阅读说明：本技术 一种抗肿瘤的中药复合物及其制备方法和应用 (Anti-tumor traditional Chinese medicine compound and preparation method and application thereof ) 是由 冯奕斌 陈可瑜 夏文 吴贵辉 陈红羽 冉娜 吴玉春 孙田甜 于 2019-11-13 设计创作，主要内容包括：本发明公开了一种抗肿瘤的中药复合物及其制备方法和应用。所述抗肿瘤的中药复合物,由下述原料制得,大丁草和红花龙胆；其制备方法为：将大丁草和红花龙胆混合后加提取剂提取,得提取液,将提取液浓缩干燥,得抗肿瘤的中药复合物；或将大丁草和红花龙胆分别加提取剂提取,分别得提取液,将提取液分别浓缩干燥后混合,得抗肿瘤的中药复合物；所述的抗肿瘤的中药复合物可应用于抗肿瘤药品和食品的制备。本发明可以显著抑制肝细胞癌和非小细胞肺癌的浸润和转移。(The invention discloses an anti-tumor traditional Chinese medicine compound and a preparation method and application thereof. The anti-tumor traditional Chinese medicine compound is prepared from the following raw materials, gerbera piloselloides and gentiana carthami; the preparation method comprises the following steps: mixing herba Erodii seu Geranii and radix Gentianae, extracting with extractant to obtain extractive solution, concentrating, and drying to obtain anti-tumor Chinese medicinal composition; or extracting herba Erodii seu Geranii and radix Gentianae with extractant respectively to obtain extractive solutions, respectively concentrating and drying the extractive solutions, and mixing to obtain anti-tumor Chinese medicinal composition; the anti-tumor traditional Chinese medicine compound can be applied to preparation of anti-tumor medicines and foods. The invention can obviously inhibit the infiltration and the metastasis of hepatocellular carcinoma and non-small cell lung cancer.)

1. An anti-tumor traditional Chinese medicine compound is characterized in that: the traditional Chinese medicine composition is prepared from the following raw materials, by weight, 1-10 parts of gerbera latifolia and 1-10 parts of gentiana carthami.

2. The anti-tumor traditional Chinese medicine compound according to claim 1, wherein: 3-8 parts of gerbera latifolia and 3-8 parts of safflower gentian.

3. The anti-tumor traditional Chinese medicine compound according to claim 1, wherein: the weight ratio of the gerbera piloselloides to the gentiana rubra is 1: 1.

4. The anti-neoplastic traditional Chinese medicine composition according to any one of claims 1 to 3, wherein: the anti-tumor traditional Chinese medicine compound contains 1% -3% of mangiferin.

5. The method for preparing an anti-tumor Chinese medicinal composition according to any one of claims 1 to 4, wherein: mixing herba Erodii seu Geranii and radix Gentianae, extracting with extractant to obtain extractive solution, concentrating, and drying to obtain anti-tumor Chinese medicinal composition; or extracting herba Erodii seu Geranii and radix Gentianae with extractant respectively to obtain extractive solutions, respectively concentrating, drying, and mixing to obtain anti-tumor Chinese medicinal composition; the extractant is water or ethanol solution, and the ethanol solution is 50-100% ethanol water solution; when the gerbera piloselloides and the gentiana rubra are mixed and extracted, the weight ratio of the extracting agent to the gerbera piloselloides and the gentiana rubra is 2-25:1, and when the gerbera piloselloides and the gentiana rubra are respectively extracted, the weight ratio of the extracting agent to the gerbera piloselloides or the gentiana rubra is 2-25: 1; the extraction temperature is 25-100 deg.C.

6. The method for preparing an anti-tumor Chinese medicinal composition according to claim 5, wherein the method comprises the following steps: the extraction is decoction extraction, the extraction temperature is 90-100 ℃, and the extraction time is 2-4 hours.

