阅读说明：本技术 一种高通量制备均匀单乳液滴的装置及方法 (Device and method for preparing uniform single emulsion drops in high flux ) 是由 司廷 杨超宇 乔然 于 2020-08-19 设计创作，主要内容包括：本发明公开了一种高通量制备均匀单乳液滴的装置及方法,装置包括基座、腔体、毛细结构和致动单元；基座上开设有分散相液体通道,且基座上还开设有与分散相液体通道导通的扰动接口,扰动接口封装,致动单元通过扰动接口输入扰动；毛细结构安装在基座的底端,且毛细结构的顶端与分散相液体通道的出口端导通；腔体密封安装在基座的底端,且毛细结构置于腔体内；腔体上开设有驱动相入口,驱动相入口用于输入驱动相液体到腔体内,驱动相液体和分散相液体互不相溶；腔体的底端开设有与毛细结构的出口端同心的排出孔。通过致动单元发出的扰动信号的频率,实现对射流破碎的控制,实现了高通量生产单乳液滴,且保证了单乳液滴的尺寸均匀。(The invention discloses a device and a method for preparing uniform single emulsion drops in a high-flux manner, wherein the device comprises a base, a cavity, a capillary structure and an actuating unit; the base is provided with a dispersed phase liquid channel, the base is also provided with a disturbance interface communicated with the dispersed phase liquid channel, the disturbance interface is packaged, and the actuating unit inputs disturbance through the disturbance interface; the capillary structure is arranged at the bottom end of the base, and the top end of the capillary structure is communicated with the outlet end of the dispersed phase liquid channel; the cavity is hermetically arranged at the bottom end of the base, and the capillary structure is arranged in the cavity; the cavity is provided with a driving phase inlet, the driving phase inlet is used for inputting driving phase liquid into the cavity, and the driving phase liquid and the dispersed phase liquid are not dissolved with each other; the bottom end of the cavity is provided with a discharge hole which is concentric with the outlet end of the capillary structure. The control of jet flow crushing is realized through the frequency of a disturbance signal sent by the actuating unit, the high-flux production of single emulsion drops is realized, and the uniformity of the sizes of the single emulsion drops is ensured.)

1. A device for preparing uniform single emulsion drops in high flux is characterized by comprising a base, a cavity, a capillary structure and an actuating unit;

the base is provided with a dispersed phase liquid channel, the inlet end of the dispersed phase liquid channel is used for inputting dispersed phase liquid, the base is further provided with a disturbance interface communicated with the dispersed phase liquid channel, the disturbance interface is packaged, and the actuating unit inputs disturbance through the packaged disturbance interface;

the outlet end of the dispersed phase liquid channel is arranged at the bottom end of the base, the capillary structure is hermetically arranged at the bottom end of the base, and the top end of the capillary structure is communicated with the outlet end of the dispersed phase liquid channel;

the cavity is hermetically arranged at the bottom end of the base, and the capillary structure is arranged in the cavity;

the cavity is provided with a driving phase inlet, the driving phase inlet is used for inputting driving phase liquid into the cavity, and the driving phase liquid and the dispersed phase liquid are not dissolved with each other;

the bottom end of the cavity is provided with a discharge hole, and the discharge hole is concentric with the outlet end of the capillary structure.

2. High throughput uniform single emulsion droplet preparation apparatus according to claim 1 wherein the diameter of said discharge orifice is 2 times the diameter of the single emulsion droplet to be prepared;

the inner diameter of the outlet end of the capillary structure is equivalent to the diameter of the discharge hole, and the distance from the outlet end of the capillary structure to the discharge hole is equivalent to the inner diameter of the outlet end of the capillary structure;

and/or

The diameter calculation formula of the single emulsion drop is D ═ (6Q/pi f)^1/3，

Q is the flow rate of the dispersed phase liquid,

f is the excitation frequency input by the actuation unit to the perturbation interface.

3. The high throughput uniform single emulsion droplet preparation device of claim 1 further comprising a base plate and a support column;

the supporting column is installed at the top end of the base, the substrate is installed at the top end of the supporting column, the actuating unit is installed on the substrate, and the actuating end of the actuating unit inputs disturbance to the disturbance interface.

