阅读说明：本技术 苍术茎叶提取物的应用 (Application of rhizoma atractylodis stem and leaf extract ) 是由 李秀梅 杨培龙 刘婧 杨娟 石冬冬 满晨 王亚冬 潘方方 于 2019-11-01 设计创作，主要内容包括：本发明属于中药加工领域,具体涉及苍术茎叶提取物的应用。本发明的苍术茎叶提取物的应用,包括粉碎苍术茎叶、用溶剂提取、静置后旋转蒸发等步骤。本发明实现了中药资源产业化过程副产物的有效利用,有效延伸资源经济产业链,产生良好的社会经济生态效益。(The invention belongs to the field of traditional Chinese medicine processing, and particularly relates to an application of an atractylodes rhizome stem leaf extract. The application of the atractylodes rhizome stem and leaf extract comprises the steps of crushing atractylodes rhizome stem and leaf, extracting with a solvent, standing, performing rotary evaporation and the like. The invention realizes the effective utilization of the byproduct in the industrialization process of the traditional Chinese medicine resources, effectively extends the economic industrial chain of the resources and generates good social, economic and ecological benefits.)

1. The rhizoma atractylodis stem and leaf extract is used for inhibiting pathogenic bacteria, wherein the pathogenic bacteria are escherichia coli CVCC195, escherichia coli CVCC 1515, salmonella CVCC 3377, salmonella ATCC14028 and/or staphylococcus aureus ATCC 43300.

2. The use as claimed in claim 1, wherein the extract of the stem and leaf of atractylodes lancea is obtained by a method comprising the steps of:

(1) pulverizing stem and leaf of rhizoma Atractylodis to obtain medicinal powder;

(2) adding an extraction solvent into the medicinal powder, and performing ultrasonic extraction, wherein the mass volume ratio of the medicinal powder to the extraction solvent is 1: 40, obtaining an extract by ultrasonic power of 500W, temperature of 50 ℃ and time of 30 min;

(3) standing the extract obtained in the step (2), taking the upper layer solution, centrifuging for 10min at 5000rpm, and taking the supernatant;

(4) and (4) performing rotary evaporation on the supernatant obtained in the step (3), and freeze-drying the supernatant into powder.

3. The use according to claim 2, wherein in step (2), the extraction solvent is water or a 95% ethanol solution.

4. The rhizoma Atractylodis stem and leaf extract can be used for resisting oxidation.

5. The use as claimed in claim 4, wherein the extract of the stem and leaf of Atractylodes lancea is obtained by a method comprising the steps of:

(1) pulverizing stem and leaf of rhizoma Atractylodis to obtain medicinal powder;

(2) adding an extraction solvent into the medicinal powder, and performing ultrasonic extraction, wherein the mass volume ratio of the medicinal powder to the extraction solvent is 1: 40, obtaining an extract by ultrasonic power of 500W, temperature of 50 ℃ and time of 30 min;

(3) standing the extract obtained in the step (2), taking the upper layer solution, centrifuging for 10min at 5000rpm, and taking the supernatant;

(4) and (4) performing rotary evaporation on the supernatant obtained in the step (3), and freeze-drying the supernatant into powder.

6. The use according to claim 5, wherein in step (2), the extraction solvent is water or a 95% ethanol solution.

7. Application of rhizoma Atractylodis stem and leaf water extract in inhibiting inflammatory factors, wherein the inflammatory factors include IL-1 β and/or IL-6.

8. The use as claimed in claim 7, wherein the aqueous extract of stem and leaf of atractylodes lancea is obtained by a method comprising the steps of:

(1) pulverizing stem and leaf of rhizoma Atractylodis to obtain medicinal powder;

(2) adding water into the medicinal powder, and performing ultrasonic extraction, wherein the mass volume ratio of the medicinal powder to the water is 1: 40, obtaining an extract by ultrasonic power of 500W, temperature of 50 ℃ and time of 30 min;

(3) standing the extract obtained in the step (2), taking the upper layer solution, centrifuging for 10min at 5000rpm, and taking the supernatant;

(4) and (4) performing rotary evaporation on the supernatant obtained in the step (3), and freeze-drying the supernatant into powder.

9. The application of the alcohol extract of the stem and leaf of the atractylodes lancea in inhibiting inflammatory factors is disclosed, wherein the inflammatory factors comprise L-6 and/or TNF- α.

10. The use as claimed in claim 9, wherein the alcohol extract of the stem and leaf of atractylodes lancea is obtained by a method comprising the steps of:

(1) pulverizing stem and leaf of rhizoma Atractylodis to obtain medicinal powder;

(2) adding the medicinal powder into a 95% ethanol solution, and performing ultrasonic extraction, wherein the mass volume ratio of the medicinal powder to the 95% ethanol solution is 1: 40, obtaining an extract by ultrasonic power of 500W, temperature of 50 ℃ and time of 30 min;

(3) standing the extract obtained in the step (2), taking the upper layer solution, centrifuging for 10min at 5000rpm, and taking the supernatant;

(4) and (4) performing rotary evaporation on the supernatant obtained in the step (3), and freeze-drying the supernatant into powder.

Technical Field

The invention belongs to the field of traditional Chinese medicine processing, and particularly relates to an application of an atractylodes rhizome stem leaf extract.

Background

Chinese herbal medicine is the material basis and resource guarantee supporting the development of the animal remedy and feed additive industry in our country, it has a long history to be used for prevention and cure of poultry and livestock diseases, in recent years, the industry cluster taking Chinese medicine and natural medicine resources as raw materials develops rapidly, the non-medicinal part and the output of by-products produced in the process of producing medicinal materials and deep processing industrialization are up to hundred million tons, these materials are all not utilized effectively, not only cause the enormous waste of resources, but also cause the serious pollution of the ecological environment.

Atractylodes Lancea (academic name: Atractylodes Lancea (Thunb.) DC.) belongs to Compositae family Atractylodes perennial herb. The rhizome of atractylodes is used as a medicine for activating spleen, is bitter, warm, pungent and strong in property and has the effects of eliminating dampness, eliminating turbidity and relieving pain, and the rhizome of atractylodes contains 5 to 9 percent of volatile oil as a main medicinal component. The non-medicinal parts of the rhizoma atractylodis, such as stems and leaves, are often discarded, and meanwhile, the medicinal components in the stems and leaves of the rhizoma atractylodis are low in content and uncertain in effect, which causes great difficulty in further utilizing the stems and leaves of the rhizoma atractylodis.

Disclosure of Invention

The invention aims to provide application of rhizoma atractylodis stems and leaves, in particular to application in the aspects of inhibiting pathogenic bacteria, resisting oxidation and inhibiting inflammatory factors.

The application of the atractylodes rhizome stem and leaf extract in inhibiting pathogenic bacteria is disclosed, wherein the pathogenic bacteria are escherichia coli CVCC195, escherichia coli CVCC 1515, salmonella CVCC 3377, salmonella ATCC14028 and/or staphylococcus aureus ATCC 43300.

The rhizoma atractylodis stem and leaf extract is applied to antioxidation.

The application of the atractylodes rhizome stem and leaf extract in inhibiting the inflammatory factors is disclosed by the invention, wherein the inflammatory factors comprise IL-1 β and/or IL-6 in an aqueous extract, and the inflammatory factors inhibited by alcohol extraction comprise L-6 and/or TNF- α.

According to the application of the rhizoma atractylodis extract, the rhizoma atractylodis stem and leaf extract is obtained by a method comprising the following steps:

(1) pulverizing stem and leaf of rhizoma Atractylodis to obtain medicinal powder;

(2) adding an extraction solvent into the medicinal powder, and performing ultrasonic extraction, wherein the mass volume ratio of the medicinal powder to the extraction solvent is 1: 40, obtaining an extract by ultrasonic power of 500W, temperature of 50 ℃ and time of 30 min;

(3) standing the extract obtained in the step (2), taking the upper layer solution, centrifuging for 10min at 5000rpm, and taking the supernatant;

(4) and (4) performing rotary evaporation on the supernatant obtained in the step (3), and freeze-drying the supernatant into powder.

According to the application of the rhizoma atractylodis extract, the extraction solvent is water or 95% ethanol solution.

The invention has the beneficial effects that:

the invention extracts the effective components in the stems and leaves of the rhizoma atractylodis to obtain the extract of the stems and leaves of the rhizoma atractylodis, the water/alcohol extract of the stems and leaves of the rhizoma atractylodis has obvious inhibiting effect on pathogenic bacteria such as escherichia coli CVCC195, escherichia coli CVCC 1515, salmonella CVCC 3377, salmonella ATCC14028 and/or staphylococcus aureus ATCC43300, the water/alcohol extract of the stems and leaves of the rhizoma atractylodis has good antioxidation, the water extract of the stems and leaves of the rhizoma atractylodis has good inhibiting effect on inflammatory factors IL-1 β and/or IL-6, and the alcohol extract of the stems and leaves of the rhizoma atractylodis has good inhibiting effect on inflammatory factors L-6 and/or TNF- α.

Detailed Description