阅读说明：本技术 亚麻荠CsPDAT基因及其在植物抗旱方面的应用 (Camelina sativa CsPDAT gene and application thereof in drought resistance of plants ) 是由 苑丽霞 李润植 于 2019-10-28 设计创作，主要内容包括：本发明植物抗旱基因技术领域,具体涉及一种亚麻荠CsPDAT基因及其在植物抗旱方面的应用。所述亚麻荠基因CsPDAT为CsPDAT2-A,CsPDAT2-A的核苷酸序列如SEQ ID NO.1所示,氨基酸序列如SEQ ID NO.2所示。CsPDAT基因的功能分析和鉴定为获得植物新品种提供了理论基础,利用该基因进行分子育种有望培养出具有生产应用价值的高油植物；为培育抗旱的转基因作物新品种奠定了理论和生产实践基础。(The invention belongs to the technical field of plant drought-resistant genes, and particularly relates to a camelina sativa CsPDAT gene and application thereof in plant drought resistance. The camelina sativa gene CsPDAT is CsPDAT2-A, the nucleotide sequence of CsPDAT2-A is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO. 2. The function analysis and identification of the CsPDAT gene provide a theoretical basis for obtaining new plant varieties, and the gene is expected to be used for molecular breeding to culture high-oil plants with production and application values; lays a theoretical and production practical foundation for cultivating new varieties of drought-resistant transgenic crops.)

1. The camelina sativa gene CsPDAT is characterized in that the camelina sativa gene CsPDAT is CsPDAT2-A, the nucleotide sequence of CsPDAT2-A is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO. 2; the camelina sativa gene CsPDAT can identify plant drought resistance.

2. The camelina sativa gene CsPDAT according to claim 1 for application in drought resistance breeding of plants.

3. The camelina sativa gene CsPDAT according to claim 1 for use in breeding of plants with high oil content.

4. The application of camelina sativa gene CsPDAT in plant breeding with high oil content according to claim 3, wherein the camelina sativa gene CsPDAT further comprises CsPDAT1-A, CsPDAT1-B, CsPDAT1-C and CsPDAT 2-B.

5. Use according to claim 3 or 4, wherein the plants are camelina sativa and tobacco.

Technical Field

The invention belongs to the technical field of plant drought-resistant genes, and particularly relates to a camelina sativa CsPDAT gene and application thereof in plant drought resistance.

Background

Camelina sativa (Camelina sativa l.cranz) is a cruciferous oil crop widely planted in north america and european regions, and the planting area is increasingly expanded due to its excellent biological and agronomic characteristics, such as low consumption, high resistance and environmental protection. The camelina sativa has short growth period (80-100d), average plant height of 92.4cm, average thousand seed mass of 1.25g and high oil content (34-45%) in seeds, and introduction, breeding and popularization planting of camelina sativa are also developed in recent years in China. The unique unsaturated fatty acid composition of the camelina sativa seeds, as well as the high level of omega-3 fatty acids and the low content of glucosinolates, endow the camelina sativa oil with important food nutritional values and industrial utilization values. Furthermore, camelina sativa seeds also contain storage proteins, which represent about 30% of the dry mass of the seeds, and are an important source of human food and animal feed proteins. The camelina sativa is an oil crop with low investment and stress tolerance, and has lower requirements on water and fertilizer. Compared with wheat and rape, the water demand and fertilizer consumption of camelina sativa are obviously reduced, and the production investment is only about half of that of rape. Camelina sativa can also grow normally under relatively barren soil conditions (e.g., non-rural marginal lands such as gullies, roadside, wilderness, saline-alkali land, etc.). The camelina sativa is popularized and planted in arid northern areas of China, so that the shortage of agricultural water resources in northern areas can be relieved, the ecological environment is protected, and the production value is high.

Drought and water resource shortage become major limiting factors of world agricultural development, and pose serious threats to agricultural production. The camelina sativa has excellent characteristics of drought tolerance, barren tolerance, disease resistance, insect resistance and the like, is an important germplasm resource for genetic improvement of cruciferous crops, better meets the development requirement of sustainable agriculture, and is a relatively ideal novel energy crop. In the aspect of breeding camelina sativa drought-resistant varieties, the most common method is conventional cross breeding, wherein the conventional cross breeding is a method for obtaining individuals with recombined parental genes through mating among individuals with different genotypes. New genes can not be generated in the hybridization process, and the hybrid progeny can have character separation, the breeding process is slow, and the process is complex. Therefore, the development of the gene capable of identifying the camelina sativa drought resistance has important significance for breeding camelina sativa drought-resistant varieties.

Disclosure of Invention

The invention provides a camelina sativa CsPDAT gene and application thereof in plant drought resistance, wherein the CsPDAT2-A gene can improve the drought resistance of camelina sativa.

The camelina sativa gene CsPDAT is CsPDAT2-A, the nucleotide sequence of CsPDAT2-A is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO. 2.

The invention also provides application of the camelina sativa gene CsPDAT in plant drought resistance breeding.

The invention also provides application of the camelina sativa gene CsPDAT in plant breeding with high oil content.

Preferably, according to the application, the camelina sativa gene CsPDAT further comprises CsPDAT1-A, CsPDAT1-B, CsPDAT1-C and CsPDAT 2-B.

Preferably, according to the above application, the plants are camelina sativa and tobacco.

Compared with the prior art, the camelina sativa CsPDAT gene and the application thereof in the aspect of plant drought resistance have at least the following beneficial effects:

the function analysis and identification of the CsPDAT gene provide a theoretical basis for obtaining new plant varieties, and the gene is expected to be used for molecular breeding to culture high-oil plants with production and application values. Lays a theoretical and production practical foundation for cultivating new varieties of drought-resistant transgenic crops.

The results of our study show that drought stress treatment causes 2-fold increase of CsPDAT2-A mRNA expression, and cDNA clones of the CsPDAT genes are also isolated and used for transient expression of tobacco leaves, and analysis shows that the activity of the five genes (CsPDAT1-A, CsPDAT1-B, CsPDAT1-C, CsPDAT2-A and CsPDAT2-B) PDAT enzyme and lipid accumulation are greatly increased.

Drawings

FIG. 1 is the accumulation of TAG in camelina sativa seedlings over time under drought stress;

FIG. 2 shows that CsPDAT members exhibit three distinct patterns of seedling mRNA expression under drought stress;

FIG. 3 shows the TAG content of CsPDAT transiently expressed in tobacco leaves.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.

The following examples and test methods not specifically described in the above summary of the invention were carried out according to conventional methods and conditions in the art, and the materials used were commercially available unless otherwise specified.

The invention provides a camelina sativa gene CsPDAT capable of identifying plant drought resistance, wherein the camelina sativa gene CsPDAT is CsPDAT2-A, the nucleotide sequence of CsPDAT2-A is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO. 2. CsPDAT2-A is located on chromosome IV in cells, and has 6 exons, and encodes a 662 amino acid protein.

The CsPDAT can code phosphatidylcholine diacylglycerol acyltransferase, the enzyme can introduce acyl in Phosphatidylcholine (PC) molecules into DAG to finally generate TAG (triacylglycerol), the enzyme is an important enzyme participating in the last acylation reaction for generating TAG, and the higher the expression level of the enzyme is, the stronger the grease-producing performance of camelina sativa is.

The invention also provides application of the CsPDAT2-A gene shown as SEQ ID NO.1 in plant drought resistance breeding, in particular to camelina sativa drought resistance breeding.