阅读说明：本技术 一种复合多肽材料制备用纯化装置及其加工方法 (Purification device for preparing composite polypeptide material and processing method thereof ) 是由 郑秋芳 叶艳鹏 肖志勇 方成 于 2021-09-07 设计创作，主要内容包括：本发明公开了一种复合多肽材料制备用纯化装置,包括倒T型底座,倒T型底座的上端设有柱形筒,柱形筒靠近环形内壁的底部呈倾斜设置。一种复合多肽材料制备用纯化加工方法,其操作步骤如下：手动将离心桶贯穿环形限位块后插设于柱形筒内。本发明通过第二电机带动倾斜搅拌杆和锥形搅拌板转动,使得倾斜搅拌杆和锥形搅拌板对离心桶内的复合多肽和提纯用反应剂进行搅拌混合,提高反应剂与复合多肽的混合效果,通过将离心桶贯穿环形限位块后插设于柱形筒内,使得矩形限位块贯穿矩形开口设置并插设于环形限位槽内,并使得离心桶插设于圆形槽内,矩形凸起插设于矩形卡槽内,有效对离心桶进行限位固定,方便拆装离心桶后快速取出提纯物质。(The invention discloses a purification device for preparing a composite polypeptide material, which comprises an inverted T-shaped base, wherein a cylindrical barrel is arranged at the upper end of the inverted T-shaped base, and the bottom of the cylindrical barrel, which is close to an annular inner wall, is obliquely arranged. A purification processing method for preparing a composite polypeptide material comprises the following operation steps: the centrifugal barrel is manually inserted into the cylindrical barrel after penetrating through the annular limiting block. According to the invention, the inclined stirring rod and the conical stirring plate are driven to rotate by the second motor, so that the inclined stirring rod and the conical stirring plate stir and mix the composite polypeptide and the purification reactant in the centrifugal barrel, the mixing effect of the reactant and the composite polypeptide is improved, the centrifugal barrel penetrates through the annular limiting block and then is inserted into the cylindrical barrel, the rectangular limiting block penetrates through the rectangular opening and is inserted into the annular limiting groove, the centrifugal barrel is inserted into the circular groove, and the rectangular bulge is inserted into the rectangular clamping groove, so that the centrifugal barrel is effectively limited and fixed, and the purified substances can be conveniently and quickly taken out after the centrifugal barrel is disassembled and assembled.)

1. A purification device for preparing a composite polypeptide material, which comprises an inverted T-shaped base (1), it is characterized in that the upper end of the inverted T-shaped base (1) is provided with a cylindrical barrel (2), the bottom of the cylindrical tube (2) close to the annular inner wall is obliquely arranged, two liquid discharge holes (3) are arranged on the annular inner wall of the cylindrical tube (2) close to the oblique bottom, a cavity (4) is arranged in the inverted T-shaped base (1), a clamping mechanism is vertically arranged on the top surface of the cavity (4) in a penetrating way, a centrifugal barrel (5) is arranged on the clamping mechanism, a limiting mechanism is arranged between the centrifugal barrel (5) and the cylindrical barrel (2), the upper end screw thread of centrifugation bucket (5) has cup jointed annular apron (6), annular apron (6) are located the vertical rabbling mechanism that runs through on the top surface of central point position, the rabbling mechanism is close to the bottom setting of cylindricality section of thick bamboo (2).

2. The purification device for preparing a composite polypeptide material according to claim 1, wherein the clamping mechanism comprises a first motor (7) fixedly mounted on the bottom of the chamber (4), a driving shaft of the first motor (7) is vertically arranged upwards, a connecting rod (8) is fixedly connected to the upper end of the driving shaft of the first motor (7), the upper end of the connecting rod (8) vertically rotates to penetrate through the top surface of the central position of the chamber (4), a round block (9) is fixedly connected to the upper end of the connecting rod (8), a round groove (10) is formed in the upper end of the round block (9), two rectangular protrusions (11) are arranged on the bottom of the round groove (10), two rectangular clamping grooves (12) are formed in the lower end of the centrifugal barrel (5), and the lower end of the centrifugal barrel (5) is inserted into the round groove (10), the two rectangular bulges (11) are respectively inserted into the two rectangular clamping grooves (12).

