阅读说明：本技术 一种南极磷虾虾溶浆的制备方法 (Preparation method of euphausia superba soluble pulp ) 是由 曲有乐 欧阳小琨 卢雨清 杨立业 郁迪 于 2019-10-10 设计创作，主要内容包括：本发明涉及水产品制备技术领域,为解决传统虾溶浆利用率低、直接排放导致环境污染、资源浪费的问题,提供了一种南极磷虾虾溶浆的制备方法,包括以下步骤:(1)将南极磷虾绞碎制成肉糜状,加入水,搅拌得匀浆液；(2)添加复合蛋白酶,酶解,得第一酶解液；(3)添加风味蛋白酶,酶解,得第二酶解液；(4)震荡,高速离心,所得上清液即为南极磷虾虾溶浆。本发明采用分步水解法,木瓜蛋白酶有助于南极磷虾中虾壳和虾肉成分的酶解；风味蛋白酶可以去除苦味,改善口感,并且提高虾溶浆中氨基酸的含量。(The invention relates to the technical field of aquatic product preparation, and provides a preparation method of euphausia superba dissolving pulp, aiming at solving the problems of low utilization rate of the traditional shrimp dissolving pulp, environmental pollution caused by direct discharge and resource waste, wherein the preparation method comprises the following steps of (1) mincing euphausia superba into meat paste, adding water, and stirring to obtain homogenate; (2) adding compound protease, and performing enzymolysis to obtain a first enzymolysis liquid; (3) adding flavourzyme, and carrying out enzymolysis to obtain a second enzymolysis liquid; (4) and oscillating and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp. The invention adopts a stepwise hydrolysis method, and the papain is beneficial to the enzymolysis of the components of the shrimp shell and the shrimp meat in the antarctic krill; the flavourzyme can remove bitter taste, improve mouthfeel and increase the content of amino acid in the shrimp dissolving pulp.)

1. A preparation method of antarctic krill shrimp soluble pulp is characterized by comprising the following steps:

(1) mincing Antarctic krill into minced meat, adding water with the weight being 2-4 times that of the minced meat, and uniformly stirring to obtain homogenate;

(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid;

(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid;

(4) and oscillating the second enzymolysis liquid under the water bath condition, and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp.

2. The preparation method of the euphausia superba soluble pulp according to claim 1, wherein the compound protease is composed of the following components in parts by weight: 10-20 parts of papain and 30-50 parts of trypsin.

3. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (2), the addition amount of the compound protease is 0.3-0.8% of the weight of the euphausia superba.

4. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (3), the addition amount of the flavourzyme is 2-3% of the weight of the euphausia superba.

5. The method for preparing the euphausia superba soluble pulp according to claim 1, wherein in the step (2), the pH value during the enzymolysis is controlled to be 8-9, and the temperature is controlled to be 45-50 ℃.

6. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (3), the pH value is controlled to 6-7 and the temperature is controlled to 25-35 ℃ during the enzymolysis process.

7. The preparation method of the Antarctic krill shrimp soluble pulp according to claim 1, wherein in the step (4), the water bath temperature is controlled to be 37-40 ℃, and the rotation speed of the high-speed centrifugation is controlled to be 18000-20000 r/min.

Technical Field

The invention relates to the technical field of aquatic product preparation, and particularly relates to a preparation method of euphausia superba soluble pulp.

Background

The aquaculture industry develops at a high speed, and the shrimp meal yield cannot be synchronized, so that the demand of shrimp meal resources is short, and therefore, how to improve the utilization efficiency of the marine caught shrimps as feed products and reduce the use amount of the shrimp meal in artificial compound feed is an important research topic.

The antarctic krill shrimp soluble paste is a shrimp byproduct in the production process of shrimp meal, the components in the shrimp soluble paste are complex, a large amount of micro granular substances suspended in water, such as protein, peptide, amino acid and the like dissolved in water, biological amine, trimethylamine oxide, a small amount of oil components, trace elements and the like are also contained, and the biological active components have irreplaceable effects on the nutritional value of feed. However, most enterprises generally discharge the waste water directly, and the nutrient components in the waste water react with microorganisms and algae to cause malodor and red tide, so that the environment is polluted and the resource is wasted. For the above reasons, how to prepare a treated safe shrimp slurry from Antarctic krill has become an important research topic.

