阅读说明：本技术 一种用于蛋鸡的发酵饲用天然植物微生态饲料添加剂及其制备方法 (Natural plant micro-ecological feed additive for fermented feed of laying hens and preparation method thereof ) 是由 李克克 谢璐璐 朱广跃 韩亚坤 李光华 冯利菲 买超梁 于 2020-05-06 设计创作，主要内容包括：本发明涉及一种用于蛋鸡的发酵饲用天然植物微生态饲料添加剂及其制备方法,属于饲料添加剂领域。本发明的用于蛋鸡的发酵饲用天然植物微生态饲料添加剂的制备方法,包括以下步骤：将微生物、培养料和中药组合物的混合物在密闭条件下发酵,干燥即得；所述中药组合物包括甘草、黄芪和刺五加；所述微生物包括枯草芽孢杆菌、地衣芽孢杆菌、酿酒酵母菌、丁酸梭菌、嗜乳酸杆菌、粪肠球菌和干酪乳杆菌。本发明的用于蛋鸡的发酵饲用天然植物微生态饲料添加剂的制备方法制得的添加剂中,各活菌及其代谢产物共同作用,提高蛋鸡的免疫力及抗病能力,大大的降低了死淘率,并且无毒副作用、无药物残留,还能提高蛋鸡产蛋率、改善鸡蛋品质。(The invention relates to a natural plant micro-ecological feed additive for fermented feed of laying hens and a preparation method thereof, belonging to the field of feed additives. The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product; the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax; the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei. In the additive prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens, all the live bacteria and metabolites thereof act together to improve the immunity and disease resistance of the laying hens, greatly reduce the death and culling rate, have no toxic or side effect and no drug residue, and also can improve the laying rate of the laying hens and improve the quality of eggs.)

1. A preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens is characterized by comprising the following steps: the method comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product;

the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax;

the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum and lactic acid bacteria.

2. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the mass ratio of the liquorice, the astragalus and the acanthopanax is 25-30: 20-24: 10-15.

3. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1 or 2, wherein: the lactic acid bacteria include Lactobacillus acidophilus, enterococcus faecalis and Lactobacillus casei.

4. The preparation method of the natural plant micro-ecological feed additive for the fermented feed of laying hens according to claim 3The method is characterized in that the ratio of the total number of bacterial colonies of bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei is (2.5-4.2) × 10¹¹:(2.5～4.2)×10¹¹:(0.06～1.2)×10¹¹:(0.08～500)×10⁸:(0.4～8)×10¹¹:(0.4～4)×10¹¹:(0.4～8)×10¹¹。

5. The method for preparing a natural plant micro-ecological feed additive for a fermented feed of laying hens according to claim 1 or 2, wherein the total number of bacterial colonies of the bacillus subtilis adopted for every 25-30 g of liquorice is (2.5-4.2) × 10¹¹CFU。

6. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the culture material is selected from one or any combination of bran, corn, rice chaff and gunite corn husk.

7. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the mass ratio of the culture material to the liquorice is 36-40: 25-30.

8. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the fermentation temperature is 33-37 ℃, and the fermentation time is 5-7 days.

9. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1 or 8, wherein the natural plant micro-ecological feed additive comprises: the drying temperature is 50-70 ℃.

10. A natural plant micro-ecological feed additive for fermented feed for laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for fermented feed for laying hens according to claim 1.

Technical Field

The invention relates to a natural plant micro-ecological feed additive for fermented feed of laying hens and a preparation method thereof, belonging to the field of feed additives.

Background

The breeding of the laying hens has a plurality of problems at present, such as low and unstable laying rate, thin eggshells, light yolk color, unsuitable temperature and humidity of the environment or diseases, high death and culling rate caused by stress and the like, so that the breeding cost of the laying hens is increased invisibly. Blindly increasing the laying rate by increasing the feeding amount not only increases the cost, but also leads the chicken to be fat and fatty liver to appear too early, thus leading the death and culling rate to be increased; the disease control of the laying hens is not easy for culturists, and after the laying hens are started, when the laying hens have certain diseases and the medicines are necessary, some common medicines such as aminophylline, aureomycin, sulfonamides and the like can inhibit or damage the system of the laying hens.

Disclosure of Invention

The invention aims to provide a preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens, and the prepared additive can improve the laying rate of the laying hens, reduce the death and culling rate of the laying hens and has no toxic or side effect.

The invention also provides a natural plant micro-ecological feed additive for the fermented feed of the laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens.

In order to realize the aim, the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens adopts the technical scheme that:

a preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product; the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax; the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum and lactic acid bacteria.

