阅读说明：本技术 一种菌剂组合物及其应用 (Bacterium agent composition and application thereof ) 是由 陈林 王溢 于 2019-11-08 设计创作，主要内容包括：本发明公开了一种复合菌剂颗粒,其配方如下所示：瑞士乳杆菌菌粉(5-10重量份)、3,4-二羟基苯甲酸-3-O-α-L-鼠李糖苷(3-5重量份)、硬脂酸镁(1-2重量份)、无水磷酸三钠(2-3重量份)、羟丙甲基纤维素(5-10重量份)、黄原胶(1-3重量份)、蔗糖(200重量份)。(The invention discloses a composite microbial inoculum particle, which has the following formula: lactobacillus helveticus bacterium powder (5-10 parts by weight), 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside (3-5 parts by weight), magnesium stearate (1-2 parts by weight), anhydrous trisodium phosphate (2-3 parts by weight), hydroxypropyl methyl cellulose (5-10 parts by weight), xanthan gum (1-3 parts by weight) and sucrose (200 parts by weight).)

1. A composite microbial agent particle, comprising:

lactobacillus helveticus, 3, 4-dihydroxybenzoic acid-3-O- α -L-rhamnoside.

2. The composite microbial agent particle as claimed in claim 1, wherein the lactobacillus helveticus is lactobacillus helveticus NS 8.

3. The composite microbial inoculant particle of claim 2, wherein lactobacillus helveticus NS8 culture method: inoculating a ring of lactic acid bacteria into an MRS solid culture medium, streaking, and carrying out anaerobic culture at 37 ℃ for 24 h;

selecting single colony with better growth in the plate, inoculating the single colony into MRS liquid culture medium for activation, and anaerobically culturing to 5 x 10⁷(CFU/ml) lactobacillus helveticus NS8 seed fluid;

gradually amplifying the seed liquid of the lactobacillus helveticus NS8 to a large tank for fermentation, centrifuging, and freeze-drying to obtain the bacterial powder.

4. The composite microbial agent particle according to any one of claims 1 to 3, which comprises the following specific components:

lactobacillus helveticus bacterium powder (5-10 parts by weight), 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside (3-5 parts by weight), magnesium stearate (1-2 parts by weight), anhydrous trisodium phosphate (2-3 parts by weight), hydroxypropyl methyl cellulose (5-10 parts by weight), xanthan gum (1-3 parts by weight) and sucrose (200 parts by weight).

5. The method for preparing composite bacterial agent particles as claimed in claim 4, comprising the steps of:

(1) preparing materials, namely sieving various materials by a 40-mesh sieve for later use;

(2) sugar particle preparation: adding sucrose (200 parts by weight) and xanthan gum (1-3 parts by weight) into a horizontal mixer, mixing for 15 minutes, adding purified water, stirring, placing on a swing granulator, sieving with a 30-mesh sieve, granulating, drying wet granules in a dryer, and controlling the actual temperature to be 40-60 ℃; sieving the dried granules with a 30-mesh sieve;

the water content should not exceed 1.5%;

(3) preparing composite microbial inoculum particles: putting lactobacillus helveticus bacterium powder (5-10 parts by weight), 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside (3-5 parts by weight) and hydroxypropyl methyl cellulose (5-10 parts by weight) into a horizontal mixer, starting stirring, uniformly mixing, adding a proper amount of water, stirring for 30min, sieving particles with a 30-mesh sieve, and drying at 35-40 ℃ for 30 min;

(4) total mixing: mixing the composite microbial inoculum particles, magnesium stearate (1-2 parts by weight) and anhydrous trisodium phosphate (2-3 parts by weight) for 30-60 minutes.

6. A functional dairy product comprising the composite microbial inoculant particles of claims 1-5.

Technical Field

The invention relates to a microbial inoculum composition and application thereof, in particular to a microbial inoculum composition for conditioning anorexia.

