阅读说明：本技术 一种可提高畜禽饲料能量利用效率的复合酶技术及其应用 (Complex enzyme technology capable of improving energy utilization efficiency of livestock and poultry feed and application thereof ) 是由 宋全芳 余璐璐 李阳 严峰 彭翔 张广民 蔡辉益 于 2019-11-08 设计创作，主要内容包括：一种可提高畜禽饲料能量利用效率的复合酶及其应用,目的在于提高畜禽日粮能量利用效率。所述复合酶包括液态发酵酶,固态发酵复合酶和载体,所述复合酶以β-甘露聚糖酶为主,还包括木聚糖酶、β-葡聚糖酶、果胶酶、纤维素酶。所述复合酶中β-甘露聚糖酶活性为4000-8000U/g,木聚糖酶活性为2000-4000U/g,β-葡聚糖酶活性为400-800U/g,果胶酶活性为200-1000U/g,纤维素酶300-600U/g。本发明所述复合酶在低能日粮中应用,可保持动物机体对能量的正常需要。本发明产品具有绿色,环保,无抗等优点,具有较高的推广应用价值。(A complex enzyme capable of improving the energy utilization efficiency of livestock and poultry feed and an application thereof, aiming at improving the energy utilization efficiency of livestock and poultry daily ration. The compound enzyme comprises liquid fermentation enzyme, solid fermentation compound enzyme and a carrier, and the compound enzyme mainly comprises beta-mannase, and also comprises xylanase, beta-glucanase, pectinase and cellulase. In the complex enzyme, the activity of beta-mannase is 4000-. The complex enzyme is applied to low-energy daily ration, and can keep the normal requirement of animal organism on energy. The product of the invention has the advantages of green, environmental protection, no resistance and the like, and has higher popularization and application values.)

1. The composite non-starch polysaccharide enzyme capable of improving the energy utilization efficiency of livestock and poultry feed is characterized by comprising the following components in percentage by weight:

25% -50% of liquid fermentation single enzyme;

20-35% of solid-state fermentation complex enzyme;

15% -55% of a carrier;

the liquid fermentation enzyme comprises beta-mannase, xylanase, beta-glucanase and cellulase, wherein the activity of the beta-mannase is 4000-8000U/g, the activity of the xylanase is 1500-3500U/g, the activity of the beta-glucanase is 200-700U/g, the activity of the cellulase is 200-500U/g, and the activity of the solid fermentation complex enzyme is as follows: 3000-9000U/g xylanase, 1200-1200U/g beta-glucanase activity, 1800-2500U/g pectinase activity and 600-600U/g cellulase activity, wherein the carrier is corn starch.

2. The compound non-starch polysaccharide enzyme of claim 1, wherein the single-enzyme beta-mannase, xylanase and beta-glucanase are prepared by fermenting yeast, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis through a liquid fermentation process; the solid-state fermentation complex enzyme is produced by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and is prepared by adopting a deep solid-state fermentation process.

3. The use of the compound non-starch polysaccharidase according to claims 1-2 in a broiler feeding process.

4. The use of claim 3, wherein the complex non-starch polysaccharidase is used in a low energy ration formulation for broiler chickens.

5. The application of the compound non-starch polysaccharide enzyme as claimed in claim 3, wherein the compound non-starch polysaccharide enzyme is added in an amount of 100-300 g/ton complete feed in the raising process of broiler chickens.

Technical Field

The invention belongs to the field of feed additives, and particularly relates to a composite non-starch polysaccharide enzyme which is applied to low-energy daily ration and can effectively improve the energy utilization efficiency of feed and application thereof.

Background

With the continuous and high-speed development of the animal husbandry in China in recent years, the condition that main feed raw materials depend on import for a long time seriously influences the stability and development of the feed industry and the breeding industry in China. China has a large amount of grain processing byproducts (cotton seed meal, rapeseed meal, DDGS and the like), and the development and utilization of the non-grain resources are important ways for guaranteeing the continuous and healthy development of the animal husbandry in China. However, these byproducts often contain high-level non-starch polysaccharides (NSP) which exist as main anti-nutritional factors in the feed, so that the wide use of the byproducts in the breeding industry is limited, and the digestion and absorption of nutrients by livestock and poultry are also hindered.

Non-starch polysaccharides (NSP) are a generic term for all polysaccharide carbohydrates in plant tissues, except starch, lignin and small amounts of oligosaccharides, and mainly include cellulose, β -glucan, arabinoxylan, mannan, pectin and the like. The beta-mannan is hemicellulose which is widely distributed in feed raw materials such as corn, bean pulp, sesame pulp, coconut pulp, palm pulp and the like and is harmful to the digestion and absorption of animal nutrient substances, wherein the water-soluble beta-mannan is the hemicellulose with anti-nutrition, the anti-nutrition effect of the beta-mannan is far higher than that of xylan, glucan and the like, and the beta-mannan is mainly expressed as follows: increasing the viscosity of livestock and poultry intestinal chyme, so that the movement of intestinal contents is difficult; the interaction between digestive enzyme and substrate is blocked, the absorption of nutrients is physically blocked, and the water absorption is increased; the diffusion speed of the chyme to the surface layer of the mucous membrane is reduced, so that the absorption and utilization of nutrient substances are greatly influenced; in addition, β -mannan is a pathogen-associated molecular structure that is recognized by pattern recognition receptors in the gut to induce unwanted immune responses.

