阅读说明：本技术 一种促进乳酸菌多糖合成量增加的培养基及方法 (Culture medium and method for promoting increase of synthesis amount of lactobacillus polysaccharide ) 是由 黄先敏 杨永吉 蔺应理 陈宇 何天鹏 于 2019-10-14 设计创作，主要内容包括：本发明公开了一种促进乳酸菌多糖合成量增加的培养基及方法,该方法包含：采用的培养基包含以下重量份数的成分：鲜牛奶65～75份,白砂糖4～7份,天麻提取液15～19份；在所述培养基上接种乳酸菌菌种进行发酵,可促进乳酸菌多糖合成量的增加。本发明的方法通过采用含有天麻的培养基,可使乳酸菌在生长代谢中多糖的产量增加。(The invention discloses a culture medium and a method for promoting the increase of the synthesis amount of lactobacillus polysaccharide, wherein the method comprises the following steps: the adopted culture medium comprises the following components in parts by weight: 65-75 parts of fresh milk, 4-7 parts of white granulated sugar and 15-19 parts of a gastrodia elata extract; the culture medium is inoculated with lactobacillus strains for fermentation, which can promote the increase of the synthesis amount of lactobacillus polysaccharide. The method of the invention can increase the yield of the polysaccharide in the growth and metabolism of the lactic acid bacteria by adopting the culture medium containing the gastrodia elata.)

1. A method for increasing the amount of a polysaccharide synthesized by a lactic acid bacterium, the method comprising:

The adopted culture medium comprises the following components in parts by weight: 65-75 parts of milk, 4-7 parts of white granulated sugar and 15-19 parts of a gastrodia elata extract;

And inoculating lactobacillus strains on the culture medium for fermentation to promote the increase of the synthesis amount of lactobacillus polysaccharide.

2. The method of increasing the amount of polysaccharide synthesized by lactic acid bacteria according to claim 1, wherein the lactic acid bacteria species comprises: streptococcus thermophilus and/or Lactobacillus bulgaricus.

3. The method for promoting the increase of the synthesis amount of the lactic acid bacteria polysaccharides as claimed in claim 1, wherein the weight ratio of the gastrodia elata extract is 1: 10 is obtained by extracting rhizoma Gastrodiae powder with water.

4. The method for promoting increase of synthesis amount of lactobacillus polysaccharide according to claim 3, wherein the rhizoma Gastrodiae powder is sheared and stirred with water at 10000rpm, and the supernatant obtained by centrifuging the mixed solution is rhizoma Gastrodiae extractive solution.

5. The method for promoting the increase of the synthesis amount of lactic acid bacteria polysaccharides according to claim 4, wherein the centrifugal rotation speed is 8800 r/min.

6. The method for promoting the increase of the synthesis amount of the lactic acid bacteria polysaccharides as claimed in claim 3, wherein the powder of the gastrodia elata is obtained by crushing tubers of the gastrodia elata and then sieving the crushed tubers with a 180-mesh sieve.

7. The method for promoting the increase of the synthesis amount of a lactic acid bacterial polysaccharide according to any one of claims 1 to 5, wherein the fermentation is performed at 38 to 43 ℃.

8. A culture medium for promoting the increase of the synthesis amount of lactobacillus polysaccharide is characterized by comprising the following components in parts by weight: 65-75 parts of milk, 4-7 parts of white granulated sugar and 15-19 parts of a gastrodia elata extract.

9. The culture medium for promoting increase of synthesis amount of lactic acid bacteria polysaccharides according to claim 8, wherein the weight ratio of the gastrodia elata extract is 1: 10 is obtained by extracting rhizoma Gastrodiae powder with water.

10. a new use of rhizoma Gastrodiae is provided, wherein the rhizoma Gastrodiae is used for promoting the increase of synthesis amount of lactobacillus polysaccharide.

Technical Field

The invention relates to a method for increasing the synthetic amount of lactobacillus polysaccharide, in particular to a culture medium and a method for promoting the synthetic amount of lactobacillus polysaccharide to increase.

Background

Lactic Acid Bacteria (LAB) is a probiotic bacterium of human body, and has effects of promoting digestion, maintaining intestinal health, promoting calcium absorption, preventing diarrhea, inhibiting carcinogen generation, relieving constipation, and making vitamins. The mechanism of the function of lactic acid bacteria, in addition to the effects of living bacteria in colonizing the intestinal tract and regulating the balance of intestinal flora, the substances produced during the growth process, such as bacteriocin, polysaccharide, superoxide dismutase, etc., also play an important role.

