阅读说明：本技术 一种扶正解毒复合制剂及其制备方法和应用 (Compound preparation for strengthening body resistance and detoxifying, and preparation method and application thereof ) 是由 张世新 万宗敏 陈波 贾付从 于 2018-06-15 设计创作，主要内容包括：本发明公开了一种扶正解毒复合制剂及其制备方法和应用,制备方法包括：S1：将乳酸菌、芽孢杆菌和将黑曲霉分别进行扩大培养,将扩大后的菌液按体积比乳酸菌菌液：芽孢杆菌菌液：黑曲霉菌液为1-4：2-4：1-3混合制得复合菌液；S2：按重量份数,取黄芪2-6份,板蓝根2-6份,淫羊藿2-4份,混合后于110-130℃下灭菌；S3：将复合菌液与灭菌后的中草药混合物按液固重量份比15-30：1混合后密闭发酵72-96h；S4：向发酵混合物中继续混入混合物总质量0.1-1.0%的促纤维素分解酶和混合物总质量0.1-1.0%的溶葡萄球菌酶,4-8h后,进行CO<Sub>2</Sub>超临界萃取,萃取物经冷冻干燥制得的成品改善了有效成分,提高禽畜机体免疫力。(The invention discloses a compound preparation for strengthening body resistance and detoxifying, a preparation method and application thereof, wherein the preparation method comprises the following steps: s1: respectively carrying out amplification culture on lactobacillus and bacillus and aspergillus niger, and carrying out volume ratio on the amplified bacterium liquid of the lactobacillus bacterium liquid: and (3) bacillus liquid: the aspergillus niger bacterial liquid is 1-4: 2-4: 1-3, mixing to obtain a compound bacterial liquid; s2: mixing 2-6 parts of astragalus, 2-6 parts of isatis root and 2-4 parts of epimedium according to parts by weight, and sterilizing at the temperature of 110-; s3: mixing the compound bacterial liquid and the sterilized Chinese herbal medicine mixture according to the weight ratio of liquid to solid of 15-30: 1, mixing and then fermenting in a closed manner for 72-96 h; s4: continuously mixing 0.1-1.0% of cellulolytic enzyme and 0.1-1.0% of lysostaphin into the fermented mixture, and performing CO treatment for 4-8 hr 2 The effective components of the finished product prepared by supercritical extraction and freeze drying of the extract are improved, and the organism immunity of the livestock is improved.)

1. A preparation method of a compound preparation for strengthening body resistance and detoxifying is characterized in that: the method comprises the following steps:

s1: respectively carrying out amplification culture on lactobacillus and bacillus and aspergillus niger, and then, carrying out amplification culture on the expanded bacterium liquid according to the volume ratio of the lactobacillus bacterium liquid: and (3) bacillus liquid: the ratio of the aspergillus niger liquid is 1-4: 2-4: 1-3, and preparing a compound bacterial liquid;

s2: mixing 2-6 parts of astragalus, 2-6 parts of isatis root and 2-4 parts of epimedium according to parts by weight, and sterilizing at the temperature of 110-;

s3: mixing the compound bacterial liquid and the sterilized Chinese herbal medicine mixture according to the weight ratio of liquid to solid of 15-30: 1, uniformly mixing, and fermenting in a sealed manner for 72-96 h;

s4: further mixing the amount of the mixture into the fermentation mixtureCellulolytic enzyme accounting for 0.1-1.0% of the total mass of the mixture and lysostaphin accounting for 0.1-1.0% of the total mass of the mixture, and performing CO treatment after 4-8h₂Performing supercritical extraction, and freeze drying the extract to obtain the finished product.

2. The method for preparing a compound preparation for strengthening body resistance and removing toxic substance according to claim 1, wherein the expanded culture of aspergillus niger in step S1 is: aspergillus niger is subjected to amplification culture with culture solution composed of 8-12% by mass of MAIZHU extract and 5-10% by mass of defatted soybean powder, pH6.5-6.8, and continuously fermented for 70-75 h.

3. The method for preparing a compound preparation for strengthening body resistance and removing toxicity according to claim 1, wherein the method comprises the following steps: the Chinese herbal medicines in the step S2 further comprise 2-4 parts of eucommia ulmoides and 2-4 parts of codonopsis pilosula according to parts by weight.

