阅读说明：本技术 使用CRISPR/Cpf1系统的基因组编辑组合物及其用途 (Genome editing compositions using CRISPR/Cpf1 system and uses thereof ) 是由 金龙三 高正宪 李姃美 文秀彬 于 2018-11-21 设计创作，主要内容包括：本发明涉及使用CRISPR/Cpf1系统的基因组编辑组合物及其用途,并且更具体地,涉及基因组编辑组合物,其包含：包含能够与靶核苷酸序列杂交的指导序列和连接至所述指导序列的3’末端的尿苷重复序列的CRISPR RNA(crRNA)或其编码DNA；以及Cpf1蛋白或其编码DNA,使用所述组合物的基因组编辑方法、转基因实体的构建方法以及转基因实体。使用CRIPSPR/Cpf1系统,本发明可以在基因组编辑中提高插失效率并降低脱靶活性,并且因此可以容易地构建具有插入其中(敲入)或从其中缺失(敲除)的期望基因的转化细胞或者转基因动物或植物。(The present invention relates to genome editing compositions using the CRISPR/Cpf1 system and uses thereof, and more particularly, to genome editing compositions comprising: CRISPR RNA (crRNA) or a DNA encoding thereof comprising a guide sequence capable of hybridizing to a target nucleotide sequence and a uridine repeat sequence linked to the 3' end of the guide sequence; and Cpf1 protein or DNA encoding the same, methods of genome editing, methods of constructing transgenic entities, and transgenic entities using the compositions. Using the CRIPSPR/Cpf1 system, the present invention can increase insertion efficiency and reduce off-target activity in genome editing, and thus can easily construct a transformed cell or a transgenic animal or plant having a desired gene inserted therein (knock-in) or deleted therefrom (knock-out).)