阅读说明：本技术 一种火麻蜂蜜粉的制备方法 (Preparation method of fructus cannabis and honey powder ) 是由 郭春景 穆竟 赵金海 赵立涛 贾君 常存 刘峰 尤秋实 曹彦 于 2019-10-10 设计创作，主要内容包括：本发明属于火麻食品制备技术领域,具体涉及一种火麻蜂蜜粉的制备方法。本发明包括以下步骤：(1)调配料液；(2)均质,将料液进行均质；(3)乳化,料液中加入乳化剂,充分搅拌获得混合物,用剪切机对混合物告诉剪切进行乳化；(4)喷雾干燥。本发明增加蜂蜜了营养价值,产品中所含有的多糖具有抗癌、抗炎、保护心血管、提高身体免疫力等多种功效作用,尤其火麻多糖,还具有润肠通便、排毒等作用。本发明料液中添加的麦芽糖糊精、β-环糊精、明胶以及火麻胶、火麻多糖溶解性好,吸湿性低,起到降低蜂蜜粉吸湿性的作用。(The invention belongs to the technical field of preparation of fructus cannabis foods, and particularly relates to a preparation method of fructus cannabis honey powder. The invention comprises the following steps: (1) mixing the ingredient liquid; (2) homogenizing, namely homogenizing the feed liquid; (3) emulsifying, adding an emulsifier into the feed liquid, fully stirring to obtain a mixture, and performing high-speed shearing on the mixture by using a shearing machine to emulsify; (4) and (5) spray drying. The honey disclosed by the invention is added with the nutritional value, and the polysaccharide contained in the honey has various effects of resisting cancer, resisting inflammation, protecting cardiovascular system, improving body immunity and the like, and particularly the hemp polysaccharide also has the effects of relaxing bowel, expelling toxin and the like. The maltodextrin, the beta-cyclodextrin, the gelatin, the hemp gum and the hemp polysaccharide added in the feed liquid have good solubility and low hygroscopicity, and play a role in reducing the hygroscopicity of the honey powder.)

1. A preparation process of hemp honey powder is characterized by comprising the following steps:

(1) mixing the ingredient liquid, weighing 10-13 parts of maltodextrin, 1-3 parts of beta-cyclodextrin, 1-3 parts of gelatin and 5-8 parts of fructus cannabis polysaccharide according to parts by weight, adding water, mixing uniformly, adding 80-95 parts of honey, mixing uniformly, adding water to adjust the solid content to 20-30% to obtain a feed liquid; the fructus cannabis polysaccharide is prepared by the following steps:

(1.1) weighing 700g of hemp seeds, peeling, performing microwave treatment for detoxification, and crushing the hemp seeds into powder;

(1.2) soaking in 75% ethanol solution for degreasing, continuously stirring for 35min by using a magnetic stirrer, then centrifuging for 35min by using a centrifugal machine with the rotating speed of 4500r/min, removing supernatant, and collecting precipitate to obtain degreased powder;

(1.3) repeating the step (1.2) for more than four times;

(1.4) extracting the degreased powder by using 800ml of distilled water at room temperature, continuously stirring for 3 hours, centrifuging for 45 minutes by using a centrifugal machine with the rotating speed of 4500r/min, and collecting supernatant;

(1.5) repeating the step (1.4) for more than 3 times, combining all the supernatants, and concentrating under reduced pressure to obtain a fructus cannabis extract concentrated solution;

(1.6) adding a buffer solution and a bacteriostatic agent toluene, adjusting the pH value of the concentrated solution to be between 5.5 and 6, controlling the temperature to be between 36 and 37 ℃, heating to inactivate enzyme, performing 72-hour running water dialysis by using a dialysis bag to remove small molecular impurity pigment, and performing freeze drying on the liquid in the dialysis bag to obtain a dried crude polysaccharide;

(2) homogenizing, namely homogenizing the feed liquid;

(3) emulsifying, adding an emulsifier into the feed liquid, fully stirring to obtain a mixture, and performing high-speed shearing on the mixture by using a shearing machine to emulsify;

(4) spray drying, wherein the emulsified feed liquid is subjected to spray drying, the gas pressure is 0.1MPa, the air inlet temperature is 170-190 ℃, the air outlet temperature is 70-80 ℃, and the feeding amount is 5-8ml/s, so as to obtain the honey powder.

2. The preparation process of the hemp honey powder of claim 1, wherein 2-3 parts of hyssop extract is added into the blending liquid.

3. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: 2-5 parts of isomaltulose is added into the blending liquid.

4. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: 1-2 parts of cymbidium tincture are added into the blending liquid.

5. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: the preparation process of the hemp honey powder according to claim 1, which is characterized in that: the air inlet temperature is 190 ℃, the air outlet temperature is 80 ℃, and the feeding amount is 6 ml/s.

6. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: the air inlet temperature is 170 ℃, and the air outlet temperature is 8 ml/s.

7. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: the emulsifier is one or more of modified starch, soybean lecithin, sodium caseinate, sucrose ester or whey protein.

8. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: the shearing emulsification adopts a shearing emulsifying machine, the emulsifying time is 3-5min, and the emulsifying speed is 9000-11000 r/min.

9. The preparation process of the hemp honey powder according to claim 1, which is characterized in that: and carrying out two-stage homogenization by using a high-pressure homogenizer, wherein the first homogenization pressure is 40-50Mpa, and the second homogenization pressure is 20-30 Mpa.

Technical Field

The invention belongs to the technical field of preparation of fructus cannabis foods, and particularly relates to a preparation method of fructus cannabis honey powder.

Background

Honey is a viscous, transparent or translucent glue-packed liquid, the main components of which are monosaccharides such as glucose and fructose, which are easily absorbed by the human body, and in addition, honey contains various nutrients required by cells, tissues and organs of the human body, and can generate a large amount of heat without containing fat, so honey is considered as a natural tonic. The honey is used as a high-viscosity colloidal fluid, acidic components in the colloidal fluid can react with a metal container to cause corrosion, in addition, the honey is easily influenced by seasons and temperature, and the temperature is too high in summer, so that most of reducing sugar in the honey is fermented and converted into acid, and the storage time is limited; over-cooling in winter, if the temperature is below 15 ℃, honey is easy to generate incomplete crystallization, the mixing performance and the quality uniformity of the honey are affected, and great inconvenience is brought to the transportation, storage and eating of the honey. In order to solve the problems of high water content, strong moisture resistance, difficult storage and transportation, difficult guarantee of food sanitation, safety, stability and the like of liquid honey, the food processing industry develops and develops the honey powder which is prepared from the main raw materials with the taste of natural honey, not only retains the nutritional value of the liquid honey, but also is convenient for long-time transportation and storage and later deep processing and utilization. The common solidification technology of the honey powder is heating and baking, vacuum drying, vacuum freeze drying, crystallization method and heating and rolling method, the honey loses more functional components when heated for a long time, and the required period is long.

The hemp seeds are rich in nutrient components, contain 20-25% of protein, the protein mass of the soluble hemp seeds is about 30%, and the protein content of the shelled seeds is more than 40%. The hemp seed protein contains 21 amino acids, including 8 amino acids essential for human body. Nearly 2/3 of the hemp seed proteins are edestin which can promote the digestion and absorption of human body; further, 2/3 is albumin. The hemp seed protein has arginine of about 18.8mg/g and glycine of about 9.7mg/g, and has high content. Arginine can maintain the normal growth and development of infants. Most importantly, the hemp seed protein is easy to be absorbed by human body, and does not cause the advantages of gastrectasia, regurgitation, anaphylactic reaction and the like, so the hemp seed protein is very helpful for the nutrition supplement of infants, old people, sick and cured groups and weak and lean people. The hemp protein is hydrolyzed into protein peptide, and the protein peptide has physicochemical properties such as good solubility, computational or thermal stability and low viscosity which are not possessed by the hemp seed protein; and has various physiological functions of easy absorption, blood pressure reduction, blood sugar reduction, antioxidation and the like, and can be used for nutritious food, functional health food, athlete food and the like.

The academy of sciences of Heilongjiang province develops resource innovation of medicinal industrial hemp and new variety breeding on the basis of new variety breeding of fiber, seed and fiber, takes high Cannabidiol (CBD) content as a main selection target, quickly breeds the new variety of the medicinal industrial hemp with high CBD content, and provides a new variety for the development and utilization of industrial hemp in the medical field. In the research, a scientific and rapid breeding technology is combined with standard detection, high CBD content and low THC content are taken as directional selection targets, plants with the CBD content of more than or equal to 1 percent and the THC content of less than 0.3 percent in leaves and inflorescences are selected, and the stability of the genetic character of the variety is determined through the tests of the CBD content and the THC content in at least 4 growth cycles. At present, 2 new medicinal industrial cannabis strains with high CBD content have been bred and screened. The main characteristics of the lines are shown in Table 1

TABLE 1 New strain character of hemp for medical industry with high CBD content bred by Daqing division of department of sciences of Heilongjiang province

Line code	Plant type	Flower color of male flower	Flower color of female flower	CBD content (%)	THC content (%)
						CBD1	Heterosexual plant	Yellow green	Green colour	1.0780	0.0903
CBD2	Heterosexual plant	Yellow green	Green colour	1.0470	0.0918

CBD is nontoxic and harmless, and the well-known health-care product 'first-class macroenemy' is trace toxin contained in the raw materials, so that endless hidden dangers can be caused in the long-term and long-term accumulation. CBD eliminates hidden troubles by scientific extraction of green plants, and the existence and development of CBD from the perspective of medical health care are an irreproducible revolution initiative of human beings in the health field. CBD is a natural component extracted from hemp plants, has extremely high medicinal value, can play a positive role in almost every organ of a human body, can gradually restore the balance of the body and mind, provides mellow, stable and relaxed feeling, and cannot generate any influence on any neurocognitive system. The world health organization has published that cannabidiol CBD is not found in any unhealthy factor, and that naturally occurring CBD, whether used in humans or animals, is safe and does not negatively impact public health. For this reason, the world wide agency for anti-irritants (WADA) has formally removed Cannabidiol (CBD) from the "list of contraband substances" from 1/1 in 2018.

