阅读说明：本技术 一种氨基酸液的制备方法、氨基酸液、氨基酸水溶肥及其应用 (preparation method of amino acid liquid, amino acid water-soluble fertilizer and application thereof ) 是由 钱潘攀 翟修彩 王国玲 杨风顺 李永泉 李肖宇 林克明 陈晓燕 骆毛喜 于 2019-08-26 设计创作，主要内容包括：本发明提供了一种氨基酸液的制备方法、氨基酸液、氨基酸水溶肥及其应用,涉及农业肥料制造技术领域,所述制备方法,包括以下步骤：(1)将脱脂豆粕用水进行提取,得到大豆蛋白提取液；所述脱脂豆粕与水的质量比为1:(25～35)；(2)将所述步骤(1)得到的大豆蛋白提取液与枯草芽孢杆菌发酵液混合进行发酵,得到氨基酸液；所述枯草芽孢杆菌发酵液的用量占大豆蛋白提取液总体积的5％～10％；所述枯草芽孢杆菌发酵液中的有效活菌数≥2亿/mL。利用本发明的制备方法使得氨基酸的转化率达到45％以上,游离氨基酸含量达到100g/L以上。(The invention provides a preparation method of amino acid liquid, the amino acid liquid, an amino acid water-soluble fertilizer and application thereof, and relates to the technical field of agricultural fertilizer manufacturing, wherein the preparation method comprises the following steps: (1) extracting the defatted soybean meal with water to obtain a soybean protein extracting solution; the mass ratio of the defatted soybean meal to water is 1 (25-35); (2) mixing the soybean protein extracting solution obtained in the step (1) with bacillus subtilis fermentation liquor for fermentation to obtain amino acid liquor; the using amount of the bacillus subtilis fermentation liquor accounts for 5-10% of the total volume of the soybean protein extracting solution; the number of effective viable bacteria in the bacillus subtilis fermentation liquor is more than or equal to 2 hundred million/mL. The preparation method of the invention can ensure that the conversion rate of the amino acid reaches more than 45 percent and the content of the free amino acid reaches more than 100 g/L.)

1. the preparation method of the amino acid solution is characterized by comprising the following steps:

(1) Extracting the defatted soybean meal with water to obtain a soybean protein extracting solution; the mass ratio of the defatted soybean meal to water is 1 (25-35);

(2) mixing the soybean protein extracting solution obtained in the step (1) with bacillus subtilis fermentation liquor for fermentation to obtain amino acid liquor; the using amount of the bacillus subtilis fermentation liquor accounts for 5-10% of the total volume of the soybean protein extracting solution; the number of effective viable bacteria in the bacillus subtilis fermentation liquor is more than or equal to 2 hundred million/mL.

2. The method according to claim 1, wherein the pH value extracted in the step (1) is 7 to 12.

3. The method according to claim 1, wherein the temperature of the extraction in the step (1) is 40 to 80 ℃ and the extraction time is 50 to 100 min.

4. The method according to claim 3, wherein the extraction in step (1) is performed by microwave extraction.

5. The method according to claim 4, wherein the microwave extraction power is 300-1000W.

6. The preparation method according to claim 1, wherein the temperature of the fermentation in the step (2) is 25-30 ℃ and the time of the fermentation is 24-72 h.

7. An amino acid solution produced by the production method according to any one of claims 1 to 6.

8. An amino acid water-soluble fertilizer, characterized by being prepared from the amino acid solution of claim 7 and trace elements.

9. The amino acid water-soluble fertilizer as claimed in claim 8, wherein the trace elements comprise one or more of ferrous sulfate heptahydrate, zinc sulfate heptahydrate, boric acid, sodium iron ethylenediaminetetraacetate and sodium zinc ethylenediaminetetraacetate.

10. use of the amino acid water-soluble fertilizer of claim 8 or 9 for increasing crop yield.

Technical Field

the invention relates to the technical field of agricultural fertilizer manufacturing, in particular to a preparation method of amino acid liquid, the amino acid liquid, an amino acid water-soluble fertilizer and application thereof.

