阅读说明：本技术 一种副干酪乳杆菌n1115改善糖尿病的应用及相应的奶粉、应用 (Application of lactobacillus paracasei N1115 to improvement of diabetes, corresponding milk powder and application ) 是由 张栋 王海波 王世杰 冯丽莉 薛玉玲 荀一萍 朱宏 封肖颖 于 2019-09-23 设计创作，主要内容包括：本发明提供了一种副干酪乳杆菌N1115改善糖尿病的应用及相应的奶粉、应用,其中,所述副干酪乳杆菌N1115用于预防或缓解糖尿病；而利用副干酪乳杆菌N1115制备的奶粉不仅能够有效预防或缓解糖尿病,同时配方合理,营养丰富。(The invention provides an application of lactobacillus paracasei N1115 for improving diabetes, corresponding milk powder and an application, wherein the lactobacillus paracasei N1115 is used for preventing or relieving the diabetes; the milk powder prepared by using the lactobacillus paracasei N1115 can effectively prevent or relieve diabetes, and is reasonable in formula and rich in nutrition.)

1. The application of lactobacillus paracasei N1115 for improving diabetes is characterized in that: the lactobacillus paracasei N1115 is used for preventing or relieving diabetes.

2. The milk powder for improving the diabetes is characterized by comprising the following raw materials in parts by weight: 40-45 parts of raw milk powder, 28-33 parts of desalted whey powder, 5-8 parts of white granulated sugar, 4-4.5 parts of anhydrous cream, 3-4 parts of soybean oil, 3-4 parts of lactose, 2-4 parts of concentrated whey protein powder, 1.5-2 parts of galacto-oligosaccharide, 1-1.5 parts of fructo-oligosaccharide, 0.5-1 part of phospholipid, 0.1-0.2 part of DHA algal oil powder, 0.1-0.3 part of arachidonic acid powder, 0.05-0.2 part of choline chloride, 0.1-0.8 part of calcium carbonate, 0.02-0.06 part of nucleotide and 0.1 part of compound vitamin0.5 part, 0.1-0.3 part of composite mineral substance and 0.1-1 part of probiotic powder capable of improving diabetes; wherein the content of Lactobacillus paracasei N1115 in the probiotic powder capable of improving diabetes is 1 × 10⁸CFU/g-9×10¹⁰CFU/g。

3. The use of milk powder for improving diabetes as claimed in claim 2, wherein: the milk powder for improving diabetes is used for preventing or relieving diabetes.

Technical Field

The invention belongs to the field of bioengineering, and relates to an application of a strain for improving diabetes, in particular to an application of lactobacillus paracasei N1115 for improving diabetes; the invention also provides milk powder prepared by using the lactobacillus paracasei N1115 for improving diabetes and application thereof.

Background

Diabetes is a chronic metabolic disease caused by genetic factors, environmental factors and living habits. The main reasons are a syndrome of elevated blood glucose levels as a result of the inability of the human pancreas to produce insulin normally (insulin deficiency) or the body to utilize insulin normally (insulin resistance). Hyperglycemia is caused by a defect in insulin secretion or an impaired biological action, or both. Diabetes is a long-standing hyperglycemic condition that can lead to chronic damage to, and dysfunction of, various tissues, particularly the eye, kidneys, heart, blood vessels, nerves. If the disease condition is not well controlled, complications such as heart disease, coronary heart disease, cerebrovascular disease, retinal vascular disease, renal arteriosclerosis, limb arteriosclerosis and the like can be caused in the future. According to the latest data released by the national institutes of health on day 11/12 in 2016, the following are shown: the total number of Chinese diabetics reaches 1 hundred million and will continue to increase, and China now becomes the world with the largest number of diabetics. How to prevent diabetes in advance becomes a current problem.

Research shows that probiotics has preventive, relieving and therapeutic effects on diabetes, and potential intervention mechanisms of the probiotics comprise:

1 Activity for inhibiting alpha-glucosidase

Alpha-glucosidase is a key enzyme in the intestinal lumen and brush border membrane that promotes carbohydrate hydrolysis, which degrades starch and polysaccharides into absorbable monosaccharides. Inhibiting the activity of alpha-glucosidase, reducing the absorption of glucose and lowering the postprandial blood glucose level.

2 regulating intestinal flora

Reports indicate that the intestinal flora of diabetics is unbalanced, but probiotics can colonize the intestinal tract, regulate the intestinal flora and increase the expression of adhesion proteins, and form a natural biological barrier of the intestinal tract together with intestinal mucosa. The barrier can reduce the number of harmful bacteria in the intestinal tract, increase the number of beneficial bacteria, and maintain the balance of intestinal flora, thereby reducing the content of lipopolysaccharide in endotoxin and the permeability of epithelial cells in the intestinal tract, reducing the release of inflammatory factors and oxidative stress reaction, and further reducing the damage to beta cells of pancreatic islets.