3. The purification device for preparing composite polypeptide material according to claim 2, wherein the rectangular protrusion (11) and the circular block (9) are integrally arranged, the bottom of the horizontal section of the cylindrical barrel (2) is fixedly connected with an annular waterproof ring (13), the lower end of the circular block (9) is in contact with the upper end of the annular waterproof ring (13) in an abutting manner, and the annular waterproof ring (13) is made of wear-resistant rubber.

4. The purification device for preparing a composite polypeptide material according to claim 1, wherein the limiting mechanism comprises two symmetrically distributed rectangular limiting blocks (14) fixedly connected to the annular side wall of the centrifugal barrel (5) and an annular limiting block (15) fixedly connected to the annular inner wall of the cylindrical barrel (2), the two rectangular limiting blocks (14) are both arranged near the upper end of the centrifugal barrel (5), an annular limiting groove (16) is arranged on the annular inner wall of the annular limiting block (15), two symmetrically distributed rectangular openings (17) are arranged on the top surface of the annular limiting groove (16), and the two rectangular limiting blocks (14) are respectively arranged through the two rectangular openings (17) and inserted into the annular limiting groove (16).

5. The purification device for preparing the composite polypeptide material as claimed in claim 1, wherein the annular inner wall of the annular cover plate (6) is provided with an internal thread (18), the annular side wall of the centrifugal barrel (5) near the upper end is provided with an external thread (19), the internal thread (18) is in threaded connection with the external thread (19), the top surface of the annular cover plate (6) is fixedly connected with an annular sealing ring (20), the lower end of the annular sealing ring (20) is in contact with the upper end of the centrifugal barrel (5) in an abutting manner, and the upper end of the annular cover plate (6) is fixedly connected with two handles (21).

6. The purification device is used in preparation of composite polypeptide material according to claim 1, wherein the stirring mechanism includes a second motor (22) fixedly mounted on the annular cover plate (6) and located at the upper end of the central position, the drive shaft of the second motor (22) is vertically arranged downwards, the lower end of the drive shaft of the second motor (22) is fixedly connected with a rotating rod (23), the lower end of the rotating rod (23) is vertically rotated to penetrate through the annular cover plate (6) and extend into the centrifuge bucket (5), the rotating rod (23) is located on the annular side wall in the centrifuge bucket (5) and is fixedly connected with a plurality of inclined stirring rods (24) and two conical stirring plates (25) which are distributed in equal intervals, and the lower ends of the conical stirring plates (25) are both arranged near the bottom of the centrifuge bucket (5).

7. A method for processing a purification device for preparing a composite polypeptide material according to any one of claims 1 to 6, comprising the following steps:

step S1: the centrifugal barrel (5) is manually inserted into the cylindrical barrel (2) after penetrating through the annular limiting block (15), the centrifugal barrel (5) is rotated to enable the two rectangular limiting blocks (14) to respectively penetrate through the two rectangular openings (17) in the annular limiting block (15) and be inserted into the annular limiting groove (16), the lower end of the centrifugal barrel (5) is inserted into the circular groove (10) in the circular block (9), the two rectangular bulges (11) in the circular groove (10) are respectively inserted into the two rectangular clamping grooves (12) at the lower end of the centrifugal barrel (5), and the centrifugal barrel (5) is effectively limited and fixed;

step S2: introducing the compound polypeptide to be purified and a reactant for purification into a centrifugal barrel (5), holding a handle (21) to cover an annular cover plate (6) at the upper end of the centrifugal barrel (5), inserting a stirring mechanism into the centrifugal barrel (5), rotating the annular cover plate (6) to enable an internal thread (18) to be in threaded connection with an external thread (19) until an annular sealing ring (20) is abutted against the upper end of the centrifugal barrel (5);

