阅读说明：本技术 一种解酒组合物、制备方法及其应用 (Anti-alcohol composition, preparation method and application thereof ) 是由 郜海燕 陈杭君 高原 牛犇 刘瑞玲 吴伟杰 房祥军 于 2021-09-14 设计创作，主要内容包括：本发明提供一种解酒组合物、制备方法及其应用。本发明的解酒组合物按质量百分含量包括以下组分：茭白提取物20％～60％；蓝莓提取物20％～60％；辅料10％～30％；营养补充剂1％～20％。该解酒组合物不仅可以用于饮酒后进行解酒,而且可以日常饮用,具有抗氧化、提高免疫力、护肝的功效,该解酒组合物安全性高,无副作用。(The invention provides an anti-alcohol composition, a preparation method and application thereof. The anti-alcohol composition comprises the following components in percentage by mass: 20 to 60 percent of wild rice stem extract; 20-60% of blueberry extract; 10 to 30 percent of auxiliary material; 1 to 20 percent of nutritional supplement. The anti-inebriation composition can be used for performing anti-inebriation after drinking, can be drunk daily, has the effects of resisting oxidation, improving immunity and protecting liver, and has high safety and no side effect.)

1. The anti-alcohol composition is characterized by comprising the following components in percentage by mass:

20 to 60 percent of wild rice stem extract;

20-60% of blueberry extract;

10 to 30 percent of auxiliary material;

1 to 20 percent of nutritional supplement.

2. The hangover-alleviating composition according to claim 1, comprising the following components in percentage by mass:

25 to 45 percent of wild rice stem extract;

25-45% of blueberry extract;

12 to 20 percent of auxiliary material;

1 to 10 percent of nutritional supplement.

3. The anti-hangover composition according to claim 1 or 2, wherein the auxiliary material includes at least one of pueraria extract, lonicera extract, lycium extract, chrysanthemum extract, tsaoko amomum fruit extract, and bambusae caulis extract.

4. The anti-inebriation composition according to claim 3, wherein the auxiliary materials comprise the following components in percentage by mass based on the total mass of the anti-inebriation composition:

2 to 10 percent of kudzu root extract;

1-6% of honeysuckle extract;

1 to 6 percent of medlar extract;

1 to 6 percent of chrysanthemum extract;

1 to 6 percent of tsaoko amomum fruit extract;

1 to 6 percent of bamboo shavings extract.

5. The anti-hangover composition according to any one of claims 1-4, wherein the nutritional supplement comprises at least one of vitamin B, vitamin C, L-cysteine, L-theanine and taurine.

6. The anti-hangover composition according to claim 5, wherein the vitamin B comprises at least one of vitamin B1, vitamin B2, and vitamin B6.

7. A method for preparing the anti-hangover composition according to any one of claims 1 to 6, wherein the anti-hangover composition is obtained by mixing Zizania latifolia extract, blueberry extract, auxiliary materials and nutritional supplements.

8. Use of the anti-hangover composition according to any one of claims 1 to 6 in an anti-hangover health product or an antioxidant health product.

9. A health product comprising the anti-hangover composition according to any one of claims 1 to 6.

10. The health product of claim 9, further comprising at least one of white granulated sugar, xylitol, honey, concentrated fruit juice, fruit powder, citric acid, and malic acid.

Technical Field

The invention relates to an anti-alcohol composition, a preparation method and application thereof, belonging to the field of health-care food.

Background

With the increase of the economic level of people, the consumption of alcohol products tends to increase. According to the world health organization reports, 2.37 million men and 4600 million women are affected by unhealthy drinking. Acute alcoholism is also called drunkenness, and is mainly caused by that after drinking too much in a short time, absorbed ethanol can not be metabolized in the liver in time and enters blood and brain; meanwhile, a large amount of acetaldehyde which is a metabolic intermediate product of part of ethanol is accumulated, a nervous system and a digestive system are stimulated, and a plurality of adverse reactions such as heartbeat acceleration, light headedness, slurred speech, gastrointestinal discomfort and the like are generated.

Most of the products for treating alcoholism in the current market are synthetic drugs, such as naloxone, US RU21, metadoxine and the like. Although these drugs can temporarily relieve the symptoms of alcohol intoxication and have anti-hangover effects, they have limited effects on relieving a series of symptoms caused by alcohol. With the increasing demand of people for healthy life, the development trend of anti-alcohol products gradually tends to natural substances with higher efficiency and low toxic and side effects, so that products with good effect and high safety are provided for consumers.

