具体实施方式

本申请涉及纳米颗粒和基于纳米颗粒的疫苗，其引发针对来自广泛流感病毒的血凝素(HA)蛋白的免疫应答。这些疫苗含有自组装纳米颗粒，在其表面表达流感病毒HA蛋白的免疫原性部分。纳米颗粒包括两个独特的组件，它们非共价相互作用形成纳米颗粒。这些组件包括已经被设计成自组装成纳米颗粒的合成蛋白质，其中合成蛋白质(构建块)根据特定的数学对称群三维排列。在这些纳米颗粒中，至少一些组件包含与流感病毒HA蛋白的免疫原性部分连接的自组装蛋白，使得HA蛋白的免疫原性部分在纳米颗粒的表面表达。这种纳米颗粒可用于例如为受试者接种流感病毒疫苗，以及检测抗流感抗体或流感特异性B细胞。本申请还涉及用于制备所述纳米颗粒的蛋白质，包括融合蛋白，以及编码所述蛋白质的核酸分子。此外，本申请涉及制备本申请的纳米颗粒的方法，以及使用制备纳米颗粒为受试者接种疫苗的方法。

本文用的术语仅用于描述特定的实施方案，而非旨在限制，因为本申请的范围将仅由权利要求来限制。

如本文和所附权利要求中所使用的，单数形式“一(a)”、“一个(an)”和“所述(the)”包括复数指代物，除非上下文另有明确规定。例如，核酸分子是指一个或多个核酸分子。因此，术语“一”、“一个”、“一个或多个”和“至少一个”可以互换使用。类似地，术语“包含(comprising)”、“包括(including)”和“具有(having)”可以互换使用。还应注意，权利要求可以被起草为排除任何可选元素。因此，本陈述旨在作为与权利要求元素的叙述或“否定”限制的使用相关的诸如“只”、“仅”等专有术语使用的先行基础。

除非另有定义，本文使用的所有技术和科学术语与本发明所属领域的普通技术人员通常理解的含义相同。尽管在本申请的实践或测试中也可以使用类似于或等同于本文描述的任何方法和材料，但是本文描述优选的方法和材料。

本申请的纳米颗粒包括：1)由多个相同的第一蛋白质组成的多个第一组件；和2)由多个相同的第二蛋白质组成的多个第二组件，其中至少一个第二组件包含与流感血凝素(HA)蛋白质的免疫原性部分连接的第二蛋白质，从而形成融合蛋白。在这些纳米颗粒中，第一蛋白质的序列可以不同于第二蛋白质的序列。此外，多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒，并且由多个第一和第二组件组装的纳米颗粒在其表面表达HA蛋白的免疫原性部分。

如本文所用，纳米颗粒是指由本申请的第一和第二组件的非共价相互作用形成的三维结构。本申请的纳米颗粒可以具有适于预期用途的任何形状和/或对称性，包括但不限于四面体、八面体、二十面体、十二面体及其截断形式。本申请的纳米颗粒可以是任何适于预期用途的尺寸，包括但不限于约10nm至约100nm的直径。

本申请的将第一和第二组件组装成纳米颗粒不是随机的，而是由非共价相互作用(例如氢键、静电、范德华力、疏水性等)决定的(即，第一组件之间的相互作用、第二组件之间的相互作用以及第一组件和第二组件之间相互作用的累积效应)。因此，本申请的纳米颗粒包括对称重复的、非天然的、非共价的蛋白质-蛋白质界面，所述界面将第一和第二组件定向组装成具有高度有序结构的纳米颗粒。虽然纳米颗粒的形成是由于第一和第二组件的非共价相互作用，但是在一些实施方案中，纳米颗粒一旦形成，即可以通过第一组件和第二组件中的蛋白质之间的共价连接而变得稳定。可以使用任何适宜的共价键，包括但不限于二硫键和异肽键。

如本文所用，组件是由本申请的多个(2个、3个、4个、5个、6个或更多个)自组装蛋白质(即，第一和第二蛋白质)的非共价相互作用产生的多聚体结构。因此，本申请的组件可以包括但不限于二聚体、三聚体、四聚体、五聚体、六聚体、七聚体、八聚体、九聚体、十聚体等。单个自组装蛋白质的非共价相互作用使得第一蛋白质自组装成第一组件，第二蛋白质自组装成第二组件。第一组件中第一蛋白质的数量可以与第二组件中第二蛋白质的数量相同或不同。因此，例如，本申请的纳米颗粒可以包括五聚体的第一组件和三聚体的第二组件。虽然组件的形成是由于多种自组装蛋白质的非共价相互作用，但是在一些实施方案中，组件一旦形成，则可以通过组件内蛋白质的共价连接而变得稳定。因此，例如，可以通过共价交联第一组件中的第一蛋白质来稳定第一组件。

适用于制备本申请的组件的第一蛋白质和第二蛋白质对于给定的纳米颗粒可以是任何合适的长度。第一蛋白质和第二蛋白质的长度可以在30～250个氨基酸之间、30～225个氨基酸之间、30～200个氨基酸之间、30～175个氨基酸之间、50～250个氨基酸之间、50～225个氨基酸之间、50～200个氨基酸之间、50～175个氨基酸之间、75～250个氨基酸之间、75～225个氨基酸之间、75～200个氨基酸之间、75～175个氨基酸之间、100～250个氨基酸之间、100～225个氨基酸之间、100～200个氨基酸之间、100～175个氨基酸之间、125～250个氨基酸之间、125～225个氨基酸之间、125～200个氨基酸之间、125～175个氨基酸之间、150～250个氨基酸之间、150～250个氨基酸之间、150～225个氨基酸之间、150～200个氨基酸之间和150～175个氨基酸之间。

如上所述，本申请的第一蛋白质自组装形成本申请的第一组件。同样，本申请的第二蛋白质自组装形成第二组件。自组装是指分子(如蛋白质)与多个相同分子相互作用形成有序多聚体结构(如二聚体、三聚体、四聚体、五聚体等)的能力。因此，如本文所用，自组装蛋白质是当在适当条件下孵育时，与多个相同的自组装蛋白质非共价相互作用以形成有序多聚体结构(例如，第一组件或第二组件)的蛋白质。在最终的多聚体组件中，每个自组装蛋白质(例如，第一蛋白质、第二蛋白质)的氨基酸序列是相同的。因此，本申请的第一组件包含第一蛋白质，其序列相同。同样地，本申请的第二组件包含第二蛋白质，其序列是相同的。第一和第二蛋白质的序列可以彼此不同。

本申请的自组装蛋白质是合成的，这意味着它们不是天然存在的。如本文所用，诸如合成的、非天然存在的、非天然的等短语意味着未在自然界中发现。因此，本申请的第一和第二蛋白质的序列未在自然界中发现。因此，此类蛋白质和多肽由人工设计，例如使用计算机蛋白质设计和重组DNA技术设计得到。本申请的自组装蛋白质已经被设计成使得多个相同的自组装蛋白质相互作用以自组装成多聚体组件。对于本申请的第一或第二蛋白质的一级氨基酸序列没有特定的序列要求，除了所述序列必须能够实现蛋白质自组装成多聚体组件，例如本申请的第一或第二组件。这种蛋白质的实例在本文和专利号为No.9,630,994、PCT公开号WO 2014/124301及PCT公开号WO 2018/187325的美国专利中公开，其全部内容通过引用并入本文。

在各种非限制性实施方案中，纳米颗粒的每个第一蛋白质包含或由与下表1中列出的第一蛋白质的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成：

表1示例性核酸和氨基酸序列

在非限制性实施方案中，这些第一蛋白质可以包含或由与选自SEQ ID NO:41、SEQID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQ ID NO:65、SEQID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:227、SEQ ID NO:229、SEQ ID NO:231、SEQ ID NO:233、SEQ ID NO:235、SEQ ID NO:237、SEQ IDNO:239、SEQ ID NO:241、SEQ ID NO:243、SEQ ID NO:267、SEQ ID NO:268、SEQ ID NO:271、SEQ ID NO:273、SEQ ID NO:275、SEQ ID NO:276、SEQ ID NO:279、SEQ ID NO:280、SEQ IDNO:281、SEQ ID NO:282、SEQ ID NO:285、SEQ ID NO:287、SEQ ID NO:288、SEQ ID NO:291、SEQ ID NO:293，SEQ ID NO:295、SEQ ID NO:297、SEQ ID NO:299、SEQ ID NO:347、SEQ IDNO:348和SEQ ID NO：349的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成，其中多个第一蛋白质相互作用形成第一组件。在进一步的实施方案中，这些第一蛋白质可以包含或由与选自SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:227、SEQID NO:229SEQ ID NO:231、SEQ ID NO:233、SEQ ID NO:235、SEQ ID NO:237、SEQ ID NO:239、SEQ ID NO:241、SEQ ID NO:243、SEQ ID NO:267、SEQ ID NO:268、SEQ ID NO:271、SEQID NO:273、SEQ ID NO:275、SEQ ID NO:282、SEQ ID NO:285、SEQ ID NO:287、SEQ ID NO:288、SEQ ID NO:291、SEQ ID NO:293、SEQ ID NO:295、SEQ ID NO:297、SEQ ID NO:299、SEQID NO:347、SEQ ID NO:348和SEQ ID NO:349的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个第一蛋白质相互作用形成第一组件。

在这些纳米颗粒中，多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒，并且纳米颗粒在纳米颗粒的外表面表达流感病毒HA蛋白的免疫原性部分。在这些纳米颗粒中，第二组件中的每个第二蛋白质可以包含、含有或由与表1中列出的第二蛋白质的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成。在非限制性实施方案中，这些第二蛋白质可以包含或由与选自SEQ ID NO:89、SEQID NO:245、SEQ ID NO:247、SEQ ID NO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255、SEQ ID NO:257、SEQ ID NO:266、SEQ ID NO:269、SEQ ID NO:270、SEQ ID NO:272、SEQID NO:274、SEQ ID NO:277、SEQ ID NO:278、SEQ ID NO:281、SEQ ID NO:283、SEQ ID NO:284、SEQ ID NO:286、SEQ ID NO:289、SEQ ID NO:290、SEQ ID NO:292、SEQ ID NO:294、SEQID NO:296和SEQ ID NO:298的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成，其中多个第二蛋白质相互作用以形成第二组件。在其他实施方案中，这些第二蛋白质可以包含或由与选自SEQ ID NO:89、SEQ ID NO:245、SEQ ID NO:247、SEQ ID NO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255、SEQID NO:257、SEQ ID NO:266、SEQ ID NO:269、SEQ ID NO:270、SEQ ID NO:272、SEQ ID NO:274、SEQ ID NO:283、SEQ ID NO:284、SEQ ID NO:286、SEQ ID NO:289、SEQ ID NO:290、SEQID NO:292、SEQ ID NO:294、SEQ ID NO:296和SEQ ID NO:298的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成，其中多个第二蛋白质相互作用以形成第二组件。

在一个非限制性实施方案中，纳米颗粒的每种第二蛋白质包含或由与选自SEQ IDNO:89、SEQ ID NO:245、SEQ ID NO:247、SEQ ID NO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255、SEQ ID NO:257、SEQ ID NO:266、SEQ ID NO:269、SEQ ID NO:270、SEQ IDNO:272、SEQ ID NO:274、SEQ ID NO:277、SEQ ID NO:278、SEQ ID NO:281、SEQ ID NO:283、SEQ ID NO:284、SEQ ID NO:286、SEQ ID NO:289、SEQ ID NO:290、SEQ ID NO:292、SEQ IDNO:294、SEQ ID NO:296、SEQ ID NO:298的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个蛋白质非共价相互作用形成第二个组件。在进一步的实施方案中，纳米颗粒的每种第二蛋白质包含或由与选自SEQID NO:89、SEQ ID NO:245、SEQ ID NO:247、SEQ ID NO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255、SEQ ID NO:257、SEQ ID NO:266、SEQ ID NO:269、SEQ ID NO:270、SEQID NO:272、SEQ ID NO:274、SEQ ID NO:283、SEQ ID NO:284、SEQ ID NO:286、SEQ ID NO:289、SEQ ID NO:290、SEQ ID NO:292、SEQ ID NO:294、SEQ ID NO:296、SEQ ID NO:298的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个蛋白质非共价相互作用形成第二个组件。在这些纳米颗粒中，多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒，并且纳米颗粒在纳米颗粒的外部表达流感病毒HA蛋白的免疫原性部分。

在本申请的纳米颗粒的非限制性实施例中，每种第一蛋白质包含或由与选自SEQID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQID NO:347、SEQ ID NO:348的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第一蛋白质非共价相互作用形成第一组件。在这些纳米颗粒的非限制性实施例中，每个第二蛋白质包含或由与SEQ IDNO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第二蛋白质非共价相互作用形成第二组件。在另一个实施方案中，在本申请的纳米颗粒中，每种第一蛋白质包含或由与选自SEQ ID NO:43、SEQID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQ ID NO:65、SEQ ID NO:67、SEQID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:347、SEQ ID NO:348和SEQ ID NO:349的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第一蛋白质非共价相互作用形成第一组件。在这些纳米颗粒的非限制性实施例中，每个第二蛋白质包含或由与SEQ ID NO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第二蛋白质非共价相互作用形成第二组件。

在本申请的所有实施方案的纳米颗粒中，多个第一组件与多个第二组件非共价相互作用形成纳米颗粒，并且纳米颗粒在纳米颗粒的外部表达流感病毒HA蛋白的免疫原性部分。含HA的纳米颗粒的制备可以通过体外组装已纯化的蛋白质组分来完成，这可以模块化地适应同一颗粒上不同数量和种类的HA。

在这些纳米颗粒的进一步的非限制性实施例中，每个第一蛋白质包含或由与选自SEQ ID NO:227、SEQ ID NO:229SEQ ID NO:231、SEQ ID NO:233、SEQ ID NO:235、SEQ IDNO:237、SEQ ID NO:239、SEQ ID NO:241、SEQ ID NO:243的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第一蛋白质非共价相互作用形成第一组件。在这些纳米颗粒的其它非限制性实施例中，每种第二蛋白质包含或由与选自SEQ ID NO:245、SEQ ID NO:247、SEQ ID NO:249、SEQ IDNO:251、SEQ ID NO:253、SEQ ID NO:255、SEQ ID NO:257的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第二蛋白质非共价相互作用形成第二组件。

在这些纳米颗粒中，多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒，并且纳米颗粒在纳米颗粒的外部表达流感病毒HA蛋白的免疫原性部分的多个拷贝。含HA的纳米颗粒的制备可以通过体外组装已纯化的蛋白质组分来完成，这可以模块化地适应同一颗粒上不同数量和种类的HA。

在本申请的纳米颗粒的非限制性实施例中，第一蛋白质和第二蛋白质分别包含或分别由与选自表2中第一和第二蛋白质对的第一和第二蛋白质的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成：

表2

表3提供了示例性的第一和第二蛋白质的氨基酸序列；表3中的右栏标出了每个示例性蛋白质中被识别为存在于所得的组装纳米结构界面的残基(即:“已识别的界面残基”)。可以看出，示例性第一和第二蛋白质的界面残基的数量范围为4～21。在各种实施方案中，第一和第二蛋白质包含与表3中列出的第一和/或第二蛋白质至少有80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％相同的氨基酸序列，且与表3中列出的第一和/或第二蛋白质至少在1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20或21个相同的识别界面位置(取决于给定蛋白质的界面残基的数量)有相同的氨基酸序列。该实施方案可以与本文的所有其他实施方案相结合。

与一般蛋白质的情况一样，预期蛋白质能允许所设计的序列中存在变种，而不破坏随后组装成的纳米结构：特别是当这些变异包含保守氨基酸的取代时。本文所说的“保守氨基酸的取代”是指：疏水性氨基酸(Ala、Cys、Gly、Pro、Met、See、Sme、Val、Ile、Leu)只能被其他疏水性氨基酸取代；具有庞大侧链的疏水性氨基酸(Phe、Tyr、Trp)只能被其他具有庞大侧链的疏水性氨基酸取代；带正电荷侧链的氨基酸(Arg、His、Lys)只能被其他带正电荷侧链的氨基酸取代；带负电荷侧链的氨基酸(Asp，Glu)只能被其他带负电荷侧链的氨基酸取代；而具有极性不带电荷侧链的氨基酸(Ser、Thr、Asn、Gln)只能被其他具有极性不带电荷侧链的氨基酸取代。

表3

表4列出了本申请示例性的第一和第二蛋白质的表面氨基酸残基数。因此，在各种实施方案中，这些表达残基中的1个或更多(至少1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50或更多)可以在表4中所列出的第一和/或第二蛋白质中被修饰。该实施方案可以与本文的所有其他实施方案相结合。括号中的残基是可选的。

表4

为了制备本申请的自组装纳米颗粒，第一和第二蛋白质可以独立地表达和纯化。在这个非限制性实施例中，如图1A所示，第一和第二蛋白质在体外的混合启动了含HA的纳米颗粒的组装过程。或者，第一和第二蛋白质，包括与HA蛋白的免疫原性部分结合的第二蛋白质，可以在同一细胞中共表达。在这样的系统中，第一和第二蛋白质的共表达将使得纳米颗粒在细胞内和/或细胞纯化期间形成。

如上所述，本申请的纳米颗粒包含至少一个第二组件，所述第二组件包含与流感病毒HA蛋白的免疫原性部分连接的第二蛋白质。这种蛋白质可以称为融合蛋白。如本文所用，融合蛋白是一种蛋白质，其中两个或多个氨基酸序列共价结合在一起以产生新的、连续的氨基酸序列，该氨基酸序列不是天然存在的，因此是人工制备的。应当理解，由于本申请的融合蛋白包含本申请的第二蛋白质的序列，所以融合蛋白可以自组装，从而形成本申请的第二组件，这种第二组件包含HA蛋白的免疫原性部分。因此，包含与HA蛋白的免疫原性部分连接的第二蛋白质的蛋白质是融合蛋白的一个实例。HA蛋白的免疫原性部分可以连接到第二蛋白质的氨基端或第二蛋白质的羧基端，只要含有包含融合蛋白的第二组件的纳米颗粒在其表面表达HA蛋白的免疫原性部分。

在这些融合蛋白中，第二蛋白质可以直接连接到流感病毒HA蛋白的免疫原性部分，或者第二蛋白质和流感病毒HA蛋白的免疫原性部分可以使用接头连接。如本申请通篇所用，接头(接头序列、接头肽等)是用于共价连接两个具有所需特征(例如结构、自组装能力、引发免疫反应的能力、表位、免疫原性、活性等)氨基酸序列(蛋白质)的短(例如2～20个)氨基酸。优选地，接头长度约为2、3、4、5、6、7、8、9、10、12、14、16、18或20个氨基酸残基。接头序列本身通常没有活性，一般用于使蛋白质构建体的其他部分呈现所需的构象或方向。接头序列通常由小氨基酸残基和/或其连续同残基序列(runs)组成，例如丝氨酸(S)、丙氨酸(A)和甘氨酸(G)，但不排除使用其他氨基酸残基。例如，脯氨酸(P)残基可作为本申请的接头。可用于实施本申请的接头的实例包括但不限于GSGSG(SEQ ID NO:337)、GG、GGG、PPG、GPPG(SEQ ID NO:338)和GGSGGSGS(SEQ ID NO:339)。

应当理解，每个第二组件可以包含一种以上的融合蛋白；即一种以上类型的HA蛋白免疫原性部分与第二蛋白质结合形成融合蛋白。事实上，每个第二组件可以包含任意数量的融合蛋白，最多可达第二组件中第二蛋白质的数量。例如，如果第二个组件由三种第二蛋白质组成，这样的组件可以包含一种、两种或三种融合蛋白，每种这样的融合蛋白包含与HA蛋白的免疫原性部分连接的第二蛋白质。这些免疫原性部分可能来自或可能不来自相同的HA蛋白(即，来自相同类型、组、亚型或流感病毒株的HA蛋白；和/或HA蛋白的不同免疫原性部分，如胞外域、头部、茎和/或稳定的茎)。

此外，虽然单个纳米颗粒内的所有融合蛋白都包含具有相同序列的第二蛋白质，但是同一纳米颗粒内的不同第二组件可以包含具有来自不同类型、组、亚型或流感病毒株的HA蛋白的免疫原性部分的融合蛋白。因此，本申请的纳米颗粒可以包含并共同表达来自一种以上类型、组、亚型或毒株的流感病毒的HA蛋白的免疫原性部分。这种纳米颗粒被称为镶嵌纳米颗粒。镶嵌纳米颗粒可以包含来自尽可能多的流感病毒HA蛋白的免疫原性部分，以引发足以保护被施用者免受所需类型、组、亚型或毒株的病毒的免疫反应。在各种实施方案中，纳米颗粒包含来自至少两种不同流感毒株(即二价纳米颗粒)，或来自至少三种不同流感毒株(即三价纳米颗粒)，或来自至少四种不同流感毒株(即四价纳米颗粒)，或来自至少五种不同流感毒株(即五价纳米颗粒)的HA蛋白的免疫原性部分。在一个实施方案中，纳米颗粒包含来自至少六种不同流感毒株的HA蛋白的免疫原性部分(六价纳米颗粒)。在另一个实施方案中，纳米颗粒是七价或八价的。这种组合的一个非限制性实例是纳米颗粒，其包含来自甲型流感1型HA蛋白、甲型流感2型HA蛋白和乙型流感HA蛋白的免疫原性部分。作为非限制性实例，纳米颗粒可以包含来自H1流感病毒、H2流感病毒、H3流感病毒和乙型流感病毒的HA蛋白的免疫原性部分。在另一个实施方案中，纳米颗粒可以包含(共表达)来自世界卫生组织推荐包含在季节性流感疫苗中的流感病毒的HA蛋白的免疫原性部分(例如，H1甲型流感病毒、H3甲型流感病毒、维多利亚系的乙型流感病毒和山形系的乙型流感病毒)。在一个实施方案中，纳米颗粒可以包含(共表达)来自甲型H1流感病毒、甲型H3流感病毒、维多利亚系乙型流感病毒和山形系乙型流感病毒的HA蛋白的免疫原性部分。

在一个实施方案中，纳米颗粒表达3、4或更多不同HA蛋白的免疫原性部分。在一个实施方案中，不同HA蛋白的免疫原性部分选自甲型流感病毒和乙型流感病毒HA蛋白的免疫原性部分。在另一个实施方案中，不同HA蛋白的免疫原性部分选自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17和H18的HA蛋白的免疫原性部分。在另一个实施方案中，不同HA蛋白的免疫原性部分包含来自以下病毒株的HA蛋白的免疫原性部分，所述病毒株包括但不限于H10N4、H10N5、H10N7、H10N8、H10N9、H11N1、H11N13、H11N2、H11N4、H11N6、H11N8、H11N9、H12N1、H12N4、H12N5、H12N8、H13N2、H13N3、H13N6、H13N7、H14N5、H14N6、H15N8、H15N9、H16N3、H1N1、H1N2、H1N3、H1N6、H1N9、H2N1、H2N2、H2N3、H2N5、H2N7、H2N8、H2N9、H3N1、H3N2、H3N3、H3N4、H3N5、H3N6、H3N8、H3N9、H4N1、H4N2、H4N3、H4N4、H4N5、H4N6、H4N8、H4N9、H5N1、H5N2、H5N3、H5N4、H5N6、H5N7、H5N8、H5N9、H6N1、H6N2、H6N3、H6N4、H6N5、H6N6、H6N7、H6N8、H6N9、H7N1、H7N2、H7N3、H7N4、H7N5、H7N7、H7N8、H7N9、H8N4、H8N5、H9N1、H9N2、H9N3、H9N5、H9N6、H9N7、H9N8和H9N9。在一个实施方案中，HA蛋白的免疫原性部分包含(a)一种甲型流感病毒1型HA、(b)一种甲型流感病毒2型HA和(c)两种乙型流感病毒的免疫原性部分中的任一种的免疫原性部分。在另一个实施方案中，HA蛋白的免疫原性部分包含(a)一种H1HA、(b)一种H3HA和(c)一种或两种乙型流感病毒HA中任一种的免疫原性部分。在进一步的实施方案中，HA蛋白的免疫原性部分包含(a)A/Michigan/45/2015(H1N1)、(b)A/Hong Hong/4801/2014(H3N2)、(c)B/Brisbane/60/2008(维多利亚系)和(d)B/Phuket/3073/2013(山形系)中任一种的免疫原性部分。在一个实施方案中，HA蛋白的免疫原性部分包含(a)A/Michigan/45/2015(H1N1)pdm09-样病毒、(b)A/Singapore/INFIMH-16-0019/2016(H3N2)-样病毒、(c)B/Colorado/06/2017-样(维多利亚系)病毒中任一种的免疫原性部分，并且任选地包括(d)B/Phuket/3073/2013-样(山形系)病毒。在另一个实施方案中，HA蛋白的免疫原性部分包含(a)A/Idaho/07/2018(H1N1)pdm09-样病毒、(b)A/Perth/1008/2019(H3N2)-样病毒(更新的)、(c)B/Colorado/06/2017-样(维多利亚系)病毒(更新的)中任一种的免疫原性部分，并且任选地包括(d)B/Phuket/3073/2013-样(山形系)病毒。

在另一个实施方案中，HA蛋白的免疫原性部分包含以下任一种的免疫原性部分：(a)(2019年(南半球)季节性流感病毒疫苗组合物，(b)A/Michigan/45/2015(H1N1)pdm09-样、(c)A/Singapore/INFIMH-16-0019/2016(H3N2)-样、(d)B/Colorado/06/2017-样(维多利亚系)和(e)B/Phuket/3073/2013-样(山形系)。在另一个实施方案中，HA蛋白的免疫原性部分包含以下任一种的免疫原性部分：(a)2020年(南半球)季节性流感病毒疫苗组合物，(b)A/Brisbane/02/2018(H1N1)pdm09-样、(c)A/South Australia/34/2019(H3N2)-样、(d)B/Washington/02/2019-样(维多利亚系)和(e)B/Phuket/3073/2013-样(山形系)。

在另一个实施方案中，纳米颗粒的形状和/或对称性为四面体、八面体、二十面体、十二面体。在一个具体实施方案中，纳米颗粒的形状和/或对称性为二十面体。

作为镶嵌纳米颗粒的替代物，本申请的组合物可以包含多于一种本申请的纳米颗粒，每种纳米颗粒表达来自相同类型、亚型或株系的流感病毒的HA蛋白的免疫原性部分，其中不同种类的纳米颗粒表达来自不同类型、亚型的流感病毒株系的HA蛋白的免疫原性部分。作为非限制性实施例，组合物可包含表达来自H1流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自H3流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自第一种乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒以及表达来自第二种乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒。这种组合物可以称为“鸡尾酒”。在一个实施方案中，本申请的组合物可以包含多种纳米颗粒种类，每种纳米颗粒种类表达来自世界卫生组织推荐包含在季节性流感疫苗中的一种流感病毒的HA蛋白的免疫原性部分。因此，例如，这种组合物可以包含表达来自甲型H1流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自甲型H3流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自维多利亚系乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒、以及表达来自山形系乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒。

如上所述，本申请的纳米颗粒包含至少一个第二组件，所述第二组件包含与流感血凝素蛋白(即融合蛋白)的免疫原性部分连接的第二蛋白质。融合蛋白的免疫原性部分可以来源于任何流感病毒HA蛋白，只要它能引发针对一种或多种类型、组、亚型或毒株的流感病毒的免疫反应。如本文所用，流感HA蛋白是指全长流感血凝素蛋白或其任何部分，其可用于引发针对流感病毒的免疫应答(即，用作免疫原)。合适的流感HA蛋白的实例在本申请中提供。

在整个公开内容中，用于对流感病毒进行分类的所有命名法都是本领域技术人员惯用的命名法。因此，流感病毒的类型或组是指甲型流感病毒、乙型流感病毒或丙型流感病毒。将病毒的归属为特定类型涉及相应M1(基质)蛋白或NP(核蛋白)的序列差异。甲型流感病毒进一步分为第1组和第2组。这些组进一步分为亚型，即根据病毒的HA蛋白序列对病毒进行分类。目前公认的亚型有H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17和H18。第1组流感亚型为H1、H2、H5、H6、H8、H9、H11、H12、H13、H16、H17和H18。第2组流感亚型是H3、H4、H7、H10、H14和H15。最后，术语“毒株”指的是同一亚型内但其基因组中有小的遗传变异而彼此不同的病毒。

不同流感病毒的血凝素蛋白可能因蛋白突变(插入、缺失)而具有不同的长度。因此，引用相应区域是指另一种在序列、结构和/或功能上与被比较的区域相同或接近相同(例如，至少95％相同、至少98％、至少99％相同或100％相同)的HA蛋白的区域。例如，关于血凝素蛋白的茎区，另一个血凝素蛋白中的相应区域可能不具有相同的残基数量，但是将具有几乎相同的序列并将执行相同的功能。为了更好地阐明病毒之间的序列比较，本领域技术人员使用编号系统，其将氨基酸位置与参考序列相关联。除非另有说明，本文提及的血凝素蛋白中的氨基酸使用H3编号系统。因此，来自不同流感毒株的血凝素蛋白中的相应氨基酸残基，就其与蛋白质的N-末端氨基酸的距离而言，可能不具有相同的残基数。例如，使用H3编号系统，在A/New Caledonia/20/1999(1999NC,H1)中提到的残基100并不指从N端氨基酸算起的第100个氨基酸残基。相反，使用H3编号系统，A/New Caledonia/20/1999(1999NC,H1)的残基100指来自H11999 NC流感的HA中与H3N2流感毒株的残基100对齐的氨基酸。在这种情况下，H1 NC 1999流感中的W93与H3N2中的W100一致，因此A/NewCaledonia/20/1999(1999NC,H1)中的残基93指的是W100。

如本文所用，术语“免疫原性的”、“作为免疫原”等是指特定蛋白质或其特定区域引发自身免疫应答的能力，或对包含与特定蛋白质高度同一性的氨基酸序列的蛋白质的免疫应答的能力。根据本申请，具有高度序列同源性的两种蛋白质具有至少60％、65％、70％、75％、80％、85％、87％、90％、92％、93％、94％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列。确定两个氨基酸序列或核苷酸序列之间同一性百分比的方法是本领域公知的。

用于引发免疫反应的HA蛋白免疫原性区域的一个实例是表位。流感病毒HA蛋白的表位指的是HA蛋白的一部分，其可以引发针对至少同源流感病毒株，即HA来源的病毒株的免疫应答。表位也可引发针对异源流感病毒株，即具有与免疫原的HA相似但序列不同的HA蛋白的类型、组、亚型或病毒株的抗体反应。所述表位优选引发广泛的异源亚型保护性反应。所述表位优选引发中和抗体。

如本文所用，针对本申请的纳米颗粒或疫苗而形成的免疫应答是指受试者对流感病毒HA蛋白的体液和/或细胞免疫应答的强化。为了本申请的目的，“体液免疫应答”是指由抗体分子介导的免疫应答，包括分泌型(IgA)或IgG分子，而“细胞免疫应答”是由T淋巴细胞和/或其他白细胞介导的免疫应答。细胞免疫的一个方面涉及细胞毒性T细胞的免疫原特异性反应。CTL对肽抗原具有特异性，所述肽抗原与主要组织相容性复合体(MHC)编码的蛋白质相关联，并在细胞表面表达。CTL有助于诱导和促进细胞内微生物的破坏，或诱导和促进感染此类微生物的细胞的裂解。细胞免疫的另一个方面涉及辅助性T细胞的抗原特异性反应。辅助性T细胞有助于刺激非特异性效应细胞的功能，并使其活性集中于对抗表面表达与MHC分子相关的肽抗原的细胞。细胞免疫反应也指由活化的T细胞和/或其他白细胞(包括源自CD4+和CD8+T细胞的那些细胞)产生的细胞因子、趋化因子和其他此类分子。

因此，免疫反应可以是刺激CTL和/或辅助性T细胞的产生或活化的反应。还可以刺激趋化因子和/或细胞因子的产生。本申请的纳米颗粒也可以引发抗体介导的免疫反应。因此，免疫反应可能包括以下一种或多种效应：B细胞产生抗体(如IgA或IgG)；和/或特异性针对流感病毒HA蛋白的抑制性、细胞毒性或辅助性T细胞和/或T细胞的激活。这些反应可用于中和传染性(例如，抗体依赖性保护)，和/或介导抗体-补体或抗体依赖性细胞毒性(ADCC)，以向免疫受试者提供保护。这种反应可以使用本领域熟知的标准免疫测定法和中和测定法来检测。本申请的纳米颗粒蛋白质构建体中的HA蛋白优选应用引发广泛免疫应答的免疫原性部分。如本文所用，广义免疫应答是指识别一种以上类型、组、亚型和/或毒株的流感病毒的免疫反应(例如抗体、T细胞等)。

如本文所用，术语抗原性的、抗原性等是指引发抗体或一组抗体并被其结合的蛋白质。类似地，蛋白质的抗原部分是被抗体或一组抗体识别的任何部分。抗体对HA蛋白的识别是指抗体选择性地与HA蛋白结合。如本文所用，短语“选择性结合(selectivelybinds)”、“选择性结合(selectively binding)”等是指抗体优先结合HA蛋白的能力，而不是结合与HA无关的蛋白或样品或分析中的非蛋白组分。“优先结合”HA的抗体是结合HA但不显著结合样品或分析中可能存在的其他分子或成分的抗体。显著结合被认为是例如抗HA抗体与非HA分子的结合，其具有足够大的亲和力或亲合力以干扰检测和/或测定样品中抗流感抗体或HA蛋白水平的能力。样品或分析中可能存在的其他分子和化合物的实例包括但不限于非HA蛋白，例如白蛋白、脂质和碳水化合物。如本文所用，非HA蛋白是与本文公开的流感HA蛋白序列具有小于40％序列同源性的氨基酸序列的蛋白。

如本文所用，“中和抗体”是指阻止流感病毒完成一轮复制的抗体。一轮复制指的是病毒的生命周期，从病毒附着到宿主细胞开始，到新形成的病毒从宿主细胞出芽结束。该生命周期包括但不限于附着于细胞、内化、裂解和重排、病毒膜与内体膜的融合、病毒核糖核蛋白释放到细胞质中、形成新的病毒颗粒以及病毒颗粒从宿主细胞膜出芽的步骤。中和抗体是抑制一个或多个上述步骤的抗体。

如本文所用，“广泛中和抗体”是中和一种以上类型、组、亚型和/或毒株的流感病毒的抗体。例如，针对甲型流感病毒的HA蛋白诱导的广泛中和抗体可以中和乙型或丙型流感病毒。作为另一个实例，针对来自第1组流感病毒的HA蛋白诱导的广泛中和抗体可以中和第2组流感病毒。作为另一个实例，从一种病毒亚型或毒株诱导的广泛中和抗体可以中和另一种病毒亚型或毒株。例如，针对来自H1流感病毒的HA蛋白诱导的广泛中和抗体可以中和来自一个或多个亚型的病毒，例如H2、H3、H4、H5、H6、H7、H8、H8、H10、H11、H12、H13、H14、H15、H16、H17或H18流感病毒。

在本申请的纳米颗粒中，每个第二组件包含多个第二蛋白质，其中至少一个第二组件包含融合蛋白，所述融合蛋白包含与HA蛋白的免疫原性部分连接的相同的第二蛋白质。在各种实施方案中，HA蛋白的免疫原性部分可以包含完整的HA蛋白、HA胞外域、HA头部区域、HA茎区域、稳定的HA蛋白茎、免疫原性部分、变体(如本文所述)及其组合。

在这些融合蛋白中，免疫原性部分可以选自甲型流感病毒、乙型流感病毒和丙型流感病毒的流感病毒。在这些融合蛋白中，HA蛋白的免疫原性部分可以选自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17、H18流感病毒和乙型流感病毒。

在这些融合蛋白中，HA蛋白的免疫原性部分可以包含或由至少6个、8个、10个、25个、50个、75个、100个、50个、200个、250个、300个、350个、400个、450个或500个或更多连续氨基酸残基组成，所述氨基酸残基与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong_Kong/4801/2014(H3N2)、A/Hong_Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig_Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的流感病毒的HA蛋白序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。