step S3: a second motor (22) is started to drive a plurality of inclined stirring rods (24) and two conical stirring plates (25) on the rotating rod (23) to rotate, so that the inclined stirring rods (24) are matched with the conical stirring plates (25) to stir and mix the composite polypeptide and the purification reactant in the centrifugal barrel (5);

step S4: after the second motor (22) is turned off, the first motor (7) is started, the first motor (7) is matched with the connecting rod (8) and the round block (9) to drive the centrifugal barrel (5) to rotate, the centrifugal barrel (5) carries out centrifugal purification on the reacted composite polypeptide, the centrifuged liquid is discharged from the liquid discharge hole (3) along the inclined bottom of the cylindrical barrel (2), and the purified composite polypeptide is retained in the centrifugal barrel (5).

Technical Field

The invention relates to the technical field of composite polypeptide purification, in particular to a purification device for preparing a composite polypeptide material and a processing method thereof.

Background

The composite polypeptide is a compound formed by connecting alpha amino acids together by a peptide chain, the composite polypeptide is also an intermediate product of proteolysis, the composite polypeptide is one of the most basic and important substances forming life, in fact, active biological composite polypeptides are widely distributed and exist in nature, a lot of bioactive substances in organisms mostly exist in the form of polypeptide structures, and the composite polypeptide can penetrate through a semipermeable membrane and is not precipitated by trichloroacetic acid and ammonium sulfate.

When the existing purification device for producing the composite polypeptide is actually used, the mixing effect of a reactant for purification and the composite polypeptide is poor, and a purified substance is not convenient to take out quickly, so that the purification device for preparing the composite polypeptide material and the processing method thereof are provided to solve the problems.

Disclosure of Invention

The invention provides a purification device for preparing a composite polypeptide material and a processing method thereof, which are used for solving the technical problems that in the prior art, when the existing purification device for producing the composite polypeptide is actually used, the mixing effect of a reactant for purification and the composite polypeptide is poor, and the purified substances are not convenient to take out quickly.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a composite polypeptide material is purification device for preparation, includes the type of falling T base, the upper end of the type of falling T base is equipped with a cylindricality section of thick bamboo, the bottom that the cylindricality section of thick bamboo is close to annular inner wall is the slope setting, the cylindricality section of thick bamboo is equipped with two outage on being close to the annular inner wall of slope bottom, be equipped with the cavity in the type of falling T base, the vertical joint mechanism that runs through on the top surface of cavity, the last centrifugal bucket that is equipped with of joint mechanism, be equipped with stop gear between centrifugal bucket and the cylindricality section of thick bamboo, the upper end screw thread of centrifugal bucket has cup jointed annular apron, the annular apron is located the vertical rabbling mechanism that runs through on the top surface of central point, rabbling mechanism is close to the bottom setting of a cylindricality section of thick bamboo.

Preferably, the clamping mechanism includes the first motor of fixed mounting on the cavity bottom, the drive shaft of first motor is vertical to be set up, the upper end fixedly connected with connecting rod of first motor drive shaft, the vertical top surface setting that runs through the cavity and be located central point and put of upper end rotation of connecting rod, the upper end fixedly connected with circular block of connecting rod, the upper end of circular block is equipped with the circular recess, be equipped with two rectangle archs on the bottom of circular recess, the lower extreme of centrifugal barrel is equipped with two rectangle draw-in grooves, the lower extreme of centrifugal barrel is inserted and is located the circular recess, two the rectangle arch is inserted respectively and is located in two rectangle draw-in grooves.

Preferably, the rectangle arch is the integration setting with the circular block, fixedly connected with annular waterproof circle on the horizontal segment bottom of cylindricality section of thick bamboo, the lower extreme of circular block and the upper end looks butt contact of annular waterproof circle, annular waterproof circle is wear-resisting rubber goods.

