阅读说明：本技术 一种基于自由流电泳的藻蓝蛋白分离和富集装置 (Phycocyanin separating and enriching device based on free flow electrophoresis ) 是由 郑行 李林声 杨祎 于 2021-09-17 设计创作，主要内容包括：本发明公开了一种基于自由流电泳的藻蓝蛋白分离和富集装置,包括破碎均浆设备和分离富集设备,所述破碎均浆设备包括罐体,所述罐体的上端通过螺栓可拆卸连接有罐盖,所述罐盖的上端固定连接有安装架,所述罐盖上设有进液搅拌机构,所述进液搅拌机构的下端连接有刮料机构,所述安装架的上端设有用于驱动进液搅拌机构转动的驱动机构,所述罐盖上设有两个翻动机构。本发明可在制取含藻蓝蛋白的原浆时,通过边搅拌变向蓝藻原液中通入溶剂的方式,使得溶剂与蓝藻原液混合更均匀,进而使得蓝藻细胞破裂更充分、快速,通过翻动机构,对含藻蓝蛋白的原浆进行翻动,进一步使得蓝藻细胞裂解更充分,使得藻蓝蛋白在原浆中分散更均匀。(The invention discloses a phycocyanin separating and enriching device based on free flow electrophoresis, which comprises a crushing and homogenizing device and a separating and enriching device, wherein the crushing and homogenizing device comprises a tank body, the upper end of the tank body is detachably connected with a tank cover through a bolt, the upper end of the tank cover is fixedly connected with a mounting frame, a liquid inlet stirring mechanism is arranged on the tank cover, the lower end of the liquid inlet stirring mechanism is connected with a scraping mechanism, the upper end of the mounting frame is provided with a driving mechanism for driving the liquid inlet stirring mechanism to rotate, and two turning mechanisms are arranged on the tank cover. According to the invention, when the primary pulp containing phycocyanin is prepared, the solvent is introduced into the blue algae primary liquid while stirring, so that the solvent and the blue algae primary liquid are mixed more uniformly, the blue algae cells are broken more sufficiently and rapidly, and the primary pulp containing phycocyanin is turned by the turning mechanism, so that the blue algae cells are cracked more sufficiently, and the phycocyanin is dispersed in the primary pulp more uniformly.)

1. The utility model provides a phycocyanin separation and enrichment device based on free flow electrophoresis, includes broken homogenate equipment and separation enrichment equipment, a serial communication port, broken homogenate equipment includes jar body (1), the upper end of jar body (1) can be dismantled through the bolt and be connected with cover (2), the upper end fixedly connected with mounting bracket (11) of cover (2), be equipped with feed liquor rabbling mechanism on cover (2), feed liquor rabbling mechanism's lower extreme is connected with scraping mechanism, the upper end of mounting bracket (11) is equipped with and is used for driving feed liquor rabbling mechanism pivoted actuating mechanism, be equipped with two mechanisms that turn on cover (2), and two mechanisms that turn are located feed liquor rabbling mechanism's both sides respectively, two the mechanism that turns all is connected with feed liquor rabbling mechanism through the link gear, the lower extreme of jar body (1) is connected with connecting pipe (19), the connecting pipe (19) is provided with a booster pump (18), and the side wall of the tank body (1) is provided with a liquid injection pipe (14).

2. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 1, wherein the liquid feeding stirring mechanism comprises a second rotating pipe (17) vertically penetrating through the middle of the tank cover (2), the upper end of the second rotating pipe (17) is provided with an opening, the lower end of the second rotating pipe is of a closed structure, the second rotating pipe (17) is rotatably connected with the tank cover (2), the second rotating pipe (17) is positioned on the side wall of the inner side of the tank body (1) and is provided with a plurality of stirring pieces at equal intervals, the mounting rack (11) is vertically penetrated through the first rotating pipe (6), the first rotating pipe (6) is rotatably connected with the mounting rack (11), the upper end and the lower end of the first rotating pipe (6) are of a through structure, and the lower end of the first rotating pipe (6) is fixedly connected with the upper end of the second rotating pipe (17).

3. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 2, wherein said stirring member comprises a U-shaped tube (28) fixedly connected to a side wall of the second rotating tube (17), and an opening of the U-shaped tube (28) faces the second rotating tube (17), said second rotating tube (17) is communicated with the U-shaped tube (28), two inner side walls of the U-shaped tube (28) opposite to each other are fixedly connected with a sliding rail (29), two sliding rails (29) are slidably sleeved with sliding sleeves (31), a stirring blade (33) is fixedly connected between the two sliding sleeves (31), two sliding rails (29) are sleeved with a third spring (30), one end of the third spring (30) is fixedly connected to the sliding sleeves (31), the other end of the third spring (30) is fixedly connected to the sliding rails (29), a plurality of liquid outlet holes (32) are arranged on two opposite inner side walls of the U-shaped pipe (28) at equal intervals.

4. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 2, wherein said scraping mechanism comprises a rotating rod (22) fixedly connected to the lower end of the second rotating tube (17) and horizontally disposed, a scraper (20) parallel to the rotating rod (22) is disposed below the rotating rod (22), and a plurality of second springs (21) are connected between the rotating rod (22) and the scraper (20) at equal intervals.

5. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 2, wherein said driving mechanism comprises a motor (3) fixedly mounted on the upper end of a mounting frame (11), the end of the output shaft of said motor (3) is fixedly connected with a driving wheel (4), a driven wheel (5) is fixedly sleeved on the side wall of said first rotating tube (6) above said mounting frame (11), and said driven wheel (5) is meshed with said driving wheel (4).

6. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 2, wherein the flipping mechanism comprises a movable rod (16) vertically penetrating through the tank cover (2), the movable rod (16) is slidably connected with the tank cover (2), a fixed block (12) is fixedly connected to the upper end of the movable rod (16), a first spring (13) is sleeved on the side wall of the movable rod (16) above the tank cover (2), one end of the first spring (13) is fixedly connected to the fixed block (12), the other end of the first spring (13) is fixedly connected to the tank cover (2), and a plurality of fixed plates (15) are fixedly sleeved on the side wall of the movable rod (16) inside the tank body (1) at equal intervals.

7. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 6, wherein said linkage mechanism comprises a supporting block (7) fixedly sleeved on the first rotating tube (6), and both ends of the supporting block (7) are of arc surface structure, the upper end of the tank cover (2) is symmetrically provided with two limiting sliding grooves (8), two limiting sliding blocks (9) are slidably connected in the two limiting sliding grooves (8), both ends of the supporting block (7) respectively abut against the two limiting sliding blocks (9), both ends of the two limiting sliding blocks (9) are rotatably connected with connecting rods (10), and one ends of the two connecting rods (10) far away from the limiting sliding blocks (9) are respectively rotatably connected with the two fixing blocks (12).

8. The phycocyanin separating and enriching device based on free flow electrophoresis as claimed in claim 1, wherein the separating and enriching apparatus comprises a separating chamber (24), the upper end of the separating chamber (24) is connected with a sampling tube (23), the sampling tube (23) is connected with a connecting tube (19), the lower end of the separating chamber (24) is connected with a plurality of outlet tubes (26) at equal intervals, the outlet tubes (26) are connected with a collecting tube (27), and the side walls of the two sides of the separating chamber (24) are both provided with electrode chambers (25).

Technical Field

The invention relates to the technical field of phycocyanin separation and collection, in particular to a phycocyanin separation and enrichment device based on free flow electrophoresis.

Background

The phycocyanin contained in the blue algae is up to more than 5 percent, the selling price is extremely high, and the phycocyanin of the fluorescent reagent grade is monopolized abroad. The high-purity phycocyanin separated from the blue algae not only can reduce the environmental pollution, but also can generate higher economic benefit.

The existing method generally comprises the steps of injecting a solvent into a blue algae stock solution to break blue algae cells so as to obtain primary pulp containing phycocyanin, separating and enriching the phycocyanin through free flow electrophoresis separation equipment, but when the existing primary pulp containing the phycocyanin is prepared, the dispersion of the phycocyanin in the primary pulp is not uniform, so that the phycocyanin is difficult to be fully separated in the subsequent separation process, the purity of the obtained phycocyanin is low, multiple times of separation and purification are needed, the energy is wasted, and the cost is increased.