Background

The human body inhabits numerous microorganisms, with the micro-ecosystem being the most dominant, most complex system. When the immunity of a human is reduced, probiotics play an important role in human health. This view has been confirmed in sterile rodents and birds. Microbial colonization plays an important role in nutrition, in vivo growth, induction and regulation of immune and endocrine balance, maturation of the central nervous system and behavior. At present, the largest ecological site of the microbial community is the intestinal tract of human beings, and the structure and diversity of the intestinal microecology play a vital role in the health of human beings and animals. Bacteria of the stomach are also very important. The pH value of gastric acid is low, bacteria can reach the intestinal tract through the stomach, the intestinal tract can play a role in absorption, and when the microecological structure or diversity of the gastrointestinal tract is disordered, various diseases can be caused.

Anorexia is a long-term loss of appetite and appetite caused by various reasons, and then gradually causes emaciation, slow development of intelligence and reduced immunity, is a disease which is easy to cause various diseases and is a common disease and frequently encountered disease. According to the traditional Chinese medicine, the pathological viscera mainly comprise spleen and stomach, the pathogenesis of the pathological viscera is that the spleen fails to transport and transform, the spleen and the stomach are mutually exterior and interior, the spleen governs transportation and transformation, the stomach governs accepting, the spleen fails to harmonize with the stomach, and the stomach fails to eat. Therefore, in terms of treatment, the basic principle of regulating the spleen and stomach is mostly adopted, and the microorganisms have important biological and medical significance for the experiments of gastric emptying and small intestine propulsion and the discussion of the improvement of the digestion function.

The invention aims to provide composite microbial inoculum particles effective on anorexia.

Disclosure of Invention

The invention aims to provide composite microbial inoculum particles effective for anorexia.

Lactobacillus helveticus (Lactobacillus helveticus), the first isolate of Lactobacillus species, 0.6-1.0X2.0-6.0 microns, single or chained, stained with methylene blue without heterochromogens(this is different from Lactobacillus delbrueckii, Lactobacillus bulgaricus, Lactobacillus reicheri and Lactobacillus lactis.) on agar plates, the colony diameter is 2 ~ 3mm or less, and is generally opaque, white to light gray, rough to rhizoid, anaerobic and 5% CO₂The growth of yacon streak culture under the condition is greatly increased, and the colony is larger or smoother in a culture medium containing Tween 80 or sodium oleate.

Lactobacillus helveticus (Lactobacillus helveticus) NS8 selected in the embodiments of the present invention was isolated from Koumiss in the region of sulindac, and was purchased as a commercially available product.

The Asian peltate yam rhizome (AspidopterysobcordiataHemsl.) is a woody vine of the genus Astrocarya of the family Saxifragaceae, and is mainly distributed in shrubs at forest edges of high-altitude mountainous regions in Hainan, Yunnan and other regions of China. The number of the plants is 20 in the world, and about 8 in China, wherein two kinds of plants are used for medicine. Leaves of Guizhou guinii (a. calvalerieilevl.) are used as a medicine for treating infantile malnutrition. The Peucedanum comprises stems of various Gymnema Yunnanense Tsiang, radix Stephaniae Sinicae is used as medicine, has astringent and cool properties, has the effects of diminishing inflammation, promoting urination, clearing heat and removing urinary calculus, and is mainly used for treating urinary tract infection, cystitis, lithangiuria, rheumatic osteodynia, postpartum asthenia, inappetence and other diseases.

Raja et al have a planar structure of 1 new rhamnoside (Chinese Herbal medicine Traditional and Herbal Drugs, 50, 5, 2019, 3 months) from obdural sinensis, wherein the rhamnoside is as follows: 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside.

The inventor finds that the 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside and Lactobacillus helveticus NS8 have a synergistic effect of improving anorexia.

The technical problem to be solved by the invention can be realized by the following technical scheme.

A composite microbial agent particle, comprising:

lactobacillus helveticus, 3, 4-dihydroxybenzoic acid-3-O- α -L-rhamnoside.

Preferably, the lactobacillus helveticus is lactobacillus helveticus NS 8. Lactobacillus helveticus (Lactobacillus helveticus) NS8 was isolated from Koumiss in the region of Silileo, isolated, identified and stored in the psycho-health stress laboratory behavior biology laboratory of the academy of sciences of China, and was a commercially available product.

