阅读说明：本技术 一种含胭脂虫红的耐酸食用色素及其制备方法 (Acid-resistant edible pigment containing cochineal and preparation method thereof ) 是由 赵博谦 于 2021-05-31 设计创作，主要内容包括：本发明涉及食用色素领域,提供一种含胭脂虫红的耐酸食用色素及其制备方法,用于解决胭脂虫红色素耐酸性差的问题。本发明提供的含胭脂虫红的耐酸食用色素,包括：胭脂虫红色素粉末3～8%,壳聚糖0.1～5%,乙二胺四乙酸二钠0.1～1%,苯甲酸钠0.1～1%,α-环状糊精0.1～3%,丙三醇30～50%,去离子水40～60%。提高了色素在酸性环境中保存率,提高了色素的热稳定性,并提高了色素的着色力。(The invention relates to the field of edible pigments, and provides an acid-resistant edible pigment containing cochineal and a preparation method thereof, which are used for solving the problem of poor acid resistance of the cochineal pigment. The invention provides an acid-resistant edible pigment containing cochineal red, which comprises the following components: 3-8% of cochineal red pigment powder, 0.1-5% of chitosan, 0.1-1% of ethylene diamine tetraacetic acid disodium, 0.1-1% of sodium benzoate, 0.1-3% of alpha-cyclodextrin, 30-50% of glycerol and 40-60% of deionized water. The preservation rate of the pigment in an acidic environment is improved, the thermal stability of the pigment is improved, and the tinting strength of the pigment is improved.)

1. An acid-resistant edible pigment comprising cochineal red, comprising: 3-8% of cochineal red pigment powder, 0.1-5% of chitosan, 0.1-1% of ethylene diamine tetraacetic acid disodium, 0.1-1% of sodium benzoate, 0.1-3% of alpha-cyclodextrin, 30-50% of glycerol and 40-60% of deionized water.

2. The acid resistant food color comprising cochineal color of claim 1, comprising: 5-8% of cochineal red pigment powder, 3-5% of chitosan, 0.5-1% of ethylene diamine tetraacetic acid disodium, 0.5-1% of sodium benzoate, 2-3% of alpha-cyclodextrin, 40-50% of glycerol and 49-60% of deionized water.

3. The acid resistant food color comprising cochineal color of claim 2, comprising: 5% of cochineal red pigment powder, 3% of chitosan, 0.5% of ethylene diamine tetraacetic acid disodium, 0.5% of sodium benzoate, 2% of alpha-cyclodextrin, 40% of glycerol and 49% of deionized water.

4. A preparation method of acid-resistant edible pigment containing cochineal red is characterized by comprising the following steps:

adding disodium ethylene diamine tetraacetate, sodium benzoate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

5. The acid-resistant edible pigment containing cochineal red according to claim 4, wherein the emulsifying and dispersing equipment is a high-shear homogeneous emulsifying pump, the power is 5-10 KW, and the treatment amount is as follows: 0-16T/H, rotating speed: 2000-3000 r/min, rotor diameter: 100-200 mm.

6. The acid resistant edible color containing cochineal color according to claim 4, wherein the emulsifying and dispersing equipment is a high shear homogeneous emulsifying pump with a power of 7.5KW, and the processing amount is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm.

7. The acid resistant edible color comprising cochineal color according to claim 4, wherein the emulsification pump comprises three stator and rotor stages.

8. The acid resistant edible pigment containing cochineal red according to claim 4, wherein the filtering is performed by using a 100-300 mesh screen.

9. The acid resistant edible color comprising cochineal color of claim 4, wherein the emulsion pump operates in a down-draft cycle.

Technical Field

The invention relates to the field of edible pigments, in particular to an acid-resistant edible pigment containing cochineal red.

Background

The cochineal pigment is a natural edible pigment, is extracted from female cochineal bodies growing in different regions and on different types of cactus, is pink to purple in color, has the advantages of safety, no toxicity, good colorability and the like, and has a good application prospect. However, because the cochineal pigment has poor acid resistance, when used in acidic foods (pH < 4.0), precipitation and discoloration can occur, and the use of the cochineal pigment in food technology is limited to a certain extent.

Disclosure of Invention

The invention solves the technical problem of poor acid resistance of cochineal color, and provides an acid-resistant edible color containing cochineal color.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

an acid resistant food color comprising cochineal red, comprising: 3-8% of cochineal red pigment powder, 0.1-5% of chitosan, 0.1-1% of ethylene diamine tetraacetic acid disodium, 0.1-1% of sodium benzoate, 0.1-3% of alpha-cyclodextrin, 30-50% of glycerol and 40-60% of deionized water.

