阅读说明：本技术 一种刺五加茎皮黄酮提取工艺及其应用 (Extraction process and application of acanthopanax bark flavone ) 是由 苏建青 阚琦缤 褚秀玲 于 2019-11-11 设计创作，主要内容包括：本发明属于生物化学技术领域,尤其涉及一种刺五加茎皮黄酮提取工艺及其应用。本发明首次提出对刺五加茎皮部位的黄酮进行超声波辅助乙醇提取工艺,并提供了一套能够较大程度从刺五加茎皮中提取黄酮的工艺,具体为：乙醇浓度50％,提取时间65min,液料比40mL/g,提取温度65℃,吐温80浓度1.8％(体积分数),提取功率300W的超声波提取工艺,优化后的黄酮提取率为24.75mg/g。(The invention belongs to the technical field of biochemistry, and particularly relates to an extraction process of acanthopanax bark flavone and application thereof. The invention provides an ultrasonic-assisted ethanol extraction process for the flavone in the bark of the acanthopanax senticosus for the first time, and provides a set of processes capable of extracting the flavone from the bark of the acanthopanax senticosus to a greater extent, which specifically comprises the following steps: the ethanol concentration is 50%, the extraction time is 65min, the liquid-material ratio is 40mL/g, the extraction temperature is 65 ℃, the Tween 80 concentration is 1.8% (volume fraction), the extraction power is 300W, and the optimized flavone extraction rate is 24.75 mg/g.)

1. The extraction process of the acanthopanax bark flavone is characterized by comprising the following steps:

step 1: cleaning dried acanthopanax bark, drying, crushing, and sieving with a 40-mesh sieve to obtain acanthopanax bark powder;

step 2: putting the acanthopanax bark powder into an ethanol solution, wherein the ethanol solution contains Tween 80, and carrying out ultrasonic extraction;

and step 3: filtering with 100 mesh filter screen to obtain filtrate containing flavone.

2. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: in step 1, the acanthopanax bark is dried for 24h at 60 ℃.

3. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: and (3) screening the acanthopanax bark powder obtained in the step (1), and adding dry powder into acanthopanax bark which can pass through a 60-mesh sieve but cannot pass through a 40-mesh sieve for extraction.

4. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: the volume percentage concentration of the ethanol used in the step 2 is 45-75%, and the volume percentage content of the Tween 80 in the ethanol solution is 0.23-0.36%.

5. The extraction process of acanthopanax bark flavone as claimed in claim 4, wherein the extraction process comprises the following steps: in the step 2, the liquid-material ratio of the acanthopanax bark powder to the ethanol solution is (15-40) to 1.

6. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: the power of the ultrasonic wave used in the step 2 is 150-400W.

7. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: the extraction temperature in step 2 is 45-70 ℃.

8. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: the extraction time in step 2 is 25-75 min.

9. The extraction process of acanthopanax bark flavone as claimed in claim 1, wherein the extraction process comprises the following steps: the ethanol used in the step 2 has the concentration of 50 percent, the extraction time is 65min, and the liquid-material ratio is 40:1, the extraction temperature is 65 ℃, the ultrasonic power is 300W, and the Tween 80 is 1.8 percent.

10. The use of the process for extracting flavones from Acanthopanax senticosus stem bark as claimed in any one of claims 1 to 9 in the extraction of flavones from Acanthopanax senticosus stem bark.

Technical Field

The invention belongs to the technical field of biochemistry, and particularly relates to an extraction process of acanthopanax bark flavone and application thereof.

Background

Acanthopanax senticosus (Rupr. et Maxim.) Harms belongs to Araliaceae plants, is 1-6 m high and has many branches, grows in forests or shrub clusters with the elevation of hundreds of meters to 2000 meters, and is mainly distributed in the northeast three provinces and the Korean provinces. Acanthopanax root is warm in nature, pungent and slightly bitter in taste; it has long history of application as a medicine for spleen, kidney and heart meridians. The Ming Dynasty Li Shizhen is the "Ben Cao gang mu" in which the acanthopanax root is the "superior" in the main channel, has the functions of tonifying middle-jiao and Qi, strengthening bones and muscles, strengthening will, reducing weight and resisting aging after long-term taking, and is mainly used for the symptoms of spleen-kidney yang deficiency, body weakness and hypodynamia, inappetence, soreness of waist and knees, insomnia and dreaminess, etc. Modern researches find that the main chemical components of acanthopanax comprise flavonoids, glycosides, polysaccharides, polyphenol compounds, sodium, potassium, selenium, iron and other trace elements, wherein the flavonoids are one of the main active components, can dilate coronary arteries, increase coronary blood flow, has the effects of slightly slowing heart rate, inhibiting cardiac contractility, reducing oxidative stress, inhibiting inflammatory factors and the like, and is used for the treatment and health care of livestock and poultry with anti-inflammation, anti-oxidation and anti-stress in the production of livestock and poultry.

In recent years, with the gradual withdrawal of antibiotics from the history of livestock and poultry breeding, the traditional Chinese medicines are rapidly developed in the aspect of preventing and treating livestock and poultry diseases, and the demand for acanthopanax senticosus is gradually increased, so that the phenomenon of drug conflict between livestock and poultry and people is caused, and therefore, the development of a new acanthopanax senticosus medicinal source or medicinal part is a way for solving the problem of insufficient supply of acanthopanax senticosus. At present, the part used as medicine specified in pharmacopoeia is dried root and rhizome of acanthopanax, and leaves and fruits of acanthopanax are also used in acanthopanax tea, acanthopanax health drink and the like. The stem or the bark of acanthopanax senticosus is not well applied and can generate a large amount of stems or bark of acanthopanax senticosus every year as a shrub, so that the research on the medicinal value and the effective component content of the stem and the bark of acanthopanax senticosus is of great significance.