流感病毒HA蛋白的胞外域区域包含从分泌信号肽之后到HA蛋白跨膜结构域之前的所有氨基酸残基、或(大约)甲型流感病毒HA蛋白的氨基酸残基1-517(H3编号系统)。胞外域的一个实例由SEQ ID NO:1表示，其代表来自流感病毒A/New Caledonia/20/1999(H1N1)的HA蛋白的胞外域。各种HA蛋白胞外域的其它实例为本申请的SEQ ID NO:4、7、10、13、16、19、22、25、28、31、34和37。所公开的HA蛋白胞外域区域的列表并非旨在将本申请限制为所述序列，而是旨在向本领域技术人员提供关于HA蛋白胞外域的性质和结构的指导，以及来自各种流感HA蛋白的胞外域的实例。任何流感病毒HA蛋白胞外域区域都可用于实施本申请。

因此，在一个实施方案中，本申请的纳米颗粒包含至少一个第二组件，所述第二组件包含融合蛋白，所述融合蛋白包含与流感病毒HA蛋白的胞外域区域的免疫原性部分连接的第二蛋白质。在这些融合蛋白中，HA蛋白的免疫原性部分可以来自甲型、乙型或丙型流感病毒的HA蛋白。例如，免疫原性部分可以来自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17或H18流感病毒的HA蛋白。在这些融合蛋白中，免疫原性部分可以包含或由至少6个、8个、10个、25个、50个、75个、100个、50个、200个、250个、300个、350个、400个、450个或500个或更多连续氨基酸残基组成，所述氨基酸残基来自与选自A/NewCaledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/HongKong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的胞外域区域的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。在这些融合蛋白中，HA蛋白的免疫原性部分可以包含或由至少6个、8个、10个、25个、50个、75个、100个、50个、200个、250个、300个、350个、400个、450个或500个连续的氨基酸残基组成，所述氨基酸残基来自与选自SEQ ID NO：1、4、7、10、13、16、19、22、25、28、31、34和37的氨基酸序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。

已知流感病毒HA蛋白的胞外域包含不同的区域或结构域。这种区域的实例包括茎部区域和球状头部区域。本申请的纳米颗粒可以包含含有融合蛋白的第二组件，所述融合蛋白包含来自流感病毒HA蛋白头部区域的免疫原性部分。头部区域(大约)包括甲型流感病毒HA蛋白(H3编号系统)的氨基酸残基52-277，其仅由HA1蛋白的主要部分组成，并包括两个结构域：受体结合结构域(RBD)和残留酯酶亚结构域。球状头部区域的一个实例是如SEQ IDNO:1所示的氨基酸59-291表示，其代表来自流感病毒A/New Caledonia/20/1999(H1N1)的HA蛋白的胞外域。A/New Caledonia/20/1999(H1N1)的HA蛋白的头部区域在本文中如SEQID NO:2所示。可用于本申请的蛋白质构建体和方法的其它HA头部区域是其它HA蛋白中对应于流感病毒A/New Caledonia/20/1999(H1N1)的HA蛋白头部区域的区域(即对应于SEQID NO:1所示的氨基酸59-291的区域)。各种HA蛋白的头部区域的实例如本申请的SEQ IDNO:5、8、11、14、17、20、23、26、29、32、35和38所示。所公开的HA蛋白头部区域的列表并非旨在将本申请限制为所述序列，而是旨在向本领域技术人员提供关于头部区域的性质和结构的指导，以及来自各种流感病毒HA蛋白的头部区域的实例。任何流感病毒HA蛋白头部区域都可用于实施本发明。

因此，在一个实施方案中，本申请的纳米颗粒包含至少一个包含融合蛋白的第二组件，所述融合蛋白包含与来自流感病毒HA蛋白头部区域的免疫原性部分连接的第二蛋白质。在所述融合蛋白中，HA蛋白的免疫原性部分可以来自甲型、乙型或丙型流感病毒HA蛋白的头部区域。例如，免疫原性部分可以来自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17或H18流感病毒的HA蛋白。在这些融合蛋白中，HA蛋白的免疫原性部分可包含或由至少6、8、10、25、50、75、100或200个或更多连续氨基酸残基组成，所述氨基酸残基来自与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/Hong Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的流感病毒的HA蛋白头部区域序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。在这些融合蛋白中，HA蛋白的免疫原性部分可以包含或由来自与SEQID NO:2、5、8、11、14、17、20所示的氨基酸序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的至少6、8、10、25、50、75、100或200个或更多连续氨基酸残基组成。

虽然流感病毒HA蛋白的头部区域完全位于流感病毒HA蛋白的HA1部分，但茎区域包括来自HA1和HA2部分的氨基酸序列。根据本申请，构成流感病毒HA蛋白的茎部的氨基酸残基是去除头部区域序列后剩余的氨基酸残基。例如，来自流感病毒A/New Caledonia/20/1999(1999NC,H1)(如SEQ ID NO:1所示)的HA蛋白的茎区由SEQ ID NO:1所示的氨基酸残基1-58和292-517组成。类似地，来自其他流感病毒HA蛋白的茎区指的是流感病毒A/NewCaledonia/20/1999(1999NC,H1)(如SEQ ID NO:1所示)的HA蛋白中对应于氨基酸残基1-58以及氨基酸残基292-517的氨基酸残基。这种公开并不旨在将本申请限制于本文公开的特定HA蛋白序列，而是旨在向本领域技术人员提供关于茎区的性质和结构的指导，并且作为流感HA蛋白的茎区的实例。来自任何流感病毒HA蛋白茎区的氨基酸序列可用于实施本申请。

因此，在一个实施方案中，本申请的纳米颗粒包含至少一个包含融合蛋白的第二组件，所述融合蛋白包含与流感病毒HA蛋白的茎区的免疫原性部分连接的第二蛋白。在这些融合蛋白中，来自HA蛋白茎区的免疫原性部分可以来自甲型、乙型或丙型流感病毒的HA蛋白。例如，免疫原性部分可以来自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17或H18流感病毒的HA蛋白。在这些融合蛋白中，HA蛋白的茎区的免疫原性部分可以包含或由来自HA蛋白的茎区的至少6、8、10、25、50、75、100或200个或更多连续氨基酸残基组成，所述氨基酸残基包含与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/Hong Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)流感病毒的HA蛋白序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列。

在这些融合蛋白中，HA蛋白的免疫原性部分可包含或由至少6个、8个、10个、25个或50个或更多个连续氨基酸残基组成，所述氨基酸残基来自与SEQ ID NO:1的氨基酸残基1-58、SEQ ID NO:4的氨基酸残基1-58、SEQ ID NO:7的氨基酸残基1-67、SEQ ID NO:10的氨基酸残基1-68、SEQ ID NO:13的氨基酸残基1-68、SEQ ID NO:16的氨基酸残基1-5、SEQ IDNO:19的氨基酸残基1-57、SEQ ID NO:22的氨基酸残基1-57、SEQ ID NO:25的氨基酸残基1-59、SEQ ID NO:28的氨基酸残基1-59、SEQ ID NO:31的氨基酸残基1-58、SEQ ID NO:34的氨基酸残基1-67或SEQ ID NO:37的氨基酸残基1-68至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。

在这些融合蛋白中，HA蛋白的免疫原性部分可以包含或由至少6个、8个、10个、25个、50个、75个、100个或200个或更多个连续氨基酸残基组成，所述氨基酸残基与SEQ IDNO:1的氨基酸残基292-517、SEQ ID NO:4的氨基酸残基293-515、SEQ ID NO:7的氨基酸残基294-517、SEQ ID NO:10的氨基酸残基311-539、SEQ ID NO:13的氨基酸残基310-538、SEQID NO:16的氨基酸残基289-518、SEQ ID NO:19的氨基酸残基291-520、SEQ ID NO:22的氨基酸残基294-523、SEQ ID NO:25的氨基酸残基287-516、SEQ ID NO:28的氨基酸残基287-516、SEQ ID NO:31的氨基酸残基288-518、SEQ ID NO:34的氨基酸残基294-525或SEQ IDNO:37的氨基酸残基308-537至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的序列。

在本申请的第二组件融合蛋白中使用的HA茎区的免疫原性部分需要呈现类似于在野生型流感病毒颗粒中或在感染流感病毒的细胞中观察到的茎区的三维结构。然而，如上所述，茎区是由位于头部区域侧面的氨基酸序列构成的，因此，去除头部区域序列会破坏HA蛋白的三维结构。为了克服这个问题，本申请的融合蛋白可以包含HA蛋白的免疫原性部分，该部分包含稳定的HA蛋白茎(也称为稳定的蛋白茎、无头HA蛋白茎、无头HA蛋白或仅HA蛋白茎)，其是包含两个显著修饰的流感HA蛋白。在第一次修饰中，头部区域氨基酸序列被去除，头部区域两侧的茎区域序列共价结合在一起，从而稳定无头HA蛋白茎。在这些无头构建体中，去除的头部区域侧翼的氨基酸序列可以直接连接(即上游侧翼序列羧基末端的氨基酸残基与紧邻头部区域下游的氨基酸序列氨基末端的氨基酸残基共价连接)或通过肽接头序列连接。为了举例说明和阐明这种结构，可以通过去除如SEQ ID NO:1所示的A/NewCaledonia/20/1999(1999NC,H1)病毒的HA蛋白的氨基酸残基59-291，然后将58位的亮氨酸残基共价连接到292位的天冬氨酸残基，使其无头。

除了头部区域的缺失，还可以在茎序列中进行一个或多个突变，以进一步稳定无头HA蛋白。特别是，在HA序列中不直接共价连接的氨基酸残基(即，在线性HA序列中彼此不靠近的氨基酸残基；例如，间隔大于5个残基)，但在折叠的野生型HA蛋白的三维空间中非常接近，并且不相互作用或相互作用弱，可以被其他氨基酸残基取代以产生相互作用，或增加折叠稳定蛋白茎中残基之间相互作用的强度。例如，在折叠的HA蛋白中，与流感ANewCaledonia/20/1999(H1)的K394和E446[K51(HA2)和E103(HA2)]对应的氨基酸残基(与SEQID NO:3的K1和E53对应)形成盐桥，有助于稳定折叠的蛋白质。通过用合适的氨基酸取代这些赖氨酸和谷氨酸残基，可以增强两个氨基酸残基之间的相互作用，从而提高分子的稳定性，并允许对其进行更广泛的操作。使用来自任何流感病毒的HA蛋白，利用所选HA蛋白中的相应序列和位置，可以制备类似的稳定化蛋白茎。例如，来自流感病毒A/Michigan/45/2015(H1N1)的HA蛋白，其胞外域如SEQ ID NO:4所示，可以通过去除氨基酸残基59-292，然后将58位的亮氨酸残基共价连接到293位的天冬氨酸残基而变成无头HA蛋白。生产稳定的蛋白茎的其它方法公开在公开号为WO/2015/183969和WO/2018/045308的PCT专利中，其通过引用并入本文。

因此，在一个实施方案中，本申请的纳米颗粒包含至少一个包含融合蛋白的第二组件，所述融合蛋白包含与流感病毒HA稳定的蛋白茎连接的第二蛋白质。在这些融合蛋白中，HA蛋白的免疫原性部分可以包含或由稳定的蛋白茎组成，所述蛋白茎来源于甲型、乙型或丙型流感病毒的HA蛋白。例如，这些融合蛋白中HA蛋白的免疫原性部分可以包含或由稳定的蛋白茎组成，所述蛋白茎来源于来自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17或H18流感病毒的HA蛋白。作为另一个实例，这些融合蛋白中HA蛋白的免疫原性部分可以包含或由HA蛋白组成，所述HA蛋白包含与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/Hong Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/BrevigMission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的流感病毒的HA蛋白茎序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列。

在一个具体的实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:1所示的氨基酸1-58至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，所述氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:1所示的氨基酸292-517至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于如SEQ ID NO:2所示氨基酸59-291的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的茎蛋白包含与SEQ ID NO:4所示的氨基酸1-58至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到到与SEQ ID NO:4所示的氨基酸293-515至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ ID NO:5所示氨基酸59-292的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:7所示的氨基酸1-67至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:7所示的氨基酸294-517至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:8所示的氨基酸68-293的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:10所示的氨基酸1-678至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:10所示的氨基酸311-539至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:11所示氨基酸68-310的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:13所示的氨基酸1-68至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:13所示的氨基酸310-538至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:14所示氨基酸69-309的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:16所示的氨基酸1-56至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:16所示的氨基酸289-518至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:17所示氨基酸57-288的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:19所示的氨基酸1-57至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:19所示的氨基酸291-520至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:20所示氨基酸58-290的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:22所示的氨基酸1-57至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:22所示的氨基酸294-523至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:23所示氨基酸58-293的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:25所示的氨基酸1-59至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:25所示的氨基酸287-516至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:26所示氨基酸60-286的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:28所示的氨基酸1-59至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:28所示的氨基酸287-516至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:29所示氨基酸60-286的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:31所示的氨基酸1-58至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:31所示的氨基酸288-518至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:32所示氨基酸59-287的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:34所示的氨基酸1-67至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:34所示的氨基酸294-525至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:35所示氨基酸68-293的氨基酸序列。

在另一个具体实施例中，免疫原性HA部分可以包含或由稳定的蛋白茎组成，所述稳定的蛋白茎包含与SEQ ID NO:37所示的氨基酸1-68至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列，该氨基酸序列直接或通过肽接头共价连接到与SEQ ID NO:37所示的氨基酸308-537至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列上，使得稳定的蛋白茎缺少对应于SEQ IDNO:38所示氨基酸69-307的氨基酸序列。

除了缺乏头部区域之外，本文所述的稳定的蛋白茎还可以包含一个或多个突变，这些突变使稳定的HA蛋白茎更加稳定。因此，本申请的第二组件融合蛋白可以包含稳定的HA蛋白茎，其缺少对应于HA蛋白头部区域的氨基酸序列，并且在稳定的蛋白茎中的氨基酸残基是除赖氨酸之外的氨基酸残基，对应于如SEQ ID NO：3、6、9、18、21、24、27、30、33或36所示的任一项K1的位置处的氨基酸残基。此外或进一步地，在稳定的蛋白茎中的氨基酸残基是除谷氨酰胺之外的氨基酸残基，对应于如SEQ ID NO：3、6、9、18、21、24、27、30、33或36所示的任一项E53的位置处的氨基酸残基。

在这些稳定的HA蛋白茎中，这两种取代的氨基酸残基(K1和/或E53)可以分别为选自甲硫氨酸、亮氨酸、异亮氨酸和谷氨酰胺的氨基酸残基。在这些稳定的HA蛋白茎中，这两种取代的氨基酸残基(K1和/或E53)被氨基酸残基取代，从而增加了折叠蛋白质中这些残基之间相互作用的强度。

如上所述，除了头部区域的缺失和如SEQ ID NO:3、6、9、18、21、24、27、30、33或36所示的序列中任何一项对应于K1和/或E53位置的氨基酸残基的取代之外，稳定的蛋白茎也可能缺乏内环结构序列。内环结构序列的一个实例是SEQ ID NO:1所示的氨基酸残基403-435。所有流感病毒HA蛋白均含有一个内环结构序列，对应于SEQ ID NO:1所示的氨基酸残基403-435。茎区的缺失导致内环结构序列侧翼的氨基酸序列共价连接(即上游侧翼序列羧基末端的氨基酸残基与紧邻头部区域下游的氨基酸序列氨基末端的氨基酸残基共价连接)。为了举例说明和阐明这种结构，将SEQ ID NO:1所示的流感病毒A/New Caledonia/20/1999(1999NC,H1)HA蛋白中的内环结构序列去除，导致402位的甲硫氨酸直接或通过肽接头共价连接到436位的苏氨酸氨基酸残基上。

因此，本申请的融合蛋白可以包含HA蛋白的免疫原性部分，其中所述免疫原性部分包含或由缺少对应于HA蛋白头部区域的氨基酸序列的稳定的蛋白茎组成，并且SEQ IDNO:1所示稳定的蛋白茎中对应于M402的氨基酸残基直接或通过肽接头共价连接到SEQ IDNO:1所示的稳定的蛋白茎中对应于T436的氨基酸残基，使得稳定的蛋白茎缺少SEQ ID NO:1所示的对应于403-435的氨基酸残基序列。此外，在这些免疫原性HA融合蛋白中，SEQ IDNO:3、6、9、18、21、24、27、30、33或36中所示的任一个稳定的蛋白茎中对应于K1或E53的一个或两个位置的氨基酸残基可以分别是除赖氨酸或谷氨酰胺之外的氨基酸残基。

公开号为WO 2015/183969和WO 2018/045308的国际专利中公开了制备稳定的HA蛋白茎的方法及此类蛋白的实例，这两个文献在此通过引用并入本文。

在一个非限制性实施方案中，本申请的纳米颗粒包含至少一个包含融合蛋白的第二组件，所述融合蛋白包含与流感HA蛋白的免疫原性部分连接的第二蛋白质，其中HA蛋白的免疫原性部分包含或由与选自SEQ ID NO:1、SEQ ID NO:4、SEQ ID NO:7、SEQ ID NO:10、SEQ ID NO:13、SEQ ID NO:16、SEQ ID NO:19、SEQ ID NO:22、SEQ ID NO:25、SEQ ID NO:28、SEQ ID NO:31、SEQ ID NO:34、SEQ ID NO:37、SEQ ID NO:325、SEQ ID NO:326、SEQ IDNO:327、SEQ ID NO:328、SEQ ID NO:329、SEQ ID NO:330、SEQ ID NO:331、SEQ ID NO:332、SEQ ID NO:333、SEQ ID NO:334、SEQ ID NO:335和SEQ ID NO:336的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

在一个非限制性实施方案中，融合蛋白可以包含或由与选自SEQ ID NO:91、SEQID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ IDNO:115、SEQ ID NO:117、SEQ ID NO:119、SEQ ID NO:121、SEQ ID NO:123、SEQ ID NO:125、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ IDNO:137、SEQ ID NO:139、SEQ ID NO:141、SEQ ID NO:143、SEQ ID NO:145、SEQ ID NO:147、SEQ ID NO:149、SEQ ID NO:151、SEQ ID NO:153、SEQ ID NO:155、SEQ ID NO:157、SEQ IDNO:159、SEQ ID NO:161、SEQ ID NO:163、SEQ ID NO:165、SEQ ID NO:167、SEQ ID NO:169、SEQ ID NO:171、SEQ ID NO:173、SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ IDNO:181、SEQ ID NO:183、SEQ ID NO:185、SEQ ID NO:187、SEQ ID NO:189、SEQ ID NO:191、SEQ ID NO:193、SEQ ID NO:195、SEQ ID NO:197、SEQ ID NO:199、SEQ ID NO:201、SEQ IDNO:203、SEQ ID NO:205、SEQ ID NO:207、SEQ ID NO:209、SEQ ID NO:211、SEQ ID NO:213、SEQ ID NO:215、SEQ ID NO:217、SEQ ID NO:219、SEQ ID NO:221、SEQ ID NO:223、SEQ IDNO:225、SEQ ID NO:259、SEQ ID NO:261、SEQ ID NO:263、SEQ ID NO:265和SEQ ID NO:340-346的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中融合蛋白非共价相互作用形成第二组件。

在可与本文任何实施方案组合的具体实施方案中，融合蛋白可包含或由与选自SEQ ID NO:340-346的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

A/Brisbane/02/2018HA 1-676Y98F no lkr dn5B.SA

MKAILVVLLYTFTTANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLGGVAPLHLGKCNIAGWILGNPECESLSTARSWSYIVETSNSDNGTCFPGDFINYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLNQTYINDKGKEVLVLWGIHHPPTTADQQSLYQNADAYVFVGTSRYSKKFKPEIATRPKVRDREGRMNYYWTLVEPGDKITFEATGNLVVPRYAFTMERNAGSGIIISDTPVHDCNTTCQTAEGAINTSLPFQNVHPVTIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDKITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRNQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREKIDGVSAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:340)

A/Idaho/07/2018HA 1-676Y98F no lkr dn5B.SA

MKAILVVLLYTFTTANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTARSWSYIVETSNSDNGTCFPGDFINYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLNQTYINDKGKEVLVLWGIHHPPTTADQQSLYQNADAYVFVGTSRYSKKFKPEIATRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFTMERNAGSGIIISDTPVHDCNTTCQTPEGAINTSLPFQNVHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDKITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRNQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREKIDGVSAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:341)

A/Switzerland/3330/2017 HA 1-676 Y98F no lkr dn5B.SA

MKAILVVLLYTFTTANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTARSWSYIVETSNSDNGTCFPGDFINYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLNQTYINDKGKEVLVLWGIHHPPTTADQQSLYQNADAYVFVGTSRYSKKFKPEIATRPKVRDQEGRMNYYWTLVDPGDKITFEATGNLVVPRYAFTMERDAGSGIIISDTPVHDCNTTCQTPEGAINTSLPFQNVHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDKITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRNQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREKIDGVSAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:342)

A/Perth/1008/2019 HA 1-676 Y98F GG dn5B.SA

MKTIIALSYILCLVFAQKIPGNDNSTATLCLGHHAVPNGTIVKTITNDRIEVTNATELVQNSSIGEICDSPHQILDGGNCTLIDALLGDPQCDGFQNKKWDLFVERSRAYSNCFPYDVPDYASLRSLVASSGTLEFKNESFNWAGVKQNGTSSACIRGSSSSFFSRLNWLTHLNYTYPALNVTMPNKEQFDKLYIWGVHHPGTDKDQIFLYARSSGRITVSTRRSQQAVIPNIGFRPRIRDIPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGKSSIMRSDAPIGKCKSECITPNGSIPNDKPFQNVNRITYGACPRYVKQSTLKLATGMRNVPEKQTRGIFGAIAGFIENGWEGMMDGWYGFRHQNSEGRGQAADLKSTQAAIDQINGKLNRLIGKTNEKFHQIEKEFSEVEGRVQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTKKQLRENAEDMGNGCFKIYHKCDNACIGSIRNGTYDHNVYRDEALNNRFQIKGVGGSAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:343)

A/Wisconsin/549/2019 HA 1-676 Y98F GG dn5B.SA

MKTIIALSCILCLVFAQKIPGNDNSTATLCLGHHAVPNGTIVKTITNDRIEVTNATELVQNSSIGEICDSPHQILDGENCTLIDALLGDPQCDGFQNKKWDLFVERNKAYSNCFPYDVPDYASLRSLVASSGTLEFNNESFNWAGVTQNGKSSSCIRGSKSSFFSRLNWLTHLNSKYPALNVTMPNNEQFDKLYIWGVHHPGTDKDQISLYAQSSGRITVSTKRSQQAVIPNIGSRPRIRDIPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGKSSIMRSDAPIGKCKSECITPNGSIPNDKPFQNVNRITYGACPRYVKQSTLKLATGMRNVPERQTRGIFGAIAGFIENGWEGLVDGWYGFRHQNSEGRGQAADLKSTQAAIDQINGKLNRLIGKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTKKQLRENAEDVGNGCFKIYHKCDNACMGSIRNGTYDHNVYRDEALNNRFQIKGVGGSAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:344)

B/Colorado/06/2017HA1-674nolkrdn5B.SA

MKAIIVLLMVVTSSADRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSHFANLKGTETRGKLCPKCLNCTDLDVALGRPKCTGKIPSARVSILHEVRPVTSGCFPIMHDRTKIRQLPNLLRGYEHVRLSTHNVINAEGAPGGPYKIGTSGSCPNITNGNGFFATMAWAVPDKNKTATNPLTIEVPYVCTEGEDQITVWGFHSDNETQMAKLYGDSKPQKFTSSANGVTTHYVSQIGGFPNQTEDGGLPQSGRIVVDYMVQKSGKTGTITYQRGILLPQKVWCASGRSKVIKGSLPLIGEADCLHEKYGGLNKSKPYYTGEHAKAIGNCPIWVKTPLKLANGTKYRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADLKSTQEAINKITKNLNSLSELEVKNLQRLSGAMDELHNEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPSAVEIGNGCFETKHKCNQTCLDKIAAGTFDAGEFSLPTFDSLNITAASAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:345)

B/Phuket/3073/2013 HA 1-674 no lkr dn5B.SA

MKAIIVLLMVVTSNADRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSYFANLKGTRTRGKLCPDCLNCTDLDVALGRPMCVGTTPSAKASILHEVRPVTSGCFPIMHDRTKIRQLPNLLRGYEKIRLSTQNVIDAEKAPGGPYRLGTSGSCPNATSKIGFFATMAWAVPKDNYKNATNPLTVEVPYICTEGEDQITVWGFHSDNKTQMKSLYGDSNPQKFTSSANGVTTHYVSQIGDFPDQTEDGGLPQSGRIVVDYMMQKPGKTGTIVYQRGVLLPQKVWCASGRSKVIKGSLPLIGEADCLHEEYGGLNKSKPYYTGKHAKAIGNCPIWVKTPLKLANGTKYRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADLKSTQEAINKITKNLNSLSELEVKNLQRLSGAMDELHNEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPSAVDIGNGCFETKHKCNQTCLDRIAAGTFNAGEFSLPTFDSLNITAASAEEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKALELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE(SEQ ID NO:346)

在一个非限制性实施方案中，纳米颗粒包括：

(a)多个第一组件，每个第一组件包含多个相同的第一蛋白质；

(b)多个第二组件，每个第二组件包含多个相同的第二蛋白质；

其中每个第一蛋白质包含或由与选自SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ IDNO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQ ID NO:65、SEQ ID NO:67、SEQ IDNO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ IDNO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:347、SEQ ID NO:348和SEQID NO:349所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第一蛋白质非共价相互作用形成第一组件；

其中每个第二蛋白质包含或由与SEQ ID NO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个第二蛋白质非共价相互作用形成第二组件；

其中至少一个第二组件包含与流感病毒HA蛋白的胞外域连接的第二蛋白质，从而形成融合蛋白；

其中多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒；并且

其中纳米颗粒在纳米结构的外表面表达流感病毒HA蛋白胞外域。

在这些纳米颗粒中，胞外域可以包含或由与选自SEQ ID NO:1、SEQ ID NO:4、SEQID NO:7、SEQ ID NO:10、SEQ ID NO:13、SEQ ID NO:16、SEQ ID NO:19、SEQ ID NO:22、SEQID NO:25、SEQ ID NO:28、SEQ ID NO:31、SEQ ID NO:34和SEQ ID NO:37所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

在这些纳米颗粒中，融合蛋白可以包含或由与选自SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQID NO:117、SEQ ID NO:119、SEQ ID NO:121、SEQ ID NO:123、SEQ ID NO:125、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137、SEQID NO:139、SEQ ID NO:141、SEQ ID NO:143、SEQ ID NO:145、SEQ ID NO:147、SEQ ID NO:149、SEQ ID NO:151、SEQ ID NO:153、SEQ ID NO:155、SEQ ID NO:157、SEQ ID NO:159、SEQID NO:161、SEQ ID NO:163、SEQ ID NO:165、SEQ ID NO:167、SEQ ID NO:169、SEQ ID NO:171、SEQ ID NO:173、SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ ID NO:181、SEQID NO:183、SEQ ID NO:185、SEQ ID NO:187和SEQ ID NOS:340-346所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

另一个实施方案为一种纳米颗粒，包括：

(a)多个第一组件，每个第一组件包含多个相同的第一蛋白质；

(b)多个第二组件，每个第二组件包含多个相同的第二蛋白质；

其中每个第一蛋白质包含或由与选自SEQ ID NO:227、SEQ ID NO:229、SEQ IDNO:231、SEQ ID NO:233、SEQ ID NO:235、SEQ ID NO:237、SEQ ID NO:239、SEQ ID NO:241和SEQ ID NO:243所示的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中，多个相同的第一蛋白质非共价相互作用以形成第一组件；

其中每个第二蛋白质包含或由与选自SEQ ID NO:245、SEQ ID NO:247、SEQ IDNO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255和SEQ ID NO:257所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成；

其中至少一个第二组件包含与流感病毒HA蛋白的胞外域连接的第二蛋白质，从而形成融合蛋白；

其中多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒；并且

其中纳米颗粒在纳米结构的外表面表达流感病毒HA蛋白胞外域。

在这些纳米颗粒中，胞外域可以包含或由选自SEQ ID NO:1、SEQ ID NO:4、SEQ IDNO:7、SEQ ID NO:10、SEQ ID NO:13、SEQ ID NO:16、SEQ ID NO:19、SEQ ID NO:22、SEQ IDNO:25、SEQ ID NO:28、SEQ ID NO:31、SEQ ID NO:34和SEQ ID NO:37所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

在这些纳米颗粒中，融合蛋白可以包含或由与选自SEQ ID NO:259、SEQ ID NO:261、SEQ ID NO:263、SEQ ID NO:265所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个融合蛋白相互作用形成第二组件。

本申请的一个实施方案是包含多个第一组件和多个第二组件的纳米颗粒，每个第一组件包含多个相同的第一蛋白质，每个第二组件包含多个相同的第二蛋白质，其中第一蛋白质和第二蛋白质包含或由氨基酸序列组成，所述氨基酸序列分别与选自表2中的第一和第二蛋白质配对的第一和第二蛋白质的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同，其中至少一个第二组件包含连接到流感血凝素(HA)蛋白质的胞外域的第二蛋白质，从而形成融合蛋白。在这些纳米颗粒中，胞外域可以包含或由与选自SEQ ID NO:1、SEQ ID NO:4、SEQ ID NO:7、SEQ ID NO:10、SEQ ID NO:13、SEQ IDNO:16、SEQ ID NO:19、SEQ ID NO:22、SEQ ID NO:25、SEQ ID NO:28、SEQ ID NO:31、SEQ IDNO:34和SEQ ID NO:37所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

本申请的一个实施方案是一种纳米颗粒，包括：

((a)多个第一组件，每个第一组件包含多个相同的第一蛋白质；

(b)多个第二组件，每个第二组件包含多个相同的第二蛋白质；

其中每个第一蛋白质包含或由与选自SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ IDNO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:32、SEQ ID NO:65、SEQ ID NO:67、SEQ IDNO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ IDNO:81、SEQ ID NO:83、SEQ ID NO:85、and SEQ ID NO:87、SEQ ID NO:347、SEQ ID NO:348和SEQ ID NO:349所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个相同的第一蛋白质非共价相互作用形成第一组件；

其中每个第二蛋白质包含或由与SEQ ID NO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个第二蛋白质非共价相互作用形成第二组件；

其中至少一个第二组件包含与流感病毒HA稳定的蛋白茎连接的第二蛋白质，从而形成融合蛋白；

其中多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒；并且

其中纳米颗粒在纳米结构的外表面表达流感病毒HA稳定的茎。

在这些纳米颗粒中，稳定的蛋白茎可以包含或由与选自SEQ ID NO:325、SEQ IDNO:326、SEQ ID NO:327、SEQ ID NO:328、SEQ ID NO:329、SEQ ID NO:330、SEQ ID NO:331、SEQ ID NO:332、SEQ ID NO:333、SEQ ID NO:334、SEQ ID NO:335、SEQ ID NO:336所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

在这些纳米颗粒中，融合蛋白可以包含或由与选自SEQ ID NO:189,SEQ ID NO:191,SEQ ID NO:193,SEQ ID NO:195,SEQ ID NO:197,SEQ ID NO:199,SEQ ID NO:201,SEQID NO:203,SEQ ID NO:205,SEQ ID NO:207,SEQ ID NO:209,SEQ ID NO:211,SEQ ID NO:213,SEQ ID NO:215,SEQ ID NO:217,SEQ ID NO:219,SEQ ID NO:221,SEQ ID NO:223,andSEQ ID NO:225所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个融合蛋白非共价相互作用形成第二组件。

本申请的一个实施方案是纳米颗粒，包括：

(a)多个第一组件，每个第一组件包含多个相同的第一蛋白质；

(b)多个第二组件，每个第二组件包含多个相同的第二蛋白质；

其中每个第一蛋白质包含或由与选自EQ ID NO:227、SEQ ID NO:229SEQ ID NO:231、SEQ ID NO:233、SEQ ID NO:235、SEQ ID NO:237、SEQ ID NO:239、SEQ ID NO:241和SEQ ID NO:243所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成，其中多个第一蛋白质非共价相互作用形成第一组件；

其中每个第二蛋白质包含或由与选自SEQ ID NO:245、SEQ ID NO:247、SEQ IDNO:249、SEQ ID NO:251、SEQ ID NO:253、SEQ ID NO:255和SEQ ID NO:257所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成；

其中至少一个第二组件包含与流感病毒HA稳定的蛋白茎连接的第二蛋白质，从而形成融合蛋白；

其中多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒；并且

其中纳米颗粒在纳米结构的外表面表达流感病毒HA稳定的茎。

在这些纳米颗粒中，稳定的蛋白茎可以包含或由与选自SEQ ID NO:325、SEQ IDNO:326、SEQ ID NO:327、SEQ ID NO:328、SEQ ID NO:329、SEQ ID NO:330、SEQ ID NO:331、SEQ ID NO:332、SEQ ID NO:333、SEQ ID NO:334、SEQ ID NO:335、SEQ ID NO:336所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

本申请的一个实施方案是包含多个第一组件和多个第二组件的纳米颗粒，每个第一组件包含多个相同的第一蛋白质，每个第二组件包含多个相同的第二蛋白质，其中第一蛋白质和第二蛋白质包含或由氨基酸序列组成，所述氨基酸序列分别与选自表2中的第一和第二蛋白质对的第一和第二蛋白质的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同，其中至少一个第二组件包含与流感病毒HA稳定的蛋白茎连接的第二蛋白质，从而形成融合蛋白。在这些纳米颗粒中，稳定的蛋白茎可以包含或由与选自SEQ ID NO:325、SEQ ID NO:326、SEQ ID NO:327、SEQ ID NO:328、SEQ ID NO:329、SEQID NO:330、SEQ ID NO:331、SEQ ID NO:332、SEQ ID NO:333、SEQ ID NO:334、SEQ ID NO:335、SEQ ID NO:336所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

本申请的一个实施方案是一种融合蛋白，所述融合蛋白包含或由与SEQ ID NO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成；所述融合蛋白与流感病毒HA蛋白的免疫原性部分结合。

本申请的一个实施方案是一种蛋白质，所述蛋白质包含或由与选自SEQ ID NO:245,SEQ ID NO:247,SEQ ID NO:249,SEQ ID NO:251,SEQ ID NO:253,SEQ ID NO:255,andSEQ ID NO:257所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成；所述蛋白质与流感病毒HA蛋白的免疫原性部分结合。

在这些包含与流感病毒HA蛋白的免疫原性部分连接的本申请第二蛋白质的氨基酸序列的蛋白质中，免疫原性部分可以是来自任何流感病毒的HA蛋白的免疫原性部分。因此，在一个实施方案中，免疫原性部分包含或由来自HA蛋白的至少6、8、10、25、50、75、100、200、300或400个连续氨基酸残基组成，所述HA蛋白包含与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Hong Kong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/Hong Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的流感病毒的HA蛋白序列至少80％、85％、90％、91％、95％、96％、97％、98％或100％相同的氨基酸序列。

本申请的一个实施方案是一种蛋白质，所述蛋白质包含或由与选自SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ IDNO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、SEQ ID NO:119、SEQ ID NO:121、SEQ ID NO:123、SEQ IDNO:125、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137、SEQ ID NO:139、SEQ ID NO:141、SEQ ID NO:143、SEQ ID NO:145、SEQ IDNO:147、SEQ ID NO:149、SEQ ID NO:151、SEQ ID NO:153、SEQ ID NO:155、SEQ ID NO:157、SEQ ID NO:159、SEQ ID NO:161、SEQ ID NO:163、SEQ ID NO:165、SEQ ID NO:167、SEQ IDNO:169、SEQ ID NO:171、SEQ ID NO:173、SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ ID NO:181、SEQ ID NO:183、SEQ ID NO:185、SEQ ID NO:187、SEQ ID NO:189、SEQ IDNO:191、SEQ ID NO:193、SEQ ID NO:195、SEQ ID NO:197、SEQ ID NO:199、SEQ ID NO:201、SEQ ID NO:203、SEQ ID NO:205、SEQ ID NO:207、SEQ ID NO:209、SEQ ID NO:211、SEQ IDNO:213、SEQ ID NO:215、SEQ ID NO:217、SEQ ID NO:219、SEQ ID NO:221、SEQ ID NO:223和SEQ ID NO:225所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

本申请的一个实施方案是一种蛋白质，所述蛋白质包含或由与选自SEQ ID NO:259、SEQ ID NO:261、SEQ ID NO:263和SEQ ID NO:265所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列组成。