Microbial polysaccharide as natural active biological macromolecule is increasingly receiving wide attention from people in the fields of biological medicine, daily chemical industry and the like. The microbial polysaccharide has no toxic or side effect, and has the vital activities of controlling cell differentiation, metabolism, embryonic development, cell cancer, immunoregulation and the like, thereby being widely concerned. The microbial polysaccharide also has the advantages of short production period, no limitation of seasons, regions and pest conditions, low cost, realization of industrial production through fermentation and the like, and people are improving the yield of the commercial polysaccharide through different approaches.

The improvement of microbial productivity has become a hot point of research, and many scientists are continuously making efforts to screen out strains with high microbial polysaccharide yield by means of microbial breeding, but the mutation direction induced by microbial mutation breeding is difficult to grasp, and mutants are difficult to concentrate on a plurality of ideal traits.

Disclosure of Invention

The invention aims to provide a culture medium and a method for promoting the increase of the synthesis amount of lactobacillus polysaccharide, which solve the problem that a strain with high polysaccharide yield is difficult to obtain through microbial breeding, and promote the synthesis of lactobacillus polysaccharide by changing the components of the culture medium.

In order to achieve the above object, the present invention provides a method for increasing the amount of synthesis of a lactic acid bacterial polysaccharide, comprising: the adopted culture medium comprises the following components in parts by weight: 65-75 parts of milk, 4-7 parts of white granulated sugar and 15-19 parts of a gastrodia elata extract; and inoculating lactobacillus strains on the culture medium for fermentation to promote the increase of the synthesis amount of lactobacillus polysaccharide.

Preferably, the lactic acid bacterial species comprises: streptococcus thermophilus and/or Lactobacillus bulgaricus. The strain is derived from Lysima naked yoghourt, and the strain in the yoghourt is two strains of streptococcus thermophilus and lactobacillus bulgaricus.

Preferably, the weight ratio of the gastrodia elata extract is 1: 10 is obtained by extracting rhizoma Gastrodiae powder with water. The said proportion can extract the active components from gastrodia tuber effectively, and the supernatant is added into culture medium to make the active components in certain proportion inside the culture medium.

Preferably, the gastrodia elata powder and water are sheared and stirred at the rotation speed of 10000rpm, and the supernatant obtained by centrifuging the mixed solution is the gastrodia elata extracting solution.

preferably, the centrifugal rotation speed is 8800r/min to prevent insoluble part from precipitating due to too high rotation speed, and prevent solid-liquid separation from being poor due to too low rotation speed.

Preferably, the gastrodia elata powder is obtained by crushing gastrodia elata tubers and then sieving the crushed gastrodia elata tubers with a 180-mesh sieve, and the sieved powder is smaller in particle size, so that the effective substances in the gastrodia elata can be conveniently extracted.

Preferably, the fermentation is carried out at 38-43 ℃.

The invention also provides a culture medium for promoting the increase of the synthesis amount of the lactobacillus polysaccharide, which comprises the following components in parts by weight: 65-75 parts of milk, 4-7 parts of white granulated sugar and 15-19 parts of a gastrodia elata extract.

Preferably, the weight ratio of the gastrodia elata extract is 1: 10 is obtained by extracting rhizoma Gastrodiae powder with water.

The invention also provides a new application of the gastrodia elata, and the gastrodia elata is used for promoting the increase of the synthesis amount of the lactic acid bacteria polysaccharide.

the culture medium and the method for promoting the increase of the synthesis amount of the lactobacillus polysaccharide solve the problem that a strain with high polysaccharide yield is difficult to obtain through microbial breeding, and have the following advantages:

(1) according to the method for increasing the synthesis amount of the polysaccharide, the culture medium containing the gastrodia elata is adopted, so that the yield of the polysaccharide in the growth and metabolism of lactic acid bacteria can be increased;

(2) according to the method for increasing the synthetic amount of the polysaccharide, the polysaccharide is increased by 1241.8mg/L after fermentation in a culture medium added with the gastrodia elata extracting solution compared with a culture medium without the gastrodia elata extracting solution;

(3) According to the method for increasing the synthetic amount of the polysaccharide, the polysaccharide is produced by adopting the lactic acid bacteria, the growth cycle is short, the metabolism is vigorous, the method is not limited by seasons, regions and disease and pest conditions, the culture medium is prepared to be completely solidified, the culture medium added with the gastrodia elata extracting solution only needs 3 hours, the culture medium not added with the gastrodia elata extracting solution needs 4 hours, the production cycle of the lactic acid bacteria polysaccharide is short, and the production cycle can be shortened by adding the gastrodia elata extracting solution;

(4) According to the method for increasing the synthesis amount of the polysaccharide, the materials of the culture medium are all food materials in life, the cost is low, the culture medium is safe and easy to obtain, and the polysaccharide is easy to separate due to the high water content in the fermentation liquor.

drawings

FIG. 1 is a standard graph of glucose.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.