阅读说明：本技术 盐肤木总酚酸微丸在制备预防和/或治疗心血管疾病药物中的用途 (Application of rhus chinensis total phenolic acid pellets in preparation of medicines for preventing and/or treating cardiovascular diseases ) 是由 徐伟 叶淼 王晓颖 许文 禇克丹 林羽 于 2019-10-31 设计创作，主要内容包括：本发明提供了盐肤木总酚酸微丸在制备预防和/或治疗心血管疾病的药物中的用途。实验证明,发明制得的盐肤木总酚酸微丸可抑制心电图ST段的抬高和改善T波的变化,能显著降低心肌缺血阳性率和心律失常发生率,说明盐肤木总酚酸微丸对心血管疾病,特别是心律失常具有明显的预防保护作用。所以,盐肤木总酚酸微丸在制备预防和/或治疗心血管疾病,特别是心律失常的药物中具有非常好的应用前景。(The invention provides application of rhus chinensis total phenolic acid pellets in preparation of a medicine for preventing and/or treating cardiovascular diseases. Experiments prove that the prepared rhus chinensis total phenolic acid pellets can inhibit the elevation of an ST segment of an electrocardiogram and improve the change of T waves, can obviously reduce the myocardial ischemia positive rate and the arrhythmia incidence rate, and show that the rhus chinensis total phenolic acid pellets have obvious prevention and protection effects on cardiovascular diseases, particularly arrhythmia. Therefore, the rhus chinensis total phenolic acid pellets have good application prospect in preparing medicaments for preventing and/or treating cardiovascular diseases, particularly arrhythmia.)

1. Application of total phenolic acid pellet of Rhus chinensis Mill in preparing medicine for preventing and/or treating cardiovascular diseases is provided.

2. Use according to claim 1, characterized in that: the cardiovascular diseases include coronary heart disease, myocardial ischemia, and heart disease.

3. Use according to claim 2, characterized in that: the myocardial ischemia is acute myocardial ischemia.

4. Use according to claim 1, characterized in that: the cardiovascular disease is arrhythmia.

5. Use according to claim 1, characterized in that: the medicine can reduce the change of ST segment of electrocardiogram and reduce the incidence rate of arrhythmia.

6. The use of any one of of claims 1-5, wherein the total phenolic acid of Rhus chinensis Mill is prepared by collecting total phenolic acid of Rhus chinensis Mill, and making into pellet.

7. Use according to claim 6, characterized in that: the preparation method of the rhus chinensis total phenolic acid pellet comprises the following steps:

(1) adding organic solvent into Rhus chinensis Mill, extracting, and filtering to obtain filtrate;

(2) eluting and purifying the filtrate obtained in the step (1) by using macroporous resin as an adsorption filler: collecting the eluted part of the alcohol solvent by using water as an eluent and using the alcohol solvent as an eluent, concentrating, freezing and drying to obtain the rhus chinensis total phenolic acid;

(3) mixing the rhus chinensis total phenolic acid obtained in the step (2), a balling promoter, a pore-forming agent, an anti-sticking agent and a wetting agent, and preparing the rhus chinensis total phenolic acid pellets in a low-temperature extrusion spheronizer.

8. Use according to claim 7, characterized in that: in the step (1), the rhus chinensis is rhus chinensis tubers; the organic solvent is ethanol, preferably 70% ethanol; the mass volume ratio of the rhus chinensis to the organic solvent is 1: (10-30) g/mL;

and/or the extraction temperature is 70-90 ℃, and the extraction time is 60-120 minutes; preferably, the mass volume ratio of the rhus chinensis to the ethanol is 1: 20 g/mL; the extraction temperature is 80 ℃, and the extraction time is 90 minutes;

and/or in the step (2), the using amount of the water is 4BV, the using amount of the alcohol solvent is 4BV, and the alcohol solvent is 50% ethanol;

and/or the macroporous resin is SP825L macroporous resin, the sample loading amount is 0.35g rhus chinensis/g macroporous resin, and the flow rate of the eluent is 2 BV.h^-1。

9. Use according to claim 8, characterized in that: in the step (3), the balling accelerant is a cellulose balling accelerant, the pore-forming agent is a water-soluble pore-forming agent, the anti-sticking agent is an inorganic non-metal oxide, and the wetting agent is an alcohol substance, preferably, the balling accelerant is microcrystalline cellulose, the pore-forming agent is lactose, the anti-sticking agent is silicon dioxide, and the wetting agent is 60% ethanol;

the process conditions of the low-temperature extrusion rounding machine are as follows: extrusion speed 43r min^-1Speed of rounding is 1800 r.min^-1And the rounding time is 4 min.

10. Use according to claim 9, characterized in that: in the step (3), the mass volume ratio of the rhus chinensis total phenolic acid to the balling promoter to the pore-forming agent to the anti-sticking agent to the wetting agent is 45 g: 94.5 g: 10.5 g: 6.75 g: 110 mL.

Technical Field

The invention belongs to the field of medicine preparation, and particularly relates to application of rhus chinensis total phenolic acid pellets in preparation of medicines for preventing and/or treating cardiovascular diseases, particularly arrhythmia.

Background

Coronary Heart Disease (CHD) is kinds of common cardiovascular diseases, which are short for Coronary atherosclerotic heart disease, important marks of the CHD are atherosclerotic plaques, which can cause chronic stenosis, spasm or obstruction of Coronary arteries with the passage of time, thereby causing myocardial ischemia, hypoxia or infarction to cause heart disease, acute myocardial ischemia is kinds of myocardial ischemia, and means relative or absolute insufficient blood supply of myocardium and myocardial cell hypoxia caused by various reasons.