Background

Due to continuous cropping planting of people in successive years, unreasonable use of chemical fertilizers for a long time and multiple flushing application of macroelement water-soluble fertilizers, severe soil hardening, soil acidification and soil salinization are caused to occur, increasingly severe soil fertility reduction and occurrence of soil-borne diseases are caused, and finally crop quality is reduced.

The resources such as bean pulp and the like are rich in amino acid, have wide sources and low cost, but do not achieve high-value utilization to a certain extent.

Disclosure of Invention

In view of the above, the invention aims to provide an amino acid solution, a preparation method of the amino acid solution, an amino acid water-soluble fertilizer and an application of the amino acid water-soluble fertilizer. The amino acid conversion rate in the amino acid solution prepared by the preparation method provided by the invention reaches more than 45%, and the content of free amino acid reaches more than 100 g/L.

In order to achieve the above object, the present invention provides the following technical solutions:

The invention provides a preparation method of amino acid liquid, which comprises the following steps:

(1) Extracting the defatted soybean meal with water to obtain a soybean protein extracting solution; the mass ratio of the defatted soybean meal to water is 1 (25-35);

(2) mixing the soybean protein extracting solution obtained in the step (1) with bacillus subtilis fermentation liquor for fermentation to obtain amino acid liquor; the using amount of the bacillus subtilis fermentation liquor accounts for 5-10% of the total volume of the soybean protein extraction liquid; the number of effective viable bacteria in the bacillus subtilis fermentation liquor is more than or equal to 2 hundred million/mL.

Preferably, the pH value extracted in the step (1) is 7-12.

Preferably, the extraction temperature in the step (1) is 40-80 ℃, and the extraction time is 50-100 min.

Preferably, the extraction in step (1) is microwave extraction.

Preferably, the power of microwave extraction is 300-1000W.

Preferably, the fermentation temperature in the step (2) is 25-30 ℃, and the fermentation time is 24-72 h.

The invention also provides the amino acid solution prepared by the preparation method.

The invention also provides an amino acid water-soluble fertilizer which is prepared from the amino acid liquid and trace elements.

Preferably, the trace elements comprise one or more of ferrous sulfate heptahydrate, zinc sulfate heptahydrate, boric acid, ethylenediamine tetraacetic acid iron sodium and ethylenediamine tetraacetic acid zinc sodium.

The invention also provides application of the amino acid water-soluble fertilizer in the technical scheme in improving crop yield.

The invention provides a preparation method of amino acid liquid, which takes defatted soybean meal as a raw material and utilizes bacillus subtilis rich in protease to carry out fermentation treatment, so that the conversion rate of amino acid reaches more than 45 percent, and the content of free amino acid reaches more than 100 g/L.

The amino acid water-soluble fertilizer provided by the invention can not only provide a nitrogen source for crops, but also promote photosynthesis of crops, regulate crop growth, relieve fertilizer damage and phytotoxicity and relieve toxic effects of heavy metal ions, so that the growth condition of the crops can be effectively improved, and the effect of improving the crop yield is achieved.

Detailed Description

The invention provides a preparation method of amino acid liquid, which comprises the following steps:

(1) extracting the defatted soybean meal with water to obtain a soybean protein extracting solution; the mass ratio of the defatted soybean meal to water is 1 (25-35);

(2) Mixing the soybean protein extracting solution obtained in the step (1) with bacillus subtilis fermentation liquor for fermentation to obtain amino acid liquor; the using amount of the bacillus subtilis fermentation liquor accounts for 5-10% of the total volume of the soybean protein extraction liquid; the number of effective viable bacteria in the bacillus subtilis fermentation liquor is more than or equal to 2 hundred million/mL.

The method comprises the steps of extracting defatted soybean meal with water to obtain a soybean protein extracting solution; the mass ratio of the defatted soybean meal to water is 1 (25-35), and preferably 1: 30. The source of the defatted soybean meal is not particularly limited, and the defatted soybean meal can be obtained by adopting a conventional commercial product.

