阅读说明：本技术 分离缺少能够结合至蛋白A的Fc区域的抗体或抗体片段的方法 (Method for isolating antibodies or antibody fragments lacking an Fc region capable of binding to protein A ) 是由 G·罗德里戈 M·安德尔 T·比约克曼 R·帕姆格伦 C·布林克 于 2020-04-29 设计创作，主要内容包括：本发明公开分离抗体或抗体片段的方法,其包含以下步骤：a)提供包含具有VH3区域且缺少能够结合至蛋白A的Fc区域的抗体或抗体片段的进料；b)将进料与具有共价偶联配体的分离树脂接触,其中配体包含如SEQ ID NO 1所定义的多肽且其中抗体或抗体片段结合至分离树脂；c)可选地用清洗液清洗分离树脂；d)用洗脱液从分离树脂中洗脱该抗体或抗体片段并回收该抗体或抗体片段。(The present invention discloses a method for isolating an antibody or antibody fragment comprising the steps of: a) providing a feed comprising an antibody or antibody fragment having a VH3 region and lacking an Fc region capable of binding to protein a; b) contacting the feed with a separation resin having a covalently coupled ligand, wherein the ligand comprises a polypeptide as defined in SEQ ID NO 1 and wherein the antibody or antibody fragment binds to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibody or antibody fragment from the separation resin with an eluent and recovering the antibody or antibody fragment.)