysipelas, carbuncle, and suppurative sore.

Andrographis paniculata: bitter taste and cold nature. Has the effects of clearing away heat and toxic materials, cooling blood, relieving swelling, and eliminating dampness. Can be used for treating affection of exogenous wind-heat, early stage of epidemic febrile disease, cough and asthma due to lung heat, lung abscess, purulent vomiting, dysentery due to damp-heat, stranguria with heat, eczema, pruritus, carbuncle, suppurative sore, and snake and insect bite.

Balloon flower: bitter and pungent in flavor and mild in nature. Has effects in dispersing lung qi, eliminating phlegm, relieving sore throat, and expelling pus. Can be used for treating cough with excessive phlegm, chest distress, laryngopharynx swelling and pain, aphonia, lung abscess, and purulence.

Semen trichosanthis: sweet in flavor and cold in nature. Has the effects of moistening lung, eliminating phlegm, moistening intestine and relaxing bowels. Can be used for treating dry cough with sticky phlegm and constipation due to intestinal dryness.

Pinellia ternata: pungent flavor and warm nature. Has the effects of eliminating dampness and phlegm, lowering adverse qi and preventing vomiting, and relieving distension and fullness and dissipating nodulation. Can be used for treating damp phlegm, cold phlegm syndrome, emesis, epigastric distention and fullness, chest stagnation, globus hystericus, goiter, subcutaneous nodule, superficial infection, toxic snake bite.

Honey-fried licorice root: sweet in flavor and neutral in nature. Tonify spleen and qi, and harmonize the drugs. Can be used for treating swelling and pain of throat, cough, weakness of spleen and stomach, gastric and duodenal ulcer, hepatitis, carbuncle, furuncle, and toxic swelling, and drug and food poisoning.

The diarrhea of animals has various reasons, but all causes weakness of spleen and stomach and discomfort of intestines and stomach.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects: the invention has the effects of efficiently resisting virus and bacteria, clearing heat and detoxicating, tonifying middle-jiao and Qi, strengthening spleen and eliminating dampness, inducing diuresis and stopping diarrhea by synergistic action of the Wuzhua Longa, the astragalus and the bighead atractylodes rhizome, and can efficiently and stably cure the diarrhea and dysentery caused by various pathogens and various reasons. The invention has the double effects of antimicrobial therapy and supportive therapy at the same time, improves the cure rate of diseases, uses pure natural Chinese herbal medicine preparations, does not cause pathogenic microorganisms to generate drug resistance, and supports the sustainable development of animal husbandry production and the safe production of green organic animal food. The inventor creatively applies the traditional Chinese medicine therapy to animal dysentery, regulates the animal body from inside, and has the effects of tonifying middle-jiao and Qi, strengthening spleen, promoting urination and relieving diarrhea with astringents on animals. The invention removes non-alcohol-soluble substances (such as tannin) in filtrate by ethanol precipitation through water extraction and alcohol precipitation after decocting the raw materials, thereby extracting effective components in the liquid medicine and further harvesting the traditional Chinese medicine composition with stable quality and high clarity.

According to the invention, after the raw materials are decocted, the raw materials are filtered by the microporous filter membrane, so that impurities with large relative molecular mass in the filtrate can be effectively separated from substances with small relative molecular mass, the purity of the liquid medicine is improved, the viscosity of the product is reduced, the production time is shortened, the yield of the preparation is improved, the stability and the qualification rate of the product are improved, the precipitation of the product is reduced, and the quality of the prepared traditional Chinese medicine composition is good.

According to the invention, the raw materials are crushed to 300-500 meshes, so that the powder particles are fine, the cell walls of the raw materials are fully damaged, and then the raw materials are uniformly mixed, thereby improving the dissolution rate and the absorption rate of the Chinese herbal medicines.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

The invention provides a Chinese herbal medicine composition for relieving diarrhea, which comprises the following components in parts by weight: 8-20 parts of radix seu folium Cayratiae Oligocarpae, 4-12 parts of rhizoma Atractylodis Macrocephalae and 6-14 parts of radix astragali. Through the synergistic effect of the Wuzhua Longa, the radix astragali and the rhizoma atractylodis macrocephalae, the traditional Chinese medicine composition has the functions of efficiently resisting virus and bacteria, clearing away heat and toxic materials, tonifying middle-jiao and Qi, strengthening spleen and eliminating dampness, inducing diuresis and stopping diarrhea, and can efficiently and stably cure the dysentery caused by various pathogens and various reasons.

In some embodiments of the present invention, the traditional Chinese medicine composition further comprises the following components in parts by weight: 10-14 parts of gentian, 10-14 parts of oldenlandia diffusa, 8-12 parts of hairyvein agrimony, 6-10 parts of phellodendron, 4-8 parts of motherwort, 4-8 parts of angelica, 4-8 parts of semen cuscutae and 4-8 parts of liquorice. In the formula, the Wuzhua Longglong is used for clearing away heat and toxic materials, astringing to stop diarrhea, stopping bleeding and improving the disease resistance of the organism, and the modern medical research shows that: the herba Cayatiae Japonicae has antiviral, antibacterial, antiallergic, antiinflammatory, anti-stress, dysentery relieving, hemostatic, diuretic and immunoregulatory effects; radix Gentianae and herba Hedyotidis Diffusae have effects of clearing heat, eliminating dampness, cooling blood, removing toxic substances, resisting inflammation and promoting urination; the agrimony can astringe to stop bleeding and stop diarrhea, and is a preferred Chinese herbal medicine for treating intestinal bleeding and diarrhea; phellodendron bark, cortex Phellodendri can clear away damp-heat and purge fire-toxin, especially the heat in lower energizer, and has the protective action on blood platelets; radix astragali and parched Atractylodis rhizoma have effects of invigorating qi, invigorating yang, and invigorating spleen to consolidate constitution; angelica sinensis can enrich and activate blood; semen Cuscutae has effects in invigorating kidney, replenishing vital essence, and improving body resistance; licorice root, radix Glycyrrhizae has the effects of invigorating qi, strengthening the middle warmer, and alleviating the effects of herbs. The medicines combine together to achieve the effects of clearing away heat and toxic materials, stopping bleeding, stopping diarrhea, tonifying qi, consolidating the constitution, strengthening body resistance and eliminating pathogenic factors.