Earlier studies found that the content of flavone in the bark of acanthopanax senticosus was significantly higher than that in the stem of acanthopanax senticosus, the content of bark of acanthopanax senticosus was 12.37%, while the content of stem was only 8.25% (water extraction result under the same conditions). The acanthopanax bark is the part with more potential development as medicinal value. The effective component flavone in the acanthopanax bark is more fully extracted, which is the key for efficiently utilizing the acanthopanax bark, and the method has important significance for protecting wild acanthopanax bark resources.

At present, the extraction parts of the effective components of acanthopanax are mainly concentrated on the root and rhizome of medicinal parts, and the acanthopanax fruits or acanthopanax leaves of health care products are concentrated on the root and rhizome of acanthopanax, but the reports of acanthopanax bark are few. The extraction method is also the traditional hot water extraction or ethanol reflux, which not only wastes time and labor, but also causes the decomposition of effective components due to high temperature.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides an extraction process of acanthopanax bark flavone and application thereof.

The invention is realized in this way, a process for extracting flavones from acanthopanax bark comprises the following steps:

step 1: cleaning dried acanthopanax bark, drying and crushing to obtain acanthopanax bark powder;

step 2: putting the acanthopanax bark powder into an ethanol solution, and carrying out ultrasonic extraction;

and step 3: filtering with 100 mesh filter screen to obtain filtrate containing flavone.

Further, in step 1, the bark of Acanthopanax senticosus is dried at 60 ℃ for 24 hours.

Further, the acanthopanax bark powder obtained in the step 1 is screened, and the acanthopanax bark dry powder which can pass through a 60-mesh sieve but can not pass through a 40-mesh sieve is reserved for extraction operation.

Further, the concentration of the ethanol used in the step 2 is 45-75%.

Furthermore, in the step 2, the liquid-to-material ratio of the acanthopanax bark powder to the ethanol solution is (15-40): 1.

Further, the power of the ultrasonic wave used in step 2 is 150-.

Further, the extraction temperature in step 2 is 45-70 ℃.

Further, the extraction time in step 2 is 25-75 min.

Further, tween 80 was 1.8% by volume in step 2.

Further, the ethanol concentration used in the step 2 is 50%, the extraction time is 65min, and the liquid-material ratio is 40:1, the extraction temperature is 65 ℃, the ultrasonic power is 300W, and the volume percentage of Tween 80 is 1.8%.

The application of the acanthopanax bark flavone extracting process in acanthopanax flavone extraction is described above.

In summary, the advantages and positive effects of the invention are:

the invention provides a process for extracting flavone from acanthopanax bark, wherein the acanthopanax bark is used as a non-utilized part of the acanthopanax Chinese medicine and is wasted as waste every year, and the health care or medicinal value of the acanthopanax bark is researched and developed, so that the overall utilization rate of the acanthopanax bark medicinal plant is improved, and the clinical treatment and health care medicine of livestock and poultry can be solved; the extraction method of acanthopanax specified in pharmacopoeia is hot water extraction or ethanol reflux extraction, which not only has long extraction time, but also wastes time and labor, and can cause the inactivation of active ingredients due to high temperature. At present, new extraction methods such as ultrasonic extraction, microwave extraction and ultrahigh pressure extraction are utilized, and the new extraction methods have the advantages of trouble saving, labor saving, low extraction temperature, high extraction efficiency and the like. The invention adopts a novel extraction method of ultrasonic-assisted ethanol, and adds surfactant Tween 80 which can improve the extraction rate of flavone to promote the release of the flavone, so as to obtain the optimal extraction process of the flavone from the acanthopanax bark, and provide support for the clinical development of the acanthopanax bark. The specific procedures are as follows: the ethanol concentration is 50%, the extraction time is 65min, the liquid-material ratio is 40mL/g, the extraction temperature is 65 ℃, the extraction power is 300W, the Tween 80 volume percent is 1.8%, and the optimized flavone extraction rate is 24.75 mg/g.

The research adopts novel ultrasonic wave to assist ethanol extraction, can shorten the extraction time, avoids the damage of high temperature to effective components, and has higher flavone extraction rate compared with the traditional extraction method.

Drawings

FIG. 1 is a graph of the effect of ethanol concentration on the extraction of acanthopanax senticosus flavonoids;

FIG. 2 is the effect of extraction time on the extraction of acanthopanax senticosus flavones;

FIG. 3 shows the effect of liquid-to-liquid ratio on the extraction rate of eleutheroside;

FIG. 4 is the effect of extraction temperature on the extraction rate of flavonoids from Acanthopanax senticosus;

FIG. 5 is the effect of ultrasonic power on the extraction of acanthopanax senticosus flavonoids;

FIG. 6 is the effect of Tween 80 concentration on extraction yield;

FIG. 7 is a graph showing the effect of ethanol concentration and extraction time on the extraction of flavonoids from Acanthopanax senticosus;

FIG. 8 shows the effect of ethanol concentration and liquid-to-liquid ratio on the extraction of eleutheroside;

FIG. 9 shows the effect of extraction time and liquid-to-liquid ratio on the extraction of eleutherococcus senticosus flavones.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the equipment and reagents used in the examples and test examples are commercially available without specific reference. The specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.

The invention discloses an extraction process of acanthopanax bark flavone and application thereof, which are shown in the following embodiments.