4. High throughput uniform single emulsion droplet preparation apparatus according to claim 1, wherein said actuation unit comprises a signal generator, a power amplifier and an actuator;

the signal generator is in signal connection with the power amplifier, and the power amplifier is in signal connection with the actuator;

and the disturbance signal sent by the signal generator is amplified by the power amplifier and then output to the disturbance interface through the actuator.

5. High throughput uniform single emulsion droplet preparation apparatus according to claim 4 wherein said disturbance interface is encapsulated by a thin film.

6. The device for high throughput preparation of uniform single emulsion droplets of claim 1, further comprising a first syringe pump in fluid connection with the dispersed phase inlet for injecting the dispersed phase liquid into the dispersed phase inlet;

the device for preparing uniform single emulsion droplets at high flux also comprises a second injection pump which is in conductive connection with the driving phase inlet and is used for injecting the driving phase liquid into the driving phase inlet,

or

The device for preparing uniform single emulsion drops with high flux also comprises a power pump and a buffer,

the power pump is in conductive connection with the buffer, the buffer is in conductive connection with the driving phase inlet,

the power pump is used for conveying the driving phase liquid into the buffer, and the buffer is used for buffering the driving phase liquid and conveying the driving phase liquid into the cavity.

7. The device for high throughput preparation of uniform single emulsion droplets of claim 6, further comprising a reservoir;

the liquid storage tank is used for storing the driving phase liquid;

the power pump is a peristaltic pump, and the buffer is a buffer bottle.

8. High throughput preparation of uniform single emulsion droplets according to any one of claims 1 to 7, further comprising an image acquisition module,

the image acquisition module is used for acquiring an image signal of the single emulsion drop dripped from the discharge hole;

and/or

The device for preparing uniform single emulsion drops in high flux also comprises a drop collecting module,

the liquid drop collecting module is arranged at the bottom end of the base and used for collecting single emulsion drops dropping from the discharge hole.

9. A method for high throughput preparation of uniform single emulsion droplets comprising the steps of:

continuously inputting driving phase liquid and forming a stable flow field;

continuously inputting dispersed phase liquid, wherein the driving phase liquid and the dispersed phase liquid are not dissolved mutually, inputting disturbance to the dispersed phase liquid, and the driving phase liquid coats the dispersed phase liquid coming out from the capillary outlet to form single emulsion droplets.

10. The method for high throughput preparation of uniform single emulsion droplets of claim 9, wherein the diameter of the single emulsion droplet is calculated by the formula: d ═ 6Q/pi f)^1/3，

D is the diameter of the single emulsion droplet to be prepared,

q is the flow rate of the dispersed phase liquid,

f is the excitation frequency of the disturbance.

Technical Field

The invention relates to the technical field of single emulsion droplet preparation, in particular to a device and a method for preparing uniform single emulsion droplets in a high-flux manner.

Background

Single emulsion droplets of micron and submicron size are of great importance in various scientific and engineering applications. Over the past few decades, emerging microfluidic technologies have been developed to produce monodisperse droplets, such as microfluidic chips, glass microcapillaries, and flow focusing technologies.

In scientific research and practical application, the preparation of single emulsion droplets is carried out in a droplet mode, the flux is small, and the preparation efficiency is low. If the flux is increased, the preparation of single emulsion droplets is converted from a droplet mode to a jet mode, the size distribution of the droplets in the jet mode is widened, and the breaking length of the jet has certain randomness.

Therefore, how to realize high-throughput production of single emulsion droplets and ensure uniform size of the single emulsion droplets is a technical problem to be solved urgently by those skilled in the art.

Disclosure of Invention

In view of the above, the first objective of the present invention is to provide a device for preparing uniform single emulsion droplets at high throughput, which can achieve high-throughput production of single emulsion droplets and ensure uniform size of the single emulsion droplets.

A second object of the present invention is to provide a method for preparing uniform single emulsion droplets at high throughput.