本申请的蛋白质由本申请的核酸分子编码。这些核酸分子包含编码本申请的蛋白质的核酸序列。核酸分子可以通过重组、合成或重组与合成过程相结合来产生。本申请的核酸分子可以具有野生型核酸序列或密码子修饰的核酸序列，其可以例如插入更易于被人类翻译系统识别的密码子。核酸分子也可以通过基因工程引入或去除编码不同氨基酸的密码子，例如引入编码N-连接糖基化位点的密码子。本申请的核酸分子的制备方法是本领域已知的，特别是在已知核酸序列时。核酸分子可以编码一种蛋白质或一种以上的蛋白质。

形成本申请的纳米颗粒的蛋白质可以由本申请的核酸构建体表达。如本文所用，核酸构建体是重组表达载体，即与编码蛋白质的核酸分子连接的载体，使得当将核酸构建体施用于例如受试者或器官、组织或细胞时，核酸分子可以影响蛋白质的表达。核酸构建体可以包含一个或多个核酸分子。所述载体还能够将核酸分子转运至内环境细胞，例如生物体、组织或细胞培养物。本申请的核酸构建体通过人类干预产生。核酸构建体可以是DNA、RNA或其变体。载体可以是DNA质粒、病毒载体或其他载体。载体可以是巨细胞病毒(CMV)、逆转录病毒、腺病毒、腺相关病毒、疱疹病毒、痘苗病毒、脊髓灰质炎病毒、辛德比斯病毒(Sindbis virus)或任何其他DNA或RNA病毒载体。载体可以是假型化慢病毒或逆转录病毒载体。载体可以是DNA质粒。载体可以是包含病毒成分和质粒成分的DNA质粒，以实现核酸分子的递送和表达。构建本申请的核酸构建体的方法是众所周知的。例如，参见MolecularCloning:a Laboratory Manual,3^rd edition,Sambrook et al.2001Cold Spring HarborLaboratory Press和CurrentProtocols in MolecularBiology,Ausubel et al.eds.,John Wiley&Sons,1994。载体可以是DNA质粒，例如CMV/R质粒，例如CMV/R或CMV/R 8KB(本文也称为CMV/R8kb)。CMV/R也在公开号为7,094,598的美国专利中描述。

一方面是编码本文的任何实施方案的融合蛋白的核酸分子。在一个实施方案中，所述核酸分子编码融合蛋白，所述融合蛋白包含或由与选自SEQ ID NO:245,SEQ ID NO:247,SEQ ID NO:249,SEQ ID NO:251,SEQ ID NO:253,SEQ ID NO:255,and SEQ ID NO:257的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成。

本申请的另一个实施方案是编码融合蛋白的核酸分子，所述融合蛋白包含或由与SEQ ID NO:89所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成，所述氨基酸序列与流感病毒HA蛋白的免疫原性部分连接。

在这些核酸分子编码的蛋白质中，免疫原性部分可以来自本文所详述的任何流感病毒的HA蛋白的免疫原性部分。因此，在一个实施方案中，免疫原性部分包含或由来自HA蛋白的至少6、8、10、25、50、75、100、200、300或400个连续氨基酸残基组成，所述HA蛋白包含与选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/HongKong/1/1968(1968HK,H3)、A/Michigan/45/2015(H1N1)、A/Hong Kong/4801/2014(H3N2)、A/Hong Kong/33982/2009(H9N2)、A/Singapore/1/1957(H2N2)、A/Singapore/INFIMH-16-0019/2016、A/Indonesia/5/2005(H5N1)、A/Taiwan/2/2013(H6N1)、A/Anhui/1/2013(H7N9)、A/Jiangxi-Donghu/346/2013(H10N8)、A/Michigan/45/2015(H1N1)、A/USSR/90/1977(H1N1)、A/Brevig Mission/1/1918(H1N1)、A/Brisbane/10/2007(2007Bris,H3)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008、B/Phuket/3073/2013、B/Colorado/06/2017和B/Florida/4/2006(2006Flo,B)的流感病毒的HA蛋白序列至少80％、85％、90％、91％、95％、96％、97％、98％或100％相同的氨基酸序列。

本申请的一个实施方案是编码蛋白质的核酸分子，所述蛋白质包含或由与选自SEQ ID NO:259、SEQ ID NO:261、SEQ ID NO:263、SEQ ID NO:265所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成。

本申请的另一个实施方案是编码蛋白质或融合蛋白的核酸分子，所述蛋白质或融合蛋白包含或由与选自SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、SEQ ID NO:119、SEQID NO:121、SEQ ID NO:123、SEQ ID NO:125、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137、SEQ ID NO:139、SEQ ID NO:141、SEQID NO:143、SEQ ID NO:145、SEQ ID NO:147、SEQ ID NO:149、SEQ ID NO:151、SEQ ID NO:153、SEQ ID NO:155、SEQ ID NO:157、SEQ ID NO:159、SEQ ID NO:161、SEQ ID NO:163、SEQID NO:165、SEQ ID NO:167、SEQ ID NO:169、SEQ ID NO:171、SEQ ID NO:173、SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ ID NO:181、SEQ ID NO:183、SEQ ID NO:185、SEQID NO:187、SEQ ID NO:189、SEQ ID NO:191、SEQ ID NO:193、SEQ ID NO:195、SEQ ID NO:197、SEQ ID NO:199、SEQ ID NO:201、SEQ ID NO:203、SEQ ID NO:205、SEQ ID NO:207、SEQID NO:209、SEQ ID NO:211、SEQ ID NO:213、SEQ ID NO:215、SEQ ID NO:217、SEQ ID NO:219、SEQ ID NO:221、SEQ ID NO:223、SEQ ID NO:225和SEQ ID NOS:340-346所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同的氨基酸序列组成。

本申请的另一个实施方案是包含或由氨基酸序列组成的核酸分子，所述氨基酸序列与选自SEQ ID NO:258,SEQ ID NO:260,SEQ ID NO:262,and SEQ ID NO:264所示的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同。

本申请的一个实施方案是包含或由氨基酸序列组成的核酸分子，所述氨基酸序列与选自SEQ ID NO:90、SEQ ID NO:92、SEQ ID NO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ IDNO:100、SEQ ID NO:102、SEQ ID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118、SEQ ID NO:120、SEQ IDNO:122、SEQ ID NO:124、SEQ ID NO:126、SEQ ID NO:128、SEQ ID NO:130、SEQ ID NO:132、SEQ ID NO:134、SEQ ID NO:136、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:142、SEQ IDNO:144、SEQ ID NO:146、SEQ ID NO:148、SEQ ID NO:150、SEQ ID NO:152、SEQ ID NO:154、SEQ ID NO:156、SEQ ID NO:158、SEQ ID NO:160、SEQ ID NO:162、SEQ ID NO:164、SEQ IDNO:166、SEQ ID NO:168、SEQ ID NO:170、SEQ ID NO:172、SEQ ID NO:174、SEQ ID NO:176、SEQ ID NO:178、SEQ ID NO:180、SEQ ID NO:182、SEQ ID NO:184、SEQ ID NO:186、SEQ IDNO:188、SEQ ID NO:190、SEQ ID NO:192、SEQ ID NO:194、SEQ ID NO:196、SEQ ID NO:198、SEQ ID NO:200、SEQ ID NO:202、SEQ ID NO:204、SEQ ID NO:206、SEQ ID NO:208、SEQ IDNO:210、SEQ ID NO:212、SEQ ID NO:214、SEQ ID NO:216、SEQ ID NO:218、SEQ ID NO:220、SEQ ID NO:222和SEQ ID NO:224所示的序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同，或100％相同。

本申请的一个方面是包含本申请的核酸分子的重组细胞。本申请的一个方面是包含本申请的核酸分子的重组病毒。

本申请的纳米颗粒可用于引发对流感病毒的免疫反应。免疫反应的一种类型是B细胞反应，它诱导产生针对引发免疫反应的抗原的抗体。虽然所有抗体都能够与引发产生抗体的免疫反应的抗原结合，但优选的抗体是提供针对流感病毒的广泛异源亚型保护的抗体。因此，本申请的纳米颗粒引发与来源于选自甲型流感病毒、乙型流感病毒和丙型流感病毒的病毒的流感HA蛋白结合的抗体。这些纳米颗粒可以引发与来源于选自H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15、H16、H17和H18流感病毒的HA蛋白结合的抗体。这些纳米颗粒可以引发与来源于流感病毒株的流感HA蛋白的头部和/或茎部区域结合的抗体，所述流感病毒选自A/New Caledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Singapore/1/1957(1957Sing,H2)、A/Hong Kong/1/1968(1968HK,H3)、A/Brisbane/10/2007(2007Bris,H3)、A/Indonesia/05/2005(2005Indo,H5)、B/Florida/4/2006(2006Flo,B)、A/Perth/16/2009(2009Per,H3)、A/Brisbane/59/2007(2007Bris,H1)、B/Brisbane/60/2008(2008Bris,B)及其抗原变体。

在一个实施方案中，本申请的纳米颗粒引发结合流感蛋白的抗体，所述流感蛋白包含与选自SEQ ID NO:8、11、14和17的氨基酸序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列。

由本申请的纳米颗粒诱导产生的保护性抗体可以通过影响病毒生命周期中的任何步骤来防止病毒感染。例如，保护性抗体可以防止流感病毒附着在细胞上、进入细胞、向细胞质中释放病毒核糖核蛋白、在被感染的细胞中形成新的病毒颗粒和/或新的病毒颗粒从被感染的宿主细胞膜中出芽。由本申请的纳米颗粒诱导产生的抗体优选防止流感病毒附着或进入宿主细胞，防止病毒膜与内体膜融合，或防止新形成的病毒从被感染的宿主细胞中释放。

由于流感病毒茎区的氨基酸序列是高度保守的，所以由本申请的纳米颗粒诱导产生的与茎区结合的保护性抗体可能具有广泛的保护作用，即由本申请的纳米颗粒诱导产生的保护性抗体可以预防一种以上类型、组、亚型和/或菌株的流感病毒的侵害。因此，本申请的一个实施方案是纳米颗粒，所述纳米颗粒诱导产生广泛的保护性抗体，所述抗体结合流感病毒HA蛋白的头部和/或茎部区域。这些纳米颗粒可以引发与一种以上蛋白质结合的抗体的产生，所述蛋白质包含与选自SEQ ID NO:1、4、7、10、13、16、19、22、25、28、31、34和37所示序列至少80％、85％、90％、91％、95％、96％、97％、98％或99％相同或100％相同的氨基酸序列。

本申请的一个方面是包含本文的任何纳米颗粒的疫苗组合物(疫苗)。由于本申请的纳米颗粒引发对流感病毒的免疫反应，所以这种组合物可作为疫苗以保护受试者免受流感病毒的感染。本申请的疫苗还可以包含其他成分，例如佐剂、缓冲液等。示例性的佐剂包括磷酸铝、苯扎氯铵、乌苯美司和QS21；遗传佐剂，例如IL-2基因或其片段、粒细胞巨噬细胞集落刺激因子(GM-CSF)基因或其片段、IL-18基因或其片段、趋化因子(C-C基序)配体21(CCL21)基因或其片段、IL-6基因或其片段、CpG、LPS、TLR激动剂和其他免疫刺激基因；蛋白质佐剂，例如IL-2或其片段、粒细胞巨噬细胞集落刺激因子(GM-CSF)或其片段、IL-18或其片段、趋化因子(C-C基序)配体21(CCL21)或其片段、IL-6或其片段、CpG、LPS、TLR激动剂和其他免疫刺激细胞因子或其片段；脂质佐剂，如阳离子脂质体、N3(阳离子脂质)、单磷酰基脂质A(MPL1)；其他佐剂包括霍乱毒素、肠毒素、FMS样酪氨酸激酶3配体(Flt-3L)、布比卡因、马卡因和左旋咪唑。

本申请的疫苗可以包括一种以上流感病毒类型、组、亚型或毒株的免疫原性部分。这种疫苗可以包含纳米颗粒，每个纳米颗粒包含来自一种以上流感病毒类型、组、亚型或毒株的HA蛋白的免疫原性部分。这种纳米颗粒(或疫苗)被称为镶嵌疫苗(或纳米颗粒)。镶嵌纳米颗粒(或疫苗)可包含来自尽可能多的的流感HA蛋白的免疫原性部分，以引发足以保护被施用者免受所需类型、组、亚型或毒株的病毒的免疫应答。在一个实施方案中，疫苗包含来自至少两种不同流感毒株(即二价疫苗)，或来自至少三种不同流感毒株(即三价疫苗)，或来自至少四种不同流感毒株(即四价疫苗)，或来自至少五种不同流感毒株(即五价疫苗)的HA蛋白的免疫原性部分。在一个实施方案中，疫苗包含来自至少六种不同流感毒株(六价)的HA蛋白的免疫原性部分。这种组合的一个实例是纳米颗粒，其包含来自甲型流感1型HA蛋白、甲型流感2型HA蛋白和乙型流感HA蛋白的免疫原性部分。在本申请的示例性疫苗中，纳米颗粒包含来自H1流感病毒、H2流感病毒、H3流感病毒、乙型流感病毒的HA蛋白的免疫原性部分。在本申请的另一个示例性疫苗中，纳米颗粒包含来自H1流感病毒、H3流感病毒、维多利亚系的乙型流感病毒和山形系的流感病毒的HA蛋白的免疫原性部分。

本申请的疫苗可以包含一种以上本申请的纳米颗粒，每种纳米颗粒表达来自相同类型、亚型或毒株的HA蛋白的免疫原性部分，其中不同种类的纳米颗粒表达来自不同类型、亚型的流感病毒毒株的HA蛋白的免疫原性部分。例如，疫苗可以包括表达来自H1流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自H3流感病毒的HA蛋白的免疫原性部分的纳米颗粒、表达来自第一种乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒以及表达来自第二种乙型流感病毒的HA蛋白的免疫原性部分的纳米颗粒。

本申请提供了向受试者接种流感病毒疫苗的方法，所述方法包括给受试者施用本申请的纳米颗粒或疫苗，使得受试者产生针对流感病毒的免疫应答。在这些方法中，如上所述，纳米颗粒可以是单价纳米颗粒或多价纳米颗粒。本申请的一个实施方案是一种向受试者接种流感病毒疫苗的方法，通过向受试者施用本申请的纳米颗粒以在受试者中引发针对流感病毒的免疫应答。

术语“个体”、“受试者”和“患者”在本领域中是众所周知的，并且在本申请中可互换使用，以指代任何易受流感病毒感染的人或其他动物。其实例包括但不限于人类和其他灵长类动物，包括非人灵长类动物，如黑猩猩和其他猿类和猴类；家畜，如牛、羊、猪、海豹、山羊和马；家养哺乳动物，如狗和猫；实验动物，包括啮齿动物，如小鼠、大鼠和豚鼠；鸟类，包括家禽、野生鸟类和野禽，例如鸡、火鸡和其他家禽、鸭子、鹅等。术语个人、受试者和患者本身并不表示特定的年龄、性别、种族等。因此，任何年龄的个体，无论是男性还是女性，都旨在被本申请所涵盖，并且包括但不限于老人、成人、儿童、婴儿、婴幼儿和幼儿。同样，本申请的方法可以应用于任何种族，包括例如高加索人(白人)、非裔美国人(黑人)、美洲原住民、夏威夷原住民、西班牙人、拉丁美洲人、亚洲人和欧洲人。众所周知，“被感染的受试者”体内有流感病毒。如本文所用，“接种过疫苗的受试者”已经接种了旨在提供针对流感病毒的保护作用的疫苗。

在本申请的疫苗接种方法中，被接种的受试者可能已经暴露于流感病毒。如本文所用，术语暴露(exposed)、暴露(exposure)等表示受试者已经与已知感染流感病毒的人或动物接触。本申请的疫苗可以使用本领域技术人员熟知的技术来施用。配制和给药技术可以参见例如“雷氏药学大全”第18版，1990，Mack Publishing Co.,Easton,PA。疫苗可以通过包括但不限于传统注射器、无针注射装置或微喷射轰击基因枪的方式给药。合适的给药途径包括但不限于胃肠外给药，例如肌内、皮内、皮下或髓内注射，以及鞘内、直接心室内、静脉内、腹膜内、鼻内或眼内注射。对于注射，本申请的纳米颗粒可以配制在水溶液中，优选在生理相容的缓冲液中，例如汉克斯溶液、林格氏液或生理盐水缓冲液。

本申请的疫苗或纳米颗粒可用于保护受试者免受异源流感病毒的感染。也就是说，使用来自一种流感病毒株的HA蛋白的免疫原性部分制成的疫苗能够保护受试者免受不同流感病毒株的感染。例如，可以施用本申请的疫苗，其包含来自流感A/New Caledonia/20/1999(1999NC,H1)的HA蛋白的免疫原性部分，以保护受试者免受包括但不限于A/NewCaledonia/20/1999(1999NC,H1)、A/California/04/2009(2009CA,H1)、A/Singapore/1/1957(1957Sing,H2)、A/Hong Kong/1/1968(1968HK,H3)、A/Brisbane/10/2007(2007Bris,H3)、A/Indonesia/05/2005(2005Indo,H5)、A/Perth/16/2009(2009Per,H3)和/或A/Brisbane/59/2007(2007Bris,H1)流感病毒的感染。

本申请的疫苗或纳米颗粒可用于保护受试者免受抗原性差异的流感病毒的感染。抗原性差异是指流感病毒株随着时间的推移发生变异的趋势，从而改变向免疫系统表达的氨基酸。这种随时间的突变也被称为抗原漂移。因此，例如，本申请的疫苗包含来自流感病毒的A/New Caledonia/20/1999(1999NC,H1)毒株的HA蛋白的免疫原性部分，该疫苗能够保护受试者免受早期的、抗原性差异的流感毒株以及未来进化的(或变异的)流感毒株的感染。

由于本申请的纳米颗粒表达了HA蛋白的免疫原性部分，所以它们可用于检测针对流感病毒的抗体(即抗流感抗体)。因此，本申请的一个实施方案是使用本申请的纳米颗粒检测抗流感病毒抗体的方法。抗流感抗体的检测通常可以通过将抗流感抗体存在的测试样品的至少一部分与本申请的纳米颗粒接触并检测抗体-纳米颗粒复合物的存在与否来完成。样品中存在可检测的纳米颗粒-抗体复合物则表明样品含有抗流感抗体。

在这些方法中，可以从待检测抗流感病毒抗体是否存在的受试者身上获得或收集样品。受试者可能会或不会被怀疑有抗流感抗体，或被怀疑已经暴露于流感病毒。样品是从受试者获得的任何可用于检测抗流感病毒抗体是否存在的样本，例如可用于检测抗流感病毒抗体是否存在的体液。可用于实施这些方法的体液的实例包括但不限于血液、血浆、血清、泪液和唾液。血液或血液衍生的流体，如血浆、血清等，是特别适合作为样品的体液。本领域的技术人员可以容易地识别适用于实施本申请的方法的其他的样品，并且可以使用本领域已知的方法从受试者身上收集和制备此类样品。样品可以在分析前冷藏或冷冻。

只要本申请的纳米颗粒与抗流感病毒抗体结合，本申请的任何纳米颗粒就可用于实施所公开的方法。适用的纳米颗粒及其生产方法已在本文中详细描述。

因为本申请的分析可用于检测样品中的抗流感病毒抗体，包括血液样品，所以这种分析可用于鉴定具有抗流感抗体的受试者。因此，本申请的一个方面是鉴定具有抗流感病毒抗体的受试者的方法，所述方法将来源于待检测抗流感抗体的受试者的样品与本申请的纳米颗粒接触，并分析接触的样品中抗体-纳米颗粒复合物的存在。样本中抗体-纳米颗粒复合物的存在表明受试者具有抗流感抗体。

如果样本中未检测到抗流感抗体，则该结果表明受试者没有抗流感病毒抗体。受试者在试验前可能怀疑具有或不具有抗流感病毒抗体。所公开的方法也可用于确定受试者是否已经暴露于一种或多种特定类型、组、亚型或毒株的流感病毒。为了进行这种测定，从过去某个时间(例如，大于约1年、大于约2年、大于约3年、大于约4年、大于约5年等)对一种或多种特定类型、组、亚型或毒株的流感病毒抗体检测呈阴性(即，缺乏抗体)的受试者获得样品。然后使用本申请的基于纳米颗粒的测定法，检测样品中是否存在针对一种或多种流感病毒类型、组、亚型或毒株的抗流感病毒抗体。如果检测表明存在此类抗体，且受试者在之前的检测中被鉴定为抗流感抗体阴性，则该受试者被鉴定为暴露于一种或多种流感病毒类型、组、亚型或毒株。因此，本申请的一个方面是鉴定已经暴露于特定类型、组、亚型或毒株的流感病毒的受试者的方法，所述方法通过将来自正被检测抗流感抗体的受试者的样品的至少一部分与本申请的纳米颗粒接触，并分析接触的样品中抗体-纳米颗粒复合物的存在与否或水平来实现。在此步骤检测抗体-纳米颗粒复合物的存在与否或水平表明近期抗流感抗体的存在或水平。近期的抗流感抗体水平可以与过去的抗流感抗体水平进行比较，并且如果受试者在确定过去的抗流感抗体水平之后近期的抗流感抗体水平相对于过去的抗流感抗体水平增加，则表明受试者已经暴露于流感病毒。

本申请的方法也可用于确定受试者对流感疫苗的反应。因此，本申请的一个方面是用于检测受试者对流感疫苗的反应的方法，所述方法通过给受试者施用流感病毒疫苗，将来自受试者的样品的至少一部分与本申请的纳米颗粒接触，并分析接触的样品中抗体/纳米颗粒复合物的存在与否或水平，其中抗体-纳米颗粒复合物的存在与否或水平指示近期抗流感抗体的存在与否或水平。在这些方法中，样品中抗体水平的增加超过了受试者接种疫苗前的抗体水平，表明疫苗在受试者中诱导了免疫反应。

向受试者施用的流感疫苗可以但不是必须包含本申请的疫苗，只要纳米颗粒包含能够结合由施用的疫苗诱导的抗流感抗体的HA蛋白。施用流感疫苗的方法是本领域技术人员已知的。

在任何一种所述分析方法中，可以使用任何已知的分析形式对来自受试者的样品进行分析，以检测抗体-纳米颗粒复合物的存在与否或水平。在一个实施方案中，使用选自径向扩散系统测定法、酶联免疫测定法、竞争性酶联免疫测定法、放射免疫测定法、荧光免疫测定法、化学发光测定法、侧向流动测定法、流通测定法、基于颗粒的结合测定(例如，使用磁性颗粒或塑料聚合物等颗粒，如乳胶或聚苯乙烯珠)、免疫沉淀测定法、BioCoreJ测定法(例如，使用胶体金)、免疫斑点测定法(例如，CMG＝s Immunodot系统,Fribourg,Switzerland)和免疫印迹测定法(例如，蛋白印迹法)、磷光测定法、流通测定法、色谱法、基于聚丙烯酰胺凝胶电泳(PAGE)的测定法、表面等离子共振测定法、生物膜层干涉测定法、分光光度法和电子传感器测定法。

在一个实施方案中，所述方法包括在施用疫苗之前确认受试者中抗流感抗体水平的步骤。当然，如果以前的医疗记录可用，也可以从此类信息中确定受试者体内的抗流感抗体水平。

本申请还包括制备和/或使用本申请的纳米颗粒的试剂盒。所述试剂盒可以包括例如本申请的蛋白质、编码本申请的蛋白质的本申请的核酸分子、和/或适于检测抗流感抗体或向受试者接种疫苗的纳米颗粒和疫苗。合适的检测手段包括本文中利用本申请的纳米颗粒的技术。试剂盒还可以包含可检测的标记物，例如选择性结合纳米颗粒的抗体或其他指示分子。所述试剂盒还可以包含例如试剂盒中成分的使用说明、缓冲液、标签、容器、管道、小瓶、注射器等相关的成分。

另一方面，本申请提供了包含氨基酸序列的多肽，所述氨基酸序列与选自SEQ IDNO:43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85和87的氨基酸序列的全长至少80％、85％、90％、91％、95％、96％、97％、98％、99％或100％相同。本申请这一方面的非天然存在的多肽可用于例如制备纳米颗粒，包括但不限于表达本文所述的HA抗原的纳米颗粒。这方面的多肽在哺乳动物细胞中的表达显著优于本文所述的SEQ IDNO:41的相关第一蛋白质。这些多肽中的每一种都可以用于例如与本文所述的第二蛋白质形成纳米结构。

表3确定了每个多肽中被鉴定为存在于所组装的纳米结构界面的残基数量(即：“被鉴定的界面残基”)。可以看出，如SEQ ID NO:43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85和87所示的示例性多肽的界面残基的数量在4-13的范围内。在各种实施方案中，本发明的分离的多肽包含与选自SEQ ID NO:43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85和87所示的多肽的氨基酸序列的全长至少80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％相同的氨基酸序列，并且所述氨基酸序列至少在1、2、3、4、5、6、7、8、9、10、11、12或13个确定的界面位置上相同(取决于给定多肽的界面残基的数量)。在其他实施方案中，本发明的分离的多肽包含的氨基酸序列在其全长上与选自45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85和87的多肽的氨基酸序列至少80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％相同，并且至少20％、25％、33％、40％、50％、60％、70％、75％、80％、90％或100％与鉴定的界面位置相同。在其他实施方案中，相对于参考序列的可变性可以包括表4中指出的表面残基，和/或本文所述的保守氨基酸取代。

这些多肽中的任一种都可以用来与例如本文所述的第二蛋白质形成纳米颗粒。因此，在另一个实施方案中，本申请提供了一种纳米颗粒，包括：

(a)多个第一组件，每个第一组件包含多个相同的第一多肽,所述第一多肽包含与选自SEQ ID NO:43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85和87所示的多肽的氨基酸序列的全长至少80％、85％、90％、91％、95％、96％、97％、98％、99％或100％相同的氨基酸序列，其中所述多肽与SEQ ID NO:41所示的氨基酸序列全长不同；和

(b)多个第二组件，每个第二组件包含多个第二多肽；

其中第一多肽的序列不同于第二多肽的序列；并且

其中多个第一组件与多个第二组件非共价相互作用以形成纳米颗粒。

本申请这一方面的纳米颗粒可用于任何合适的目的，包括但不限于递送载体，由于纳米颗粒可包封感兴趣的分子和/或可以修饰第一和/或第二多肽以结合目的分子(诊断、治疗、用于成像和其他应用的可检测分子等)。这方面的纳米颗粒非常适于包括疫苗设计、靶向递送治疗药物和生物能源的各种应用。在一个实施方案中，第一组件和第二组件中的一个或两个包含至少一种第一多肽和/或至少一种第二多肽，从而形成融合蛋白，其中所述多肽与抗原的免疫原性部分结合；其中纳米颗粒在其表面表达抗原的免疫原性部分。可以使用任何合适的多肽抗原，包括但不限于病原体特异性抗原，例如来自肝炎(甲、乙、丙、戊等)的病毒、人乳头瘤病毒、单纯疱疹病毒、巨细胞病毒、Epstein-Barr病毒、流感病毒、副流感病毒、冠状病毒、鼻病毒、肠道病毒、麻疹病毒、流行性腮腺炎病毒、脊髓灰质炎病毒、狂犬病病毒、人类免疫缺陷病毒、呼吸道合胞病毒、轮状病毒、风疹病毒、水痘带状疱疹病毒、埃博拉病毒、巨细胞病毒、马尔堡病毒、诺如病毒、天花病毒、任何黄病毒(包括但不限于西尼罗河病毒、黄热病病毒、登革热病毒、蜱传脑炎病毒、寨卡病毒和日本脑炎病毒)、炭疽杆菌、百日咳杆菌、沙眼衣原体、破伤风梭菌、艰难梭菌(Clastridium difficile)、白喉棒状杆菌、贝纳柯克斯体、大肠埃希菌、流感嗜血杆菌、幽门螺杆菌、杜氏利什曼原虫、热带利什曼原虫和巴西利什曼原虫(L.tropica andL.braziliensis)、结核分枝杆菌、麻风分枝杆菌、脑膜炎奈瑟菌、恶性疟原虫、卵形疟原虫、三日疟原虫和间日疟原虫、铜绿假单胞菌、伤寒沙门氏菌、血血吸虫、曼氏血吸虫、肺炎链球菌(A组和B组)、金黄色葡萄球菌、弓形虫、布氏锥虫、克氏弧菌和霍乱弧菌；肿瘤/肿瘤细胞内或表面表达的抗原(包括但不限于p53(结直肠癌)、甲胎蛋白(生殖细胞肿瘤；肝细胞癌)、癌胚抗原(肠癌)、CA-125(卵巢癌)、人表皮生长因子受体-2(HER-2、乳腺癌)、MUC-1(乳腺癌)、NY-ESO-1(食管癌、非小细胞肺癌)、上皮肿瘤抗原(乳腺癌)、酪氨酸酶(恶性黑色素瘤)、二唾液酸神经节苷脂(GD2、神经母细胞瘤)、黑色素瘤相关抗原基因-1(MAGE-1，恶性黑色素瘤)和β淀粉样蛋白(用于阿尔茨海默病和其他淀粉样蛋白基疾病)等。

在本申请的这一方面的纳米颗粒的各种实施方案中，所述多个第二多肽与SEQ IDNO:89(I53-dn5B)所示的氨基酸序列的全长至少80％、85％、90％、91％、95％、96％、97％、98％、99％或100％相同。同一性百分比或第二蛋白质不包括在产生融合蛋白(例如，如下文和本文所述的融合到抗原上的第二蛋白质)时可能添加的任何额外的结构域。

以上针对表达HA抗原的纳米颗粒的所有实施方案和讨论同样适用于本申请这一方面的纳米颗粒。因此，在一个实施方案中，第一多肽和第二多肽的长度可以在30至250个氨基酸之间，30至225个氨基酸之间，30至200个氨基酸之间，30至175个氨基酸之间，50至250个氨基酸之间，50至225个氨基酸之间，50至200个氨基酸之间，50至175个氨基酸之间，75至250个氨基酸之间，75至225个氨基酸之间，75-200个氨基酸之间，75-175个氨基酸之间，100-250个氨基酸之间，100-225个氨基酸之间，100-200个氨基酸之间，100-175个氨基酸之间，125-250个氨基酸之间，125-225个氨基酸之间，125-200个氨基酸之间，125-175个氨基酸之间，150至250个氨基酸之间，150至225个氨基酸之间，150至200个氨基酸之间，150至175个氨基酸之间。类似地，该方面的纳米颗粒可以具有适合预期用途的任何形状和/或对称性，包括但不限于四面体、八面体、二十面体、十二面体及其截断形式。这方面的纳米颗粒可以是任何适于预期用途的尺寸，包括但不限于约10nm至约100nm的直径。

在其他方面，本申请提供了编码本申请多肽的核酸、包含这些核酸分子的表达载体以及包含这些核酸分子和/或表达载体的细胞。如上所述的核酸分子、表达载体和细胞的所有实施方案同样适用于本申请的这一方面。

为了清楚而在本申请上下文的独立的方面中描述的某些特征，也可以在实施例中单独或组合提供。相反，为了简洁而在本申请上下文的一个方面描述的多个特征，也可以单独提供或者以任何合适的子组合提供。本申请具体包含本申请的所有方面和实施方案的所有组合，并且于本文中公开，就好像每个和每个组合都被单独且明确地公开一样。此外，本申请具体包含所有子组合，并且于本文中公开，就像每个这样的子组合在本文中被单独且明确地公开一样。

本文所讨论的出版物仅用于在本申请的申请日之前公开。本文中的任何内容都不应被解释为承认本申请内容无权凭借先前的发明而早于这些出版物。此外，所提供的出版日期可能与实际出版日期不同，这可能需要独立确认。本文提及的所有出版物都通过引用并入本文，以公开和描述与引用这些出版物中的方法和/或材料。

实施例

实施例1

纳米颗粒的体外组装

A.包含HA蛋白和三聚体组件结构域的三聚体结构单元的表达和筛选

包括HA融合体在内的三聚体结构单元的人类密码子优化序列是由Genscript合成的，或者使用Gibson组件从现有的DNA克隆而来。所有基因都在具有HTLV-1R区的CMV I/E启动子下被克隆到哺乳动物表达载体pVRC 8400中，利用XbaI和AvrII限制性位点进行高效转录。根据生产商的说明书，通过用Expifectamine^TM(赛默飞世尔科技)瞬时转染Expi293F^TM细胞来表达重组蛋白。转染后4天通过离心收集细胞，上清液0.22μm过滤。首先通过SDS-PAGE选择用于表达的最佳构建体，并通过生物层干涉法检测抗原性。三聚体的形成通过尺寸排阻色谱法评估，随后通过差示扫描荧光分析法评估热稳定性。

B.五聚体结构单元的表达和筛选

所有在细菌中表达的基因均通过Genscript、IntegratedDNATechnologies合成并克隆到表达质粒中，或者使用Gibson组件从现有的DNA中克隆而来。所有基因都被克隆到NdeI和XhoI限制性位点之间的pET29b(+)载体上。重组蛋白通过IPTG诱导在T7表达感受态细胞(T7 Express CompetentE.coli)(New England Biolabs)中表达，并在18℃过夜表达。细胞裂解后，通过裂解物上清液进行SDS-PAGE检测可溶性蛋白表达。

将所有来源于人类细胞系的表达基因克隆到来源于具有N-末端附加IgK信号肽的现有DNA的pCMV载体中，修饰的基因被插入到NcoI和XhoI限制位点之间。用聚乙烯亚胺(PEI)瞬时转染Expi293F^TM细胞以表达重组蛋白。使用抗His辣根过氧化物酶(Anti-HisHRP)标记抗体(R&D系统)，通过蛋白质印迹法针对遗传融合的六组氨酸标签评估蛋白质表达。

C.包含HA蛋白和三聚体组件结构域的三聚体结构单元的表达和纯化

使用生产商的方案，通过瞬时转染Expi293F^TM细胞(赛默飞世尔科技)来表达重组蛋白。转染后4天通过离心收集细胞，上清液0.45μm过滤。将氯化钠和pH 8.0的Tris-HCl分别添加到350mM和50mM，上清液与镍Sepharose^TMExcel树脂(通用电气医疗集团)一起孵育过夜。树脂用含30mM咪唑的缓冲液洗涤两次，然后用300mM咪唑洗脱五次。洗脱的蛋白质在pH7.4的磷酸盐缓冲液(PBS)(赛默飞世尔科技)中，加载到Superdex^TM 200Increase10/300或HiLoad Superdex^TM 200pg柱(通用电气医疗集团)上，通过尺寸排阻色谱法进一步纯化。纯化的蛋白质通过紫外/可见吸收光谱定量，并在液氮中快速冷冻并于-80℃储存。

D.五聚体结构单元的表达和纯化

重组蛋白通过IPTG诱导在T7表达感受态细胞(T7Express CompetentE.coli)中表达，并在18℃过夜表达。细胞裂解后，裂解物通过离心分离，0.22μm过滤，然后上样到HisTrapTM HP柱(通用电气医疗集团)，并在洗脱前用0.75％CHAPS洗涤以去除内毒素。用500mM咪唑洗脱之前，使用0-150mM咪唑梯度洗脱以进一步去除杂质。洗脱的蛋白质在pH8.0的25mM Tris-HCl、150mM NaCl、5％甘油中加载到Superdex^TM 200Increase 10/300或HiLoad Superdex^TM 75pg柱上，通过尺寸排阻色谱法进一步纯化目的蛋白质。通过紫外/可见吸收光谱对纯化的蛋白质进行定量，并使用Charles River Endosafe^TM PTS进行测试以确保低内毒素，然后在液氮中快速冷冻并于-80℃储存。

