阅读说明：本技术 一种红托竹荪枝条菌种培育方法 (Dictyophora rubrovalvata branch strain cultivation method ) 是由 王跃霖 董如光 冯耀东 董建国 朱仕益 高云龙 徐冉 于 2020-06-01 设计创作，主要内容包括：本发明公开了一种红托竹荪枝条菌种培育方法,所述一种红托竹荪枝条菌种培育方法包括以下步骤：(1)原料选取、(2)枝条选取、(3)枝条处理、(4)玉米浆制备、(5)一次煮沸、(6)二次煮沸、(7)原料混合、(8)灭菌和(9)接种。本发明利用杨木枝条作为菌种的载体,在放置于培养基的时候可以四散发菌,使菌种可以呈现360°的生长,这种接种方式接种萌发吃料快,萌发一致稳定性好,可有效缩短发菌时间,菌龄一致,保证菌种活力,并且接种速度快,适应大规模生产,这种培育方式培育的枝条菌种既有固体菌种的稳定性,又有液体菌种的接种速度。(The invention discloses a Dictyophora rubrovalvata branch strain cultivation method, which comprises the following steps: (1) selecting raw materials, (2) selecting branches, (3) treating branches, (4) preparing corn steep liquor, (5) boiling for the first time, (6) boiling for the second time, (7) mixing raw materials, (8) sterilizing and (9) inoculating. The invention utilizes poplar branches as carriers of strains, can disperse the strains when being placed in a culture medium, enables the strains to grow at 360 degrees, has the advantages of fast inoculation, germination and feed intake, consistent germination and good stability, can effectively shorten the strain development time, has consistent strain age, ensures the strain activity, has high inoculation speed, is suitable for large-scale production, and has the stability of solid strains and the inoculation speed of liquid strains.)

1. A Dictyophora rubrovalvata branch strain cultivation method is characterized by comprising the following steps: the method for cultivating Dictyophora rubrovalvata branch strains comprises the following steps:

(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;

(2) selecting branches: selecting fresh poplar branches according to the production quantity, then cutting the fresh poplar branches into the sizes of 4-6mm in width, 4-6mm in thickness and 130-150mm in length for later use, and checking whether the poplar branches are damaged by insects or mildew or not before use;

(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;

(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1-2h for later use after dilution is completed;

(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;

(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;

(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;

(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;

(9) inoculation: selecting pure white, pollution-free and aging-free dictyophora rubrovolvata stock seeds, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seeds to a constant-temperature strain culture chamber for culture, and fully distributing hyphae after culturing for 75-90 days.

2. The method for cultivating Dictyophora rubrovalvata branch strains according to claim 1, wherein the method comprises the following steps: the dictyophora rubrovolvata branch strain is prepared from 78-82% of poplar branches, 14-16% of sawdust, 2.5-3% of corn flour, 1-2% of white granulated sugar, 0.8-1.2% of gypsum powder and 0.3-0.7% of monopotassium phosphate in parts by mass.

3. The method for cultivating Dictyophora rubrovalvata branch strains according to claim 1, wherein the method comprises the following steps: the size of the fungus bag is 15 x 33cm polypropylene fungus bag, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.

Technical Field

The invention relates to the technical field of edible fungi, in particular to a Dictyophora rubrovalvata branch strain cultivation method.

Background

Dictyophora rubrovalvata is a characteristic edible fungus in Guizhou, and can be artificially cultivated in a large scale. Dictyophora rubrovalvata is cultivated in China at present.

In the existing dictyophora rubrovolvata strain cultivation process, most of sawdust solid strains are used as main strains, the inoculation speed is low, the inoculation amount is difficult to control, the germination is slow, the pollution rate is high, the strains generally grow slowly from top to bottom in a culture medium, the growth speed is slow, the large-scale production is not suitable, the dictyophora rubrovolvata liquid strain production is unstable, and therefore, the dictyophora rubrovolvata branch strain cultivation method is provided.

Disclosure of Invention

The invention aims to provide a Dictyophora rubrovalvata branch strain cultivation method to solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a Dictyophora rubrovalvata branch strain cultivation method comprises the following steps:

(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;

(2) selecting branches: selecting fresh poplar branches according to the production quantity, then cutting the fresh poplar branches into the sizes of 4-6mm in width, 4-6mm in thickness and 130-150mm in length for later use, and checking whether the poplar branches are damaged by insects or mildew or not before use;

(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;

(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1-2h for later use after dilution is completed;

(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;

(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;

(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;

(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;

(9) inoculation: selecting pure white, pollution-free and aging-free dictyophora rubrovolvata stock seeds, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seeds to a constant-temperature strain culture chamber for culture, and fully distributing hyphae after culturing for 75-90 days.

Furthermore, the dictyophora rubrovolvata branch strain manufacturing raw materials comprise, by mass, 78-82% of poplar branches, 14-16% of sawdust, 2.5-3% of corn flour, 1-2% of white granulated sugar, 0.8-1.2% of gypsum powder and 0.3-0.7% of monopotassium phosphate.

Further, the size of the fungus bag is 15 x 33cm, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.

The invention utilizes poplar branches as carriers of strains, can disperse the strains when being placed in a culture medium, enables the strains to grow at 360 degrees, has the advantages of fast inoculation, germination and feed intake, consistent germination and good stability, can effectively shorten the strain development time, has consistent strain age, ensures the strain activity, has high inoculation speed, is suitable for large-scale production, and has the stability of solid strains and the inoculation speed of liquid strains.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.