Compounding cochineal red with alpha-cyclodextrin, chitosan, glycerol, sodium benzoate, disodium ethylene diamine tetraacetate, etc. to obtain red pigment.

The preservation rate of the pigment in an acidic environment is improved, the thermal stability of the pigment is improved, and the tinting strength of the pigment is improved.

Preferably, the method comprises the following steps: 5-8% of cochineal red pigment powder, 3-5% of chitosan, 0.5-1% of ethylene diamine tetraacetic acid disodium, 0.5-1% of sodium benzoate, 2-3% of alpha-cyclodextrin, 40-50% of glycerol and 49-60% of deionized water.

Preferably, the method comprises the following steps: 5% of cochineal red pigment powder, 3% of chitosan, 0.5% of ethylene diamine tetraacetic acid disodium, 0.5% of sodium benzoate, 2% of alpha-cyclodextrin, 40% of glycerol and 49% of deionized water.

The chitosan has strong hygroscopicity, good film forming property, air permeability and biocompatibility, has a protective effect on the cochineal color by utilizing the film forming property in the formula, and has a synergistic effect on the aspect of improving the stability of the cochineal color when being used together with the alpha-cyclodextrin.

The disodium ethylene diamine tetraacetate can be chelated with multivalent ions such as iron, copper, calcium, magnesium and the like to form a stable water-soluble complex, can be chelated with radioactive substances such as yttrium, zirconium, radium, plutonium and the like, and has an antioxidation effect, so that the influence of common metal ions in food on cochineal pigment can be avoided in the product.

Sodium benzoate is used as antiseptic.

The alpha-cyclodextrin has good water solubility and a cylindrical hollow structure, can envelop cochineal molecules, and improves the stability of the cochineal molecules to light, heat and oxygen.

Glycerol is used as carrier solvent and has moisture keeping effect.

Deionized water is the carrier solvent.

A method for preparing acid-resistant edible pigment containing cochineal comprises:

adding disodium ethylene diamine tetraacetate, sodium benzoate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red. Effectively improving the stability of the cochineal under the acidic condition.

Preferably, the emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 5-10 KW, and the treatment capacity is as follows: 0-16T/H, rotating speed: 2000-3000 r/min, rotor diameter: 100-200 mm.

Preferably, the emulsifying and dispersing equipment is a high-shear homogeneous emulsifying pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm.

Preferably, the emulsification pump adopts three-stage stators and rotors.

Preferably, the filtering is performed by using a 100-300-mesh screen.

Preferably, the emulsion pump operates in a down-draft cycle.

Compared with the prior art, the invention has the beneficial effects that: the preservation rate of the pigment in an acidic environment is improved, the thermal stability of the pigment is improved, and the tinting strength of the pigment is improved; no precipitation in the environment with pH less than 3.0, unchanged color tone and high stability.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.

Example 1

An acid resistant food color comprising cochineal red, comprising: 500g of cochineal red pigment powder, 300g of chitosan, 50g of ethylene diamine tetraacetic acid disodium, 50g of sodium benzoate, 200g of alpha-cyclodextrin, 4000g of glycerol and 4900g of deionized water.

The preparation method comprises the following steps:

adding disodium ethylene diamine tetraacetate, sodium benzoate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Compounding cochineal red with alpha-cyclodextrin, chitosan, glycerol, sodium benzoate, disodium ethylene diamine tetraacetate, etc. to obtain red pigment. The preservation rate of the pigment in an acidic environment is improved, the thermal stability of the pigment is improved, and the tinting strength of the pigment is improved. Effectively improving the stability of the cochineal under the acidic condition.

Comparative example 1

An acid resistant food color comprising cochineal red, comprising: the formula comprises 500g of cochineal red pigment powder, 50g of ethylene diamine tetraacetic acid disodium, 50g of sodium benzoate, 200g of alpha-cyclodextrin, 4000g of glycerol and 5200g of deionized water.

The preparation method comprises the following steps:

adding disodium ethylene diamine tetraacetate, sodium benzoate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Comparative example 2

An acid resistant food color comprising cochineal red, comprising: 500g of cochineal red pigment powder, 300g of chitosan, 50g of sodium benzoate, 200g of alpha-cyclodextrin, 4000g of glycerol and 5050g of deionized water.