Disclosure of Invention

The invention provides an anti-inebriation composition, which can be used for inebriation after drinking, can be drunk daily, has the effects of resisting oxidation, improving immunity and protecting liver, and has good safety and no toxic or side effect.

The invention provides a preparation method of an anti-inebriation composition, which can prepare the anti-inebriation composition with good safety and no toxic or side effect, and has simple preparation process and low preparation cost.

The invention provides an application of an anti-alcoholism composition, which can be used for preparing anti-alcoholism health-care products or anti-oxidation health-care products.

The health-care product provided by the invention can be used for relieving alcoholism after drinking, can be drunk daily, has the effects of resisting oxidation, improving immunity and protecting liver, and is good in safety and free of toxic and side effects.

The invention provides an anti-alcohol composition, which comprises the following components in percentage by mass:

20 to 60 percent of wild rice stem extract;

20-60% of blueberry extract;

10 to 30 percent of auxiliary material;

1 to 20 percent of nutritional supplement.

The hangover alleviating composition comprises the following components in percentage by mass:

25 to 45 percent of wild rice stem extract;

25-45% of blueberry extract;

12 to 20 percent of auxiliary material;

1 to 10 percent of nutritional supplement.

The hangover alleviating composition as described above, wherein the auxiliary material includes at least one of a pueraria extract, a honeysuckle extract, a medlar extract, a chrysanthemum extract, a tsaoko amomum fruit extract and a bamboo shavings extract.

The hangover alleviating composition comprises the following auxiliary materials in percentage by mass based on the total mass of the hangover alleviating composition:

2 to 10 percent of kudzu root extract;

1% -6% of honeysuckle extract;

1% -6% of medlar extract;

1% -6% of chrysanthemum extract;

1% -6% of amomum tsao-ko extract;

1 to 6 percent of bamboo shavings extract.

The anti-hangover composition as set forth above, wherein the nutritional supplement includes at least one of vitamin B, vitamin C, L-cysteine, L-theanine, and taurine.

The anti-hangover composition as set forth above, wherein the vitamin B includes at least one of vitamin B1, vitamin B2, and vitamin B6.

The invention provides a preparation method of the anti-alcoholism composition, wherein the anti-alcoholism composition is obtained by mixing cane shoot extract, blueberry extract, auxiliary materials and a nutritional supplement.

The invention provides an application of the hangover alleviating composition in a hangover alleviating health-care product or an antioxidant health-care product.

The invention provides a health-care product, wherein the health-care product comprises the anti-alcoholism composition.

The health product further comprises at least one of white granulated sugar, xylitol, honey, concentrated fruit juice, fruit powder, citric acid and malic acid.

The invention provides an anti-alcohol composition, which comprises a water bamboo extract, a blueberry extract, auxiliary materials and a nutritional supplement with specific contents.

The invention provides a preparation method of an anti-inebriation composition, the anti-inebriation composition with good safety and no toxic or side effect can be prepared by the preparation method, the preparation process is simple, and the preparation cost is low.

The invention provides an application of an anti-alcoholism composition, which can be used for preparing anti-alcoholism health-care products or anti-oxidation health-care products.

The invention provides a health-care product which comprises the anti-alcoholism composition, can be used for relieving alcoholism after drinking, can be eaten for a long time, has the effects of resisting oxidation, improving immunity and protecting liver, and has good safety and no toxic or side effect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the related art, the drawings used in the description of the embodiments of the present invention or the related art are briefly introduced below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

FIG. 1 is a histogram of the activation ability of Alcohol Dehydrogenase (ADH) in mice of each experimental group according to the present invention;

FIG. 2 is a histogram of the activation ability of acetaldehyde dehydrogenase (ALDH) in mice of each experimental group according to the present invention;

FIG. 3 is a histogram of the activation capacity of superoxide dismutase (SOD) in mice of each experimental group according to the present invention;

FIG. 4 is a histogram of the activation ability of Catalase (CAT) in mice of each experimental group of the present invention;

FIG. 5 is a histogram of total cholesterol (TG) in serum from mice in each experimental group according to the present invention;

FIG. 6 is a histogram of total Triglycerides (TC) in serum of mice in each experimental group of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides an anti-alcohol composition, which comprises the following components in percentage by mass:

20 to 60 percent of wild rice stem extract;

20-60% of blueberry extract;

10 to 30 percent of auxiliary material;

1 to 20 percent of nutritional supplement.