E.HA纳米颗粒的体外组装

通常，100％价态颗粒(每个二十面体纳米颗粒20个相同的HA三聚体)是通过将含HA的三聚体蛋白质和五聚体结构单元等摩尔浓度混合来制备(在图1A中示例性描述)。

对于“A/Michigan/45/2015(H1N1)HA 1-676Y98F no_lkr_dn5B.SA”(缩写为“H1-dn5B”)与100％HA三聚体价态的“I53-dn5 A”的组装，在每种蛋白质以150mM的NaCl作为基础盐的情况下，将两种蛋白质混合至最终浓度为25.0μM。用移液管将溶液充分混合，并在室温下静置30分钟。组装的纳米颗粒样品在含有pH 8.0的25mM Tris-HCl、150mM NaCl和5％甘油的缓冲液中，加载到Superose^TM 6Increase 10/300柱(通用电气医疗集团)上纯化。该方法对于“A/Hong_Kong/4801/2014(H3N2)HA 1-676Y98F GG lkr dn5B.SA”(缩写为“H3-dn5 B”)与具有100％三聚体价态的“I53-dn5 A”的组装是相同的，除混合浓度为22.0μM以外。该方法还用于将等摩尔量的“I53-dn5 A”与“A/Anhui/1/2013(H7N9)HA 1-676Y98Fnolkr dn5B.SA”(缩写为“H7-dn5 B”)、“A/Singapore/1/1957(H2N2)SS rpk22676no_lkr_dn5B.SA”(缩写为“ssH2-dn5 B”)、“A/Indonesia/5/2005(H5N1)delF SS rpk22676no_lkr_dn5B.SA”(缩写为“ssH5-dn5 B”)、“A/Anhui/1/2013(H7N9)SS no256676no lkr dn5B.SA”(缩写为“ssH7-dn5 B”)或“A/Jiangxi-Donghu/346/2013(H10N8)SS no256676no lkrdn5B.SA”(缩写为“ssH10-dn5 B”)分别混合来组装具有100％HA三聚体的另外的纳米颗粒。每种蛋白质的最终浓度范围为23.1-38.9μM。

对于“B/Brisbane/60/2008HA 1-674no lkr dn5B.SA”(缩写为“Bvic-dn5B”)与“I53-dn5A”的组装，将两种蛋白质混合至最终浓度为18.7μM，每种蛋白质的基础盐浓度为1M的NaCl。为了实现这一点，在添加“I53-dn5 A”之前，首先将“Bvic-dn5 B”与25mM Tris-HCl(pH8.0)、1.85M的NaCl和5％甘油混合，使得最终盐浓度为1M的NaCl。用移液管将溶液充分混合，并在室温下静置30分钟。组装的纳米颗粒样品在含有pH 8.0的25mM Tris-HCl、1MNaCl、5％甘油的缓冲液中，加载到Superose^TM6 Increase 10/300柱(通用电气医疗集团)上纯化。该方案还用于组装100％HA三聚体纳米颗粒，方法是将等摩尔量的“I53-dn5 A”与“A/Indonesia/5/2005(H5N1)1-676delF Y98F no lkr dn5B.SA”(缩写为“H5-dn5 B”)、“A/Taiwan/2/2013(H6N1)HA 1-676Y98F no lkr dn5B.SA”(缩写为“H6-dn5 B”)或“A/Jiangxi-Donghu/346/2013(H10N8)HA 1-676Y98F no lkr dn5B.SA”(缩写为“H10-dn5 B”)混合，每种蛋白质的最终浓度范围为16.7-22.0μM。

对于“B/Phuket/3073/2013HA 1-674no lkr dn5B.SA”(缩写为“Byam-dn5B”)与“I53-dn5A”的组装，将两种蛋白质混合至最终浓度为13.0μM。为此，在加入“I53-dn5 A”之前，首先将“Byam-dn5 B”与pH 8.0的25mM Tris-HCl、3.85M NaCl和5％甘油混合，使得最终盐浓度为2M NaCl。用移液管将溶液充分混合，并在室温下静置30分钟。组装好的纳米颗粒样品在含有pH 8.0的25mM Tris-HCl、2M NaCl、5％甘油的缓冲液中，加载到Superose^TM6Increase 10/300柱(通用电气医疗集团)上纯化。

对于“H1-dn5B”、“H3-dn5 B”、“Bvic-dn5 B”和“Byam-dn5 B”与“I53-dn5 A”的共组装，首先在浓盐溶液中以等摩尔浓度混合HA三聚体，然后与“I53-dn5 A”混合，使得组装期间NaCl的最终浓度为1M。这是通过添加25mM Tris-HCl(pH8.0)、1.85M的NaCl、5％甘油来进行的，以使所有四种HA三聚体的初始浓度分别为5.25μM，或所有四种蛋白质之间的总浓度为21.0μM。然后加入“I53-dn5 A”，使其浓度与HA三聚体的摩尔浓度之和相等，最终浓度为“I53-dn5a”15.75μM，“H1-dn5 B”、“H3-dn5 B”、“Bvic-dn5 B”和“Byam-dn5 B”分别为3.94μM。用移液管将溶液充分混合，并在室温下静置30分钟。组装的纳米颗粒样品在含有pH8.0的25mM Tris-HCl、1M的NaCl、5％甘油的缓冲液中，加载到Superose^TM 6Increase 10/300柱(通用电气医疗集团)上纯化。该方案已被用于类似地不同的数量和种类HA三聚体的各种组合的共同组装，包括“H1-dn5 B”、H3-dn5 B”、“H5-dn5B”、“H6-dn5 B”、“H7-dn5 B”、“H10-dn5 B”、“Bvic-dn5 B”和“Byam-dn5 B”的部分或全部。对于每一种情况，该方案已被修改为混合不同浓度HA三聚体，然后与“I53-dn5A”混合，“I53-dn5 A”的量与总的HA三聚体摩尔浓度的总和相等，“I53-dn5 A”与包含的HA三聚体的所有摩尔浓度的总和的最终浓度范围分别为15.9-26.4μM。基于相同的原理，该方案进一步推广到“ssH2-dn5 B”、“ssH5-dn5b”、“ssH7-dn5b”和/或“ssH10-dn5 B”的各种组合，仅通过不添加额外的浓盐溶液将基础NaCl浓度保持在150mM。

对于所有组件，在存在和不存在还原剂的情况下，通过SDS-PAGE分析样品负载和峰值馏分，以确认每个峰值馏分中蛋白质的种类。将含HA的纳米颗粒的峰值馏分合并，并在0.22μM下过滤，以去除聚集体或杂质。最终物质通过紫外/可见吸收光谱进行定量，在液氮中快速冷冻并于-80℃储存。

图1B-1至1B-4中分别示出了以“H1-dn5B”在I53-dn5纳米颗粒上表达作为本申请纳米颗粒制备过程中进行的表达、纯化、组装和质量控制监测的一个实例。尺寸排阻色谱法(SEC)用于从未组装的蛋白质组分中纯化组装的纳米颗粒(图1B-1)。SEC洗脱峰的SDS-PAGE用于通过确认“H1-dn5 B”和“I53-dn5 A”蛋白质构建体的存在与否来鉴定蛋白质的种类(图1B-2)。紫外/可见光谱用于定量蛋白质，并通过比较快速冷冻和解冻前后的光谱来确认冻融过程中的最小蛋白质聚集(图1B-3)。负染色透射电子显微镜用于确认冻融过程后纳米颗粒的完整性(图1B-4)。

以“H1-dn5B”、“H3-dn5 B”、“Bvic-dn5 B”和“Byam-dn5b”的HA蛋白构建体共组装在I53-dn5纳米颗粒上作为另一个实例，图1C-1至1C-4中分别示出了本申请的纳米颗粒制备过程中进行的表达、纯化、组装和质量控制监测。尺寸排阻色谱法(SEC)用于从未组装的蛋白质组分中纯化组装的纳米颗粒(图1C-1)。SEC洗脱峰的SDS-PAGE用于通过确认“H1-dn5B”、“H3-dn5 B”、“Bvic-dn5 B”、“Byam-dn5b”和“I53-dn5”蛋白质构建体的存在与否来鉴定蛋白质的种类(图1C-2)。紫外/可见光谱用于定量蛋白质，并通过比较快速冷冻和解冻前后的光谱来确认冻融过程中的最小蛋白质聚集(图1C-3)。负染色透射电子显微镜用于确认冻融过程后纳米颗粒的完整性(图1C-4)。

F.热稳定性检测

使用Prometheus NT.48instrument(诺坦普科技)通过纳米差示扫描荧光测定法(nanoDSF)检测纯化蛋白质的热诱导去折叠。用PBS将HA-三聚体结构单元融合体和组装材料稀释至大约0.5mg/mL，并在温度增量设置为1℃/min的条件下从30℃～95℃进行nanoDSF检测。热转变中点温度(Tm)由热解折叠曲线获得，所述曲线以斜率(Δ(F350 nm/F330nm)/ΔT)与350nm和330nm荧光检测产生的温度(℃)为对应关系绘制。nanoDSF结果如下表所示：

G.紫外吸收光谱分析

蛋白质含量的定量应用NanoDrop^TM2000紫外/可见光谱仪、NanoDrop^TM 8000紫外/可见光谱仪(赛默飞世尔科技)或Cary 8454紫外/可见光谱仪(安捷伦科技公司)完成。使用Expasy Protparam^TM网络服务器基于氨基酸序列计算蛋白质样品的消光系数和分子量，并使用比尔定律计算蛋白质浓度。

H.含HA纳米颗粒的电镜观察

使用基于Tris的缓冲液将样品稀释至0.05-0.075mg/mL制备负染色EM样品，将6.0μL样品在辉光放电铜碳涂层格栅上孵育1分钟，然后将格栅快速浸入40μL的水滴中。在几秒钟内用Whatman 1号滤纸将水吸干，立即将格栅浸入6.0μL的染色液(2％w/v甲酸铀酰)中。迅速吸走污渍，几秒钟内将格栅浸入另一个6.0μL的染色液中，染色液留在格栅上30秒钟。结束后，吸干污渍风干5分钟后成像。在配备有Gatan US4000 CCD相机的FEI TecnaiSpirit^TM透射电子显微镜上记录图像，使用Leginon软件以52000倍的标称放大率在-1um和-4um之间的散焦范围内进行数据收集。使用DoG^TMPicker拾取粒子。使用RELION^TM 2.1提取和分析粒子图像。RELION^TM 2.1生成2D类平均值，使用EMAN2^TM图像处理器根据RELION^TM 2.1类平均值生成旋转平均图像。

I.通过生物膜层干涉测量法(BLI)进行的抗原表征

通过预包被的抗人Fc抗体，将各种HA头部和茎部定向的单克隆抗体固定在AHC生物传感器(fortéBio)上。所有生物传感器在使用前都在PBS中水合。使用的单克隆抗体包括：针对“H1-dn5 B”构建变体的CR6261(抗组1茎区)、CR8020(抗组2茎区)、MEDI8552(跨组抗茎区)、CT149(跨组抗茎区)、F005-126(抗H3头部)、5J8(抗H1头部)、2D1(抗H1头部)和441D6(抗H1头部)；针对所有其他构建体的CR6261、CR8020、MEDI8552、CT149、CR9114(广谱流感A/B抗茎区)、C05(选择性抗受体结合位点)和F045-092(大部分抗H3受体结合位点)。将单克隆抗体加载到AHC生物传感器上(0.030mg/mL，持续5分钟)后，将传感器短暂浸入检测缓冲液(1％BSA-PBS)中。然后将生物传感器浸入含有用检测缓冲液(0.030mg/mL)稀释的各种HA-dn5 B构建体的孔中5分钟。所有检测步骤均在30℃下进行，并在Octet HTX仪器(fortéBio)中以1000rpm的速度搅拌。使用Octet分析软件(版本9.0)进行数据分析。

实施例2

免疫用HA纳米颗粒的制备

如上所述，鸡尾酒纳米颗粒疫苗包含表达单一或多个不同HA抗原的纳米颗粒。“镶嵌”纳米粒子疫苗包括共表达多种不同HA抗原的纳米颗粒。在免疫接种之前，以含HA的鸡尾酒纳米颗粒和含HA的镶嵌纳米颗粒与50mM Tris-HCl、2MNaCl缓冲液和Addavax^TM佐剂分别为1∶1的等摩尔量比例制备含HA的鸡尾酒纳米颗粒和含HA的镶嵌纳米颗粒，其的最终浓度如下表所示：

M*小鼠F*雪貂P*灵长类

实施例3

动物免疫和测试

A.动物实验

从Jackson实验室获得6至8周龄的雌性BALB/cJ小鼠用于免疫原性研究。从TripleF农场获得6月龄的雪貂用于免疫原性研究。其他研究中应用的五至六岁的恒河猴被再次用于免疫原性研究。非人灵长类动物根据性别、体重、年龄和研究前的既往研究记录随机分组。

B.免疫接种

小鼠(N＝10)在第0周、第4周、第8周或第24周用6μg市售四价流感疫苗(QIV，2017年)、市售三价流感疫苗(TIV，2011年)或含或不含Addavax^TM(Invivogen)佐剂(50％v/v)的HA纳米颗粒的各种制剂进行肌内注射免疫(IM)三次。针对每只小鼠制备体积为100μL的免疫原，将50μL肌内注射注入每只小鼠后腿。免疫前后定期收集血清样品，用于免疫分析。对于实验性病毒攻毒研究，小鼠鼻内接种10-20倍LD₅₀的甲A/Vietnam/1203/04H5N1或小鼠适应的A/Philippines/2/82H3N2病毒。所有的攻毒研究都是在Bioqual公司的ABSL-2或ABSL-3实验室中进行的。在感染后的14天里，每天两次监测小鼠临床症状的发展，并每天称重。任何比攻击前体重减轻20％或更多的动物都会被人道安乐死。

雪貂(N＝6)在第0周和第4周用20μg市售QIV 2017或含Addavax^TM(50％v/v)佐剂的HA纳米颗粒进行两次免疫。针对每只雪貂制备体积为500μL的免疫原，并通过肌内注射注入四肢。免疫前后定期收集血清样品，用于免疫分析。

恒河猴(N＝4)在第0周和第8周用60μg市售QIV 2017或含Addavax^TM(50％v/v)佐剂的HA纳米颗粒进行三次免疫。针对每只非人灵长类动物(NHP)制备体积为1.0mL的免疫原，并通过肌内注射注入四肢。免疫前后定期收集血清样品，用于免疫分析。

C.酶联免疫吸附试验(ELISA)

ELISA检测免疫血清中HA特异性免疫球蛋白G(IgG)的水平。将MaxiSorp^TM板(Nunc)用含重组HA或仅含茎HA蛋白(0.1mL/孔，2μg/mL)的PBS溶液包被4℃过夜。然后用含5％脱脂牛奶的PBS在37℃下封闭酶标板一小时。用含0.05％吐温20的PBS洗涤后，加入4倍系列稀释的免疫血清(0.1mL/孔)，酶标板在37℃孵育1小时。在另一次洗涤后，加入辣根过氧化物酶(HRP)标记二抗，并在37℃孵育1小时。然后通过加入KPL SureBlueTM TMB过氧化物酶底物(seracare)使酶标板显影，并通过加入1M H₂SO₄终止反应。通过SpectraMax^TM Paradigm酶标仪(Molecular Devices)在450nm处(背景扣除650nm)下读取酶标板吸光度。使用Prism^TM(GraphPad)分析减去背景的数据，并根据与阈值(空白孔的平均吸光度+2s.d或0.1，以最高者为准)的四参数非线性回归曲线拟合计算终点滴度。

D.基于报告的微中和试验

所有病毒均采用反向遗传学技术制备，含有从圣裘德儿童研究医院(St.JudeChildren'sResearch Hospital)获得的双顺反子polI/polII启动子控制编码每个甲型流感病毒片段的8个质粒。对于报告的病毒，编码PB1片段的质粒被修饰以表达荧光报告基因(tdKatushka2或mKate2)并用于病毒拯救。在TPCK处理的胰蛋白酶(1μg/mL，Sigma)存在下，所有报告病毒在组成型表达病毒PB1(MDCK-SIAT1-PB1)的工程MDCK-SIAT1细胞中繁殖，并在MDCK-SIAT1-PB1细胞中滴定。使用OptiMEM^TM-TPCK(含1μg/mL TPCK-胰蛋白酶和青霉素/链霉素的OptiMEM^TM)对RDE处理的血清或抗体进行四倍系列稀释，并将预先滴定的流感病毒稀释至最终浓度为每孔1000～4000个荧光焦点。在96孔板中，在加入底物MDCK-PB1细胞之前，将血清或抗体与病毒混合并在37℃孵育1小时。每个平板上包括单独的病毒(VC)对照孔和单独的稀释剂(CC)对照孔。在测定前24小时，以每孔1.0×10⁴个细胞接种，并在使用前用PBS洗涤一次。然后将50μL抗体/病毒混合物一式两份加入预洗涤细胞的孔中，并将平板在37℃和5％CO₂加湿气氛中孵育18～22小时。使用Celigo^TM图像血细胞计数器(NexcelomBiosciences)自动计数每个孔中的单个荧光细胞。通过将VC对照限制为0％和将CC对照限制为100％来计算中和百分比，并针对血清/抗体浓度绘图。在Prism(GraphPad)软件中，通过四参数非线性拟合模型生成曲线拟合。分别从每个血清样品或抗体的曲线拟合中得出半最大抑制浓度(IC₅₀)。

E.假型病毒中和试验

制备了表达流感病毒HA和HA的基于慢病毒的假病毒。简而言之，假病毒是通过转染HA和相应NA的293T细胞以及慢病毒包装和报告质粒产生的。编码人II型跨膜丝氨酸蛋白酶TMPRSS2基因的质粒也被共转染用于HA的有效蛋白水解活化。转染后48小时，收集上清液，过滤，冷冻直至使用。免疫血清用受体破坏酶(RDE(II)，SEIKEN Accurate Chemicaland Scientific)处理，并在进行中和试验前热灭活。将系列稀释的RDE处理的血清或单克隆抗体与预滴定的HA-NA假型病毒(A/Vietnam/1203/04H5N1和A/Anhui/1/13H7N9)共同孵育，然后加入到预接种有293A细胞的96孔板。在37℃、5％CO₂加湿气氛下孵育过夜后，更换培养基并将平板再孵育2天。通过裂解细胞和添加底物(Promega)来检测萤火虫荧光素酶活性。通过使用SpectraMax^TM L(分子装置)测量发光。如上所述进行数据分析并计算IC₅₀。

F.统计分析

除非另有说明，多组比较是通过非参数Kruskal-Wallis检验和Dunn事后分析在Prism^TM(GraphPad)软件中进行。当P值小于0.05时，被认为差异显著。统计方法和P值范围见附图说明。

G.免疫测试

为了比较市售HA疫苗、本申请的纳米颗粒构建体混合物和本申请的镶嵌纳米颗粒构建体的免疫效力，用不同剂量的上述疫苗组合物免疫小鼠，并通过ELISA和对病毒的血清微中和(MN)滴度来评估病毒滴度。在用6μg的Addavax^TM佐剂的免疫原(图2A)或6μg无Addavax^TM佐剂的免疫原(图2B)进行第三次免疫后2周(第10周)，通过ELISA检测HA的血清抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒在含或不含Addavax^TM佐剂的小鼠中均具有免疫原性，并对所有四种疫苗匹配的病毒都引发了相似的或更好的HA结合抗体反应。

类似地，在用0.6μg(图2C)或0.06μg(图2D)的Addavax^TM佐剂免疫原进行第三次免疫后2周(第10周)，通过ELISA检测HA的血清抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒在含或不含Addavax^TM佐剂的小鼠中均具有免疫原性，并对所有四种疫苗匹配的病毒产生相似的或更好的HA结合抗体反应。

图3A和3B示出了用6μgAddavax^TM佐剂免疫原(图3A)或6μg无佐剂免疫原(图3B)进行第三次免疫后2周(第10周)，通过基于报告的MN测定，检测小鼠中同源病毒的病毒中和抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒都能引发针对与疫苗匹配的H1N1、H3N2和乙型流感病毒相似的或更好的病毒中和抗体反应。

也比较了用6μg市售QIV 2017、鸡尾酒纳米颗粒或镶嵌纳米颗粒免疫的小鼠中对同源病毒的血凝抑制(HAI)抗体滴度。图4示出了用含有Addavax^TM佐剂的免疫原进行第三次免疫后2周(第10周)，通过使用火鸡红细胞检测血清HAI抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒均能针对与疫苗相匹配的甲型H1N1流感病毒和乙型两个谱系的流感病毒产生相似地或更好的的血凝抑制抗体反应。

还检测了用6μg市售QIV 2017、鸡尾酒纳米颗粒或含有Addavax^TM佐剂的镶嵌纳米颗粒免疫的小鼠对异源亚型病毒HA的抗体反应。在第三次免疫后2周(第10周)检测血清抗体滴度。图5A示出了通过ELISA检测的异亚型HA的血清抗体滴度。图5B示出了通过假型病毒中和试验检测的针对H5N1或H7N9假病毒的中和抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒均可引发针对H5N1、H6N1、H7N9和H10N8病毒的显著性更高的异源亚型HA结合抗体应答。在鸡尾酒纳米颗粒和镶嵌纳米颗粒免疫的小鼠中也检测到对异源亚型H5N1和H7N9假病毒的血清中和活性，但在用市售疫苗免疫的动物中未检出。

用6μg市售QIV 2017、鸡尾酒纳米颗粒或镶嵌纳米颗粒进行第三次免疫后的10～14周(第18～22周)的小鼠用疫苗不匹配的H3N2和异源亚型H5N1病毒进行攻毒试验。实验性病毒感染后的各存活曲线绘制于Kaplan-Meier曲线图上。图6A示出了先前用含有Addavax^TM佐剂的免疫原(左)或不含佐剂的免疫原(右)免疫并用疫苗不匹配的H3N2攻击的小鼠的存活曲线。图6B示出了先前用含有Addavax^TM佐剂的免疫原(左)或不含佐剂的免疫原(右)免疫并用异源亚型H5N1病毒攻击的小鼠的存活曲线。含有Addavax^TM的鸡尾酒纳米颗粒和镶嵌纳米颗粒对用疫苗不匹配的H3N2和异源亚型H5N1病毒进行攻击实验的免疫小鼠均提供了完全保护，而市售疫苗仅分别提供了部分保护或没有引发针对H3N2或H5N1的保护。尽管针对疫苗不匹配的H3N2感染的保护效力降低，但在没有佐剂的情况下，鸡尾酒纳米颗粒和镶嵌纳米颗粒都为免疫小鼠提供了针对H5N1感染的完全保护，而市售疫苗在没有佐剂的情况下对H3N2或H5N1攻击都没有表现出保护作用。

通过ELISA定期检测用6μg市售QIV 2017、鸡尾酒纳米颗粒或含Addavax^TM佐剂的镶嵌纳米颗粒免疫后小鼠的血清中同源病毒的HA结合抗体滴度。在第0、4和24周进行免疫接种。图7显示，与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒在两次免疫接种后都引发了对疫苗匹配病毒HA的更高和更持久的抗体反应。尽管总的抗体滴度随着时间的推移而下降，但第三次免疫后将所有HA的滴度都提高到了峰值。

同样，用6μg市售QIV 2017、鸡尾酒纳米颗粒或含Addavax^TM佐剂的镶嵌纳米颗粒免疫的小鼠中，通过ELISA检测与抗原错配和异源亚型病毒HA的HA结合抗体滴度。在第三次免疫后2周(第26周)检测组1HA(图8A)和组2HA(图8B)的血清抗体滴度。鸡尾酒纳米颗粒和镶嵌纳米颗粒在第26周(即本研究第三次增强后2周)引发的对疫苗不匹配病毒和异源亚型病毒HA的抗体反应均显著高于市售疫苗。

用6μg市售QIV 2017、鸡尾酒纳米颗粒或含Addavax^TM佐剂的镶嵌纳米颗粒进行免疫的小鼠对HA茎区的抗体反应。图9示出了在第三次免疫后2周(第26周)通过ELISA检测第1组仅HA茎的血清抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒均对HA的恒定茎区(最广泛中和抗体的靶点)产生更显著的抗体反应。

检测用60μg市售QIV 2017、鸡尾酒纳米颗粒或含Addavax^TM佐剂的镶嵌纳米颗粒免疫的恒河猴中针对同源病毒HA的HA结合抗体滴度。图10示出了在第一次免疫后4周(第4周)和第二次免疫后2周和4周(分别为第10周和第12周)通过ELISA检测的同源HA的血清抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和含Addavax^TM佐剂的在非人灵长类动物中都具有免疫原性，并且即使在第一次给药后，也能引发针对所有四种疫苗匹配的病毒的HA结合抗体反应。还检测了这些小鼠中对同源病毒的血清中和抗体滴度。图11示出了在免疫前(第0周)、第一次免疫后4周(第4周)和第二次免疫后2周(第10周)通过基于报告的微中和(MN)分析检测同源病毒的血清中和抗体滴度。与市售疫苗相比，即使在第一剂之后，鸡尾酒纳米颗粒和镶嵌纳米颗粒都引发了对疫苗匹配的H1N1、H3N2和乙型流感病毒的病毒中和抗体反应。还检测了这些恒河猴中针对同源病毒的HAI抗体滴度。图12示出了在第二次免疫接种后2周(第10周)使用火鸡红细胞检测的针对同源病毒的血清HAI抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒都能引起与疫苗匹配的H1N1和乙型流感2种病毒系病毒的HAI抗体反应。在第二次免疫后2周(第10周)，还通过ELISA检测了这些恒河猴对异源亚型HA的血清抗体滴度。图13显示，与市售疫苗相比，鸡尾酒纳米颗粒和镶嵌纳米颗粒在两次给药后均引发了针对H5N1、H6N1、H7N9和H10N8病毒HA的异亚型HA结合抗体反应。

还用20μg市售QIV 2017、本申请的纳米颗粒混合物或本申请的含Addavax^TM佐剂的镶嵌纳米颗粒免疫雪貂。图14示出了在第二次免疫后2周(第6周)通过ELISA检测的同源HA的血清抗体滴度。与市售疫苗相比，鸡尾酒纳米颗粒和含Addavax^TM佐剂的镶嵌纳米颗粒对雪貂均具有免疫原性，并对所有四种疫苗匹配的病毒都引发类似的HA结合抗体反应。图15示出了在这些雪貂第二次免疫后2周(第6周)通过ELISA检测的血清中异源亚型HA的HA结合抗体滴度。与市售疫苗相比，镶嵌纳米颗粒在两次给药后引发了可检测的针对H5N1和H7N9病毒的异源亚型HA结合抗体反应。

在用6μg多亚类型鸡尾酒纳米颗粒(即包含来自不同HA亚型的多种HA抗原的鸡尾酒纳米颗粒)或含Addavax^TM佐剂的镶嵌纳米颗粒免疫的小鼠中，检测针对同源病毒HA的HA结合抗体滴度。8X鸡尾酒纳米颗粒由八个独立的纳米颗粒组成，每个纳米颗粒在其表面表达独特的HA。8X镶嵌纳米颗粒在每个颗粒上表达相同的一组八个不同的HA。3-3-2镶嵌纳米颗粒由三个独立的颗粒组成，每个颗粒在其表面表达三种不同的第1组HA、三种不同的第2组HA或两种不同的乙型流感病毒HA。图16示出了在用Addavax^TM佐剂免疫原进行第三次免疫后2周(第10周)通过ELISA检测的不同HA的血清抗体滴度。多特异性鸡尾酒纳米颗粒和镶嵌纳米颗粒引发了针对所有八种疫苗匹配病毒的强HA结合抗体反应。在这些用6μg多亚类型鸡尾酒纳米颗粒或者含Addavax^TM佐剂的镶嵌纳米颗粒免疫的小鼠中，对HA茎区的抗体反应进行检测。图17示出了在第三次免疫后2周(第10周)通过ELISA检测的第1组仅HA茎(左)和第2组仅HA茎(右)的血清抗体滴度。对于第1组和第2组病毒而言，多特异性鸡尾酒纳米颗粒和镶嵌纳米颗粒引发了对HA恒定茎区的抗体反应，HA恒定茎区是最广泛中和抗体的靶点。

在用6μg市售TIV 2011、茎鸡尾酒纳米颗粒或含Addavax^TM佐剂的茎镶嵌纳米颗粒免疫的小鼠中，检测同源和异源亚型病毒HA的HA结合抗体滴度。茎鸡尾酒纳米颗粒由四个独立的纳米颗粒组成，每个纳米颗粒表面表达独特的仅茎区的HA(两个组1和两个组2仅HA茎)。茎镶嵌纳米颗粒在每个颗粒上表达相同的一组四种不同的仅茎HA。图18示出了在用含Addavax^TM佐剂免疫原进行第三次免疫后2周(第10周)通过ELISA检测的HA的血清抗体滴度。茎鸡尾酒纳米颗粒和镶嵌纳米颗粒都在第1组和第2组的多个亚型HA中引发了显著的HA结合抗体反应，这与市售疫苗不同，后者主要引发亚型特异性抗体反应。在用含Addavax^TM佐剂免疫原进行第三次免疫后2周(第10周)，通过ELISA在这些小鼠中检测针对乙型流感病毒HA的HA结合抗体滴度。图19示出了茎鸡尾酒纳米颗粒和镶嵌纳米颗粒都引发了对高度分化的乙型流感病毒的可检测的HA结合抗体反应，尽管免疫原是基于甲型流感病毒的茎设计的。

图20示出了SEC图谱，其表明与原始的I53-dn5A(图20A)相比，I53-dn5 A.1(LF4_W16G)的表达增加(图20B)，这是通过去除半胱氨酸和增加其他突变提供的。这两种蛋白质均在1L的大肠杆菌培养物中表达，并在SEC检测前在Superdex^TM 75Increase 10/300柱上通过镍亲和层析纯化。

图21示出了从哺乳动物HEK293F细胞培养物中分泌的I53-dn5A.2(LF2_W16G_LS)五聚体组分的纯化和组装。在通过镍亲和层析从细胞上清液中表达和纯化后，在Superdex^TM200Increase10/300柱(通用电气医疗集团)上分泌的I53-dn5 A.2五聚体组分的SEC显示了正确的大小(图21A)。将SEC纯化的I53-dn5 A.2与I53-dn5 B三聚体混合，成功组装了纳米颗粒，并在Superose^TM 6Increase 10/300柱(通用电气医疗集团)上通过SEC验证(图21B)。通过SDS-PAGE确认了所有步骤中的蛋白质(图21C)。

虽然已经描述了某些示例性实施方案，但是这些实施方案仅通过示例的方式呈现，并且不旨在限制本文公开的发明的范围。因此，前面描述中的任何内容都不意味着任何特定的特征、特性或步骤是必要的或不可缺少的。本文描述的新方法和组合物可以以各种其他形式呈现；此外，在不脱离本文所公开的本发明的精神的情况下，可以对本文描述的方法和系统的形式进行各种省略、替换和改变。所附权利要求及其等同物旨在涵盖落在本文公开的发明范围和精神内的各种形式或修改。

序列表

<110> 美国政府（由卫生和人类服务部的部长所代表）

华盛顿大学

兼清 优

巴尼·S·格雷厄姆

赛汉·博约格鲁·巴纳姆

丽贝卡·A·吉莱斯皮

尼尔·P·王

丹尼尔 ·埃利斯

王京阳

<120> 基于纳米颗粒的流感病毒疫苗及其用途

<130> 19-163-PCT

<150> US 62/803,218

<151> 2019-02-08

<160> 349

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Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu

515

<210> 5

<211> 234

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 5

Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn Ile

1 5 10 15

Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala

20 25 30

Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly Thr

35 40 45

Cys Phe Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu

50 55 60

Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser

65 70 75 80

Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys Pro

85 90 95

His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys

100 105 110

Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys

115 120 125

Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr Thr

130 135 140

Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val

145 150 155 160

Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg

165 170 175

Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu

180 185 190

Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu Val

195 200 205

Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly Ile

210 215 220

Ile Ile Ser Asp Thr Pro Val His Asp Cys

225 230

<210> 6

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 6

Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala Val

1 5 10 15

Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys

20 25 30

Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu

35 40 45

Leu Val Leu Leu Glu

50

<210> 7

<211> 517

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 7

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln

515

<210> 8

<211> 226

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 8

Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile

1 5 10 15

Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys

20 25 30

Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn Cys Tyr Pro

35 40 45

Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser

50 55 60

Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr Gly Val Thr

65 70 75 80

Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser Ser Ser Phe

85 90 95

Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr Tyr Pro Ala

100 105 110

Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile

115 120 125

Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile Phe Leu Tyr

130 135 140

Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln

145 150 155 160

Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro

165 170 175

Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu

180 185 190

Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys

195 200 205

Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly

210 215 220

Lys Cys

225

<210> 9

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 9

Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile

1 5 10 15

Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys

20 25 30

Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu

35 40 45

Leu Val Ala Leu Glu

50

<210> 10

<211> 539

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 10

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala

530 535

<210> 11

<211> 242

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 11

Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg

1 5 10 15

Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His

20 25 30

Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg

35 40 45

Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Ile

50 55 60

Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn Ala Pro Gly Gly

65 70 75 80

Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly

85 90 95

Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asn Asp Lys

100 105 110

Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr Ile Cys

115 120 125

Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn

130 135 140

Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe

145 150 155 160

Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly

165 170 175

Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg

180 185 190

Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile

195 200 205

Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser

210 215 220

Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala

225 230 235 240

Asp Cys

<210> 12

<211> 56

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 12

Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg

1 5 10 15

Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp

20 25 30

Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu

35 40 45

Leu Ala Val Leu Leu Ser Asn Glu

50 55

<210> 13

<211> 538

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 13

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr

50 55 60

Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys

130 135 140

Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro

180 185 190

Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His

195 200 205

Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro

210 215 220

Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser

225 230 235 240

Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln

245 250 255

Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr

260 265 270

Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp

275 280 285

Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile

290 295 300

Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser

305 310 315 320

Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro

325 330 335

Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg

340 345 350

Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala

355 360 365

Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly

370 375 380

Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys

385 390 395 400

Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu

405 410 415

Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp

420 425 430

Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu

435 440 445

Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser

450 455 460

Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu

465 470 475 480

Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn

485 490 495

Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg

500 505 510

Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe

515 520 525

Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala

530 535

<210> 14

<211> 241

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 14

Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg

1 5 10 15

Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala Ser Ile Leu His

20 25 30

Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg

35 40 45

Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu Lys Ile

50 55 60

Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys Ala Pro Gly Gly

65 70 75 80

Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn Ala Thr Ser Lys

85 90 95

Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro Lys Asp Asn Tyr

100 105 110

Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro Tyr Ile Cys Thr

115 120 125

Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Lys

130 135 140

Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro Gln Lys Phe Thr

145 150 155 160

Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Asp

165 170 175

Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile

180 185 190

Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr Gly Thr Ile Val

195 200 205

Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly

210 215 220

Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp

225 230 235 240

Cys

<210> 15

<211> 56

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 15

Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg

1 5 10 15

Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp

20 25 30

Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu

35 40 45

Leu Ala Val Leu Leu Ser Asn Glu

50 55

<210> 16

<211> 518

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 16

Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Thr Ala Val Arg Gly Asp

1 5 10 15

Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp

20 25 30

Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu

35 40 45

Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro

50 55 60

Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro

65 70 75 80

Glu Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu

85 90 95

Lys Glu Asn Pro Arg Asp Gly Leu Cys Phe Pro Gly Ser Phe Asn Asp

100 105 110

Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys

115 120 125

Val Lys Ile Leu Pro Lys Asp Arg Trp Thr Gln His Thr Thr Thr Gly

130 135 140

Gly Ser Arg Ala Cys Ala Val Ser Gly Asn Pro Ser Phe Phe Arg Asn

145 150 155 160

Met Val Trp Leu Thr Glu Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly

165 170 175

Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val

180 185 190

His His Pro Asn Asp Glu Lys Glu Gln Arg Thr Leu Tyr Gln Asn Val

195 200 205

Gly Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Lys Arg Ser Thr

210 215 220

Pro Asp Ile Ala Thr Arg Pro Lys Val Asn Gly Leu Gly Ser Arg Met

225 230 235 240

Glu Phe Ser Trp Thr Leu Leu Asp Met Trp Asp Thr Ile Asn Phe Glu

245 250 255

Ser Thr Gly Asn Leu Ile Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys

260 265 270

Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys

275 280 285

Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro

290 295 300

Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val

305 310 315 320

Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val Pro Gln

325 330 335

Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly

340 345 350

Gly Trp Gln Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His Ser Asn

355 360 365

Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala

370 375 380

Phe Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn

385 390 395 400

Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg

405 410 415

Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp

420 425 430

Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu

435 440 445

Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Met

450 455 460

Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe

465 470 475 480

Tyr His Lys Cys Asp Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr

485 490 495

Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Asn Arg Asn Glu

500 505 510

Ile Lys Gly Val Ser Ala

515

<210> 17

<211> 232

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 17

Cys Lys Leu Asn Gly Ile Pro Pro Leu Glu Leu Gly Asp Cys Ser Ile

1 5 10 15

Ala Gly Trp Leu Leu Gly Asn Pro Glu Cys Asp Arg Leu Leu Ser Val

20 25 30

Pro Glu Trp Ser Tyr Ile Met Glu Lys Glu Asn Pro Arg Asp Gly Leu

35 40 45

Cys Phe Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu

50 55 60

Ser Ser Val Lys His Phe Glu Lys Val Lys Ile Leu Pro Lys Asp Arg

65 70 75 80

Trp Thr Gln His Thr Thr Thr Gly Gly Ser Arg Ala Cys Ala Val Ser

85 90 95

Gly Asn Pro Ser Phe Phe Arg Asn Met Val Trp Leu Thr Glu Lys Gly

100 105 110

Ser Asn Tyr Pro Val Ala Lys Gly Ser Tyr Asn Asn Thr Ser Gly Glu

115 120 125

Gln Met Leu Ile Ile Trp Gly Val His His Pro Asn Asp Glu Lys Glu

130 135 140

Gln Arg Thr Leu Tyr Gln Asn Val Gly Thr Tyr Val Ser Val Gly Thr

145 150 155 160

Ser Thr Leu Asn Lys Arg Ser Thr Pro Asp Ile Ala Thr Arg Pro Lys

165 170 175

Val Asn Gly Leu Gly Ser Arg Met Glu Phe Ser Trp Thr Leu Leu Asp

180 185 190

Met Trp Asp Thr Ile Asn Phe Glu Ser Thr Gly Asn Leu Ile Ala Pro

195 200 205

Glu Tyr Gly Phe Lys Ile Ser Lys Arg Gly Ser Ser Gly Ile Met Lys

210 215 220

Thr Glu Gly Thr Leu Glu Asn Cys

225 230

<210> 18

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 18

Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Glu Ala Val

1 5 10 15

Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg Leu Glu Asn Leu Asn Lys

20 25 30

Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu

35 40 45

Leu Val Leu Met Glu

50

<210> 19

<211> 520

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 19

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser

1 5 10 15

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val

20 25 30

Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile

35 40 45

Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys

50 55 60

Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn

65 70 75 80

Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val

85 90 95

Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Phe Pro Gly Ser Phe Asn

100 105 110

Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu

115 120 125

Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser

130 135 140

Ser Gly Val Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe

145 150 155 160

Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile

165 170 175

Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp

180 185 190

Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln

195 200 205

Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg

210 215 220

Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly

225 230 235 240

Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn

245 250 255

Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile

260 265 270

Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly

275 280 285

Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser

290 295 300

Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys

305 310 315 320

Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser

325 330 335

Pro Gln Arg Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile

340 345 350

Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His

355 360 365

Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln

370 375 380

Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys

385 390 395 400

Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu

405 410 415

Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp

420 425 430

Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg

435 440 445

Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val

450 455 460

Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe

465 470 475 480

Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn

485 490 495

Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg

500 505 510

Glu Glu Ile Ser Gly Val Ser Ala

515 520

<210> 20

<211> 233

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 20

Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val

1 5 10 15

Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val

20 25 30

Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Thr Asn Asp Leu

35 40 45

Cys Phe Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu

50 55 60

Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser Ser

65 70 75 80

Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr

85 90 95

Leu Gly Ser Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys

100 105 110

Asn Ser Thr Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn Gln

115 120 125

Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala Ala

130 135 140

Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Ile Gly

145 150 155 160

Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser

165 170 175

Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile Leu

180 185 190

Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala

195 200 205

Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile Met

210 215 220

Lys Ser Glu Leu Glu Tyr Gly Asn Cys

225 230

<210> 21

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 21

Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val

1 5 10 15

Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys

20 25 30

Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu

35 40 45

Leu Val Leu Met Glu

50

<210> 22

<211> 523

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 22

Met Ile Ala Ile Val Val Ile Ala Ile Leu Ala Ser Ala Gly Lys Ser

1 5 10 15

Asp Lys Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Thr Gln Val

20 25 30

Asp Thr Leu Leu Glu Lys Asn Val Thr Val Thr His Ser Val Glu Leu

35 40 45

Leu Glu Asn Gln Lys Glu Lys Arg Phe Cys Lys Ile Met Asn Lys Ala

50 55 60

Pro Leu Asp Leu Lys Asp Cys Thr Ile Glu Gly Trp Ile Leu Gly Asn

65 70 75 80

Pro Lys Cys Asp Leu Leu Leu Gly Asp Gln Ser Trp Ser Tyr Ile Val

85 90 95

Glu Arg Pro Asn Ala Gln Asn Gly Ile Cys Phe Pro Gly Val Leu Asn

100 105 110

Glu Leu Glu Glu Leu Lys Ala Phe Ile Gly Ser Gly Glu Arg Val Glu

115 120 125

Arg Phe Glu Met Phe Pro Lys Ser Thr Trp Ala Gly Val Asp Thr Ser

130 135 140

Arg Gly Val Thr Asn Ala Cys Pro Ser Tyr Thr Ile Asp Ser Ser Phe

145 150 155 160

Tyr Arg Asn Leu Val Trp Ile Val Lys Thr Asp Ser Ala Thr Tyr Pro

165 170 175

Val Ile Lys Gly Thr Tyr Asn Asn Thr Gly Thr Gln Pro Ile Leu Tyr

180 185 190

Phe Trp Gly Val His His Pro Leu Asp Thr Thr Val Gln Asp Asn Leu

195 200 205

Tyr Gly Ser Gly Asp Lys Tyr Val Arg Met Gly Thr Glu Ser Met Asn

210 215 220

Phe Ala Lys Ser Pro Glu Ile Ala Ala Arg Pro Ala Val Asn Gly Gln

225 230 235 240

Arg Ser Arg Ile Asp Tyr Tyr Trp Ser Val Leu Arg Pro Gly Glu Thr

245 250 255

Leu Asn Val Glu Ser Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Tyr

260 265 270

Lys Phe Val Ser Thr Asn Lys Lys Gly Ala Val Phe Lys Ser Asp Leu

275 280 285

Pro Ile Glu Asn Cys Asp Ala Thr Cys Gln Thr Ile Thr Gly Val Leu

290 295 300

Arg Thr Asn Lys Thr Phe Gln Asn Val Ser Pro Leu Trp Ile Gly Glu

305 310 315 320

Cys Pro Lys Tyr Val Lys Ser Glu Ser Leu Arg Leu Ala Thr Gly Leu

325 330 335

Arg Asn Val Pro Gln Ile Ala Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly

355 360 365

Tyr His His Glu Asn Ser Gln Gly Ser Gly Tyr Ala Ala Asp Arg Glu

370 375 380

Ser Thr Gln Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Ile

385 390 395 400

Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val Asp His Glu Phe Ser

405 410 415

Asn Leu Glu Arg Arg Ile Gly Asn Leu Asn Lys Arg Met Glu Asp Gly

420 425 430

Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu

435 440 445

Asn Glu Arg Thr Leu Asp Leu His Asp Ala Asn Val Lys Asn Leu Tyr

450 455 460

Glu Lys Val Lys Ser Gln Leu Arg Asp Asn Ala Asn Asp Leu Gly Asn

465 470 475 480

Gly Cys Phe Glu Phe Trp His Lys Cys Asp Asn Glu Cys Met Glu Ser

485 490 495

Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Gln Lys Glu Ser Lys

500 505 510

Leu Asn Arg Gln Gly Ile Glu Ser Val Ser Ala

515 520

<210> 23

<211> 236

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 23

Cys Lys Ile Met Asn Lys Ala Pro Leu Asp Leu Lys Asp Cys Thr Ile

1 5 10 15

Glu Gly Trp Ile Leu Gly Asn Pro Lys Cys Asp Leu Leu Leu Gly Asp

20 25 30

Gln Ser Trp Ser Tyr Ile Val Glu Arg Pro Asn Ala Gln Asn Gly Ile

35 40 45

Cys Phe Pro Gly Val Leu Asn Glu Leu Glu Glu Leu Lys Ala Phe Ile

50 55 60

Gly Ser Gly Glu Arg Val Glu Arg Phe Glu Met Phe Pro Lys Ser Thr

65 70 75 80

Trp Ala Gly Val Asp Thr Ser Arg Gly Val Thr Asn Ala Cys Pro Ser

85 90 95

Tyr Thr Ile Asp Ser Ser Phe Tyr Arg Asn Leu Val Trp Ile Val Lys

100 105 110

Thr Asp Ser Ala Thr Tyr Pro Val Ile Lys Gly Thr Tyr Asn Asn Thr

115 120 125

Gly Thr Gln Pro Ile Leu Tyr Phe Trp Gly Val His His Pro Leu Asp

130 135 140

Thr Thr Val Gln Asp Asn Leu Tyr Gly Ser Gly Asp Lys Tyr Val Arg

145 150 155 160

Met Gly Thr Glu Ser Met Asn Phe Ala Lys Ser Pro Glu Ile Ala Ala

165 170 175

Arg Pro Ala Val Asn Gly Gln Arg Ser Arg Ile Asp Tyr Tyr Trp Ser

180 185 190

Val Leu Arg Pro Gly Glu Thr Leu Asn Val Glu Ser Asn Gly Asn Leu

195 200 205

Ile Ala Pro Trp Tyr Ala Tyr Lys Phe Val Ser Thr Asn Lys Lys Gly

210 215 220

Ala Val Phe Lys Ser Asp Leu Pro Ile Glu Asn Cys

225 230 235

<210> 24

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 24

Lys Val Asn Ser Ile Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val

1 5 10 15

Asp His Glu Phe Ser Asn Leu Glu Arg Arg Ile Gly Asn Leu Asn Lys

20 25 30

Arg Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu

35 40 45

Leu Val Leu Leu Glu

50

<210> 25

<211> 517

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 25

Met Asn Thr Gln Ile Leu Val Phe Ala Leu Ile Ala Ile Ile Pro Thr

1 5 10 15

Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr

20 25 30

Lys Val Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr

35 40 45

Glu Thr Val Glu Arg Thr Asn Ile Pro Arg Ile Cys Ser Lys Gly Lys

50 55 60

Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly

65 70 75 80

Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile

85 90 95

Glu Arg Arg Glu Gly Ser Asp Val Cys Phe Pro Gly Lys Phe Val Asn

100 105 110

Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys

115 120 125

Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr

130 135 140

Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp

145 150 155 160

Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser

165 170 175

Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu Ile Val Trp Gly Ile His

180 185 190

His Ser Val Ser Thr Ala Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn

195 200 205

Lys Leu Val Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Val Pro

210 215 220

Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Leu Ser Gly Arg Ile Asp

225 230 235 240

Phe His Trp Leu Met Leu Asn Pro Asn Asp Thr Val Thr Phe Ser Phe

245 250 255

Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys

260 265 270

Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly

275 280 285

Asp Cys Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln

290 295 300

Asn Ile Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln

305 310 315 320

Arg Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro

325 330 335

Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly

340 345 350

Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala

355 360 365

Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile

370 375 380

Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln

385 390 395 400

Gln Phe Glu Leu Ile Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile

405 410 415

Gly Asn Val Ile Asn Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser

420 425 430

Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp

435 440 445

Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg Val Lys Arg Gln

450 455 460

Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe

465 470 475 480

His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr

485 490 495

Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile

500 505 510

Asp Pro Val Ser Ala

515

<210> 26

<211> 227

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 26

Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu

1 5 10 15

Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser

20 25 30

Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Phe Pro

35 40 45

Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser

50 55 60

Gly Gly Ile Asp Lys Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile Arg

65 70 75 80

Thr Asn Gly Ala Thr Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr

85 90 95

Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe Pro

100 105 110

Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu Ile

115 120 125

Val Trp Gly Ile His His Ser Val Ser Thr Ala Glu Gln Thr Lys Leu

130 135 140

Tyr Gly Ser Gly Asn Lys Leu Val Thr Val Gly Ser Ser Asn Tyr Gln

145 150 155 160

Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Leu

165 170 175

Ser Gly Arg Ile Asp Phe His Trp Leu Met Leu Asn Pro Asn Asp Thr

180 185 190

Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser

195 200 205

Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp

210 215 220

Ala Asn Cys

225

<210> 27

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 27

Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln Gln Phe Glu Leu Ile

1 5 10 15

Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile Gly Asn Val Ile Asn

20 25 30

Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser Tyr Asn Ala Glu Leu

35 40 45

Leu Val Ala Met Glu

50

<210> 28

<211> 516

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 28

Met Glu Thr Ile Ser Leu Met Thr Ile Leu Leu Ile Thr Thr Ala Ser

1 5 10 15

Asn Ala Asp Lys Ile Cys Ile Gly His Gln Ser Thr Asn Ser Thr Glu

20 25 30

Thr Val Asp Thr Leu Thr Glu Thr Asn Val Pro Val Thr His Ala Lys

35 40 45

Glu Leu Leu Gln Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn Leu

50 55 60

Gly His Pro Leu Ile Leu Asp Thr Cys Thr Ile Glu Gly Leu Ile Tyr

65 70 75 80

Gly Asn Pro Ser Cys Asp Leu Leu Leu Glu Gly Arg Glu Trp Ser Tyr

85 90 95

Ile Val Glu Arg Pro Ser Ala Val Asn Gly Thr Cys Phe Pro Gly Asn

100 105 110

Val Glu Asn Leu Glu Glu Leu Arg Thr Leu Phe Ser Ser Ala Ser Ser

115 120 125

Tyr Gln Arg Ile Gln Ile Phe Pro Asp Thr Thr Trp Asn Val Thr Tyr

130 135 140

Thr Gly Thr Ser Arg Ser Cys Ser Gly Ser Phe Tyr Arg Ser Met Arg

145 150 155 160

Trp Leu Thr Gln Lys Ser Gly Phe Tyr Pro Val Gln Asp Ala Lys Tyr

165 170 175

Thr Asn Asn Arg Gly Lys Asn Ile Leu Phe Val Trp Gly Ile His His

180 185 190

Pro His Thr Tyr Thr Asp Gln Thr Thr Leu Tyr Ile Arg Asn Asp Thr

195 200 205

Thr Thr Ser Val Thr Thr Glu Glu Leu Asn Arg Ile Phe Lys Pro Val

210 215 220

Ile Val Pro Arg Leu Leu Val Asn Gly Gln Gln Gly Arg Ile Asp Tyr

225 230 235 240

Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Val Arg Ser Asn

245 250 255

Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Val Leu Ser Gly Gly Ser

260 265 270

His Gly Arg Ile Leu Lys Thr Asp Leu Lys Ser Gly Asn Cys Val Val

275 280 285

Gln Cys Gln Thr Glu Lys Gly Gly Leu Asn Ser Thr Leu Pro Phe His

290 295 300

Asn Ile Ser Lys Tyr Ala Phe Gly Thr Cys Pro Lys Tyr Val Lys Val

305 310 315 320

Asn Ser Leu Lys Leu Ala Ile Gly Leu Arg Asn Val Pro Ala Arg Ser

325 330 335

Asn Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp

340 345 350

Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser Asn Asp Gln

355 360 365

Gly Val Gly Met Ala Ala Asp Arg Asp Ser Thr Gln Lys Ala Val Asp

370 375 380

Lys Ile Thr Ser Lys Val Asn Asn Ile Val Asp Lys Met Asn Lys Gln

385 390 395 400

Tyr Glu Ile Ile Asp His Glu Phe Ser Glu Val Glu Thr Arg Leu Asn

405 410 415

Met Ile Asn Asn Lys Ile Asp Asp Gln Ile Gln Asp Val Trp Ala Tyr

420 425 430

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Gln Lys Thr Leu Asp Glu

435 440 445

His Asp Ala Asn Val Asn Asn Leu Tyr Asn Lys Val Lys Arg Ala Leu

450 455 460

Gly Ser Asn Ala Met Glu Asp Gly Lys Gly Cys Phe Glu Leu Tyr His

465 470 475 480

Lys Cys Asp Asp Gln Cys Met Glu Thr Ile Arg Asn Gly Thr Tyr Asp

485 490 495

Arg Arg Lys Tyr Arg Glu Glu Ser Arg Leu Glu Arg Gln Arg Ile Glu

500 505 510

Gly Val Ser Ala

515

<210> 29

<211> 227

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 29

Cys Ala Thr Asn Leu Gly His Pro Leu Ile Leu Asp Thr Cys Thr Ile

1 5 10 15

Glu Gly Leu Ile Tyr Gly Asn Pro Ser Cys Asp Leu Leu Leu Glu Gly

20 25 30

Arg Glu Trp Ser Tyr Ile Val Glu Arg Pro Ser Ala Val Asn Gly Thr

35 40 45

Cys Phe Pro Gly Asn Val Glu Asn Leu Glu Glu Leu Arg Thr Leu Phe

50 55 60

Ser Ser Ala Ser Ser Tyr Gln Arg Ile Gln Ile Phe Pro Asp Thr Thr

65 70 75 80

Trp Asn Val Thr Tyr Thr Gly Thr Ser Arg Ser Cys Ser Gly Ser Phe

85 90 95

Tyr Arg Ser Met Arg Trp Leu Thr Gln Lys Ser Gly Phe Tyr Pro Val

100 105 110

Gln Asp Ala Lys Tyr Thr Asn Asn Arg Gly Lys Asn Ile Leu Phe Val

115 120 125

Trp Gly Ile His His Pro His Thr Tyr Thr Asp Gln Thr Thr Leu Tyr

130 135 140

Ile Arg Asn Asp Thr Thr Thr Ser Val Thr Thr Glu Glu Leu Asn Arg

145 150 155 160

Ile Phe Lys Pro Val Ile Val Pro Arg Leu Leu Val Asn Gly Gln Gln

165 170 175

Gly Arg Ile Asp Tyr Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu

180 185 190

Arg Val Arg Ser Asn Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Val

195 200 205

Leu Ser Gly Gly Ser His Gly Arg Ile Leu Lys Thr Asp Leu Lys Ser

210 215 220

Gly Asn Cys

225

<210> 30

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 30

Lys Val Asn Asn Ile Val Asp Lys Met Asn Lys Gln Tyr Glu Ile Ile

1 5 10 15

Asp His Glu Phe Ser Glu Val Glu Thr Arg Leu Asn Met Ile Asn Asn

20 25 30

Lys Ile Asp Asp Gln Ile Gln Asp Val Trp Ala Tyr Asn Ala Glu Leu

35 40 45

Leu Val Leu Leu Glu

50

<210> 31

<211> 518

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 31

Met Tyr Lys Ile Val Val Ile Ile Ala Leu Leu Gly Ala Val Lys Gly

1 5 10 15

Leu Asp Lys Ile Cys Leu Gly His His Ala Val Ala Asn Gly Thr Ile

20 25 30

Val Lys Thr Leu Thr Asn Glu Gln Glu Glu Val Thr Asn Ala Thr Glu

35 40 45

Thr Val Glu Ser Thr Gly Ile Asn Arg Leu Cys Met Lys Gly Arg Lys

50 55 60

His Lys Asp Leu Gly Asn Cys His Pro Ile Gly Met Leu Ile Gly Thr

65 70 75 80

Pro Ala Cys Asp Leu His Leu Thr Gly Met Trp Asp Thr Leu Ile Glu

85 90 95

Arg Glu Asn Ala Ile Ala Tyr Cys Phe Pro Gly Ala Thr Val Asn Val

100 105 110

Glu Ala Leu Arg Gln Lys Ile Met Glu Ser Gly Gly Ile Asn Lys Ile

115 120 125

Ser Thr Gly Phe Thr Tyr Gly Ser Ser Ile Asn Ser Ala Gly Thr Thr

130 135 140

Arg Ala Cys Met Arg Asn Gly Gly Asn Ser Phe Tyr Ala Glu Leu Lys

145 150 155 160

Trp Leu Val Ser Lys Ser Lys Gly Gln Asn Phe Pro Gln Thr Thr Asn

165 170 175

Thr Tyr Arg Asn Thr Asp Thr Ala Glu His Leu Ile Met Trp Gly Ile

180 185 190

His His Pro Ser Ser Thr Gln Glu Lys Asn Asp Leu Tyr Gly Thr Gln

195 200 205

Ser Leu Ser Ile Ser Val Gly Ser Ser Thr Tyr Arg Asn Asn Phe Val

210 215 220

Pro Val Val Gly Ala Arg Pro Gln Val Asn Gly Gln Ser Gly Arg Ile

225 230 235 240

Asp Phe His Trp Thr Leu Val Gln Pro Gly Asp Asn Ile Thr Phe Ser

245 250 255

His Asn Gly Gly Leu Ile Ala Pro Ser Arg Val Ser Lys Leu Ile Gly

260 265 270

Arg Gly Leu Gly Ile Gln Ser Asp Ala Pro Ile Asp Asn Asn Cys Glu

275 280 285

Ser Lys Cys Phe Trp Arg Gly Gly Ser Ile Asn Thr Arg Leu Pro Phe

290 295 300

Gln Asn Leu Ser Pro Arg Thr Val Gly Gln Cys Pro Lys Tyr Val Asn

305 310 315 320

Arg Arg Ser Leu Met Leu Ala Thr Gly Met Arg Asn Val Pro Glu Leu

325 330 335

Ile Gln Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Leu Glu Asn

340 345 350

Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn

355 360 365

Ala Gln Gly Thr Gly Gln Ala Ala Asp Tyr Lys Ser Thr Gln Ala Ala

370 375 380

Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Val Glu Lys Thr Asn

385 390 395 400

Thr Glu Phe Glu Ser Ile Glu Ser Glu Phe Ser Glu Ile Glu His Gln

405 410 415

Ile Gly Asn Val Ile Asn Trp Thr Lys Asp Ser Ile Thr Asp Ile Trp

420 425 430

Thr Tyr Gln Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile

435 440 445

Asp Met Ala Asp Ser Glu Met Leu Asn Leu Tyr Glu Arg Val Arg Lys

450 455 460

Gln Leu Arg Gln Asn Ala Glu Glu Asp Gly Lys Gly Cys Phe Glu Ile

465 470 475 480

Tyr His Ala Cys Asp Asp Ser Cys Met Glu Ser Ile Arg Asn Asn Thr

485 490 495

Tyr Asp His Ser Gln Tyr Arg Glu Glu Ala Leu Leu Asn Arg Leu Asn

500 505 510

Ile Asn Pro Val Ser Ala

515

<210> 32

<211> 229

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 32

Cys Met Lys Gly Arg Lys His Lys Asp Leu Gly Asn Cys His Pro Ile

1 5 10 15

Gly Met Leu Ile Gly Thr Pro Ala Cys Asp Leu His Leu Thr Gly Met

20 25 30

Trp Asp Thr Leu Ile Glu Arg Glu Asn Ala Ile Ala Tyr Cys Phe Pro

35 40 45

Gly Ala Thr Val Asn Val Glu Ala Leu Arg Gln Lys Ile Met Glu Ser

50 55 60

Gly Gly Ile Asn Lys Ile Ser Thr Gly Phe Thr Tyr Gly Ser Ser Ile

65 70 75 80

Asn Ser Ala Gly Thr Thr Arg Ala Cys Met Arg Asn Gly Gly Asn Ser

85 90 95

Phe Tyr Ala Glu Leu Lys Trp Leu Val Ser Lys Ser Lys Gly Gln Asn

100 105 110

Phe Pro Gln Thr Thr Asn Thr Tyr Arg Asn Thr Asp Thr Ala Glu His

115 120 125

Leu Ile Met Trp Gly Ile His His Pro Ser Ser Thr Gln Glu Lys Asn

130 135 140

Asp Leu Tyr Gly Thr Gln Ser Leu Ser Ile Ser Val Gly Ser Ser Thr

145 150 155 160

Tyr Arg Asn Asn Phe Val Pro Val Val Gly Ala Arg Pro Gln Val Asn

165 170 175

Gly Gln Ser Gly Arg Ile Asp Phe His Trp Thr Leu Val Gln Pro Gly

180 185 190

Asp Asn Ile Thr Phe Ser His Asn Gly Gly Leu Ile Ala Pro Ser Arg

195 200 205

Val Ser Lys Leu Ile Gly Arg Gly Leu Gly Ile Gln Ser Asp Ala Pro

210 215 220

Ile Asp Asn Asn Cys

225

<210> 33

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 33

Lys Leu Asn Arg Leu Val Glu Lys Thr Asn Thr Glu Phe Glu Ser Ile

1 5 10 15

Glu Ser Glu Phe Ser Glu Ile Glu His Gln Ile Gly Asn Val Ile Asn

20 25 30

Trp Thr Lys Asp Ser Ile Thr Asp Ile Trp Thr Tyr Gln Ala Glu Leu

35 40 45

Leu Val Ala Met Glu

50

<210> 34

<211> 525

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 34

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Phe Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Gly Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Glu Ser

485 490 495

Ile Arg Asn Glu Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Ile Lys Gly Val Gly Gly Ser Ala

515 520 525

<210> 35

<211> 226

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 35

Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile

1 5 10 15

Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys

20 25 30

Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn Cys Phe Pro

35 40 45

Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser

50 55 60

Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Thr Gly Val Thr

65 70 75 80

Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Gly Ser Ser Ser Ser Phe

85 90 95

Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr Tyr Pro Ala

100 105 110

Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys Leu Tyr Ile

115 120 125

Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile Phe Leu Tyr

130 135 140

Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln

145 150 155 160

Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro

165 170 175

Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu

180 185 190

Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys

195 200 205

Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly

210 215 220

Lys Cys

225

<210> 36

<211> 53

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 36

Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile

1 5 10 15

Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys

20 25 30

Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu

35 40 45

Leu Val Ala Leu Glu

50

<210> 37

<211> 537

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 37

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Ser Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Gly

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr

180 185 190

Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser

195 200 205

Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln

210 215 220

Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln

225 230 235 240

Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser

245 250 255

Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly

260 265 270

Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys

275 280 285

Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly

290 295 300

Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys

305 310 315 320

Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile

325 330 335

Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro

340 345 350

Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly

355 360 365

Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr

370 375 380

Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser

385 390 395 400

Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser

405 410 415

Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu

420 425 430

Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg

435 440 445

Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn

450 455 460

Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg

465 470 475 480

Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly

485 490 495

Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys Ile

500 505 510

Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp

515 520 525

Ser Leu Asn Ile Thr Ala Ala Ser Ala

530 535

<210> 38

<211> 240

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 38

Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val Ala Leu Gly Arg

1 5 10 15

Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val Ser Ile Leu His

20 25 30

Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile Met His Asp Arg

35 40 45

Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly Tyr Glu His Val

50 55 60

Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Gly Ala Pro Gly Gly

65 70 75 80

Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile Thr Asn Gly

85 90 95

Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro Asp Lys Asn Lys

100 105 110

Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr Val Cys Thr Glu

115 120 125

Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser Asp Asn Glu Thr

130 135 140

Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln Lys Phe Thr Ser

145 150 155 160

Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln Ile Gly Gly Phe

165 170 175

Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser Gly Arg Ile Val

180 185 190

Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly Thr Ile Thr Tyr

195 200 205

Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys Ala Ser Gly Arg

210 215 220

Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly Glu Ala Asp Cys

225 230 235 240

<210> 39

<211> 56

<212> PRT

<213> 流感病毒（Influenza Virus）

<400> 39

Lys Asn Leu Asn Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg

1 5 10 15

Leu Ser Gly Ala Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp

20 25 30

Glu Lys Val Asp Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu

35 40 45

Leu Ala Val Leu Leu Ser Asn Glu

50 55

<210> 40

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 40

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg atggaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg aactacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatca aaggctccac aatgcacttc gaatacatct gcgactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacatgcctc 360

accgacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 41

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 41

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Trp Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Lys Gly Ser Thr Met His Phe Glu Tyr Ile Cys Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Cys Leu Thr Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 42

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 42

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 43

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 43

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 44

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 44

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 45

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 45

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 46

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 46

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgaaagggt gaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 47

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 47

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Lys Gly Glu Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 48

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 48

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgaaagggt gaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 49

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 49

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Lys Gly Glu Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 50

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 50

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 51

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 51

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 52

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 52

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 53

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 53

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 54

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 54

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 55

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 55

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 56

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 56

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 57

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 57

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 58

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 58

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgaaagggt gaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 59

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 59

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Lys Gly Glu Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 60

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 60

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgaaagggt gaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 61

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 61

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Lys Gly Glu Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 62

<211> 465

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 62

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg agagaacgca 60

gaaatcatac tcgccctagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac agcacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcct aacaactgag 360

tcagacgaac aagccgaagc ccgcgccgga ctcatcgaag gaaaaatgca caaccacggc 420

gaagactggg gcgcagccgc cgtcgaaatg gccacaaaat tcaac 465

<210> 63

<211> 155

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 63

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Glu Asn Ala Glu Ile Ile Leu Ala Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Ala His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Leu Thr Thr Glu Ser Asp Glu Gln Ala Glu Ala Arg

115 120 125

Ala Gly Leu Ile Glu Gly Lys Met His Asn His Gly Glu Asp Trp Gly

130 135 140

Ala Ala Ala Val Glu Met Ala Thr Lys Phe Asn

145 150 155

<210> 64

<211> 456

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 64

atgggcaaat acgacggatc caaactccgc ataggcatcc tccacgcccg aggaaacgca 60

gaaatcatac tcgaactagt actaggcgcc ctaaaacgcc tccaagaatt cggcgtaaaa 120

cgagaaaaca taatcatcga aacagtcccc ggctcattcg agctacccta cggctcaaaa 180

ctcttcgtcg aaaaacaaaa acgactcgga aaaccactcg acgcaatcat ccccatcgga 240

gtactcatcc gcggctccac accacacttc gactacatcg cagactcaac aacacaccaa 300

ctcatgaaac tcaacttcga actaggcata ccagtcatct tcggagtcat cacagcagac 360

actgacgaac aagccgaaga gcgcgccgga actaaggcag gtaaccacgg cgaagactgg 420

ggcgcagccg ccgtcgaaat ggccacaaaa ttcaac 456

<210> 65

<211> 152

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 65

Met Gly Lys Tyr Asp Gly Ser Lys Leu Arg Ile Gly Ile Leu His Ala

1 5 10 15

Arg Gly Asn Ala Glu Ile Ile Leu Glu Leu Val Leu Gly Ala Leu Lys

20 25 30

Arg Leu Gln Glu Phe Gly Val Lys Arg Glu Asn Ile Ile Ile Glu Thr

35 40 45

Val Pro Gly Ser Phe Glu Leu Pro Tyr Gly Ser Lys Leu Phe Val Glu

50 55 60

Lys Gln Lys Arg Leu Gly Lys Pro Leu Asp Ala Ile Ile Pro Ile Gly

65 70 75 80

Val Leu Ile Arg Gly Ser Thr Pro His Phe Asp Tyr Ile Ala Asp Ser

85 90 95

Thr Thr His Gln Leu Met Lys Leu Asn Phe Glu Leu Gly Ile Pro Val

100 105 110

Ile Phe Gly Val Ile Thr Ala Asp Thr Asp Glu Gln Ala Glu Glu Arg

115 120 125

Ala Gly Thr Lys Ala Gly Asn His Gly Glu Asp Trp Gly Ala Ala Ala

130 135 140

Val Glu Met Ala Thr Lys Phe Asn

145 150

<210> 66

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 66

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgaggaa acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 67

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 67

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Gly Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 68

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 68

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgaggaa acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacaccac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcatcacag cagacactga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 69

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 69

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Gly Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Pro His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Ile Thr Ala

130 135 140

Asp Thr Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 70

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 70

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacaccac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcatcacag cagacactga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 71

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 71

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Pro His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Ile Thr Ala

130 135 140

Asp Thr Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 72

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 72

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 73

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 73

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 74

<211> 540

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 74

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgcc ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagcccgcg ccggactcat cgaaggaaaa 480

atgcacaacc acggcgaaga ctggggcgca gccgccgtcg aaatggccac aaaattcaac 540

<210> 75

<211> 180

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 75

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Ala Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Ala Arg Ala Gly Leu Ile Glu Gly Lys

145 150 155 160

Met His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala

165 170 175

Thr Lys Phe Asn

180

<210> 76

<211> 540

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 76

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgcc ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagcccgcg ccggactcat cgaaggaaaa 480

atgcacaacc acggcgaaga ctggggcgca gccgccgtcg aaatggccac aaaattcaac 540

<210> 77

<211> 180

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 77

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Ala Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Ala Arg Ala Gly Leu Ile Glu Gly Lys

145 150 155 160

Met His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala

165 170 175

Thr Lys Phe Asn

180

<210> 78

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 78

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgaa agggtgaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 79

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 79

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Lys Gly Glu

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 80

<211> 531

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 80

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgaa ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgaa agggtgaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacaccac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcatcacag cagacactga cgaacaagcc gaagagcgcg ccggaactaa ggcaggtaac 480

cacggcgaag actggggcgc agccgccgtc gaaatggcca caaaattcaa c 531

<210> 81

<211> 177

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 81

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Glu Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Lys Gly Glu

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Pro His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Ile Thr Ala

130 135 140

Asp Thr Asp Glu Gln Ala Glu Glu Arg Ala Gly Thr Lys Ala Gly Asn

145 150 155 160

His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala Thr Lys Phe

165 170 175

Asn

<210> 82

<211> 540

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 82

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgaggaa acgcagaaat catactcgcc ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgaa agggtgaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacaccac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcatcacag cagacactga cgaacaagcc gaagcccgcg ccggactcat cgaaggaaaa 480

atgcacaacc acggcgaaga ctggggcgca gccgccgtcg aaatggccac aaaattcaac 540

<210> 83

<211> 180

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 83

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Gly Asn Ala Glu Ile Ile

35 40 45

Leu Ala Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Lys Gly Glu

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Pro His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Ile Thr Ala

130 135 140

Asp Thr Asp Glu Gln Ala Glu Ala Arg Ala Gly Leu Ile Glu Gly Lys

145 150 155 160

Met His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala

165 170 175

Thr Lys Phe Asn

180

<210> 84

<211> 540

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 84

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgagaga acgcagaaat catactcgcc ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgac tcggaaaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacaccac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcatcacag cagacactga cgaacaagcc gaagcccgcg ccggactcat cgaaggaaaa 480

atgcacaacc acggcgaaga ctggggcgca gccgccgtcg aaatggccac aaaattcaac 540

<210> 85

<211> 180

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 85

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Glu Asn Ala Glu Ile Ile

35 40 45

Leu Ala Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Leu Gly Lys

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Pro His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Ile Thr Ala