In order to achieve the first object, the present invention provides the following solutions:

a device for preparing uniform single emulsion drops in high flux comprises a base, a cavity, a capillary structure and an actuating unit;

the base is provided with a dispersed phase liquid channel, the inlet end of the dispersed phase liquid channel is used for inputting dispersed phase liquid, the base is further provided with a disturbance interface communicated with the dispersed phase liquid channel, the disturbance interface is packaged, and the actuating unit inputs disturbance through the packaged disturbance interface;

the outlet end of the dispersed phase liquid channel is arranged at the bottom end of the base, the capillary structure is arranged at the bottom end of the base, and the top end of the capillary structure is communicated with the outlet end of the dispersed phase liquid channel;

the cavity is hermetically arranged at the bottom end of the base, and the capillary structure is arranged in the cavity;

the cavity is provided with a driving phase inlet, the driving phase inlet is used for inputting driving phase liquid into the cavity, and the driving phase liquid and the dispersed phase liquid are not dissolved with each other;

the bottom end of the cavity is provided with a discharge hole, and the discharge hole is concentric with the outlet end of the capillary structure.

In a particular embodiment, the diameter of the discharge orifice is 2 times the diameter of the single emulsion droplet to be prepared;

the inner diameter of the outlet end of the capillary structure is equivalent to the diameter of the discharge hole, and the distance from the outlet end of the capillary structure to the discharge hole is equivalent to the inner diameter of the outlet end of the capillary structure;

and/or

The diameter of the single emulsion droplet to be prepared is calculated by the formula D ═ (6Q/pi f)1/3,

q is the flow rate of the dispersed phase liquid,

f is the excitation frequency input by the actuation unit to the perturbation interface.

In another embodiment, the high throughput apparatus for preparing uniform single emulsion droplets further comprises a substrate and a support column;

the supporting column is installed at the top end of the base, the substrate is installed at the top end of the supporting column, the actuating unit is installed on the substrate, and the actuating end of the actuating unit inputs disturbance to the disturbance interface.

In another specific embodiment, the actuation unit comprises a signal generator, a power amplifier, and an actuator;

the signal generator is in signal connection with the power amplifier, and the power amplifier is in signal connection with the actuator;

and the disturbance signal sent by the signal generator is amplified by the power amplifier and then output to the disturbance interface through the actuator.

In another embodiment, the perturbation interface is encapsulated by a thin film.

In another specific embodiment, the high-throughput apparatus for preparing uniform single emulsion droplets further comprises a first syringe pump in fluid connection with the dispersed phase inlet for injecting the dispersed phase liquid into the dispersed phase inlet;

the device for preparing uniform single emulsion droplets at high flux also comprises a second injection pump which is in conductive connection with the driving phase inlet and is used for injecting the driving phase liquid into the driving phase inlet,

or

The device for preparing uniform single emulsion drops with high flux also comprises a power pump and a buffer,

the power pump is in conductive connection with the buffer, the buffer is in conductive connection with the driving phase inlet,

the power pump is used for conveying the driving phase liquid into the buffer, and the buffer is used for buffering the driving phase liquid and conveying the driving phase liquid into the cavity.

In another specific embodiment, the high throughput apparatus for preparing uniform single emulsion droplets further comprises a reservoir;

the liquid storage tank is used for storing the driving phase liquid;

the power pump is a peristaltic pump, and the buffer is a buffer bottle.

In another specific embodiment, the device for preparing uniform single emulsion droplets in high flux further comprises an image acquisition module,

the image acquisition module is used for acquiring an image signal of the single emulsion drop dripped from the discharge hole;

and/or

The device for preparing uniform single emulsion drops in high flux also comprises a drop collecting module,

the liquid drop collecting module is arranged at the bottom end of the base and used for collecting single emulsion drops dropping from the discharge hole.

The various embodiments according to the invention can be combined as desired, and the embodiments obtained after these combinations are also within the scope of the invention and are part of the specific embodiments of the invention.

