阅读说明：本技术 用于脱除霉菌毒素的脱毒剂、制备方法及应用 (For removing detoxifying agent, the preparation method and application of mycotoxin ) 是由 申伟贤 于 2019-08-22 设计创作，主要内容包括：本发明涉及一种用于脱除霉菌毒素的脱毒剂、制备方法及应用,该用于脱除霉菌毒素的脱毒剂由以下重量份的各原料制成：水合硅铝钙钠盐：1~2份,枯草芽孢杆菌：0.5~0.7份,酵母粉：0.5~0.7份,酵母细胞壁：28~30份,蒙脱石：63~66份,植酸酶：3~5份,苯甲酸：0.1~0.3份,其中酵母细胞壁中至少包含甘露寡糖以及?-葡聚糖。本发明提出的用于脱除霉菌毒素的脱毒剂,可有效脱除饲料中的霉菌毒素,提高了配方饲料的安全性以及饲料功效,满足了实际饲养需求。(The detoxifying agent that the present invention relates to a kind of for removing mycotoxin, preparation method and application, the detoxifying agent for being used to remove mycotoxin is made of each raw material of following parts by weight: hydration sial calcium disodium: 1 ~ 2 part, bacillus subtilis: 0.5 ~ 0.7 part, yeast powder: 0.5 ~ 0.7 part, yeast cell wall: 28 ~ 30 parts, montmorillonite: 63 ~ 66 parts, phytase: 3 ~ 5 parts, benzoic acid: 0.1 ~ 0.3 part, manna oligosacchride and-glucan are wherein included at least in yeast cell wall.The detoxifying agent proposed by the present invention for being used to remove mycotoxin, can effectively remove the mycotoxin in feed, improve the safety and feed effect of formula forage, meet practical raising demand.)

1. a kind of for removing the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent is prepared from the following raw materials:

It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid；

Raw material needed for wherein preparing the detoxifying agent of Unit Weight includes following each components by weight percent:

Hydration sial calcium disodium: 1 ~ 2 part；Bacillus subtilis: 0.5 ~ 0.7 part；

Yeast powder: 0.5 ~ 0.7 part；Yeast cell wall: 28 ~ 30 parts:

Montmorillonite: 63 ~ 66 parts；Phytase: 3 ~ 5 parts；

Benzoic acid: 0.1 ~ 0.3 part；

Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.

2. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight Stating the corresponding each composition by weight of detoxifying agent includes following component:

Hydration sial calcium disodium: 1 part；Bacillus subtilis: 0.5 part；

Yeast powder: 0.5 part；Yeast cell wall: 28 parts:

Montmorillonite: 63 parts；Phytase: 5 parts；

Benzoic acid: 0.1 part；

Wherein, the bacillus subtilis is YB4.

3. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight Stating the corresponding each composition by weight of detoxifying agent includes following component:

Hydration sial calcium disodium: 2 parts；Bacillus subtilis: 0.7 part；

Yeast powder: 0.7 part；Yeast cell wall: 30 parts:

Montmorillonite: 66 parts；Phytase: 5 parts；

Benzoic acid: 0.1 part；

Wherein, the bacillus subtilis is YB4.

4. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight Stating the corresponding each composition by weight of detoxifying agent includes following component:

Hydration sial calcium disodium: 1.5 parts；Bacillus subtilis: 0.6 part；

Yeast powder: 0.6 part；Yeast cell wall: 29.6 parts:

Montmorillonite: 65 parts；Phytase: 4 parts；

Benzoic acid: 0.2 part；

Wherein, the bacillus subtilis is YB4.

5. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent further includes Bacillus coagulans and cellulase, the corresponding parts by weight of the bacillus coagulans are 0.5 ~ 1 part, the cellulase Corresponding weight fraction is 1.2 ~ 1.5 parts.

6. according to claim 5 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight Stating the corresponding each composition by weight of detoxifying agent includes following component:

Hydration sial calcium disodium: 1.5 parts；Bacillus subtilis: 0.6 part；

Yeast powder: 0.6 part；Yeast cell wall: 29.6 parts:

Montmorillonite: 65 parts；Phytase: 4 parts；

Benzoic acid: 0.2 part；Bacillus coagulans: 0.5 part；

Cellulase: 1.3 parts；

Wherein, the bacillus subtilis is YB4.

