阅读说明：本技术 一种从白刺果干中提取氨基酸的方法 (Method for extracting amino acid from dried nitraria tangutorum bobr ) 是由 袁增辉 马烨 李冬冬 赵婌君 于 2019-10-10 设计创作，主要内容包括：本发明公开了一种从白刺果干中提取氨基酸的方法,将白刺果干清洗后晾干；置于粉碎机中粉碎后,加入一定量的水,超声处理一段时间；再加入一定量的乙醇,浸泡醇提一段时间后,过滤,取滤液；再经减压抽滤滤液,除去溶剂后,制得氨基酸。本发明以醇提法,并辅以利用超声波作用于粉碎后的白刺果,通过空化现象提取细胞中的有效成分,并提高有效成分的提出率。其操作步骤简单易行,过程无污染；通过选取最合适的温度与时间,提高醇提效率,既提高了产率,又保证了产出的总氨基的活性；减压抽滤的方式,有效防止高温蒸馏溶剂对活性成分的影响,为白刺果的综合利用和深度开发提供了新的方向,具有很强的实用性和广泛的适用性。(The invention discloses a method for extracting amino acid from nitraria tangutorum bobr, which is characterized in that nitraria tangutorum bobr is cleaned and dried in the air; placing the mixture into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time; adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, filtering, and collecting filtrate; and then carrying out vacuum filtration on the filtrate, and removing the solvent to obtain the amino acid. The invention uses alcohol extraction method and uses ultrasonic wave to act on the crushed nitraria tangutorum bobr, extracts effective components in cells through cavitation phenomenon, and improves the extraction rate of the effective components. The operation steps are simple and easy, and the process is pollution-free; by selecting the most appropriate temperature and time, the alcohol extraction efficiency is improved, the yield is improved, and the activity of the produced total amino is ensured; the reduced pressure filtration mode effectively prevents the influence of high temperature distillation solvent on active ingredients, provides a new direction for the comprehensive utilization and the deep development of the nitraria tangutorum bobr, and has strong practicability and wide applicability.)

1. A method for extracting amino acid from a dried nitraria tangutorum bobr is characterized by comprising the following steps:

s1, cleaning and airing the dried nitraria tangutorum bobr;

s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time;

s3, adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, and filtering to obtain a filtrate;

s4, filtering the filtrate under reduced pressure to obtain the amino acid.

2. The method of claim 1, wherein the washing in step S1 comprises washing with running water and then with deionized water;

and drying, including draining with gauze and drying.

3. The method for extracting amino acid from the dried Nitraria tangutorum bobr according to claim 1, wherein the ultrasonic treatment in the step S2 is performed at a power of 90-120w for 1-2 h.

4. The method for extracting amino acid from the dried nitraria tangutorum bobr according to claim 1, wherein the ethanol in the step S3 is 15-25 times of the mass of the dried nitraria tangutorum bobr, and the concentration of the ethanol is 60 wt%.

5. The method for extracting amino acid from the dried nitraria tangutorum bobr according to claim 1, wherein the alcohol extraction in the step S3 is performed at 60-70 ℃ for 1.5-2 h.

Technical Field

The invention relates to an extraction method of amino acid, in particular to a method for extracting amino acid from dried nitraria tangutorum bobr.

Background

The nitraria tangutorum bobr belongs to the genus nitraria and the family Zygophyllaceae, and is a multi-branch saline-alkali-resistant droughty type short shrub. Nitraria sibirica pall is usually grown in salinization areas in desert regions and has the physiological characteristics of drought resistance, high temperature resistance, strong water retention capacity and the like. The white thorns are excellent sand-fixing shrub plants in the sand areas of China and are important research objects in wind and sand protection. The fruits are oval like soybean grains, are dark red or orange yellow after being ripened, can be directly eaten, are called desert cherries and are called third-class fruits.

The nitraria tangutorum bobr has rich resources and wide distribution, and is wild in the places of Zhangjiakou, Tianjin, Cangzhou and the like in the northwest desert area of China and the coastal areas of North China and northeast China. According to statistics, the nitraria tangutorum bobr basins are distributed in pollution-free areas with the altitude of more than 3000 m, 6-10 ten thousand tons of nitraria tangutorum bobr can be produced every year, and the method has great development value. There are records as early as in compendium of materia Medica: bai Ci has pungent, cold and nontoxic flavor, and can treat swelling and abscess, promote pus discharge, tonify kidney qi and replenish essence and marrow. But the people really began to pay attention to the nutritional value of the nitraria tangutorum bobr after the 90 s of the 20 th century. Researches show that the content of crude protein in the nitraria tangutorum bobr is 10.6-14.4%, the content of fat and fruit oil in pulp is 3.5%, the content of amino acid C also reaches 7.8-18.3%, more importantly, the nitraria tangutorum bobr also contains abundant nutrient substances such as protein, fat, sugar and the like, and the content of flavone is up to 1.1-1.9%.