In some embodiments of the present invention, the traditional Chinese medicine composition further comprises the following components in parts by weight: 13-17 parts of divaricate saposhnikovia root, 11-15 parts of cimicifuga foetida, 10-14 parts of epimedium, 8-12 parts of rhizoma polygonati preparata and 8-12 parts of rhizoma atractylodis. The astragalus root in the formula has the effects of tonifying qi and the bighead atractylodes rhizome has the effects of tonifying spleen, preventing wind and guiding drug efficacy, specially achieving the aim of defense and exterior, tonifying lung, strengthening exterior and suppressing sweating, preventing cold, improving the immune globulin level and macrophage phagocytosis in blood of a body, and promoting the generation of interferon of the body, and is a representative formula capable of enhancing the nonspecific immunity function. On the basis, epimedium, polygonatum sibiricum wine and schisandra chinensis are added to warm the kidney, benefit yin and astringe the lung; rhizoma Atractylodis has effects in invigorating spleen and eliminating dampness; cimicifugae rhizoma can promote pathogenic toxin, and express exterior from interior, and promote healthy qi without sinking; the combination of the medicines can promote the functions of the Wuzhuanglong, such as clearing away heat and toxic material, stopping diarrhea and dysentery, resisting allergy, improving adaptability and enhancing the disease resistance of the organism to be played to the best. In addition, the formula can play a good role in soothing and treating animal stress response.

In some embodiments of the present invention, the composition further comprises the following components in parts by weight: 5-9 parts of honeysuckle, 6-10 parts of fructus forsythiae, 6-10 parts of oldenlandia diffusa, 5-9 parts of folium isatidis, 3-7 parts of gentian, 6-10 parts of scutellaria baicalensis, 3-7 parts of divaricate saposhnikovia root, 3-7 parts of rhizoma polygonati, 3-7 parts of radix bupleuri, 6-10 parts of hairyvein agrimonia herb and bud, 6-10 parts of red paeony root and 2-6 parts of liquorice. In the prescription, the five-claw dragon, the honeysuckle, the weeping forsythia, the spreading hedyotis herb, the indigowoad leaf and the baical skullcap root are used as main medicines for clearing away heat and toxic materials, resisting bacteria and viruses; radix astragali, rhizoma Atractylodis Macrocephalae, radix Saposhnikoviae, rhizoma Polygonati, invigorating qi, consolidating superficial resistance, strengthening vital qi, and eliminating pathogenic factors as adjuvants; radix Gentianae, herba et Gemma Agrimoniae, and radix Paeoniae Rubra with effects of clearing away liver-fire and gallbladder-heat, cooling blood, and stopping bleeding as adjuvant drugs; radix bupleuri and liquorice are used as the medicine for soothing the liver and regulating the flow of qi and moderating the medicine property. The medicines are combined to play the efficacies of stopping diarrhea and dysentery, clearing away heat and toxic material, tonifying qi and strengthening exterior, strengthening healthy qi and eliminating pathogenic factors, and cooling blood and stopping bleeding. So as to eliminate pathogenic toxin, strengthen the exterior, check healthy qi, dredge blood and vessels, stop bleeding and strengthen spleen yang. In addition, the formula can also prevent and treat subacute and acute African swine fever.

In some embodiments of the present invention, the composition further comprises the following components in parts by weight: 6-10 parts of isatis root, 6-10 parts of common andrographis herb, 3-7 parts of radix bupleuri, 3-10 parts of cimicifuga foetida, 3-7 parts of divaricate saposhnikovia root, 6-10 parts of baical skullcap root, 4-8 parts of platycodon root, 3-7 parts of semen trichosanthis, 2-6 parts of pinellia ternate, 4-8 parts of epimedium herb, 4-8 parts of motherwort herb, 3-7 parts of south dodder seed and 1-5 parts of honey-fried licorice root. In the embodiments of the invention, the Wuzhua Longa, the radix isatidis, the common andrographis herb and the baical skullcap root are used as main medicines for clearing away heat and toxic materials, resisting viruses, resisting bacteria and diminishing inflammation; radix astragali, parched Atractylodis rhizoma, radix Saposhnikoviae, herba Epimedii, semen Cuscutae, cimicifugae rhizoma, and adjuvants for invigorating qi, consolidating superficial resistance, warming kidney, nourishing yin, invigorating lung, and enhancing nonspecific immunity; radix platycodonis, pinellia ternate, semen trichosanthis and motherwort are used as adjuvant drugs for freeing lung and eliminating phlegm, clearing lung and eliminating phlegm, activating blood and removing stasis, and inducing diuresis to alleviate edema; radix bupleuri and liquorice sooth the liver and regulate qi and moderate the drug property as the harmonized drugs. The medicines are combined to achieve the effects of stopping diarrhea and dysentery, clearing heat and removing toxicity, tonifying qi and strengthening exterior, clearing lung and eliminating phlegm, and soothing liver and regulating qi, so that the body surface can be prevented from being invaded, the lung can be cleared and breathed smoothly, the liver can be soothed by blood, and the spleen can be healthy and healthy. The invention belongs to a pure natural Chinese herbal medicine 'no-resistance' prescription, and the safety of the product is not influenced after long-time application.

The invention also provides a preparation method of the antidiarrheal Chinese herbal medicine composition, which comprises the following steps: crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 30-90min, filtering, repeating for 1-4 times, and collecting filtrate; mixing the filtrates, heating at 80-100 deg.C, concentrating to obtain colloidal filtrate, and collecting concentrated solution; adding ethanol into the concentrated solution, stirring uniformly, standing for 40-56h, filtering, heating the filtrate at 80-110 ℃ to volatilize until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the weight ratio of the total volume of the liquid medicine to the raw medicinal materials to be (1-2): 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition. The invention removes non-alcohol-soluble substances (such as tannin) in filtrate by ethanol precipitation through water extraction and alcohol precipitation after decocting the raw materials, thereby extracting effective components in the liquid medicine and further harvesting the traditional Chinese medicine composition with stable quality and high clarity.

In some embodiments of the present invention, the volume ratio of the ethanol added to the concentrate is (2-2.5): 1, the concentration of ethanol is 95%.

