阅读说明：本技术 一种双组氨酸抗菌剂及制备方法和应用 (Double-histidine antibacterial agent, preparation method and application ) 是由 崔海英 张承慧 林琳 朱玉林 于 2019-08-30 设计创作，主要内容包括：本发明属于食品安全领域,具体涉及一种冷等离子体改性的双组氨酸抗菌剂的制备方法和应用。本发明将固体双组氨酸暴露于氮气等离子体中利用冷等离子体对双组氨酸表面进行轰击,得到冷等离子体改性的双组氨酸。与未改性的双组氨酸相比,冷等离子体改性双组氨酸抗菌活性显著提升。其在体外抗菌和抗生物膜中表现出活性提升,且对于生菜表面的生物膜清除效果显著。拓展了其在食品领域的应用。(The invention belongs to the field of food safety, and particularly relates to a preparation method and application of a cold plasma modified bi-histidine antibacterial agent. According to the invention, solid double-histidine is exposed to nitrogen plasma, and the cold plasma is utilized to bombard the surface of the double-histidine, so as to obtain the cold plasma modified double-histidine. Compared with unmodified bishistidine, the cold plasma modified bishistidine has obviously improved antibacterial activity. It has raised activity in vitro antibiotic and biomembrane resisting effect and obvious biomembrane eliminating effect on lettuce surface. The application of the food additive in the food field is expanded.)

1. The double-histidine antibacterial agent is characterized by being prepared by the following steps: the method comprises the steps of bombarding solid double-component amino acid by using cold plasma, improving the bioactivity of the double-component amino acid, preparing a modified double-component amino acid solution, and obtaining a plasma modified double-component amino acid antibacterial agent for dealing with microbial pollution.

2. The bi-histidine antimicrobial agent of claim 1, wherein: the cold plasma takes nitrogen as exciting gas, the flow of the nitrogen is 100sccm, the treatment time of the cold plasma is 3-5 min, and the treatment power is 300-500W.

3. The bi-histidine antimicrobial agent according to claim 1, wherein the modified bi-histidine solution is prepared by a method comprising: dissolving the modified bi-histidine in ether to fully dissolve the modified bi-histidine, and then adding sterile distilled water to prepare the required modified bi-histidine solution.

4. A bi-histidine antimicrobial agent as claimed in claim 3, wherein: the modified bi-histidine is dissolved in ether and then placed on a magnetic stirrer to stir at the room temperature for 1min at the rotation speed of 200 rpm.

5. A bi-histidine antimicrobial agent as claimed in claim 3, wherein: the concentration of the modified double-histidine solution is 5-10mg/mL after sterile distilled water is added.

Technical Field

The invention belongs to the field of food safety, and particularly relates to a cold plasma modified bi-histidine antibacterial agent, and a preparation method and application thereof.

Background

With the improvement of living standard and the progress of technology, people pay more and more attention to food sanitation and safety, which promotes the development of antibacterial agents. The antibacterial agent which is safe, nontoxic and high in antibacterial efficiency is favored by people.

The bi-histidine (His-His, HH) belongs to a novel biological self-assembly material and is an important element for preparing biological functional nano materials. It is formed by dehydration condensation of two histidines, the structure comprises two benzene rings, one end is-NH₂One end of the derivative is-COOH which exists in a zwitterionic form at the isoelectric point, so that the derivative has great potential utilization value.

Plasma (plasma) is a fourth species existing form other than solid, liquid and gas, and is a conductive fluid composed of electrons, ions, radicals and neutral particles, and maintains electric neutrality as a whole. The plasma can be obtained by destroying the molecular structure into atoms by continuously heating the gas, applying a strong electromagnetic field and the like, and ionizing. The gas temperature of the low-temperature plasma is relatively low, and the generated plasma has high density, uniform distribution, convenient utilization and good controllability. The low-temperature plasma is generated after various physical and chemical reactions occur in the generation process: electromagnetic fields, heat, ultraviolet light, charged particles, excited particles, metastable particles, and the like. These active components contain a high enough energy potential to break chemical bonds, which can initiate a series of physicochemical reactions to modify the properties of the substance. CN109569684A discloses a plasma modified metal oxide and g-carbon nitride co-modified titanium dioxide nanorod composite photocatalyst, and a preparation method and an application thereof. CN108409887A discloses a preparation method of antibacterial polysaccharide, which is to carry out atmospheric pressure plasma jet discharge treatment on polysaccharide solution. The above patents all utilize plasma to improve the biological activity of the substance.

The invention utilizes the cold plasma technology to modify the properties of the bi-histidine, and aims to improve the biological activity of the bi-histidine, improve the antibacterial ability of the bi-histidine and expand the application of the bi-histidine in the food industry.

Disclosure of Invention

The invention aims to provide a cold plasma modified bi-histidine antibacterial agent, a preparation method and application thereof in the field of food safety.

The preparation method of the invention comprises the steps of firstly treating solid bi-histidine with cold plasma, and then preparing a modified bi-histidine solution to obtain the modified bi-histidine antibacterial agent.

The cold plasma treatment is performed by using nitrogen as an exciting gas, the flow rate of the nitrogen is 100sccm, the treatment time of the cold plasma is 3-5 min, and the treatment power is 300-500W.

The preparation method of the modified bi-histidine solution comprises the following steps: dissolving the modified bi-histidine into ether to fully dissolve the modified bi-histidine, and then adding distilled water.

The method for dissolving and modifying the bi-histidine by using the diethyl ether is to stir the bi-histidine on a magnetic stirrer at the room temperature of 200rpm for 1 min.

The concentration of the modified double-histidine solution is 5-10mg/mL after distilled water is added.

The preparation cost of the bi-histidine is high, so that the property of the bi-histidine is modified by utilizing charged particles, excited particles and the like generated in the generation process of the low-temperature plasma, so that the biological activity and the antibacterial activity of the bi-histidine are improved. The modified bi-histidine obtained by the invention saves cost and can obtain better antibacterial activity.

Drawings

Figure 1 antimicrobial effect of cold plasma modified two-histidine antimicrobial on free staphylococcus aureus (s.

Figure 2 antibacterial effect of cold plasma modified two-histidine antimicrobial on s.

Figure 3 clearance of cold plasma modified bis-histidine antimicrobial on s.

Figure 4 scavenging effect of cold plasma modified two-component amino acid antibacterial agent on s.

Detailed Description

The following examples illustrate specific embodiments of the present invention, but the scope of the present invention is not limited thereto.