7. a kind of for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent is such as above-mentioned power Benefit requires detoxifying agent described in 1 to 6 any one, and the preparation method includes the following steps:

Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de- Toxic agent raw material slurry；

Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, is added and steams Distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor；

Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 25 DEG C ~ 30 DEG C At a temperature of stand 3 ~ 5h, then in the environment of 35 DEG C ~ 45 DEG C stand until moisture be evaporated to obtain the detoxifying agent；

Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.

8. according to claim 7 for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that preparation is single Raw material needed for the detoxifying agent of position weight includes following each components by weight percent:

Hydration sial calcium disodium: 1 ~ 2 part；Bacillus subtilis: 0.5 ~ 0.7 part；

Yeast powder: 0.5 ~ 0.7 part；Yeast cell wall: 28 ~ 30 parts:

Montmorillonite: 63 ~ 66 parts；Phytase: 3 ~ 5 parts；

Benzoic acid: 0.1 ~ 0.3 part.

9. according to claim 7 for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that preparing After the step of obtaining the active bacteria mixed liquor, the method also includes:

Bacillus coagulans and cellulase are continuously added into the active bacteria mixed liquor, it is quiet after at the uniform velocity 10 ~ 15min of stirring 2 ~ 3h is set, and the mixed liquor obtained after standing is poured into the detoxifying agent raw material slurry and is stirred.

10. a kind of for removing the application of the detoxifying agent of mycotoxin, which is characterized in that application such as the claims 1 to 6 The mycotoxin in feed is removed for removing the detoxifying agent of mycotoxin described in meaning one, the mycotoxin packet Include at least one of aflatoxin, zearalenone, vomitoxin and fumarubicin.

Technical field

The present invention relates to field of feed additive technology, in particular to a kind of detoxifying agent for removing mycotoxin, system Preparation Method and application.

Background technique

It is well known that feed is essential important source material in breeding technology field.For feed, all kinds of feeds In raw material and all kinds of compound feed products, inevitably can all there be mycotoxin contamination, recall rate is even up to 70%~100%.

For food pollution, 7~8 kinds of Duo Zeyou, a variety of moulds are presented in few then 2~3 kinds of detection toxin for polluting feed The feature of toxin combined pollution.In view of the particularity of mycotoxin, to fungus growth breeding regulation and mould condition, mycotoxin are produced Metabolic residue rule, toxicity of mycotoxins and its machine of distribution and development law, mycotoxin in animal body in feed Fast and accurately detection device and technology detecting method etc. are required to for system, the reciprocal effects of a variety of mycotoxins and mycotoxin It carries out going deep into system research.Since mycotoxin has biggish harmfulness, it is therefore desirable to be removed to it.Currently, mould Detoxicated dose of product mainly includes 2 major class of mold toxin sorbent and biological enzymolysis toxic agent, and mold toxin sorbent according to The difference of its component can be divided into active carbon class, mannans, Aluminosilicates and biological species etc..Toxin is added in feed to inhale Attached dose is the mycotoxin eliminating feed method being most widely used in current Production of Livestock and Poultry.

However, existing toxin agent has also adsorbed minerals, the vitamin in feed while absorbing mycotoxin Etc. nutritional ingredients, influence the absorption of nutriment.

Summary of the invention

Based on this, the purpose of the present invention is to solve existing toxin agent also to adsorb while absorbing mycotoxin Minerals, vitamins and other nutritious components in feed, the problem of affecting the absorption of nutriment.

The present invention proposes a kind of for removing the detoxifying agent of mycotoxin, wherein the detoxifying agent is prepared from the following raw materials:

It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid；

Raw material needed for wherein preparing the detoxifying agent of Unit Weight includes following each components by weight percent:

Hydration sial calcium disodium: 1 ~ 2 part；Bacillus subtilis: 0.5 ~ 0.7 part；

Yeast powder: 0.5 ~ 0.7 part；Yeast cell wall: 28 ~ 30 parts:

Montmorillonite: 63 ~ 66 parts；Phytase: 3 ~ 5 parts；

Benzoic acid: 0.1 ~ 0.3 part；

Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.