130 135 140

Asp Thr Asp Glu Gln Ala Glu Ala Arg Ala Gly Leu Ile Glu Gly Lys

145 150 155 160

Met His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala

165 170 175

Thr Lys Phe Asn

180

<210> 86

<211> 540

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 86

atggaaacag atactctttt gttgtgggtt ctcctcctgt gggttccagg gtcaaccggg 60

gactacaagg acgagatggg caaatacgac ggatccaaac tccgcatagg catcctccac 120

gcccgaggaa acgcagaaat catactcgcc ctagtactag gcgccctaaa acgcctccaa 180

gaattcggcg taaaacgaga aaacataatc atcgaaacag tccccggctc attcgagcta 240

ccctacggct caaaactctt cgtcgaaaaa caaaaacgaa agggtgaacc actcgacgca 300

atcatcccca tcggagtact catccgcggc tccacagcac acttcgacta catcgcagac 360

tcaacaacac accaactcat gaaactcaac ttcgaactag gcataccagt catcttcgga 420

gtcctaacaa ctgagtcaga cgaacaagcc gaagcccgcg ccggactcat cgaaggaaaa 480

atgcacaacc acggcgaaga ctggggcgca gccgccgtcg aaatggccac aaaattcaac 540

<210> 87

<211> 180

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 87

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro

1 5 10 15

Gly Ser Thr Gly Asp Tyr Lys Asp Glu Met Gly Lys Tyr Asp Gly Ser

20 25 30

Lys Leu Arg Ile Gly Ile Leu His Ala Arg Gly Asn Ala Glu Ile Ile

35 40 45

Leu Ala Leu Val Leu Gly Ala Leu Lys Arg Leu Gln Glu Phe Gly Val

50 55 60

Lys Arg Glu Asn Ile Ile Ile Glu Thr Val Pro Gly Ser Phe Glu Leu

65 70 75 80

Pro Tyr Gly Ser Lys Leu Phe Val Glu Lys Gln Lys Arg Lys Gly Glu

85 90 95

Pro Leu Asp Ala Ile Ile Pro Ile Gly Val Leu Ile Arg Gly Ser Thr

100 105 110

Ala His Phe Asp Tyr Ile Ala Asp Ser Thr Thr His Gln Leu Met Lys

115 120 125

Leu Asn Phe Glu Leu Gly Ile Pro Val Ile Phe Gly Val Leu Thr Thr

130 135 140

Glu Ser Asp Glu Gln Ala Glu Ala Arg Ala Gly Leu Ile Glu Gly Lys

145 150 155 160

Met His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala

165 170 175

Thr Lys Phe Asn

180

<210> 88

<211> 357

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 88

gaggaggccg agctggccta cctgctgggc gagctggcct acaagctggg cgagtatcgg 60

atcgccatca gggcatacag gatcgccctg aagcgggacc ccaacaatgc cgaggcctgg 120

tacaacctgg gcaatgccta ctataagcag ggccggtata gagaggccat cgagtactat 180

cagaaggccc tggagctgga ccccaataac gctgaagctt ggtataacct gggcaacgct 240

tactatgagc ggggcgagta tgaggaagct atcgagtact atcggaaggc cctgagactg 300

gaccccaata acgccgatgc catgcagaac ctgctgaacg caaagatgag agaagaa 357

<210> 89

<211> 119

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 89

Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu

1 5 10 15

Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg

20 25 30

Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr

35 40 45

Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu

50 55 60

Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala

65 70 75 80

Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys

85 90 95

Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu

100 105 110

Asn Ala Lys Met Arg Glu Glu

115

<210> 90

<211> 1917

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 90

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaat acctgcatgg aaagcgtgaa aaacgggacc 1500

tacgattacc ctaaatacag cgaggaagcc aaactgaaca gggagggatc cggctccgga 1560

gaggaggccg agctggccta cctgctgggc gagctggcct acaagctggg cgagtatagg 1620

atcgccatca gggcatacag gatcgccctg aagcgcgacc ccaacaatgc cgaggcctgg 1680

tacaacctgg gcaatgccta ctataagcag ggccggtata gagaggccat cgagtactat 1740

cagaaggccc tggagctgga ccccaataac gctgaagctt ggtataacct gggcaacgct 1800

tactatgagc ggggcgagta tgaggaagct atcgagtact atcggaaggc cctgagactg 1860

gaccccaaca atgccgatgc catgcagaat ctgctgaatg ccaaaatgag agaggag 1917

<210> 91

<211> 639

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 91

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Gly Ser Gly Ser Gly Glu Glu Ala Glu Leu Ala Tyr Leu

515 520 525

Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg

530 535 540

Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp

545 550 555 560

Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala

565 570 575

Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu

580 585 590

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu

595 600 605

Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn

610 615 620

Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 92

<211> 1932

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 92

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa atgcgacaac acctgtatgg aaagcgtgaa aaatgggact 1500

tacgactacc ctaaatactc agaggaagcc aaactgaacc gggagaagat cgacggagtg 1560

ggaggagcta gcgccgagga ggccgagctg gcctacctgc tgggcgagct ggcctacaag 1620

ctgggcgagt ataggatcgc catcagggca tacaggatcg ccctgaagcg cgaccccaac 1680

aatgccgagg cctggtacaa cctgggcaat gcctactata agcagggccg gtatagagag 1740

gccatcgagt actatcagaa ggccctggag ctggacccca ataacgctga agcttggtat 1800

aacctgggca acgcttacta tgagcggggc gagtatgagg aagctatcga gtactatcgg 1860

aaggccctga gactggaccc caacaatgcc gatgccatgc agaatctgct gaacgctaaa 1920

atgcgagaag aa 1932

<210> 93

<211> 644

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 93

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Val Gly Gly Ala Ser Ala Glu Glu Ala

515 520 525

Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr

530 535 540

Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn

545 550 555 560

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly

565 570 575

Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp

580 585 590

Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu

595 600 605

Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg

610 615 620

Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys

625 630 635 640

Met Arg Glu Glu

<210> 94

<211> 1935

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 94

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtattc cgaggaggcc aagctgaacc gggagaagat cgacggcgtg 1560

ccccctggcg ctagcgccga ggaggccgag ctggcctacc tgctgggcga gctggcctac 1620

aagctgggcg agtataggat cgccatcagg gcatacagga tcgccctgaa gcgcgacccc 1680

aacaatgccg aggcctggta caacctgggc aatgcctact ataagcaggg ccggtataga 1740

gaggccatcg agtactatca gaaggccctg gagctggacc ccaataacgc tgaagcttgg 1800

tataacctgg gcaacgctta ctatgagcgg ggcgagtatg aggaagctat cgagtactat 1860

cggaaggccc tgagactgga ccccaacaat gccgatgcca tgcagaatct gctgaacgct 1920

aaaatgcgag aagaa 1935

<210> 95

<211> 645

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 95

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Val Pro Pro Gly Ala Ser Ala Glu Glu

515 520 525

Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu

530 535 540

Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro

545 550 555 560

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln

565 570 575

Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu

580 585 590

Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr

595 600 605

Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu

610 615 620

Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala

625 630 635 640

Lys Met Arg Glu Glu

645

<210> 96

<211> 1935

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 96

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtattc cgaggaggcc aagctgaacc gggagaagat cgacggcttt 1560

ccccctggcg ctagcgccga ggaggccgag ctggcctacc tgctgggcga gctggcctac 1620

aagctgggcg agtataggat cgccatcagg gcatacagga tcgccctgaa gcgcgacccc 1680

aacaatgccg aggcctggta caacctgggc aatgcctact ataagcaggg ccggtataga 1740

gaggccatcg agtactatca gaaggccctg gagctggacc ccaataacgc tgaagcttgg 1800

tataacctgg gcaacgctta ctatgagcgg ggcgagtatg aggaagctat cgagtactat 1860

cggaaggccc tgagactgga ccccaacaat gccgatgcca tgcagaatct gctgaacgct 1920

aaaatgcgag aagaa 1935

<210> 97

<211> 645

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 97

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Phe Pro Pro Gly Ala Ser Ala Glu Glu

515 520 525

Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu

530 535 540

Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro

545 550 555 560

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln

565 570 575

Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu

580 585 590

Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr

595 600 605

Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu

610 615 620

Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala

625 630 635 640

Lys Met Arg Glu Glu

645

<210> 98

<211> 1935

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 98

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtattc cgaggaggcc aagctgaacc gggagaagat cgacggcatc 1560

ccccctggcg ctagcgccga ggaggccgag ctggcctacc tgctgggcga gctggcctac 1620

aagctgggcg agtataggat cgccatcagg gcatacagga tcgccctgaa gcgcgacccc 1680

aacaatgccg aggcctggta caacctgggc aatgcctact ataagcaggg ccggtataga 1740

gaggccatcg agtactatca gaaggccctg gagctggacc ccaataacgc tgaagcttgg 1800

tataacctgg gcaacgctta ctatgagcgg ggcgagtatg aggaagctat cgagtactat 1860

cggaaggccc tgagactgga ccccaacaat gccgatgcca tgcagaatct gctgaacgct 1920

aaaatgcgag aagaa 1935

<210> 99

<211> 645

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 99

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Ile Pro Pro Gly Ala Ser Ala Glu Glu

515 520 525

Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu

530 535 540

Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro

545 550 555 560

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln

565 570 575

Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu

580 585 590

Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr

595 600 605

Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu

610 615 620

Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala

625 630 635 640

Lys Met Arg Glu Glu

645

<210> 100

<211> 1935

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 100

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtattc cgaggaggcc aagctgaacc gggagaagat cgacggctgg 1560

ccccctggcg ctagcgccga ggaggccgag ctggcctacc tgctgggcga gctggcctac 1620

aagctgggcg agtataggat cgccatcagg gcatacagga tcgccctgaa gcgcgacccc 1680

aacaatgccg aggcctggta caacctgggc aatgcctact ataagcaggg ccggtataga 1740

gaggccatcg agtactatca gaaggccctg gagctggacc ccaataacgc tgaagcttgg 1800

tataacctgg gcaacgctta ctatgagcgg ggcgagtatg aggaagctat cgagtactat 1860

cggaaggccc tgagactgga ccccaacaat gccgatgcca tgcagaatct gctgaacgct 1920

aaaatgcgag aagaa 1935

<210> 101

<211> 645

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 101

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Trp Pro Pro Gly Ala Ser Ala Glu Glu

515 520 525

Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu

530 535 540

Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro

545 550 555 560

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln

565 570 575

Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu

580 585 590

Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr

595 600 605

Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu

610 615 620

Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala

625 630 635 640

Lys Met Arg Glu Glu

645

<210> 102

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 102

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gagagaagat cgacggcgtg 1560

tctgccgagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tataggatcg ccatcagggc ctatcgcatc gccctgaagc gcgaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagcgggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 103

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 103

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Val Ser Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 104

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 104

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gagagaagat cgacggagtg 1560

aaggcagagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tataggatcg ccatcagggc ctatcgcatc gccctgaagc gcgaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagcgggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 105

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 105

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Val Lys Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 106

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 106

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gggagaagat cgacggcgtg 1560

cgcgcagagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tatcggatcg ccatcagggc ctatcgcatc gccctgaagc gggaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagagggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 107

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 107

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Val Arg Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 108

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 108

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gagagaagat cgacggcttt 1560

tctgccgagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tataggatcg ccatcagggc ctatcgcatc gccctgaagc gcgaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagcgggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 109

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 109

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Phe Ser Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 110

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 110

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gagagaagat cgacggcttt 1560

aaggcagagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tataggatcg ccatcagggc ctatcgcatc gccctgaagc gcgaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagcgggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 111

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 111

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Phe Lys Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 112

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 112

atgaaggcaa tcctggtcgt cctgctgtac actttcacta ctgctaacgc tgacactctg 60

tgcatcgggt atcacgccaa caactccacc gacacagtgg ataccgtgct ggagaagaac 120

gtgaccgtga cacactctgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180

ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgccggctg gattctgggc 240

aatcctgagt gtgagtctct gagcaccgcc agctcctggt cctacatcgt ggagacaagc 300

aactctgaca atggcacatg ctttcccggc gatttcatca actatgagga gctgcgggag 360

cagctgtcta gcgtgtccag cttcgagcgg ttcgagattt tcccaaagac cagctcctgg 420

cccaaccacg actccaataa gggagtgaca gcagcatgtc ctcacgcagg cgccaagtct 480

ttttacaaga acctgatctg gctggtgaag aagggcaatt cttacccaaa gctgaaccag 540

agctatatca atgataaggg caaggaggtg ctggtgctgt ggggcatcca ccacccttcc 600

accacagccg accagcagtc tctgtaccag aacgccgatg cctacgtgtt cgtgggcacc 660

agccggtact ccaagaagtt caagcctgag atcgccacac ggccaaaggt gcgggaccag 720

gagggcagaa tgaattacta ttggaccctg gtggagcctg gcgataagat cacctttgag 780

gccacaggca acctggtggt gccacggtat gccttcacca tggagagaaa tgccggctcc 840

ggcatcatca tctctgacac accagtgcac gattgcaaca ccacatgtca gacccccgag 900

ggcgccatca acacaagcct gcccttccag aatatccacc ctatcaccat cggcaagtgc 960

ccaaagtacg tgaagtccac caagctgagg ctggccacag gcctgcgcaa tgtgccctct 1020

atccagagca ggggcctgtt tggagcaatc gcaggcttca tcgagggagg atggacagga 1080

atggtggacg gatggtacgg ctatcaccac cagaacgagc agggcagcgg atatgcagcc 1140

gacctgaagt ccacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200

gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260

atcgagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320

gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380

aatctgtatg agaaggtgcg caaccagctg aagaacaatg ccaaggagat cggcaatggc 1440

tgtttcgaat tctaccacaa gtgcgacaac acctgtatgg agagcgtgaa gaatggcaca 1500

tacgattatc ccaagtactc cgaggaggcc aagctgaaca gggagaagat cgacggcttt 1560

cgcgcagagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tatcggatcg ccatcagggc ctatcgcatc gccctgaagc gggaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagagggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc caaacaatgc cgatgccatg cagaacctgc tgaatgcaaa gatgagggag 1920

gag 1923

<210> 113

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 113

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn

1 5 10 15

Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val

50 55 60

Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Phe Pro Gly Asp Phe

100 105 110

Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp

130 135 140

Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser

145 150 155 160

Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro

165 170 175

Lys Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val

180 185 190

Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu

195 200 205

Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser

210 215 220

Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln

225 230 235 240

Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys

245 250 255

Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe

260 265 270

Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro

275 280 285

Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn

290 295 300

Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys

305 310 315 320

Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg

325 330 335

Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly

340 345 350

Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr

355 360 365

His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser

370 375 380

Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile

385 390 395 400

Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His

405 410 415

Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe

420 425 430

Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn

435 440 445

Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu

450 455 460

Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly

465 470 475 480

Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val

485 490 495

Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu

500 505 510

Asn Arg Glu Lys Ile Asp Gly Phe Arg Ala Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 114

<211> 1923

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 114

atgaagacta ttattgccct gtcctacatc ctgtgcctgg tgtttgctca gaaaatccct 60

gggaacgaca actccactgc taccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgacagaatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

accctgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt acccctatga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttccaggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccat cccgcatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgcggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaatgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggcttcaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaacgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aataagctgt tcgaaaagac taaaaaacag ctgcgggaaa acgccgagga catgggcaat 1440

ggatgcttca aaatctatca taagtgcgac aacgcctgta ttggctctat caggaacggc 1500

acctacgacc acaacgtgta cagggatgag gccctgaaca atcgcttcca gggatccggc 1560

tccggagagg aggcagagct ggcctacctg ctgggcgagc tggcctacaa gctgggcgag 1620

tatcggatcg ccatcagggc atacaggatc gccctgaagc gggaccccaa caatgccgag 1680

gcctggtaca acctgggcaa tgcctactat aagcagggcc ggtatagaga ggccatcgag 1740

tactatcaga aggccctgga gctggacccc aataacgctg aagcttggta taacctgggc 1800

aacgcttact atgagcgggg cgagtatgag gaagctatcg agtactatcg gaaggccctg 1860

agactggacc ccaataacgc cgatgccatg cagaacctgc tgaacgctaa gatgagagag 1920

gag 1923

<210> 115

<211> 641

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 115

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Gly Ser Gly Ser Gly Glu Glu Ala Glu Leu Ala

515 520 525

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

530 535 540

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

545 550 555 560

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

565 570 575

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

580 585 590

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

595 600 605

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

610 615 620

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

625 630 635 640

Glu

<210> 116

<211> 1926

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 116

atgaagacta ttattgccct gtcctacatc ctgtgcctgg tgtttgctca gaaaatccct 60

gggaacgaca actccactgc taccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgacagaatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

accctgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt acccctatga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttccaggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccat cccgcatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgcggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaatgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggcttcaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaacgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aataagctgt tcgaaaaaac caagaaacag ctgagggaaa acgccgagga catgggcaac 1440

gggtgcttca aaatctatca caaatgcgac aacgcctgta ttggcagcat caggaacggc 1500

acctacgacc acaacgtgta cagggatgag gccctgaaca atcgcttcgg accacctgga 1560

gctagcgccg aggaggccga gctggcctac ctgctgggcg agctggccta caagctgggc 1620

gagtatcgga tcgccatcag ggcatacagg atcgccctga agcgggaccc caacaatgcc 1680

gaggcctggt acaacctggg caatgcctac tataagcagg gccggtatag agaggccatc 1740

gagtactatc agaaggccct ggagctggac cccaataacg ctgaagcttg gtataacctg 1800

ggcaacgctt actatgagcg gggcgagtat gaggaagcta tcgagtacta tcggaaggcc 1860

ctgagactgg accccaataa cgccgatgcc atgcagaacc tgctgaacgc aaagatgaga 1920

gaagaa 1926

<210> 117

<211> 642

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 117

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gly Pro Pro Gly Ala Ser Ala Glu Glu Ala Glu Leu

515 520 525

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

530 535 540

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala

545 550 555 560

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr

565 570 575

Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn

580 585 590

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly

595 600 605

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

610 615 620

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg

625 630 635 640

Glu Glu

<210> 118

<211> 1929

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 118

atgaagacta ttattgccct gtcctacatc ctgtgcctgg tgtttgctca gaaaatccct 60

gggaacgaca actccactgc taccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgacagaatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

accctgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt acccctatga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttccaggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccat cccgcatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgcggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaatgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggcttcaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaacgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aataagctgt tcgaaaaaac caagaaacag ctgcgcgaga acgccgaaga catgggcaat 1440

gggtgtttta aaatctacca caaatgcgat aacgcttgta tcgggagcat ccggaacgga 1500

acatacgacc ataatgtcta tagggatgag gccctgaaca atcgctttca gggcccccca 1560

ggagctagcg ccgaggaggc cgagctggcc tacctgctgg gcgagctggc ctacaagctg 1620

ggcgagtatc ggatcgccat cagggcatac aggatcgccc tgaagcggga ccccaacaat 1680

gccgaggcct ggtacaacct gggcaatgcc tactataagc agggccggta tagagaggcc 1740

atcgagtact atcagaaggc cctggagctg gaccccaata acgctgaagc ttggtataac 1800

ctgggcaacg cttactatga gcggggcgag tatgaggaag ctatcgagta ctatcggaag 1860

gccctgagac tggaccccaa taacgccgat gccatgcaga acctgctgaa cgcaaagatg 1920

agagaagaa 1929

<210> 119

<211> 643

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 119

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Gly Pro Pro Gly Ala Ser Ala Glu Glu Ala Glu

515 520 525

Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg

530 535 540

Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn

545 550 555 560

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg

565 570 575

Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro

580 585 590

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg

595 600 605

Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu

610 615 620

Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met

625 630 635 640

Arg Glu Glu

<210> 120

<211> 1929

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 120

atgaagacta ttattgccct gtcctacatc ctgtgcctgg tgtttgctca gaaaatccct 60

gggaacgaca actccactgc taccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgacagaatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

accctgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt acccctatga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttccaggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccat cccgcatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgcggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaatgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggcttcaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaacgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aataagctgt tcgaaaaaac caagaaacag ctgagggaga acgcagaaga catgggcaat 1440

gggtgtttta aaatctacca caaatgcgat aacgcttgta tcgggagcat tcgcaatgga 1500

acatacgacc ataatgtcta cagggatgag gccctgaaca atcgcttcca gattccacca 1560

ggcgctagcg ccgaggaggc cgagctggcc tacctgctgg gcgagctggc ctacaagctg 1620

ggcgagtatc ggatcgccat cagggcatac aggatcgccc tgaagcggga ccccaacaat 1680

gccgaggcct ggtacaacct gggcaatgcc tactataagc agggccggta tagagaggcc 1740

atcgagtact atcagaaggc cctggagctg gaccccaata acgctgaagc ttggtataac 1800

ctgggcaacg cttactatga gcggggcgag tatgaggaag ctatcgagta ctatcggaag 1860

gccctgagac tggaccccaa taacgccgat gccatgcaga acctgctgaa cgcaaagatg 1920

agagaagaa 1929

<210> 121

<211> 643

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 121

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Ile Pro Pro Gly Ala Ser Ala Glu Glu Ala Glu

515 520 525

Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg

530 535 540

Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn

545 550 555 560

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg

565 570 575

Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro

580 585 590

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg

595 600 605

Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu

610 615 620

Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met

625 630 635 640

Arg Glu Glu

<210> 122

<211> 1926

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 122

atgaaaacta ttatcgccct gtcatacatc ctgtgcctgg tctttgctca gaaaatccct 60

ggaaacgaca actccaccgc caccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgaccgcatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

acactgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt tcccctacga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttcccggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccct ccagaatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgaggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaacgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggctttaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaatgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aacaagctgt tcgaaaagac caagaagcag ctgagggaga acgccgagga catgggcaat 1440

ggctgcttca aaatctacca caagtgcgat aacgcctgta tcggcagcat ccgcaacggc 1500

acatacgacc acaacgtgta cagggatgag gccctgaaca atcgctttca gatcaagggc 1560

gtgtccgccg aggaggcaga gctggcctac ctgctgggcg agctggccta caagctgggc 1620

gagtatcgga tcgccatcag ggcctatcgc atcgccctga agcgggaccc caacaatgcc 1680

gaggcctggt acaacctggg caatgcctac tataagcagg gccggtatag agaggccatc 1740

gagtactatc agaaggccct ggagctggac cccaataacg ctgaagcttg gtataacctg 1800

ggcaacgctt actatgagcg gggcgagtat gaggaagcta tcgagtacta tcggaaggcc 1860

ctgagactgg accccaataa cgccgatgcc atgcagaacc tgctgaatgc caagatgaga 1920

gaggag 1926

<210> 123

<211> 642

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 123

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Phe Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Ile Lys Gly Val Ser Ala Glu Glu Ala Glu Leu

515 520 525

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

530 535 540

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala

545 550 555 560

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr

565 570 575

Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn

580 585 590

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly

595 600 605

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

610 615 620

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg

625 630 635 640

Glu Glu

<210> 124

<211> 1842

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 124

ctgtgtctgg gccaccacgc cgtgccaaat ggcaccatcg tgaagaccat cacaaacgac 60

cgcatcgagg tgaccaatgc cacagagctg gtgcagaaca gctccatcgg cgagatttgc 120

gacagccccc accagatcct ggatggcgag aactgtacac tgatcgacgc actgctgggc 180

gaccctcagt gcgatggctt ccagaataag aagtgggatc tgtttgtgga gcgcagcaag 240

gcctactcca actgtttccc ctacgacgtg cctgattatg cctctctgcg gagcctggtg 300

gcatctagcg gcaccctgga gttcaacaat gagagcttta attggaccgg cgtgacacag 360

aacggcacat cctctgcctg catccggaga agctcctcta gcttcttttc ccggctgaac 420

tggctgaccc acctgaatta cacatatcct gccctgaatg tgaccatgcc aaacaatgag 480

cagttcgata agctgtacat ctggggagtg caccacccag gaacagacaa ggatcagatt 540

ttcctgtatg cccagtcctc tggccgcatc accgtgtcta caaagcggag ccagcaggcc 600

gtgatcccta acatcggctc taggccacgc atccgggaca tcccctccag aatctctatc 660

tactggacca tcgtgaagcc aggcgatatc ctgctgatca actccacagg caatctgatc 720

gcccccagag gctatttcaa gatcaggtcc ggcaagagct ccatcatgag gtctgacgcc 780

cccatcggca agtgcaagag cgagtgtatc acccctaacg gctccatccc aaatgataag 840

ccctttcaga acgtgaatcg catcacatac ggcgcctgtc ctcggtacgt gaagcacagc 900

accctgaagc tggccacagg catgagaaac gtgccagaga agcagaccag gggaatcttc 960

ggagcaatcg caggctttat cgagaatggc tgggagggca tggtggacgg ctggtacggc 1020

tttagacacc agaactctga gggaagggga caggcagccg acctgaagag cacccaggcc 1080

gccatcgatc agatcaacgg caagctgaat agactgatcg gcaagacaaa tgagaagttc 1140

caccagatcg agaaggagtt tagcgaggtg gagggcagaa tccaggacct ggagaagtac 1200

gtggaggaca ccaagatcga tctgtggtcc tataatgccg agctgctggt ggccctggag 1260

aaccagcaca ccatcgacct gacagattct gagatgaaca agctgttcga aaagaccaag 1320

aagcagctga gggagaacgc cgaggacatg ggcaatggct gcttcaaaat ctaccacaag 1380

tgcgataacg cctgtatcgg cagcatccgc aacggcacat acgaccacaa cgtgtacagg 1440

gatgaggccc tgaacaatcg ctttcagatc aagggcgtgt ccgccgagga ggcagagctg 1500

gcctacctgc tgggcgagct ggcctacaag ctgggcgagt atcggatcgc catcagggcc 1560

tatcgcatcg ccctgaagcg ggaccccaac aatgccgagg cctggtacaa cctgggcaat 1620

gcctactata agcagggccg gtatagagag gccatcgagt actatcagaa ggccctggag 1680

ctggacccca ataacgctga agcttggtat aacctgggca acgcttacta tgagcggggc 1740

gagtatgagg aagctatcga gtactatcgg aaggccctga gactggaccc caataacgcc 1800

gatgccatgc agaacctgct gaatgccaag atgagagagg ag 1842

<210> 125

<211> 634

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 125

Met Asn Thr Gln Ile Leu Val Phe Ala Leu Ile Ala Ile Ile Pro Thr

1 5 10 15

Asn Ala Asp Lys Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr

20 25 30

Ile Val Lys Thr Ile Thr Asn Asp Arg Ile Glu Val Thr Asn Ala Thr

35 40 45

Glu Leu Val Gln Asn Ser Ser Ile Gly Glu Ile Cys Asp Ser Pro His

50 55 60

Gln Ile Leu Asp Gly Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly

65 70 75 80

Asp Pro Gln Cys Asp Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val

85 90 95

Glu Arg Ser Lys Ala Tyr Ser Asn Cys Phe Pro Tyr Asp Val Pro Asp

100 105 110

Tyr Ala Ser Leu Arg Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe

115 120 125

Asn Asn Glu Ser Phe Asn Trp Thr Gly Val Thr Gln Asn Gly Thr Ser

130 135 140

Ser Ala Cys Ile Arg Arg Ser Ser Ser Ser Phe Phe Ser Arg Leu Asn

145 150 155 160

Trp Leu Thr His Leu Asn Tyr Thr Tyr Pro Ala Leu Asn Val Thr Met

165 170 175

Pro Asn Asn Glu Gln Phe Asp Lys Leu Tyr Ile Trp Gly Val His His

180 185 190

Pro Gly Thr Asp Lys Asp Gln Ile Phe Leu Tyr Ala Gln Ser Ser Gly

195 200 205

Arg Ile Thr Val Ser Thr Lys Arg Ser Gln Gln Ala Val Ile Pro Asn

210 215 220

Ile Gly Ser Arg Pro Arg Ile Arg Asp Ile Pro Ser Arg Ile Ser Ile

225 230 235 240

Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr

245 250 255

Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys

260 265 270

Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys Cys Lys Ser Glu

275 280 285

Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn

290 295 300

Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys His Ser

305 310 315 320

Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr

325 330 335

Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu

340 345 350

Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly

355 360 365

Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln

370 375 380

Ile Asn Gly Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu Lys Phe

385 390 395 400

His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp

405 410 415

Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn

420 425 430

Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr

435 440 445

Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln Leu Arg

450 455 460

Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys

465 470 475 480

Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His

485 490 495

Asn Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly

500 505 510

Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala

515 520 525

Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala

530 535 540

Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn

545 550 555 560

Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln

565 570 575

Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu

580 585 590

Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr

595 600 605

Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp Ala Met Gln

610 615 620

Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630

<210> 126

<211> 1932

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 126

atgaaaacta ttatcgccct gtcatacatc ctgtgcctgg tctttgctca gaaaatccct 60

ggaaacgaca actccaccgc caccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgaccgcatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

acactgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt tcccctacga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa caatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagctcc 480

tctagcttct tttcccggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa tgagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccct ccagaatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgaggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaacgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggctttaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaatgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agaatccagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aacaagctgt tcgaaaagac caagaagcag ctgagggaga acgccgagga catgggcaat 1440

ggctgcttca aaatctacca caagtgcgat aacgcctgta tcggcagcat ccgcaacggc 1500

acatacgacc acaacgtgta cagggatgag gccctgaaca atcgctttca gatcaagggc 1560

gtgggcggct ccgccgagga ggcagagctg gcctacctgc tgggcgagct ggcctacaag 1620

ctgggcgagt atcggatcgc catcagggcc tatcgcatcg ccctgaagcg ggaccccaac 1680

aatgccgagg cctggtacaa cctgggcaat gcctactata agcagggccg gtatagagag 1740

gccatcgagt actatcagaa ggccctggag ctggacccca ataacgctga agcttggtat 1800

aacctgggca acgcttacta tgagcggggc gagtatgagg aagctatcga gtactatcgg 1860

aaggccctga gactggaccc caataacgcc gatgccatgc agaacctgct gaatgccaag 1920

atgagagagg ag 1932

<210> 127

<211> 644

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 127

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Phe Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser

485 490 495

Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Ile Lys Gly Val Gly Gly Ser Ala Glu Glu Ala