The invention also provides a preparation method of the antidiarrheal Chinese herbal medicine composition, which comprises the following steps: crushing the raw materials of the components, adding the raw materials in a weight ratio of (8-10): 1, decocting for 60-120min, filtering, repeating for 2-4 times, and collecting filtrate; centrifuging the filtrate, and filtering the supernatant with microporous membrane to obtain the final product. According to the invention, after the raw materials are decocted, the raw materials are filtered by the microporous filter membrane, so that impurities with large relative molecular mass in the filtrate can be effectively separated from substances with small relative molecular mass, the purity of the liquid medicine is improved, the viscosity of the product is reduced, the production time is shortened, the yield of the preparation is improved, the stability and the qualification rate of the product are improved, the precipitation of the product is reduced, and the quality of the prepared traditional Chinese medicine composition is good.

In some embodiments of the present invention, the centrifugation speed is 2500-; the filtration is carried out by passing through 0.35-0.45 μm microporous membrane under 0.065-0.08Mpa and 25-35 deg.C.

The invention also provides a preparation method of the antidiarrheal Chinese herbal medicine composition, which comprises the following steps: crushing the raw materials of each component to 300-500 meshes, and uniformly mixing to obtain the finished product of the micro powder of the traditional Chinese medicine composition. According to the invention, the raw materials are crushed to 300-500 meshes, so that the powder particles are fine, the cell walls of the raw materials are fully damaged, and then the raw materials are uniformly mixed, thereby improving the dissolution rate and the absorption rate of the Chinese herbal medicines.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 8g of five-claw dragon, 4g of bighead atractylodes rhizome and 6g of astragalus root.

Crushing the raw materials, adding water which is 8 times of the weight of the raw materials, decocting for 30min, and filtering to obtain filtrate;

heating and concentrating the filtrate at 80 deg.C until the filtrate is gelatinous, and collecting concentrated solution;

adding ethanol with the concentration of 95% which is 2 times of the weight of the concentrated solution into the concentrated solution, uniformly stirring, standing for 40h, filtering, heating the filtrate at 80 ℃ to volatilize until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the weight ratio of the total volume of the liquid medicine to the raw medicinal materials to be 1: 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 2

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 14g of fiveleaf gynostemma herb, 8g of bighead atractylodes rhizome and 10g of astragalus root.

Crushing the raw materials, adding water which is 10 times of the weight of the raw materials, decocting for 60min, filtering, harvesting the filtrate, repeating the above operation on the filter residue, and filtering again to obtain the filtrate;

mixing the two filtrates, heating at 90 deg.C, concentrating to obtain colloidal filtrate, and collecting concentrated solution;

adding ethanol with the concentration of 95% and 2.3 times of the weight of the concentrated solution into the concentrated solution, stirring uniformly, standing for 48h, filtering, heating and volatilizing the filtrate at 90 ℃ until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the weight ratio of the total volume of the liquid medicine to the raw medicinal materials to be 1.5: 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 3

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 20g of fiveleaf gynostemma herb, 12g of bighead atractylodes rhizome and 14g of astragalus root.

Crushing the raw materials, adding water which is 12 times of the weight of the raw materials, decocting for 90min, filtering, harvesting the filtrate, and repeating the above operation on the filter residue for 4 times to obtain filtrate which is filtered for multiple times;

mixing the filtrates, heating at 100 deg.C, concentrating to obtain colloidal filtrate, and collecting concentrated solution;

adding ethanol with the concentration of 95% of 2.5 of the weight of the concentrated solution into the concentrated solution, stirring uniformly, standing for 56h, filtering, heating and volatilizing the filtrate at 100 ℃ until the filtrate has no ethanol smell, adding water for injection into the concentrated filtrate, and adjusting the ratio of the total volume of the liquid medicine to the weight of the raw medicinal materials to be 2: 1, uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 4

The difference from example 1 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 8g of five-claw dragon, 4g of bighead atractylodes rhizome and 6g of astragalus root;

crushing the raw materials, adding water which is 8 times of the weight of the raw materials, decocting for 30min, and filtering to obtain filtrate;

centrifuging the filtrate at 2500r/min for 15min, collecting supernatant, and filtering with 0.35 μm microporous membrane (ceramic membrane) at 25 deg.C under 0.065Mpa to obtain the final product.

Example 5

The difference from example 2 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 14g of five-claw dragon, 8g of bighead atractylodes rhizome and 10g of astragalus root;

crushing the raw materials, adding water which is 9 times of the weight of the raw materials, decocting for 90min, filtering, harvesting the filtrate, repeating the above operation on the filter residue, and filtering again to obtain the filtrate;

centrifuging the filtrate at 3000r/min for 18min, collecting supernatant, and filtering with 0.075Mpa and 30 deg.C microporous membrane (ceramic membrane) to obtain the final product.

Example 6

The difference from example 3 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 20g of five-claw dragon, 12g of bighead atractylodes rhizome and 14g of astragalus root;

crushing the raw materials, adding water which is 10 times of the weight of the raw materials, decocting for 120min, filtering, harvesting the filtrate, and repeating the above operation on the filter residue for 4 times to obtain filtrate which is filtered for multiple times;

centrifuging the filtrate at 3500r/min for 20min, collecting supernatant, and filtering with 0.45 μm microporous membrane at 35 deg.C under 0.08Mpa to obtain the final product.

Example 7

The difference from example 1 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 8g of five-claw dragon, 4g of bighead atractylodes rhizome and 6g of astragalus root;

crushing the raw materials of each component to 300 meshes, and uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 8

The difference from example 2 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 14g of five-claw dragon, 8g of bighead atractylodes rhizome and 10g of astragalus root;

crushing the raw materials of each component to 350 meshes, and uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 9

The difference from example 3 is that filtration was carried out using a microfiltration membrane.

A method for preparing an antidiarrheal Chinese herbal composition comprises the following steps:

raw materials: 20g of five-claw dragon, 12g of bighead atractylodes rhizome and 14g of astragalus root;

crushing the raw materials of each component to 400 meshes, and uniformly mixing to obtain a finished product of the traditional Chinese medicine composition.

Example 10

This example differs from example 1 in that: the raw materials comprise 8g of radix unguis, 4g of scorched white atractylodes rhizome, 6g of astragalus root, 10g of gentian, 10g of spreading hedyotis herb, 8g of hairyvein agrimony, 6g of phellodendron, 4g of motherwort herb, 4g of angelica, 4g of dodder and 4g of liquorice.