4. The method for preparing a compound preparation for strengthening body resistance and removing toxicity according to claim 1, wherein the method comprises the following steps: in step S3, the optimum temperature for sealed fermentation is 32-35 deg.C, and the enzyme production temperature is 28-30 deg.C.

5. The method for preparing a compound preparation for strengthening body resistance and removing toxicity according to claim 1, wherein the method comprises the following steps: in the step S3, a synergist is added during closed fermentation, wherein the synergist is composed of a mineral element Cu²⁺、Co²⁺、Mg²⁺、Fe²⁺、Zn²⁺、Ca²⁺The water-soluble compound of (a) is an aqueous solution prepared by the following element substances in ratio: cu²⁺：Co²⁺：Mg²⁺：Fe²⁺：Zn²⁺：Ca²⁺=1.0-6.25：1.5-5.0：20-100：18-89：38.5-115：250-500。

6. The method for preparing a compound preparation for strengthening body resistance and removing toxicity according to claim 5, wherein the method comprises the following steps: the water-soluble compound is CaCl₂，ZnSO₄，FeSO₄，CuSO₄，Co(NO₃)₂，MgSO₄。

7. The method for preparing a compound preparation for strengthening body resistance and removing toxicity according to claim 1, wherein the method comprises the following steps: the prepared finished product is one of granules, effervescent granules, dispersible tablets, effervescent tablets, film-coated tablets, pills, capsules, soft capsules, dripping pills, oral liquid and injection.

8. A complex formulation for strengthening body resistance and removing toxic substance prepared by the preparation method of any one of claims 1 to 7.

9. The use of the complex formulation for strengthening body resistance and removing toxic substance of claim 8, wherein: is applied to the field of livestock breeding.

10. The use of the complex formulation for strengthening body resistance and removing toxic substance as set forth in claim 9, wherein: can be used for improving pig immunity.

Technical Field

The invention relates to the technical field of large-scale breeding of livestock, in particular to a compound preparation for strengthening body resistance and detoxifying, and a preparation method and application thereof.

Background

In the process of livestock and poultry and aquaculture, the immune function is easily inhibited due to long-term extensive stress factors. Taking a pig as an example, in this case, various pathogenic factors such as viruses, bacteria and protozoa can invade and destroy the physiology and immune system functions of the pig, free radicals generated by organ and tissue metabolism and acidic substances such as lactic acid, keto acid and uric acid can cause long-term acid bias of pig body fluid (pH is between 7.31 and 7.36), and in this case, the blood viscosity of the pig is high, the fluidity is low, and the effect of cells can be weakened; the oxygen carrying and nutrient carrying capacity of red blood cells is reduced, the enzymatic reaction efficiency is reduced, the metabolism is slowed down, the physiological function is declined, the immune system function is low, and particularly, the nutrition cannot be well absorbed. Various chronic diseases which can not be cured for a long time can be caused.

Moreover, with the development of the breeding industry in China, extensive family feeding is gradually changed into specialized, intensive and high-density feeding, and the pig raising level is obviously improved. During the growth process of the pigs, the chronic poisoning, the increase of drug resistance and the reduction of resistance are caused by continuously and excessively adding antibiotics for a long time. In each breeding link, such as frequent herd combining and herd transferring, frequent material changing and people changing, and the like, the breeding environment has dirty air, drinking water pollution, overproof pathogenic microorganisms, and the like, and the pathogens and toxins firstly damage the digestive tract of animals in the animal body to cause digestive tract inflammation or ulcer, which is manifested as digestive disorders such as vomit, constipation, diarrhea and the like, and secondly damage the integrity of the respiratory tract mucous membrane to destroy the immune barrier of the respiratory tract mucous membrane, which is manifested as respiratory tract syndrome.

Furthermore, in the current breeding mode, the normal immune response of pigs is affected by long-term multiple-vaccine-disorganization, so that immune interference, immune tolerance and immune failure are caused. Heavy metal and mycotoxin in the feed exceed standards, and reproductive hormone is abused for a long time, so that the physical ability of the sow is reduced, the resistance is low, and the uterine contraction is weak; flushing and swelling vulva, vomiting, anorexia and diarrhea of the sow; the multiparous sow has no estrus, false estrus, abortion and stillbirth, insufficient milk, overlong birth process or repeated mating infertility. In obsolete sows which are frequently infested, more than 80 percent of the obsolete sows are caused by the reasons, so that the problems of the reduction of the immunity of animal organisms, the reduction of the quality of livestock and poultry products, the enhancement of the drug resistance of bacteria and the like are solved.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the compound preparation for strengthening body resistance and detoxifying, which improves the effective components of the compound preparation for strengthening body resistance and detoxifying and improves the organism immunity of livestock.

A preparation method of a compound preparation for strengthening body resistance and detoxifying comprises the following steps:

s1: respectively carrying out amplification culture on lactobacillus and bacillus and aspergillus niger, and then, carrying out amplification culture on the expanded bacterium liquid according to the volume ratio of the lactobacillus bacterium liquid: and (3) bacillus liquid: the ratio of the aspergillus niger liquid is 1-4: 2-4: 1-3, and preparing a compound bacterial liquid;

s2: mixing 2-6 parts of astragalus, 2-6 parts of isatis root and 2-4 parts of epimedium according to parts by weight, and sterilizing at the temperature of 110-;

s3: mixing the compound bacterial liquid and the sterilized Chinese herbal medicine mixture according to the weight ratio of liquid to solid of 15-30: 1, uniformly mixing, and fermenting in a sealed manner for 72-96 h;

s4: continuously mixing the fermentation mixture with 0.1-1.0% of cellulolytic enzyme and 0.1-1.0% of lysostaphin, and performing CO treatment for 4-8 hr₂Performing supercritical extraction, and freeze drying the extract to obtain the finished product.

By adopting the scheme, the Chinese herbal medicine is sterilized at high temperature before fermentation, so that the influence of mixed bacteria on the fermentation process is avoided. During fermentation, lactic acid, bacillus and aspergillus niger are selected as fermentation strains, almost complete sets of decomposition enzymes aiming at the botanical raw materials are contained, the decomposition enzymes are microorganisms with the strongest decomposition capability in the natural world, the toxicity of the Chinese herbal medicines is reduced through the powerful biotransformation function of the microorganisms, and natural plant cells are subjected to enzymolysis, so that the effective components in the Chinese herbal medicines are fully released freely and enriched. After fermentation, the added cellulolytic enzyme and lysostaphin can selectively degrade natural macromolecules, plant polypeptides and the like in the Chinese herbal medicines into micromolecular substances which are easily absorbed and utilized by organisms, such as various glycosides, alkaloids, polysaccharides, organic sugars, volatile oil and other immune active substances. CO 2₂The supercritical extraction process has low extraction temperature, and can reduce the damage to effective components of Chinese medicinal materials as much as possible. The obtained product contains multiple immunocompetent substances, and can regulate nerve, body fluid and cell molecules of organism in all directions, and activate cell and humoral immunity system of organismHas effects in improving disease resistance of livestock and fowl, clearing away heat and toxic materials, strengthening body resistance, and eliminating pathogenic factors.

Preferably, the expanded culture of aspergillus niger in step S1 is: aspergillus niger is subjected to amplification culture with culture solution composed of 8-12% by mass of MAIZHU extract and 5-10% by mass of defatted soybean powder, pH6.5-6.8, and continuously fermented for 70-75 h.

By adopting the scheme, the special culture solution is prepared for the aspergillus niger, so that the culture effect of the aspergillus niger is improved.

Preferably, the Chinese herbal medicines in the step S2 further include, by weight, 2-4 parts of eucommia ulmoides and 2-4 parts of codonopsis pilosula.

By adopting the scheme, the eucommia ulmoides is sweet in taste and warm in nature, has the effects of tonifying liver and kidney, strengthening tendons and bones, regulating Chong and ren meridians, strengthening channels and preventing miscarriage, and has the effects of tonifying middle-jiao and Qi, invigorating spleen and benefiting lung. Radix Codonopsis has effects of enhancing immunity, dilating blood vessel, lowering blood pressure, improving microcirculation, and improving hemopoiesis function. The addition of eucommia bark and radix codonopsitis can further improve the types of effective components in the compound preparation, and further can further improve the drug effect.

