阅读说明：本技术 一种黄豆蛋白发酵饲料及其制备方法 (Soybean protein fermented feed and preparation method thereof ) 是由 黄志雷 于 2020-05-13 设计创作，主要内容包括：本发明涉及饲料技术领域,公开了一种黄豆蛋白发酵饲料,以质量份计,该饲料包括以下组分：鲜黄豆渣25-50份、玉米粉20-30份、麸皮15-30份、玉米皮15-30份、小苏打1-3份、畜牧盐1-3份、黄豆浆10-30份、发酵源菌5-10份。本发明有效促进动物的肠胃蠕动,提高肠胃微生物的活性,使得发酵后的饲料微生物蛋白显著增加、微生物活性多糖、微生物分泌代谢物也提高明显,可以高效促进动物肠道中的益生菌高效定植,通过饲料中的营养成分增加和微生物活性的增强,显著提高动物对饲料的利用率,加速动物对于营养成分的吸收积累,进而达到提高动物肉制品中营养成分含量的目的。(The invention relates to the technical field of feed, and discloses a soybean protein fermented feed which comprises the following components in parts by mass: 25-50 parts of fresh soybean dregs, 20-30 parts of corn flour, 15-30 parts of bran, 15-30 parts of corn bran, 1-3 parts of baking soda, 1-3 parts of livestock salt, 10-30 parts of soybean milk and 5-10 parts of fermentation source bacteria. The feed additive effectively promotes the gastrointestinal peristalsis of animals, improves the activity of gastrointestinal microorganisms, obviously increases microbial protein of the fermented feed, obviously improves microbial active polysaccharide and microbial secretion metabolites, can efficiently promote the efficient field planting of probiotics in the intestinal tracts of the animals, remarkably improves the utilization rate of the animals to the feed through the increase of nutrient components in the feed and the enhancement of microbial activity, accelerates the absorption and accumulation of the nutrient components of the animals, and further achieves the purpose of improving the content of the nutrient components in animal meat products.)

1. The soybean protein fermented feed is characterized by comprising the following components in parts by mass: 25-50 parts of fresh soybean dregs, 20-30 parts of corn flour, 15-30 parts of bran, 15-30 parts of corn bran, 1-3 parts of baking soda, 1-3 parts of livestock salt, 10-30 parts of soybean milk and 5-10 parts of fermentation source bacteria.

2. The soybean protein fermented feed according to claim 1, comprising the following components in parts by mass: 33 parts of fresh soybean dregs, 29 parts of corn flour, 17 parts of bran, 19 parts of corn bran, 2 parts of baking soda, 2 parts of livestock salt, 23 parts of soybean milk and 7 parts of fermentation source bacteria.

3. The fermented soybean protein feed according to claim 1, wherein the fresh soybean dregs contain 85-88% of water and 2-3% of protein.

4. The soy protein fermented feed according to claim 1, wherein the corn flour has a fineness of 30-50 mesh and a water content of 5-10%.

5. The fermented soybean protein feed according to claim 1, wherein the corn husk has a particle size of 1 to 3 mm.

6. The soybean protein fermented feed according to claim 1, wherein the fermentation source bacteria are prepared from apple pomace, lactic acid bacteria, bacillus subtilis and yeast in a mass ratio of 10:3:1: 1.

7. The fermented soybean protein feed according to claim 1, which is prepared by the following steps:

(1) preparation of fermentation source bacteria

Mixing fresh fructus Mali Pumilae residue with lactobacillus, sealing and fermenting at 28-30 deg.C for 20-30h, adding yeast and Bacillus subtilis, stirring, and fermenting at 32-34 deg.C for 2-3 days;

(2) making soybean milk

Mixing semen glycines with 3-5 times of clear water, soaking at 30-35 deg.C for 20-30h, freezing at-25 deg.C for 10-15h, taking out the mixture, decocting at 100-110 deg.C for 30-50min, and grinding into slurry;

(3) fermentation of feed

Mixing fresh soybean dregs, corn flour, bran, corn bran, baking soda, livestock salt and soybean milk uniformly, adjusting the water content of the mixture to 55-65%, adding fermentation source bacteria, fermenting at 25-28 ℃ for 3-5 days, then raising the temperature to 30-32 ℃, and fermenting for 4-6 days.