Without being limited to any theory, it can be seen from the above disclosure that, in the device for preparing uniform single emulsion droplets at high flux disclosed by the invention, when in use, the driving phase liquid is continuously introduced into the cavity to form a stable driving phase flow field, then the dispersed phase liquid is continuously introduced into the inlet end of the dispersed phase liquid channel, the actuating unit inputs disturbance to the dispersed phase liquid in the dispersed phase liquid channel through the disturbance interface to realize control on jet flow crushing of the dispersed phase liquid, and the dispersed phase liquid is discharged from the outlet end of the capillary structure, wrapped by the driving phase liquid and discharged from the discharge hole to form single emulsion droplets. The control of jet flow crushing is realized through the frequency of a disturbance signal sent by the actuating unit, the high-flux production of single emulsion drops is realized, and the uniformity of the sizes of the single emulsion drops is ensured.

In order to achieve the second object, the invention provides the following technical solutions:

a method for high throughput preparation of uniform single emulsion droplets comprising the steps of:

continuously inputting driving phase liquid and forming a stable flow field;

continuously inputting dispersed phase liquid, wherein the driving phase liquid and the dispersed phase liquid are not dissolved mutually, inputting disturbance to the dispersed phase liquid, and the driving phase liquid coats the dispersed phase liquid coming out from the capillary outlet to form single emulsion droplets.

In one embodiment, the diameter of the single emulsion droplet is calculated by the formula,

d is the diameter of the single emulsion droplet to be prepared,

q is the flow rate of the dispersed phase liquid,

f is the excitation frequency of the disturbance signal.

The invention discloses a method for preparing uniform single emulsion drops in high flux, which realizes the control of the jet flow crushing of dispersed phase liquid by inputting disturbance signals to the dispersed phase liquid, and forms single emulsion drops by wrapping the driving phase liquid. By controlling the frequency of the disturbance signal, the control of jet flow crushing is realized, the high-flux production of single emulsion drops is realized, and the uniform size of the single emulsion drops is ensured.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a device for preparing uniform single emulsion droplets in high flux according to the present invention;

FIG. 2 is a partially enlarged schematic view of FIG. 1;

FIG. 3 is a schematic diagram of a disturbance interface according to the present invention;

FIG. 4 is a micrograph of a typical droplet provided by the present invention;

FIG. 5 is a graph of particle size versus frequency provided by the present invention;

FIG. 6 is a schematic diagram of the apparatus for high throughput preparation of uniform single emulsion droplets provided by the present invention;

fig. 7 is a schematic view of a connection structure of the power pump, the buffer and the liquid storage tank provided by the invention.

Wherein, in fig. 1-7:

the device comprises a base 1, a cavity 2, a capillary structure 3, an actuating unit 4, a disperse phase liquid channel 101, a disturbance interface 102, a driving phase inlet 201, a discharge hole 202, a substrate 5, a support column 6, a signal generator 401, a power amplifier 402, an actuator 403, a power pump 7, a buffer 8, a liquid storage tank 9, an image acquisition module 10, a camera 1001, a computer 1002, a liquid drop collection module 11, a sealing ring 12, a first injection pump 13, a second injection pump 14 and a strobe light 15.

Detailed Description

In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying fig. 1 to 7 and the detailed description thereof.

As shown in FIG. 1, the invention discloses a device for preparing uniform single emulsion droplets in a high-flux manner, wherein the device for preparing uniform single emulsion droplets in a high-flux manner comprises a base 1, a cavity 2, a capillary structure 3 and an actuating unit 4.

The base 1 is provided with a dispersed phase liquid channel 101, an inlet end of the dispersed phase liquid channel 101 is used for inputting dispersed phase liquid, the base 1 is further provided with a disturbance interface 102 communicated with the dispersed phase liquid channel 101, the disturbance interface 102 is packaged, specifically, the disturbance interface 102 is packaged through a film, so that disturbance can be input to the disturbance interface 102.

The disperse phase liquid channel 101 comprises a first channel and a second channel, the second channel is vertically arranged, the first channel is horizontally arranged or arranged at a certain inclination angle with the horizontal direction, the disturbance interface 102 is arranged at the upstream of the disperse phase liquid channel 101, specifically, the disturbance interface 102 is positioned at the conduction position of the first channel and the second channel, and the bottom end of the disturbance interface 102 is in a conical shape with a gradually reduced cross section, so that the disperse phase liquid conveyed by the first channel is buffered conveniently.

The actuation unit 4 inputs a disturbance through the encapsulated disturbance interface 102. The structure of the actuation unit 4 is not limited, and may be any structure capable of achieving output disturbance.

The exit end of disperse phase liquid channel 101 is arranged at the bottom end of base 1, capillary structure 3 is hermetically mounted at the bottom end of base 1, and the top end of capillary structure 3 is communicated with the exit end of disperse phase liquid channel 101. Specifically, the capillary structure 3 is a capillary tube, the capillary tube is in an inverted cone shape, and the size of the top end of the inner cavity of the capillary tube is larger than the diameter size of the outlet end of the dispersed phase liquid channel 101, that is, the inner cavity of the capillary tube surrounds the outlet end of the dispersed phase liquid channel 101.

The cavity 2 is hermetically arranged at the bottom end of the base 1, and the capillary structure 3 is arranged in the cavity 2. Specifically, the top wall of the cavity 2 is provided with a first limiting step and a second limiting step in sequence from outside to inside, and correspondingly, the bottom end of the base 1 is provided with a third limiting step matched with the first limiting step, a fourth limiting step matched with the second limiting step and a fifth limiting step in sequence from outside to inside. The top wall of the first limiting step and the third limiting step have a certain gap, and the side wall of the first limiting step and the side wall of the fourth limiting step are tightly attached to limit the cavity 2 and the base 1. The bottom end of the second limiting step is provided with a groove, a sealing ring 12 is arranged in the groove and seals the horizontal planes of the second limiting step and the fourth limiting step, and the side wall of the second limiting step is in sealing connection with the side wall of the fifth limiting step.

The cavity 2 is provided with a driving phase inlet 201, the driving phase inlet 201 is used for inputting a driving phase liquid into the cavity 2, and the driving phase liquid and a dispersed phase liquid are not dissolved with each other, for example, one of the driving phase liquid and the dispersed phase liquid is oil, and the other is water.

The bottom end of the cavity 2 is provided with a discharge hole 202, and the discharge hole 202 is concentric with the outlet end of the capillary structure 3, so that smooth discharge of single emulsion droplets is ensured.

When the device for preparing uniform single emulsion drops in high flux is used, driving phase liquid is continuously introduced into the cavity 2 to form a stable driving phase flow field, then dispersing phase liquid is continuously introduced into the inlet end of the dispersed phase liquid channel 101, a disturbance signal is input into the actuating unit 4 through the disturbance interface 102 to the dispersing phase liquid in the dispersed phase liquid channel 101, the control of jet flow crushing of the dispersing phase liquid is realized, the dispersing phase liquid is discharged from the outlet end of the capillary structure 3, wrapped by the driving phase liquid and discharged from the discharge hole 202, and single emulsion drops are formed. The preparation of different single emulsion drops is realized through the frequency of the disturbance signal sent by the actuating unit 4, and the adjustment and control of the single emulsion drops are realized. In addition, the breaking frequency of the jet flow is controlled by the actuating unit 4, so that the defect that the flux cannot be considered by depending on a drop mode in the existing microfluidics for preparing uniform liquid drops can be effectively overcome.

In some of the embodiments, the device for preparing uniform single emulsion droplets at high throughput further comprises a base plate 5 and a support column 6, the support column 6 is mounted on the top end of the base 1, the base plate 5 is mounted on the top end of the support column 6, the actuating unit 4 is mounted on the base plate 5, and the actuating end of the actuating unit 4 inputs the disturbance to the disturbance interface 102. Specifically, the support column 6 is welded to the base 1, and may be detachably attached to the base 1 by a screw or the like. The substrate 5 may be directly welded to the support column 6, or may be detachably attached to the support column 6 by screws or the like.

