阅读说明：本技术 酶解葛根醒酒复合片及其制备方法 (Enzymolysis radix puerariae composite tablet for dispelling effects of alcohol and preparation method thereof ) 是由 傅荣昭 吴仕清 周文俊 于 2021-08-05 设计创作，主要内容包括：本发明属于生物功能性食品制剂领域,具体涉及一种酶解葛根醒酒复合片及其制备方法,配方由以下组分按重量份组成：山梨糖醇10-20份、枳椇子5-10份、酶解葛根6-10份、玉米低聚肽3-5份、硬脂酸镁0.1-0.2份、甜菊糖苷0.1-0.15份,在新鲜葛根时对其施用多酶组合溶液A液,保持其内的大豆黄酮浓度,降解淀粉等,防止呼吸作用大豆黄酮,在葛根萃取液中添加多酶组合溶液B液进一步浓缩大豆黄酮浓度。本工艺制备的酶解葛根对酒后产生的头疼有良好的抑制作用。(The invention belongs to the field of biological functional food preparations, and particularly relates to an enzymolysis radix puerariae sobering composite sheet and a preparation method thereof, wherein the enzymolysis radix puerariae sobering composite sheet comprises the following components in parts by weight: 10-20 parts of sorbitol, 5-10 parts of hovenia dulcis thunb, 6-10 parts of enzymolysis radix puerariae, 3-5 parts of corn oligopeptide, 0.1-0.2 part of magnesium stearate and 0.1-0.15 part of stevioside, wherein the soybean flavone concentration is kept by applying a multi-enzyme combined solution A to fresh radix puerariae, starch and the like are degraded, the soybean flavone with respiration is prevented, and the soybean flavone concentration is further concentrated by adding a multi-enzyme combined solution B to radix puerariae extract. The enzymolysis radix puerariae prepared by the process has a good inhibition effect on headache generated after drinking.)

1. The enzymolysis radix puerariae composite tablet for sobering up and the preparation method thereof are characterized by comprising the following components: sorbitol, semen Hoveniae, enzymolysis radix Puerariae, semen Maydis oligopeptide, magnesium stearate, and stevioside.

2. The formulation according to claim 1, characterized by consisting of the following components in parts by weight: 10-20 parts of sorbitol, 5-10 parts of semen hoveniae, 6-10 parts of enzymolysis radix puerariae, 3-5 parts of corn oligopeptide, 0.1-0.2 part of magnesium stearate and 0.1-0.15 part of stevioside.

3. The enzymatic hydrolyzed kudzu root according to claim 2, wherein the preparation method is as follows:

(1) uniformly spraying the multienzyme combined solution A on the surface of fresh radix Puerariae picked within 3 days according to the dosage of 100mL/kg, compacting, and acting in an oxygen-free closed environment for 7 days;

(2) squeezing for dewatering, and hot air drying at 80 deg.C for 60 min until the water content is less than or equal to 15% to obtain processed radix Puerariae;

(3) adding dehydrated radix Puerariae into 1kg of ethanol 3-5L, performing static extraction at room temperature for 24 hr, and collecting extractive solution;

(4) concentrating the extract liquid by a vacuum rotary dryer at 60 ℃, 0.03MPa and 30rpm until no distillate exists;

(5) adding deionized water into the radix puerariae extract obtained in the step (4) according to the volume ratio of 1:2, and adding the multienzyme combined solution B into the mixed solution again according to the proportion of 10ml to 1L; reacting at 45 ℃ and 120rpm for 24 hours;

(6) standing for 12 hr, separating water phase and organic phase, removing water phase, collecting organic phase, spray drying, and granulating to obtain zymolytic radix Puerariae.

4. The solution A of the multi-enzyme combined solution according to claim 3, which comprises the following enzymes in parts by weight: 20 parts of amylase, 10 parts of phosphatase, 5 parts of endo beta-glucanase, 18 parts of pectinase, 10 parts of cutinase, 7 parts of protease and 5000 parts of distilled water.

5. The solution B of the multi-enzyme combined solution according to claim 3, which comprises the following enzymes in parts by weight: 10 parts of endoxylanase, 10 parts of cellulase, 6 parts of phosphatase, 5 parts of cutinase, 7 parts of protease and 5000 parts of distilled water.

6. The use of the pueraria sobering composite tablet of claim 2 for sobering up.

Technical Field

The invention belongs to the field of biological functional food preparations, and particularly relates to an enzymolysis radix puerariae sobering composite sheet and a preparation method thereof.

Background

Kudzu root, the name of traditional Chinese medicine, is the dried root of kudzu of leguminous plant, commonly known as kudzu, harvested in autumn and winter, and cut into thick slices or small blocks when the kudzu is fresh; the kudzu root is plant gold, contains 13 amino acids essential to human body, and especially has higher content of essential amino acids which cannot be synthesized by human body. The effective active ingredients contained in the composition comprise isoflavone, puerarin, alkaloid and other substances, and trace elements such as iron, calcium, manganese and the like which are necessary for human bodies are also rich.