Example 11

This example differs from example 2 in that: the raw materials include 16g of radix unguis, 8g of scorched white atractylodes rhizome, 10g of astragalus root, 12g of gentian, 12g of spreading hedyotis herb, 10g of hairyvein agrimony, 8g of phellodendron, 6g of motherwort, 6g of angelica, 6g of dodder and 6g of liquorice.

Example 12

This example differs from example 3 in that: the raw materials comprise 20g of Wuzhugong, 12g of scorched white atractylodes rhizome, 14g of astragalus root, 14g of gentian, 14g of spreading hedyotis herb, 12g of hairyvein agrimony, 10g of phellodendron, 8g of motherwort, 8g of angelica, 8g of dodder and 8g of liquorice.

Example 13

This example differs from example 4 in that: the raw materials comprise 8g of radix unguis, 4g of scorched white atractylodes rhizome, 6g of astragalus root, 10g of gentian, 10g of spreading hedyotis herb, 8g of hairyvein agrimony, 6g of phellodendron, 4g of motherwort herb, 4g of angelica, 4g of dodder and 4g of liquorice.

Example 14

This example differs from example 5 in that: the raw materials include 16g of radix unguis, 8g of scorched white atractylodes rhizome, 10g of astragalus root, 12g of gentian, 12g of spreading hedyotis herb, 10g of hairyvein agrimony, 8g of phellodendron, 6g of motherwort, 6g of angelica, 6g of dodder and 6g of liquorice.

Example 15

This example differs from example 6 in that: the raw materials comprise 20g of Wuzhugong, 12g of scorched white atractylodes rhizome, 14g of astragalus root, 14g of gentian, 14g of spreading hedyotis herb, 12g of hairyvein agrimony, 10g of phellodendron, 8g of motherwort, 8g of angelica, 8g of dodder and 8g of liquorice.

Example 16

This example differs from example 7 in that: the raw materials comprise 8g of radix unguis, 4g of scorched white atractylodes rhizome, 6g of astragalus root, 10g of gentian, 10g of spreading hedyotis herb, 8g of hairyvein agrimony, 6g of phellodendron, 4g of motherwort herb, 4g of angelica, 4g of dodder and 4g of liquorice.

Example 17

This example differs from example 8 in that: the raw materials include 16g of radix unguis, 8g of scorched white atractylodes rhizome, 10g of astragalus root, 12g of gentian, 12g of spreading hedyotis herb, 10g of hairyvein agrimony, 8g of phellodendron, 6g of motherwort, 6g of angelica, 6g of dodder and 6g of liquorice.

Example 18

This example differs from example 9 in that: the raw materials comprise 20g of Wuzhugong, 12g of scorched white atractylodes rhizome, 14g of astragalus root, 14g of gentian, 14g of spreading hedyotis herb, 12g of hairyvein agrimony, 10g of phellodendron, 8g of motherwort, 8g of angelica, 8g of dodder and 8g of liquorice.

Example 19

This example differs from example 1 in that: the raw materials comprise 8g of radix unguis, 4g of rhizoma atractylodis macrocephalae, 6g of radix astragali, 13g of radix saposhnikoviae, 11g of rhizoma cimicifugae, 10g of herba epimedii, 8g of rhizoma polygonati preparata and 8g of rhizoma atractylodis.

Example 20

This example differs from example 2 in that: the raw materials comprise 18g of radix unguis, 10g of scorched rhizoma atractylodis macrocephalae, 12g of astragalus, 15g of divaricate saposhnikovia root, 13g of rhizoma cimicifugae, 12g of epimedium, 10g of rhizoma polygonati preparata and 10g of rhizoma atractylodis.

Example 21

This example differs from example 3 in that: the raw materials include 20g of radix unguis, 12g of scorched rhizoma atractylodis macrocephalae, 14g of astragalus, 17g of divaricate saposhnikovia root, 15g of rhizoma cimicifugae, 14g of epimedium, 12g of rhizoma polygonati preparata and 12g of rhizoma atractylodis.

Example 22

This example differs from example 4 in that: raw materials comprise 8g of radix unguis, 4g of scorched rhizoma atractylodis macrocephalae, 6g of astragalus, 13g of divaricate saposhnikovia root, 11g of rhizoma cimicifugae, 10g of epimedium, 8g of rhizoma polygonati preparata and 8g of rhizoma atractylodis.

Example 23

This example differs from example 5 in that: the raw materials comprise 18g of radix unguis, 10g of scorched rhizoma atractylodis macrocephalae, 12g of astragalus, 15g of divaricate saposhnikovia root, 13g of rhizoma cimicifugae, 12g of epimedium herb, 10g of rhizoma polygonati preparata and 10g of rhizoma atractylodis.

Example 24

This example differs from example 6 in that: the raw materials include 20g of radix unguis, 12g of scorched rhizoma atractylodis macrocephalae, 14g of astragalus, 17g of divaricate saposhnikovia root, 15g of rhizoma cimicifugae, 14g of epimedium, 12g of rhizoma polygonati preparata and 12g of rhizoma atractylodis. Charred rhizoma Atractylodis Macrocephalae

Example 25

This example differs from example 7 in that: raw materials comprise 8g of radix unguis, 4g of scorched rhizoma atractylodis macrocephalae, 6g of astragalus, 13g of divaricate saposhnikovia root, 11g of rhizoma cimicifugae, 10g of epimedium, 8g of rhizoma polygonati preparata and 8g of rhizoma atractylodis.

Example 26

This example differs from example 8 in that: the raw materials comprise 18g of radix unguis, 10g of scorched rhizoma atractylodis macrocephalae, 12g of astragalus, 15g of divaricate saposhnikovia root, 13g of rhizoma cimicifugae, 12g of epimedium, 10g of rhizoma polygonati preparata and 10g of rhizoma atractylodis.

Example 27

This example differs from example 9 in that: the raw materials include 20g of radix unguis, 12g of scorched rhizoma atractylodis macrocephalae, 14g of astragalus, 17g of divaricate saposhnikovia root, 15g of rhizoma cimicifugae, 14g of epimedium, 12g of rhizoma polygonati preparata and 12g of rhizoma atractylodis.