Preferably, the optimal temperature for the sealed fermentation in step S3 is 32-35 deg.C, and the enzyme production temperature is 28-30 deg.C.

Preferably, a synergist is added during the closed fermentation in step S3, wherein the synergist is a mineral element Cu²⁺、Co²⁺、Mg²⁺、Fe²⁺、Zn²⁺、Ca²⁺The water-soluble compound of (a) is an aqueous solution prepared by the following element substances in ratio: cu²⁺：Co²⁺：Mg²⁺：Fe²⁺：Zn²⁺：Ca²⁺＝1.0-6.25：1.5-5.0：20-100：18-89：38.5-115：250-500。

Preferably, the synergist is made of CaCl₂，ZnSO₄，Fe SO₄，CuSO₄，Co(NO₃)₂，MgSO₄Preparing the aqueous solution.

By adopting the scheme, the mineral elements can provide various nutrient components for the growth of microorganisms, improve the quantity of viable bacteria in the fermentation liquor, improve the stability and activity of the performance of the composite preparation, shorten the fermentation period and reduce the fermentation cost.

Preferably, the prepared finished product dosage form is one of granules, effervescent granules, dispersible tablets, effervescent tablets, film-coated tablets, pills, capsules, soft capsules, dripping pills, oral liquid and injection.

Object two of the present invention: provides a compound preparation for strengthening body resistance and detoxifying, which is prepared by the preparation method.

The third purpose of the invention is that: provides the application of the compound preparation for strengthening body resistance and detoxifying, and is applied to the field of livestock breeding.

Preferably, the feed additive is used for improving the immunity of pigs.

In conclusion, the invention has the following beneficial effects:

1. in the fermentation process, lactic acid bacteria, bacillus and aspergillus niger are selected, the synergistic effect of Chinese herbal medicines and probiotics is utilized, namely the Chinese herbal medicine active ingredients promote the proliferation efficiency of the probiotics, the probiotics carry out biotransformation on the Chinese herbal medicines to generate probiotic mycoprotein antibodies, oligosaccharides and derivatives thereof, and the probiotic mycoprotein antibodies, the oligosaccharides and the derivatives thereof have the effects of resisting inflammation and infection and promoting the propagation of intestinal beneficial flora and also have the effect of activating the immune function of the organism. Preventing and treating various viral respiratory diseases, hyperpyrexia syndrome and the like of pigs. The drug effect of active ingredients in the Chinese herbal medicine is improved;

2. the added cellulolytic enzyme and lysostaphin can selectively decompose natural macromolecules, plant polypeptides and the like in the Chinese herbal medicine to generate a plurality of immunocompetent substances such as glycosides, alkaloids, polysaccharides, organic sugars, volatile oil and the like, and the immunocompetent substances are matched with decomposition fermentation products to further improve the types and the activities of effective components in the Chinese herbal medicine and improve the utilization rate of the Chinese herbal medicine;

3. selecting CO₂The supercritical extraction process has low extraction temperature, and can reduce the damage to the effective components of the Chinese herbal medicines as much as possible compared with the traditional Chinese herbal medicine decoction extraction mode;

4. the compound preparation of the invention is not added with preservative, and is a novel compound Chinese herbal medicine microecological preparation which is efficient, green, residue-free, antibiotic-free and antibacterial. Can strongly improve immunity and construct multiple immunity barriers of livestock. Can be used as the first choice medicine for viral and bacterial respiratory diseases, and has effects of rapidly activating organism immunity, starting disease-resistant system, improving organism immunity and disease resistance, and reducing death rate. Has wide action range and quick curative effect, and can be added for a long time.

Drawings

FIG. 1 shows histological sections (400-fold) of livers of various groups of experimental piglets in a safety test for pigs as a target animal;

FIG. 2 is a histological section of spleen of each experimental group of piglets in safety test for target animal pigs (400-fold);

fig. 3 shows kidney histological sections (400-fold) of each group of experimental piglets in the safety test for the target animal pig.

Detailed Description