515 520 525

Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr

530 535 540

Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn

545 550 555 560

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly

565 570 575

Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp

580 585 590

Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu

595 600 605

Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg

610 615 620

Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys

625 630 635 640

Met Arg Glu Glu

<210> 128

<211> 1974

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 128

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc ctccgccgag 1620

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtataggatc 1680

gccatcaggg catacaggat cgccctgaag cgcgacccca acaatgccga ggcctggtac 1740

aacctgggca atgcctacta taagcagggc cggtatagag aggccatcga gtactatcag 1800

aaggccctgg agctggaccc caataacgct gaagcttggt ataacctggg caacgcttac 1860

tatgagcggg gcgagtatga ggaagctatc gagtactatc ggaaggccct gagactggac 1920

ccaaacaatg ccgatgccat gcagaacctg ctgaatgcaa agatgaggga ggag 1974

<210> 129

<211> 658

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 129

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu

530 535 540

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

545 550 555 560

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala

565 570 575

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr

580 585 590

Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn

595 600 605

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly

610 615 620

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

625 630 635 640

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg

645 650 655

Glu Glu

<210> 130

<211> 1980

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 130

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc cagcctgtcc 1620

gccgaggagg cagagctggc ctacctgctg ggcgagctgg cctacaagct gggcgagtat 1680

aggatcgcca tcagggcata caggatcgcc ctgaagcgcg accccaacaa tgccgaggcc 1740

tggtacaacc tgggcaatgc ctactataag cagggccggt atagagaggc catcgagtac 1800

tatcagaagg ccctggagct ggaccccaat aacgctgaag cttggtataa cctgggcaac 1860

gcttactatg agcggggcga gtatgaggaa gctatcgagt actatcggaa ggccctgaga 1920

ctggacccaa acaatgccga tgccatgcag aacctgctga atgcaaagat gagggaggag 1980

<210> 131

<211> 660

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 131

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Ser Ala Glu Glu Ala

530 535 540

Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr

545 550 555 560

Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn

565 570 575

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly

580 585 590

Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp

595 600 605

Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu

610 615 620

Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg

625 630 635 640

Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys

645 650 655

Met Arg Glu Glu

660

<210> 132

<211> 1971

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 132

atgaaagcta ttatcgtgct gctgatggtc gtgacttcca acgctgacag gatttgcaca 60

ggcattacct ccagtaactc cccacatgtg gtgaagacag ccacccaggg cgaagtgaat 120

gtgaccggcg tgatccctct gaccacaacc ccaacaaagt cctacttcgc caatctgaag 180

ggcacacgga ccagaggcaa gctgtgccct gactgtctga actgcaccga cctggatgtg 240

gccctgggca ggcctatgtg cgtgggaaca accccatccg ccaaggcctc tatcctgcac 300

gaggtgcgcc ccgtgacctc cggatgcttt cctatcatgc acgacaggac aaagatccgc 360

cagctgccaa atctgctgcg gggctacgag aagatcagac tgtctacaca gaacgtgatc 420

gatgcagaga aggcaccagg aggaccatat cggctgggca catccggctc ttgtcccaat 480

gccaccagca agatcggctt ctttgccaca atggcctggg ccgtgcctaa ggacaactac 540

aagaatgcca caaaccccct gaccgtggag gtgccttata tctgcacaga gggcgaggac 600

cagatcaccg tgtggggctt ccacagcgat aacaagaccc agatgaagag cctgtacggc 660

gattccaatc cccagaagtt cacaagctcc gccaacggcg tgacaaccca ctatgtgagc 720

cagatcggcg actttccaga tcagaccgag gacggaggcc tgccacagtc cggcaggatc 780

gtggtggatt acatgatgca gaagcctggc aagacaggca ccatcgtgta tcagcggggc 840

gtgctgctgc cacagaaagt gtggtgtgcc tctggccgga gcaaagtgat caagggctct 900

ctgccactga tcggcgaggc cgactgcctg cacgaggagt atggcggcct gaataagagc 960

aagccctact ataccggcaa gcacgccaag gccatcggca attgtccaat ctgggtgaag 1020

acccccctga agctggccaa cggcacaaag tacaggccac ctgcaaagct gctgaaggag 1080

aggggcttct ttggagcaat cgcaggcttt ctggagggag gatgggaggg aatgatcgca 1140

ggatggcacg gatatacctc ccacggagca cacggagtgg cagtggcagc cgacctgaag 1200

tctacccagg aggccatcaa caagatcaca aagaacctga atagcctgtc cgagctggag 1260

gtgaagaatc tgcagcggct gtccggcgcc atggatgagc tgcacaacga gatcctggag 1320

ctggacgaga aggtggacga tctgagagcc gataccatct ctagccagat cgagctggcc 1380

gtgctgctgt ctaatgaggg catcatcaac agcgaggatg agcacctgct ggccctggag 1440

aggaagctga agaagatgct gggcccttct gccgtggaca tcggcaatgg ctgcttcgaa 1500

accaagcaca agtgcaacca gacatgtctg gacagaatcg ccgccggcac cttcaatgcc 1560

ggcgagtttt ctctgcccac cttcgatagc ctgaacatca cagcagcctc cgccgaggag 1620

gcagagctgg cctacctgct gggcgagctg gcctacaagc tgggcgagta taggatcgcc 1680

atcagggcat acaggatcgc cctgaagcgc gaccccaaca atgccgaggc ctggtacaac 1740

ctgggcaatg cctactataa gcagggccgg tatagagagg ccatcgagta ctatcagaag 1800

gccctggagc tggaccccaa taacgctgaa gcttggtata acctgggcaa cgcttactat 1860

gagcggggcg agtatgagga agctatcgag tactatcgga aggccctgag actggaccca 1920

aacaatgccg atgccatgca gaacctgctg aatgcaaaga tgagggagga g 1971

<210> 133

<211> 657

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 133

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr

50 55 60

Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys

130 135 140

Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro

180 185 190

Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His

195 200 205

Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro

210 215 220

Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser

225 230 235 240

Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln

245 250 255

Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr

260 265 270

Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp

275 280 285

Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile

290 295 300

Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser

305 310 315 320

Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro

325 330 335

Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg

340 345 350

Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala

355 360 365

Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly

370 375 380

Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys

385 390 395 400

Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu

405 410 415

Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp

420 425 430

Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu

435 440 445

Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser

450 455 460

Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu

465 470 475 480

Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn

485 490 495

Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg

500 505 510

Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe

515 520 525

Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu Ala

530 535 540

Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala

545 550 555 560

Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu

565 570 575

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg

580 585 590

Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn

595 600 605

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu

610 615 620

Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro

625 630 635 640

Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu

645 650 655

Glu

<210> 134

<211> 1977

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 134

atgaaagcta ttatcgtgct gctgatggtc gtgacttcca acgctgacag gatttgcaca 60

ggcattacct ccagtaactc cccacatgtg gtgaagacag ccacccaggg cgaagtgaat 120

gtgaccggcg tgatccctct gaccacaacc ccaacaaagt cctacttcgc caatctgaag 180

ggcacacgga ccagaggcaa gctgtgccct gactgtctga actgcaccga cctggatgtg 240

gccctgggca ggcctatgtg cgtgggaaca accccatccg ccaaggcctc tatcctgcac 300

gaggtgcgcc ccgtgacctc cggatgcttt cctatcatgc acgacaggac aaagatccgc 360

cagctgccaa atctgctgcg gggctacgag aagatcagac tgtctacaca gaacgtgatc 420

gatgcagaga aggcaccagg aggaccatat cggctgggca catccggctc ttgtcccaat 480

gccaccagca agatcggctt ctttgccaca atggcctggg ccgtgcctaa ggacaactac 540

aagaatgcca caaaccccct gaccgtggag gtgccttata tctgcacaga gggcgaggac 600

cagatcaccg tgtggggctt ccacagcgat aacaagaccc agatgaagag cctgtacggc 660

gattccaatc cccagaagtt cacaagctcc gccaacggcg tgacaaccca ctatgtgagc 720

cagatcggcg actttccaga tcagaccgag gacggaggcc tgccacagtc cggcaggatc 780

gtggtggatt acatgatgca gaagcctggc aagacaggca ccatcgtgta tcagcggggc 840

gtgctgctgc cacagaaagt gtggtgtgcc tctggccgga gcaaagtgat caagggctct 900

ctgccactga tcggcgaggc cgactgcctg cacgaggagt atggcggcct gaataagagc 960

aagccctact ataccggcaa gcacgccaag gccatcggca attgtccaat ctgggtgaag 1020

acccccctga agctggccaa cggcacaaag tacaggccac ctgcaaagct gctgaaggag 1080

aggggcttct ttggagcaat cgcaggcttt ctggagggag gatgggaggg aatgatcgca 1140

ggatggcacg gatatacctc ccacggagca cacggagtgg cagtggcagc cgacctgaag 1200

tctacccagg aggccatcaa caagatcaca aagaacctga atagcctgtc cgagctggag 1260

gtgaagaatc tgcagcggct gtccggcgcc atggatgagc tgcacaacga gatcctggag 1320

ctggacgaga aggtggacga tctgagagcc gataccatct ctagccagat cgagctggcc 1380

gtgctgctgt ctaatgaggg catcatcaac agcgaggatg agcacctgct ggccctggag 1440

aggaagctga agaagatgct gggcccttct gccgtggaca tcggcaatgg ctgcttcgaa 1500

accaagcaca agtgcaacca gacatgtctg gacagaatcg ccgccggcac cttcaatgcc 1560

ggcgagtttt ctctgcccac cttcgatagc ctgaacatca cagcagccag cctgtccgcc 1620

gaggaggcag agctggccta cctgctgggc gagctggcct acaagctggg cgagtatagg 1680

atcgccatca gggcatacag gatcgccctg aagcgcgacc ccaacaatgc cgaggcctgg 1740

tacaacctgg gcaatgccta ctataagcag ggccggtata gagaggccat cgagtactat 1800

cagaaggccc tggagctgga ccccaataac gctgaagctt ggtataacct gggcaacgct 1860

tactatgagc ggggcgagta tgaggaagct atcgagtact atcggaaggc cctgagactg 1920

gacccaaaca atgccgatgc catgcagaac ctgctgaatg caaagatgag ggaggag 1977

<210> 135

<211> 659

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 135

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr

50 55 60

Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys

130 135 140

Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro

180 185 190

Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His

195 200 205

Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro

210 215 220

Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser

225 230 235 240

Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln

245 250 255

Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr

260 265 270

Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp

275 280 285

Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile

290 295 300

Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser

305 310 315 320

Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro

325 330 335

Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg

340 345 350

Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala

355 360 365

Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly

370 375 380

Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys

385 390 395 400

Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu

405 410 415

Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp

420 425 430

Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu

435 440 445

Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser

450 455 460

Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu

465 470 475 480

Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn

485 490 495

Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg

500 505 510

Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe

515 520 525

Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Ser Ala Glu Glu Ala Glu

530 535 540

Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg

545 550 555 560

Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn

565 570 575

Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg

580 585 590

Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro

595 600 605

Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg

610 615 620

Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu

625 630 635 640

Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met

645 650 655

Arg Glu Glu

<210> 136

<211> 1911

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 136

atggctatta tctacctgat cctgctgttt accgccgtga gaggggacca gatttgcatc 60

ggataccacg ctaacaactc cactgaaaag gtggacacaa tcctggagcg gaacgtgaca 120

gtgacccacg ccaaggatat cctggagaag acccacaacg gcaagctgtg caagctgaat 180

ggcatccccc ctctggagct gggcgactgc agcatcgcag gatggctgct gggcaatcca 240

gagtgtgata ggctgctgtc tgtgcccgag tggagctaca tcatggagaa ggagaacccc 300

agagacggcc tgtgcttccc tggctccttt aatgattatg aggagctgaa gcacctgctg 360

agctccgtga agcactttga gaaggtgaag atcctgccta aggacaggtg gacccagcac 420

accacaaccg gaggctccag agcatgtgcc gtgtccggca acccttcttt ctttaggaat 480

atggtgtggc tgacagagaa gggctctaac tacccagtgg ccaagggctc ctataacaat 540

acctctggcg agcagatgct gatcatctgg ggcgtgcacc accccaacga cgagaaggag 600

cagcgcacac tgtaccagaa tgtgggcacc tatgtgagcg tgggcacatc caccctgaac 660

aagcggagca caccagatat cgccaccaga cccaaagtga atggcctggg cagccggatg 720

gagttctcct ggaccctgct ggacatgtgg gatacaatca actttgagtc caccggcaat 780

ctgatcgccc cagagtacgg cttcaagatc agcaagagag gctctagcgg catcatgaag 840

acagagggca ccctggagaa ctgcgagaca aagtgtcaga cccccctggg cgccatcaac 900

acaaccctgc cttttcacaa tgtgcaccca ctgacaatcg gcgagtgccc caagtatgtg 960

aagtctgaga agctggtgct ggcaaccggc ctgaggaacg tgcctcagat cgagagccgc 1020

ggcctgttcg gagcaatcgc aggctttatc gagggaggat ggcagggaat gatcgacgga 1080

tggtacggct atcaccactc taatgaccag ggcagcggct acgccgccga taaggagagc 1140

acacagaagg ccttcgatgg catcaccaac aaggtcaatt ccgtgatcga gaagatgaac 1200

acacagttcg aggccgtggg caaggagttt tccaatctgg agcggagact ggagaacctg 1260

aataagaaga tggaggacgg cttcctggac gtgtggacat ataacgccga gctgctggtg 1320

ctgatggaga atgagaggac cctggacttc cacgattcta acgtgaagaa tctgtacgac 1380

aaggtgagga tgcagctgcg cgataacgtg aaggagctgg gcaatggctg tttcgaattc 1440

taccacaagt gcgacgatga gtgtatgaac tctgtgaaga atggcaccta cgactatccc 1500

aagtacgagg aggagagcaa gctgaacaga aatgagatca agggcgtgtc cgccgaggag 1560

gcagagctgg cctacctgct gggcgagctg gcctacaagc tgggcgagta taggatcgcc 1620

atcagggcct atcgcatcgc cctgaagcgc gaccccaaca atgccgaggc ctggtacaac 1680

ctgggcaatg cctactataa gcagggccgg tatagagagg ccatcgagta ctatcagaag 1740

gccctggagc tggaccccaa taacgctgaa gcttggtata acctgggcaa cgcttactat 1800

gagcggggcg agtatgagga agctatcgag tactatcgga aggccctgag actggaccca 1860

aacaatgccg atgccatgca gaacctgctg aatgcaaaga tgagggagga g 1911

<210> 137

<211> 637

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 137

Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Thr Ala Val Arg Gly Asp

1 5 10 15

Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp

20 25 30

Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu

35 40 45

Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro

50 55 60

Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro

65 70 75 80

Glu Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu

85 90 95

Lys Glu Asn Pro Arg Asp Gly Leu Cys Phe Pro Gly Ser Phe Asn Asp

100 105 110

Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys

115 120 125

Val Lys Ile Leu Pro Lys Asp Arg Trp Thr Gln His Thr Thr Thr Gly

130 135 140

Gly Ser Arg Ala Cys Ala Val Ser Gly Asn Pro Ser Phe Phe Arg Asn

145 150 155 160

Met Val Trp Leu Thr Glu Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly

165 170 175

Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val

180 185 190

His His Pro Asn Asp Glu Lys Glu Gln Arg Thr Leu Tyr Gln Asn Val

195 200 205

Gly Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Lys Arg Ser Thr

210 215 220

Pro Asp Ile Ala Thr Arg Pro Lys Val Asn Gly Leu Gly Ser Arg Met

225 230 235 240

Glu Phe Ser Trp Thr Leu Leu Asp Met Trp Asp Thr Ile Asn Phe Glu

245 250 255

Ser Thr Gly Asn Leu Ile Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys

260 265 270

Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys

275 280 285

Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro

290 295 300

Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val

305 310 315 320

Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val Pro Gln

325 330 335

Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly

340 345 350

Gly Trp Gln Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His Ser Asn

355 360 365

Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala

370 375 380

Phe Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn

385 390 395 400

Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg

405 410 415

Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp

420 425 430

Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu

435 440 445

Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Met

450 455 460

Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe

465 470 475 480

Tyr His Lys Cys Asp Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr

485 490 495

Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Asn Arg Asn Glu

500 505 510

Ile Lys Gly Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly

515 520 525

Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr

530 535 540

Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn

545 550 555 560

Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu

565 570 575

Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp

580 585 590

Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala

595 600 605

Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp

610 615 620

Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 138

<211> 1917

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 138

atggaaaaga ttgtgctgct gctggctatc gtgtcactgg tcaagtcaga tcagatttgt 60

atcgggtatc acgctaataa ttcaaccgag caggtggaca ccatcatgga gaagaacgtg 120

accgtgacac acgcccagga tatcctggag aagacacaca atggcaagct gtgcgacctg 180

gatggcgtga agcccctgat cctgagggac tgctctgtgg caggatggct gctgggcaac 240

ccaatgtgcg atgagttcat caatgtgcct gagtggagct acatcgtgga gaaggccaac 300

cctaccaatg acctgtgctt cccaggctcc tttaacgatt atgaggagct gaagcacctg 360

ctgtctcgga tcaatcactt tgagaagatc cagatcatcc caaagagctc ctggagcgac 420

cacgaggcat ctagcggcgt gtcctctgcc tgtccctacc tgggctctcc tagcttcttt 480

agaaacgtgg tgtggctgat caagaagaat tctacctacc ccacaatcaa gaagagctat 540

aacaatacca accaggagga cctgctggtg ctgtggggca tccaccaccc aaacgatgcc 600

gccgagcaga caaggctgta ccagaatccc accacatata tctccatcgg cacctctaca 660

ctgaaccaga ggctggtgcc taagatcgcc acccgcagca aggtcaatgg ccagtccggc 720

cgcatggagt tcttttggac aatcctgaag cctaacgacg ccatcaattt cgagagcaac 780

ggcaacttca tcgccccaga gtacgcctat aagatcgtga agaagggcga tagcgccatc 840

atgaagtccg agctggagta cggcaactgc aataccaagt gtcagacacc aatgggcgcc 900

atcaacagct ccatgccatt ccacaatatc caccccctga ccatcggcga gtgccccaag 960

tatgtgaagt ccaaccggct ggtgctggcc acaggcctga gaaattctcc tcagagggag 1020

agccgcggcc tgttcggagc aatcgcaggc tttatcgagg gaggatggca gggaatggtg 1080

gacggctggt acggctatca ccactccaac gagcagggct ctggatacgc agcagacaag 1140

gagagcaccc agaaggccat cgatggcgtg acaaacaagg tcaattccat catcgataag 1200

atgaacaccc agttcgaggc cgtgggcagg gagtttaaca atctggagcg gagaatcgag 1260

aacctgaata agaagatgga ggacggcttc ctggacgtgt ggacctataa cgccgagctg 1320

ctggtgctga tggagaatga gcgcacactg gactttcacg attccaacgt gaagaatctg 1380

tacgacaagg tgcggctgca gctgagagat aacgccaagg agctgggcaa tggctgtttc 1440

gaattctacc acaagtgcga caacgagtgt atggagtcta tccggaacgg cacctacaat 1500

tatccccagt acagcgagga ggccaggctg aagagggagg agatcagcgg cgtgtccgcc 1560

gaggaggcag agctggccta cctgctgggc gagctggcct acaagctggg cgagtatcgg 1620

atcgccatcc gggcctatag aatcgccctg aagcgggacc ccaacaatgc cgaggcctgg 1680

tacaacctgg gcaatgccta ctataagcag ggccggtata gagaggccat cgagtactat 1740

cagaaggccc tggagctgga ccccaataac gctgaagctt ggtataacct gggcaacgct 1800

tactatgaga ggggcgagta tgaggaagct atcgagtact ataggaaggc cctgcgcctg 1860

gacccaaaca atgccgatgc catgcagaac ctgctgaatg caaagatgag ggaggag 1917

<210> 139

<211> 639

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 139

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser

1 5 10 15

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val

20 25 30

Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile

35 40 45

Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys

50 55 60

Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn

65 70 75 80

Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val

85 90 95

Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Phe Pro Gly Ser Phe Asn

100 105 110

Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu

115 120 125

Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser

130 135 140

Ser Gly Val Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe

145 150 155 160

Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile

165 170 175

Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp

180 185 190

Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln

195 200 205

Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg

210 215 220

Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly

225 230 235 240

Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn

245 250 255

Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile

260 265 270

Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly

275 280 285

Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser

290 295 300

Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys

305 310 315 320

Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser

325 330 335

Pro Gln Arg Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile

340 345 350

Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His

355 360 365

Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln

370 375 380

Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys

385 390 395 400

Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu

405 410 415

Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp

420 425 430

Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg

435 440 445

Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val

450 455 460

Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe

465 470 475 480

Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn

485 490 495

Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg

500 505 510

Glu Glu Ile Ser Gly Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu

515 520 525

Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg

530 535 540

Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp

545 550 555 560

Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala

565 570 575

Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu

580 585 590

Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu

595 600 605

Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn

610 615 620

Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 140

<211> 1926

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 140

atgattgcta tcgtcgtcat tgctattctg gcatccgctg gaaagtcaga caagatttgc 60

attgggtatc acgctaacaa ctcaacaaca caggtggaca ctctgctgga gaagaacgtg 120

actgtcaccc actccgtcga gctgctggaa aaccagaagg aaaaacggtt ctgcaaaatc 180

atgaataagg cccctctgga cctgaaagat tgcaccatcg agggatggat tctgggcaac 240

ccaaagtgtg acctgctgct gggcgatcag agctggtcct acattgtgga gcggcccaac 300

gctcagaatg ggatctgctt cccaggagtc ctgaatgaac tggaggaact gaaagcattc 360

attggatctg gcgagcgagt ggaacggttc gagatgtttc ccaagagcac atgggcagga 420

gtggatactt cccgcggggt caccaacgct tgtcctagtt acactattga cagctccttt 480

tatcgaaatc tggtgtggat cgtcaaaacc gattcagcca cataccctgt gattaaggga 540

acctataaca atacaggcac tcagcccatc ctgtacttct ggggcgtgca ccatcctctg 600

gacaccacag tccaggataa cctgtacggg tctggagaca aatatgtgcg gatgggaact 660

gagtccatga actttgcaaa gtctcccgaa atcgccgcta gacctgccgt gaatggccag 720

aggagtcgca ttgattacta ttggtcagtg ctgaggccag gcgaaaccct gaatgtcgag 780

agtaacggga atctgatcgc cccctggtac gcttataaat tcgtgtcaac caacaagaaa 840

ggggcagtct ttaagagcga cctgccaatt gagaattgcg atgccacatg tcagaccatc 900

acaggagtgc tgcgcactaa caagaccttc cagaatgtct ccccactgtg gattggcgag 960

tgccccaagt acgtgaaatc tgaaagtctg agactggcta ccgggctgag gaacgtgccc 1020

cagatcgcaa cacgagggat cttcggagct attgcagggt ttatcgaggg cgggtggacc 1080

ggaatgattg acggctggta cgggtatcac catgagaaca gccagggcag cggatatgca 1140

gccgaccgag aatccacaca gaaggctatc gatggcatta ctaacaaagt gaacagcatc 1200

attaacaaga tgaatacaca gttcgaggcc gtggatcatg agtttagcaa cctggaacgg 1260

agaatcggaa acctgaacaa gaggatggag gacggcttcc tggacgtgtg gacttacaac 1320

gctgaactgc tggtcctgct ggagaatgaa aggaccctgg acctgcacga tgcaaacgtg 1380

aagaatctgt atgagaaggt caaaagccag ctgcgcgaca acgccaatga tctgggaaac 1440

ggctgtttcg aattctggca caagtgcgac aacgagtgta tggagtctgt gaagaatggc 1500

acctacgatt atcccaagta ccagaaggag agcaagctga acagacaggg aatcgagagc 1560

gtgtccgccg aggaggcaga gctggcctac ctgctgggcg agctggccta caagctgggc 1620

gagtatagga tcgccatcag ggcctatcgc atcgccctga agcgcgaccc caacaatgcc 1680

gaggcctggt acaacctggg caatgcctac tataagcagg gccggtatag agaggccatc 1740

gagtactatc agaaggccct ggagctggac cccaataacg ctgaagcttg gtataacctg 1800

ggcaacgctt actatgagcg gggcgagtat gaggaagcta tcgagtacta tcggaaggcc 1860

ctgagactgg acccaaacaa tgccgatgcc atgcagaacc tgctgaatgc aaagatgagg 1920

gaggag 1926

<210> 141

<211> 642

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 141

Met Ile Ala Ile Val Val Ile Ala Ile Leu Ala Ser Ala Gly Lys Ser

1 5 10 15

Asp Lys Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Thr Gln Val

20 25 30

Asp Thr Leu Leu Glu Lys Asn Val Thr Val Thr His Ser Val Glu Leu

35 40 45

Leu Glu Asn Gln Lys Glu Lys Arg Phe Cys Lys Ile Met Asn Lys Ala

50 55 60

Pro Leu Asp Leu Lys Asp Cys Thr Ile Glu Gly Trp Ile Leu Gly Asn

65 70 75 80

Pro Lys Cys Asp Leu Leu Leu Gly Asp Gln Ser Trp Ser Tyr Ile Val

85 90 95

Glu Arg Pro Asn Ala Gln Asn Gly Ile Cys Phe Pro Gly Val Leu Asn

100 105 110

Glu Leu Glu Glu Leu Lys Ala Phe Ile Gly Ser Gly Glu Arg Val Glu

115 120 125

Arg Phe Glu Met Phe Pro Lys Ser Thr Trp Ala Gly Val Asp Thr Ser

130 135 140

Arg Gly Val Thr Asn Ala Cys Pro Ser Tyr Thr Ile Asp Ser Ser Phe

145 150 155 160

Tyr Arg Asn Leu Val Trp Ile Val Lys Thr Asp Ser Ala Thr Tyr Pro

165 170 175

Val Ile Lys Gly Thr Tyr Asn Asn Thr Gly Thr Gln Pro Ile Leu Tyr

180 185 190

Phe Trp Gly Val His His Pro Leu Asp Thr Thr Val Gln Asp Asn Leu

195 200 205

Tyr Gly Ser Gly Asp Lys Tyr Val Arg Met Gly Thr Glu Ser Met Asn

210 215 220

Phe Ala Lys Ser Pro Glu Ile Ala Ala Arg Pro Ala Val Asn Gly Gln

225 230 235 240

Arg Ser Arg Ile Asp Tyr Tyr Trp Ser Val Leu Arg Pro Gly Glu Thr

245 250 255

Leu Asn Val Glu Ser Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Tyr

260 265 270

Lys Phe Val Ser Thr Asn Lys Lys Gly Ala Val Phe Lys Ser Asp Leu

275 280 285

Pro Ile Glu Asn Cys Asp Ala Thr Cys Gln Thr Ile Thr Gly Val Leu

290 295 300

Arg Thr Asn Lys Thr Phe Gln Asn Val Ser Pro Leu Trp Ile Gly Glu

305 310 315 320

Cys Pro Lys Tyr Val Lys Ser Glu Ser Leu Arg Leu Ala Thr Gly Leu

325 330 335

Arg Asn Val Pro Gln Ile Ala Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly

355 360 365

Tyr His His Glu Asn Ser Gln Gly Ser Gly Tyr Ala Ala Asp Arg Glu

370 375 380

Ser Thr Gln Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Ile

385 390 395 400

Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val Asp His Glu Phe Ser

405 410 415

Asn Leu Glu Arg Arg Ile Gly Asn Leu Asn Lys Arg Met Glu Asp Gly

420 425 430

Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu

435 440 445

Asn Glu Arg Thr Leu Asp Leu His Asp Ala Asn Val Lys Asn Leu Tyr

450 455 460

Glu Lys Val Lys Ser Gln Leu Arg Asp Asn Ala Asn Asp Leu Gly Asn

465 470 475 480

Gly Cys Phe Glu Phe Trp His Lys Cys Asp Asn Glu Cys Met Glu Ser

485 490 495

Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Gln Lys Glu Ser Lys

500 505 510

Leu Asn Arg Gln Gly Ile Glu Ser Val Ser Ala Glu Glu Ala Glu Leu

515 520 525

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

530 535 540

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala

545 550 555 560

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr

565 570 575

Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn

580 585 590

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly

595 600 605

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

610 615 620

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg

625 630 635 640

Glu Glu

<210> 142

<211> 1908

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 142

atgaacacac agatcctggt cttcgcactg attgctatta ttccaactaa cgccgacaag 60

atttgcctgg gacatcacgc cgtctccaac ggcaccaagg tcaataccct gacagagcga 120

ggggtggaag tggtcaacgc cactgagaca gtggaacgaa ctaatatccc ccggatttgc 180

agcaagggaa aacggaccgt ggatctgggc cagtgtgggc tgctgggaac aatcactggc 240

ccccctcagt gcgatcagtt cctggagttt tccgccgacc tgatcattga gcggagagaa 300

ggatctgatg tctgtttccc tggcaagttc gtgaacgagg aagctctgag gcagatcctg 360

cgagagagcg gaggaattga caaagaagca atgggcttca cctactccgg catcagaact 420

aatggggcca cctcagcttg caggcgcagc ggcagctcct tctatgccga gatgaagtgg 480

ctgctgtcca acacagacaa tgccgctttt ccccagatga ctaagtctta taaaaacacc 540

aggaaaagtc ctgcactgat cgtctggggc attcaccatt ccgtgtctac agccgaacag 600

actaagctgt acgggtctgg aaacaaactg gtgaccgtcg ggtctagtaa ttatcagcag 660

agcttcgtcc catcccccgg agcaaggcca caggtgaacg gcctgagcgg gcgcatcgac 720

tttcactggc tgatgctgaa ccctaatgac accgtgacat tcagttttaa tggcgcattc 780

atcgccccag atagagcctc atttctgagg ggcaagtcca tggggattca gtctggagtg 840

caggtcgatg ctaactgcga gggcgactgt taccatagtg gaggcactat catttcaaac 900

ctgccattcc agaatatcga ctctagggca gtcggcaagt gtccccgcta tgtgaaacag 960

cgaagtctgc tgctggccac agggatgaag aatgtgcctg agatcccaaa aggcagaggg 1020

ctgttcggcg ctatcgcagg gtttattgag aacggatggg aaggcctgat tgatgggtgg 1080

tacggattca ggcaccagaa tgcccaggga gagggcacag cagccgatta taagagtact 1140

cagtcagcta tcgaccagat taccgggaag ctgaaccgcc tgatcgaaaa aacaaatcag 1200

cagttcgagc tgattgataa cgagttcaac gaggtcgaaa aacagatcgg caacgtgatt 1260

aattggacca gagacagcat cacagaggtc tggtcctaca acgctgagct gctggtggca 1320

atggaaaatc agcataccat tgatctggca gacagcgaga tggataagct gtatgaacga 1380

gtgaaacggc agctgagaga gaacgctgag gaagacggga caggatgctt cgaaatcttc 1440

cacaagtgcg acgatgactg tatggccagc atccggaaca atacctacga tcactccaag 1500

tatagggagg aggccatgca gaaccgcatc cagatcgacc ccgtgtctgc cgaggaggca 1560

gagctggcct acctgctggg cgagctggcc tacaagctgg gcgagtatcg gatcgccatc 1620

agggcctacc gcatcgccct gaagagagat cccaacaatg ccgaggcctg gtacaacctg 1680

ggcaatgcct actataagca gggccggtat agagaggcca tcgagtacta tcagaaggcc 1740

ctggagctgg accctaataa cgctgaagct tggtataacc tgggcaacgc ttactatgag 1800

aggggcgagt atgaggaagc tatcgagtac tatcggaagg ccctgcggct ggaccccaac 1860

aatgccgacg ccatgcagaa cctgctgaat gcaaagatga gggaggag 1908

<210> 143

<211> 636

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 143

Met Asn Thr Gln Ile Leu Val Phe Ala Leu Ile Ala Ile Ile Pro Thr

1 5 10 15

Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr

20 25 30

Lys Val Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr

35 40 45

Glu Thr Val Glu Arg Thr Asn Ile Pro Arg Ile Cys Ser Lys Gly Lys

50 55 60

Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly

65 70 75 80

Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile

85 90 95

Glu Arg Arg Glu Gly Ser Asp Val Cys Phe Pro Gly Lys Phe Val Asn

100 105 110

Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys

115 120 125

Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr

130 135 140

Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp

145 150 155 160

Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser

165 170 175

Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu Ile Val Trp Gly Ile His

180 185 190

His Ser Val Ser Thr Ala Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn

195 200 205

Lys Leu Val Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Val Pro

210 215 220

Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Leu Ser Gly Arg Ile Asp

225 230 235 240

Phe His Trp Leu Met Leu Asn Pro Asn Asp Thr Val Thr Phe Ser Phe

245 250 255

Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys

260 265 270

Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly

275 280 285

Asp Cys Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln

290 295 300

Asn Ile Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln

305 310 315 320

Arg Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro

325 330 335

Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly

340 345 350

Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala

355 360 365

Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile

370 375 380

Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln

385 390 395 400

Gln Phe Glu Leu Ile Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile

405 410 415

Gly Asn Val Ile Asn Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser

420 425 430

Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp

435 440 445

Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg Val Lys Arg Gln

450 455 460

Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe

465 470 475 480

His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr

485 490 495

Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile

500 505 510

Asp Pro Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly Glu

515 520 525

Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr Arg

530 535 540

Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu

545 550 555 560

Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu Tyr

565 570 575

Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp Tyr

580 585 590

Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala Ile

595 600 605

Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp Ala

610 615 620

Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 144

<211> 1914

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 144

atgaacacac agatcctggt cttcgcactg attgctatta ttccaactaa cgccgacaag 60

atttgcctgg gacatcacgc cgtctccaac ggcaccaagg tcaataccct gacagagcga 120

ggggtggaag tggtcaacgc cactgagaca gtggaacgaa ctaatatccc ccggatttgc 180

agcaagggaa aacggaccgt ggatctgggc cagtgtgggc tgctgggaac aatcactggc 240

ccccctcagt gcgatcagtt cctggagttt tccgccgacc tgatcattga gcggagagaa 300

ggatctgatg tctgtttccc tggcaagttc gtgaacgagg aagctctgag gcagatcctg 360

cgagagagcg gaggaattga caaagaagca atgggcttca cctactccgg catcagaact 420

aatggggcca cctcagcttg caggcgcagc ggcagctcct tctatgccga gatgaagtgg 480

ctgctgtcca acacagacaa tgccgctttt ccccagatga ctaagtctta taaaaacacc 540

aggaaaagtc ctgcactgat cgtctggggc attcaccatt ccgtgtctac agccgaacag 600

actaagctgt acgggtctgg aaacaaactg gtgaccgtcg ggtctagtaa ttatcagcag 660

agcttcgtcc catcccccgg agcaaggcca caggtgaacg gcctgagcgg gcgcatcgac 720

tttcactggc tgatgctgaa ccctaatgac accgtgacat tcagttttaa tggcgcattc 780

atcgccccag atagagcctc atttctgagg ggcaagtcca tggggattca gtctggagtg 840

caggtcgatg ctaactgcga gggcgactgt taccatagtg gaggcactat catttcaaac 900

ctgccattcc agaatatcga ctctagggca gtcggcaagt gtccccgcta tgtgaaacag 960

cgaagtctgc tgctggccac agggatgaag aatgtgcctg agatcccaaa aggcagaggg 1020

ctgttcggcg ctatcgcagg gtttattgag aacggatggg aaggcctgat tgatgggtgg 1080

tacggattca ggcaccagaa tgcccaggga gagggcacag cagccgatta taagagtact 1140

cagtcagcta tcgaccagat taccgggaag ctgaaccgcc tgatcgaaaa aacaaatcag 1200

cagttcgagc tgattgataa cgagttcaac gaggtcgaaa aacagatcgg caacgtgatt 1260

aattggacca gagacagcat cacagaggtc tggtcctaca acgctgagct gctggtggca 1320

atggaaaatc agcataccat tgatctggca gacagcgaga tggataagct gtatgaacga 1380

gtgaaacggc agctgagaga gaacgctgag gaagacggga caggatgctt cgaaatcttc 1440

cacaagtgcg acgatgactg tatggccagc atccggaaca atacctacga tcactccaag 1500

tatagggagg aggccatgca gaaccgcatc cagatcgacc ccgtgggcgg ctctgccgag 1560

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtatcggatc 1620

gccatcaggg cctaccgcat cgccctgaag agagatccca acaatgccga ggcctggtac 1680

aacctgggca atgcctacta taagcagggc cggtatagag aggccatcga gtactatcag 1740

aaggccctgg agctggaccc taataacgct gaagcttggt ataacctggg caacgcttac 1800

tatgagaggg gcgagtatga ggaagctatc gagtactatc ggaaggccct gcggctggac 1860

cccaacaatg ccgacgccat gcagaacctg ctgaatgcaa agatgaggga ggag 1914

<210> 145

<211> 638

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 145

Met Asn Thr Gln Ile Leu Val Phe Ala Leu Ile Ala Ile Ile Pro Thr

1 5 10 15

Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr

20 25 30

Lys Val Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr

35 40 45

Glu Thr Val Glu Arg Thr Asn Ile Pro Arg Ile Cys Ser Lys Gly Lys

50 55 60

Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly

65 70 75 80

Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile

85 90 95

Glu Arg Arg Glu Gly Ser Asp Val Cys Phe Pro Gly Lys Phe Val Asn

100 105 110

Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys

115 120 125

Glu Ala Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr

130 135 140

Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp

145 150 155 160

Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser

165 170 175

Tyr Lys Asn Thr Arg Lys Ser Pro Ala Leu Ile Val Trp Gly Ile His

180 185 190

His Ser Val Ser Thr Ala Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn

195 200 205

Lys Leu Val Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Val Pro

210 215 220

Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Leu Ser Gly Arg Ile Asp

225 230 235 240

Phe His Trp Leu Met Leu Asn Pro Asn Asp Thr Val Thr Phe Ser Phe

245 250 255

Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys

260 265 270

Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly

275 280 285

Asp Cys Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln

290 295 300

Asn Ile Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln

305 310 315 320

Arg Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro

325 330 335

Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly

340 345 350

Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala

355 360 365

Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile

370 375 380

Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln

385 390 395 400

Gln Phe Glu Leu Ile Asp Asn Glu Phe Asn Glu Val Glu Lys Gln Ile

405 410 415

Gly Asn Val Ile Asn Trp Thr Arg Asp Ser Ile Thr Glu Val Trp Ser

420 425 430

Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp

435 440 445

Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg Val Lys Arg Gln

450 455 460

Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe

465 470 475 480

His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr

485 490 495

Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile

500 505 510

Asp Pro Val Gly Gly Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu

515 520 525

Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala

530 535 540

Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr

545 550 555 560

Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile

565 570 575

Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala

580 585 590

Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu

595 600 605

Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala

610 615 620

Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 146

<211> 1905

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 146

atggagacta tttcactgat gactatcctg ctgattacta ccgcctcaaa cgctgacaag 60

atttgcattg ggcaccagag cactaacagc acagagacag tggacacact gaccgagaca 120

aacgtgccag tgacacacgc caaggagctg ctgcagaccg agcacaacgg catgctgtgc 180

gccaccaatc tgggccaccc actgatcctg gacacctgca caatcgaggg cctgatctac 240

ggcaacccct cctgtgatct gctgctggag ggcagggagt ggtcctatat cgtggagcgc 300

ccatctgccg tgaatggcac atgcttcccc ggcaacgtgg agaatctgga ggagctgcgc 360

accctgttca gctccgcctc tagctaccag agaatccaga ttttccctga caccacatgg 420

aacgtgacct acacaggcac ctcccggtct tgtagcggct ccttctatag gagcatgcgc 480

tggctgacac agaagtccgg cttttacccc gtgcaggatg ccaagtatac caacaataga 540

ggcaagaata tcctgttcgt gtggggcatc caccaccctc acacatacac cgaccagacc 600

acactgtata tcaggaacga taccacaacc tctgtgacaa ccgaggagct gaatcggatc 660

tttaagcccg tgatcgtgcc tagactgctg gtgaacggcc agcagggcag gatcgactac 720

tattggagcg tgctgaagcc aggacagaca ctgcgggtga gatccaacgg caatctgatc 780

gccccttggt acggacacgt gctgtctgga ggcagccacg gacgcatcct gaagaccgat 840

ctgaagtccg gcaactgcgt ggtgcagtgt cagacagaga agggcggcct gaactctacc 900

ctgcctttcc acaatatcag caagtacgcc tttggcacct gcccaaagta tgtgaaagtg 960

aattctctga agctggcaat cggcctgcgg aacgtgcctg cacggagcaa tagaggcctg 1020

ttcggagcaa tcgcaggctt tatcgaggga ggatggccag gcctggtggc aggctggtat 1080

ggcttccagc actccaatga ccagggagtg ggaatggcag cagacagaga ttccacacag 1140

aaggccgtgg ataagatcac ctctaaggtg aacaatatcg tggacaagat gaacaagcag 1200

tacgagatca tcgatcacga gttttctgag gtggagacaa ggctgaatat gatcaacaat 1260

aagatcgacg atcagatcca ggacgtgtgg gcctataacg ccgagctgct ggtgctgctg 1320

gagaatcaga agaccctgga cgagcacgat gccaacgtga acaatctgta caataaggtg 1380

aagcgcgccc tgggcagcaa cgcaatggag gatggcaagg gctgtttcga actgtaccac 1440

aagtgcgacg atcagtgtat ggagacaatc cggaacggca catacgaccg gagaaagtat 1500

cgggaggaga gcagactgga gaggcagcgc atcgagggcg tgtccgccga ggaggcagag 1560

ctggcctacc tgctgggcga gctggcctac aagctgggcg agtatcggat cgccatcagg 1620

gcctatcgca tcgccctgaa gcgggacccc aacaatgccg aggcctggta caacctgggc 1680

aatgcctact ataagcaggg ccggtataga gaggccatcg agtactatca gaaggccctg 1740

gagctggacc ccaataacgc tgaagcttgg tataacctgg gcaacgctta ctatgagagg 1800

ggcgagtatg aggaagctat cgagtactat aggaaggccc tgcgcctgga ccccaataac 1860

gccgatgcca tgcagaacct gctgaatgca aagatgaggg aggag 1905

<210> 147

<211> 635

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 147

Met Glu Thr Ile Ser Leu Met Thr Ile Leu Leu Ile Thr Thr Ala Ser

1 5 10 15

Asn Ala Asp Lys Ile Cys Ile Gly His Gln Ser Thr Asn Ser Thr Glu

20 25 30

Thr Val Asp Thr Leu Thr Glu Thr Asn Val Pro Val Thr His Ala Lys

35 40 45

Glu Leu Leu Gln Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn Leu

50 55 60

Gly His Pro Leu Ile Leu Asp Thr Cys Thr Ile Glu Gly Leu Ile Tyr

65 70 75 80

Gly Asn Pro Ser Cys Asp Leu Leu Leu Glu Gly Arg Glu Trp Ser Tyr

85 90 95

Ile Val Glu Arg Pro Ser Ala Val Asn Gly Thr Cys Phe Pro Gly Asn

100 105 110

Val Glu Asn Leu Glu Glu Leu Arg Thr Leu Phe Ser Ser Ala Ser Ser

115 120 125

Tyr Gln Arg Ile Gln Ile Phe Pro Asp Thr Thr Trp Asn Val Thr Tyr

130 135 140

Thr Gly Thr Ser Arg Ser Cys Ser Gly Ser Phe Tyr Arg Ser Met Arg

145 150 155 160

Trp Leu Thr Gln Lys Ser Gly Phe Tyr Pro Val Gln Asp Ala Lys Tyr

165 170 175

Thr Asn Asn Arg Gly Lys Asn Ile Leu Phe Val Trp Gly Ile His His

180 185 190

Pro His Thr Tyr Thr Asp Gln Thr Thr Leu Tyr Ile Arg Asn Asp Thr

195 200 205

Thr Thr Ser Val Thr Thr Glu Glu Leu Asn Arg Ile Phe Lys Pro Val

210 215 220

Ile Val Pro Arg Leu Leu Val Asn Gly Gln Gln Gly Arg Ile Asp Tyr

225 230 235 240

Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Val Arg Ser Asn

245 250 255

Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Val Leu Ser Gly Gly Ser

260 265 270

His Gly Arg Ile Leu Lys Thr Asp Leu Lys Ser Gly Asn Cys Val Val

275 280 285

Gln Cys Gln Thr Glu Lys Gly Gly Leu Asn Ser Thr Leu Pro Phe His

290 295 300

Asn Ile Ser Lys Tyr Ala Phe Gly Thr Cys Pro Lys Tyr Val Lys Val

305 310 315 320

Asn Ser Leu Lys Leu Ala Ile Gly Leu Arg Asn Val Pro Ala Arg Ser

325 330 335

Asn Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp

340 345 350

Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser Asn Asp Gln

355 360 365

Gly Val Gly Met Ala Ala Asp Arg Asp Ser Thr Gln Lys Ala Val Asp

370 375 380

Lys Ile Thr Ser Lys Val Asn Asn Ile Val Asp Lys Met Asn Lys Gln

385 390 395 400

Tyr Glu Ile Ile Asp His Glu Phe Ser Glu Val Glu Thr Arg Leu Asn

405 410 415

Met Ile Asn Asn Lys Ile Asp Asp Gln Ile Gln Asp Val Trp Ala Tyr

420 425 430

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Gln Lys Thr Leu Asp Glu

435 440 445

His Asp Ala Asn Val Asn Asn Leu Tyr Asn Lys Val Lys Arg Ala Leu

450 455 460

Gly Ser Asn Ala Met Glu Asp Gly Lys Gly Cys Phe Glu Leu Tyr His

465 470 475 480

Lys Cys Asp Asp Gln Cys Met Glu Thr Ile Arg Asn Gly Thr Tyr Asp

485 490 495

Arg Arg Lys Tyr Arg Glu Glu Ser Arg Leu Glu Arg Gln Arg Ile Glu

500 505 510

Gly Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly Glu Leu

515 520 525

Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr Arg Ile

530 535 540

Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly

545 550 555 560

Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr

565 570 575

Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn

580 585 590

Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala Ile Glu

595 600 605

Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp Ala Met

610 615 620

Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 148

<211> 1911

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 148

atgtataaaa tcgtcgtcat cattgctctg ctgggggctg tgaaggggct ggataaaatc 60

tgtctggggc accatgctgt cgctaatggc accatcgtga agacactgac taacgagcag 120

gaggaagtga ctaatgctac cgagacagtc gaaagcaccg gcattaacag actgtgcatg 180

aaaggaagga agcacaaaga cctgggcaat tgtcatccta tcggaatgct gattggcact 240

ccagcatgcg acctgcacct gacaggcatg tgggatactc tgattgagcg cgaaaacgct 300

atcgcatact gtttccccgg ggccacagtg aatgtcgagg ctctgcgaca gaagatcatg 360

gaatctggcg ggatcaacaa aattagtacc gggtttacat acggaagctc cattaattcc 420

gcaggcacca cacgagcctg catgcggaac ggaggcaatt ctttttatgc agagctgaag 480

tggcttgtga gtaagtcaaa agggcagaac ttccctcaga ctaccaacac ctaccggaat 540

actgacaccg ccgagcatct gatcatgtgg ggcattcacc atccatctag tacacaggag 600

aagaacgatc tgtacgggac tcagagcctg tccatctctg tgggatcaag cacatataga 660

aacaatttcg tgccagtggt cggagcccga cctcaggtca acggacagtc tggccggatt 720

gactttcact ggaccctggt gcagcccggc gataacatca cattcagtca taatggggga 780

ctgattgccc ctagtcgcgt ctcaaagctg attggacgag gcctgggcat ccagagcgac 840

gctcctatcg ataacaactg cgagtcaaag tgtttttggc ggggcgggag catcaacact 900

agactgccat tccagaatct gagtcccagg accgtggggc agtgtccaaa gtacgtcaac 960

cggagatcac tgatgctggc caccggaatg agaaatgtgc ccgagctgat ccagggaagg 1020

ggcctgtttg gcgccattgc tgggttcctg gagaacgggt gggaaggaat ggtggacggc 1080

tggtacgggt ttaggcacca gaatgctcag gggaccggac aggccgctga ctataagtcc 1140

acccaggcag ccatcgatca gattacaggc aagctgaacc gcctggtgga gaaaacaaat 1200

actgagtttg aaagcatcga gtccgaattc tctgagatcg aacaccagat tggcaacgtg 1260

atcaattgga ccaaggactc tatcactgat atttggacct atcaggctga gctgctggtc 1320

gcaatggaaa accagcatac aatcgacatg gccgattccg agatgctgaa cctgtatgaa 1380

agggtgcgca agcagctgcg gcagaatgct gaggaagacg gcaaagggtg cttcgaaatc 1440

taccacgcct gcgacgatag ctgtatggag tccatccgga acaataccta cgaccactct 1500

cagtataggg aggaggccct gctgaaccgc ctgaacatca atcccgtgag cgccgaggag 1560

gcagagctgg cctacctgct gggcgagctg gcctacaagc tgggcgagta tcggatcgcc 1620

atcagggcct atcgcatcgc cctgaagcgg gaccccaaca atgccgaggc ctggtacaac 1680

ctgggcaatg cctactataa gcagggccgg tatagagagg ccatcgagta ctatcagaag 1740

gccctggagc tggaccccaa taacgctgaa gcttggtata acctgggcaa cgcttactat 1800

gagaggggcg agtatgagga agctatcgag tactatcgga aggccctgag actggaccca 1860

aacaatgccg atgccatgca gaacctgctg aatgcaaaga tgagggagga g 1911

<210> 149

<211> 637

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 149

Met Tyr Lys Ile Val Val Ile Ile Ala Leu Leu Gly Ala Val Lys Gly

1 5 10 15

Leu Asp Lys Ile Cys Leu Gly His His Ala Val Ala Asn Gly Thr Ile

20 25 30

Val Lys Thr Leu Thr Asn Glu Gln Glu Glu Val Thr Asn Ala Thr Glu

35 40 45

Thr Val Glu Ser Thr Gly Ile Asn Arg Leu Cys Met Lys Gly Arg Lys

50 55 60

His Lys Asp Leu Gly Asn Cys His Pro Ile Gly Met Leu Ile Gly Thr

65 70 75 80

Pro Ala Cys Asp Leu His Leu Thr Gly Met Trp Asp Thr Leu Ile Glu

85 90 95

Arg Glu Asn Ala Ile Ala Tyr Cys Phe Pro Gly Ala Thr Val Asn Val

100 105 110

Glu Ala Leu Arg Gln Lys Ile Met Glu Ser Gly Gly Ile Asn Lys Ile

115 120 125

Ser Thr Gly Phe Thr Tyr Gly Ser Ser Ile Asn Ser Ala Gly Thr Thr

130 135 140

Arg Ala Cys Met Arg Asn Gly Gly Asn Ser Phe Tyr Ala Glu Leu Lys

145 150 155 160

Trp Leu Val Ser Lys Ser Lys Gly Gln Asn Phe Pro Gln Thr Thr Asn

165 170 175

Thr Tyr Arg Asn Thr Asp Thr Ala Glu His Leu Ile Met Trp Gly Ile

180 185 190

His His Pro Ser Ser Thr Gln Glu Lys Asn Asp Leu Tyr Gly Thr Gln

195 200 205

Ser Leu Ser Ile Ser Val Gly Ser Ser Thr Tyr Arg Asn Asn Phe Val

210 215 220

Pro Val Val Gly Ala Arg Pro Gln Val Asn Gly Gln Ser Gly Arg Ile

225 230 235 240

Asp Phe His Trp Thr Leu Val Gln Pro Gly Asp Asn Ile Thr Phe Ser

245 250 255

His Asn Gly Gly Leu Ile Ala Pro Ser Arg Val Ser Lys Leu Ile Gly

260 265 270

Arg Gly Leu Gly Ile Gln Ser Asp Ala Pro Ile Asp Asn Asn Cys Glu

275 280 285

Ser Lys Cys Phe Trp Arg Gly Gly Ser Ile Asn Thr Arg Leu Pro Phe

290 295 300

Gln Asn Leu Ser Pro Arg Thr Val Gly Gln Cys Pro Lys Tyr Val Asn

305 310 315 320

Arg Arg Ser Leu Met Leu Ala Thr Gly Met Arg Asn Val Pro Glu Leu

325 330 335

Ile Gln Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Leu Glu Asn

340 345 350

Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn

355 360 365

Ala Gln Gly Thr Gly Gln Ala Ala Asp Tyr Lys Ser Thr Gln Ala Ala

370 375 380

Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Val Glu Lys Thr Asn

385 390 395 400

Thr Glu Phe Glu Ser Ile Glu Ser Glu Phe Ser Glu Ile Glu His Gln

405 410 415

Ile Gly Asn Val Ile Asn Trp Thr Lys Asp Ser Ile Thr Asp Ile Trp

420 425 430

Thr Tyr Gln Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile

435 440 445

Asp Met Ala Asp Ser Glu Met Leu Asn Leu Tyr Glu Arg Val Arg Lys

450 455 460

Gln Leu Arg Gln Asn Ala Glu Glu Asp Gly Lys Gly Cys Phe Glu Ile

465 470 475 480

Tyr His Ala Cys Asp Asp Ser Cys Met Glu Ser Ile Arg Asn Asn Thr

485 490 495

Tyr Asp His Ser Gln Tyr Arg Glu Glu Ala Leu Leu Asn Arg Leu Asn

500 505 510

Ile Asn Pro Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr Leu Leu Gly

515 520 525

Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile Arg Ala Tyr

530 535 540

Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala Trp Tyr Asn

545 550 555 560

Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu Ala Ile Glu

565 570 575

Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala Glu Ala Trp

580 585 590

Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr Glu Glu Ala

595 600 605

Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn Asn Ala Asp

610 615 620

Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635

<210> 150

<211> 1932

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 150

atgaaaacta ttatcgccct gtcatacatc ctgtgcctgg tctttgctca gaaaatccct 60

ggaaacgaca actccaccgc caccctgtgt ctgggccacc acgccgtgcc aaatggcacc 120

atcgtgaaga ccatcacaaa cgaccgcatc gaggtgacca atgccacaga gctggtgcag 180

aacagctcca tcggcgagat ttgcgacagc ccccaccaga tcctggatgg cgagaactgt 240

acactgatcg acgcactgct gggcgaccct cagtgcgatg gcttccagaa taagaagtgg 300

gatctgtttg tggagcgcag caaggcctac tccaactgtt tcccctacga cgtgcctgat 360

tatgcctctc tgcggagcct ggtggcatct agcggcaccc tggagttcaa gaatgagagc 420

tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gggcagctcc 480

tctagcttct tttcccggct gaactggctg acccacctga attacacata tcctgccctg 540

aatgtgacca tgccaaacaa ggagcagttc gataagctgt acatctgggg agtgcaccac 600

ccaggaacag acaaggatca gattttcctg tatgcccagt cctctggccg catcaccgtg 660

tctacaaagc ggagccagca ggccgtgatc cctaacatcg gctctaggcc acgcatccgg 720

gacatcccct ccagaatctc tatctactgg accatcgtga agccaggcga tatcctgctg 780

atcaactcca caggcaatct gatcgccccc agaggctatt tcaagatcag gtccggcaag 840

agctccatca tgaggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacccct 900

aacggctcca tcccaaatga taagcccttt cagaacgtga atcgcatcac atacggcgcc 960

tgtcctcggt acgtgaagca cagcaccctg aagctggcca caggcatgag aaacgtgcca 1020

gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080

ggcatggtgg acggctggta cggctttaga caccagaact ctgagggaag gggacaggca 1140

gccgacctga agagcaccca ggccgccatc gatcagatca acggcaagct gaatagactg 1200

atcggcaaga caaatgagaa gttccaccag atcgagaagg agtttagcga ggtggagggc 1260

agagtgcagg acctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320

gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380

aacaagctgt tcgaaaagac caagaagcag ctgagggaga acgccgagga catgggcaat 1440

ggctgcttca aaatctacca caagtgcgat aacgcctgta tcgagagcat ccgcaacgag 1500

acatacgacc acaacgtgta cagggatgag gccctgaaca atcgctttca gatcaagggc 1560

gtgggcggct ccgccgagga ggcagagctg gcctacctgc tgggcgagct ggcctacaag 1620

ctgggcgagt atcggatcgc catcagggcc tatcgcatcg ccctgaagcg ggaccccaac 1680

aatgccgagg cctggtacaa cctgggcaat gcctactata agcagggccg gtatagagag 1740

gccatcgagt actatcagaa ggccctggag ctggacccca ataacgctga agcttggtat 1800

aacctgggca acgcttacta tgagcggggc gagtatgagg aagctatcga gtactatcgg 1860

aaggccctga gactggaccc caataacgcc gatgccatgc agaacctgct gaatgccaag 1920

atgagagagg ag 1932

<210> 151

<211> 644

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 151

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala

1 5 10 15

Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly

20 25 30

His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp

35 40 45

Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile

50 55 60

Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys

65 70 75 80

Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln

85 90 95

Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn

100 105 110

Cys Phe Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val

115 120 125

Ala Ser Ser Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Thr

130 135 140

Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Gly Ser Ser

145 150 155 160

Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr

165 170 175

Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys

180 185 190

Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile

195 200 205

Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg

210 215 220

Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg

225 230 235 240

Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly

245 250 255

Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly

260 265 270

Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala

275 280 285

Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile

290 295 300

Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala

305 310 315 320

Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met

325 330 335

Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala

340 345 350

Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly

355 360 365

Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys

370 375 380

Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu

385 390 395 400

Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser

405 410 415

Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr

420 425 430

Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu

435 440 445

Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe

450 455 460

Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn

465 470 475 480

Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Glu Ser

485 490 495

Ile Arg Asn Glu Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu

500 505 510

Asn Asn Arg Phe Gln Ile Lys Gly Val Gly Gly Ser Ala Glu Glu Ala

515 520 525

Glu Leu Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr

530 535 540

Arg Ile Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn

545 550 555 560

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly

565 570 575

Arg Tyr Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp

580 585 590

Pro Asn Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu

595 600 605

Arg Gly Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg

610 615 620

Leu Asp Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys

625 630 635 640

Met Arg Glu Glu

<210> 152

<211> 1968

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 152

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa gcgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacgtgcgcc tgtctacaca caacgtgatc 420

aatgccgagg gcgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaga caagaacaag 540

acagccacca accccctgac catcgaggtg ccttacgtgt gcaccgaggg cgaggaccag 600

atcacagtgt ggggcttcca ctccgataat gagacacaga tggccaagct gtacggcgat 660

tctaagcctc agaagttcac cagctccgcc aatggcgtga caacccacta cgtgagccag 720

atcggcggct ttccaaacca gaccgaggac ggaggcctgc cacagtctgg acggatcgtg 780

gtggattaca tggtgcagaa gagcggcaag acaggcacca tcacatatca gcggggcatc 840

ctgctgcccc agaaagtgtg gtgtgcctct ggccggagca aagtgatcaa gggctctctg 900

cctctgatcg gcgaggccga ttgcctgcac gagaagtatg gcggcctgaa caagagcaag 960

ccatactata caggcgagca cgccaaggcc atcggcaatt gtccaatctg ggtgaagaca 1020

cccctgaagc tggccaacgg caccaagtac aggccacctg caaagctgct gaaggagagg 1080

ggcttctttg gagcaatcgc aggctttctg gagggaggat gggagggaat gatcgcagga 1140

tggcacggat ataccagcca cggagcacac ggagtggcag tggcagccga cctgaagtcc 1200

acacaggagg ccatcaacaa gatcaccaag aacctgaata gcctgtccga gctggaggtg 1260

aagaatctgc agcggctgag cggcgccatg gatgagctgc acaacgagat cctggagctg 1320

gacgagaagg tggacgatct gagagccgat accatctcta gccagatcga gctggccgtg 1380

ctgctgagca atgagggcat catcaactcc gaggacgagc acctgctggc cctggagagg 1440

aagctgaaga agatgctggg accatccgcc gtggagatcg gaaatggctg cttcgaaacc 1500

aagcacaagt gcaaccagac atgtctggac aaaatcgcag caggcacctt cgatgcagga 1560

gagttttctc tgcccacctt cgacagcctg aatatcacag cagcctccgc cgaggaggca 1620

gagctggcct acctgctggg cgagctggcc tacaagctgg gcgagtatcg gatcgccatc 1680

agggcctatc gcatcgccct gaagcgggac cccaacaatg ccgaggcctg gtacaacctg 1740

ggcaatgcct actataagca gggccggtat agagaggcca tcgagtacta tcagaaggcc 1800

ctggagctgg accccaataa cgctgaagct tggtataacc tgggcaacgc ttactatgag 1860

cggggcgagt atgaggaagc tatcgagtac tatcggaagg ccctgagact ggaccccaat 1920

aacgccgatg ccatgcagaa cctgctgaat gccaagatga gagaggag 1968

<210> 153

<211> 656

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 153

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Ser Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Gly

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro Tyr

180 185 190

Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His Ser

195 200 205

Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro Gln

210 215 220

Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser Gln

225 230 235 240

Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser

245 250 255

Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly

260 265 270

Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys

275 280 285

Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly

290 295 300

Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys

305 310 315 320

Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile

325 330 335

Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg Pro

340 345 350

Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly

355 360 365

Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr

370 375 380

Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser

385 390 395 400

Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu Ser

405 410 415

Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu

420 425 430

Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu Arg

435 440 445

Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser Asn

450 455 460

Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu Arg

465 470 475 480

Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn Gly

485 490 495

Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys Ile

500 505 510

Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp

515 520 525

Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu Ala Tyr

530 535 540

Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile

545 550 555 560

Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala

565 570 575

Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu

580 585 590

Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala

595 600 605

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr

610 615 620

Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn

625 630 635 640

Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

645 650 655

<210> 154

<211> 1920

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 154

atgaaagcta agctcctggt gttgttgtgc acgtttacag ctacatacgc ggacactata 60

tgtatcggct accacgcgaa taactctacc gatacggttg ataccgtcct ggagaaaaat 120

gtcactgtta cacattccgt aaacttgttg gaggattctc acaatggaaa gctgtgcctc 180

ctcaagggca ttgcaccgct ccaactcggc aactgtagtg ttgctggctg gatactcggc 240

aacccagagt gtgagttgct catcagcaaa gaaagttgga gctacatcgt cgagactccc 300

aatcccgaaa atggaacttg ctttccggga tattttgcag actacgaaga actgagggag 360

cagctgtcat ctgtctcctc ttttgagaga tttgagatct tccccaaaga aagttcttgg 420

ccaaatcaca cggtaaccgg agtgtcagcg tcatgcagcc acaatggtaa atcctccttc 480

tacaggaatc ttttgtggct cacaggtaag aatggtttgt atccaaatct ttcaaaaagc 540

tatgtcaaca acaaggaaaa ggaagtactc gtcttgtggg gagtgcacca ccctcctaac 600

ataggcaacc agagagcgct ctaccatacg gagaatgcat acgtgtcagt ggtgtccagt 660

cattactctc ggcgattcac ccctgaaatc gcaaagcggc caaaggtaag ggatcaggaa 720

ggcagaataa attactattg gactctgctc gagcccggcg acactattat ttttgaggcg 780

aatggcaacc ttattgcgcc ttggtatgct ttcgcacttt cacgcgggtt tgggtccgga 840

atcattacct ccaacgctcc catggacgaa tgcgatgcca agtgccaaac tccgcaagga 900

gctatcaata gtagtttgcc ttttcaaaat gtacatcccg tgacgatagg cgaatgtccg 960

aaatacgtaa gaagcgcaaa gctccggatg gtaactggcc ttagaaacat accctccatt 1020

cagtcaaggg gcctgttcgg agccattgcc ggttttatcg aaggtggttg gactggtatg 1080

gtggatggtt ggtacggcta tcaccaccaa aacgaacaag gaagtggtta cgcagccgat 1140

cagaagagta ctcaaaacgc aattaatggc ataaccaaca aggtcaactc agtgatcgag 1200

aaaatgaaca ctcagtttac cgcagttggt aaagaattta acaagcttga gcggaggatg 1260

gagaacctca ataaaaaagt tgacgacggg ttccttgata tctggaccta caacgctgaa 1320

cttcttgtac tgctggagaa tgagcgaaca ctggactttc acgattccaa tgtgaaaaac 1380

ctgtatgaaa aggttaagag tcaactgaag aataatgcga aggagattgg gaacggttgt 1440

ttcgagttct atcacaaatg taacaacgaa tgcatggagt ccgttaagaa cgggacatac 1500

gactatccca aatactccga ggaaagcaag cttaacaggg agaaaataga cggtgtttct 1560

gccgaggagg cagagctggc ctacctgctg ggcgagctgg cctacaagct gggcgagtat 1620

aggatcgcca tcagggccta tcgcatcgcc ctgaagcgcg accccaacaa tgccgaggcc 1680

tggtacaacc tgggcaatgc ctactataag cagggccggt atagagaggc catcgagtac 1740

tatcagaagg ccctggagct ggaccccaat aacgctgaag cttggtataa cctgggcaac 1800

gcttactatg agcggggcga gtatgaggaa gctatcgagt actatcggaa ggccctgaga 1860

ctggacccaa acaatgccga tgccatgcag aacctgctga atgcaaagat gagggaggag 1920

<210> 155

<211> 640

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 155

Met Lys Ala Lys Leu Leu Val Leu Leu Cys Thr Phe Thr Ala Thr Tyr

1 5 10 15

Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile

50 55 60

Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile

85 90 95

Val Glu Thr Pro Asn Pro Glu Asn Gly Thr Cys Phe Pro Gly Tyr Phe

100 105 110

Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr

130 135 140

Val Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Lys Ser Ser Phe

145 150 155 160

Tyr Arg Asn Leu Leu Trp Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn

165 170 175

Leu Ser Lys Ser Tyr Val Asn Asn Lys Glu Lys Glu Val Leu Val Leu

180 185 190

Trp Gly Val His His Pro Pro Asn Ile Gly Asn Gln Arg Ala Leu Tyr

195 200 205

His Thr Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg

210 215 220

Arg Phe Thr Pro Glu Ile Ala Lys Arg Pro Lys Val Arg Asp Gln Glu

225 230 235 240

Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile

245 250 255

Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Phe Ala

260 265 270

Leu Ser Arg Gly Phe Gly Ser Gly Ile Ile Thr Ser Asn Ala Pro Met

275 280 285

Asp Glu Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser

290 295 300

Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro

305 310 315 320

Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn

325 330 335

Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe

340 345 350

Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His

355 360 365

His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr

370 375 380

Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu

385 390 395 400

Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu

405 410 415

Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu

420 425 430

Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu

435 440 445

Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys

450 455 460

Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys

465 470 475 480

Phe Glu Phe Tyr His Lys Cys Asn Asn Glu Cys Met Glu Ser Val Lys

485 490 495

Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn

500 505 510

Arg Glu Lys Ile Asp Gly Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr

515 520 525

Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile

530 535 540

Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala

545 550 555 560

Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu

565 570 575

Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala

580 585 590

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr

595 600 605

Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn

610 615 620

Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635 640

<210> 156

<211> 1920

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 156

atgaaggtta aactgttggt cctcctttgc actttcaccg cgacgtatgc cgataccatc 60

tgtatcggat atcatgcaaa caattctacc gatacagtcg atacggttct tgagaagaac 120

gtgactgtga cacatagtgt gaatcttctt gaggactccc ataacgggaa gctttgtctc 180

ctcaaaggta tagcacctct tcaactgggc aattgctctg tagccggttg gatactgggt 240

aatcctgaat gtgagctcct gatatccagg gaaagctggt catatattgt agagaaacct 300

aatccagaaa atggtacctg tttcccagga catttcgcgg attatgagga actgcgcgag 360

cagctgtcca gtgtcagtag ttttgaacga ttcgaaatct ttccgaagga gagttcatgg 420

ccaaatcaca cgactacagg cgtctcagcg tcctgttcac acaacggtga gagctccttc 480

tacaaaaacc ttctgtggct gacaggcaaa aatggcctct atccaaatct ttctaagagc 540

tatgcaaaca acaaggagaa agaagttctg gtactctggg gggtacatca ccctccaaac 600

attggtgacc aaagagcttt gtaccataag gaaaatgcgt acgtctcagt agtgtccagt 660

cactactcac gaaagttcac accagaaatc gcaaagcgcc cgaaagtaag ggaccaggag 720

gggcggataa actactactg gacactcctg gaaccgggcg atacgatcat cttcgaagca 780

aacggtaatc ttatagctcc ccgatacgct ttcgcgctgt ccaggggttt cgggagtggt 840

ataataaact ccaacgcacc tatggacgaa tgtgatgcta aatgccaaac gccccagggg 900

gccattaata gtagtcttcc gtttcagaat gttcatcctg taactatcgg ggagtgccct 960

aagtacgtac gcagtgctaa gctcagaatg gtgaccggac tccgaaacat accttctatt 1020

caatctcggg gccttttcgg ggctatagcg ggcttcatag aaggaggctg gaccggcatg 1080

gtcgacgggt ggtatgggta ccaccaccag aacgagcaag gctcagggta cgcagcggat 1140

caaaagtcta cccagaatgc aattaatggt attactaaca aggtaaacag tgttattgag 1200

aaaatgaaca ctcaatttac tgcagtgggt aaagagttca acaaactcga acggcgcatg 1260

gagaatctga acaaaaaagt ggatgacgga tttatagaca tctggacata taacgcagaa 1320

ctgcttgtcc ttctcgagaa cgaaaggaca ctcgactttc acgacagcaa cgtcaaaaac 1380

ttgtatgaaa aagtcaaaag tcagttgaag aataatgcga aagaaatcgg aaacggttgt 1440

ttcgagttct atcacaagtg taatgatgaa tgtatggagt ccgtgaagaa cgggacttat 1500

gactatccta aatattctga agagtcaaag cttaatcggg aaaaaatcga cggtgtttct 1560

gccgaggagg cagagctggc ctacctgctg ggcgagctgg cctacaagct gggcgagtat 1620

aggatcgcca tcagggccta tcgcatcgcc ctgaagcgcg accccaacaa tgccgaggcc 1680

tggtacaacc tgggcaatgc ctactataag cagggccggt atagagaggc catcgagtac 1740

tatcagaagg ccctggagct ggaccccaat aacgctgaag cttggtataa cctgggcaac 1800

gcttactatg agcggggcga gtatgaggaa gctatcgagt actatcggaa ggccctgaga 1860

ctggacccaa acaatgccga tgccatgcag aacctgctga atgcaaagat gagggaggag 1920

<210> 157

<211> 640

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 157

Met Lys Val Lys Leu Leu Val Leu Leu Cys Thr Phe Thr Ala Thr Tyr

1 5 10 15

Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr

20 25 30

Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn

35 40 45

Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile

50 55 60

Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly

65 70 75 80

Asn Pro Glu Cys Glu Leu Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile

85 90 95

Val Glu Lys Pro Asn Pro Glu Asn Gly Thr Cys Phe Pro Gly His Phe

100 105 110

Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe

115 120 125

Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr

130 135 140

Thr Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Glu Ser Ser Phe

145 150 155 160

Tyr Lys Asn Leu Leu Trp Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn

165 170 175

Leu Ser Lys Ser Tyr Ala Asn Asn Lys Glu Lys Glu Val Leu Val Leu

180 185 190

Trp Gly Val His His Pro Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr

195 200 205

His Lys Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg

210 215 220

Lys Phe Thr Pro Glu Ile Ala Lys Arg Pro Lys Val Arg Asp Gln Glu

225 230 235 240

Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile

245 250 255

Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala

260 265 270

Leu Ser Arg Gly Phe Gly Ser Gly Ile Ile Asn Ser Asn Ala Pro Met

275 280 285

Asp Glu Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser

290 295 300

Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro

305 310 315 320

Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn

325 330 335

Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe

340 345 350

Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His

355 360 365

His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr

370 375 380

Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu

385 390 395 400

Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu

405 410 415

Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile

420 425 430

Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu

435 440 445

Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys

450 455 460

Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys

465 470 475 480

Phe Glu Phe Tyr His Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys

485 490 495

Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn

500 505 510

Arg Glu Lys Ile Asp Gly Val Ser Ala Glu Glu Ala Glu Leu Ala Tyr

515 520 525

Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile Ala Ile

530 535 540

Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala Glu Ala

545 550 555 560

Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr Arg Glu

565 570 575

Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asn Asn Ala

580 585 590

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly Glu Tyr

595 600 605

Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp Pro Asn

610 615 620

Asn Ala Asp Ala Met Gln Asn Leu Leu Asn Ala Lys Met Arg Glu Glu

625 630 635 640

<210> 158

<211> 1974

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 158

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc ctccgccgag 1620

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtataggatc 1680

gccatcaggg catacaggat cgccctgaag cgcgaccccg acaatgccga ggcctggtac 1740

aacctgggcg acgcctacta taaggaaggc cggtatagag aggccatcga gtactatcag 1800

aaggccctgg agctggaccc cgataacgct gaagcttggt ataacctggg cgatgcttac 1860

tatgagcggg gcgagtatga ggaagctatc gagtactatc ggaaggccct gagactggac 1920

ccaaacaatg ccgatgccat gcagaacctg ctggaagcaa agatgaggga ggag 1974

<210> 159

<211> 658

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 159

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu

530 535 540

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

545 550 555 560

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asp Asn Ala

565 570 575

Glu Ala Trp Tyr Asn Leu Gly Asp Ala Tyr Tyr Lys Glu Gly Arg Tyr

580 585 590

Arg Glu Ala Ile Glu Tyr Tyr Gln Lys Ala Leu Glu Leu Asp Pro Asp

595 600 605

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asp Ala Tyr Tyr Glu Arg Gly

610 615 620

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

625 630 635 640

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Glu Ala Lys Met Arg

645 650 655

Glu Glu

<210> 160

<211> 1974

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 160

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc ctccgccgag 1620

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtataggatc 1680

gccatcaggg catacaggat cgccctgaag cgcgaccccg acaatgccga ggcctggtac 1740

aacctgggcg acgcctacta tgaagaaggc cggtatagag aggccatcga gtactatcag 1800

gaagccctgg agctggaccc cgataacgct gaagcttggt ataacctggg cgatgcttac 1860

tatgagcggg gcgagtatga ggaagctatc gagtactatc gggaggccct gagactggac 1920

ccaaacaatg ccgatgccat gcagaacctg ctggaagcaa agatgaggga ggag 1974

<210> 161

<211> 658

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 161

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu

530 535 540

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

545 550 555 560

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asp Asn Ala

565 570 575

Glu Ala Trp Tyr Asn Leu Gly Asp Ala Tyr Tyr Glu Glu Gly Arg Tyr

580 585 590

Arg Glu Ala Ile Glu Tyr Tyr Gln Glu Ala Leu Glu Leu Asp Pro Asp

595 600 605

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asp Ala Tyr Tyr Glu Arg Gly

610 615 620

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Glu Ala Leu Arg Leu Asp

625 630 635 640

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Glu Ala Lys Met Arg

645 650 655

Glu Glu

<210> 162

<211> 1974

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 162

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc ctccgccgag 1620

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtataggatc 1680

gccatcaggg catacaggat cgccctgaag cgcgacccca acaatgccga ggcctggtac 1740

aacctgggca atgcctacta taagcagggc cggtatagag aggccatcaa atactatcgc 1800

aaggccctga aactggaccc caataacgct gaagcttggt ataacctggg caacgcttac 1860

tatgagcggg gcgagtatga ggaagctatc gagtactatc ggaaggccct gagactggac 1920

ccaaacaatg ccgatgccat gcagaacctg ctgaaagcaa agatgaggga ggag 1974

<210> 163

<211> 658

<212> PRT

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 163

Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp

1 5 10 15

Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys

20 25 30

Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr

35 40 45

Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr

50 55 60

Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val

65 70 75 80

Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val

85 90 95

Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile

100 105 110

Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly

115 120 125

Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn

130 135 140

Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn

145 150 155 160

Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro

165 170 175

Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val

180 185 190

Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe

195 200 205

His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys

210 215 220

Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val

225 230 235 240

Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro

245 250 255

Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys

260 265 270

Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val

275 280 285

Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu

290 295 300

Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys

305 310 315 320

Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys

325 330 335

Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr

340 345 350

Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile

355 360 365

Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His

370 375 380

Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu

385 390 395 400

Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser

405 410 415

Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met

420 425 430

Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp

435 440 445

Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu

450 455 460

Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu

465 470 475 480

Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly

485 490 495

Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp

500 505 510

Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr

515 520 525

Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Ala Glu Glu Ala Glu Leu

530 535 540

Ala Tyr Leu Leu Gly Glu Leu Ala Tyr Lys Leu Gly Glu Tyr Arg Ile

545 550 555 560

Ala Ile Arg Ala Tyr Arg Ile Ala Leu Lys Arg Asp Pro Asn Asn Ala

565 570 575

Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Lys Gln Gly Arg Tyr

580 585 590

Arg Glu Ala Ile Lys Tyr Tyr Arg Lys Ala Leu Lys Leu Asp Pro Asn

595 600 605

Asn Ala Glu Ala Trp Tyr Asn Leu Gly Asn Ala Tyr Tyr Glu Arg Gly

610 615 620

Glu Tyr Glu Glu Ala Ile Glu Tyr Tyr Arg Lys Ala Leu Arg Leu Asp

625 630 635 640

Pro Asn Asn Ala Asp Ala Met Gln Asn Leu Leu Lys Ala Lys Met Arg

645 650 655

Glu Glu

<210> 164

<211> 1974

<212> DNA

<213> 人工序列（Artificial Sequence）

<220>

<223> 合成序列

<400> 164

atgaaagcca ttattgtcct gctgatggtc gtcacctcaa acgctgatag aatctgcacc 60

ggcattacct cctcaaactc ccctcacgtg gtgaagacag ccacccaggg cgaggtgaac 120

gtgacaggcg tgatccctct gaccacaacc ccaaccaaga gccacttcgc caatctgaag 180

ggcacagaga caaggggcaa gctgtgccca aagtgtctga actgcacaga cctggatgtg 240

gccctgggcc gccctaagtg taccggcaag atcccatccg cccgggtgtc tatcctgcac 300

gaggtgcggc ccgtgacatc cggatgcttt cctatcatgc acgaccggac caagatcaga 360

cagctgccca atctgctgag gggctacgag cacatccgcc tgtctacaca caacgtgatc 420

aatgccgaga acgccccagg cggcccctat aagatcggca cctccggctc ttgtcctaat 480

atcacaaacg gcaatggctt ctttgccacc atggcctggg ccgtgccaaa gaatgacaag 540

aacaagacag ccaccaaccc cctgaccatc gaggtgcctt acatctgcac cgagggcgag 600

gaccagatca cagtgtgggg cttccactcc gataatgaga cacagatggc caagctgtac 660

ggcgattcta agcctcagaa gttcaccagc tccgccaatg gcgtgacaac ccactacgtg 720

agccagatcg gcggctttcc aaaccagacc gaggacggag gcctgccaca gtctggacgg 780

atcgtggtgg attacatggt gcagaagagc ggcaagacag gcaccatcac atatcagcgg 840

ggcatcctgc tgccccagaa agtgtggtgt gcctctggcc ggagcaaagt gatcaagggc 900

tctctgcctc tgatcggcga ggccgattgc ctgcacgaga agtatggcgg cctgaacaag 960

agcaagccat actatacagg cgagcacgcc aaggccatcg gcaattgtcc aatctgggtg 1020

aagacacccc tgaagctggc caacggcacc aagtacaggc cacctgcaaa gctgctgaag 1080

gagaggggct tctttggagc aatcgcaggc tttctggagg gaggatggga gggaatgatc 1140

gcaggatggc acggatatac cagccacgga gcacacggag tggcagtggc agccgacctg 1200

aagtccacac aggaggccat caacaagatc accaagaacc tgaatagcct gtccgagctg 1260

gaggtgaaga atctgcagcg gctgagcggc gccatggatg agctgcacaa cgagatcctg 1320

gagctggacg agaaggtgga cgatctgaga gccgatacca tctctagcca gatcgagctg 1380

gccgtgctgc tgagcaatga gggcatcatc aactccgagg acgagcacct gctggccctg 1440

gagaggaagc tgaagaagat gctgggacca tccgccgtgg agatcggaaa tggctgcttc 1500

gaaaccaagc acaagtgcaa ccagacatgt ctggacagaa tcgcagcagg caccttcgat 1560

gcaggagagt tttctctgcc caccttcgac agcctgaata tcacagcagc ctccgccgag 1620

gaggcagagc tggcctacct gctgggcgag ctggcctaca agctgggcga gtataggatc 1680

gccatcaggg catacaggat cgccctgaag cgcgacccca aaaatgccga ggcctggtac 1740

aacctgggca atgcctacta taagcagggc cggtatagag aggccatcaa atactatcgc 1800

aaggccctga aactggaccc caagaacgct gaagcttggt ataacctggg caacgcttac 1860

tatgagcggg gcgagtatga ggaagctatc aagtactatc ggaaggccct gagactggac 1920

ccaaacaatg ccgatgccat gcagaacctg ctgaaagcaa agatgaggga ggag 1974

<210> 165

<211> 658

<212> PRT

<213> 人工序列（Artificial Sequence）