Through search, the inventor searches the patent document of nutrition-enriched honey of CN201010203752.X, the patent document of powder enzyme food prepared from a germinated brown rice of CN201510548276.8, and the three proved formulas of vol.56No.11 of the journal of China bee-keeping in 2005, but the documents do not effectively combine the nutrient substances of the hemp with the honey, and simultaneously have a plurality of problems in the processing process of the hemp, and the problems need to be solved.

Disclosure of Invention

The invention aims to provide a preparation method of hemp honey powder.

The purpose of the invention is realized as follows:

a preparation process of hemp honey powder comprises the following steps:

(1) mixing the ingredient liquid, weighing 10-13 parts of maltodextrin, 1-3 parts of beta-cyclodextrin, 1-3 parts of gelatin and 5-8 parts of fructus cannabis polysaccharide according to parts by weight, adding water, mixing uniformly, adding 80-95 parts of honey, mixing uniformly, adding water to adjust the solid content to 20-30% to obtain a feed liquid; the fructus cannabis polysaccharide is prepared by the following steps:

(1.1) weighing 700g of hemp seeds, peeling, performing microwave treatment for detoxification, and crushing the hemp seeds into powder;

(1.2) soaking in 75% ethanol solution for degreasing, continuously stirring for 35min by using a magnetic stirrer, then centrifuging for 35min by using a centrifugal machine with the rotating speed of 4500r/min, removing supernatant, and collecting precipitate to obtain degreased powder;

(1.3) repeating the step (1.2) for more than four times;

(1.4) extracting the degreased powder by using 800ml of distilled water at room temperature, continuously stirring for 3 hours, centrifuging for 45 minutes by using a centrifugal machine with the rotating speed of 4500r/min, and collecting supernatant;

(1.5) repeating the step (1.4) for more than 3 times, combining all the supernatants, and concentrating under reduced pressure to obtain a fructus cannabis extract concentrated solution;

(1.6) adding a buffer solution and a bacteriostatic agent toluene, adjusting the pH value of the concentrated solution to be between 5.5 and 6, controlling the temperature to be between 36 and 37 ℃, heating to inactivate enzyme, performing 72-hour running water dialysis by using a dialysis bag to remove small molecular impurity pigment, and performing freeze drying on the liquid in the dialysis bag to obtain a dried crude polysaccharide;

(2) homogenizing, namely homogenizing the feed liquid;

(3) emulsifying, adding an emulsifier into the feed liquid, fully stirring to obtain a mixture, and performing high-speed shearing on the mixture by using a shearing machine to emulsify;

(4) spray drying, wherein the emulsified feed liquid is subjected to spray drying, the gas pressure is 0.1MPa, the air inlet temperature is 170-190 ℃, the air outlet temperature is 70-80 ℃, and the feeding amount is 5-8ml/s, so as to obtain the honey powder.

2-3 parts of hyssop extract is added into the blending liquid.

2-5 parts of isomaltulose is added into the blending liquid.

1-2 parts of cymbidium tincture are added into the blending liquid.

The air inlet temperature is 190 ℃, the air outlet temperature is 80 ℃, and the feeding amount is 6 ml/s.

The air inlet temperature is 170 ℃, and the air outlet temperature is 8 ml/s.

The emulsifier is one or more of modified starch, soybean lecithin, sodium caseinate, sucrose ester or whey protein.

The shearing emulsification adopts a shearing emulsifying machine, the emulsifying time is 3-5min, and the emulsifying speed is 9000-11000 r/min.

And carrying out two-stage homogenization by using a high-pressure homogenizer, wherein the first homogenization pressure is 40-50Mpa, and the second homogenization pressure is 20-30 Mpa.

