阅读说明：本技术 一种利用罗汉果离心废渣制备罗汉果氨基酸的方法 (Method for preparing momordica grosvenori amino acid by using momordica grosvenori centrifugal waste residues ) 是由 黄华学 刘庚贵 刘永胜 于 2019-10-22 设计创作，主要内容包括：本发明涉及一种利用罗汉果离心废渣制备罗汉果氨基酸的方法,包括以下步骤：发酵、水提、离心、膜分离、真空浓缩、结晶洗涤、干燥,其特征在于,所述发酵是三个阶段的覆膜发酵,所述三个阶段分别是酸性发酵、中性发酵和碱性发酵。本发明提供的方法实现了鲜罗汉果的综合利用,在生产主产品罗汉果提取物的同时,利用离心废渣制备了副产品罗汉果氨基酸,变离心废渣为宝,提高了罗汉果的附加值；生产工艺简单,且无需添加生产设备,可以利用罗汉果提取物主产品生产线实现副产品的生产,提高了设备利用率；由于原料是离心废渣,故生产成本低。本专利产品罗汉果氨基酸可用于食品、饮料、保健品领域。(The invention relates to a method for preparing momordica grosvenori amino acid by utilizing momordica grosvenori centrifugal waste residues, which comprises the following steps of: the method is characterized by comprising the following steps of fermentation, water extraction, centrifugation, membrane separation, vacuum concentration, crystallization washing and drying. The method provided by the invention realizes the comprehensive utilization of fresh fructus momordicae, prepares the byproduct fructus momordicae amino acid by using the centrifugal waste residue while producing the main product fructus momordicae extract, changes the centrifugal waste residue into valuable, and improves the additional value of fructus momordicae; the production process is simple, no production equipment is required to be added, the production of byproducts can be realized by utilizing a production line of a main product of the momordica grosvenori extract, and the utilization rate of the equipment is improved; the raw material is centrifugal waste residue, so the production cost is low. The product of the patent, fructus momordicae amino acid, can be used in the fields of food, beverage and health care product.)

1. A method for preparing momordica grosvenori amino acid by utilizing momordica grosvenori centrifugal waste residues comprises the following steps: the method is characterized by comprising the following steps of fermentation, water extraction, centrifugation, membrane separation, vacuum concentration, crystallization washing and drying.

2. The method as claimed in claim 1, wherein the operation of the membrane-covering fermentation is to mix the centrifugal waste residues of momordica grosvenori with warm water at 45-55 ℃, wherein the amount of warm water added is 0.3-0.7 times of the weight of the centrifugal waste residues of momordica grosvenori.

3. The method of claim 1, wherein the acidic fermentation conditions are that the pH of the system is adjusted to be acidic, then food-grade acidic protease and plant extraction enzyme are added, film covering is carried out, and heat preservation fermentation is carried out; and/or the neutral fermentation condition is that the pH value of the system is adjusted to be neutral or alkalescent, food-grade neutral protease is added, film covering is carried out, and heat preservation fermentation is carried out; and/or the alkaline fermentation condition is that the pH of the system is adjusted to be alkaline, food-grade alkaline protease is added, film covering is carried out, and heat preservation fermentation is carried out.

4. The method according to claim 3, wherein the food grade acid protease is added in an amount of 0.5 to 1.0 kg/ton of raw material, and the plant extract enzyme is added in an amount of 0.3 to 0.6 kg/ton of raw material; and/or the addition amount of the food-grade neutral protease is 1.0-3.0 kg/ton of raw material; and/or the addition amount of the food-grade alkaline protease is 1.5-3.5 kg/ton of raw material.

5. The method according to claim 3, wherein the acidic fermentation conditions comprise that the pH of the system is adjusted to be 4.0-5.0, the temperature is kept at 45-55 ℃, and the fermentation time is 3-5 h; and/or the neutral fermentation condition is that the pH value of the system is adjusted to be 7.0-8.5, preferably 7.0-7.5, the heat preservation temperature is 40-50 ℃, and the fermentation time is 4-6 h; and/or the alkaline fermentation conditions are that the pH value of the system is adjusted to be 9.0-10.0, the heat preservation temperature is 45-55 ℃, and the fermentation time is 2-4 h.

6. The method of claim 3, wherein preferably the food grade acid protease specification is APRL type FDY-2205 and the plant extract enzyme specification is SPE-008; and/or the food grade neutral protease is concentrated PDG-2230; and/or the food grade alkaline protease is concentrated PDG-2227.

7. The method as claimed in claim 1, wherein the water extraction is carried out by putting the fermented centrifugal waste residue into an extraction tank, adding water, stirring, extracting, adjusting pH of the solution to weak acidity, extracting at 50-60 deg.C for 1-5 times, preferably 3-4 times, filtering with a filter screen of an extraction system after extraction is finished to obtain an extract; preferably, the adjustment of the pH of the solution to be weakly acidic in the water extraction step means that the solution is adjusted to pH 5.5 to 6.5 with dilute hydrochloric acid.

8. The method of claim 1, wherein the membrane separation is a secondary membrane filtration, and the centrate is passed through an inorganic ceramic membrane system to obtain a yellow, clear, transparent ceramic membrane filtrate; and then, the ceramic membrane filtrate passes through an organic nanofiltration membrane system to obtain a light yellow nanofiltration membrane refining and decoloring solution.

Preferably, the inorganic ceramic membrane is made of alumina or zirconia, and the aperture is 50-500 nm; the organic nanofiltration membrane is made of polyamide, the molecular weight cutoff is 500-1000, and the operating pressure is 2.0-3.0 MPa.

9. The method of claim 1, wherein the crystallization is performed by gradually adding high-concentration ethanol into the vacuum concentrated solution, fully stirring and cooling, gradually precipitating the momordica grosvenori amino acid crystals, and filtering after the crystallization is completed to obtain the momordica grosvenori amino acid crystals; the washing is to add high-concentration ethanol into the grosvenor momordica amino acid crystal, fully stir and stand, gradually dissolve residual pigment, filter, add high-concentration ethanol into the obtained precipitate, and obtain white grosvenor momordica amino acid crystal.

10. The method as claimed in claim 9, wherein in the crystallization process, when the concentration of the ethanol in the system reaches more than 85%, the mixed solvent system is placed at 1-5 ℃ for refrigeration after being fully stirred, so that the crystals are fully precipitated; the high-concentration ethanol is edible ethanol with the ethanol concentration of more than 90%, and the volume-to-weight ratio (kg/L) of the dosage of the high-concentration ethanol added twice in the washing process to the crystalline substance to be washed is 1.5-3: 1 and 0.5-1: 1.

Technical Field

The invention belongs to the technical field of bioengineering and plant extraction and separation, and relates to a method for preparing momordica grosvenori amino acid by using momordica grosvenori centrifugal waste residues. In particular to a method for preparing momordica grosvenori amino acid by using precipitates obtained by centrifuging (including horizontal screw centrifugation and disc centrifugation) extracting solutions (including extracting solution of an extracting tank and extracting solution of a countercurrent extracting tank) of fresh momordica grosvenori or fresh seedless momordica grosvenori in the process of producing momordica grosvenori extract.

Background

Momordica grosvenori (Siraitia grosvenor (Swingle) c. jeffrey), english name: fructumomordica, a fruit of a perennial vine of the cucurbitaceae family. The hybrid fruits are called as the fruits of Lahidrosis, false balsam pear, Momordica grosvenori, Stephania sinica Diels, Japanese cayenne root bark, and nude tortoise, and are known as the fruits of Hydnocarpus, leaf heart shape, male and female variant plants, summer blossom, and autumn harvest. Mainly produced in Longjiang county, Baishou town, Longsheng county, Huanshan county, Huanyuan city, Yongzhou province, Shaoyang province, Huanyuan, etc. The momordica grosvenori has varieties of green peel fruits, changtai fruits, winter melon fruits, Lajiang fruits, rambutan fruits, tung oil tree fruits and the like, and the green peel fruits are planted more. The momordica grosvenori is also one of the first approved medicinal and edible materials in China, and has the main effects of moistening lung to arrest cough, promoting fluid production and reducing phlegm.

The seedless momordica grosvenori is obtained by planting a triploid plant and a diploid male plant of the momordica grosvenori, artificially pollinating a triploid female plant by the diploid male plant during flowering, and bearing fruits on the female plant.

The fructus Siraitiae Grosvenorii and Seedless fructus Siraitiae Grosvenorii contain abundant flavor and nutritional components, and triterpene saponin non-sugar sweet component.

Chemical composition

The momordica grosvenori and the seedless momordica grosvenori both contain momordica grosvenori triterpenoid saponin, sucrose and glucose (fructose), the content of fructose (fructose) is 14%, more than ten kinds of amino acids, proteins, fatty acids, flavonoid compounds, vitamin C, 26 inorganic elements such as manganese, iron, nickel, selenium, tin, iodine, molybdenum and the like, and trace elements.

1. Cucurbitacine triterpenes are main effective components in fructus momordicae, and have a common aglycone structure. The substances in the grosvenor momordica are sweet substances or slightly sweet substances except a few substances. Wherein, the mogroside V is the main sweet component, has no toxicity, low calorie, high sweetness and good heat stability, and is one of the substances developed as natural sweeteners.

2. The protein and the amino acid momordica grosvenori dried fruits contain 7.1 to 7.8 percent of protein. In the hydrolysate, 18 kinds of amino acids were completed except tryptophan which was not measured. Among the amino acids tested, the higher content is glutamic acid (108.2-113.3mg/kg), aspartic acid (93.9-112.5mg/kg), valine (52.5-55.5mg/kg), alanine (49.9-66.8mg/kg), and leucine (48.5-56.7 mg/kg). Glutamic acid is the most necessary nonessential amino acid in human body and is an important raw material for synthesizing glutathione, so that the momordica grosvenori has high nutritional value.

3. The fructus Siraitiae Grosvenorii contains 24 inorganic elements, including 16 trace elements and broad elements essential to human body, and potassium (12290mg/kg), calcium (667mg/kg), and magnesium (550 mg/kg). The selenium contained in the fructus momordicae reaches 0.186mg/kg, which is 2-4 times of that of the grain, and the element selenium has better curative effect on preventing and treating coronary heart disease, resisting aging and resisting cancer.

Second, present State of research

At present, many researches on momordica grosvenori and seedless momordica grosvenori are carried out in China, the researches comprise chemical components of momordica grosvenori, an extraction and separation process, momordica grosvenori extract (mogroside V) and application thereof, momordica grosvenori concentrated juice, production wastewater treatment, momordica grosvenori related products and the like, but the researches on precipitates, namely centrifugal waste residues obtained by centrifuging extracting solutions (including extracting solution of an extracting tank and extracting solution of a countercurrent extracting tank) of fresh momordica grosvenori or fresh seedless momordica grosvenori in the process of producing the momordica grosvenori extract are less. At present, some manufacturers for producing the momordica grosvenori extract at home dry the centrifugal waste residues in the production process of fresh momordica grosvenori to serve as feed additives for sale; one part is dried in the sun and used as boiler fuel; and a part of the waste which cannot be treated in time is discharged as solid waste. Although the treatment modes are simple, the resource waste of the centrifugal waste residues of the momordica grosvenori with certain economic value is caused, the comprehensive utilization of the centrifugal waste residues of the momordica grosvenori cannot be realized, the additional value of byproducts of the momordica grosvenori extract cannot be improved, or certain pollution and damage to the environment are caused. The main reason is that production equipment and processing capacity of each production enterprise are different, the centrifugal waste residue processing level is different due to different possessed technologies, or a supply chain of terminal application without a fructus momordicae extract byproduct and the like all cause waste of centrifugal waste residue resources in the fructus momordicae production process with a certain added value.

The centrifugal waste residue in the production process of the momordica grosvenori mainly contains components such as protein, plant fiber, pectin, mucilage, pigment and the like, and the momordica grosvenori amino acid can be prepared by effectively utilizing the centrifugal waste residue in the production process of the fresh momordica grosvenori through the self-owned patent technology and combining the production process of a main product momordica grosvenori extract, so that the momordica grosvenori amino acid with certain economic value is obtained, the comprehensive utilization of the momordica grosvenori is realized, the wastes are changed into valuable, and obvious economic and social.

The invention patent in China: the patent with the application numbers of CN201811158344.X, CN201810508219.0, CN201711034468.2 and CN201010107948.9 relates to protein and amino acid in waste liquid of momordica grosvenori leaf and momordica grosvenori production.

Detailed description of the domestic invention patents:

1. patent application No. cn201811158344.x discloses a method for extracting tea polyphenol, total amino acids and flavone compounds from momordica grosvenori leaves, which comprises the following steps: (1) percolation extraction: pulverizing dry leaves of fructus Siraitiae Grosvenorii, placing into a percolator, compacting, adding water, percolating at room temperature to obtain percolate; (2) and (3) separating tea polyphenol: adding organic solvent, extracting under stirring at room temperature, standing for layering, washing the separated organic layer with acid water solution to colorless, concentrating, drying, and recrystallizing to obtain tea polyphenols. The invention also discloses a method for simultaneously extracting the total amino acid and the flavone compound. The method takes the momordica grosvenori leaves as raw materials to extract amino acid, corresponding literature reports are few, and the low content of the raw materials directly causes low product yield and difficult separation and refining, and has no industrial production value and application prospect.

2. Patent application No. CN201810508219.0 discloses a method for separating water-soluble dietary fiber and collagen from waste liquid of fructus momordicae, which comprises the following steps: (1) adjusting the pH value of the waste liquid of the momordica grosvenori production to be neutral, adding a protease preparation, heating, preserving heat and carrying out enzymolysis to obtain an enzymolysis liquid; (2) filtering with ultrafiltration membrane, and collecting the filtrate; (3) performing chromatography with Sephadex column chromatography, collecting eluate, concentrating under reduced pressure, and drying to obtain water soluble dietary fiber; (4) eluting the Sephadex chromatographic column after collecting the effluent with an eluant, filtering the eluate with a nanofiltration membrane, collecting the nanofiltration membrane retentate, concentrating under reduced pressure, and freeze-drying to obtain the nanometer collagen. The target product prepared by the method is collagen instead of momordica grosvenori amino acid; the used sephadex is expensive, and the amount of the processed sample is small, which directly causes the over-high production cost and whether the sephadex has industrial production value or not, and needs further determination; the water-soluble dietary fibers and the collagen are both macromolecular substances, and the waste liquid of the momordica grosvenori contains a large amount of pigments, so whether a qualified target product can be obtained or not through the process, and how the purity of the target product is, the scientificity needs to be researched.

3. Patent application No. CN201711034468.2 discloses a method for producing momordica grosvenori protein from the waste liquid of momordica grosvenori glycoside extraction. The patent method produces the momordica grosvenori protein powder from the waste liquid discharged from the momordica grosvenori glycoside adsorption resin column, and fully improves the conversion rate of total protein and the conversion rate of momordica grosvenori protein. The raw material of the method is the mogroside extraction waste liquid of the momordica grosvenori, the target product is momordica grosvenori protein, but the raw material is obtained by heating for a certain time in the production process of momordica grosvenori extract, and the protein is denatured by heating, so that the water solubility is poor directly; the ultrafiltration membrane permeate collected by the method is limited in molecular weight range, is subjected to protein adsorption resin chromatography, is used for desorption solvent, is no other purification step, is the target product protein obtained contain other impurities and pigments, and is the purity of the target product? And are yet to be further studied.

4, patent No. CN201010107948.9, discloses a method for extracting multiple active ingredients from dried fructus Siraitiae Grosvenorii, wherein the method relates to a process for preparing polysaccharide, oligosaccharide and amino acid by hydrolyzing waste residue with gastric enzyme, the method is not a process for preparing amino acid from centrifugal waste residue of fructus Siraitiae Grosvenorii, the utilization rate of protein/amino acid is not high, the enzymolysis of gastric enzyme is not sufficient, the yield of amino acid is low,

in summary, there is currently a lack of a method for efficiently producing luo han guo amino acids, particularly from luo han guo centrifuge residues.

Disclosure of Invention

The invention overcomes the defects in the prior art, takes the centrifugal waste residue of fresh momordica grosvenori or fresh seedless momordica grosvenori in the process of producing the momordica grosvenori extract as the raw material, produces and prepares momordica grosvenori amino acid with certain economic value in a large scale, realizes the comprehensive utilization of momordica grosvenori, changes waste into valuable and creates obvious economic and social benefits; a simple and feasible process route is found, the production cost is reduced, the working efficiency is improved, and the mass production is realized.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a method for preparing momordica grosvenori amino acid by utilizing momordica grosvenori centrifugal waste residues comprises the following steps: the method is characterized by comprising the following steps of fermentation, water extraction, centrifugation, membrane separation, vacuum concentration, crystallization washing and drying.

The centrifugal waste residue of fructus Siraitiae Grosvenorii is prepared by centrifuging (including horizontal screw centrifugation and disc centrifugation) extractive solution (including extractive solution of extraction tank and extractive solution of countercurrent extraction tank) of fresh fructus Siraitiae Grosvenorii or fresh fructus Siraitiae Grosvenorii in fructus Siraitiae Grosvenorii extract production process to obtain precipitate, i.e. centrifugal waste residue in fresh fructus Siraitiae Grosvenorii production process.

The film-covered fermentation refers to the film-covered heat-preservation fermentation of samples by adding water and proteases with different specifications and models in a wet solid state, the film-covered heat-preservation fermentation is different from the liquid state protease-added fermentation in a general solution system, and the film-covered fermentation has the advantages that: firstly, the raw material, namely the centrifugal waste residue of the momordica grosvenori is in a water-insoluble solid state, and although the centrifugal waste residue contains higher protein content, the efficiency of solution fermentation is not high, and a large amount of reagents are needed, so that the cost is increased, and the centrifugal waste residue is not green and environment-friendly; secondly, the film-covered fermentation has the remarkable advantages of high efficiency, good effect, simple and convenient operation and no occupation of production equipment, and the external fermentation tank is used for realizing high-efficiency fermentation; and finally, the film-covered fermentation process is easy to control and safe to operate.

The operation of the film-covering fermentation is that firstly the centrifugal waste residue of the grosvenor momordica fruit is evenly mixed with warm water, the film-covering fermentation is carried out by 3 stages, and each stage adopts different enzymes and different conditions.

The temperature of the warm water is 45-55 ℃, and the adding amount of the warm water is 0.3-0.7 time of the weight of the centrifugal waste residue of the momordica grosvenori.

In the preferable technical scheme of the invention, the acidic fermentation condition is that the pH of the system is adjusted to be acidic, then food-grade acidic protease and plant extraction enzyme are added, and the film is covered, and the fermentation is carried out under the condition of heat preservation; the neutral fermentation condition is that the pH value of the system is adjusted to be neutral or alkalescent, food-grade neutral protease is added, film covering is carried out, and heat preservation fermentation is carried out; and the alkaline fermentation condition is to adjust the pH of the system to be alkaline, add food-grade alkaline protease, cover the film and perform heat preservation fermentation.