Disclosure of Invention

The invention aims to solve the defects in the prior art, and provides a phycocyanin separating and enriching device based on free flow electrophoresis.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a phycocyanin separation and enrichment device based on free flow electrophoresis, includes broken homogenate equipment and separation enrichment equipment, broken homogenate equipment includes a jar body, the upper end of jar body can be dismantled through the bolt and be connected with the cover, the upper end fixedly connected with mounting bracket of cover, be equipped with feed liquor rabbling mechanism on the cover, feed liquor rabbling mechanism's lower extreme is connected with scrapes material mechanism, the upper end of mounting bracket is equipped with and is used for driving feed liquor rabbling mechanism pivoted actuating mechanism, be equipped with two on the cover and turn the mechanism, and two turn the mechanism and be located feed liquor rabbling mechanism's both sides respectively, two it all is connected with feed liquor rabbling mechanism through the link gear to turn the mechanism, the lower extreme of the jar body is connected with the connecting pipe, be equipped with the booster pump on the connecting pipe, be equipped with on the lateral wall of the jar body and annotate the liquid pipe.

Preferably, feed liquor rabbling mechanism includes that vertical running through sets up the second rotating tube at cover middle part, and the upper end of second rotating tube has opening, lower extreme and be the enclosed construction, the second rotating tube rotates with the cover to be connected, the second rotating tube is located jar equidistant a plurality of stirring pieces that are equipped with on the inboard lateral wall of body, vertical running through is equipped with first rotating tube on the mounting bracket, and first rotating tube rotates with the mounting bracket to be connected, the upper and lower both ends of first rotating tube are link up structure, the lower extreme fixed connection of first rotating tube is in the upper end of second rotating tube.

Preferably, the stirring part comprises a U-shaped pipe fixedly connected to the side wall of the second rotating pipe, the opening of the U-shaped pipe faces the second rotating pipe, the second rotating pipe is communicated with the U-shaped pipe, two sliding rails are fixedly connected to two inner side walls opposite to the U-shaped pipe, two sliding sleeves are sleeved on the sliding rails in a sliding mode, two stirring blades are fixedly connected between the sliding sleeves, two third springs are sleeved on the sliding rails, one end of each third spring is fixedly connected to the corresponding sliding sleeve, the other end of each third spring is fixedly connected to the corresponding sliding rail, and a plurality of liquid outlet holes are formed in two inner side walls opposite to the U-shaped pipe at equal intervals.

Preferably, scrape the dwang that material mechanism includes fixed connection at second rotating tube lower extreme and level set up, the below of dwang is equipped with rather than parallel scraper blade, equidistant a plurality of second springs that are connected with between dwang and the scraper blade.

Preferably, the driving mechanism comprises a motor fixedly mounted at the upper end of the mounting frame, a driving wheel is fixedly connected to the tail end of an output shaft of the motor, a driven wheel is fixedly sleeved on the side wall, located above the mounting frame, of the first rotating pipe, and the driven wheel is meshed with the driving wheel.

Preferably, the mechanism that turns includes the vertical movable rod that runs through the setting on the cover, and movable rod and cover sliding connection, the upper end fixedly connected with fixed block of movable rod, the movable rod has cup jointed first spring on being located the lateral wall of cover top, the one end fixed connection of first spring is on the fixed block, the other end fixed connection of first spring is on the cover, the movable rod is located the equidistant fixed cover of the inboard lateral wall of jar and has been connect a plurality of stationary blades.

Preferably, link gear is including fixed cover joint the piece of supporting on first rotation pipe, and the both ends of supporting the piece are ARC structure, the upper end symmetry of cover is equipped with two spacing spouts, two equal sliding connection has spacing slider in the spacing spout, and supports the both ends of piece and offset with two spacing sliders respectively, two all rotate on the spacing slider and be connected with the connecting rod, two the connecting rod is kept away from the one end of spacing slider and is rotated respectively and connect on two fixed blocks.

Preferably, the separation enrichment equipment includes the separation chamber, the upper end of separation chamber is connected with the sampling pipe, the sampling pipe is connected with the connecting pipe, the lower extreme equidistant connection of separation chamber has a plurality of outlet pipes, be connected with the collecting pipe on the outlet pipe, all install the electrode room on the both sides lateral wall of separation chamber.

The invention has the beneficial effects that:

through setting up the liquid injection pipe, feed liquor rabbling mechanism, actuating mechanism, through to the solvent of coming into in the first rotation pipe, through to the intraductal blue alga stock solution that inserts of liquid injection, the starter motor, and then the first rotation pipe of drive rotates, it rotates to drive the second rotation pipe, the solvent flows in the U type pipe, and in getting into the blue alga stock solution through going out the liquid hole, and rotate through the second rotation pipe and drive the stirring leaf and stir the blue alga stock solution, make solvent and blue alga stock solution mix more evenly, and then make the blue alga cell break more fully, it is quick.