Chinese patent literature discloses a krill meal feed and a production process, and the publication number is CN 108850681A, the feed takes Antarctic krill meal as a raw material, has rich and balanced nutrition and good palatability, can provide sufficient nutrition for fish growth, is beneficial to improving the culture yield, and has low cost, convenient preparation and good economic benefit. However, the shrimp by-product Antarctic krill shrimp soluble pulp in the production process of the shrimp meal is not fully utilized, which causes resource waste.

Disclosure of Invention

The invention provides a preparation method of euphausia superba dissolving pulp with high safety and high resource utilization rate, aiming at overcoming the problems of low utilization rate of the traditional shrimp dissolving pulp, environmental pollution caused by direct discharge and resource waste.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of antarctic krill shrimp soluble pulp comprises the following steps:

(1) mincing Antarctic krill into minced meat, adding water with the weight being 2-4 times that of the minced meat, and uniformly stirring to obtain homogenate;

(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid;

(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid;

(4) and oscillating the second enzymolysis liquid under the water bath condition, and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp.

The hydrolysis of the shrimp dissolving pulp comprises an acid hydrolysis method, an alkali hydrolysis method, an enzymolysis method and the like, wherein the enzymolysis method destroys a peptide chain structure of protein in the shrimp dissolving pulp through protease to dissociate the protein into amino acid, the reaction condition is mild, the energy consumption can be effectively reduced, the cost is lower, and the waste liquid discharge is less, so the method is an ideal choice for treating the shrimp dissolving pulp. The invention can hydrolyze macromolecules such as protein, polypeptide and the like in the euphausia superba into amino acid which is easy to be absorbed by organisms by carrying out enzymolysis treatment on shrimp dissolving pulp which is a shrimp byproduct of the euphausia superba, so that the protein, the polypeptide and the like become raw materials suitable for storage and industrial development, the utilization rate of natural protein is improved, the resource waste is reduced, the discharge of the shrimp dissolving pulp is reduced, and the invention plays a great role in protecting the environment. The obtained hydrolysate contains free amino acids, and can be used as raw material for industrial production of liquid protein fertilizer. And the obtained hydrolysate can be subjected to deodorization treatment, so that the uncomfortable influence of the hydrolysate in the using process can be reduced.

Preferably, the compound protease is composed of the following components in parts by weight: 10-20 parts of papain and 30-50 parts of trypsin. The papain is beneficial to enzymolysis of components of the shrimp shell and the shrimp meat in the antarctic krill, the addition amount of the papain must be strictly controlled, and if the addition amount is too small, one-time enzymolysis is incomplete, and the subsequent enzymolysis effect is influenced; the addition amount is excessive, and unnecessary production cost is increased.

Preferably, in the step (2), the addition amount of the compound protease is 0.3-0.8% of the weight of the Antarctic krill.

Preferably, in the step (3), the addition amount of the flavourzyme is 2-3% of the weight of the antarctic krill. The flavourzyme can degrade the polypeptide into amino acid, the yield of the amino acid in the enzymolysis product is improved, meanwhile, the bitter peptide substances in the enzymolysis product in the first step are removed, the bitter taste of the enzymolysis product is removed, meanwhile, the addition amount of the flavourzyme must be strictly controlled, and the addition amount is too small, so that the secondary enzymolysis is incomplete, the yield of the amino acid is reduced, and the enzymolysis product has bitter taste; excessive addition results in waste of enzyme and unnecessary production costs.

Preferably, in the step (2), the pH value is controlled to be 8-9 and the temperature is controlled to be 45-50 ℃ in the enzymolysis process.

Preferably, in the step (3), the pH value is controlled to be 6-7 and the temperature is controlled to be 25-35 ℃ in the enzymolysis process.

Preferably, in the step (4), the water bath temperature is controlled to be 37-40 ℃, and the rotation speed of the high-speed centrifugation is controlled to be 18000-20000 r/min.

Therefore, the invention has the following beneficial effects: by adopting a step-by-step hydrolysis method, the papain is beneficial to the enzymolysis of the components of the shrimp shell and the shrimp meat in the antarctic krill; the flavourzyme can remove bitter taste, improve mouthfeel and increase the content of amino acid in the shrimp dissolving pulp.

Detailed Description

The technical solution of the present invention is further specifically described below by way of specific examples.

In the present invention, all the equipment and materials are commercially available or commonly used in the art, and the methods in the following examples are conventional in the art unless otherwise specified.