The wild rice stem is rich in nutrition, is rich in polysaccharide, protein, fiber, mineral substances, flavone, polyphenol and the like, and can be used for relieving alcoholism and daily health care. The cane shoot extract is obtained by the method comprising the following steps: cleaning water bamboo, putting the water bamboo into a container, adding purified water into the container, adjusting the pH value to 8.5, heating the water bamboo to 45-65 ℃, preserving the heat for 1-2 h, and then filtering to obtain a solution, wherein the solution is a water bamboo extract. The zizania latifolia extract can fully retain nutrient substances in the zizania latifolia, and improve the antialcoholic performance and the healthcare effect of the antialcoholic composition.

The blueberry contains rich nutrient and physiological active ingredients such as anthocyanin, flavone, polyphenol, calcium, phosphorus, potassium, vitamin C, vitamin E, vitamin B, dietary fiber and the like, and has the functions of resisting oxidation, resisting inflammation, protecting eyesight, resisting cancer, delaying aging, reducing blood fat and the like. The blueberry extract is obtained by extracting through the following method: mixing fructus Myrtilli and cellulase in an extraction tank for 10-50min, adding purified water, heating the extraction tank to 40-100 deg.C, extracting at constant temperature for 10-40min, and filtering to obtain a solution. In a specific embodiment, the mixing is carried out for 30min, and the extraction tank is heated to 60 deg.C for 20 min. The blueberry extract disclosed by the invention can more fully retain nutrient substances in blueberries, particularly the anthocyanin content in the blueberries, is favorable for prolonging the tolerance time of a human body to alcohol, shortening the sobering-up time and relieving the influence of alcohol on the activity of Alcohol Dehydrogenase (ADH).

The auxiliary materials are used for assisting and supplementing the anti-alcoholism composition and improving the anti-alcoholism effect of the anti-alcoholism composition, are not particularly limited, and can be food additive auxiliary materials commonly used in the field. The nutritional supplement is used for supplementing the nutritional ingredients of the anti-inebriation composition and improving the anti-inebriation effect and the health care function of the anti-inebriation composition, is not particularly limited, and can be selected from nutritional supplements commonly used in the field.

The anti-alcohol composition is obtained by matching the wild rice stem extract, the blueberry extract, the auxiliary materials and the nutritional supplement, so that the effects of the wild rice stem extract, the blueberry extract, the auxiliary materials and the nutritional supplement can be maximally exerted, and the obtained anti-alcohol composition not only has a good anti-alcohol effect, but also has a good daily health-care effect, such as oxidation resistance, immunity improvement, liver protection and the like. The components of the anti-inebriation composition are edible raw materials, and the combination of all the raw materials does not produce side effect, so the anti-inebriation composition has good safety and no toxic or side effect.

In some embodiments of the present invention, in order to obtain an anti-hangover composition with a better anti-hangover effect, the anti-hangover composition comprises the following components by mass:

25 to 45 percent of wild rice stem extract;

25-45% of blueberry extract;

12 to 20 percent of auxiliary material;

1 to 10 percent of nutritional supplement.

In some embodiments of the present invention, the adjuvant comprises at least one of puerariae radix extract, lonicera flos extract, lycii fructus extract, chrysanthemums extract, tsaoko amomum fruit extract and bambusae caulis extract.

The radix Puerariae is rich in functional substances such as flavonoids, triterpenoid saponins, alkaloids, etc., and has effects of regulating ethanol absorption and metabolism. The honeysuckle contains various trace elements and chemical components necessary for human bodies and has good antioxidant function. The chrysanthemum is bitter and sweet in taste and slightly cold in nature, can clear liver heat and calm liver yang, and has a protection effect on liver injury after drinking. The medlar is sweet and neutral in taste, has the functions of nourishing kidney, moistening lung and tonifying liver, is rich in anti-oxidation functional substances such as flavonoids, vitamin A, vitamin C, vitamin E and the like, and has a very strong anti-oxidation function. The tsaoko amomum fruit is pungent in taste, warm in nature and non-toxic, and can relieve vomiting symptom after drinking. The bamboo shavings are sweet in taste and slightly cold in nature, and can relieve headache symptom after drinking.