3 antioxidation

Diabetes is often accompanied by oxidative stress, i.e.: excessive production of free radicals and destruction of the antioxidant defense system. This will lead to cellular and enzymatic damage, increased lipid peroxidation, and development of insulin resistance. It also destroys islet beta cells leading to diabetes. The antioxidant action of the strain has been proved by research, and the antioxidant mechanism can be divided into active oxygen scavenging, metal ion chelating, activity of inhibiting pro-oxidase, reduction activity and oxidation of ascorbic acid inhibiting.

4 regulating immunity and reducing inflammation

Diabetes is often associated with long-term low-grade inflammation that can lead to diabetes by disrupting endothelial cell structure and function, causing a barrier to insulin transport in tissue cells, and leading to islet beta cell structure and dysfunction. The probiotics can regulate the immune function of the organism by adhering epithelial cells, and reduce inflammation by regulating the secretion of anti-inflammatory factors, thereby preventing and controlling the occurrence of diabetes.

However, no product for preventing or relieving diabetes by using probiotics exists in the market at present, so that the market is blank at present.

Disclosure of Invention

The invention aims to solve the technical problem of providing an application of lactobacillus paracasei N1115 in improving diabetes, and the lactobacillus paracasei N1115 is used for preventing or relieving diabetes. The lactobacillus paracasei N1115 is separated and screened from traditional fermented milk products in inner Mongolia of China, is disclosed in patent CN201110357058.8, has been preserved in the strain collection center of the institute of microbiology of Chinese academy of sciences in 2011 for 3 months, and has the preservation number of CGMCC 4691.

The invention also provides milk powder for improving diabetes, which is prepared by adding lactobacillus paracasei N1115 into the raw materials, and the milk powder can effectively prevent or relieve diabetes.

The invention also provides an application of the milk powder for improving diabetes, which is used for preventing or relieving diabetes.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the application of lactobacillus paracasei N1115 in improving diabetes, wherein the lactobacillus paracasei N1115 is used for preventing or relieving diabetes.

The invention also provides milk powder for improving diabetes, which is prepared from the following raw materials in parts by weight: 40-45 parts of raw milk powder, 28-33 parts of desalted whey powder, 5-8 parts of white granulated sugar, 4-4.5 parts of anhydrous cream, 3-4 parts of soybean oil, 3-4 parts of lactose, 2-4 parts of concentrated whey protein powder, 1.5-2 parts of galacto-oligosaccharide, 1-1.5 parts of fructo-oligosaccharide, 0.5-1 part of phospholipid, 0.1-0.2 part of DHA algal oil powder, 0.1-0.3 part of arachidonic acid powder, 0.05-0.2 part of choline chloride, 0.1-0.8 part of calcium carbonate, 0.02-0.06 part of nucleotide, 0.1-0.5 part of compound vitamin, 0.1-0.3 part of compound mineral substance and 0.1-1 part of probiotic powder capable of improving diabetes; wherein the content of Lactobacillus paracasei N1115 in the probiotic powder capable of improving diabetes is 1 × 10⁸CFU/g-9×10¹⁰CFU/g。

The invention also provides an application of the milk powder for improving diabetes, which is used for preventing or relieving diabetes.

Compared with the prior art, the invention has the following advantages:

1. The lactobacillus paracasei N1115 can prevent or relieve diabetes.

2. The lactobacillus paracasei N1115 can regulate immunity, reduce the damage of oxidation to insulin secreting cells, increase intestinal barrier function and reduce the occurrence of long-term low-grade inflammation.

3. The lactobacillus paracasei N1115 can improve the symptoms of thirst and polydipsia by improving the diabetic milk powder.

Description of the drawings:

FIG. 1 is a graph showing the variation of the N1115 TEER value in the evaluation of intestinal barrier function in example 1 of the present invention.

FIG. 2 is a graph showing the change in LGG TEER values in the evaluation of intestinal barrier function in example 1 of the present invention.

FIG. 3 shows the hydroxyl radical scavenging ability of probiotic bacteria in the evaluation of the anti-oxidative ability according to example 1 of the present invention.

FIG. 4 is a graph showing the ability of probiotic bacteria to scavenge superoxide anions in the evaluation of the anti-oxidative ability in example 1 of the present invention.

Fig. 5 is a graph of the DPPH scavenging ability of probiotic bacteria in the evaluation of the anti-oxidative ability according to example 1 of the present invention.

FIG. 6 shows the immunomodulatory capacity of active N1115 in the assessment of immunomodulatory capacity in example 1 of the present invention.

FIG. 7 is a graph showing the immunoregulatory ability of heat-inactivated N1115 in the evaluation of the immunoregulatory ability in example 1 of the present invention.

Detailed Description

The present invention is described in further detail below with reference to specific examples, which are intended to be illustrative only and not limiting.