7. The method for preparing an anti-tumor Chinese medicinal composition according to claim 5, wherein the method comprises the following steps: pulverizing herba Erodii seu Geranii and radix Gentianae Carthami respectively, mixing, soaking in water 5-10 times the weight of herba Erodii seu Geranii and radix Gentianae Carthami for 12-24 hr, heating to 90-100 deg.C, heating for 0.5-2 hr, leaching, concentrating the obtained water extractive solution, and drying to obtain anti-tumor Chinese medicinal composition; or respectively crushing the gerbera and the gentiana rubra, respectively adding water to soak for 12-24h, adding 5-10 times of water by weight of the gerbera into the gerbera, adding 5-10 times of water by weight of the gentiana rubra into the gentiana rubra, respectively heating to 90-100 ℃, heating for 0.5-2 h to leach, respectively concentrating and drying the obtained water extract, and mixing to obtain the anti-tumor traditional Chinese medicine compound.

8. The use of the anti-tumor Chinese medicinal composition according to any one of claims 1 to 4 in the preparation of an anti-tumor medicament, wherein: the anti-tumor medicament is prepared into any oral or non-oral dosage form suitable for clinical use by taking an anti-tumor Chinese medicinal compound as a raw material and adding pharmaceutically acceptable auxiliary materials according to a conventional preparation process.

9. The use of the anti-tumor Chinese medicinal composition according to claim 8 in the preparation of anti-tumor medicaments, which is characterized in that: the tumor is liver cancer and lung cancer.

10. The use of the anti-tumor Chinese herbal compound according to any one of claims 1 to 4 in the preparation of anti-tumor food, wherein: the anti-tumor food is prepared by taking the anti-tumor traditional Chinese medicine compound as a raw material and adding conventional auxiliary materials for food production according to a conventional food production process.

11. The use of the anti-tumor traditional Chinese medicine compound according to claim 10 in the preparation of anti-tumor food, wherein: the food is a health-care product which is used alone or in combination with other anti-tumor treatment medicines.

12. The use of the anti-tumor traditional Chinese medicine compound according to claim 11 in the preparation of anti-tumor food, wherein the health care product is a health care product for inhibiting infiltration and invasion of cancer cells.

Technical Field

The invention relates to the technical field of anti-tumor traditional Chinese medicine compounds, in particular to an anti-tumor traditional Chinese medicine compound and a preparation method and application thereof.

Background

Hepatocellular carcinoma (HCC) is a common lethal malignancy, resulting in nearly 100 million deaths worldwide each year, with unpredictable early and poor prognosis of hepatocellular carcinoma leading to high mortality rates from liver cancer. Notably, only 30% of liver cancer patients are eligible for surgical treatment including liver transplantation. Lung cancer is the first common cancer ranked worldwide with high mortality. It is estimated that lung cancer accounts for 18.4% of cancer mortality. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, accounting for over 85% of the total incidence of lung cancer. The search for a new drug for inhibiting the growth and metastasis of liver cancer and lung cancer is imminent.

BL02 is prepared from 2 Chinese-medicinal materials including gerbera and gentiana carthami. The formulation is widely used in Yunnan and Guizhou provinces in China to treat cough and inflammatory reaction. The application and the curative effect of the compound in resisting tumors are not reported.

Disclosure of Invention

The invention aims to provide an anti-tumor traditional Chinese medicine compound and a preparation method and application thereof. The invention can obviously inhibit the infiltration and the metastasis of hepatocellular carcinoma and non-small cell lung cancer.

The technical scheme of the invention is as follows: an anti-tumor traditional Chinese medicine compound is prepared from the following raw materials, by weight, 1-10 parts of gerbera macrantha and 1-10 parts of gentiana carthami. (the weight parts in the invention can be weight units recognized in the medical field such as mg, g, kg, etc.)

In the anti-tumor traditional Chinese medicine compound, by weight, 3-8 parts of gerbera latifolia and 3-8 parts of safflower gentian are added.

In the anti-tumor traditional Chinese medicine compound, the weight ratio of the gerbera piloselloides and the gentiana rubra is 1: 1.

In the anti-tumor traditional Chinese medicine compound, the anti-tumor traditional Chinese medicine compound contains 1-3% of mangiferin.