Preferably, the limiting mechanism comprises two rectangular limiting blocks which are symmetrically distributed and two annular limiting blocks which are fixedly connected to the annular side wall of the centrifugal barrel respectively and fixedly connected to the annular inner wall of the cylindrical barrel, the two rectangular limiting blocks are arranged close to the upper end of the centrifugal barrel, an annular limiting groove is formed in the annular inner wall of each annular limiting block, two rectangular openings which are symmetrically distributed are formed in the top surface of the annular limiting groove, and the two rectangular limiting blocks penetrate through the two rectangular openings respectively and are inserted into the annular limiting groove.

Preferably, be equipped with the internal thread on the annular inner wall of annular apron, the annular lateral wall that the centrifugation bucket is close to the upper end is equipped with the external screw thread, internal thread and external screw thread threaded connection, fixedly connected with ring packing circle on the top surface of annular apron, ring packing circle's lower extreme and centrifugation bucket's upper end counterbalance contact, two handles of ring packing circle's upper end fixedly connected with.

Preferably, rabbling mechanism includes that fixed mounting is located the second motor of central point position upper end in annular apron, the drive shaft of second motor sets up down vertically, the lower extreme fixedly connected with dwang of second motor drive shaft, the lower extreme vertical rotation of dwang runs through annular apron setting and extends to the centrifuge bucket in, the dwang is located slope puddler and two toper stirring boards that fixedly connected with distributes between a plurality of equidistance on the annular lateral wall in the centrifuge bucket, two the lower extreme of toper stirring board all is close to the bottom setting of centrifuge bucket.

A purification processing method for preparing a composite polypeptide material comprises the following operation steps:

step S1: the centrifugal barrel is manually inserted into the cylindrical barrel after penetrating through the annular limiting block, the centrifugal barrel is rotated to enable the two rectangular limiting blocks to respectively penetrate through the two rectangular openings in the annular limiting block and be inserted into the annular limiting groove, the lower end of the centrifugal barrel is inserted into the circular groove in the circular block at the moment, and the two rectangular bulges in the circular groove are respectively inserted into the two rectangular clamping grooves in the lower end of the centrifugal barrel, so that the centrifugal barrel is effectively limited and fixed;

step S2: introducing the compound polypeptide to be purified and a reactant for purification into a centrifugal barrel, holding a handle to cover an annular cover plate at the upper end of the centrifugal barrel, inserting a stirring mechanism into the centrifugal barrel, and rotating the annular cover plate to enable internal threads to be in threaded connection with external threads until an annular sealing ring is abutted against the upper end of the centrifugal barrel;

step S3: starting a second motor to drive a plurality of inclined stirring rods and two conical stirring plates on the rotating rod to rotate, so that the inclined stirring rods are matched with the conical stirring plates to stir and mix the composite polypeptide and the purification reactant in the centrifugal barrel;

step S4: close after the second motor and start first motor for first motor cooperation connecting rod and circular block drive the centrifugation bucket and rotate, make the centrifugation bucket carry out centrifugal purification to the compound polypeptide after the reaction, and liquid after the centrifugation is discharged via the outage along the slope bottom of cylindricality section of thick bamboo, and the compound polypeptide after the purification is detained in the centrifugation bucket.

Compared with the prior art, the invention has the beneficial effects that:

1. the inclined stirring rod and the conical stirring plate are driven to rotate through the second motor, so that the inclined stirring rod and the conical stirring plate stir and mix the composite polypeptide in the centrifugal barrel and the purification reactant, and the mixing effect of the reactant and the composite polypeptide is improved.

2. Insert after running through annular stopper through the centrifugation bucket and locate cylindricality section of thick bamboo for the rectangle stopper runs through the rectangle opening and sets up and insert and locate annular spacing inslot, and makes the centrifugation bucket insert and locate in the circular recess, and the rectangle arch is inserted and is located the rectangle draw-in groove, effectively carries out spacing fixed to the centrifugation bucket, takes out the purification material behind the convenient dismouting centrifugation bucket fast.