The invention relates to a preparation method of a natural plant microecological feed additive for a fermented feed of laying hens, which takes a culture material and a traditional Chinese medicine composition as fermentation substrates, wherein the culture material provides nutrient substances required by the growth of microorganisms, the traditional Chinese medicine composition and the microorganisms have synergistic effect, the active ingredients of liquorice, astragalus and acanthopanax are improved by microbial transformation while providing cellulose carbon sources for the microorganisms, and polysaccharides, vitamins, amino acids and the like in the traditional Chinese medicine composition promote the growth and the propagation of clostridium butyricum, lactic acid bacteria, bacillus subtilis and bacillus licheniformis in the biotransformation process, and promote the generation of metabolic products such as cellulase, protease, amylase, butyric acid, lactic acid and the like. Meanwhile, the liquorice can effectively remove excessive free radicals generated by heat stress, enhance the antioxidation function of animal organisms, improve the feed intake and improve the stress state of animals; the astragalus has the function of immunoregulation, and improves the immunity and the stress state of animal organisms; the acanthopanax can stimulate the cell immunity and enhance the disease resistance of animals; the combination of the three components can enhance the immunity of animals, enhance the disease resistance, improve the feed intake and improve the stress state. Specific microorganisms are selected for closed fermentation, and the prepared additive contains active bacillus subtilis, bacillus licheniformis, clostridium butyricum, lactic acid bacteria, a small amount of saccharomyces cerevisiae, and also contains metabolic products such as amylase, protease, cellulase, lipase, lactic acid, butyric acid, amino acid, vitamin B, vitamin K, small peptide and the like generated by the additive; meanwhile, the traditional Chinese medicine is converted into small molecular components which can be directly absorbed by intestinal tracts of laying hens through microorganisms, so that the traditional Chinese medicine is easier to play a role. The clostridium butyricum resists the colonization of pathogenic bacteria through the same complex carbohydrate receptor on lipid and protein which compete with the pathogenic bacteria for the intestinal epithelium villus length, thereby improving the health level of the intestinal tract; the bacillus subtilis and the bacillus licheniformis can generate enzymes such as amylase, protease, cellulase, lipase and the like, improve the digestion capacity of small intestines, promote the digestion and absorption of food, and improve the utilization rate of feed, thereby improving the feed-egg ratio; meanwhile, bacillus subtilis and bacillus licheniformis can quickly consume oxygen to inhibit harmful bacteria such as escherichia coli and the like; amino acid, vitamin B, vitamin K, small peptide and the like generated by clostridium butyricum can provide richer and balanced nutrition for laying hens, and the generated butyric acid and lactic acid can reduce the pH value of intestinal tracts of the laying hens, inhibit the growth and reproduction of harmful bacteria such as escherichia coli and the like, are beneficial to the health of the intestinal tracts and reduce intestinal diseases such as diarrhea; the saccharomyces cerevisiae thalli can provide richer protein nutrition for the laying hens. The living bacteria and the metabolites thereof act together to improve the immunity and disease resistance of the laying hens, greatly reduce the death and culling rate, have no toxic or side effect and no drug residue, and can also improve the laying rate of the laying hens and the quality of eggs.

Preferably, the mass ratio of the liquorice, the astragalus and the acanthopanax is 25-30: 20-24: 10-15.

Preferably, the ratio of the total number of the bacterial colonies of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the clostridium butyricum and the lactic acid bacteria is (2.5-4.2) × 10¹¹:(2.5～4.2)×10¹¹:(0.06～1.2)×10¹¹:(0.08～500)×10⁸:(1.2～20)×10¹¹More preferably, the ratio of the total number of colonies of Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Clostridium butyricum, Lactobacillus acidophilus, enterococcus faecalis and Lactobacillus casei is (2.5-4.2) × 10¹¹:(2.5～4.2)×10¹¹:(0.06～1.2)×10¹¹:(0.08～500)×10⁸:(0.4～8)×10¹¹:(0.4～4)×10¹¹:(0.4～8)×10¹¹It should be noted that the total number of bacterial colonies in the present invention is obtained by multiplying the effective viable count (CFU/mL or CFU/g) of the bacterial strain by the amount (volume or mass) of the bacterial strain, and the total number of bacterial colonies of the Bacillus subtilis used per 25-30 g of licorice is (2.5-4.2) × 10¹¹CFU。

For example, the mixture is prepared using a process comprising the steps of: adding bacillus subtilis powder, bacillus licheniformis powder and saccharomyces cerevisiae powder into the mixed powder of the culture material, the liquorice, the astragalus and the acanthopanax, uniformly mixing, then adding the mixed bacterial liquid of clostridium butyricum and lactic acid bacteria, and uniformly mixing to obtain the compound fertilizer.

The compost, the liquorice, the astragalus and the acanthopanax mixed powder can be obtained by naturally airing and then crushing the compost, the liquorice, the astragalus and the acanthopanax, drying at 70-90 ℃ and then uniformly mixing.