The extraction method of the kudzu root extract comprises the following steps: adding radix Puerariae into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 deg.C, extracting at constant temperature for 15-50min, and filtering to obtain a solution, which is radix Puerariae extract. In a specific embodiment, the extraction tank is heated to 80 ℃ and the extraction time at constant temperature is 30 min.

The extraction method of the honeysuckle extract comprises the following steps: adding flos Lonicerae into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 deg.C, extracting at constant temperature for 10-50min, and filtering to obtain a solution, which is flos Lonicerae extract. The honeysuckle extract obtained by the method can fully extract the nutrient components in the honeysuckle, and improve the antialcoholism effect and the oxidation resistance of the antialcoholism composition. In a specific embodiment, the extraction tank is heated to 100 ℃ and the extraction time at constant temperature is 30 min.

The extraction method of the medlar extract comprises the following steps: adding fructus Lycii into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 deg.C, extracting at constant temperature for 10-50min, and filtering to obtain a solution, which is fructus Lycii extract. The fructus Lycii extract obtained by the method can prevent loss of beneficial components in fructus Lycii as much as possible, and improve hangover alleviating effect and oxidation resistance of the hangover alleviating composition. In a specific embodiment, the extraction tank is heated to 100 ℃ and the extraction time at constant temperature is 30 min.

The extraction method of the chrysanthemum extract comprises the following steps: adding flos Chrysanthemi into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 deg.C, extracting at constant temperature for 10-50min, and filtering to obtain solution, which is flos Chrysanthemi extract. The chrysanthemum extract obtained by the method can fully retain the beneficial components of chrysanthemum and obtain the hangover-alleviating composition with good liver-protecting effect. In a specific embodiment, the extraction tank is heated to 100 ℃ and the extraction time at constant temperature is 30 min.

The extraction method of the tsaoko amomum fruit extract comprises the following steps: adding the tsaoko amomum fruits into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 ℃, carrying out constant-temperature extraction for 10-50min, and then filtering to obtain a solution, wherein the solution is the tsaoko amomum fruit extract. In a specific embodiment, the extraction tank is heated to 80 ℃ and the extraction time at constant temperature is 30 min.

The extraction method of the bamboo shavings extract comprises the following steps: adding the bamboo shavings into an extraction tank, adding purified water into the extraction tank, heating the extraction tank to 60-100 ℃, extracting at constant temperature for 10-50min, and filtering to obtain a solution, wherein the solution is the bamboo shavings extract. In a specific embodiment, the extraction tank is heated to 80 ℃ and the extraction time at constant temperature is 30 min.

The auxiliary materials of the invention can further improve the antialcoholism effect and the antioxidation effect of the antialcoholism composition.

In some embodiments of the present invention, the anti-hangover effect and the antioxidant effect of the anti-hangover composition are further enhanced. Based on the total mass of the anti-alcoholism composition, the auxiliary materials comprise the following components in percentage by mass:

2 to 10 percent of kudzu root extract;

1% -6% of honeysuckle extract;

1% -6% of medlar extract;

1% -6% of chrysanthemum extract;

1% -6% of amomum tsao-ko extract;

1 to 6 percent of bamboo shavings extract.

In some embodiments of the invention, the nutritional supplement comprises at least one of vitamin B, vitamin C, L-cysteine, L-theanine, and taurine.

Further, the vitamin B includes at least one of vitamin B1, vitamin B2, and vitamin B6.

The second aspect of the invention provides a preparation method of the hangover alleviating composition, which is to mix cane shoot extract, blueberry extract, auxiliary materials and nutritional supplements to obtain the hangover alleviating composition.

The invention does not limit the mixing mode of the wild rice stem extract, the blueberry extract, the auxiliary materials and the nutritional supplement, and the anti-alcohol composition can be obtained by mixing the wild rice stem extract, the blueberry extract and the auxiliary materials and then mixing the mixture with the nutritional supplement; or mixing caulis Zizaniae Caduciflorae extract, fructus Myrtilli extract, adjuvants and nutritional supplement directly to obtain the composition for relieving hangover. An appropriate amount of water may also be added during the mixing process.

The preparation method of the anti-inebriation composition can prepare the anti-inebriation composition with good anti-inebriation property and oxidation resistance, and the anti-inebriation composition has the advantages of good safety, no toxic or side effect, simple preparation process and low preparation cost.

The third aspect of the invention provides an application of the hangover alleviating composition in a hangover alleviating health care product or an antioxidant health care product. The anti-hangover composition can be used for preparing anti-hangover health products and antioxidant health products.