Through setting up the mechanism that turns, when first rotation pipe rotates, drive and support the piece and rotate, support when supporting the piece and rotate, support and press spacing slider and remove along spacing spout, and then drive the movable rod through the connecting rod and shift up, and then turn over the blue alga stoste through the stationary blade, will turn over at the blue alga of bottom, further make the schizolysis of blue alga cell more abundant, and then make the dispersion of phycocyanin more even in the magma.

Drawings

FIG. 1 is a schematic structural diagram of a crushing and homogenizing device of a phycocyanin separating and enriching device based on free flow electrophoresis;

FIG. 2 is a schematic structural diagram of a separation and enrichment apparatus of a phycocyanin separation and enrichment device based on free flow electrophoresis according to the present invention;

FIG. 3 is a schematic structural diagram of a stirring member of the phycocyanin separating and enriching device based on free flow electrophoresis;

fig. 4 is a schematic structural diagram of a resisting block of a phycocyanin separating and enriching device based on free flow electrophoresis.

In the figure: 1 can body, 2 cover, 3 motors, 4 driving wheels, 5 driven wheels, 6 first rotating pipes, 7 abutting blocks, 8 limiting chutes, 9 limiting slide blocks, 10 connecting rods, 11 mounting frames, 12 fixing blocks, 13 first springs, 14 liquid injection pipes, 15 fixing plates, 16 movable rods, 17 second rotating pipes, 18 booster pumps, 19 connecting pipes, 20 scraping plates, 21 second springs, 22 rotating rods, 23 sample inlet pipes, 24 separation cavities, 25 electrode chambers, 26 outlet pipes, 27 collecting pipes, 28U-shaped pipes, 29 sliding rails, 30 third springs, 31 sliding sleeves, 32 liquid outlet holes and 33 stirring blades.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-4, a phycocyanin separation and enrichment device based on free flow electrophoresis, including broken homogenate equipment and separation enrichment equipment, broken homogenate equipment includes jar body 1, the upper end of jar body 1 can be dismantled through the bolt and be connected with cover 2, the upper end fixedly connected with mounting bracket 11 of cover 2, be equipped with feed liquor rabbling mechanism on the cover 2, the lower extreme of feed liquor rabbling mechanism is connected with scrapes the material mechanism, the upper end of mounting bracket 11 is equipped with and is used for driving feed liquor rabbling mechanism pivoted actuating mechanism, be equipped with two mechanisms that turn on cover 2, and two mechanisms that turn are located the both sides of feed liquor rabbling mechanism respectively, two mechanisms that turn all are connected with the feed liquor rabbling mechanism through the link gear, the lower extreme of jar body 1 is connected with connecting pipe 19, be equipped with booster pump 18 on the connecting pipe 19, be equipped with on the lateral wall of jar body 1 and annotate liquid pipe 14.

According to the invention, the liquid feeding stirring mechanism comprises a second rotating pipe 17 vertically penetrating through the middle part of the tank cover 2, the upper end of the second rotating pipe 17 is provided with an opening, the lower end of the second rotating pipe 17 is of a closed structure, the second rotating pipe 17 is rotatably connected with the tank cover 2, a plurality of stirring pieces are arranged on the side wall of the second rotating pipe 17, which is positioned on the inner side of the tank body 1, at equal intervals, a first rotating pipe 6 vertically penetrates through the mounting frame 11, the first rotating pipe 6 is rotatably connected with the mounting frame 11, the upper end and the lower end of the first rotating pipe 6 are of a through structure, and the lower end of the first rotating pipe 6 is fixedly connected to the upper end of the second rotating pipe 17.

Stirring spare includes U type pipe 28 of fixed connection on second rotating tube 17 lateral wall, and the opening of U type pipe 28 is towards second rotating tube 17, second rotating tube 17 and U type pipe 28 intercommunication, equal fixedly connected with slide rail 29 on two relative inside walls of U type pipe 28, equal slip has cup jointed sliding sleeve 31 on two slide rails 29, fixedly connected with stirring leaf 33 between two sliding sleeves 31, third spring 30 has all been cup jointed on two slide rails 29, the one end fixed connection of third spring 30 is on sliding sleeve 31, the other end fixed connection of third spring 30 is on slide rail 29, impartial interval is equipped with a plurality of liquid holes 32 on two relative inside walls of U type pipe 28, steerable motor 3's rotational speed, and then adjust the rotational speed of first rotating tube 6, along with the difference of rotational speed, the centrifugal force of production is different, and then make stirring leaf 33 remove along slide rail 29, make the stirring abundant.