In the invention, the pH value of the extraction is preferably 7-12, and more preferably 8-9. In the invention, the extraction temperature is preferably 40-80 ℃, more preferably 50-70 ℃, and most preferably 60 ℃; the extraction time is preferably 50-100 min, more preferably 60-80 min, and most preferably 70 min.

In the invention, the extraction method is preferably microwave extraction, and the power of the microwave extraction is preferably 300-1000W, more preferably 600-900W, and most preferably 800W.

In the invention, the soybean protein extracting solution is preferably supernatant obtained by centrifugal impurity removal, and the centrifugal rotating speed is preferably 4000 r/min-6000 r/min, and more preferably 5000 r/min; the centrifugation time is preferably 10-30 min, more preferably 15-25 min, and most preferably 20 min.

Mixing the obtained soybean protein extracting solution with bacillus subtilis fermentation liquor for fermentation to obtain amino acid liquor; the using amount of the bacillus subtilis fermentation liquor accounts for 5-10%, preferably 6-8% of the total volume of the soybean protein extracting solution; the number of effective viable bacteria in the bacillus subtilis fermentation liquor is more than or equal to 2 hundred million/mL. The method for mixed fermentation is not particularly limited, and a conventional mixed fermentation method can be adopted.

In the invention, the Bacillus subtilis is preferably a Sanju-07 strain which is preserved in China general microbiological culture Collection center in 29 days 12 and 29 days 2014, the preservation address is No. 3 of No.1 Xilu Beichen of Chaoyang district in Beijing, and the preservation number is CGMCC No. 10248.

In the invention, the fermentation medium of the bacillus subtilis uses water as a solvent, and each liter of the fermentation medium comprises: 25-35 g of starch, 2-7 g of cane sugar, 1-5 g of monopotassium phosphate, 0.1-0.5 g of manganese sulfate and 0.2-2 g of yeast extract; the pH value of the fermentation medium is preferably 7.0-7.2. In the present invention, the fermentation medium of bacillus subtilis uses water as a solvent, and preferably comprises per liter: 30g of starch, 5g of cane sugar, 3g of monopotassium phosphate, 0.3g of manganese sulfate and 1g of yeast extract.

In the invention, the fermentation temperature is preferably 25-30 ℃, and more preferably 28 ℃; the fermentation time is preferably 24-72 h, more preferably 36-60 h, and most preferably 48 h.

The invention also provides an amino acid water-soluble fertilizer which is prepared from the amino acid liquid and trace elements. In the invention, the trace elements preferably comprise one or more of ferrous sulfate heptahydrate, zinc sulfate heptahydrate, boric acid, sodium iron ethylenediaminetetraacetate and sodium zinc ethylenediaminetetraacetate. In the invention, 20-40 g, more preferably 25-35 g, 70-100 g, more preferably 80-90 g of zinc sulfate heptahydrate, and 10-20 g, more preferably 12-18 g of boric acid are preferably added into 1L of the amino acid solution. The preparation method of the amino acid water-soluble fertilizer is not particularly limited, and the amino acid water-soluble fertilizer is prepared by stirring for 1-4 hours at the temperature of 15-40 ℃ and at the speed of 100-800 r/min; more preferably, the material is prepared by stirring for 2-3 hours at the temperature of 20-30 ℃ and the speed of 400-600 r/min.

The application method of the amino acid water-soluble fertilizer is not particularly limited, and the amino acid water-soluble fertilizer can not be preferably mixed with alkaline pesticide, alkaline fertilizer and phosphate fertilizer for use.

In the invention, the application method of the amino acid water-soluble fertilizer comprises the following steps: and (3) seedling stage: spraying the liquid with the optimal concentration of 0.3-1.0L/mu and 500-1000 times; drip irrigation preferably by 0.5-1.0L/mu and 500-1000 times of liquid; growth period and fruiting period: spraying the solution with the optimal concentration of 0.3-2.0L/mu and 1000-2000 times; the drip irrigation is preferably 0.5-2.0L/mu, 500-1000 times of liquid.

The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without any inventive step, are within the scope of the present invention.