Drawings

FIG. 1 is a schematic structural diagram of a purification apparatus for preparing a composite polypeptide material according to the present invention;

FIG. 2 is a schematic perspective view of a purification apparatus for preparing a composite polypeptide material according to the present invention;

FIG. 3 is a schematic structural diagram of an annular waterproof ring of a purification device for preparing a composite polypeptide material, which is provided by the invention;

FIG. 4 is an enlarged view of a portion of FIG. 2 at A;

FIG. 5 is an enlarged view of a portion of FIG. 2 at B;

FIG. 6 is a schematic structural diagram of an annular limiting block of the purification device for preparing a composite polypeptide material according to the present invention;

fig. 7 is a schematic structural diagram of a stirring mechanism of a purification apparatus for preparing a composite polypeptide material according to the present invention.

In the figure: the stirring device comprises a 1-turn-T-shaped base, a 2-column-shaped cylinder, a 3-liquid discharge hole, a 4-cavity chamber, a 5-centrifugal barrel, a 6-annular cover plate, a 7-first motor, 8-connecting rods, 9-round blocks, 10-round grooves, 11-rectangular protrusions, 12-rectangular clamping grooves, 13-annular waterproof rings, 14-rectangular limiting blocks, 15-annular limiting blocks, 16-annular limiting grooves, 17-rectangular openings, 18-internal threads, 19-external threads, 20-annular sealing rings, 21 handles, 22-second motors, 23-rotating rods, 24-inclined stirring rods and 25-conical stirring plates.

Detailed Description

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-7, a composite polypeptide material is purification device for preparation, including type of falling T base 1, type of falling T base 1 is used for supporting whole device, type of falling T base 1's upper end is equipped with a cylindricality section of thick bamboo 2, cylindricality section of thick bamboo 2 is the slope setting near the bottom of annular inner wall, cylindricality section of thick bamboo 2 is equipped with two outage 3 near the annular inner wall of slope bottom, outage 3 are used for deriving the liquid after the centrifugation, be equipped with cavity 4 in the type of falling T base 1, the vertical latch mechanism that is equipped with that runs through on cavity 4's the top surface, the last centrifugal bucket 5 that is equipped with of latch mechanism, centrifugal bucket 5 is used for loading reactant and composite polypeptide, latch mechanism includes first motor 7 of fixed mounting on cavity 4 bottom, the model of first motor 7 is: S370Y22L, a first motor 7 is used for driving a connecting rod 8 and a circular block 9 to rotate, a driving shaft of the first motor 7 is vertically arranged upwards, the upper end of the driving shaft of the first motor 7 is fixedly connected with the connecting rod 8, the upper end of the connecting rod 8 vertically rotates to penetrate through the top surface of a central position of a chamber 4, the upper end of the connecting rod 8 is fixedly connected with the circular block 9, the upper end of the circular block 9 is provided with a circular groove 10, the bottom of the circular groove 10 is provided with two rectangular bulges 11, the lower end of a centrifugal barrel 5 is provided with two rectangular clamping grooves 12, the lower end of the centrifugal barrel 5 is inserted into the circular groove 10, the two rectangular bulges 11 are respectively inserted into the two rectangular clamping grooves 12, the rectangular bulges 11 and the circular block 9 are integrally arranged, when the rectangular bulges 11 are inserted into the rectangular clamping grooves 12, the centrifugal barrel 5 is limited and fixed by matching with a rectangular limiting block 14 in the annular limiting groove 16, an annular waterproof ring 13 is fixedly connected on the bottom of a horizontal section of a cylindrical barrel 2, the lower extreme of circular piece 9 offsets with the upper end of annular waterproof circle 13 and contacts, and annular waterproof circle 13 is wear-resisting rubber product, and wear-resisting rubber product's annular waterproof circle 13 can prevent that the centrifugal liquid from flowing to the bottom that cylindrical barrel 2 is located central point and put.

Be equipped with stop gear between centrifuge drum 5 and the cylindricality section of thick bamboo 2, stop gear includes that two blocks that are the symmetric distribution on 5 annular lateral walls of centrifuge drum are rectangle stopper 14 and annular stopper 15 of fixed connection on 2 annular inner walls of cylindricality section of thick bamboo respectively, two rectangle stoppers 14 all are close to the upper end setting of centrifuge drum 5, be equipped with annular spacing groove 16 on the annular inner wall of annular stopper 15, be equipped with two rectangle openings 17 that are the symmetric distribution on the top surface of annular spacing groove 16, two rectangle stoppers 14 run through two rectangle openings 17 respectively and set up and insert and locate annular spacing groove 16, insert after rectangle stopper 14 runs through rectangle opening 17 and locate annular spacing groove 16, when circular piece 9 drives centrifuge drum 5 and rotates, centrifuge drum 5 drives rectangle stopper 14 at the internal rotation of annular spacing groove 16, effectively carry on spacingly to centrifuge drum 5.

The upper end screw thread of centrifugation bucket 5 has cup jointed annular cover plate 6, be equipped with internal thread 18 on the annular inner wall of annular cover plate 6, be equipped with external screw thread 19 on the annular lateral wall that centrifugation bucket 5 is close to the upper end, internal thread 18 and external screw thread 19 threaded connection, when internal thread 18 and external screw thread 19 threaded connection, effectively fix annular cover plate 6 spacing on centrifugation bucket 5, fixedly connected with ring seal 20 on the top surface of annular cover plate 6, ring seal 20's lower extreme and centrifugation bucket 5's upper end counterbalance contact, ring seal 20 cooperation annular cover plate 6 seals centrifugation bucket 5, two handles 21 of upper end fixedly connected with of annular cover plate 6, handle 21 conveniently operates annular cover plate 6 and rabbling mechanism.

Annular apron 6 is located the vertical rabbling mechanism that runs through on the top surface that the central point put, and the rabbling mechanism is close to the bottom setting of cylindricality section of thick bamboo 2, and the rabbling mechanism includes that fixed mounting is in annular apron 6 second motor 22 that is located the central point and puts the upper end, and second motor 22's model is: Y80M1-2, second motor 22 is used for driving a plurality of slopes puddler 24 and two toper stirring boards 25 on the dwang 23 and rotates, make slope puddler 24 cooperate toper stirring board 25 to stir the mixture with the reactant to the compound polypeptide in the centrifuge bucket 5 with purification, improve the mixed effect of reactant and compound polypeptide, the drive shaft of second motor 22 is vertical to be set up down, the lower extreme fixedly connected with dwang 23 of second motor 22 drive shaft, the vertical rotation of lower extreme of dwang 23 runs through annular apron 6 and sets up and extend to in the centrifuge bucket 5, dwang 23 is located the slope puddler 24 and two toper stirring boards 25 that fixedly connected with distributes between a plurality of on the annular lateral wall in centrifuge bucket 5, the lower extreme of two toper stirring boards 25 all is close to the bottom setting of centrifuge bucket 5.

A purification processing method for preparing a composite polypeptide material comprises the following operation steps:

step S1: the centrifugal barrel 5 is manually inserted into the cylindrical barrel 2 after penetrating through the annular limiting block 15, the centrifugal barrel 5 is rotated to enable the two rectangular limiting blocks 14 to respectively penetrate through the two rectangular openings 17 on the annular limiting block 15 and be inserted into the annular limiting groove 16, the lower end of the centrifugal barrel 5 is inserted into the circular groove 10 on the circular block 9 at the moment, and the two rectangular bulges 11 in the circular groove 10 are respectively inserted into the two rectangular clamping grooves 12 at the lower end of the centrifugal barrel 5, so that the centrifugal barrel 5 is effectively limited and fixed;