The preparation method of the anti-tumor traditional Chinese medicine compound comprises the steps of mixing gerbera piloselloides and gentiana carthami, adding an extracting agent for extraction to obtain an extracting solution, concentrating and drying the extracting solution to obtain the anti-tumor traditional Chinese medicine compound; or extracting herba Erodii seu Geranii and radix Gentianae with extractant respectively to obtain extractive solutions, respectively concentrating, drying, and mixing to obtain anti-tumor Chinese medicinal composition; the extractant is water or ethanol solution, and the ethanol solution is 50-100% ethanol water solution; when the gerbera piloselloides and the gentiana rubra are mixed and extracted, the weight ratio of the extracting agent to the gerbera piloselloides and the gentiana rubra is 2-25:1, and when the gerbera piloselloides and the gentiana rubra are respectively extracted, the weight ratio of the extracting agent to the gerbera piloselloides or the gentiana rubra is 2-25: 1; the extraction temperature is 25-100 deg.C.

In the preparation method of the anti-tumor traditional Chinese medicine compound, the gerbera piloselloides and the gentiana carthami are respectively crushed and mixed, water with the weight 2-25 times that of the gerbera piloselloides and the gentiana carthami is added, the mixture is heated to 90-100 ℃ and is heated for 1-2 hours for extraction, and the obtained water extract is concentrated and dried to obtain the anti-tumor traditional Chinese medicine compound.

In the preparation method of the anti-tumor traditional Chinese medicine compound, the amount of the extracting agent is 15-25 times of the total amount of the gerbera piloselloides and the gentiana rubra.

In the preparation method of the anti-tumor traditional Chinese medicine compound, the extraction method is decoction extraction; the extraction temperature is 90-100 ℃, and the extraction time is 2-4 hours.

In the preparation method of the anti-tumor traditional Chinese medicine compound, the gerbera and the gentiana carthami are respectively crushed and then mixed, water is added for soaking for 12-24 hours, the weight of the water is 5-10 times of that of the gerbera and the gentiana carthami, the water is heated to 90-100 ℃, the water is heated for 0.5-2 hours for leaching, and the obtained water extract is concentrated and dried to obtain the anti-tumor traditional Chinese medicine compound; or respectively crushing the gerbera and the gentiana rubra, respectively adding water to soak for 12-24h, adding 5-10 times of water by weight of the gerbera into the gerbera, adding 5-10 times of water by weight of the gentiana rubra into the gentiana rubra, respectively heating to 90-100 ℃, heating for 0.5-2 h to leach, respectively concentrating and drying the obtained water extract, and mixing to obtain the anti-tumor traditional Chinese medicine compound.

In the preparation method of the anti-tumor traditional Chinese medicine compound, the gerbera piloselloides and the carthamus tinctorius are coarse pulverized medicinal powder.

The anti-tumor traditional Chinese medicine compound is used for preparing anti-tumor medicines, and the anti-tumor medicines are prepared into any oral or non-oral dosage form suitable for clinical use by taking the anti-tumor traditional Chinese medicine compound as a raw material and adding pharmaceutically acceptable auxiliary materials according to a conventional preparation process.

In the application of the anti-tumor traditional Chinese medicine compound in preparing the anti-tumor medicine, the anti-tumor medicine takes the anti-tumor traditional Chinese medicine compound as a raw material, pharmaceutically acceptable auxiliary materials and other traditional Chinese medicine extracts are added according to a conventional preparation process, and any oral or non-oral preparation suitable for clinical use is prepared.

In the application of the anti-tumor traditional Chinese medicine compound in preparing anti-tumor medicines, the anti-tumor medicines are anti-tumor medicines for inhibiting infiltration and invasion of cancer cells.

In the application of the anti-tumor traditional Chinese medicine compound in preparing anti-tumor medicines, the tumors are liver cancer and lung cancer.

The anti-tumor food is prepared by taking the anti-tumor traditional Chinese medicine compound as a raw material and adding conventional auxiliary materials for food production according to a conventional food production process.

