阅读说明：本技术 一种茯苓配方颗粒的制备方法 (Preparation method of poria cocos formula granules ) 是由 胡辉 成焕波 徐名扬 刘源才 孙代华 黄海华 于 2020-04-20 设计创作，主要内容包括：本发明公开了一种提高茯苓配方颗粒产品质量的方法,先取茯苓丁采用NaOH碱液加热提取两次,过滤,调整pH值,然后减压浓缩、干燥,最后将茯苓干膏粉直接干法制成颗粒。本发明制得的茯苓配方颗粒无需添加任何辅料或茯苓粉末、药渣粉末等,且与饮片当量关系适中,临床调配量确保在1-1.5g范围内,茯苓多糖提取率较传统水煎法提高5倍以上,同时解决了茯苓配方颗粒多糖提取率偏低和当量关系偏高问题。(The invention discloses a method for improving the quality of a poria cocos formula particle product. The poria cocos formula particle prepared by the invention does not need to be added with any auxiliary materials or poria cocos powder, decoction dreg powder and the like, has moderate equivalent relation with decoction pieces, ensures that the clinical blending amount is in the range of 1-1.5g, improves the extraction rate of pachyman by more than 5 times compared with the traditional water decoction method, and solves the problems of low extraction rate and high equivalent relation of the poria cocos formula particle polysaccharide.)

1. The preparation method of the poria cocos formula granules is characterized by comprising the following steps:

1) adding 0.04-0.08 mol/L mol/mol NaOH alkali liquor into diced poria cocos, heating and extracting twice, wherein the alkali liquor added for the first time is 12-15 times of that of the diced poria cocos, soaking for a certain time, then heating and extracting for 30-40 minutes, the alkali liquor added for the second time is 10-12 times of that of the diced poria cocos, heating and extracting for 20-30 minutes, centrifuging each extracting solution while hot, then filtering by 120 meshes, combining the filtrates, and measuring the volume and pH value of the filtrates.

2) The filtrate is adjusted to weak acidity.

3) Concentrating the extracting solution obtained in the step 2) under reduced pressure to obtain an extract with the relative density of 1.15-1.30 (60-80 ℃), and then drying under reduced pressure in vacuum until the water content of the dry extract is less than or equal to 6%.

4) Drying the poria cocos dry paste powder in the step 3) to prepare granules, so as to obtain the poria cocos formula granules.

2. The method according to claim 1, wherein the soaking time in step 1) is 60 to 90 minutes.

3. The method according to claim 2, wherein the temperature of the heating extraction in the step 1) is 60 to 80 ℃.

4. The preparation method according to claim 1, wherein the first alkali extraction is performed in two steps, wherein 6-8 times of alkali is added in the first step for 10-15 minutes, and then 6-8 times of alkali is added in the second step for 10-15 minutes.

5. The method according to claim 1, wherein a proper amount of dilute hydrochloric acid is added to the filtrate obtained in step 1) in step 2), and the pH value is adjusted to 6-7.

Technical Field

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a preparation method of poria cocos formula granules.

Background

The Chinese medicine prescription granule is a series of granular products which are refined by adopting modern scientific technology and imitating the method of decocting traditional Chinese medicine decoction, and the traditional Chinese medicine decoction pieces are extracted, concentrated, dried, granulated and the like by taking water as a solvent.

However, with the development of the traditional Chinese medicine formula particles for 20 years and the modern research of traditional Chinese medicines, the source of the traditional Chinese medicines is very complicated, so that the method of completely imitating the decoction of the traditional Chinese medicine decoction is not suitable for all varieties. For example, the modern research proves that the effective component of the tangerine peel is hesperidin, but the transfer rate of the tangerine peel is less than 10 percent when the tangerine peel is extracted by water, and most effective substances are not extracted and effectively utilized; for example, mineral traditional Chinese medicines have low paste yield, so that the equivalent relationship of the finally obtained formula granules is over 1: 100, it was not possible to operate in actual blending.

The tuckahoe is a Chinese medicinal variety commonly used in clinic, modern researches show that the main active ingredient of the tuckahoe is polysaccharide substance, but the solubility of the tuckahoe in water is very low, the paste yield is only 1 to 2 percent by adopting a traditional water decoction method, the polysaccharide transfer rate is less than 5 percent, the equivalent relation between actual formula granules and decoction pieces reaches 1:50 to 100, the clinical recommended dosage of the tuckahoe decoction pieces is 10 to 15g, the actual formula granules are only 0.1 to 0.2g, and the actual packaging and blending are very difficult.