The fourth aspect of the invention provides a health care product, which comprises the anti-alcohol composition.

According to the invention, the health-care product comprises the anti-alcoholism composition, so that the anti-alcoholism can be realized after drinking, the health-care product can be eaten for a long time, has the effects of resisting oxidation, improving immunity and protecting liver, and is good in safety and free of toxic and side effects.

In some embodiments of the present invention, the health product may be prepared as a liquid beverage, a solid beverage or a gel soft candy by adding at least one of white granulated sugar, xylitol, honey, concentrated fruit juice, fruit powder, citric acid, and malic acid to the health product.

In some embodiments of the present invention, the type of nutraceutical may also include powders, tablets, granules, pills or capsules.

The following explains the technical aspects of the present invention with reference to specific embodiments.

Example 1

The anti-alcohol composition of the embodiment is prepared by the following method:

putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture; mixing the mixture with vitamin B6, vitamin C, L-cysteine, taurine and theanine in a mixer to obtain the hangover alleviating composition;

as shown in table 1, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 1

Example 2

The anti-alcohol composition of the embodiment is prepared by the following method:

putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture;

adding vitamin B1, vitamin B2, vitamin B6, vitamin C, L-cysteine, taurine and theanine to the mixture;

as shown in table 2, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 2

Example 3

The anti-alcohol composition of the embodiment is prepared by the following method:

putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture;

adding vitamin B1, vitamin B2, vitamin B6, vitamin C, L-cysteine, taurine and theanine, vitamin C, L-cysteine and taurine into the mixture to obtain the anti-hangover composition;

as shown in table 3, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 3

Example 4

Putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture;

adding vitamin B1, vitamin B2, vitamin B6, vitamin C, L-cysteine, taurine and theanine to the mixture;

as shown in table 4, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 4

Example 5

Putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture;

adding vitamin B1, vitamin B2, vitamin B6, vitamin C, L-cysteine, taurine and theanine to the mixture;

as shown in table 5, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 5

Example 6

Putting wild rice stem extract, blueberry extract, kudzu root extract, honeysuckle extract, medlar extract, chrysanthemum extract, tsaoko amomum fruit extract and bamboo shavings extract into a mixer, and uniformly mixing to obtain a mixture;

adding vitamin B1, vitamin B2, vitamin B6, vitamin C, L-cysteine, taurine and theanine to the mixture;

as shown in table 6, the anti-hangover composition comprises the following components in percentage by mass:

TABLE 6

Comparative example 1

The preparation method of the anti-hangover composition of this comparative example is substantially the same as that of example 2, except that the zizania latifolia extract of example 2 is replaced with water.

Comparative example 2

The preparation method of the anti-alcoholism composition of the comparative example is basically the same as that of the example 2, and the only difference is that the blueberry extract in the example 2 is replaced by water.

Comparative example 3

The preparation method of the anti-inebriation composition of the comparative example is basically the same as that of the example 2, and the only difference is that the auxiliary materials in the example 2 are replaced by water.

Comparative example 4

The preparation method of the anti-hangover composition of this comparative example is substantially the same as that of example 2, except that the nutrition enhancer of example 2 is replaced with water.

Comparative example 5

The preparation method of the anti-hangover composition of the comparative example is basically the same as that of example 2, except that the mixture ratio in example 2 is as shown in table 7:

TABLE 7

Comparative example 6

The preparation method of the anti-hangover composition of the comparative example is basically the same as that of example 2, except that the wild rice shoot extract in example 2 is replaced by a blueberry extract.

Comparative example 7

The preparation method of the anti-alcoholism composition of the comparative example is basically the same as that of the example 2, and the only difference is that the auxiliary materials in the example 2 are replaced by the blueberry extract.

Test examples

1. The anti-hangover compositions obtained in the examples and the respective proportions and the anti-hangover agents (hereinafter referred to as anti-hangover agents 1) mainly containing oyster fine powders in the market were subjected to the following tests on anti-hangover composition solutions, the test results are shown in table 8, and the test methods are as follows:

(1) determination of radical scavenging ability of 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH)