Radix Puerariae and its product have effects of invigorating yang, relieving hunger, promoting fluid production, quenching thirst, clearing pathogenic fire, removing toxic substance, reducing blood lipid, lowering blood pressure, lowering blood sugar, reducing cholesterol, and preventing cardiovascular and cerebrovascular diseases. According to the modern medical research, the pueraria isoflavone has the effects of cancer prevention and cancer resistance and plant stimulation, has extremely high drinking value, is called long-life dragon root every day, is called Chinese yam treasure, has the reputation of Asian ginseng internationally, is called pachyrhizua angulatus, is particularly large in pueraria, has the starch content of 25-30 percent, is called pachyrhizua angulatus due to the fact that the pueraria angulatus contains more pueraria starch, is called pachyrhizua angulatus due to the fact that the pueraria angulatus is sweet, is mainly and wild in low mountain valley, is not artificially cultured and domesticated, and has the starch content of 17-20 percent. The kudzu root powder is cool and pungent, has various functions of relieving exterior syndrome, reducing fever, promoting production of body fluid, promoting eruption, invigorating yang and stopping diarrhea and the like, can treat various diseases such as fever and headache caused by external infection, high blood pressure and neck pain, thirst, diabetes, measles without adequate eruption, heat eruption, diarrhea and the like, contains 12% of flavonoid compounds such as puerarin, soybean flavonoid glycoside, peanut essence and other nutritional ingredients, and also contains protein, amino acid, sugar, mineral substances such as iron, calcium, copper, selenium and the like which are necessary for human bodies, is a famous and precious tonic product suitable for people of all ages, has the reputation of ' thousand years ginseng ', has a famous prescription ' in the typhoid treatise on the typhoid fever of Zhongjing in the Han Dynasty, remains an important exterior syndrome till now, and the kudzu root in the spleen and stomach is the best capable of developing the qi of clearing yang, has sweet and fragrant smell, cool nature and is mainly used in the spleen and stomach channels. Has the functions of relieving exterior syndrome, expelling pathogenic factors from muscles, invigorating yang, promoting eruption, relieving fever and promoting the production of body fluid. Can be used for treating spleen deficiency, diarrhea, fever thirst, fever, headache, neck pain, measles without adequate eruption, epidemic febrile disease thirst, diabetes, alcoholism, thoracic obstruction, cardiodynia, etc., and has effects of regulating human body function, enhancing body constitution, improving organism disease resistance, resisting aging, prolonging life, and maintaining youthful vigor.

The kudzu root has a plurality of effects because the kudzu root contains more flavonoids, and researches show that the flavonoids can effectively increase blood flow of brain and coronary vessels, and headache is easy to occur when people wake up the next day after drunkenness because ethanol in alcohol is converted into acetaldehyde after the metabolism of liver. Acetaldehyde has a remarkable effect of dilating capillaries, and particularly has a remarkable effect of dilating capillaries in the head, so that headache is caused. The common method for relieving headache is to take some pain relieving medicines, such as pain relieving tablets, ibuprofen, pain relieving powder and other western medicines, but the medicines have certain side effects, and the pueraria flavonoids compound can effectively increase blood flow of brain and coronary vessels, has good spasmolysis effect, and can resist the transmission of histamine and acetylcholine, thereby relieving headache.

Some techniques for sobering up with pueraria lobata exist in the prior art, for example: CN201210009971.3 discloses a sobering-up and liver-protecting kudzu root beverage, kudzu root of which is not subjected to enzymolysis, and ketone compounds are expected to be lower and the effect is not obvious.

In order to solve the problems, the invention provides an enzymolysis radix puerariae sobering composite sheet and a preparation method thereof.

Disclosure of Invention

The invention is realized by the following technical scheme:

the enzymolysis radix puerariae composite tablet for sobering up and the preparation method thereof comprise the following components: sorbitol, semen Hoveniae, enzymolysis radix Puerariae, semen Maydis oligopeptide, magnesium stearate, and stevioside.

Preferably, the formula consists of the following components in parts by weight: 10-20 parts of sorbitol, 5-10 parts of semen hoveniae, 6-10 parts of enzymolysis radix puerariae, 3-5 parts of corn oligopeptide, 0.1-0.2 part of magnesium stearate and 0.1-0.15 part of stevioside.

