阅读说明：本技术 一种快速定性比较海藻原料中岩藻多糖含量的方法 (A kind of fast qualitative compares the method for fucoidin content in seaweed raw material ) 是由 申培丽 张茂超 王盼 尹宗美 李可昌 于 2019-08-27 设计创作，主要内容包括：本发明公开了一种快速定性比较海藻原料中岩藻多糖含量的方法,包括以下步骤：称取适量磨碎的海藻样品,烘干；称取干海藻样品置于烧杯中,并加入纯水,搅拌均匀制得海藻溶液；将上述海藻溶液置于水浴中恒温加热后再冷却至室温；进行过滤,并收集滤液；用移液管吸取滤液；使移液管自然竖直,用秒表计时滤液从自然流出至液位降至标记处所需的时间,记录时间数据；重复多次,取平均时间值。本发明的有益效果是,利用相同体积的溶液通过同一孔径的细孔时,所需时间与溶液粘度正相关的原理,通过水浴提取法,对提取液的粘度进行快速定性比较；通过移液管和秒表比较液体粘度大小,从而实现定性比较海藻原料中岩藻多糖含量的目的。(The invention discloses a kind of methods that fast qualitative compares fucoidin content in seaweed raw material, comprising the following steps: weighs the seaweed sample ground in right amount, dries；It weighs dry seaweed sample to be placed in a beaker, and pure water is added, stir evenly obtained seaweed solution；Above-mentioned seaweed solution is placed in water-bath and is cooled to room temperature after heated at constant temperature；It is filtered, and collects filtrate；Filtrate is drawn with pipette；Keep pipette naturally vertical, with manual time-keeping filtrate from the time needed for liquid level is down to mark is flowed out to naturally, records time data；Repeatedly, average time value is taken.The invention has the advantages that required time and the positively related principle of solution viscosity carry out fast qualitative comparison to the viscosity of extracting solution by water-bath extraction method when the solution using same volume passes through the pore in same aperture；By pipette and stopwatch comparative liquid viscosity size, to realize the purpose of fucoidin content in qualitative relatively seaweed raw material.)

1. a kind of fast qualitative compares the method for fucoidin content in seaweed raw material, which comprises the following steps:

A, the seaweed sample ground in right amount is weighed, is dried 4 hours；

B, 50 grams of dry seaweed sample are weighed, is placed in a beaker, and 500mL pure water is added, stirs evenly obtained seaweed solution；

C, above-mentioned seaweed solution is placed in 70 DEG C of water-baths, is cooled to room temperature after heated at constant temperature 2h；

D, upper seaweed solution after cooling is filtered, and collects filtrate；

E, the pipette of specification 50mL is selected, and is made marks at pipette distance outlet 3-5cm in advance；

F, the filtrate 50mL being collected into is drawn with above-mentioned pipette；

G, keep pipette naturally vertical, with manual time-keeping filtrate from natural outflow, until liquid level be down to mark needed for the time, note Record time data；

H, it repeats step f, g more times, takes average time value.

2. a kind of fast qualitative as described in claim 1 compares the method for fucoidin content in seaweed raw material, feature exists In in step a, seaweed sample is dried to moisture less than 1.0%, after grinding, sieves with 100 mesh sieve.

3. a kind of fast qualitative as described in claim 1 compares the method for fucoidin content in seaweed raw material, feature exists In in step c, beaker is sealed in heated at constant temperature, temperature-fall period, prevents the moisture evaporation in seaweed solution.

4. a kind of fast qualitative as described in claim 1 compares the method for fucoidin content in seaweed raw material, feature exists In in step d, filtering selects 60 mesh with screen specification.

5. a kind of fast qualitative as described in claim 1 compares the method for fucoidin content in seaweed raw material, feature exists In, in step e, with marking pen in pipette away from being drawn a line at the liquid outlet 3-5cm of bottom end.

6. a kind of fast qualitative as described in claim 1 compares the method for fucoidin content in seaweed raw material, feature exists In the seaweed sample is the brown algae rich in fucoidin, including in kelp, opotism, sargassum fusifome, sargassum and yellow tang It is a kind of.