The culture method of the lactobacillus helveticus NS8 comprises the following steps: inoculating a ring of lactobacillus to MRS solid culture medium, streaking, and performing anaerobic culture at 37 ℃ for 24 h. Selecting single colony with better growth in the plate, inoculating the single colony into MRS liquid culture medium for activation, and anaerobically culturing to 5 x 10⁷(CFU/ml) Lactobacillus helveticus NS8 seed fluid. Gradually amplifying the seed liquid of the lactobacillus helveticus NS8 to a large tank for fermentation, centrifuging, and freeze-drying to obtain the bacterial powder.

The 3, 4-dihydroxy-benzoic acid-3-O-alpha-L-rhamnoside is obtained by separating and extracting the echinocandis sinensis from the method of the reference (1 new rhamnoside in the echinocandis sinensis, Chinese Herbal medicine Chinese Traditional and Herbal Drugs volume 50, 3 months 2019).

The formula of the composite microbial inoculum particle is as follows:

lactobacillus helveticus bacterium powder (5-10 parts by weight), 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside (3-5 parts by weight), magnesium stearate (1-2 parts by weight), anhydrous trisodium phosphate (2-3 parts by weight), hydroxypropyl methyl cellulose (5-10 parts by weight), xanthan gum (1-3 parts by weight) and sucrose (200 parts by weight).

The preparation method comprises the following steps:

(1) preparing materials, namely screening various materials through a 40-mesh sieve for later use.

(2) Sugar particle preparation: adding sucrose (200 parts by weight) and xanthan gum (1-3 parts by weight) into a horizontal mixer, mixing for 15 minutes, adding purified water, stirring, placing on a swing granulator, sieving with a 30-mesh sieve, granulating, drying wet granules in a dryer, and controlling the actual temperature to be 40-60 ℃; the dried granules were sieved through a 30 mesh sieve. The moisture should not exceed 1.5%.

(3) Preparing composite microbial inoculum particles: putting lactobacillus helveticus bacterium powder (5-10 parts by weight), 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside (3-5 parts by weight) and hydroxypropyl methyl cellulose (5-10 parts by weight) into a horizontal mixer, stirring, mixing uniformly, adding a proper amount of water, stirring for 30min, sieving with a 30-mesh sieve, grading, and drying at 35-40 ℃ for 30 min.

(4) Total mixing: mixing the composite microbial inoculum particles, magnesium stearate (1-2 parts by weight) and anhydrous trisodium phosphate (2-3 parts by weight) for 30-60 minutes.

The invention has the advantages that:

the inventor finds that the 3, 4-dihydroxy-benzoic acid-3-O-alpha-L-rhamnoside can be synergistically acted on the stomach with Lactobacillus helveticus (Lactobacillus helveticus) NS8, and has the effect of synergistically improving anorexia.

Detailed Description

The following examples of the present invention are described in detail, and are only for the purpose of illustrating the present invention and are not to be construed as limiting the present invention.

Specific examples of the present invention are described below.

Preparation of lactobacillus helveticus NS8 bacterial powder:

the culture method of the lactobacillus helveticus NS8 comprises the following steps: inoculating a ring of lactobacillus to MRS solid culture medium, streaking, and performing anaerobic culture at 37 ℃ for 24 h. Selecting single colony with better growth in the plate, inoculating the single colony into MRS liquid culture medium for activation, and anaerobically culturing to 5 x 10⁷(CFU/ml) Lactobacillus helveticus NS8 seed fluid. Gradually amplifying the seed liquid of the lactobacillus helveticus NS8 to a large tank for fermentation, centrifuging, and freeze-drying to obtain the bacterial powder.

Preparing 3, 4-dihydroxy benzoic acid-3-O-alpha-L-rhamnoside:

the 3, 4-dihydroxy-benzoic acid-3-O-alpha-L-rhamnoside is obtained by separating and extracting the echinocandis sinensis from the method of the reference (1 new rhamnoside in the echinocandis sinensis, Chinese Herbal medicine Chinese Traditional and Herbal Drugs volume 50, 3 months 2019).