In some of these embodiments, the invention discloses that the diameter of the discharge opening 202 is 2 times the diameter of the single emulsion droplets to be prepared, the inner diameter Di of the outlet end of the capillary structure 3 corresponds to the diameter d of the discharge opening 202, and the distance H from the outlet end of the capillary structure 3 to the discharge opening 202 corresponds to the inner diameter Di of the outlet end of the capillary structure 3. Under the condition of meeting the above conditions, the prepared single emulsion is uniform in dripping. As shown in fig. 2. It should be noted that the equivalence here may be equal, slightly larger or slightly smaller.

Further, the invention discloses that the diameter of the single emulsion drop is calculated by the formula D ═ (6Q/pi f)1/3, where Q is the flow rate of the dispersed phase liquid and f is the excitation frequency input by the actuation unit 4 to the disturbance interface 102. That is, the control of the volume of the single emulsion drop is realized by controlling the flow rate of the dispersed phase liquid and the excitation frequency of the disturbance interface 102 input by the actuating unit 4.

In some of the embodiments, it is specifically disclosed that the actuation unit 4 comprises a signal generator 401, a power amplifier 402 and an actuator 403, the signal generator 401 is in signal connection with the power amplifier 402, and the power amplifier 402 is in signal connection with the actuator 403; the disturbance signal generated by the signal generator 401 is amplified by the power amplifier 402 and then output to the disturbance interface 102 through the actuator 403.

The perturbation signal output by the signal generator 401 may be a variety of different perturbation signals, such as a sine wave, a sawtooth wave, a square wave, and the like. The actuator may take many forms, for example, a piezoelectric ceramic, an electromagnetic oscillator, or the like, which may provide the vibration.

In some embodiments, the apparatus for preparing uniform single emulsion droplets at high flux further comprises a power pump 7 and a buffer 8, the power pump 7 is in communication with the buffer 8, the buffer 8 is in communication with the driving phase inlet 201, the power pump 7 is used for delivering the driving phase liquid into the buffer 8, and the buffer 8 is used for buffering the driving phase liquid and delivering the driving phase liquid into the cavity 2. The pulse flow of the power pump 7 is attenuated after passing through the buffer 8, and is not influenced by the flow rate of the driving phase liquid within a certain range in the single emulsion droplet particle size influence parameters. Specifically, the invention discloses that the power pump 7 is a peristaltic pump, and the buffer 8 is a buffer bottle. The power pump 7 is not limited to the peristaltic pump described above, and may be another type of pump. The buffer bottle is not limited to the bottle structure, but may be other structures.

Note that the input of the dispersed phase liquid and the drive phase liquid may be realized by using syringe pumps as they are, and as shown in fig. 6, the syringe pump for injecting the dispersed phase is referred to as a first syringe pump 13 and the syringe pump for injecting the drive phase is referred to as a second syringe pump 14.

Further, the device for high throughput preparation of uniform single emulsion droplets further comprises a reservoir 9, the reservoir 9 being used to drive a reservoir of phase liquid. Only the driving phase liquid needs to be added into the liquid storage tank 9 under the condition that a large amount of driving phase liquid is needed, and the method has important practical value when single emulsion liquid drops are continuously prepared in a large scale.

In some embodiments, the apparatus for preparing uniform single emulsion droplets at high throughput further comprises an image acquisition module 10, wherein the image acquisition module 10 is used for acquiring image signals of the single emulsion droplets dripped from the discharge hole 202.

Specifically, the image acquisition module 10 includes an image acquirer and a controller, the controller is in signal connection with the image acquirer, and the image acquirer is located below the cavity 2 and used for acquiring image information and transmitting the acquired information to the controller. The image acquisition device is a camera 1001, a video camera, or the like, and the controller is a computer 1002 or the like.

In order to collect the signal of the single emulsion drop, the invention discloses a device for preparing uniform single emulsion drops in high flux, which further comprises a strobe light 15, wherein the strobe light 15 is arranged below the cavity 2 and is positioned at one side of the single emulsion drop.

