阅读说明：本技术 一种具有改善畜禽肠道功能的菌-酶融合发酵培养物及其制备方法 (Bacterium-enzyme fusion fermentation culture with livestock and poultry intestinal function improving function and preparation method thereof ) 是由 赵怀宝 陈亮 张宏福 于 2020-06-17 设计创作，主要内容包括：本发明提供一种具有改善畜禽肠道功能的菌-酶融合发酵培养物及其制备方法,属于生物饲料制备领域。其特征在于将不同质量份的豆粕、玉米、麸皮、稻壳粉、糖蜜作为发酵底物,优化复合益生菌和复合酶制剂的配方组合,采用菌-酶融合发酵方式而获得。本发明制备方法操作简单,易于实现,成本低,制备获得的发酵培养物有益菌及代谢产物丰富,具有促进动物采食,提高畜禽生产性能、降低肠道腹泻等疾病等的功能。(The invention provides a bacterium-enzyme fusion fermentation culture with an intestinal function of improving livestock and poultry and a preparation method thereof, belonging to the field of biological feed preparation. The method is characterized in that bean pulp, corn, bran, rice hull powder and molasses with different mass parts are used as fermentation substrates, the formula combination of the composite probiotics and the composite enzyme preparation is optimized, and the fermentation is achieved by adopting a bacterium-enzyme fusion fermentation mode. The preparation method disclosed by the invention is simple to operate, easy to implement and low in cost, and the prepared fermentation culture is rich in beneficial bacteria and metabolites, and has the functions of promoting animal feeding, improving the production performance of livestock and poultry, reducing diseases such as intestinal diarrhea and the like.)

1. A bacteria-enzyme fusion fermentation culture for improving intestinal functions of livestock and poultry is characterized in that: the fusion fermentation culture comprises the following components in parts by weight: 50-150 parts of soybean meal, 60-240 parts of corn, 120-380 parts of bran, 130-400 parts of rice hull powder, 30-130 parts of molasses, 1-4 parts of composite probiotic powder, 1-5 parts of composite enzyme powder and 340 parts of water 270-containing materials;

preferably, the composite probiotic powder consists of saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, lactobacillus acidophilus and enterococcus faecium; the compound enzyme powder consists of xylanase, beta-glucanase, cellulase, acid protease, neutral protease and pectinase.

2. The fused fermentation culture of claim 1, wherein: the fusion fermentation culture comprises the following components in parts by weight: 60-120 parts of soybean meal, 80-200 parts of corn, 140-300 parts of bran, 150-340 parts of rice hull powder, 60-110 parts of molasses, 1-3 parts of composite probiotic powder, 2-4 parts of composite enzyme powder and 320 parts of water 280-doped materials;

further preferably, in the composite probiotic powder, 0.2-1 part of saccharomyces cerevisiae, 0.1-0.8 part of bacillus subtilis, 0.1-0.8 part of bacillus licheniformis, 0.1-0.8 part of lactobacillus plantarum, 0.1-0.8 part of lactobacillus acidophilus and 0.1-0.8 part of enterococcus faecium are added;

further preferably, in the compound enzyme powder, 0.1-0.8 part of xylanase, 0.2-1.2 parts of beta-glucanase, 0.1-1 part of cellulase, 0.1-1 part of acid protease, 0.1-1 part of neutral protease and 0.1-0.8 part of pectinase are included.

3. The fusion fermentation culture of claim 2, wherein the total viable count of the composite probiotic powder is 2.0 × 10⁸～5.0×10¹¹cfu/g, wherein the viable count of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus and enterococcus faecium is 1.0 × 10⁶～3.0×10¹⁰cfu/g。

4. The fused fermentation culture of claim 2, wherein: in the compound enzyme powder, 3000-24000U/g of xylanase, 1000-6000U/g of beta-glucanase, 200-2000U/g of cellulase, 3000-30000U/g of acid protease, 5000-50000U/g of neutral protease and 3000-24000 ten thousand U/g of pectinase are contained.

5. A process for the preparation of a confluent fermentation culture according to claims 1-4, characterized in that: the method comprises the following steps:

1) sieving the soybean meal, the corn, the bran and the rice hull powder by using an analysis sieve, wherein the mesh number of sieve pores is 20-40 meshes;

2) weighing the composite probiotic powder according to a proportion, dissolving the composite probiotic powder with 10-20 times of water, and activating for later use;

3) weighing the compound enzyme powder according to a proportion, dissolving the compound enzyme powder with 10-20 times of water, and activating for later use;

4) weighing molasses according to a proportion, and diluting the molasses with the rest water for later use;

5) cleaning and disinfecting fermentation mixing equipment and a field;

6) weighing the soybean meal, the corn, the bran and the rice hull powder according to a certain proportion, placing the mixture into a mixer, and mixing for 3-8 minutes;

7) spraying the diluted molasses in the step 4) into the mixer in the step 6), and continuously mixing for 3-10 minutes;

8) spraying the composite probiotic liquid in the step 2) and the composite enzyme liquid in the step 3) into the mixer in the step 6), and continuously mixing for 5-10 minutes;

9) and (3) putting the uniformly mixed mixture in the mixer in the step 6) into a fermentation bag, placing the fermentation bag into a fermentation room at the temperature of 28-34 ℃, and fermenting for 72-120 hours to obtain the culture.

6. The method of claim 5, wherein: the water temperature for activating the composite probiotics is 28-35 ℃, and the activation time is 1.5-2.5 hours.

7. The method of claim 5, wherein: the temperature of the activating water of the compound enzyme is 32-42 ℃, and the activating time is 1-2 hours.

8. Use of the fused fermentation culture according to any one of claims 1 to 4 and the fused fermentation culture obtained by the production method according to any one of claims 5 to 7 in livestock.

9. The use of claim 8, wherein: the application can be used in any stage of livestock and poultry, and can be specifically represented by any one of the following 1) and 2):

1) the application in improving daily feed intake and/or daily gain of livestock and poultry;

2) can be used for reducing intestinal diseases such as diarrhea in livestock and poultry.

Technical Field

The invention relates to a bacterium-enzyme fusion fermentation culture with an intestinal function of improving livestock and poultry and a preparation method thereof, belonging to the field of biological feed preparation.

Background

The Antibacterial Growth Promoters (AGPs) have obvious effects of protecting the health of animals, improving the production performance, reducing the production cost and the like, and bring huge economic benefits to the breeding industry. However, the serious problems of pathogenic bacteria drug resistance, drug residues, environmental pollution and the like caused by long-term use of AGPs are increasingly prominent, and the health and safety of human beings are endangered. Therefore, the resistance inhibition in the feed becomes necessary. China has already specified, and the use of antibiotic growth promoters in the feed is prohibited since 1 month 7 in 2020. However, the problems of low intestinal health of animals, reduced production performance and increased mortality rate are increasingly highlighted due to the banning of feed antibiotics. Therefore, how to effectively solve the breeding problem caused by antibiotics is a difficult and necessary scientific research task at present.

Intestinal health determines the level of overall function and productivity of the animal, and ensuring intestinal health is the first major task of breeding. The biological fermentation feed or culture is obtained by processing raw materials by using microorganisms, can remove some antigen substances in the raw materials, degrades partial macromolecular substances such as polysaccharide, protein, fat and the like through the metabolic action of the microorganisms, generates micromolecular substances such as organic acid, soluble polypeptide and the like, and has important effect on the growth and development of animals. More importantly, after the probiotics in the fermentation culture enter the intestinal tracts of animals, an anaerobic environment can be caused by consuming oxygen in the intestinal tracts, so that the ecological balance of the intestinal tracts is maintained; moreover, the probiotics can generate various substances with antagonistic action on intestinal pathogenic bacteria, and promote intestinal health.

At present, the biological fermentation feed has no unified standard, the products on the market are relatively disordered, and the microbial fermentation single raw material is mainly used, such as fermented soybean meal and the like. The fermentation aims at eliminating antigen and improving the utilization rate of feed. The fermentation technology mainly comprises the steps of stacking solid for fermentation and drying, and the method is high in cost, low in probiotic activity and not in line with the actual requirements of a farm. Therefore, development and exploration of fermentation methods of different fermentation substrates are urgently needed to improve the generation rate of metabolites so as to adapt to the existing culture.

Disclosure of Invention

Based on the problems, the invention designs the formulas of probiotics and complex enzyme by screening different fermentation substrate combinations, and adopts advanced bacteria-enzyme fusion fermentation technology and proper fermentation conditions to obtain a high-performance microbial fermentation culture product.

In a first aspect, the invention provides a bacterium-enzyme fusion fermentation culture with the function of improving the intestinal tract function of livestock and poultry.

The fusion fermentation culture comprises the following components in parts by weight: 50-150 parts of soybean meal, 60-240 parts of corn, 120-380 parts of bran, 130-400 parts of rice hull powder, 30-130 parts of molasses, 1-4 parts of composite probiotic powder, 1-5 parts of composite enzyme powder and 270-340 parts of water.

Preferably, 60-120 parts of soybean meal, 80-200 parts of corn, 140-300 parts of bran, 150-340 parts of rice hull powder, 60-110 parts of molasses, 1-3 parts of composite probiotic powder, 2-4 parts of composite enzyme powder and 320 parts of water 280-doped materials.

In a specific embodiment, the composite probiotic powder comprises 0.2-1 part of saccharomyces cerevisiae, 0.1-0.8 part of bacillus subtilis, 0.1-0.8 part of bacillus licheniformis, 0.1-0.8 part of lactobacillus plantarum and 0.1-0.8 part of enterococcus faecium.

In a specific embodiment, the compound enzyme powder comprises 0.1-0.8 part of xylanase, 0.2-1.2 parts of beta-glucanase, 0.1-1 part of cellulase, 0.1-1 part of acid protease, 0.1-1 part of neutral protease and 0.1-0.8 part of pectinase.

In a specific embodiment, the total viable count of the composite probiotic powder is 2.0 × 10⁸～5.0×10¹¹cfu/g, wherein the viable count of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus and enterococcus faecium is 1.0 × 10⁶～3.0×10¹⁰cfu/g。

In a specific embodiment, the compound enzyme powder contains 3000-24000U/g xylanase, 1000-6000U/g beta-glucanase, 200-2000U/g cellulase, 3000-30000U/g acid protease, 5000-50000U/g neutral protease and 3000-24000 ten thousand U/g pectinase.

In a second aspect, the present invention provides a method for preparing a fermentation culture according to the first aspect, comprising the steps of:

1) sieving the soybean meal, the corn, the bran and the rice hull powder by using an analysis sieve, wherein the mesh number of sieve pores is 20-40 meshes;

2) weighing the composite probiotic powder according to a proportion, dissolving the composite probiotic powder with 10-20 times of water, and activating for later use;

3) weighing the compound enzyme powder according to a proportion, dissolving the compound enzyme powder with 10-20 times of water, and activating for later use;

4) weighing molasses according to a proportion, and diluting the molasses with the rest water for later use;

5) cleaning and disinfecting fermentation mixing equipment and a field;

6) weighing the soybean meal, the corn, the bran and the rice hull powder according to a certain proportion, placing the mixture into a mixer, and mixing for 3-8 minutes;

7) spraying the diluted molasses in the step 4) into the mixer in the step 6), and continuously mixing for 3-10 minutes;

8) spraying the composite probiotic liquid in the step 2) and the composite enzyme liquid in the step 3) into the mixer in the step 6), and continuously mixing for 5-10 minutes;

9) and (3) putting the uniformly mixed mixture in the mixer in the step 6) into a fermentation bag, placing the fermentation bag into a fermentation room at the temperature of 28-34 ℃, and fermenting for 72-120 hours to obtain the culture.

The fermentation bag adopts a novel fermentation feed bag, a patent product (patent publication No. CN207903051U), and at least one exhaust heat sealing structure is directly arranged on the bag body by utilizing a heat sealing technology. The fermentation bag is simple to manufacture and low in cost, can greatly reduce the risk of being polluted by mould near the exhaust valve and the phenomenon of bag expansion and breakage, and improves the fermentation success rate.

Preferably, the temperature of the activated water of the composite probiotics is 28-35 ℃, and the activation time is 1.5-2.5 hours.

Preferably, the temperature of the water for activating the composite enzyme is 32-42 ℃, and the activation time is 1-2 hours

In a third aspect, the invention provides the use of the fermentation culture of the first aspect and the fermentation culture prepared in the second aspect in livestock and poultry. The application can be used in any stage of livestock and poultry, and can be specifically represented by any one of the following 1) and 2):

1) the application of the composition in improving daily feed intake and/or daily gain of livestock and poultry;

2) and application in reducing intestinal tract diseases such as diarrhea of livestock and poultry.

The invention has the beneficial effects that:

1) the invention has excellent matching of fermentation substrate raw materials and bacterial enzyme selection: in order to save cost, the preparation of fermentation substrate is carried out by utilizing local raw material resources. The analysis of nutrients, antigens and other components is carried out through the formula of the fermentation substrate raw materials, and the complex enzyme and the complex bacteria are reasonably prepared by combining the physiological characteristics of animal intestinal tracts. So as to achieve the purpose of optimal fermentation effect.

2) The application of the bacterial enzyme fusion fermentation technology of the invention is as follows: in the process of bacterial enzyme fusion fermentation, heat generated by bacterial metabolism is beneficial to the enzymolysis reaction, the enzymolysis product can provide nutrition for bacterial reproduction, and the enzymatic reaction and the fermentation reaction are synergistic, so that more active metabolites are beneficial to being produced, and the actual using effect is improved.

3) The invention adopts a novel fermentation feed bag for fermentation: the culture obtained by fermenting the fermentation bag does not need a drying process, so that the activity of a large amount of beneficial live bacteria and metabolites can be completely reserved, and the effect of intestinal health care is improved. Compare check valve fermentation bag, the risk that novel fermentation feed bag can be very big near reduction discharge valve by the mould pollution and the broken bag phenomenon that rises improve the fermentation success rate.

4) The invention has simple and practical preparation: the product of the invention has simple preparation process, low investment and high return, and meets the requirements of farms.

5) The product has prominent testing effect: the product is detected to be rich in biological active substances such as probiotics, lactic acid, oligosaccharide, enzyme preparation, functional small peptide, unknown growth factors and the like. The product has outstanding effects on improving animal feeding, promoting animal growth and reducing intestinal tract diseases.

Drawings

FIG. 1: a flow chart of a preparation method of a bacteria-enzyme fusion fermentation culture for improving the intestinal function of livestock and poultry.

Detailed Description

The present invention will be further illustrated with reference to the following specific examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified.