阅读说明：本技术 一种多糖、硫酸化多糖和在美白淡斑化妆品中的应用 (Polysaccharide, sulfated polysaccharide and application in whitening and spot-lightening cosmetics ) 是由 陈敏 于 2019-10-26 设计创作，主要内容包括：本发明公开了一种多糖、硫酸化多糖和在美白淡斑化妆品中的应用。本发明从金钱蒲全草中制备得到了一种多糖含量在95％左右的金钱蒲多糖,并对其衍生化修饰得到一种硫酸化金钱蒲多糖。酪氨酸酶抑制活性测试结果表明,金钱蒲多糖对酪氨酸酶具有较高的抑制作用,将金钱蒲多糖硫酸化修饰得到的硫酸化金钱蒲多糖对酪氨酸酶的抑制作用更强,抑制强度提高了80％以上,金钱蒲多糖及其衍生化产物硫酸化金钱蒲多糖都具有用作化妆品添加剂提高化妆品美白淡斑效果的前景。(The invention discloses a polysaccharide, a sulfated polysaccharide and application in whitening and spot-lightening cosmetics. The invention prepares a desmodium polysaccharide with the polysaccharide content of about 95 percent from the whole herb of desmodium, and obtains a sulfated desmodium polysaccharide by derivatization and modification of the desmodium polysaccharide. The tyrosinase inhibitory activity test result shows that the zamioculcas zamiifolia polysaccharide has a higher inhibitory effect on tyrosinase, the sulfated zamioculcas zamiifolia polysaccharide obtained by sulfating and modifying the zamioculcas zamiifolia polysaccharide has a stronger inhibitory effect on tyrosinase, the inhibitory strength is improved by more than 80%, and the zamioculcas zamiifolia polysaccharide and the sulfated zamioculcas zamiifolia polysaccharide serving as a derivative thereof have the prospect of being used as a cosmetic additive to improve the whitening and spot-lightening effects of cosmetics.)

1. A polysaccharide, characterized by being prepared by the following method:

taking cleaned and dried whole cattail pollen, crushing, sieving, adding deionized water for heating and extracting, filtering an extracting solution, concentrating under reduced pressure, centrifuging, carrying out alcohol precipitation on a supernatant by using 4 times of volume of anhydrous ethanol, redissolving a precipitate by using the deionized water to the original supernatant volume, repeatedly carrying out alcohol precipitation once by using 4 times of volume of anhydrous ethanol, sequentially washing the precipitate by using ethanol and acetone alternately, volatilizing a solvent, redissolving the precipitate in the deionized water, deproteinizing by using a Sevag method, and carrying out freeze drying to obtain the cattail pollen polysaccharide.

2. The polysaccharide of claim 1, wherein: pulverizing, and sieving with 40 mesh sieve.

3. The polysaccharide of claim 1, wherein: deionized water is added according to the feed-liquid ratio of 1kg to 8-12L.

4. The polysaccharide of claim 1, wherein: the temperature for heating and extracting is 75-85 ℃.

5. The polysaccharide of claim 1, wherein: concentrating 1L concentrate under reduced pressure according to 1kg whole plant.

6. The polysaccharide of claim 1, wherein: the centrifugation condition is 4000r/min for 10 min.

7. The polysaccharide of claim 1, wherein: the precipitate was washed 3 times with ethanol and acetone in sequence, in an alternating manner, according to 1g of precipitate and 50mL of solvent.

8. Use of the polysaccharide according to any one of claims 1 to 7 for preparing a whitening and spot-lightening cosmetic.

9. A sulphated product of a polysaccharide as claimed in any one of claims 1 to 7.

10. Use of the sulphated product of claim 9 for the preparation of a cosmetic product for lightening skin spots.

Technical Field

The invention belongs to the field of chemistry, and particularly relates to a polysaccharide, a sulfated polysaccharide and application of the polysaccharide and the sulfated polysaccharide in whitening and spot-lightening cosmetics.

Background

Tyrosinase inhibitors are currently recognized whitening additives for whitening cosmetics, such as the whitening spot-lightening ingredient kojic acid. Therefore, the strengthening of the development of tyrosinase inhibitors can enrich the variety of whitening and spot-fading additives in cosmetics.