In order to solve the above problems, the prior art mainly adopts a method including two aspects: 1) the method mainly has the problems that a large amount of non-medicinal substances such as auxiliary materials, original poria medicinal material powder or poria residue powder and the like are taken by a patient, and fewer effective poria particles are actually taken, so that the medicinal effect and the health of the patient are influenced. 2) The method has the advantages that a certain amount of sodium carbonate solution is used for decoction instead of water decoction, so that the paste yield is improved, and the equivalent relation of Poria cocos formula particles is improved, but the method is poor in actual operability and is not suitable for large-scale production, and mainly shows that the sodium carbonate solution is too high in decoction temperature (close to 100 ℃), the decoction liquid is black, and effective substances can be damaged; the sodium carbonate alkali extract is seriously gelatinized and cannot be normally filtered, and the filtration is carried out after the dregs with high viscosity are separated by adopting a modern centrifugal technology; the sodium carbonate decoction is alkaline and is not consistent with the weak acidity of the tuckahoe formula particle extract, and the production and application are not suitable even if the conventional pH adjustment is adopted, because a large amount of carbon dioxide is generated after the alkali liquor extracted by the sodium carbonate is neutralized by adding acid, the reaction foaming is serious, and the operation is very difficult in actual large-scale production.

Disclosure of Invention

In order to solve the above disadvantages, the invention provides a preparation method of poria cocos formula granules, which comprises the following steps:

1) adding 0.04-0.08 mol/L mol/mol NaOH alkali liquor into diced poria cocos, heating and extracting twice, wherein the alkali liquor added for the first time is 12-15 times of that of the diced poria cocos, soaking for a certain time, then heating and extracting for 30-40 minutes, the alkali liquor added for the second time is 10-12 times of that of the diced poria cocos, heating and extracting for 20-30 minutes, centrifuging each extracting solution while hot, then filtering by 120 meshes, combining the filtrates, and measuring the volume and pH value of the filtrates.

The purpose of centrifugation in the step is to facilitate subsequent filtration, and as the extracted solution has certain viscosity after the pachymine extraction, the medicine residues are gelatinized, the centrifugation is firstly adopted to collect the centrifugate, and then the 120-mesh filtration method is adopted to effectively save the filtration time, and the filtration holes can be blocked by directly filtering the gelatinized medicine residues, so that the filtration is difficult.

2) The filtrate is adjusted to weak acidity.

3) Concentrating the extracting solution obtained in the step 2) under reduced pressure to obtain an extract with the relative density of 1.15-1.30 (60-80 ℃), and then drying under reduced pressure in vacuum until the water content of the dry extract is less than or equal to 6%.

4) Drying the poria cocos dry paste powder in the step 3) to prepare granules, so as to obtain the poria cocos formula granules.

Preferably, the soaking time in step 1) is 60 to 90 minutes. Soaking in the above time period to allow pachyman components to react with alkali solution sufficiently, and facilitate subsequent heating extraction, wherein too short time results in insufficient reaction, and too long time results in increased time cost.

Preferably, the temperature of the heating extraction in step 1) is 60-80 ℃. Under the condition of the temperature, the reaction of pachyman and alkali is moderate, the extract becomes viscous, gelatinized and black obviously when the temperature is too high, and polysaccharide substances are destroyed, and the extraction time needs to be prolonged when the temperature is too low.

Preferably, the first alkali liquor extraction is carried out in two steps, wherein 6-8 times of alkali liquor is added in the first step for fully extracting for 10-15 minutes, and then 6-8 times of alkali liquor is added in the second step for extracting for 10-15 minutes. The addition of the alkali liquor for the first time is beneficial to the full extraction between the tuckahoe and the alkali liquor, so that the pachyman content is improved.

Preferably, a proper amount of dilute hydrochloric acid is taken in the step 2) and added into the filtrate obtained in the step 1), and the pH value is adjusted to 6-7. The purpose of this step is to keep the weak acidity of the extract, the final chemical form being consistent with that of traditional water decoction.

The invention has the following beneficial effects:

the method of the invention ensures that the paste yield of the tuckahoe exceeds 10 percent, and the dry paste powder has good compressibility and hygroscopicity, so that the dry granulation can be normally carried out without adding any auxiliary material, the equivalent relation of the final formula particle finished product is moderate on the premise of ensuring that no auxiliary material is added, and the clinical blending amount is ensured to be within the range of 1-1.5 g. Secondly, compared with the traditional method, the extraction rate of the poria cocos formula particle polysaccharide obtained by the method is obviously improved by more than 5 times, so that the quality of the poria cocos formula particle product is obviously improved. The invention also solves the problems of low extraction rate and high equivalent relation of the poria cocos formula granular polysaccharide.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.