Preferably, the specification model of the food-grade acid protease is APRL type FDY-2205, mainly acts on an amido bond, can cut peptide bonds formed by various amino acids, and preferentially cuts peptide bonds between aromatic or hydrophobic amino acid residues at two ends; the plant extraction enzyme is SPE-008, the main enzyme system is protease, and the compound enzyme system such as cellulase is used, so that protein can be hydrolyzed into amino acid, turbidity of solution can be reduced, viscosity of an extracting solution is reduced, filtering speed is increased, the phenomena of layered flocculation and the like of precipitates are effectively solved, a good clarifying effect is achieved, and the optimal pH of the 2 enzyme action systems is 4-5 and basically accords with the pH of raw materials.

More preferably, the addition amount of the food-grade acid protease is 0.5-1.0 kg/ton of raw materials, and the dosage of the plant extraction enzyme is 0.3-0.6 kg/ton of raw materials.

Further preferably, the acidic fermentation condition is that the pH value of the system is adjusted to be 4.0-5.0, the heat preservation temperature is 45-55 ℃, and the fermentation time is 3-5 h.

Preferably, the food grade neutral protease specification model is high-activity concentrated PDG-2230, mainly contains endopeptidase activity and has small amounts of aminopeptidase and carboxypeptidase activity, the aminopeptidase and the carboxypeptidase hydrolyze peptide bonds closest to the N end or the C end of a protein substrate, the acting point of the endopeptidase is positioned in the protein and far away from the N end and the C end of the protein substrate, and the enzymes act together to realize good enzymolysis conversion; the neutral protease has a wider pH action range and has good activity under neutral and alkaline conditions, so that the neutral protease is used as a transition enzyme between acidity and alkalinity to realize seamless connection and ensure the effect of enzymolysis conversion.

More preferably, the addition amount of the food grade neutral protease is 1.0-3.0 kg/ton raw material.

Further preferably, the neutral fermentation condition is that the pH value of the system is adjusted to be 7.0-8.5, preferably 7.0-7.5, the heat preservation temperature is 40-50 ℃, and the fermentation time is 4-6 h.

Preferably, the specification model of the food-grade alkaline protease is high-activity concentrated PDG-2227, the enzyme activity center contains serine, the protease belongs to serine endoprotease, the protease has strong capability of decomposing protein, and the protein which is not subjected to enzymolysis by acidic protease and neutral protease can be basically converted into water-soluble amino acid.

More preferably, the addition amount of the food-grade alkaline protease is 1.5-3.5 kg/ton of raw materials.

Further preferably, the alkaline fermentation conditions are that the pH value of the system is adjusted to 9.0-10.0, the heat preservation temperature is 45-55 ℃, and the fermentation time is 2-4 h.

The water-insoluble protein is basically and completely converted into the water-soluble amino acid through the synergistic action of the different proteases. Amino acid is a special substance, has amino and carboxyl groups in the molecular structure, has amphipathy, and is easy to damage the molecular structure of the amino acid if the extraction condition is not well controlled. Under the conditions of pH and temperature, the amino acid molecular structure is protected from being damaged, and the fermented amino acid can be basically and completely extracted and is easy to separate from impurities. If the extraction is carried out according to the conventional method, the final extraction product is only about 65 percent of the method because part of amino acids are damaged.

The water extraction is carried out by putting fermented centrifugal waste residue into extraction tank, adding water, stirring, extracting, adjusting pH of solution to weak acidity, extracting at 50-60 deg.C for 1-5 times, preferably 3-4 times, and filtering with filter screen of extraction system to obtain extractive solution.

Preferably, the adjustment of the pH of the solution to be weakly acidic in the water extraction step means that the solution is adjusted to pH 5.5 to 6.5 with dilute hydrochloric acid.

The centrifugation is to sequentially pass the extracting solution through a horizontal screw centrifuge and a disc centrifuge and collect the centrifugate;

the membrane separation is secondary membrane filtration, and the centrifugate passes through an inorganic ceramic membrane system to obtain clear and transparent ceramic membrane filtrate; and then, the ceramic membrane filtrate passes through an organic nanofiltration membrane system to obtain a light yellow nanofiltration membrane refining and decoloring solution.

Preferably, the inorganic ceramic membrane is made of alumina or zirconia, and the aperture is 50-500 nm; the organic nanofiltration membrane is made of polyamide, the molecular weight cutoff is 500-1000, and the operating pressure is 2.0-3.0 MPa.

And the vacuum concentration is to carry out vacuum concentration on the nanofiltration membrane refined and decolorized solution to a certain solid content to obtain a vacuum concentrated solution.

Preferably, vacuum concentration is at a vacuum of 0.01-0.1MPa to a solids content of greater than 40 wt%.

The crystal washing is to add a certain amount of ethanol, preferably edible ethanol, into the vacuum concentrated solution, fully stir and cool the solution to gradually separate out the momordica grosvenori amino acid crystals, and filter the solution after the crystals are completely crystallized to obtain the momordica grosvenori amino acid crystals; adding high-concentration ethanol into the fructus Siraitiae Grosvenorii amino acid crystal, stirring and standing, dissolving residual pigment gradually, filtering, and washing the obtained precipitate with high-concentration ethanol to obtain white fructus Siraitiae Grosvenorii amino acid crystal.

Preferably, in the crystallization process, high-concentration ethanol is gradually added into the concentrated solution, the concentration of the ethanol in the system is gradually increased, and when the concentration of the ethanol in the system reaches 85%, the mixed solvent system is placed at 1-5 ℃ for refrigeration after being fully stirred, so that crystals are fully precipitated. The filtration comprises suction filtration and cloth bag centrifugation, and the solvent is required to gradually permeate through the solid layer. The volume-to-weight ratio (kg/L) of the dosage of the high-concentration ethanol added twice in the washing process to the crystalline substance to be washed is 1.5-3: 1 and 0.5-1: 1.

the high-concentration ethanol is preferably edible ethanol, and the concentration of the ethanol is more than 90 wt%.

The drying is to dry the grosvenor momordica amino acid crystal by air blast or vacuum, the drying temperature is 55-65 ℃, and the drying degree is that the moisture is less than 5 percent, thus obtaining the grosvenor momordica amino acid product.

The principle of the invention is that the momordica grosvenori amino acid with certain economic value is prepared on a large scale by taking centrifugal waste residue in the production process of fresh momordica grosvenori as a raw material, wherein the centrifugal waste residue is obtained by centrifuging extract (comprising extract of an extraction tank and extract of a countercurrent extraction tank) of fresh momordica grosvenori or fresh momordica grosvenori without seeds in the production process of momordica grosvenori extract.

The raw materials mainly contain components such as protein, plant fiber, pectin, mucilaginous substances, pigment and the like, wherein the original protein with better water solubility is changed into a water-insoluble denatured protein component through operations such as heating and the like in the extraction process, thereby reducing the extraction efficiency of the protein. The inventor of the invention unexpectedly discovers that the specific enzyme combination is used to act on different structural parts of protein molecules, the enzymolysis is carried out under the optimal conditions, the reaction is mild and thorough, and the macromolecular protein is completely hydrolyzed into micromolecular water-soluble amino acid; controlling extraction conditions, protecting the converted amino acid from being damaged, realizing complete extraction, and separating the water-soluble momordica grosvenori amino acid from other water-insoluble impurities such as plant fibers, partial pectin, mucilaginous substances and the like through a filtering system and a centrifugal process of extraction equipment; then, through an integrated membrane system, wherein the cross-flow filtration clarification technology of an inorganic ceramic membrane system realizes the clarification of the centrifugate, completely removes the influence of fine mechanical impurities, namely water-insoluble components, on the product quality, and can ensure the clarification of the final product, namely the dissolved solution of amino acid; the molecular weight interception technology of the organic nanofiltration membrane system realizes the separation of macromolecular impurities, such as part of pectin, mucilaginous substances and other components, incompletely enzymolyzed protein, most of pigments and other substances from the target product, namely the momordica grosvenori amino acid, and has good effect and high efficiency; refining a nanofiltration membrane and concentrating a decolorized solution to a certain solid content through vacuum concentration so as to be beneficial to later-stage crystallization; decolorizing and crystallizing by using a mixed solvent system, and washing, namely adding high-concentration edible ethanol into a vacuum concentrated solution with high solid content to crystallize and separate out amino acid, dissolving pigment in a mother solution, and further removing residual pigment by washing to obtain a grosvenor momordica amino acid product with high purity and good color; drying to obtain the finished product of the momordica grosvenori amino acid.

The invention has the following beneficial effects:

(1) the momordica grosvenori amino acid is prepared by taking centrifugal waste residues in the production process of fresh momordica grosvenori as raw materials. The obtained product is completely from the momordica grosvenori or the momordica grosvenori without seeds, only purified water and edible ethanol are used in the production process, no other additives are added, and the momordica grosvenori beverage is safe to eat and has high nutritional value.

(2) The comprehensive utilization of the fresh momordica grosvenori is realized, waste is changed into valuable, centrifugal waste residues in the production process of the fresh momordica grosvenori are fully utilized, the momordica grosvenori extract serving as a main product is produced, meanwhile, the momordica grosvenori amino acid serving as a byproduct with economic value is prepared, and obvious economic and social benefits are created.

(3) Different types of enzymes are used to act on different structural parts of protein molecules, enzymolysis is carried out under the optimal condition, the reaction is mild and thorough, macromolecular protein is completely hydrolyzed into micromolecular water-soluble amino acid, severe hydrolysis conditions such as acid hydrolysis or alkali hydrolysis which can destroy the amino acid are not adopted, protease is not simply added for enzymolysis, and the quality of the product cannot be effectively and stably controlled by enzymolysis in solution.

(4) The invention screens proper extraction conditions, realizes complete extraction while protecting the converted amino acid from being damaged, and improves the yield of amino acid products; the clarification of the centrifugate is realized by using an integrated membrane system and a cross-flow filtering clarification technology of an inorganic ceramic membrane system, the influence of fine mechanical impurities on the product quality is completely removed, and the clarification of the final product amino acid solution can be ensured; the molecular weight interception technology of the organic nanofiltration membrane system realizes the separation of macromolecular impurities, incompletely enzymolyzed proteins, most pigments and other substances from the target product amino acid, and has good effect and high efficiency; and (3) decoloring, crystallizing and washing by using a mixed solvent system, further removing residual pigment, and obtaining the momordica grosvenori amino acid product with high purity and good color, wherein only edible ethanol and purified water are used, a complex decoloring and crystallizing technology is not used, and an organic solvent harmful to a human body is not used, so that the edibility and the safety of the product are improved.

(5) The problem of influence of centrifugal waste residues in the production process of the fresh momordica grosvenori on the environment is solved by an innovative method, and the problem of solid waste of the fresh momordica grosvenori is fundamentally solved.

(6) Establishes a novel treatment method of fresh momordica grosvenori centrifugal waste residue, and has simple and feasible production process, low production cost, simple equipment and low treatment cost, thus being suitable for popularization.

Detailed Description

The present invention will be further described with reference to the following examples.

The raw material used in the embodiment of the invention, namely the centrifugal waste residue in the production process of the fresh momordica grosvenori comes from the production workshop of the company. The purified water used in the embodiment of the invention is prepared by pure water equipment used in a production workshop of the company. The raw and auxiliary materials used in the examples of the present invention are commercially available in a conventional manner unless otherwise specified.

In the embodiment of the invention, the amino acid content of the momordica grosvenori is determined by a ninhydrin colorimetric method according to the national standard GB/T5009.124-2003 determination of amino acids in food.