The invention has the beneficial effects that:

the hemp powder can supplement the protein content which is lacked by honey, so that the honey is richer in nutrition, and the hemp protein is closer to human protein, is easy to absorb and has no anaphylactic reaction. The honey is added with the nutritive value, the polysaccharide contained in the product has various effects of resisting cancer, resisting inflammation, protecting cardiovascular system, improving body immunity and the like, and especially the hemp polysaccharide also has the effects of relaxing bowel, expelling toxin and the like. The maltodextrin, the beta-cyclodextrin, the gelatin, the hemp gum and the hemp polysaccharide added in the feed liquid have good solubility and low hygroscopicity, and play a role in reducing the hygroscopicity of the honey powder. The beta-cyclodextrin is white or almost white crystal fixed or crystalline powder, a hydrophobic cavity is arranged in a molecule, the beta-cyclodextrin can form a complex with organic molecules, can embed heat-sensitive components and aroma-producing substances in honey, protects the heat-sensitive substances and the aroma-producing substances in spray, and has less loss of the prepared honey powder heat-sensitive substances and more retention of honey aroma. After the hemp seeds are extracted to obtain the polysaccharide, the residual seed meal is milled, so that the utilization rate of the hemp seeds is improved.

The invention has high content of CBD component and good health care function; the hemp honey powder prepared by the method can promote the effective components of the hemp seeds to be efficiently absorbed without adding other stabilizers; the hemp seed powder is added into the honey, so that the protein content can be increased, the nutrient composition ratio in the honey can be improved, the content of omega-3 polyunsaturated fatty acid can be increased, the proportion of omega-3 and omega-6 is more reasonable, and the sesame seed powder can be absorbed by a human body more easily. The method adopts warm water to pretreat the hemp seeds, and is beneficial to making the particles of the refined powder from the hemp seeds more uniform. The polyunsaturated fatty acid and dietary fiber in fructus Cannabis can effectively delay aging, prevent cardiovascular diseases, promote brain development, resist inflammation and cancer, reduce blood viscosity, maintain heart activity and brain thinking agility, and keep digestive tract unobstructed and healthy.

Drawings

FIG. 1 is a flow chart of the method of the present invention;

FIG. 2 is a comparison of activity of solid alkaline protease with 1,2,3,4, 5-pentanol protectant added at different temperatures;

FIG. 3 is a comparison of activity of solid alkaline protease at different temperatures;

FIG. 4 is a comparison of activity of liquid alkaline protease at different temperatures;

FIG. 5 is a comparison of activity of solid alkaline protease with 1,2,3,4, 5-pentanol protectant added at different temperatures;

FIG. 6 is a graph showing the decolorization effect of the hemp clear solution.

Detailed Description

The invention is further described below with reference to the accompanying drawings.

The invention provides a preparation method of hemp honey powder, which comprises the following basic steps:

a preparation process of hemp honey powder comprises the following steps:

the method comprises the following steps: preparation of fructus Cannabis polysaccharide

Weighing fructus Cannabis, peeling, crushing into powder, soaking in ethanol solution for defatting, stirring with magnetic stirrer, centrifuging with centrifuge, removing supernatant, and collecting precipitate to obtain defatted powder. The above process was performed 4 times in sequence. Extracting the defatted powder with distilled water at room temperature, stirring, centrifuging with centrifuge, collecting supernatant, collecting precipitate, further performing drawer extraction with distilled water at room temperature, mixing the supernatants, and concentrating under reduced pressure to obtain concentrated solution of Cannabis sativa seed extract.

The pH value of the concentrated solution is adjusted by using a buffer solution and a bacteriostatic agent toluene, the temperature is controlled to be 36-37 ℃, namely the body temperature of animals, because the activity of pepsin under the condition is the maximum, the main purpose of the process is to fully decompose protein macromolecules into micromolecules such as amino acid and the like, and keep macromolecular polysaccharide. Heating to inactivate enzyme, dialyzing with running water in dialysis bag to remove small molecular impurity pigment, and freeze drying the solution in dialysis bag to obtain crude polysaccharide.

Step two: homogenizing, namely homogenizing the feed liquid under the homogenizing pressure of 20 MPa;

step three: spray drying, wherein the homogenized material liquid is subjected to spray drying, the gas pressure is 0.1MPa, the air inlet temperature is 170-190 ℃, the air outlet temperature is 70-80 ℃, and the feeding amount is 5-8ml/s, so as to obtain the honey powder.

The blending liquid is prepared by weighing 10-12 parts of maltodextrin, 1-2 parts of beta-cyclodextrin, 1-2 parts of gelatin and 5-8 parts of fructus cannabis polysaccharide by weight parts, adding water, mixing uniformly, adding 80-95 parts of honey, mixing uniformly, adding water to adjust the solid content to 20-25%, and obtaining the feed liquid.

In the homogenizing process, the temperature of the feed liquid is controlled at 30-40 ℃, and the feed liquid is homogenized under the homogenizing pressure of 20 MPa.

The air inlet temperature is 190 ℃, the air outlet temperature is 80 ℃, and the feeding amount is 6 ml/s; or the air inlet temperature is 170 ℃ and the air outlet temperature is 8 ml/s.

The following examples are given below in conjunction with the technical solutions of the present invention.