Example 28

This example differs from example 1 in that: the raw materials comprise 8g of cairo stephania root, 4g of largehead atractylodes rhizome, 6g of astragalus root, 5g of honeysuckle flower, 6g of weeping forsythia, 6g of spreading hedyotis herb, 5g of indigowoad leaf, 3g of Chinese gentian root, 6g of baical skullcap root, 3g of divaricate saposhnikovia root, 3g of manyflower solomonseal rhizome, 3g of Chinese thorowax root, 6g of hairyvein agrimony, 6g of red paeony root and 2g of liquoric root.

Example 29

This example differs from example 2 in that: the raw materials comprise 10g of cairo stephania root, 5g of largehead atractylodes rhizome, 7g of astragalus root, 7g of honeysuckle flower, 8g of weeping forsythia, 8g of spreading hedyotis herb, 7g of indigowoad leaf, 5g of Chinese gentian root, 8g of baical skullcap root, 5g of divaricate saposhnikovia root, 5g of manyflower solomonseal rhizome, 5g of Chinese thorowax root, 8g of hairyvein agrimony, 8g of red paeony root and 4g of liquoric root.

Example 30

This example differs from example 3 in that: the raw materials comprise 20g of cairo stephania root, 12g of largehead atractylodes rhizome, 14g of astragalus root, 9g of honeysuckle flower, 10g of weeping forsythia, 10g of spreading hedyotis herb, 9g of indigowoad leaf, 7g of Chinese gentian root, 10g of baical skullcap root, 7g of divaricate saposhnikovia root, 7g of manyflower solomonseal rhizome, 7g of Chinese thorowax root, 10g of hairyvein agrimony, 10g of red paeony root and 6g of liquoric root.

Example 31

This example differs from example 4 in that: the raw materials comprise 8g of cairo stephania root, 4g of largehead atractylodes rhizome, 6g of astragalus root, 5g of honeysuckle flower, 6g of weeping forsythia, 6g of spreading hedyotis herb, 5g of indigowoad leaf, 3g of Chinese gentian root, 6g of baical skullcap root, 3g of divaricate saposhnikovia root, 3g of manyflower solomonseal rhizome, 3g of Chinese thorowax root, 6g of hairyvein agrimony, 6g of red paeony root and 2g of liquoric root.

Example 32

This example differs from example 5 in that: the raw materials comprise 10g of cairo stephania root, 5g of largehead atractylodes rhizome, 7g of astragalus root, 7g of honeysuckle flower, 8g of weeping forsythia, 8g of spreading hedyotis herb, 7g of indigowoad leaf, 5g of Chinese gentian root, 8g of baical skullcap root, 5g of divaricate saposhnikovia root, 5g of manyflower solomonseal rhizome, 5g of Chinese thorowax root, 8g of hairyvein agrimony, 8g of red paeony root and 4g of liquoric root.

Example 33

This example differs from example 6 in that: the raw materials comprise 20g of cairo stephania root, 12g of largehead atractylodes rhizome, 14g of astragalus root, 9g of honeysuckle flower, 10g of weeping forsythia, 10g of spreading hedyotis herb, 9g of indigowoad leaf, 7g of Chinese gentian root, 10g of baical skullcap root, 7g of divaricate saposhnikovia root, 7g of manyflower solomonseal rhizome, 7g of Chinese thorowax root, 10g of hairyvein agrimony, 10g of red paeony root and 6g of liquoric root.

Example 34

This example differs from example 7 in that: the raw materials comprise 8g of cairo stephania root, 4g of largehead atractylodes rhizome, 6g of astragalus root, 5g of honeysuckle flower, 6g of weeping forsythia, 6g of spreading hedyotis herb, 5g of indigowoad leaf, 3g of Chinese gentian root, 6g of baical skullcap root, 3g of divaricate saposhnikovia root, 3g of manyflower solomonseal rhizome, 3g of Chinese thorowax root, 6g of hairyvein agrimony, 6g of red paeony root and 2g of liquoric root.

Example 35

This example differs from example 8 in that: the raw materials comprise 10g of cairo stephania root, 5g of largehead atractylodes rhizome, 7g of astragalus root, 7g of honeysuckle flower, 8g of weeping forsythia, 8g of spreading hedyotis herb, 7g of indigowoad leaf, 5g of Chinese gentian root, 8g of baical skullcap root, 5g of divaricate saposhnikovia root, 5g of manyflower solomonseal rhizome, 5g of Chinese thorowax root, 8g of hairyvein agrimony, 8g of red paeony root and 4g of liquoric root.

Example 36

This example differs from example 9 in that: the raw materials comprise 20g of cairo stephania root, 12g of largehead atractylodes rhizome, 14g of astragalus root, 9g of honeysuckle flower, 10g of weeping forsythia, 10g of spreading hedyotis herb, 9g of indigowoad leaf, 7g of Chinese gentian root, 10g of baical skullcap root, 7g of divaricate saposhnikovia root, 7g of manyflower solomonseal rhizome, 7g of Chinese thorowax root, 10g of hairyvein agrimony, 10g of red paeony root and 6g of liquoric root.

Example 37

This example differs from example 1 in that: the raw materials comprise 8g of Wuzhugong, 4g of white atractylodes rhizome, 6g of astragalus root, 6g of isatis root, 6g of common andrographis herb, 3g of Chinese thorowax root, 3g of largetrifolioliolioliolioliolioliolioliolioliolious bugbane rhizome, 3g of divaricate saposhnikovia root, 6g of baical skullcap root, 4g of platycodon root, 3g of snakegourd seed, 2g of pinellia tuber, 4g of epimedium herb, 4g of motherwort herb, 3g of south dodder seed and 1g of honey-fried licorice root.

Example 38

This example differs from example 2 in that: the raw materials comprise 14g of Wuzhugong, 5g of white atractylodes rhizome, 7g of astragalus, 8g of isatis root, 8g of common andrographis herb, 5g of Chinese thorowax root, 7g of largetrifoliolioliolioliolioliolious bugbane rhizome, 5g of divaricate saposhnikovia root, 8g of baical skullcap root, 6g of platycodon root, 5g of snakegourd seed, 4g of pinellia tuber, 6g of epimedium herb, 6g of motherwort herb, 5g of south dodder seed and 3g of honey-fried licorice root.