Sample group: adding 1mL of the hangover alleviating composition solution of example 1 to 1mL of a 0.2mmol/L DPPH-absolute ethanol solution;

control group: adding 1mL of the anti-hangover composition solution of example 1 to 1mL of Phosphate Buffered Saline (PBS);

blank group: adding 1mL of PBS into 1mL of DPPH-absolute ethanol solution with the concentration of 0.2 mmol/L;

reacting the solutions obtained from the sample group, the control group and the blank group at room temperature in a dark place for 30 min; centrifuging at 3500r/min for 10min, measuring the absorbance of the supernatant at 517nm, and calculating the DPPH free radical clearance rate according to the following formula:

wherein: a. the_iIs the absorbance of the sample set;

A_jabsorbance of control group;

A_cabsorbance for blank group;

the DPPH radical scavenging ability of the other examples, the anti-hangover composition obtained in each of the comparative examples, and the commercial anti-hangover drug 1 was measured in the same manner as that of the anti-hangover composition of example 1.

(2) Determination of Hydroxyl Radical (HR) scavenging Capacity

Sample group: to 1mL of a PBS (pH 7.4) solution with a concentration of 0.02mol/L were added 0.5mL of a 2.5mmol/L phenanthroline solution, and 0.5mL of a 2.5mmol/L FeSO solution₄Solution and 0.5mL of 20mmol/L H₂O₂Mixing the solution, and adding 0.5mL of the hangover alleviating composition solution of example 1;

control group: to 1mL of a PBS (pH 7.4) solution with a concentration of 0.02mol/L were added 0.5mL of a 2.5mmol/L phenanthroline solution, and 0.5mL of a 2.5mmol/L FeSO solution₄Solution and 0.5mL of 20mmol/L H₂O₂Fully and uniformly mixing the solution, and then adding 0.5mL of distilled water;

blank group: to 1mL of a PBS (pH 7.4) solution with a concentration of 0.02mol/L were added 0.5mL of a 2.5mmol/L phenanthroline solution, and 0.5mL of a 2.5mmol/L FeSO solution₄Solution and 1mL of distilled water;

the sample group, the control group and the blank group were placed in a thermostatic water bath at 37 ℃ for 1h, and then the absorbance was measured at 536nm, and the hydroxyl radical clearance was calculated according to the following formula:

clearance of hydroxyl radical (· OH)/% (As-Ab)/(Ac-Ab) × 100%;

wherein: as is the absorbance of the sample set;

ac is the absorbance of the control group;

ab is absorbance of blank;

the measurement of the hydroxyl radical scavenging ability of the other examples, the anti-hangover composition obtained in each comparative example, and the commercial anti-hangover drug 1 was the same as the measurement of the hydroxyl radical scavenging ability of the anti-hangover composition in example 1.

(3)2,2' -biazo-bis-3-ethylbenzothiazoline-6-sulfonic Acid (ABTS) scavenging ability assay

Sample group: adding 0.1mL of the hangover alleviating composition solution of example 1 into 1mL of ABTS reaction solution, mixing uniformly, and reacting at room temperature for 30 min;

control group: adding 0.1mL of distilled water into 1mL of ABTS reaction solution, mixing uniformly, and reacting at room temperature for 30 min;

blank group: 1.1mL of distilled water;

the absorbance at 734nm of the sample, control and blank groups was measured and the ABTS free radical clearance was calculated according to the following formula:

wherein: as is the absorbance of the sample set;

ac is the absorbance of the control group;

ab is absorbance of blank;

the ABTS removing ability of other examples, the anti-inebriation composition obtained in each comparative example, and the commercial anti-inebriation drug 1 was measured in the same manner as the ABTS removing ability of the anti-inebriation composition of example 2.

(4) Alcohol Dehydrogenase (ADH) activation ability assay

Sample group: to a test tube were added 1.5mL of sodium pyrophosphate buffer (pH 8.8) and 1.0mL of 0.027mol · L in that order^-1Is oxidized nicotinamide adenine dinucleotide (NAD +), 0.5mL of 12% ethanol by volume, and 0.1mL of the composition of example 1Mixing the above alcoholic intoxication relieving composition solution, sealing and incubating at 25 deg.C for 5min, immediately adding 0.1mL 2.5 enzyme activity unit/mL ADH into test tube, shaking, and immediately measuring absorbance A at 340nm with enzyme labeling instrument₃₄₀Reading the absorbance value every 10s for 1 time at nm, continuously measuring for 10min, and recording data;

blank group: replacing 0.1mL of the anti-hangover composition solution of example 1 in the sample group with 0.1mL of sodium pyrophosphate buffer, and leaving the steps unchanged;

the activation rate (a) of ADH is calculated according to the following formula:

A＝E₁/E₀×100％；

E₁＝(A₀₁×3.2)/(2.5×6.2)；

E₀＝(A₀₀×3.2)/(2.5×6.2)；

wherein: a is the activation rate of ADH, E₁For the sample histone enzyme activity, E₀Is blank group enzyme activity; fitting curves to the 340nm wavelength variation recorded for 10min reaction for the blank and sample groups, A₀₀The average increment of the absorbance in each 1min in the blank group fitting curve is obtained, namely the variation of the absorbance value of the fitting curve in 10min is divided by the time in 10 min; a. the₀₁The average increment of the absorbance in each 1min in the fitted curve of the sample group is obtained, namely the variation of the absorbance value of the fitted curve in 10min is divided by the time in 10 min; 2.5 is the enzyme content in each group of enzyme solution; 3.2 is the total volume of the reaction solution, 6.2 is the molar extinction coefficient of reductive Nicotinamide Adenine Dinucleotide (NADH) at 340 nm;

the other examples, the anti-hangover compositions obtained in respective proportions, and the ADH activation ability of the anti-hangover drug 1 on the market were measured in the same manner as the ADH activation ability of the anti-hangover composition in example 1.

TABLE 8

As can be seen from table 8, the DPPH radical scavenging rate of the anti-hangover composition prepared in the examples is greater than that of the comparative example and the anti-hangover drug 1, and the anti-hangover composition of the present invention is proved to have strong DPPH radical scavenging ability and good oxidation resistance.

The hydroxyl radical clearance rate of the anti-inebriation composition prepared in the embodiment is greater than that of the comparative example and the anti-inebriation medicine 1, and the anti-inebriation composition is proved to have strong hydroxyl radical clearance capability and good oxidation resistance.

The ABTS clearance of the anti-inebriation composition prepared in the embodiment is larger than that of the comparative example and the anti-inebriation drug 1, and the anti-inebriation composition is proved to have strong ABTS clearance, good oxidation resistance and capability of improving the immunity.

The ADH activation rates of the anti-inebriation composition prepared in the examples are all larger than that of the ADH activation rate of the comparative example and the anti-inebriation drug 1, and the anti-inebriation composition is proved to have strong ADH activation capacity and good anti-inebriation and liver protection effects.

2. Animal experiments were carried out on the anti-hangover composition prepared in example 4

1) Sample preparation

The anti-hangover composition obtained in example 2 was prepared into anti-hangover composition solutions having concentrations of 20mg/mL, 10mg/mL, and 5mg/mL, respectively.

2) Packet processing

After six-week-old male Balb/C mice were acclimatized for one week, the initial ADH, initial ALDH, initial SOD and initial CAT contents in each mouse were tested separately, and then the mice were randomly grouped into groups of 10 mice per group as follows:

blank group (NC group): balb/c mice were gazed with 1mL/100g of 0.85% physiological saline daily;

control group (AM group): balb/c mice were gazed with 1mL/100g of 0.85% physiological saline daily;

HNE group: the Balb/c mice are intragastrically filled with 1mL/100g of the anti-inebriation composition solution of 200mg/kg of the mouse body weight every day;

MNE group: the Balb/c mice are intragastrically filled with 1mL/100g of 100mg/kg of the mouse weight of the anti-inebriation composition solution every day;

LNE group: the Balb/c mice are intragastrically filled with 50mg/kg of the anti-inebriation composition solution of 1mL/100g of the mouse weight per day;

after each group was continuously gazed for 7 days, mice in the AM group, HNE group, MNE group and LNE group were separately gazed with 0.2mL of 52 ° distilled spirit, and an alcoholism model was established.

3) Test results

A. The final ADH content in each mouse was tested and the ADH activating ability of each mouse was calculated by the following formula:

ADH activating ability of mice ═ (final ADH content-initial ADH content)/initial ADH content 100%;

according to the grouping situation, the average value of the ADH activation capability of each group of mice is calculated, and a histogram is drawn. FIG. 1 is a histogram of the activation ability of Alcohol Dehydrogenase (ADH) in mice in each experimental group of the present invention. As can be seen from fig. 1, the ADH activation ability of the mice in the LNE, MNE and HNE groups is greater than the ADH activation ability of the mice in the NC and AM groups, which indicates that the anti-hangover composition of the present invention can improve the ADH activation ability of the mice, improve the anti-hangover ability and liver protection ability; and the ADH activation capability of the mice in the LNE, MNE and HNE groups is gradually increased, which shows that the high-concentration anti-inebriation composition solution can obviously improve the ADH activation capability of the mice, and improve the anti-inebriation capability and the liver protection capability.