Arrhythmia is the most common complex and dangerous cardiovascular disease or complication, is causing high mortality of cardiovascular disease, can be singly attacked, and can also be accompanied with other cardiovascular diseases, the clinical manifestations of arrhythmia are palpitation, shortness of breath, chest distress, hypodynamia, insomnia, dreaminess and other symptoms, and the existing medicines for treating arrhythmia include amiodarone, lidocaine, Diaoxinxuekang capsules and the like.

However, most of the existing medicines for treating cardiovascular diseases such as coronary heart disease and arrhythmia have side effects, and the traditional Chinese medicines are concerned with due to relatively small side effects, in recent years, the research on the cardiovascular diseases by the traditional Chinese medicines is increasing, people have better effects of preventing and treating the cardiovascular diseases by applying the traditional Chinese medicines, and corresponding traditional Chinese medicine preparations are continuously developed.

However, the basis of the drug effect substance of rhus chinensis for radically treating coronary heart disease is not clear up till now. So far, scholars at home and abroad also have insufficient research on chemical components of rhus chinensis roots, so that the research on the chemical components in the rhus chinensis roots is of great significance in finding medicines capable of effectively treating cardiovascular diseases (such as coronary heart disease, acute myocardial ischemia, arrhythmia, heart disease caused by anoxia or infarction, and the like).

Disclosure of Invention

The invention aims to provide application of rhus chinensis total phenolic acid pellets in preparation of medicines for preventing and/or treating cardiovascular diseases, in particular arrhythmia.

The invention provides application of types of rhus chinensis total phenolic acid pellets in preparation of medicines for preventing and/or treating cardiovascular diseases.

, the cardiovascular diseases include coronary heart disease, myocardial ischemia, and heart disease.

Further , the myocardial ischemia is acute myocardial ischemia.

Further , the cardiovascular disease is arrhythmia.

, the medicine can reduce ST segment change of electrocardiogram and reduce arrhythmia incidence.

the preparation method comprises collecting total phenolic acid of Rhus chinensis Mill, and making into pellet.

, the preparation method of the rhus chinensis total phenolic acid pellet comprises the following steps:

(1) adding organic solvent into Rhus chinensis Mill, extracting, and filtering to obtain filtrate;

(2) eluting and purifying the filtrate obtained in the step (1) by using macroporous resin as an adsorption filler: collecting the eluted part of the alcohol solvent by using water as an eluent and using the alcohol solvent as an eluent, concentrating, freezing and drying to obtain the rhus chinensis total phenolic acid;

(3) mixing the rhus chinensis total phenolic acid obtained in the step (2), a balling promoter, a pore-forming agent, an anti-sticking agent and a wetting agent, and preparing the rhus chinensis total phenolic acid pellets in a low-temperature extrusion spheronizer.

, in the step (1), the rhus chinensis is rhus chinensis tubers, the organic solvent is ethanol, preferably 70% ethanol, and the mass-volume ratio of the rhus chinensis to the organic solvent is 1 (10-30) g/mL;

and/or the extraction temperature is 70-90 ℃, and the extraction time is 60-120 minutes; preferably, the mass volume ratio of the rhus chinensis to the ethanol is 1: 20 g/mL; the extraction temperature is 80 ℃, and the extraction time is 90 minutes.

, in the step (2), the using amount of the water is 4BV, the using amount of the alcohol solvent is 4BV, and the alcohol solvent is 50% ethanol;

and/or the macroporous resin is SP825L macroporous resin, the sample loading amount is 0.35g rhus chinensis/g macroporous resin, and the flow rate of the eluent is 2 BV.h^-1。

, in step (3), the balling accelerant is a cellulose balling accelerant, the pore-forming agent is a water-soluble pore-forming agent, the anti-sticking agent is an inorganic non-metal oxide, and the wetting agent is an alcohol substance, preferably, the balling accelerant is microcrystalline cellulose, the pore-forming agent is lactose, the anti-sticking agent is silicon dioxide, and the wetting agent is 60% ethanol;

the process conditions of the low-temperature extrusion rounding machine are as follows: extrusion speed 43r min^-1Speed of rounding is 1800 r.min^-1And the rounding time is 4 min.

, in the step (3), the mass volume ratio of the rhus chinensis total phenolic acid to the balling promoter to the pore-forming agent to the anti-sticking agent to the wetting agent is 45g to 94.5g to 10.5g to 6.75g to 110 mL.

In the present invention, the pellets are spherical or spheroidal solid dosage forms having a diameter of less than 2.5mm, and can be filled into capsules, compressed into tablets, or made into other formulations.

The experimental result shows that the rhus chinensis total phenolic acid pellet prepared by the invention can inhibit the elevation of an ST segment of an electrocardiogram and improve the change of T wave, can obviously reduce the myocardial ischemia positive rate and the arrhythmia incidence rate, and particularly has the effect of reducing the arrhythmia incidence rate of myocardial ischemia rats which is obviously superior to that of a YFM total phenolic acid group. The rhus chinensis total phenolic acid pellets have obvious prevention and protection effects on cardiovascular diseases, particularly arrhythmia. Therefore, the rhus chinensis total phenolic acid pellets have good application prospect in preparing medicaments for preventing and/or treating cardiovascular diseases, particularly arrhythmia.

Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.

The present invention will be described in further detail by way of examples , but it should not be construed that the scope of the above subject matter is limited to the examples described below.

Drawings

Fig. 1 shows typical electrocardiograms of each group.

Detailed Description

The raw materials and equipment used in the invention are known products and are obtained by purchasing commercial products.