Example 39

This example differs from example 3 in that: the raw materials comprise 20g of rhizoma gastrodiae, 12g of bighead atractylodes rhizome, 14g of astragalus, 10g of isatis root, 10g of common andrographis herb, 7g of radix bupleuri, 10g of cimicifugae foetidae, 7g of divaricate saposhnikovia root, 10g of baical skullcap root, 8g of platycodon root, 7g of semen trichosanthis, 6g of pinellia tuber, 8g of epimedium herb, 8g of motherwort herb, 7g of south dodder seed and 5g of honey-fried licorice root.

Example 40

This example differs from example 4 in that: the raw materials comprise 8g of Wuzhugong, 4g of white atractylodes rhizome, 6g of astragalus root, 6g of isatis root, 6g of common andrographis herb, 3g of Chinese thorowax root, 3g of largetrifolioliolioliolioliolioliolioliolioliolious bugbane rhizome, 3g of divaricate saposhnikovia root, 6g of baical skullcap root, 4g of platycodon root, 3g of snakegourd seed, 2g of pinellia tuber, 4g of epimedium herb, 4g of motherwort herb, 3g of south dodder seed and 1g of honey-fried licorice root.

EXAMPLE 41

This example differs from example 5 in that: the raw materials comprise 14g of Wuzhugong, 5g of white atractylodes rhizome, 7g of astragalus, 8g of isatis root, 8g of common andrographis herb, 5g of Chinese thorowax root, 7g of largetrifoliolioliolioliolioliolious bugbane rhizome, 5g of divaricate saposhnikovia root, 8g of baical skullcap root, 6g of platycodon root, 5g of snakegourd seed, 4g of pinellia tuber, 6g of epimedium herb, 6g of motherwort herb, 5g of south dodder seed and 3g of honey-fried licorice root.

Example 42

This example differs from example 6 in that: the raw materials comprise 20g of rhizoma gastrodiae, 12g of bighead atractylodes rhizome, 14g of astragalus, 10g of isatis root, 10g of common andrographis herb, 7g of radix bupleuri, 10g of cimicifugae foetidae, 7g of divaricate saposhnikovia root, 10g of baical skullcap root, 8g of platycodon root, 7g of semen trichosanthis, 6g of pinellia tuber, 8g of epimedium herb, 8g of motherwort herb, 7g of south dodder seed and 5g of honey-fried licorice root.

Example 43

This example differs from example 7 in that: the raw materials comprise 8g of Wuzhugong, 4g of white atractylodes rhizome, 6g of astragalus root, 6g of isatis root, 6g of common andrographis herb, 3g of Chinese thorowax root, 3g of largetrifolioliolioliolioliolioliolioliolioliolious bugbane rhizome, 3g of divaricate saposhnikovia root, 6g of baical skullcap root, 4g of platycodon root, 3g of snakegourd seed, 2g of pinellia tuber, 4g of epimedium herb, 4g of motherwort herb, 3g of south dodder seed and 1g of honey-fried licorice root.

Example 44

This example differs from example 8 in that: the raw materials comprise 14g of Wuzhugong, 5g of white atractylodes rhizome, 7g of astragalus, 8g of isatis root, 8g of common andrographis herb, 5g of Chinese thorowax root, 7g of largetrifoliolioliolioliolioliolious bugbane rhizome, 5g of divaricate saposhnikovia root, 8g of baical skullcap root, 6g of platycodon root, 5g of snakegourd seed, 4g of pinellia tuber, 6g of epimedium herb, 6g of motherwort herb, 5g of south dodder seed and 3g of honey-fried licorice root.

Example 45

This example differs from example 9 in that: the raw materials comprise 20g of rhizoma gastrodiae, 12g of bighead atractylodes rhizome, 14g of astragalus, 10g of isatis root, 10g of common andrographis herb, 7g of radix bupleuri, 10g of cimicifugae foetidae, 7g of divaricate saposhnikovia root, 10g of baical skullcap root, 8g of platycodon root, 7g of semen trichosanthis, 6g of pinellia tuber, 8g of epimedium herb, 8g of motherwort herb, 7g of south dodder seed and 5g of honey-fried licorice root.

Examples of the experiments

Treatment of canine parvovirus disease

From 6 months in 2017 to 10 months in 2020, the authors rely on Yifeng county kernel and animal clinics to perform traditional Chinese medicine treatment on young dogs suffering from distemper and parvovirus diseases which come to visit, and most of the young dogs achieve satisfactory curative effects. In order to explore the best administration route of the Chinese medicinal composition prepared in the application for treating canine distemper and parvovirus diseases, 185 puppies meeting the following conditions were selected, comparative tests of oral administration and retention enema treatment of examples 1-18 were performed, 5 puppies per group, 90 dogs were sequentially subjected to oral administration tests of the Chinese medicinal composition prepared in the formulas of examples 1-18, retention enema treatment tests of 90 dogs were sequentially subjected to the Chinese medicinal composition prepared in the formulas of examples 1-18, the remaining 5 dogs were used as control groups, the control groups were treated by western medicine according to conventional methods of infusion for enhancing resistance, anti-virus and anti-inflammation (quintuplet serum, ribavirin, ceftriaxone sodium, gentamicin injection), antiemetic (meton, VB6) and hemostasis (hemostatic injection), a WBC descender was added with coenzyme A, and a slightly longer course of disease was treated with sodium bicarbonate injection, preventing acidosis. The preparation is taken once a day and maintained for about 7 days.

The test cases must meet the following conditions: 1. severe vomiting, watery diarrhea, dehydration; 2. blood component analysis White Blood Cells (WBCs) were significantly reduced; 3. the quick test card for parvovirus diseases detects positive.

The curative effect test standard is as follows: the effect is good: the puppies eat food at the mouth, the appetite recovers, vomiting and diarrhea stop, and the spirit recovers; the effect is general: the puppies eat food with poor appetite, stop vomiting and diarrhea and have poor spirit; no effect is achieved: puppies vomit violently, diarrhea, dehydration, food waste, mental weariness, or even death.

The oral administration is preferably carried out at a frequency of 30-40 ml/time and once a day.

The enema catheter is prepared by taking a plastic tube 55cm long for a small aerator with the outer diameter of 5mm as a main tube of the intestinal catheter, cutting a 60cm long infusion tube as a core tube of the intestinal catheter, cutting a 3 multiplied by 10(mm) notch on the tube wall 2-3cm away from the head end of the main tube, cleaning and disinfecting the main tube and the core tube respectively, and then sleeving the core tube into the main tube for later use.