The anti-tumor food is prepared by taking the anti-tumor traditional Chinese medicine compound as a raw material and adding conventional auxiliary materials for food production and other traditional Chinese medicine extracts according to a conventional food production process.

In the application of the anti-tumor traditional Chinese medicine compound in preparing anti-tumor food, the food is a health-care product, and the health-care product is used alone or combined with other anti-tumor treatment medicines.

In the application of the anti-tumor traditional Chinese medicine compound in preparing anti-tumor food, the health-care product is a health-care product for inhibiting infiltration and invasion of cancer cells.

Compared with the prior art, the anti-tumor traditional Chinese medicine compound has an inhibiting effect on the growth and metastasis of tumors. The anti-tumor traditional Chinese medicine compound can effectively inhibit the infiltration and growth of liver tumor to normal tissues, and after the anti-tumor traditional Chinese medicine compound is used for treating, the junction of the normal tissues and the liver cancer tissues becomes clearer. The anti-tumor traditional Chinese medicine compound can obviously inhibit lung metastasis of liver cancer. The anti-tumor traditional Chinese medicine compound can obviously inhibit the expansion of cancer cells in lung tissues. The anti-tumor traditional Chinese medicine compound has a wide inhibiting effect on the in vitro migration of tumor cells. The anti-tumor traditional Chinese medicine compound can be clinically used for preventing cancer cell metastasis or relapse after standard treatment of patients with early liver cancer and lung cancer; or as an adjuvant therapy for enhancing and consolidating the efficacy of cancer patients; or as a three-line drug for preventing further spread of cancer in patients with cancer. The anti-tumor traditional Chinese medicine compound can obviously inhibit infiltration and metastasis of hepatocellular carcinoma and non-small cell lung cancer.

Drawings

FIG. 1 is an HPLC chromatogram of an antitumor Chinese medicinal composition and mangiferin standard;

FIG. 2 is a schematic diagram of the antitumor traditional Chinese medicine compound (1g/kg) and mangiferin (50mg/kg) reducing liver tumor growth, wherein A is a schematic diagram of mouse liver cancer in-vivo growth status, B is a schematic diagram of tumor mass at the end of treatment, C is a schematic diagram of mouse weight and tumor mass size during treatment, and D is a schematic diagram of pathological section for treating each group of liver tumors;

FIG. 3 is a schematic diagram of an antitumor Chinese medicinal composition for significantly inhibiting liver cancer lung metastasis, wherein A is a signal schematic diagram of liver tumor cell lung metastasis, and B is a pathological section diagram for treating various groups of lung tissues; 1.2 is two mice in the same group;

FIG. 4 is a schematic diagram of the antineoplastic Chinese medicinal composition significantly inhibiting the expansion of cancer cells in lung tissue, wherein A is a schematic diagram of the in vivo growth of lung tumor in tumor-bearing mice, and B is a schematic diagram of the tumor mass in lung at the end of treatment;

FIG. 5 is a schematic diagram showing that an anti-tumor Chinese medicinal composition has selective inhibition effect on the in vitro growth of tumor cells, wherein A is a schematic diagram showing that BL02 has cytotoxic effect on a liver cancer cell line MHCC97L and PLC/PRF/5, and B is a schematic diagram showing that BL02 has cytotoxic effect on a lung cancer cell line A549;

FIG. 6 is a schematic diagram showing that the anti-tumor Chinese medicinal composition has a broad inhibition effect on the in vitro migration of tumor cells, and the concentration in the diagram shows the drug concentration of BL02, wherein A is a schematic diagram of tumor cells passing through an invasion chamber, and B is a graph of the count of tumor cells passing through the invasion chamber.

Detailed Description

The invention is further illustrated by the following figures and examples, which are not to be construed as limiting the invention.

Experimental medicinal materials and reagents:

the Daphne odora medicinal material is collected from Guizhou province in China.

The safflower gentian is collected from Guizhou province in China.

Hepatoma cell line PLC and lung cancer cell line A549 were purchased from ATCC corporation, and hepatoma cell MHCC97L was obtained from department of surgery of hong Kong university.