In the preparation method of the mixture, the viable count of the bacillus subtilis powder is (1.0-1.2) × 10¹¹CFU/g, the viable count of the bacillus licheniformis bacterial powder is (1.0-1.2) × 10¹¹CFU/g, the viable count of the saccharomyces cerevisiae bacterial powder is (0.2-2.4) × 10¹¹CFU/g。

In the preparation method of the mixture, the mixed bacterial liquid is mixed bacterial liquid of clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei, and the effective viable count of the clostridium butyricum in the mixed bacterial liquid is (0.001-5.0) × 10⁸CFU/mL, effective viable count of Lactobacillus acidophilus of 0.5-8.0 × 10⁹CFU/mL, the effective viable count of enterococcus faecalis is (0.5-4.0) × 10⁹CFU/mL, effective viable count of Lactobacillus casei is (0.5-8.0) × 10⁹CFU/mL。

In the preparation method of the mixture, 80-100 mL of mixed bacterial liquid is adopted for every 25-30 g of liquorice.

In addition, the mixtures can also be prepared by the methods of method a, method B, method C or method D. The method A comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, Saccharomyces cerevisiae powder, Clostridium butyricum powder and lactobacillus powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding water, and stirring. The method B comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, Saccharomyces cerevisiae powder and lactobacillus powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding Clostridium butyricum liquid, and mixing. Method C comprises the steps of: adding bacillus subtilis powder, bacillus licheniformis powder, saccharomyces cerevisiae powder and clostridium butyricum powder into the mixed powder of the culture material, the liquorice, the astragalus and the acanthopanax, uniformly mixing, then adding lactobacillus liquid, and uniformly mixing to obtain the compound fertilizer, wherein the method D comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, and Saccharomyces cerevisiae powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding Clostridium butyricum liquid and lactobacillus liquid, and mixing.

Preferably, in the method A, 80-100 mL of water is adopted for every 25-30 g of liquorice. In the method B, 80-100 mL of clostridium butyricum bacterial liquid is correspondingly adopted for every 25-30 g of liquorice. In the method C, 80-100 mL of lactobacillus bacterial liquid is correspondingly adopted for every 25-30 g of liquorice. In the method D, 80-100 mL of clostridium butyricum bacterial liquid and 0.5-2 mL of lactobacillus bacterial liquid are correspondingly adopted for every 25-30 g of liquorice.

Preferably, the culture material is selected from one or any combination of bran, corn, rice chaff and gunite corn chaff.

Preferably, the mass ratio of the culture material to the liquorice is 36-40: 25-30.

Preferably, the fermentation temperature is 33-37 ℃ and the fermentation time is 5-7 days.

Preferably, the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens further comprises drying the product obtained after fermentation. Further preferably, the temperature of the drying treatment is 50 to 70 ℃.

The product obtained by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens has the effective viable count that the bacillus subtilis is more than or equal to 1.0 × 10⁹CFU/g, more than or equal to 1.0 × 10 of bacillus licheniformis⁹CFU/g, Saccharomyces cerevisiae ≥ 1.0 × 10²CFU/g, Clostridium butyricum is more than or equal to 1.0 × 10⁷CFU/g, lactic acid bacteria not less than 1.0 × 10⁹CFU/g。

The invention relates to a natural plant micro-ecological feed additive for fermentation feed of laying hens, which adopts the technical scheme that:

the natural plant micro-ecological feed additive for the fermented feed of the laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens.

The natural plant micro-ecological feed additive for the fermented feed of the laying hens has no toxic or side effect and no drug residue, can improve the laying rate of the laying hens, improve the egg quality and reduce the death rate of the laying hens, and the effective viable count of the natural plant micro-ecological feed additive for the fermented feed of the laying hens can reach that the number of bacillus subtilis is more than or equal to 1.0 × 10⁹CFU/g, more than or equal to 1.0 × 10 of bacillus licheniformis⁹CFU/g, Saccharomyces cerevisiae ≥ 1.0 × 10²CFU/g, Clostridium butyricum is more than or equal to 1.0 × 10⁸CFU/g, lactic acid bacteria not less than 1.0 × 10⁹CFU/g. Beneficial microorganisms in the additive can regulate intestinal microecological balance, enhance immunity of laying hens, inhibit growth and reproduction of harmful microorganisms, and reduce risk and probability of diseases of chicken flocks, such as short-chain fatty acids such as butyric acid and lactic acid produced by clostridium butyricum and lactic acid bacteria, can stimulate intestinal peristalsis and improve intestinal microenvironment; the hydrolase such as cellulase, amylase, protease and the like generated by the bacillus licheniformis and the bacillus subtilis can promote the digestion and absorption of nutrient substances; more importantly, the functions of the probiotics can not generate toxic and harmful substances and side effects, can not generate drug residues, accords with advocated non-resistance and resistance reduction, provides health protection and navigation for animals and plants, and provides green and nutritional food for human beings.

Detailed Description

The present invention will be further described with reference to the following embodiments.