阅读说明：本技术 通过单向双重探针引物延伸的靶标富集 (Target enrichment by one-way dual probe primer extension ) 是由 D.伯格斯 B.C.戈德温 A.洛夫乔伊 B.米勒 J.普拉策 J-A.E.彭克勒 于 2018-12-19 设计创作，主要内容包括：本发明提供了用于富集核酸文库中的至少一种靶标核酸的方法。第一寡核苷酸与具有第一和第二衔接子的核酸文库中的靶标核酸杂交。用第一聚合酶延伸杂交的第一寡核苷酸,由此产生包括所述靶标核酸和延伸的第一寡核苷酸的第一引物延伸复合物。捕获所述第一引物延伸复合物,其相对于所述核酸文库富集,且第二寡核苷酸与所述靶标核酸杂交。用第二聚合酶延伸杂交的第二寡核苷酸,由此产生包括所述靶标核酸和延伸的第二寡核苷酸的第二引物延伸复合物,且进一步从所述第一引物延伸复合物释放延伸的第一寡核苷酸。(The present invention provides methods for enriching at least one target nucleic acid in a nucleic acid library. The first oligonucleotide hybridizes to a target nucleic acid in a library of nucleic acids having first and second adaptors. Extending the hybridized first oligonucleotide with a first polymerase, thereby generating a first primer extension complex comprising the target nucleic acid and the extended first oligonucleotide. Capturing the first primer extension complex enriched relative to the nucleic acid library and a second oligonucleotide hybridized to the target nucleic acid. Extending the hybridized second oligonucleotide with a second polymerase, thereby generating a second primer extension complex comprising the target nucleic acid and the extended second oligonucleotide, and further releasing the extended first oligonucleotide from the first primer extension complex.)