Most of the complex enzymes in the current market are prepared by combining single enzymes fermented in liquid state. Liquid fermentation is a domestic mainstream fermentation mode, and has the advantages of high production efficiency, sufficient sterilization and the like, but the complex enzyme compounded by liquid fermentation is difficult to reach a natural enzyme combination system generated by microorganisms in a wild state. The enzymes produced by solid state fermentation are rich in species, are adapted to the complexity of plant tissues, are more suitable for feeds, and destroy natural plant cell walls with complex chemical structures under the action of a complex enzyme system. At present, the research on the combined use of the solid fermentation enzyme and the liquid fermentation enzyme is less in China, and the research shows that the addition of the liquid fermentation single enzyme in the solid fermentation enzyme has obvious effects on daily gain and feed intake of nursery pigs, and the growth performance and the weight gain cost are superior to those of the liquid fermentation combined compound enzyme.

Therefore, the inventor utilizes a monogastric animal bionic digestion system and a broiler chicken feeding test to carry out combined effect research on complex enzyme mainly comprising beta-mannase and solid fermentation complex enzyme in vitro and in vivo and optimizes the proportional composition of the complex enzyme. The invention can obviously improve the energy utilization efficiency of livestock and poultry, reduce the production cost of livestock and poultry and improve the production performance of livestock and poultry.

Disclosure of Invention

Based on the shortage of energy feed raw materials in the current market, the invention aims to provide the feed additive which is applied to the daily ration of livestock and poultry and is used for improving the feed energy utilization efficiency and the application thereof. The complex enzyme product can improve the livestock and poultry breeding environment and achieve the purpose of improving the production performance.

The compound enzyme is formed by combining liquid fermentation enzyme and solid fermentation enzyme, wherein the mass ratio of the liquid fermentation enzyme is 25-50%, the mass ratio of the solid fermentation enzyme is 20-35%, and the mass ratio of the corn starch carrier is 15-55%.

The complex enzyme is prepared by compounding liquid-state fermented beta-mannase, xylanase, beta-glucanase, cellulase and solid-state fermented complex enzyme in proportion, wherein the activity of the beta-mannase is 8000U/g for 4000-one-year-old xylanase, the activity of the xylanase is 2000-4000U/g for 400-one-year-old 800U/g, the activity of pectinase is 1000U/g for 200-one-year-old and the activity of the cellulase is 600-one-year-old 600U/g.

Preferably, the activity of beta-mannase in the complex enzyme is 5000U/g, the activity of xylanase is 3000U/g, the activity of beta-glucanase is 600U/g, the activity of pectinase is 600U/g, and the activity of cellulase is 400U/g.

In the complex enzyme, the activity of beta-mannase is 4000-.

The xylanase activity of the solid fermentation enzyme in the complex enzyme is 3000-9000U/g, the beta-glucanase activity is 500-1200U/g, the pectinase activity is 1800-2500U/g, the cellulase activity is 300-600U/g, preferably, the xylanase activity is 4000U/g, the beta-glucanase activity is 800U/g, the pectinase activity is 2400U/g, and the cellulase activity is 400U/g.

The beta-mannase, the xylanase and the beta-glucanase are prepared by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and adopting a liquid submerged fermentation process.

The solid-state fermentation complex enzyme is produced by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and is prepared by adopting a solid-state submerged fermentation process.

The compound enzyme is composed of a plurality of single enzymes and solid state fermentation compound enzyme according to the optimal proportion; meanwhile, compared with the pure solid state fermentation enzyme and the pure liquid state fermentation enzyme, the compound enzyme has more excellent effect; the compound enzyme combination is finally determined by a large number of bionic digestion tests by the inventor and has a crucial influence on the effect of the invention.

The complex enzyme can be applied to the feeding process of broiler chickens, and more preferably, is applied to a low-energy daily ration formula of broiler chickens.

In a preferred embodiment of the invention, the final addition amount of the complex enzyme is 100-300 g/ton of complete feed.

The complex enzyme disclosed by the invention is applied to low-energy daily ration of broiler chickens, the feed cost can be obviously reduced, the production performance of the broiler chickens can be improved, the in-vitro effect of the complex enzyme disclosed by the invention has a remarkable effect when being applied to the low-energy daily ration, and the complex enzyme has a strong economic application benefit and a market popularization value.

Detailed Description