The enzymolysis radix puerariae is prepared by the following steps:

(1) uniformly spraying the multienzyme combined solution A on the surface of fresh radix Puerariae picked within 3 days according to the dosage of 100mL/kg, compacting, and acting in an oxygen-free closed environment for 7 days;

(2) squeezing for dewatering, and hot air drying at 80 deg.C for 60 min until the water content is less than or equal to 15% to obtain processed radix Puerariae;

(3) adding dehydrated radix Puerariae into 1kg of ethanol 3-5L, performing static extraction at room temperature for 24 hr, and collecting extractive solution;

(4) concentrating the extract liquid by a vacuum rotary dryer at 60 ℃, 0.03MPa and 30rpm until no distillate exists;

(5) adding deionized water into the radix puerariae extract obtained in the step (4) according to the volume ratio of 1:2, and adding the multienzyme combined solution B into the mixed solution again according to the proportion of 10ml to 1L; reacting at 45 ℃ and 120rpm for 24 hours;

(6) standing for 12 hr, separating water phase and organic phase, removing water phase, collecting organic phase, spray drying, and granulating to obtain zymolytic radix Puerariae.

The multienzyme combined solution A consists of the following enzymes in parts by weight: 20 parts of amylase, 10 parts of phosphatase, 5 parts of endo beta-glucanase, 18 parts of pectinase, 10 parts of cutinase, 7 parts of protease and 5000 parts of distilled water.

The solution B of the multienzyme combined solution is prepared from the following enzymes in parts by weight: 10 parts of endoxylanase, 10 parts of cellulase, 6 parts of phosphatase, 5 parts of cutinase, 7 parts of protease and 5000 parts of distilled water.

Application of the radix puerariae sobering composite tablet in sobering up.

The invention has the advantages of

(1) The multi-enzyme composition solution A is applied to fresh radix Puerariae to maintain its daidzein concentration, degrade starch, and prevent respiration of daidzein, and the multi-enzyme composition solution B is added to radix Puerariae extractive solution to further concentrate daidzein concentration.

(2) The enzymolysis radix puerariae prepared by the process has a good inhibition effect on headache generated after drinking.

Detailed Description

The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples are commercially available unless otherwise specified.

Example 1

The formula is as follows: 15 parts of sorbitol, 8 parts of semen hoveniae, 8 parts of enzymolysis radix puerariae, 4 parts of corn oligopeptide, 0.1 part of magnesium stearate and 0.1 part of stevioside.

The preparation method comprises the following steps:

(1) uniformly spraying the multienzyme combined solution A on the surface of fresh radix Puerariae picked within 3 days according to the dosage of 100mL/kg, compacting, and acting in an oxygen-free closed environment for 7 days;

(2) squeezing for dewatering, and hot air drying at 80 deg.C for 60 min until the water content is less than or equal to 15% to obtain processed radix Puerariae;

(3) adding dehydrated radix Puerariae into 1kg of ethanol 3L, performing static extraction at room temperature for 24 hr, and collecting extractive solution;

(4) concentrating the extract liquid by a vacuum rotary dryer at 60 ℃, 0.03MPa and 30rpm until no distillate exists;

(5) adding deionized water into the radix puerariae extract obtained in the step (4) according to the volume ratio of 1:2, and adding the multienzyme combined solution B into the mixed solution again according to the proportion of 10ml to 1L; reacting at 45 ℃ and 120rpm for 24 hours;

(6) standing for 12 hr, separating water phase and organic phase, removing water phase, collecting organic phase, spray drying, and granulating to obtain zymolytic radix Puerariae.

The multienzyme combined solution A consists of the following enzymes in parts by weight: 20 parts of amylase, 10 parts of phosphatase, 5 parts of endo-beta-glucanase, 18 parts of pectinase, 10 parts of cutinase, 7 parts of protease and 3000 parts of distilled water.

The solution B of the multienzyme combined solution is prepared from the following enzymes in parts by weight: 10 parts of endoxylanase, 10 parts of cellulase, 6 parts of phosphatase, 5 parts of cutinase, 7 parts of protease and 3000 parts of distilled water.

Mixing and crushing 15 parts of sorbitol, 8 parts of semen hoveniae, 8 parts of enzymolysis radix puerariae, 4 parts of corn oligopeptide, 0.1 part of magnesium stearate and 0.1 part of stevioside to 150 meshes, drying and tabletting to obtain the corn-soybean milk.

Example 2

The formula is as follows: 15 parts of sorbitol, 8 parts of hovenia dulcis thunb, 8 parts of kudzuvine root, 4 parts of corn oligopeptide, 0.1 part of magnesium stearate and 0.1 part of stevioside.

The preparation method comprises the following steps: mixing above materials, pulverizing into 150 granules, drying, and tabletting.

The tabletted candies obtained from examples 1 and 2 were analyzed and tested three times by recruiting 100 volunteers, each of whom was the same, and drunk the same quality of white spirit of the same brand, and were hangover in 23: 00, examples 1, 2 and blank controls were taken before bedtime. The blood alcohol content in the hangover human body is more than 100mg/100ml, and the morning is 7: 00 challenge tests and data collection were performed on 100 persons, and a control blank was only drinking purified water. The results are shown in Table 1 below.

TABLE 1

From the above data, it can be seen that the pueraria lobata agent of the present invention can effectively prevent headache caused after hangover.