Technical Field

The invention relates to the technical field of feeds, in particular to a soybean protein fermented feed and a preparation method thereof.

Background

With the rapid development of the breeding industry and the feed industry, China becomes one of the largest breeding production countries in the world. At present, the serious shortage of protein feed and the abuse of antibiotics in China become a major bottleneck of the development of feed and breeding industry, so that the finding of high-quality and high-efficiency plant protein raw materials, the reduction of the cost of breeding feed and the reduction of the occurrence of diseases of bred animals become problems to be solved in the development of feed and breeding industry in China.

Because the traditional feed processing does not basically involve biochemical changes, and the microbial fermentation is a highly concentrated biochemical reaction process, the raw materials of the plant protein feed can be deeply fermented and predigested in vitro through the metabolism of beneficial microbes, a large number of toxic and harmful substances, anti-nutritional factors and other substances in the plant protein feed can be effectively degraded and converted into organic acids, polypeptides, amino acids, vitamins, growth promoting factors and other substances required by animal growth, so that the biological feed or the biological protein feed with rich nutrition, high digestion utilization rate, good palatability and high viable bacteria content is formed, the addition amount of fish meal or part of antibiotic medicines in the feed can be effectively reduced or replaced, the animal immunity is improved, the animal growth is promoted, and the occurrence of breeding diseases is reduced. However, the traditional common bioprotein feed starter has the problems of relatively single bacteria, low activity, slow material fermentation start, incomplete degradation of harmful substances, more harmful mixed bacteria, incapability of ensuring the quality, safety, sanitation, feeding effect and the like of bioprotein feed.

Disclosure of Invention

Based on the problems, the invention provides a soybean protein fermented feed which comprises the following components in parts by mass: 25-50 parts of fresh soybean dregs, 20-30 parts of corn flour, 15-30 parts of bran, 15-30 parts of corn bran, 1-3 parts of baking soda, 1-3 parts of livestock salt, 10-30 parts of soybean milk and 5-10 parts of fermentation source bacteria.

Preferably, the feed comprises the following components in parts by mass: 33 parts of fresh soybean dregs, 29 parts of corn flour, 17 parts of bran, 19 parts of corn bran, 2 parts of baking soda, 2 parts of livestock salt, 23 parts of soybean milk and 7 parts of fermentation source bacteria.

Preferably, the fresh soybean dregs contain 85-88% of water and 2-3% of protein.

Preferably, the fineness of the corn flour is 30-50 meshes, and the water content is 5-10%.

Preferably, the corn bran has a particle size of 1-3 mm.

Preferably, the fermentation source bacteria are prepared from apple pomace, lactic acid bacteria, bacillus subtilis and saccharomycetes in a mass ratio of 10:3:1: 1.

The soybean protein fermented feed disclosed by the invention is prepared by the following steps:

(1) preparation of fermentation source bacteria

Mixing fresh fructus Mali Pumilae residue with lactobacillus, sealing and fermenting at 28-30 deg.C for 20-30h, adding yeast and Bacillus subtilis, stirring, and fermenting at 32-34 deg.C for 2-3 days;

(2) making soybean milk

Mixing semen glycines with 3-5 times of clear water, soaking at 30-35 deg.C for 20-30h, freezing at-25 deg.C for 10-15h, taking out the mixture, decocting at 100-110 deg.C for 30-50min, and grinding into slurry;

(3) fermentation of feed

Mixing fresh soybean dregs, corn flour, bran, corn bran, baking soda, livestock salt and soybean milk uniformly, adjusting the water content of the mixture to 55-65%, adding fermentation source bacteria, fermenting at 25-28 ℃ for 3-5 days, then raising the temperature to 30-32 ℃, and fermenting for 4-6 days.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the invention, through soaking and freezing treatment of soybean milk, soybean cells are frozen at low temperature after absorbing water and expanding, the cell walls of the soybean cells are cracked by rapid expansion of ice crystals, and the fiber structure of the soybean milk cells is changed, so that the dissolution efficiency of the prepared soybean milk nutrient substances is higher, the fiber fineness in the soybean milk is further improved, the content of soybean milk dietary fibers is effectively increased, and the fermented soybean milk fibers have better swelling property and digestion resistance, promote the gastrointestinal peristalsis of animals, and effectively improve the activity of gastrointestinal microorganisms.