Scraping mechanism includes fixed connection at the dwang 22 of second rotating tube 17 lower extreme and level setting, and the below of dwang 22 is equipped with rather than parallel scraper blade 20, and equidistant a plurality of second springs 21 of being connected with between dwang 22 and the scraper blade 20 can scrape the blue alga of bottom and be convenient for upwards turn.

Actuating mechanism includes motor 3 of fixed mounting in the upper end of mounting bracket 11, and the terminal fixedly connected with drive wheel 4 of the 3 output shafts of motor, first rotation pipe 6 are fixed the cup joint on the lateral wall that is located the mounting bracket 11 top have from driving wheel 5, follow the meshing of driving wheel 5 and drive wheel 4.

Further, the mechanism of stirring includes that the vertical movable rod 16 that runs through the setting on cover 2, and movable rod 16 and cover 2 sliding connection, the upper end fixedly connected with fixed block 12 of movable rod 16, first spring 13 has been cup jointed on the lateral wall that movable rod 16 is located cover 2 top, the one end fixed connection of first spring 13 is on fixed block 12, the other end fixed connection of first spring 13 is on cover 2, movable rod 16 is located the fixed a plurality of stationary blades 15 that have cup jointed of equidistant on the lateral wall of the internal side of jar 1.

The link gear is including fixed cup joint piece 7 of supporting on first rotation pipe 6, and the both ends of supporting piece 7 are cambered surface structure, the upper end symmetry of cover 2 is equipped with two spacing spouts 8, equal sliding connection has spacing slider 9 in two spacing spouts 8, and the both ends of supporting piece 7 offset with two spacing sliders 9 respectively, it is connected with connecting rod 10 all to rotate on two spacing sliders 9, the one end that spacing slider 9 was kept away from to two connecting rod 10 rotates respectively and connects on two fixed blocks 12, turn the mechanism through setting up, and then turn the blue alga stoste through stationary blade 15, turn over at the blue alga of bottom, further make blue alga cell schizolysis more abundant, and then make the dispersion of phycocyanin more even in the magma.

The separation enrichment equipment includes separation chamber 24, separation chamber 24's upper end is connected with into appearance pipe 23, it is connected with connecting pipe 19 to advance appearance pipe 23, the lower extreme equidistant connection of separation chamber 24 has a plurality of outlet pipes 26, be connected with collecting pipe 27 on the outlet pipe 26, all install electrode chamber 25 on the both sides lateral wall of separation chamber 24, after obtaining containing phycocyanin magma, accessible connecting pipe 19 will contain the input of phycocyanin's magma and enter separation enrichment equipment, start electrode chamber 25, make the phycocyanin strip flow out at specific outlet pipe 16, collect through collecting pipe 27.

When the invention is used, a solvent is put into the first rotating pipe 6, a blue algae stock solution is put into the liquid injection pipe 14, the motor 3 is started, the first rotating pipe 6 is driven to rotate, the second rotating pipe 17 is driven to rotate, the solvent flows into the U-shaped pipe 28 and enters the blue algae stock solution through the liquid outlet 32, the stirring blade 33 is driven to stir the blue algae stock solution through the rotation of the second rotating pipe 17, the solvent and the blue algae stock solution are mixed more uniformly, the blue algae cells are broken more sufficiently and rapidly, the abutting block 7 is driven to rotate when the first rotating pipe 6 rotates, the abutting block 7 moves along the limiting chute 8 when rotating, the connecting rod 10 drives the movable rod 16 to move upwards, the blue algae stock solution is turned over through the fixed plate 15, the blue algae at the bottom layer is turned over, the blue algae cells are further cracked more sufficiently, and the blue algae proteins are further dispersed more uniformly in the primary pulp, after obtaining the primary pulp containing phycocyanin, the primary pulp containing phycocyanin can be input into the separation and enrichment equipment through the connecting pipe 19, the electrode chamber 25 is started, so that the phycocyanin strip flows out at the specific outlet pipe 16 and is collected through the collecting pipe 27.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.