The detoxifying agent proposed by the present invention for being used to remove mycotoxin, by hydration sial calcium disodium, bacillus subtilis, ferment The raw materials such as female powder, yeast cell wall, montmorillonite, phytase and benzoic acid are made, wherein hydration sial calcium disodium, it can be to including Mycotoxin adsorption capacity with super strength including aflatoxin；In yeast cell wall-glucan ingredient, have efficient The mycotoxin (such as zearalenone) for adsorbing low pole, using bacillus subtilis conversion technology by Gibberella zeae Ketenes is converted into nontoxic metabolin, strengthens detoxicating functions；Manna oligosacchride in yeast cell wall can be colonized to be tied on stomach surface Protective film is formed on coincidence point, prevents the infringement of harmful bacteria, and is excreted in conjunction with the harmful bacterias such as Escherichia coli in enteron aisle, Manna oligosacchride and bacillus subtilis promote the production and breeding of beneficial bacterium in animal body simultaneously, to inhibit the life of harmful bacteria It is long, improve function of intestinal canal and health；Oligosaccharides and marine algae extract synergistic effect in yeast cell wall, intensify immunity of organism machine Meeting enhances Immune Function In Animals.To sum up, above-mentioned detoxifying agent targetedly can effectively remove the toxin in feed, Without the absorption of the nutriments such as influence minerals therein, vitamin.

The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy Measuring component includes following component:

Hydration sial calcium disodium: 1 part；Bacillus subtilis: 0.5 part；

Yeast powder: 0.5 part；Yeast cell wall: 28 parts:

Montmorillonite: 63 parts；Phytase: 5 parts；

Benzoic acid: 0.1 part；

Wherein, the bacillus subtilis is YB4.

The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy Measuring component includes following component:

Hydration sial calcium disodium: 2 parts；Bacillus subtilis: 0.7 part；

Yeast powder: 0.7 part；Yeast cell wall: 30 parts:

Montmorillonite: 66 parts；Phytase: 5 parts；

Benzoic acid: 0.1 part；

Wherein, the bacillus subtilis is YB4.

The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy Measuring component includes following component:

Hydration sial calcium disodium: 1.5 parts；Bacillus subtilis: 0.6 part；

Yeast powder: 0.6 part；Yeast cell wall: 29.6 parts:

Montmorillonite: 65 parts；Phytase: 4 parts；

Benzoic acid: 0.2 part；

Wherein, the bacillus subtilis is YB4.

The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent further includes bacillus coagulans and fibre Tie up plain enzyme, the corresponding parts by weight of the bacillus coagulans are 0.5 ~ 1 part, and the corresponding weight fraction of the cellulase is 1.2 ~ 1.5 parts.

The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy Measuring component includes following component:

Hydration sial calcium disodium: 1.5 parts；Bacillus subtilis: 0.6 part；

Yeast powder: 0.6 part；Yeast cell wall: 29.6 parts:

Montmorillonite: 65 parts；Phytase: 4 parts；

Benzoic acid: 0.2 part；Bacillus coagulans: 0.5 part；

Cellulase: 1.3 parts；

Wherein, the bacillus subtilis is YB4.

The present invention also proposes a kind of for removing the preparation method of the detoxifying agent of mycotoxin, wherein the detoxifying agent is Detoxifying agent as described above, the preparation method include the following steps:

Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de- Toxic agent raw material slurry；

Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, is added and steams Distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor；

Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 25 DEG C ~ 30 DEG C At a temperature of stand 3 ~ 5h, then in the environment of 35 DEG C ~ 45 DEG C stand until moisture be evaporated to obtain the detoxifying agent；

Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.

The preparation method of the detoxifying agent for being used to remove mycotoxin, wherein prepare the detoxifying agent of Unit Weight Required raw material includes following each components by weight percent:

Hydration sial calcium disodium: 1 ~ 2 part；Bacillus subtilis: 0.5 ~ 0.7 part；

Yeast powder: 0.5 ~ 0.7 part；Yeast cell wall: 28 ~ 30 parts:

Montmorillonite: 63 ~ 66 parts；Phytase: 3 ~ 5 parts；

Benzoic acid: 0.1 ~ 0.3 part.