The preparation method comprises the following steps:

adding sodium benzoate and alpha-cyclodextrin into deionized water, and mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Comparative example 3

An acid resistant food color comprising cochineal red, comprising: 500g of cochineal color powder, 300g of chitosan, 50g of ethylene diamine tetraacetic acid, 200g of alpha-cyclodextrin, 4000g of glycerol and 5050g of deionized water.

The preparation method comprises the following steps:

adding disodium ethylene diamine tetraacetate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Comparative example 4

An acid resistant food color comprising cochineal red, comprising: 500g of cochineal red pigment powder, 300g of chitosan, 50g of ethylene diamine tetraacetic acid disodium, 50g of sodium benzoate, 4000g of glycerol and 5200g of deionized water.

The preparation method comprises the following steps:

adding disodium ethylene diamine tetraacetate and sodium benzoate into deionized water and uniformly mixing;

adding cochineal pigment and glycerol, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Comparative example 5

An acid resistant food color comprising cochineal red, comprising: 500g of cochineal red pigment powder, 300g of chitosan, 50g of ethylene diamine tetraacetic acid, 50g of sodium benzoate, 200g of alpha-cyclodextrin and 8900g of deionized water.

The preparation method comprises the following steps:

adding disodium ethylene diamine tetraacetate, sodium benzoate and alpha-cyclodextrin into deionized water and uniformly mixing;

adding cochineal pigment, and emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Comparative example 6

An acid resistant food color comprising cochineal red, comprising: cochineal color powder 500g, deionized water 9500 g.

The preparation method comprises the following steps:

adding cochineal pigment into deionized water, and mixing;

emulsifying and dispersing for 20-30 min;

filtering to obtain the acid-resistant edible pigment containing cochineal red.

The emulsification and dispersion equipment is a high-shear homogeneous emulsification pump, the power is 7.5KW, and the treatment capacity is as follows: 0-8T/H, rotating speed: 2500r/min, rotor diameter: 150 mm. The emulsification pump adopts three-level stators and rotors. The filtering adopts a 300-mesh screen. The working mode of the emulsion pump is a downward suction circulation mode.

Examples of the experiments

The acid-resistant edible pigment containing cochineal in example 1 and comparative examples 1-6 was taken, the pH value was adjusted to 3.0 in citric acid buffer solution, and the pigment was stored in a volumetric flask at room temperature for 1 hour, and the content of the pigment was measured and blanked.

Measurement of cochineal content: the absorbance of cochineal red at the maximum absorption wavelength (about 494 nm) was measured by a spectrophotometer method and the content was calculated.

Reagents and materials: 2 mol/L of hydrochloric acid solution, and the pigment of each embodiment is acidified by citric acid.

Instruments and devices: analytical balance: the precision is 0.1 mg; an ultraviolet-visible spectrophotometer.

Cochineal red assay procedure: accurately weighing 1 g (accurate to 0.0001 g) of cochineal (to-be-detected liquid), dissolving in 30mL of 2 mol/L hydrochloric acid solution, boiling, cooling to 20 ℃, transferring to a 1000 mL volumetric flask, and adding water to constant volume to scale. On a spectrophotometer, a 1 cm cuvette is used, water is used as a blank, the absorbance of the sample solution is measured at the maximum absorption peak of about 494 nm, the absorbance value of the measured solution is in the range of 0.650-0.750, otherwise, the sample weighing is required to be adjusted to prepare the sample solution.

And (4) calculating a result: cochineal content is calculated according to formula (A.1):

is the absorbance before adding acid and boiling,

the absorbance of formic acid after boiling was obtained.

The test results are based on the arithmetic mean of the results of the replicates. The absolute difference between two independent measurements obtained under repetitive conditions is not more than 1.0% of the arithmetic mean.

TABLE 1 pigment Retention ratio of each embodiment

The pigment retention of acid-resistant cochineal is seen, and example 1 is greatly improved compared to comparative example 6, widening the use in food technology and improving product competitiveness.

The inventor finds that the acid resistance of the cochineal red can be improved only by compounding the cochineal red with chitosan, disodium ethylenediamine tetraacetic acid, sodium benzoate, alpha-cyclodextrin, glycerol and the like on the basis of a large number of experiments, and any one of the cochineal red and the disodium edetate can not improve the acid resistance of the cochineal red.

The above detailed description is specific to possible embodiments of the present invention, and the above embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention should be included in the present claims.