B. The calculation of the ALDH, SOD and CAT activating ability of each group of mice was the same as the calculation of the ADH activating ability of each group of mice.

Fig. 2 is a histogram of the activation capacity of acetaldehyde dehydrogenase (ALDH) of mice in each experimental group of the present invention, and it can be seen from fig. 2 that the ALDH activation capacity of the mice in the LNE, MNE and HNE groups is greater than the ALDH activation capacity of the mice in the NC and AM groups, which indicates that the anti-hangover composition of the present invention can improve the ALDH activation capacity of the mice, enhance the anti-hangover capacity and liver protection capacity; the ALDH activation capability of mice in the LNE, MNE and HNE groups is gradually increased, which shows that the high-concentration anti-inebriation composition solution can obviously improve the ALDH activation capability of the mice, and improve the anti-inebriation capability and the liver protection capability.

FIG. 3 is a histogram of the activation capacity of superoxide dismutase (SOD) in mice of each experimental group of the present invention, and it can be seen from FIG. 3 that the SOD activation capacity of mice in the AM, LNE, MNE and HNE groups is smaller than that of mice in the NC group, because the SOD activation capacity in the mouse body is decreased after the mice are drunk; the SOD activation capacity of mice in the LNE, MNE and HNE groups is larger than that of mice in the AM group, which shows that the hangover alleviating composition can improve the SOD activation capacity of the mice, the hangover alleviating capacity and the liver protecting capacity; the SOD activation capability of mice in LNE, MNE and HNE groups is gradually increased, which shows that the high-concentration sobering-up composition solution can obviously improve the SOD activation capability of the mice, and improve the sobering-up capability and the liver protection capability.

FIG. 4 is a histogram of the activation ability of Catalase (CAT) in mice of each experimental group of the present invention, as shown in FIG. 4, the CAT activation ability of the mice in the AM, LNE, MNE and HNE groups is smaller than that of the mice in the NC group, because the CAT activation ability in the mice decreases after the mice are drunk; the CAT activation capability of mice in LNE, MNE and HNE groups is greater than that of mice in AM group, which shows that the anti-alcoholism composition can improve the CAT activation capability of mice, the anti-alcoholism capability and the liver protection capability; the CAT activation capability of mice in LNE, MNE and HNE groups is gradually increased, which shows that the anti-inebriation composition solution with high concentration can obviously improve the CAT activation capability of the mice, and improve the anti-inebriation capability and the liver protection capability.

C. Total Triglyceride (TG) levels in the serum of each mouse were tested, and the mean TG levels in the serum of each group of mice were calculated and plotted in a histogram. FIG. 5 is a histogram of TG in serum of mice in each experimental group of the present invention, and as shown in FIG. 5, TG content in serum of mice in AM, LNE, MNE and HNE groups is greater than TG content in serum of mice in NC group, because TG content in serum of mice increases after the mice are drunk; the TG content in the serum of the mice in the LNE, MNE and HNE groups is less than that in the serum of the mice in the AM group, so that the antialcoholic composition can reduce the TG content in the serum of the mice and has good antialcoholic capability; and the TG content in the serum of the mice in the LNE, MNE and HNE groups is gradually reduced, which shows that the antialcoholic composition solution with high concentration can obviously reduce the TG content in the serum of the mice and has better antialcoholic effect.

Total Cholesterol (TC) levels in the serum of each mouse were tested, and the mean value of TC levels in the serum of each group of mice was calculated and plotted as a histogram. FIG. 6 is a histogram of TC in serum of mice in each experimental group of the present invention, as shown in FIG. 6, the TC content in serum of mice in AM, LNE, MNE and HNE groups is greater than the TC content in serum of mice in NC group, because the TC content in serum of mice increases after the mice are drunk; the TC content in the serum of the mice in the LNE, MNE and HNE groups is less than that in the serum of the mice in the AM group, so that the antialcoholic composition can reduce the TG content in the serum of the mice and has good antialcoholic capacity; and the TG content in the serum of the mice in the LNE, MNE and HNE groups is gradually reduced, which shows that the antialcoholic composition solution with high concentration can obviously reduce the TG content in the serum of the mice and has better antialcoholic effect.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.