The preparation method of the detoxifying agent for being used to remove mycotoxin, wherein the active bacteria mixing is being prepared After the step of liquid, the method also includes:

Bacillus coagulans and cellulase are continuously added into the active bacteria mixed liquor, it is quiet after at the uniform velocity 10 ~ 15min of stirring 2 ~ 3h is set, and the mixed liquor obtained after standing is poured into the detoxifying agent raw material slurry and is stirred.

The present invention also proposes a kind of for removing the application of the detoxifying agent of mycotoxin, wherein application is as described above to be used In removing mycotoxin detoxifying agent the mycotoxin in feed is removed, the mycotoxin include aflatoxin, At least one of zearalenone, vomitoxin and fumarubicin.

Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description Obviously, or practice through the invention is recognized.

Specific embodiment

To facilitate the understanding of the present invention, below with reference to related embodiment to invention is more fully described.Implement Preferred embodiment of the invention is given in example.But the invention can be realized in many different forms, however it is not limited to this Literary described embodiment.On the contrary, purpose of providing these embodiments is make it is more thorough to the disclosure complete Face.

Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term " and or " used herein includes one or more phases Any and all combinations of the listed item of pass.

Existing toxin agent has also adsorbed the nutrition such as minerals, the vitamin in feed while absorbing mycotoxin Ingredient influences the absorption of nutriment.

In order to solve the above-mentioned technical problem, the present invention proposes a kind of for removing the detoxifying agent of mycotoxin, wherein this is de- Toxic agent is prepared from the following raw materials:

It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid.

Specifically, raw material needed for preparing the detoxifying agent of Unit Weight includes following each heavy in actual fabrication process Measure component:

Hydration sial calcium disodium: 1 ~ 2 part；Bacillus subtilis: 0.5 ~ 0.7 part；

Yeast powder: 0.5 ~ 0.7 part；Yeast cell wall: 28 ~ 30 parts:

Montmorillonite: 63 ~ 66 parts；Phytase: 3 ~ 5 parts；

Benzoic acid: 0.1 ~ 0.3 part.

It should be added that, for above-mentioned yeast cell wall, including at least in yeast cell wall has herein Manna oligosacchride and-glucan.

The detoxifying agent proposed by the present invention for being used to remove mycotoxin, by hydration sial calcium disodium, bacillus subtilis, ferment The raw materials such as female powder, yeast cell wall, montmorillonite, phytase and benzoic acid are made, wherein hydration sial calcium disodium, it can be to including Mycotoxin adsorption capacity with super strength including aflatoxin；In yeast cell wall-glucan ingredient, have efficient The mycotoxin (such as zearalenone) for adsorbing low pole, using bacillus subtilis conversion technology by Gibberella zeae Ketenes is converted into nontoxic metabolin, strengthens detoxicating functions；Manna oligosacchride in yeast cell wall can be colonized to be tied on stomach surface Protective film is formed on coincidence point, prevents the infringement of harmful bacteria, and is excreted in conjunction with the harmful bacterias such as Escherichia coli in enteron aisle, Manna oligosacchride and bacillus subtilis promote the production and breeding of beneficial bacterium in animal body simultaneously, to inhibit the life of harmful bacteria It is long, improve function of intestinal canal and health；Oligosaccharides and marine algae extract synergistic effect in yeast cell wall, intensify immunity of organism machine Meeting enhances Immune Function In Animals.To sum up, above-mentioned detoxifying agent targetedly can effectively remove the toxin in feed, Without the absorption of the nutriments such as influence minerals therein, vitamin.

At the same time, the present invention also proposes a kind of for removing the preparation method of the detoxifying agent of mycotoxin, wherein described Detoxifying agent is detoxifying agent as described above, and the preparation method includes the following steps:

Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de- Toxic agent raw material slurry.

Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added Enter distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor.

Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly 25 DEG C ~ 30 3 ~ 5h is stood at a temperature of DEG C, is then stood in the environment of 35 DEG C ~ 45 DEG C until moisture is evaporated to obtain the detoxifying agent.

It it should be added that, include herein sweet dew in yeast cell wall for above-mentioned yeast cell wall Oligosaccharides and-glucan.

Specific embodiments of the present invention are discussed in further detail with several specific embodiments below: