阅读说明：本技术 一种螺旋藻的高效活性成分制备方法及其应用 (Preparation method and application of efficient active ingredients of spirulina ) 是由 王志忠 巩东辉 季祥 李明 穆洁 智颖飙 孙君社 裴海生 张秀清 乔辰 栗淑媛 于 2020-05-09 设计创作，主要内容包括：本发明提供一种螺旋藻的高效活性成分制备方法及其应用,涉及螺旋藻活性成分提取领域。所述螺旋藻的高效活性成分制备方法,包括以下步骤：S1.将螺旋藻置于烘干箱内进行烘干处理,然后将烘干后的螺旋藻投入植物粉碎机进行粉碎,得到螺旋藻粉末,然后再将螺旋藻粉末使用70-90目筛网进行过滤筛分；S2.将筛分的螺旋藻粉末加入到蒸馏水中进行稀释,得到稀释液,然后向稀释液的内部加入溶菌酶以及巯基试剂搅拌混合,得到螺旋藻处理液,然后将螺旋藻处理液放置在温度为25-35℃环境中育养,育养时间为2-4h。通过对制备方法的合理设计,使得该制备方法在进行提取时,操作简单方便,同时能够进一步提高螺旋藻中藻蓝蛋白的提取效率。(The invention provides a preparation method and application of a high-efficiency active component of spirulina, and relates to the field of extraction of the active component of spirulina. The preparation method of the high-efficiency active component of the spirulina comprises the following steps: s1, placing spirulina in a drying box for drying, then putting the dried spirulina into a plant crusher for crushing to obtain spirulina powder, and then filtering and screening the spirulina powder by using a 70-90-mesh screen; s2, adding the screened spirulina powder into distilled water for dilution to obtain a diluent, adding lysozyme and a sulfhydryl reagent into the diluent, stirring and mixing to obtain a spirulina treatment solution, and then placing the spirulina treatment solution in an environment with the temperature of 25-35 ℃ for breeding for 2-4 hours. Through reasonable design of the preparation method, the preparation method is simple and convenient to operate when extraction is carried out, and meanwhile, the extraction efficiency of phycocyanin in spirulina can be further improved.)

1. A preparation method of high-efficiency active ingredients of spirulina is characterized by comprising the following steps: the method comprises the following steps:

s1, placing spirulina in a drying box for drying, then putting the dried spirulina into a plant crusher for crushing to obtain spirulina powder, and then filtering and screening the spirulina powder by using a 70-90-mesh screen;

s2, adding the screened spirulina powder into distilled water for dilution to obtain a diluent, adding lysozyme and a sulfhydryl reagent into the diluent, stirring and mixing to obtain a spirulina treatment solution, placing the spirulina treatment solution in an environment with the temperature of 25-35 ℃ for breeding for 2-4h, and simultaneously uniformly applying pressure to the inside of the spirulina treatment solution in the breeding process;

s3, centrifuging the cultured spirulina treatment liquid, and removing sediments after centrifugation by using a filter screen to obtain a supernatant solution;

s4, adding crystal ammonium sulfate into the obtained supernatant solution, slowly stirring and dissolving the crystal ammonium sulfate, adjusting the pH of the dissolved supernatant solution to 4-4.5, and then centrifuging the supernatant solution after pH adjustment to obtain phycocyanin slurry;

s5, placing the obtained phycocyanin slurry in a low-temperature vacuum for freeze drying treatment, and then obtaining the phycocyanin extract of the spirulina.

2. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the drying temperature in S1 is 30-35 deg.C, and the water content in the dried Spirulina is not higher than 5%.

3. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the ratio of spirulina powder to distilled water in S2 is 1: 35-40 g/ml.

4. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the addition amount of lysozym in S2 is 0.005-0.01 g/ml.

5. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the addition amount of the thiol reagent in S2 is 0.001 g/ml.

6. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the pressure applied in S2 is 25MPa/h and is increased to 50 MPa.

7. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the centrifugation treatment in S3 is 1000-1500 r/min.

8. The method for preparing a highly potent active ingredient of spirulina according to claim 1, wherein: wherein the solution added in the S4 for pH adjustment is malic acid, and the centrifugation treatment in the S4 is 1000-1500 r/min.