(2) According to the invention, through the selection of the raw materials, the fermentation of microorganisms is effectively promoted, and the propagation of saccharomycetes, lactic acid bacteria and bacillus subtilis is effectively promoted by utilizing the distributed fermentation of temperature faults, so that the glycolysis degree of the raw materials is improved; the microbial protein of the fermented feed is obviously increased, the microbial activity polysaccharide and the microbial secretion metabolite are obviously improved, the high-efficiency field planting of probiotics in animal intestinal tracts can be efficiently promoted, the utilization rate of the feed by animals is obviously improved through the increase of the nutritional ingredients in the feed and the enhancement of the microbial activity, the absorption and accumulation of the nutritional ingredients of the animals are accelerated, and the aim of improving the content of the nutritional ingredients in animal meat products is further fulfilled.

(3) The invention effectively considers the content ratio of sugar and nitrogen in the raw materials, shortens the adaptation period of the flora in microbial fermentation, has quick temperature rise in the fermentation, high fermentation efficiency of the raw materials, good uniformity of the prepared feed, improved palatability and obviously increased food intake of animals.

Drawings

FIG. 1: cell adhesion statistical plots.

Detailed Description

Example 1:

a soybean protein fermented feed comprises the following components in parts by mass: 33 parts of fresh soybean dregs, 29 parts of corn flour, 17 parts of bran, 19 parts of corn bran, 2 parts of baking soda, 2 parts of livestock salt, 23 parts of soybean milk and 7 parts of fermentation source bacteria; the fresh soybean dregs contain 85% of water and 2% of protein; the fineness of the corn flour is 30 meshes, and the water content is 5%; the granularity of the corn bran is 1 mm; the fermentation source bacteria are prepared from apple pomace, lactic acid bacteria, bacillus subtilis and saccharomycetes in a mass ratio of 10:3:1: 1.

The soybean protein fermented feed disclosed by the invention is prepared by the following steps:

(1) preparation of fermentation source bacteria

Mixing fresh fructus Mali Pumilae residue with lactobacillus, fermenting at 28 deg.C for 20 hr under sealed condition, adding yeast and Bacillus subtilis, stirring, and fermenting at 32 deg.C for 2 days;

(2) making soybean milk

Mixing semen glycines with 3 times of clear water, soaking at 30-35 deg.C for 20 hr, freezing at-25 deg.C for 10 hr, taking out the mixture, decocting at 100 deg.C for 30min, and grinding into slurry;

(3) fermentation of feed

Mixing fresh soybean dregs, corn flour, bran, corn bran, sodium bicarbonate, livestock salt and soybean milk uniformly, adjusting the water content of the mixture to 55%, adding fermentation source bacteria, fermenting at 25 ℃ for 3 days, raising the temperature to 30 ℃, and fermenting for 4 days.

Example 2

A soybean protein fermented feed comprises the following components in parts by mass: 50 parts of fresh soybean dregs, 30 parts of corn flour, 30 parts of bran, 30 parts of corn bran, 3 parts of baking soda, 3 parts of livestock salt, 30 parts of soybean milk and 10 parts of fermentation source bacteria. The fresh soybean dregs contain 88 percent of water and 3 percent of protein; the fineness of the corn flour is 50 meshes, and the water content is 10%; the granularity of the corn bran is 3 mm; the fermentation source bacteria are prepared from apple pomace, lactic acid bacteria, bacillus subtilis and saccharomycetes in a mass ratio of 10:3:1: 1.

The soybean protein fermented feed disclosed by the invention is prepared by the following steps:

(1) preparation of fermentation source bacteria

Mixing fresh fructus Mali Pumilae residue with lactobacillus, fermenting at 30 deg.C for 30 hr under sealed condition, adding yeast and Bacillus subtilis, stirring, and fermenting at 34 deg.C for 3 days;

(2) making soybean milk

Mixing semen glycines with 5 times of clear water, soaking at 35 deg.C for 30 hr, freezing at-25 deg.C for 15 hr, taking out the mixture, decocting at 110 deg.C for 50min, and grinding into slurry;

(3) fermentation of feed

Mixing fresh soybean dregs, corn flour, bran, corn bran, sodium bicarbonate, livestock salt and soybean milk uniformly, adjusting the water content of the mixture to 65%, adding fermentation source bacteria, fermenting at 28 ℃ for 5 days, then raising the temperature to 32 ℃, and fermenting for 6 days.