step S2: introducing the compound polypeptide to be purified and a reactant for purification into a centrifugal barrel 5, holding a handle 21 to cover an annular cover plate 6 on the upper end of the centrifugal barrel 5, inserting a stirring mechanism into the centrifugal barrel 5, and rotating the annular cover plate 6 to enable an internal thread 18 to be in threaded connection with an external thread 19 until an annular sealing ring 20 is abutted against the upper end of the centrifugal barrel 5;

step S3: the second motor 22 is started to drive the plurality of inclined stirring rods 24 and the two conical stirring plates 25 on the rotating rod 23 to rotate, so that the inclined stirring rods 24 are matched with the conical stirring plates 25 to stir and mix the composite polypeptide and the purification reactant in the centrifugal barrel 5;

step S4: close behind the second motor 22 and start first motor 7 for first motor 7 cooperates connecting rod 8 and round piece 9 to drive centrifuge bucket 5 and rotates, makes centrifuge bucket 5 carry out centrifugal purification to the compound polypeptide after the reaction, and liquid after the centrifugation is discharged via outage 3 along the slope bottom of cylindricality section of thick bamboo 2, and the compound polypeptide after the purification is detained in centrifuge bucket 5.

When the device is used, the centrifugal barrel 5 is manually inserted into the cylindrical barrel 2 after penetrating the annular limiting block 15, the centrifugal barrel 5 is rotated to enable the two rectangular limiting blocks 14 to respectively penetrate the two rectangular openings 17 on the annular limiting block 15 and be inserted into the annular limiting groove 16, the lower end of the centrifugal barrel 5 is inserted into the circular groove 10 on the circular block 9, the two rectangular bulges 11 in the circular groove 10 are respectively inserted into the two rectangular clamping grooves 12 at the lower end of the centrifugal barrel 5, the centrifugal barrel 5 is effectively limited and fixed, the compound polypeptide to be purified and the reactant for purification are led into the centrifugal barrel 5, the handle 21 is held to cover the annular cover plate 6 at the upper end of the centrifugal barrel 5, the stirring mechanism is inserted into the centrifugal barrel 5, the annular cover plate 6 is rotated to enable the internal thread 18 to be in threaded connection with the external thread 19 until the annular sealing ring 20 is abutted against the upper end of the centrifugal barrel 5, the second motor 22 is started to drive the inclined stirring rods 24 and the two conical stirring plates 25 on the rotating rod 23 to rotate, so that the inclined stirring rods 24 are matched with the conical stirring plates 25 to stir and mix the composite polypeptide and the purification reactant in the centrifugal barrel 5, the mixing effect of the reactant and the composite polypeptide is improved, the first motor 7 is started after the second motor 22 is closed, the first motor 7 is matched with the connecting rod 8 and the circular block 9 to drive the centrifugal barrel 5 to rotate, the centrifugal barrel 5 is used for centrifugally purifying the reacted composite polypeptide, the centrifuged liquid is discharged from the liquid discharge hole 3 along the inclined bottom of the cylindrical barrel 2, the purified composite polypeptide is retained in the centrifugal barrel 5, after the first motor 7 is closed, the annular cover plate 6 is rotated by using the handle 21 to separate the internal thread 18 from the external thread 19, and the annular cover plate 6 and the stirring mechanism thereof are drawn out of the centrifugal barrel 5, the centrifugal barrel 5 is rotated to enable the rectangular limiting block 14 to move to the rectangular opening 17, the centrifugal barrel 5 is pulled to enable the rectangular protrusion 11 to be separated from the rectangular clamping groove 12 until the rectangular limiting block 14 is drawn out from the annular limiting groove 16 through the rectangular opening 17, and purified materials can be taken out quickly after the centrifugal barrel 5 is assembled and disassembled conveniently.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.