阅读说明：本技术 一种保肝护胃的复合微生物制剂 (Compound microbial preparation for protecting liver and stomach ) 是由 王东荣 于 2020-05-26 设计创作，主要内容包括：本发明公开了一种保肝护胃的复合微生物制剂,其特征在于：该微生物制剂的主要成分包括酪酸梭菌、产朊假丝酵母、亮氨酸、乳酸菌、甘氨酸、丙氨酸；该微生物制剂具体制备方法,包括以下步骤：步骤一：以酪酸梭菌与95％的水混合形成培养基,将一定比例的酸性寡糖加入,增加酪酸梭菌的增殖效率,反应时长为4～7h,形成溶液A；本发明中,通过在酪酸梭菌中添加酸性寡糖,可以极大的提高酪酸梭菌的增殖效率,从而为后期使用中的保肝护胃提供基础,同时还能对肠道起到防护的作用,而亮氨酸、乳酸菌、甘氨酸、丙氨酸,进一步提高了使用的功能性,以及设计的抑制革兰氏阴性细菌,使得防护效果显著。(The invention discloses a compound microbial preparation for protecting liver and stomach, which is characterized in that: the microbial preparation mainly comprises Clostridium butyricum, Candida utilis, leucine, lactobacillus, glycine, and alanine; the preparation method of the microbial preparation specifically comprises the following steps: the method comprises the following steps: mixing clostridium butyricum with 95% water to form a culture medium, adding a certain proportion of acid oligosaccharide, increasing the proliferation efficiency of clostridium butyricum, and reacting for 4-7 h to form a solution A; according to the invention, the acidic oligosaccharide is added into the clostridium butyricum, so that the proliferation efficiency of the clostridium butyricum can be greatly improved, a foundation is provided for protecting the liver and the stomach in later use, and the intestinal tract can be protected, and the leucine, the lactic acid bacteria, the glycine and the alanine further improve the use functionality and inhibit gram-negative bacteria, so that the protection effect is obvious.)

1. A compound microbial preparation for protecting liver and stomach is characterized in that: the microbial preparation mainly comprises Clostridium butyricum, Candida utilis, leucine, lactobacillus, glycine, and alanine; the preparation method of the microbial preparation specifically comprises the following steps:

the method comprises the following steps: mixing clostridium butyricum with 95% water to form a culture medium, adding a certain proportion of acid oligosaccharide, increasing the proliferation efficiency of clostridium butyricum, and reacting for 4-7 h to form a solution A;

step two: preparing a liquid culture medium by using 1-2% of peanut cake powder, 2-3% of starch, 3-4% of molasses and 85-90% of water in mass ratio, placing the candida utilis after high-temperature sterilization, stirring for a certain time, and standing for 30-35 hours to prepare candida utilis fermentation liquor, namely solution B;

step three: mixing and stirring leucine, lactic acid bacteria, glycine and alanine according to a ratio, and standing for 1-2 hours to form a solution C;

step four: heating the solution A, the solution B and the solution C, and mixing and stirring at a heating temperature to obtain a solution D;

step five: and standing the solution D for 30-50 h, removing the upper part liquid of the solution D which forms a layer, and forming the compound microbial preparation in the rest.

2. The complex microbial preparation for protecting liver and stomach according to claim 1, wherein: the content of the acid oligosaccharide in the step one is 0.3-0.5%, wherein the acid oligosaccharide is powdery, and the particle size is 0.1-0.23 um.

3. The complex microbial preparation for protecting liver and stomach according to claim 1, wherein: and in the second step, sterilization is carried out at 70-100 ℃, and the stirring time is 3-5 min.

4. The complex microbial preparation for protecting liver and stomach according to claim 1, wherein: in the third step, the proportion of leucine, lactic acid bacteria, glycine and alanine is 1: 3: 3: 3.

5. the complex microbial preparation for protecting liver and stomach according to claim 1, wherein: the stirring time in the fourth step is 30-50 min, and the heating temperature is 80-85 ℃.

6. The complex microbial preparation for protecting liver and stomach according to claim 1, wherein: and inhibiting gram-negative bacteria, wherein the inhibiting gram-negative bacteria is added in the stirring of the third step.

7. The complex microbial preparation for protecting liver and stomach according to claim 1, wherein: and in the fifth step, the liquid on the upper part of the solution D accounts for 20-25% of the total liquid.

Technical Field

The invention belongs to the technical field of microbial preparations, and particularly relates to a compound microbial preparation for protecting liver and stomach.

Background

The microbial preparation product, commonly called as active probiotics, is used in various industries, has a wide range, can be used for decomposing organisms and can also be used for supplementing and protecting functions of human bodies.

The existing microbial preparation capable of protecting the liver and the stomach has low protection function, unobvious effect and single functionality in practical use, and has great limitation in practical use.

Disclosure of Invention

The invention aims to provide a compound microbial preparation for protecting liver and stomach, which solves the problems that the existing microbial preparation capable of protecting liver and stomach provided by the background technology is low in protection function, unobvious in effect, single in functionality and large in limitation in actual use.

In order to achieve the purpose, the invention provides the following technical scheme: a compound microbial preparation for protecting liver and stomach is characterized in that: the microbial preparation mainly comprises clostridium butyricum, candida utilis, leucine, lactic acid bacteria, glycine and alanine; the preparation method of the microbial preparation specifically comprises the following steps:

the method comprises the following steps: mixing clostridium butyricum with 95% water to form a culture medium, adding a certain proportion of acid oligosaccharide, increasing the proliferation efficiency of clostridium butyricum, and reacting for 4-7 h to form a solution A;

step two: preparing a liquid culture medium by using 1-2% of peanut cake powder, 2-3% of starch, 3-4% of molasses and 85-90% of water in mass ratio, placing the candida utilis after high-temperature sterilization, stirring for a certain time, and standing for 30-35 hours to prepare candida utilis fermentation liquor, namely solution B;

step three: mixing and stirring leucine, lactic acid bacteria, glycine and alanine according to a ratio, and standing for 1-2 hours to form a solution C;

step four: heating the solution A, the solution B and the solution C, and mixing and stirring at a heating temperature to obtain a solution D;

step five: and standing the solution D for 30-50 h, removing the upper part liquid of the solution D which is formed into a layered structure, and forming the composite microbial preparation in the rest way.

Preferably, the content of the acid oligosaccharide in the step one is 0.3-0.5%, wherein the acid oligosaccharide is in a powder shape, and the particle size is 0.1-0.23 um.

Preferably, the second step is carried out at 70-100 ℃ for 3-5 min.

Preferably, the ratio of leucine, lactic acid bacteria, glycine and alanine in the step three is 1: 3: 3: 3.

preferably, the stirring time in the fourth step is 30-50 min, and the heating temperature is 80-85 ℃.

Preferably, the method further comprises inhibiting gram-negative bacteria, wherein the gram-negative bacteria is added in the stirring of the third step.

Preferably, the liquid in the upper part of the solution D in the fifth step accounts for 20-25% of the total liquid.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the acidic oligosaccharide is added into the clostridium butyricum, so that the proliferation efficiency of the clostridium butyricum can be greatly improved, a foundation is provided for protecting the liver and the stomach in later use, and the intestinal tract can be protected, and the leucine, the lactic acid bacteria, the glycine and the alanine further improve the use function, and the designed inhibition of gram-negative bacteria can ensure that the protection effect is obvious.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments of the present invention