The plant polysaccharide is a gift for human beings, and many plant polysaccharides have biological activities, such as health promotion effects of regulating immunity, resisting tumor, reducing blood sugar, reducing blood lipid, resisting radiation, resisting bacteria and virus, and protecting liver. Therefore, the plant polysaccharide has been widely applied to the fields of medical science, catering and other public lives. The application of the plant polysaccharide in cosmetics has the advantages of safety and no irritation.

The Longhairy antenoron herb Acorusgramineus Soland is a plant of calamus of Araceae, originates from the shade and wet part at the ditch edge, has intense fragrance by being torn into veins, and is mainly distributed in southern provinces of Yangtze river basin. The lysimachia christinae hance is commonly used for treating epilepsy, phlegm syncope, fever, coma, amnesia, qi block, deafness, chest distress, stomachache, abdominal pain, wind-cold-dampness arthralgia, carbuncle, heat toxin, traumatic injury and other diseases in folk, and is mainly treated by rhizome. Research shows that the desmodium whole plant contains various chemical components, such as flavone, saponin, polysaccharide, etc.

The research on the polysaccharide in the whole herb of longhairy antenoron is very little, and no activity report on the tyrosinase inhibition effect exists.

Disclosure of Invention

The invention aims to provide a polysaccharide, a sulfated polysaccharide and application in whitening and spot-lightening cosmetics.

The purpose of the invention is realized by the following technical scheme:

a polysaccharide prepared by the method comprising:

cleaning and drying herba Lysimachiae Christinae whole plant, pulverizing, sieving, adding deionized water, heating and extracting, filtering the extractive solution, concentrating under reduced pressure, centrifuging, precipitating the supernatant with 4 times volume of anhydrous ethanol, redissolving the precipitate with deionized water to the original supernatant volume, repeatedly precipitating with 4 times volume of anhydrous ethanol once, washing the precipitate with ethanol and acetone alternately, volatilizing solvent, redissolving in deionized water, deproteinizing by Sevag method, and freeze drying to obtain herba Lysimachiae Christinae polysaccharide

Preferably, the powder is passed through a 40 mesh screen after grinding.

Preferably, deionized water is added according to the feed-liquid ratio of 1kg to 8-12L.

Preferably, the temperature for heating and extracting is 75-85 ℃.

Preferably, 1L of the concentrated solution is concentrated under reduced pressure according to 1kg of whole plant.

Preferably, the centrifugation conditions are 4000r/min for 10 min.

Preferably, the precipitate is washed 3 times alternately with ethanol, acetone, and then 1g of precipitate versus 50mL of solvent.

The polysaccharide is used for preparing whitening and spot-fading cosmetics.

A sulphated product of the above polysaccharide.

The use of the above sulphated product for the preparation of a cosmetic product for lightening skin spots.

Has the advantages that:

the invention prepares a desmodium polysaccharide with the polysaccharide content of about 95 percent from the whole herb of desmodium, and obtains a sulfated desmodium polysaccharide by derivatization and modification of the desmodium polysaccharide. The tyrosinase inhibitory activity test result shows that the zamioculcas zamiifolia polysaccharide has a higher inhibitory effect on tyrosinase, the sulfated zamioculcas zamiifolia polysaccharide obtained by sulfating and modifying the zamioculcas zamiifolia polysaccharide has a stronger inhibitory effect on tyrosinase, the inhibitory strength is improved by more than 80%, and the zamioculcas zamiifolia polysaccharide and the sulfated zamioculcas zamiifolia polysaccharide serving as a derivative thereof have the prospect of being used as a cosmetic additive to improve the whitening and spot-lightening effects of cosmetics.

Drawings

FIG. 1 is the scanning electron microscope image of the Gentiana pulmonanthus polysaccharide (A) and sulfated Gentiana pulmonanthus polysaccharide (B) prepared by the method of example 1.

FIG. 2 is the IC50 values for inhibition of tyrosinase by Lysimachia christinae Hance polysaccharide, sulfated Lysimachia christinae Hance polysaccharide and the positive drug kojic acid.

Detailed Description

The following detailed description of the present invention is provided in connection with the accompanying drawings and examples, but not intended to limit the scope of the invention.