The nitraria tangutorum bobr has good medicinal and edible values, is sour, sweet and delicious, has the effects of nourishing the stomach, tonifying the spleen, relieving exterior syndrome, soothing the nerves, nourishing yin, moistening the lung, promoting lactation and the like, and also has certain curative effects on weakness, irregular menstruation and dyspepsia.

Disclosure of Invention

In order to solve the defects of the prior art, the invention aims to provide a method for extracting amino acid from the dried nitraria tangutorum bobr with high efficiency and low cost.

In order to achieve the above object, the present invention adopts the following technical solutions:

a method for extracting amino acid from a dried nitraria tangutorum bobr comprises the following steps:

s1, cleaning and airing the nitraria tangutorum bobr;

s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time;

s3, adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, and filtering to obtain a filtrate;

s4, filtering the filtrate under reduced pressure, and removing the solvent to obtain the amino acid.

The cleaning in the step S1 includes washing with running water, and then washing with deionized water; and drying, including draining with gauze and drying.

And (5) carrying out ultrasonic treatment in the step S2, wherein the power is 90-120w, and the time is 1-2 h. Preferably, the power is 120w and the time is 1 h.

The ethanol in the step S3 is 15-25 times of the dry mass of the nitraria tangutorum bobr, and the concentration of the ethanol is 60 wt%. Preferably 20 times the dry mass of the nitraria tangutorum bobr.

The alcohol extraction in the step S3 is carried out at the temperature of 60-70 ℃ for 1.5-2 h. The temperature is preferably 65 ℃.

The invention has the advantages that:

the invention relates to a method for extracting amino acid from nitraria tangutorum bobr, which utilizes ultrasonic waves to act on crushed nitraria tangutorum bobr, and then adopts an alcohol extraction method to extract total amino acid from the nitraria tangutorum bobr; when ultrasonic waves act on the medium (the white thorn), the medium is torn into a plurality of small cavities, the small cavities are closed instantly, and instantaneous pressure of thousands of atmospheres, namely cavitation, is generated when the small cavities are closed. The cell wall is broken up and decomposed due to the extremely high pressure generated in the cavitation process, so that the solvent can penetrate into the cell, components in the cell are dissolved in the solvent, and the components are dissolved in the solvent under the action of ultrasonic waves, so that the extraction rate of effective components is improved.

The method adopts an alcohol extraction method, takes ethanol as an extracting solution to extract the amino acid in the nitraria tangutorum bobr, has simple and easy operation steps, no pollution in the process, low alcohol extraction temperature, short time, high efficiency and low cost, improves the yield, and ensures the activity of the obtained total amino; the reduced pressure filtration mode effectively prevents the influence of high temperature distillation solvent on active ingredients, provides a new direction for the comprehensive utilization and the deep development of the nitraria tangutorum bobr, and has strong practicability and wide applicability.

Detailed Description

The present invention will be described in detail with reference to the following embodiments.

A method for extracting vitamins from a nitraria tangutorum bobr dry fruit comprises the following steps: the white thorn fruit intervention treatment (S1) → ultrasonication (S2) → alcohol extraction (S3) → vacuum filtration under reduced pressure (S4) → finished product.

S1, washing the bur fruits with flowing water to remove floating dust, washing with deionized water, and draining with gauze to dry for later use;

s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer, pulverizing for 30-50min at the low temperature at the rotating speed of 2000-3500r/min, adding water with the same volume, and carrying out ultrasonic treatment for 1-2h at the power of 90-120w for later use;

s3, adding 60 wt% ethanol 15-25 times of the weight of the nitraria tangutorum bobr dry, soaking and extracting the mixture with ethanol at 60-70 ℃ for 1.5-2 h, and filtering to obtain filtrate; standby;

s4, filtering the filtrate obtained by alcohol extraction under reduced pressure, and removing the solvent to obtain the amino acid.