Further, the invention discloses a device for preparing uniform single emulsion droplets in high flux, which further comprises a droplet collection module 11, wherein the droplet collection module 11 is arranged at the bottom end of the base 1 and is used for collecting the single emulsion droplets discharged from the discharge hole 202. Specifically, the droplet collection module 11 includes a container, and a collection liquid is contained in the container, and the collection liquid is immiscible with the driving phase liquid.

The invention actively controls the initial disturbance (including frequency and amplitude) of the jet by means of the actuating unit 4, the fluid flowing out from upstream being dominated by the external disturbance, which can control the breaking frequency of the jet. The invention can accurately control the jet flow crushing process, can control the particle size of liquid drops and the length of the jet flow, and can be widely used by adjusting the frequency interval of disturbance application by adjusting the natural crushing frequency. The invention can also be applied to fluids of different properties (viscosity, interfacial tension), in particular to the situation of different fluids in various practical applications.

According to the device for preparing the uniform single emulsion drop with high flux, the actuating unit 4 is adopted to control the initial disturbance, so that the precise control of the jet flow crushing process can be realized, particularly the crushing frequency of the jet flow is controlled, the device is widely applied to various drop micro-fluidic technologies, the defect that the flux cannot be taken into account depending on the drop mode in the existing micro-fluidic technology for preparing the uniform drop can be effectively overcome, and the device is particularly suitable for the scene of preparing a large amount of micro-drops by using the jet flow mode.

Another aspect of the present invention provides a method for high throughput preparation of uniform single emulsion droplets, comprising the steps of:

step S1: continuously inputting driving phase liquid and forming a stable flow field.

Step S2: continuously inputting dispersed phase liquid, wherein the driving phase liquid and the dispersed phase liquid are not dissolved mutually, inputting disturbance to the dispersed phase liquid, and the driving phase liquid coats the dispersed phase liquid coming out from the capillary outlet to form single emulsion droplets.

Specifically, the initial disturbance includes a frequency and amplitude, and the dispersed phase fluid flowing from upstream is dominated by the applied disturbance, which can control the break up frequency of the jet.

Specifically, the initial disturbance may be an axial disturbance, i.e. the flow velocity of the jet is periodically modulated by the external coupling energy (mechanical, electric field, magnetic field, etc.), as shown in fig. 3, it may be a sinusoidal signal, a sawtooth wave signal, a rectangular wave signal, etc., preferably a sinusoidal modulation; it is also possible to combine multiple modulations, preferably with the same period, although other combinations of signals are possible.

The initial disturbance frequency is set to be optimal near the natural breaking frequency of the jet, and the natural breaking frequency can be changed by changing the structural parameters and the fluid propertiesThe rate, and thus the interval at which the disturbance frequency is applied, is adjusted such that, when external disturbances can dominate the jet break-up, the diameter of the droplets produced is controlled entirely by the dispersed phase flow Q and the excitation frequency f. The calculation formula is as follows: d ═ 6Q/pi f)^1/3D is the diameter of the single emulsion drop to be prepared, Q is the flow rate of the dispersed phase liquid, and f is the excitation frequency of the disturbance signal. A typical drop microscope is shown in figure 4. And the droplet size can be varied over a wide range by adjusting the drive phase flow and the frequency of the external disturbance, with constant flow parameters, as shown in figure 5.

The initial disturbance amplitude can vary over a wide range, too little amplitude leading to uncontrolled jet breakup, too much amplitude leading to a switch from jet mode to drop mode, but monodisperse drops can also be produced as desired.

The invention discloses a method for preparing uniform single emulsion drops in high flux, which realizes the control of the jet flow crushing of dispersed phase liquid by inputting disturbance signals to the dispersed phase liquid, and forms single emulsion drops by wrapping the driving phase liquid. The preparation of different single emulsion drops is realized by controlling the frequency of the disturbance signal, and the adjustment and control of the volume of the single emulsion drops are realized. In addition, the defect that flux cannot be considered by depending on a drop mode in order to prepare uniform liquid drops in the existing microfluidics can be effectively overcome by controlling the breaking frequency of jet flow.

The terms "first", "second", and the like in the present invention are used for descriptive distinction and have no other special meaning.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and inventive features disclosed herein.