Bioconversion agent for preparing sugar by straw fermentation and preparation method thereof

文档序号:1083446 发布日期:2020-10-20 浏览:22次 中文

阅读说明:本技术 一种秸秆发酵制糖的生物转化剂及其制备方法 (Bioconversion agent for preparing sugar by straw fermentation and preparation method thereof ) 是由 张超然 于 2019-04-09 设计创作,主要内容包括:本发明公开了生物转化技术领域,具体领域为一种秸秆发酵制糖的生物转化剂及其制备方法,以麸皮30~50份、苜蓿草粉30~50份、稻壳25~40份、玉米面25~40份、豆粕20~30份、屎肠球菌0.007~0.01份、植物甾醇0.007~0.01份、粪肠球菌0.007~0.01份、双歧杆菌0.007~0.01份、木霉菌0.007~0.01份为原材料,麸皮中含有较多粗纤维和氨基苯甲酸,氨基苯甲酸是细胞分裂所必需的物质,其有助于微生物繁殖,苜蓿草粉是由苜蓿草按一定的茎叶比例制成的草粉,苜蓿草粉中富含氨基酸、矿物质及微量元素,为微生物繁殖和生长提供必要的营养元素,稻壳、玉米面及豆粕与麸皮和苜蓿草粉配合使用,功能均为为微生物繁殖和生长提供良好的营养环境,以使发酵效果佳,发酵时间短。(The invention discloses the technical field of biotransformation, in particular to a bioconversion agent for preparing sugar by straw fermentation and a preparation method thereof, which takes 30-50 parts of bran, 30-50 parts of alfalfa meal, 25-40 parts of rice hulls, 25-40 parts of corn meal, 20-30 parts of bean pulp, 0.007-0.01 part of enterococcus faecium, 0.007-0.01 part of phytosterol, 0.007-0.01 part of enterococcus faecalis, 0.007-0.01 part of bifidobacteria and 0.007-0.01 part of trichoderma as raw materials, wherein the bran contains more crude fibers and aminobenzoic acid which is a substance necessary for cell division and is beneficial to microbial propagation, the alfalfa meal is grass meal prepared from alfalfa according to a certain stem leaf proportion, the alfalfa meal is rich in amino acid, mineral substances and trace elements and provides necessary nutrient elements for microbial propagation and growth, the rice hulls, the corn meal and the bean meal are matched with the bran and the alfalfa meal, and the function of providing a good nutritional environment for microbial propagation and growth, so that the fermentation effect is good and the fermentation time is short.)

1. A biological conversion agent for preparing sugar by straw fermentation and a preparation method thereof are characterized in that: the raw materials and corresponding parts by weight are as follows: 30-50 parts of bran, 30-50 parts of alfalfa meal, 25-40 parts of rice hulls, 25-40 parts of corn flour, 20-30 parts of soybean meal, 0.007-0.01 part of enterococcus faecium, 0.007-0.01 part of phytosterol, 0.007-0.01 part of enterococcus faecalis, 0.007-0.01 part of bifidobacterium and 0.007-0.01 part of trichoderma.

2. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: the raw materials and corresponding parts by weight are as follows: 35-45 parts of bran, 35-45 parts of alfalfa meal, 30-35 parts of rice hulls, 30-35 parts of corn flour, 22-28 parts of soybean meal, 0.008-0.009 parts of enterococcus faecium, 0.008-0.009 parts of phytosterol, 0.008-0.009 parts of enterococcus faecalis, 0.008-0.009 parts of bifidobacterium and 0.008-0.009 parts of trichoderma.

3. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: the raw materials and corresponding parts by weight are as follows: 40 parts of bran, 40 parts of alfalfa meal, 32 parts of rice hulls, 32 parts of corn flour, 25 parts of soybean meal, 0.0085 part of enterococcus faecium, 0.0085 part of phytosterol, 0.0085 part of enterococcus faecalis, 0.0085 part of bifidobacteria and 0.0085 part of trichoderma.

4. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: said enterococcus faecium, said phytosterol, said enterococcus faecalis, said bifidobacterium and said trichoderma are all in powder form.

5. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: the content of viable bacteria of the enterococcus faecium, the phytosterol, the enterococcus faecalis, the bifidobacterium and the trichoderma is 200-500 hundred million/g.

6. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: the preparation method comprises the following steps:

1) putting bran, alfalfa meal, rice hulls, corn flour and bean pulp into a grinder according to corresponding weight parts, mixing and grinding to enable the particle size of the ground mixture to be 40-60 meshes;

2) adding enterococcus faecium, phytosterol, enterococcus faecalis, bifidobacterium and trichoderma into the mixture prepared in the step 1), and uniformly mixing to prepare the bioconversion agent for preparing sugar by fermenting straws.

7. The bioconversion agent for sugar production by straw fermentation and the preparation method thereof as claimed in claim 1, wherein: the using method comprises the following steps: adding water into the straws to be fermented, wherein the ratio of the straws to the water is 1:2, and adding a bioconversion agent for preparing sugar by fermenting the straws into the straws at the temperature of 10-50 ℃, wherein the addition amount of the bioconversion agent is 2-4% of the total mass of the straws.

Technical Field

The invention relates to the technical field of biotransformation, in particular to a bioconversion agent for preparing sugar by straw fermentation and a preparation method thereof.