Technical field

The present invention relates to seaweed process and utilization technology fields more particularly to a kind of fast qualitative to compare rock algae in seaweed raw material The method of polyoses content.

Background technique

Polysaccharide component rich in, such as algin in brown alga, fucoidin etc. are widely used in food and medicine etc. Field.Wherein, fucoidin is iuntercellular polysaccharide intrinsic in brown alga, is present in cell wall matrix.Studies have shown that rock algae Polysaccharide, which has, improves immunity, anticoagulation, antitumor, antiviral, anti-oxidant, promotion skin regeneration, skin moisture-keeping etc. more than 20 Item physiological function.

Fucoidin main component is L-fucose, and linear backbone is usually fucose with α -1, and 3 or α-Isosorbide-5-Nitrae glycosidic bond connects It connects, while with the sulphation heteroglycan of the monosaccharide components such as galactolipin, mannose, glucose, xylose.The content of fucose There is correlative connection with the biological activity of fucoidin.Therefore, the fucoidin content in seaweed is generally to detect fucose Subject to content.

Currently, extracting fucoidin, simplest method is water extraction, because containing the macromolecular complex such as fucoidin in filtrate Matter, and filtrate viscosity is directly proportional to fucoidin content.Therefore, filtrate viscosity, which can be used as, compares one of fucoidin content Index.However, frond type is different, the content of fucoidin is also variant, the seaweed of same breed, because of sea area, harvesting season The difference of section, processing method, the content of fucoidin are also not quite similar.To produce fucoidin, it is often necessary to seaweed original The indexs such as the fucoidin content of material, price are compared.Currently, it is ratio that the method for detection fucoidin content is currently used Color method and chromatography.The operating process of both methods is more complicated, and the requirement to experiment reagent, equipment, personnel is also relatively high, In addition, filtrate viscosity is measured with viscosimeter, it is higher to device requirement, and deviation is larger.

Summary of the invention

To solve above-mentioned fucoidin comparision contents big, the complicated for operation technical problem of existing deviation in the process, this hair The bright a kind of method for disclosing fast qualitative and comparing fucoidin content in seaweed raw material.

To achieve the above object, the present invention adopts the following technical solutions:

A kind of fast qualitative compares the method for fucoidin content in seaweed raw material, comprising the following steps:

A, the seaweed sample ground in right amount is weighed, is dried 4 hours；

B, 50 grams of dry seaweed sample are weighed, is placed in a beaker, and 500mL pure water is added, stirs evenly obtained seaweed solution；

C, above-mentioned seaweed solution is placed in 70 DEG C of water-baths, is cooled to room temperature after heated at constant temperature 2h；

D, upper seaweed solution after cooling is filtered, and collects filtrate；

E, the pipette of specification 50mL is selected, and is made marks at pipette distance outlet 3-5cm in advance；

F, the filtrate 50mL being collected into is drawn with above-mentioned pipette；

G, keep pipette naturally vertical, with manual time-keeping filtrate from natural outflow, until when liquid level is down to needed for mark Between, record time data；

H, it repeats step f, g more times, takes average time value.

Further, in step a, seaweed sample is dried to moisture less than 1.0%, after grinding, sieves with 100 mesh sieve.

Further, in step c, beaker is sealed in heated at constant temperature, temperature-fall period, prevents the moisture in seaweed solution from waving Hair.

Further, in step d, filtering selects 60 mesh with screen specification.

Further, in step e, with marking pen in pipette away from being drawn a line at the liquid outlet 3-5cm of bottom end.

Further, the seaweed sample is the brown algae rich in fucoidin, including kelp, opotism, sargassum fusifome, horse hair One of algae and yellow tang.

The invention has the advantages that using the solution of same volume by the pore in same aperture when, required time with The positively related principle of solution viscosity carries out fast qualitative comparison to the viscosity of extracting solution by water-bath extraction method；In advance in liquid relief A label is done at pipe outlet 3-5cm, prevents surface tension from interfering timing；By pipette and stopwatch comparative liquid viscosity size, To realize the purpose of fucoidin content in qualitative relatively seaweed principle.

Specific embodiment

The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment It is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.