阅读说明：本技术 一种聚赖氨酸制备方法及其制备装置 (Preparation method and preparation device of polylysine ) 是由 洪立芝 于 2020-05-12 设计创作，主要内容包括：本发明属于聚赖氨酸产品领域,具体是一种聚赖氨酸的制备方法及其制备装置；所述聚赖氨酸的制备方法包括选种、扩大培养、发酵制备以及过滤澄清；通过所述聚赖氨酸的制备方法生产的聚赖氨酸产品传代稳定,聚赖氨酸生产能力高效；另外通过相关实验发现,经所述聚赖氨酸的制备方法生产的聚赖氨酸产品抗菌能力强,保鲜效果好,安全性较高；更重要的是,生产聚赖氨酸产品的制备装置与传统的生物发酵装置相比,其能满足对发酵条件中的各个相关物理参数实现轻易控制调节,而且其设置的搅拌组件是通过电磁感应驱动原理进行,这样既避免在封盖上安装转轴,打开封盖不便,又能避免在制备装置底端穿过转轴,造成制备装置在发酵过程中容易发生渗漏的问题。(The invention belongs to the field of polylysine products, and particularly relates to a preparation method and a preparation device of polylysine; the preparation method of the polylysine comprises seed selection, enlarged culture, fermentation preparation and filtration clarification; the polylysine product produced by the preparation method of polylysine has stable passage and high polylysine production capacity; in addition, relevant experiments show that the polylysine product produced by the preparation method of the polylysine has strong antibacterial ability, good fresh-keeping effect and higher safety; more importantly, compared with the traditional biological fermentation device, the preparation device for producing the polylysine product can easily control and adjust various relevant physical parameters in fermentation conditions, and the stirring assembly arranged on the preparation device is driven by an electromagnetic induction driving principle, so that the problem that the leakage of the preparation device is easy to occur in the fermentation process due to the fact that a rotating shaft is installed on a sealing cover and the sealing cover is inconvenient to open is avoided, and the rotating shaft penetrates through the bottom end of the preparation device is also avoided.)

1. A method for preparing polylysine, which is characterized by comprising the following steps: selecting seeds, enlarging culture, fermenting preparation, filtering and clarifying;

the specific implementation mode of the seed selection step is as follows: selecting a mixed strain of streptomyces albus and ergot fungi as a polylysine strain;

the specific implementation mode of the amplification culture step is as follows: under the aseptic operation condition, transplanting the selected polylysine strain into a prepared culture dish for culture, wherein the culture dish is prepared by taking glucose as a carbon source, taking peptone as a nitrogen source and matching inorganic salt and growth factors; performing amplification culture on the polylysine strain on a culture dish for 3-5 times, wherein the time for each amplification culture is 4-6 d; the temperature of each amplification culture is controlled to be 23-29 ℃, and the culture humidity is kept at 60-65%;

the specific implementation mode of the fermentation preparation steps is as follows: adding the conventional fermentation culture solution wort used in the fermentation operation into a preparation device, adding the polylysine strain obtained in the step of enlarged culture, and fermenting for 2-4 days in the fermentation preparation device, wherein the fermentation temperature is kept at 30-40 ℃;

the specific implementation mode of the filtration and clarification is as follows: filtering the fermentation liquor obtained by the fermentation preparation device to remove filter residues to obtain filtrate, then carrying out centrifugal separation on the filtrate by a centrifugal machine, and screening out supernate on a centrifugal tube to separate out polylysine.

2. A manufacturing apparatus involved in the method for manufacturing polylysine according to claim 1, comprising: preparing a device body (9);

the upper end of the preparation device body (9) is provided with a sealing cover (10), the left side of the preparation device body (9) is provided with oxygen supply pipes (6) and a liquid level meter (8), the oxygen supply pipes (6) are communicated through a two-way pipe (7), and the preparation device body (9) is communicated with an oxygen tank (5) through the oxygen supply pipes (6); the right side of the preparation device body (9) is communicated with a booster pump (15) through a booster pipe (14); discharge pipes (16) are arranged on the left side and the right side of the lower end of the preparation device body (9), discharge valves are arranged on the discharge pipes (16), and the bottom end of the preparation device body (9) is fixedly arranged on the operation platform (4);

thermometer (18) and pressure gauge (20) are installed respectively to the inside upper end of preparation facilities body (9), and preparation facilities body (9) bottom inner wall left and right sides is equipped with resistance heating piece (17), installs sleeve (22) between resistance heating piece (17), and sleeve (22) upper end is equipped with stopper (21), and movable mounting has stirring subassembly (23) on sleeve (22), and sleeve (22) inside is equipped with electromagnetic drive subassembly.

3. The manufacturing apparatus according to claim 2, characterized in that: the sealing cover (10) and the preparation device body (9) are fixedly installed in a threaded interference fit mode, and when the sealing cover (10) is assembled with the preparation device body (9), a circle of rubber gasket (19) is installed at the upper end of the preparation device body (9).

4. The manufacturing apparatus according to claim 3, characterized in that: the upper end of the sealing cover (10) is provided with a thread sealing feeding plug (11), a pull handle (12) and a perspective window (13).

5. The manufacturing apparatus according to claim 2, characterized in that: stirring assembly (23) include permanent magnetism ring (25), puddler (26) and stirring leaf (27), and permanent magnetism ring (25) suit is on sleeve (22), and the installation is fixed with puddler (27) and puddler (26) respectively in the permanent magnetism ring (25) left and right sides, and the quantity of stirring leaf (27) and the quantity of puddler (26) are two, and two stirring leaves (27) and two puddlers (26) all distribute from top to bottom on permanent magnetism ring (25).

6. The manufacturing apparatus according to claim 2, characterized in that: the electromagnetic driving assembly comprises an electromagnetic block (24) and a motor (3), the electromagnetic block (24) is arranged inside the sleeve (22), and the lower end of the electromagnetic block (24) is connected with a driving shaft of the motor (3).

7. The manufacturing apparatus according to claim 6, wherein: the motor (3) is installed and fixed on the base (1), and the base (1) is fixedly connected with the operation table (4) through four brackets (2).

Technical Field

The invention relates to the field of polylysine products, in particular to a preparation method and a preparation device of polylysine.

Background

Spoilage of food causes significant economic losses and preventing spoilage is a concern of food scientists. At present, food preservatives are added in common preservative measures, the food preservatives are divided into chemical preservatives, natural preservatives and compound preservatives, the most widely used chemical preservatives are used, the chemical preservatives have certain side effects on human bodies although the use effects are obvious, the action time is long, the use effects are stable and continuous, and along with the gradual enhancement of health consciousness of people, the application of the natural preservatives and the compound preservatives can be wider and wider, and the market potential is huge.

Polylysine is a natural biological metabolic product, has good bactericidal capacity and thermal stability, and is a biological preservative with excellent preservative performance and great commercial potential. In addition, researchers also find that the polylysine is matched with other natural bacteriostatic agents to use, so that the polylysine has an obvious synergistic interaction effect and can improve the bacteriostatic ability of the polylysine.

In view of the above problems in the background art, the present invention is directed to a method for preparing polylysine and an apparatus for preparing the same.

Disclosure of Invention

The present invention is directed to a method and an apparatus for preparing polylysine, which solves the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for preparing polylysine, comprising the steps of: selecting seeds, enlarging culture, fermenting preparation, filtering and clarifying;

the specific implementation mode of the seed selection step is as follows: the mixed strain of streptomyces albus and ergot fungus is selected as the polylysine strain.

The specific implementation mode of the amplification culture step is as follows: under the aseptic operation condition, transplanting the selected polylysine strain into a prepared culture dish for culture, wherein the culture dish is prepared by taking glucose as a carbon source, taking peptone as a nitrogen source and matching inorganic salt and growth factors; performing amplification culture on the polylysine strain on a culture dish for 3-5 times, wherein the time for each amplification culture is 4-6 d; the temperature of each amplification culture is controlled to be 23-29 ℃, and the culture humidity is kept at 60-65%.

The specific implementation mode of the fermentation preparation steps is as follows: adding the conventional fermentation culture solution wort used in the fermentation operation into a fermentation preparation device, adding the polylysine strain obtained in the step of enlarged culture, and fermenting for 2-4 days in the fermentation preparation device, wherein the fermentation temperature is kept at 30-40 ℃.

The specific implementation mode of the filtration and clarification is as follows: filtering the fermentation liquor obtained by the fermentation preparation device to remove filter residues to obtain filtrate, then carrying out centrifugal separation on the filtrate by a centrifugal machine, and screening out supernate on a centrifugal tube to continuously separate out polylysine.

A manufacturing apparatus involved in a method for manufacturing polylysine, comprising: the device comprises a preparation device body, wherein a sealing cover is arranged at the upper end of the preparation device body, oxygen feeding pipes and a liquid level meter are arranged on the left side of the preparation device body, the oxygen feeding pipes are communicated through a two-way pipe, and the preparation device body is communicated with an oxygen tank through the oxygen feeding pipes; the right side of the preparation device body is communicated with a booster pump through a booster pipe; discharge pipes are arranged on the left side and the right side of the lower end of the preparation device body, discharge valves are arranged on the discharge pipes, and the bottom end of the preparation device body is fixedly arranged on an operation platform;

the upper end in the preparation device body is respectively provided with a thermometer and a pressure gauge, the left side and the right side of the inner wall of the bottom end of the preparation device body are provided with resistance heating blocks, a sleeve is arranged between the resistance heating blocks, the upper end of the sleeve is provided with a limiting block, a stirring assembly is movably arranged on the sleeve, and an electromagnetic driving assembly is arranged in the sleeve;

after the wort and polylysine strain mixed liquid is put into the preparation device body, the electromagnetic driving component drives the stirring component at the upper end of the sleeve to operate, the mixed liquid is stirred, so that the wort and the polylysine strain are uniformly and fully mixed, and the fermentation effect is improved; in addition, the temperature of the fermentation liquor is detected in real time by a thermometer in the fermentation process, and if the temperature is too low, the fermentation liquor can be electrified and heated by a resistance heating block; monitoring the internal pressure of the preparation device body in real time through a pressure gauge, and if the internal pressure of the preparation device body is too small, pressurizing by starting a booster pump; in addition, in order to improve the effect of aerobic fermentation, sufficient oxygen can be conveyed into the preparation device body through the oxygen tank in the fermentation process.

Compared with the prior art, the invention has the beneficial effects that:

the polylysine product produced by the preparation method of polylysine has easily controlled fermentation conditions, stable passage and high polylysine production capacity; in addition, relevant experiments show that the polylysine product produced by the preparation method of the polylysine has strong antibacterial ability, good fresh-keeping effect and higher safety;

more importantly, compared with the traditional biological fermentation device, the preparation device for producing the polylysine product can easily control and adjust various relevant physical parameters in fermentation conditions, and the stirring assembly arranged on the preparation device is driven by an electromagnetic induction driving principle, so that the problem that the leakage of the preparation device is easy to occur in the fermentation process due to the fact that a rotating shaft is installed on a sealing cover and the sealing cover is inconvenient to open is avoided, and the rotating shaft penetrates through the bottom end of the preparation device is also avoided.

Drawings

Fig. 1 is a schematic structural view of an apparatus for preparing polylysine according to an embodiment of the present invention.

Fig. 2 is a schematic view of the internal structure of an apparatus for preparing polylysine according to an embodiment of the present invention.

FIG. 3 is a schematic structural diagram of a stirring assembly of an apparatus for preparing polylysine according to an embodiment of the present invention.

In the figure: 1-base, 2-support, 3-motor, 4-operation platform, 5-oxygen tank, 6-oxygen feeding pipe, 7-two-way pipe, 8-level gauge, 9-preparation device body, 10-sealing cover, 11-screw thread sealing feeding plug, 12-pull handle, 13-perspective window, 14-booster pipe, 15-booster pump, 16-discharging pipe, 17-resistance heating block, 18-thermometer, 19-rubber gasket, 20-pressure gauge, 21-limiting block, 22-sleeve, 23-stirring component, 24-electromagnetic block, 25-permanent magnetic ring, 26-stirring rod and 27-stirring blade.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to specific embodiments.