9. Use of a highly potent active ingredient of spirulina according to any of claims 1-8 in the research of antiviral activity and in preparations.

Technical Field

The invention relates to the technical field of extraction of active ingredients of spirulina, in particular to a preparation method and application of efficient active ingredients of spirulina.

Background

The spirulina has a filament length of 200-. The blue algae cells are colorless to the body, and the pigment is distributed in the pigment area outside the protoplast, and is blue-green. The surface of the algae body does not have a colloid sheath, the algae body is not easy to be attached by microorganisms, and bubbles are arranged in cells, so that the floating property is good. The top of the cell or the algae filament is not sharp, the transverse wall is not obvious, the top cell is round, the outer wall is not thickened, and the spirulina contains high phycocyanin.

Phycocyanin has extremely high erythropoietin activity, can directly stimulate the formation of CFU-E, has stimulation effect on bone marrow hematopoiesis, and has good antiviral activity, so the range of extraction products of phycocyanin in spirulina is continuously expanded at present, but at present, the process is more complicated when the phycocyanin is extracted, and the extraction rate of the phycocyanin is not high.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a preparation method of a high-efficiency active component of spirulina and application thereof, and solves the problems that the existing extraction process of phycocyanin is complex in operation and inconvenient for the development of the extraction process, and the extraction efficiency of the extraction process of phycocyanin is low and the cost is wasted.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: a method for preparing high-efficiency active ingredients of spirulina comprises the following steps:

s1, placing spirulina in a drying box for drying, then putting the dried spirulina into a plant crusher for crushing to obtain spirulina powder, and then filtering and screening the spirulina powder by using a 70-90-mesh screen;

s2, adding the screened spirulina powder into distilled water for dilution to obtain a diluent, adding lysozyme and a sulfhydryl reagent into the diluent, stirring and mixing to obtain a spirulina treatment solution, placing the spirulina treatment solution in an environment with the temperature of 25-35 ℃ for breeding for 2-4h, and simultaneously uniformly applying pressure to the inside of the spirulina treatment solution in the breeding process;

s3, centrifuging the cultured spirulina treatment liquid, and removing sediments after centrifugation by using a filter screen to obtain a supernatant solution;

s4, adding crystal ammonium sulfate into the obtained supernatant solution, slowly stirring and dissolving the crystal ammonium sulfate, adjusting the pH of the dissolved supernatant solution to 4-4.5, and then centrifuging the supernatant solution after pH adjustment to obtain phycocyanin slurry;

s5, placing the obtained phycocyanin slurry in a low-temperature vacuum for freeze drying treatment, and then obtaining the phycocyanin extract of the spirulina.

Preferably, the temperature of the drying treatment in S1 is 30-35 deg.C, and the water content in the dried spirulina is not higher than 5%.

Preferably, the ratio of the spirulina powder to the distilled water in the S2 is 1: 35-40 g/ml.

Preferably, the addition amount of the lysozym in S2 is 0.005-0.01 g/ml.

Preferably, the mercapto agent is added in an amount of 0.001g/ml in S2.

Preferably, wherein the pressure applied in S2 is 25MPa/h, the pressure is increased to 50 MPa.

Preferably, the centrifugation treatment in S3 is 1000-1500 r/min.

Preferably, the solution added in the S4 for pH adjustment is malic acid, and the centrifugation treatment in the S4 is 1000-1500 r/min.

Preferably, the preparation method of the high-efficiency active ingredient of the spirulina is applied to antiviral activity research and products.

(III) advantageous effects

The invention provides a preparation method and application of a high-efficiency active component of spirulina. The method has the following beneficial effects:

1. through reasonable design of the preparation method, the preparation method is simple and convenient to operate when extraction is carried out, and meanwhile, the extraction efficiency of phycocyanin in spirulina can be further improved.

2. Carry out the broken wall through enzymolysis method and appropriate amount sulfhydryl reagent to the cell and handle not only can handle a great number of cells simultaneously to greatly increased the speed of cell broken wall, can reduce the time of extraction process.

3. The quality of the phycocyanin after extraction can be improved by controlling the pH value in the process of extracting the phycocyanin.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.