Background

The straws are a general term of stem and leaf parts of mature crops, generally refer to the residual parts of wheat, rice, corn, potatoes, rape, cotton, sugarcane and other crops after harvesting seeds, more than half of products of crop photosynthesis exist in the straws, the straws are rich in nutrient elements such as nitrogen, phosphorus, potassium, calcium, magnesium and the like, wherein the content of crude fibers can reach 30-40%, the nutritional value of the straws can be greatly improved after fermentation, the fermented straws contain crude fibers which are converted into carbohydrates, the straws are easier to absorb after being eaten by animals, 5.8 tons of crop straws are produced in China each year, the straws account for 20-30% of the total yield of the straws in the world, the straws are considered to be one of the most abundant renewable resources in the world, how to fully utilize the straws has great significance on relieving global energy crisis, food and feed resources and environmental pollution, the existing bioconversion agent for producing sugar by straw fermentation has a great requirement on the quality of straws in the process of fermenting with the straws, if the straws are stored for a long time, fibers in the bioconversion agent lose water and nutrients are lost, and if the bioconversion agent is fermented under the condition, microorganisms in the bioconversion agent for producing sugar by straw fermentation are not easy to reproduce, so that the fermentation effect is poor and the fermentation time is long.

Disclosure of Invention

The invention aims to provide a bioconversion agent for producing sugar by straw fermentation and a preparation method thereof, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a bioconversion agent for preparing sugar by straw fermentation and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: 30-50 parts of bran, 30-50 parts of alfalfa meal, 25-40 parts of rice hulls, 25-40 parts of corn flour, 20-30 parts of soybean meal, 0.007-0.01 part of enterococcus faecium, 0.007-0.01 part of phytosterol, 0.007-0.01 part of enterococcus faecalis, 0.007-0.01 part of bifidobacterium and 0.007-0.01 part of trichoderma.

Preferably, the bioconversion agent for preparing sugar by straw fermentation and the preparation method thereof comprise the following raw materials in parts by weight: 35-45 parts of bran, 35-45 parts of alfalfa meal, 30-35 parts of rice hulls, 30-35 parts of corn flour, 22-28 parts of soybean meal, 0.008-0.009 parts of enterococcus faecium, 0.008-0.009 parts of phytosterol, 0.008-0.009 parts of enterococcus faecalis, 0.008-0.009 parts of bifidobacterium and 0.008-0.009 parts of trichoderma.

Preferably, the bioconversion agent for preparing sugar by straw fermentation and the preparation method thereof comprise the following raw materials in parts by weight: 40 parts of bran, 40 parts of alfalfa meal, 32 parts of rice hulls, 32 parts of corn flour, 25 parts of soybean meal, 0.0085 part of enterococcus faecium, 0.0085 part of phytosterol, 0.0085 part of enterococcus faecalis, 0.0085 part of bifidobacteria and 0.0085 part of trichoderma.

Preferably, said enterococcus faecium, said phytosterol, said enterococcus faecalis, said bifidobacterium and said trichoderma are all in powder form.

Preferably, the viable bacteria content of the enterococcus faecium, the phytosterol, the enterococcus faecalis, the bifidobacterium and the trichoderma is 200-500 hundred million/g.

Preferably, the biological transforming agent for preparing sugar by straw fermentation and the preparation method thereof comprise the following steps:

1) putting bran, alfalfa meal, rice hulls, corn flour and bean pulp into a grinder according to corresponding weight parts, mixing and grinding to enable the particle size of the ground mixture to be 40-60 meshes;

2) adding enterococcus faecium, phytosterol, enterococcus faecalis, bifidobacterium and trichoderma into the mixture prepared in the step 1), and uniformly mixing to prepare the bioconversion agent for preparing sugar by fermenting straws.

Preferably, the application method of the bioconversion agent for preparing sugar by straw fermentation comprises the following steps: adding water into the straws to be fermented, wherein the ratio of the straws to the water is 1:2, and adding a bioconversion agent for preparing sugar by fermenting the straws into the straws at the temperature of 10-50 ℃, wherein the addition amount of the bioconversion agent is 2-4% of the total mass of the straws.

The invention has the beneficial effects that: a straw ferments the bioconversion agent of sugar manufacturing and its preparation method, the wheat bran is the outermost epidermis of wheat, the wheat becomes flour and two parts of wheat bran after the flour-milling machine processing, there are more crude fiber and aminobenzoic acid in the wheat bran, aminobenzoic acid is the necessary substance of cell division, it helps the microorganism to reproduce, the alfalfa meal is the grass meal made according to certain stem leaf proportion by alfalfa, the alfalfa meal is rich in amino acid, mineral substance and microelement, provide the necessary nutrient element for the microorganism to reproduce and grow, rice husk, corn flour and soya bean pulp cooperate with wheat bran and alfalfa meal to use, the function is to provide the good nutrient environment for microorganism to reproduce and grow, so as to make the fermentation effect good, the fermentation time is short; enterococcus faecium, phytosterol, enterococcus faecalis, bifidobacterium and trichoderma all are microorganisms for decomposing cellulose in the straws, so that crude fibers contained in the straws are converted into carbohydrates which can be absorbed by animals more easily.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

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