Example 3

A soybean protein fermented feed comprises the following components in parts by mass: 32 parts of fresh soybean dregs, 21 parts of corn flour, 19 parts of bran, 29 parts of corn bran, 2 parts of baking soda, 1 part of livestock salt, 30 parts of soybean milk and 5 parts of fermentation source bacteria. The fresh soybean dregs contain 88 percent of water and 2 percent of protein; the fineness of the corn flour is 50 meshes, and the water content is 10%; the granularity of the corn bran is 1 mm; the fermentation source bacteria are prepared from apple pomace, lactic acid bacteria, bacillus subtilis and saccharomycetes in a mass ratio of 10:3:1: 1.

The soybean protein fermented feed disclosed by the invention is prepared by the following steps:

(1) preparation of fermentation source bacteria

Mixing fresh fructus Mali Pumilae residue with lactobacillus, fermenting at 30 deg.C for 20 hr under sealed condition, adding yeast and Bacillus subtilis, stirring, and fermenting at 34 deg.C for 2 days;

(2) making soybean milk

Mixing semen glycines with 5 times of clear water, soaking at 30 deg.C for 20 hr, freezing at-25 deg.C for 15 hr, taking out the mixture, decocting at 100 deg.C for 30min, and grinding into slurry;

(3) fermentation of feed

Mixing fresh soybean dregs, corn flour, bran, corn bran, sodium bicarbonate, livestock salt and soybean milk uniformly, adjusting the water content of the mixture to 65%, adding fermentation source bacteria, fermenting at 28 ℃ for 3 days, then raising the temperature to 30 ℃, and fermenting for 4 days.

Test example 1 intestinal flora adhesion test of the feed of the present invention

Healthy mouse intestinal epidermal skin cells were collected and immediately suspended in a centrifuge tube containing 10mL of PBS buffer solution, and the cell concentration was adjusted to 10⁵Per mL; the preparation concentration is 2 multiplied by 10⁷1mL of the bifidobacterium bacterial liquid of cfu/mL is respectively taken and uniformly mixed with the same amount of cell suspension liquid, and the mixture is divided into 10 groups which are marked as a1-a5 and b1-b 5; in a1-a3, 1574ml of the 0.3 percent solution of the feed of the examples 1-3 is added; b1 adding 1ml of CN201610042962.2 feed 0.3% solution. Shake culturing at 37 deg.C for 1h, centrifuging at 1000r/min for 5min, washing with PBS buffer solution for 3 times, discarding bacteria not adhered to cells, smearing the precipitate, drying, gram staining, randomly counting 50 epithelial cells under oil lens, counting the number of bacteria adhered to epithelial cells, and calculating the average value.

The experimental results are shown in figure 1:

it can be seen that the adhesion number of the bifidobacteria is obviously higher than that of the group b1 by using the group a1-3 added with the drug solution, and the drug of the invention can improve the colonization of the cells on the surface of the intestinal tract.

Experimental example 2 animal feeding experiment of the present invention:

and (3) test observation:

selecting 60 meat rabbits with the same age of half a day, randomly dividing the meat rabbits into 4 groups, 15 meat rabbits in each group, namely an experiment 1-3 group and a control group, and carrying out conventional feeding adaptation for one week; experiment 1 group the feed of example 1 of the present invention was used; experiment 2 groups used the feed of example 2 of the invention; experiment 3 groups used the feed of example 3 of the invention; the control group used the feed of application No. CN 201610042962.2.

The process is carried out according to the routine of rabbit farms, other management measures are consistent, and the test period is 1 month. The protein content in the rabbit muscle is determined by referring to a Kjeldahl method in the determination of protein in food of GB/T5009.5-2010 national food safety Standard.

Test results

The protein content of each group of rabbit meat is shown in Table 1

TABLE 1 protein content of rabbit meat in each group

Group of	Protein content
		Experiment 1 group	23.24％
Experiment 2 groups	22.97％
		Experiment 3 groups	23.01％
Control group	20.84％

As can be seen from the table, the protein content of the rabbit muscle using the feed of the invention is obviously higher than that of the control group, so the feed of the invention has obvious effect of improving the content of the animal nutrient components.