The sausage filling method comprises the following steps: the liquid medicine is pre-warmed for standby, 50ml of bowel washing soap water is pre-warmed for standby, the enema catheter is disinfected and lubricated for standby- > let the forelimb of the dog land, the two hind limbs are lifted by the owner- > the perianal hair is sheared and cleaned, the anus is lubricated and then the bowel catheter enters the anus- > is inserted for about 10-15cm, a small amount of warm soap water is injected from the core tube and slowly drawn back and forth, at the moment, the catheter is easier to insert, when the catheter is continuously inserted to 30-40cm, the soap water is completely injected and slightly drawn back and forth, the maintenance is maintained for 3 minutes- > the two hind limbs are put down to naturally stand, the core tube is drawn out when the bowel catheter is drawn back for about 10cm, at the moment, the bowel washing liquid flows out along the tube, the spittoon is used for connection (the bowel washing liquid possibly with bloody urine), the bowel is washed once again by using 50ml of warm injection water according to the method, the bowel washing liquid is led out- > → the hind limbs are lifted to be high back to the front and the back, when the bowel catheter is re-fed to the depth of 30-40cm, sucking the standby liquid medicine by a plastic syringe, slowly injecting, keeping for 3 minutes, slowly drawing out the intestinal catheter, and gently putting down the two hind limbs.

Table 1 shows the case of the oral group; table 2 shows the cases in the retention enema group.

TABLE 1

According to the conditions in table 1, comparing groups 1-3 and groups 10-12, it can be seen that the effect of groups 10-12 is the best, which indicates that the effect of the combination of Wuzhilong, Baizhu, Huangqi, Dangecao, Oldenlandia diffusa, agrimony, Huangbai, Yi mu Cao, Dang Gui, Tu Si Zi and gan Cao is the best; comparing groups 10-12, it can be seen that group 11 is the best in effect, which indicates that the effect of the traditional Chinese medicine composition prepared by the microporous membrane filtration method is better for the diarrhea of animals caused by canine distemper; comparing the control group, it can be seen that the effect through groups 1-18 is significantly better than the effect of the control group; comparing group 11, group 14 and group 17, it can be seen that group 14 is the most effective, indicating that the effect of the agent prepared by the second of the three methods of preparation is the best for the animal.

TABLE 2

Analysis and comparison of Table 2, comparison of groups 1-3 and groups 10-12, show that groups 10-12 have the best effect, which indicates that the best effect of the combination of the dragon's claw, the white atractylodes rhizome, the astragalus root, the gentian, the spreading hedyotis herb, the hairyvein agrimony, the phellodendron bark, the motherwort herb, the angelica, the dodder and the liquorice; comparing groups 10-18, it can be seen that group 12, group 14 and group 15 work best; comparing the control group, it can be seen that the effects through groups 1-12 are significantly better than the effects of the control group.

Comparing table 1 and table 2, it can be seen that the therapeutic effect of retention enema group is better, this is because, the puppy when suffering from this disease, and sick dog vomits violently, through oral mode feeding back, and the puppy is difficult for swallowing, spits out the medicine easily, and deep retention enema can be more remain the medicine in the puppy internally, can have more medicines to be absorbed, consequently, can obtain better therapeutic effect. In the early stage of the disease, the sick dog vomits violently, and when the water drops can not enter, deep retention enema is better; in the later stage of the disease, the symptoms of the sick dog are stable, the sick dog does not vomit any more, and the oral administration is realized, so the operation is simple.

Then 25 puppies suffering from distemper and parvovirus diseases which come to be treated before are selected for Chinese and western medicine combined treatment, and the treatment is divided into 5 groups. Group 1 treatment with western medicine: according to the treatment procedures of enhancing resistance, resisting toxicity and diminishing inflammation (quintuplet serum, ribavirin, ceftriaxone sodium and gentamicin needle), stopping vomit (metoclopramide and VB6) and stopping bleeding (hemostatic and allergy needle) in transfusion, coenzyme A is added for patients with WBC (blood pressure lowering syndrome), and sodium bicarbonate injection is used for patients with a longer course of disease in a proper amount to prevent acidosis. The preparation is taken once a day and maintained for about 7 days. The traditional Chinese medicine treatment comprises the following steps: group 2 was performed by retention enema treatment using the Chinese medicinal composition prepared in example 14, group 3 was performed by retention enema treatment using the Chinese medicinal composition prepared in example 23, group 4 was performed by retention enema treatment using the Chinese medicinal composition prepared in example 32, and group 5 was performed by retention enema treatment using the Chinese medicinal composition prepared in example 41, with the treatment results shown in table 3.

TABLE 3

It is evident that the treatment effect was good in groups 2 to 5 compared to group 1, but the recovery time was 12 days on average for puppies of group 2, 14 days on average for puppies of groups 3 and 5, and 16 days on average for puppies of group 4, and it is evident that the effect was good in group 2 and more effective in recovery of puppies.

Typical cases are as follows:

in 12 pm of 9 months and 2017, raising the dog owner Lizhi, and sending 4-month-old female Thiaddi Niuniu to clinic for diagnosis. Animal complaints: the disease occurs in the morning, the spirit is depressed, the appetite is exhausted, paroxysmal vomiting occurs, a water sample mucus is spitted out, and a yellow water sample is diluted.

And (3) clinical examination: t: at 37.8 deg.C, mucosa pale, dyspnea and dehydration can be seen. Blood component analysis results: WBC: 2.8X 109/L (normal reference value [1 ]: 6.8-11.8X 109/L); RBC (red cell count): 3.59X 1012/L (normal reference value [2 ]: 6.7X 1012/L); PLT (platelet count): 1333X 109/L (normal reference value: 300-900X 109/L). The test paper card for detecting canine parvovirus diseases (provided by Shanghai Kuailing Biotech Co., Ltd.) has obvious and positive T line detection. By reference to the test results, in combination with clinical symptoms, a diagnosis of canine parvovirus disease can be made.

The treatment process comprises the following steps: in the afternoon of 9 months and 12 days, intravenous infusion was administered: 100ml of 0.9% sodium chloride, 2 ribavirin injections, 2 gentamicin injections and 1 styptic; 100ml of 10% glucose injection, 2 pieces of ATP injection, 1 piece of VC injection, 10mg of mechlorethamine hydrochloride injection and 1 piece of coenzyme A powder injection. Intramuscular injection: 1 quintuplet serum (5 ml); ② 0.15ml (after diluted by 10 times) of epinephrine injection is injected intramuscularly. According to the enema method, 30ml of the Chinese medicinal composition prepared in example 11 was used for deep retention enema (the liquid feces extracted at the time of the first enema was red-stained bloody stool).

9 months and 13 am, the body temperature was 38.5 ℃, and a small amount of blood and a pseudo-membrane remained adhered on the surface of the T-shaped film. And (3) treatment: intravenous infusion administration was performed on a 12 day 2 vial basis with drops: 30ml of water injection for injection and 3ml of sodium bicarbonate injection. Intramuscular injection: the quintuplet serum is changed to be once a day, and half intramuscular injection is performed once; epinephrine needle minus. The enema of the traditional Chinese medicine is changed into 40ml once a day (the liquid dung extracted by the second enema is yellow and has no visible blood).

Three morning diagnoses in 9 months and 14 days: main complaints: yesterday, no vomiting and no diarrhea after going home in the afternoon, and today, the call is loud and bright. And (4) checking: the body temperature was 39.0 ℃, the surface of the nose still had a small amount of adhesive, and moisture was retained at the nose. The treatment method is the same as 9 months and 13 days.

Afternoon four diagnostic visits of 9 months and 15 days: main complaints: the patient does not vomit or diarrhea, the spirit is improved, the patient feels loud and bright, the tail is shaken, moisture exists at the nose, and the patient eats a little porridge in the morning. And (4) checking: the temperature was 39.0 ℃ and a small amount of adhering matter remained on the surface of the sheet. Blood component analysis review: WBC: 7.9X 109/L; RBC: 5.79X 1012/L; PLT: 1063X 109/L. The review result shows that the main indexes of blood components are close to normal. The treatment method comprises the following steps: the dog owner requested no infusion, so: 1 branch of the chlorphenamine is injected into muscle; ② 1 branch of gentamicin injection is intramuscular injection; ③ injecting quintuplet serum into half-branch muscle; fourthly, the traditional Chinese medicine decoction is changed into oral administration, and 40ml of the traditional Chinese medicine decoction is taken on the spot without vomit under the help of a host. Taking 100ml of the Chinese medicinal decoction to home according to the requirement of a host, and administrating 30ml of the decoction once a day for each time.

Telephone access 24 days 9 months, dog owner says: the Niuniu has good recovery, the dog food is eaten in the first two days, and the excrement is normal and useless. "

Comprehensive analysis shows that the canine parvovirus disease is also called canine infectious hemorrhagic enteritis and clinically shows enteritis type and myocarditis type. The enteritis is more common and is characterized by severe vomiting, bloody water diarrhea, dehydration, obvious reduction of leucocytes and hemorrhagic necrotic enteritis in small intestine. Western treatments often employ symptomatic medication, supportive therapy, and injection of hyperimmune serum, however, commercial serum titers and specificities are marginal. Our treatment of this disease adheres to the following principle of the combination of Chinese and Western: stopping water and food, and supporting transfusion; relieving vomit, stopping diarrhea, stopping bleeding, resisting toxin, resisting bacteria and diminishing inflammation; reduce stress and enhance immune function. Because sick dogs have the characteristic of severe vomiting, drugs are easy to cause stress reaction to cause vomiting after oral administration, and even effective drugs cannot play a role. The deep retention enema method can effectively avoid vomiting of sick dogs caused by drug administration, and is filled with heated Chinese herbal medicine decoction on the basis of warm soap water intestinal lavage for detoxification, so that the medicine can be fully absorbed and utilized, heat is added to an organism, and the activity of cells and enzymes can be favorably recovered; but also stimulates the nerve excitation in the wall of the intestinal canal to increase the intestinal peristalsis; and by absorption, the functions of the liver and spleen are promoted, thus accelerating the healing of the disease.

(II) treatment of duck flavivirus

In the period from 2016 to 2018, the applicant of the patent holds a control effect test of duck tembusu virus diseases (also called duck flavivirus diseases) organized by a prevention and control center of animal epidemic diseases in Jiangxi province, and performs two-stage tests on livestock and poultry fine breeds of Xinxin county in Yichun city in Jiangxi province and Jian city in Xinyun city in Jiangxi province in sequence, wherein 600 ducks are tested. According to the design of the disease control center test, the present inventors used the prescription of example 12 as one of several test groups for the post-challenge treatment of duck flavivirus. The test shows that: when the formula of the embodiment 12 is added according to 3% of daily ration, when laying ducks with duck flavivirus diseases are treated, the good performances of 0 mortality rate, lowest egg drop rate, lighter ovarian diseases, fast egg recovery, and better duck groups in hair color, feather cleanness degree, mental state, vitality and diet than other control groups are obtained, and compared with other control groups, the curative effect is very obvious.

(III) treatment of yellow-white dysentery of piglets

Symptoms are: the water sample is thin feces, yellow or gray yellow, and contains curd flakes and small bubbles. Thirst, less milk intake, dehydration, emaciation, coma or exhaustion of sick pigs.

The inventor of the application adopts the traditional Chinese medicine composition prepared in the embodiment 8 to feed according to 3% of daily ration to treat 600 sick piglets (preventing the sick piglets from being fed according to 2% of the daily ration), and the cure rate is more than 96% after the sick piglets are continuously used for 3-5 days. This shows that the Chinese medicinal composition prepared by the application has excellent treatment effect on diarrhea caused by yellow-white dysentery.

(IV) treating dysentery of other livestock and poultry with various pathogens

Treating dysentery of livestock and poultry due to various reasons such as: the traditional Chinese medicine composition can obtain exact and obvious curative effects when being used for treating transmissible gastroenteritis of pigs, porcine epidemic diarrhea, rotavirus disease, pup dyspepsia, bovine viral diarrhea, ovine escherichia coli diarrhea, diarrhea caused by equine unknown reasons, canine viral enteritis, canine dyspepsia diarrhea, rabbit diarrhea, diarrhea caused by poultry escherichia coli and the like, and can be tested in hundreds of tests.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.